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Biorad PW40 PW41 manual ENG

1. we a 12 09 96 BE 3 ELS Ges E 5 er Bel Bes cool Roa me tm z fwoo 50 ma T TI CRIE LT v zb 008 HE a w Dio m ols ol Sie D S 88182188 o 912 210 Fla 2199 2 Di 5 9 as 9 gt 1818115 Sim SIE se 2 0 9 5 3 5 o 215 8 o m gt 310 us 3 9 sla es Za 8 2 2 5 E m 215 4 lt 9 gt 3 515 e 9 5 5 Pla nz ja vlz gt 5 aim gt gt 2 D 5 5 gle r m gt o o o o lt so gt 2 s amp 5 5 a 5 51 a 5 o cp AEG E 2 3 o 291 375 5 5 8 5 m 0 3 E D 3 S velsion duly 1998 User Manual 2050 09018 apoo renueyi HN HF d 8661 Alnp UOIS18A Aspiration horizontal position ASP POS Plate centering CENTERING Aspiration vertical position ASP VERT POS Bottom aspiration vertical position BOT VERT POS Bottom Wash vertical position B W VERT POS HORIZONTAL SPEED VERTICAL SP
2. 3 30 3 7 8 8 COPY PLATE 3 31 2 INSTALLATION 2 1 3 7 84 ERROR on PLATE 3 31 2 1 Unpacking 21 3 79 Plate Test 3 32 3 7 10 RAM initialization 3 33 3 OPERATING INSTRUCTIONS 3 1 4 MAINTENANCE amp 3 1 Run Mode 3A TROUBLESHOOTING 4 1 3 1 1 How to use the Keyboard 3 1 4 1 Routine maintenance 3 2 Microplate loading 3 2 Procedures 4 1 3 3 Select one KIT 3 3 41 1 Daily Maintenance 4n 34 WASH interruption or 4 1 2 Washer storage conditions 4 1 Power Failure 3 5 4 2 Cleaning and disinfection 4 2 3 4 1 WASH interruption 3 5 421 Cleaning the manifold 4 2 3 4 2 POWER Failure 3 5 422 Disinfection 4 3 3 5 RINSE 3 6 4 3 Troubleshooting 4 4 3 5 1 RINSE with 40 3 6 4 3 1 Hardware and software version 4 4 3 5 2 Please RINSE with PW40 3 6 3 5 3 RINSE with PW41 3 7 Appendix 3 5 4 Automatic Rinsing with PW41 3 7 Accessories and 5 36 Configuration 3 8 spare list 3 6 1 Main menu 3 8 PW40 Wash protocols 2 3 6 2 Washer s configuration 3 9 PW41 Wash protocols A 6 E 3 7 PROGRAMMING 3 12 Recommendations for 3 71 Kit Structure 3 12 optimized washing conditions 10 3 7 2 Access to Revision Upgrade Description 11 L PROGRAMMING mode 3 13 Index 12 is i LI Version July 1998 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 IMPORTANT Please read carefully before using the PLATE WASHERS PW40
3. Version July 98 3 13 User Manual code 87050 Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 3 OPERATING INSTRUCTIONS 3 7 PROGRAMMING continued 3 7 3 Programming Synopsis PRG ADD See 83 7 4 ADD METHOD ADD PLATE PRG INSERT INSERT KIT See 3 7 5 INSERT METHOD INSERT PLATE PRG DELETE DELETE KIT See 83 7 6 DELETE DELETE PRG EDIT EDIT KIT See 3 7 7 EDIT PLATE PRG COPY COPY KIT See 3 7 8 COPY PLATE PRG PLATE TEST RUN KIT 75 See 3 7 9 Version July 98 User Manual code 87050 3 14 Fig 16 wp CC Plate Washers PW40 amp PW41 Ul Manu em Codes 85499 amp 85508 3 OPERATING INSTRUCTIONS 3 7 PROGRAMMING continued 3 7 4ADD 3 7 4 1 ADD KIT The kit is added atthe end of the existing kit list Use T and keys to scroll the available menu Press YES key to initiate the dis played selection Press ESC key to escape and go back to former prompt Enter kit name select character with T and 4 keys then press key to move to next charac ter up to 15 characters NAME KIT Nr2 t 4 gt PLATEOI Select plate to be associated to kit t YESESC Select manifold type PWAO 8 or 12 channels PW41 8 2 8 3 8 or 12 2 12 channels MANIFOLD STRIP 1 X 3 Omit strip pla
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5. mer lows met Exp i EARJE EE n ________________ _ ________ pM repere receperit mm e e peser po EL S NIKON NON MSN ae om Lenze Be EE ELM EOD d s seres so soan zos gp BON Dee Bee ip mcr om E pL reb Ep pe p p a mee mo 2 z o mio ojo 9 gt gt 0 ae 2 ES 198 SIS 219 2 8 9 212 219 sls 2 3 ER A T _ T A Oj 9 9 slo o x D TERIER ER RC S 3 2 5 mz 3 in PIE Slo 3 lo 91551720 H 2 0 2 5 m gt 5 D T D 2 z 8 lt 0 gt 8 iu 5 21 s 2 5803172 lt 8 z Jiz Z 8 5 S m 8 D 5 8 m3 5 o E 5 5g Ei 3 38 pla e w esnels lt S Pasteur User Manual oodes 85499 6 85608 Appsndix e Kass
6. Plate Washers PW40 amp PW41 Sanofi Diagnostics Pasteur User Manual 0 27 22 ____ 85499 amp 85508 Appendix Index continued M MAIN PARAMETERS 3 27 Maintenance 4 1 Manifold 1 1 MET INTER 1 11 Method 1 10 1 11 Method Interval 1 11 Microplate loading 3 2 Mini Priming 3 1 1 Omit strip 3 15 OUT 3 1 OVERFLOW 1 13 Overflow Position 1 20 Physical data 1 7 Plate Agitation 1 19 Plate Carrier 1 1 1 4 PLATE mode 1 10 PLATE parameter 1 12 PLATE TEST 3 32 Please RINSE 3 6 Prime 3 3 Programing Synopsis 3 14 PUMP PRIMING 3 8 PWAO Basic features 1 1 PWAO Hydraulic circuit 7 3 40 Outside dimensions 1 2 PW41 Basic features 1 4 PW41 Hydraulic circuit 1 6 41 Outside dimensions 1 5 R RAM initialization 3 33 Routine maintenance 4 1 RS232 serial port for connection to PC 1 4 Version July 1998 User Manual code 87050 S SAFETY INFORMATION 5 Secured Stop 3 11 SHAKE TIME 1 19 SHAKING AMPLITUDE 1 19 SHAKING SPEED 1 19 Soaktime 1 11 SOAKING 1 11 Software parameters for Wash protocols 1 9 Software specifications 1 8 Software version 4 4 STRIP mode 1 10 T Technical features 1 7 Temporary Kit 3 23 Trap Vial 1 3 Troubleshooting 4 4 u Unpacking 2 1 V Vacuum sensing 1 6 Vertical speed 3 20 VOLUME 1 13 W WARNING 5 WASH 1 14 WASH ASP 1 9 1 10 Wash Aspiration 1 10 Wash Bottom Aspiration 1 10 WASH BOT ASP 1 9 1 10
7. Programmed parameters Kit parameters CROSW Crosswise aspiration CWA for flat bottom only YES or NO the aspiration needle is shifted close to the wall Aspiration Horizon tal Position while going down to well bottom and aspirates once the aspiration time has elapsed the aspiration needle is going up to ap prox middle of well height and is shifted to opposite end of wall no scratch on the bottom the needle goes down to bottom and 5 cond aspiration sequence takes place ASP TIME Aspiration time 0 1 to 9 9 sec step 0 1 sec Time during which the aspirating needie is kept at the well bottom and is aspirating Plate parameters ASP HOR POS Aspiration Horizontal Position for flat bottom only 0 0 centered to 2 0 mm step 0 1 mm see also fig 8 at the end of 1 8 ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of 1 8 0 1 is the highest needle position 15 0 is the lowest needle position DOWNW SPEED Aspiration Downward Speed 0 to 9 step 1 speed mini 0 speed max 9 Version July 1998 User Manual code 87050 1 12 C CU Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual 499 1 FEATURES AND SPECIFICATIONS 18 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued D DISPENSING dispensing needle is placed over the well
8. keyto move to next cha racter up to 8 characters Indicate well bottom shape FLAT or CURVED FLAT YESESC SHAPE 4 BOT iE Aspiration horizontal position for FLAT bottom only See definition on para 1 8 3 POS 1 4 mm YESESC ASP HOR 1 l Centering of the opiate wells rela tively to manifold needle in the In Out direction 2 0mm IN to 2 0 mm OUT step 0 1 mm Important parameter when a crosswise aspiration must be made close to the wall of wells Aspiration vertical position See definition on para 1 8 3 18 5mm YESESC ASP VERT POS j30 to page 3 2cP Version July 98 3 19 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 4 User Manual de OPERATING INSTRUCTIONS 3 7 PROGRAMMING continued 3 7 4ADD continued 3 7 4 5 ADD PLATE continued continuation from page 3 19 11 8mm BOT VERT POS HORIZONTAL SPEED 6 t i YESESC VERTICAL SPEED 6 t 4 YESESC ASP DOWNW SPEED J30 to page 3 2 Bottom aspiration vertical posi tion See definition on para 1 8 3 Bottom Wash Vertical Position See definition on para 1 8 3 Horizontal speed In Out plate carrier speed during non work movements movement to home position re turn movement from last to first strip etc Oto 9 step 1 speed mini 0 s
9. PW40 amp PW41 Codes 85499 amp 85508 1 FEATURES AND SPECIFICATIONS PWAO 13 PWA40 Hydraulic circuit 2 SE 58 lt 8 5 B 5 lt O 2 S KH 9 5 e S 5 3 gt gt I d e 5 is E a 55 8 12 o 2 Fig 3 I e L Version July 1998 User Manual code 87050 18 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 854 8SS08 1 FEATURES AND SPECIFICATIONS PWA1 14 PWA41 Basic features Waste bottle 2x20 characters LCD Display and 5 soft Keys Aerosol Protection Cover 85232 serial port for connection to PC 4 liquid inputs 3 Wash bottles 1 Rinse bottle Manifold 2 8 Channel Removable 8 Mode 12 Mode Plate Carrier Removable Autoclavable at 120 250 Fig 4 Version July 1998 User Manual code 87050 1 4 canon Sr Pasteur Plate Washers PW40 amp PW41 1 FEATURES AND SPECIFICATIONS PW41 15 PW41 Outside dimensions mm Fig 5 Version July 1998 1 5 User Manual code 87050 Diagnostics Pasteur PWA1 16 Liquid pump Electro valve WASH or RINSE 2 8 Channel Manifold Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 FEATURES AND SPECIFICATIONS PW41 Hydraulic circuit Liquid fevel detection al Input 3 c Lamy VENE
10. PW41 This document is the copyright of SANOFI DIAGNOSTICS PASTEUR and must not be copied or reproduced in any form without prior consent SANOFI DIAGNOSTICS PASTEUR reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous pro gram or product development This manual supersedes all previous editions _ 3 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 About this manual This manual has been written for the laboratory technician and describes how to use the PW40 and PW41 plate washers This manual is designed to give you the information you need to install the PW40 or the PW41 operate with the PW40 or the PW41 to suit your particular needs perform the PW40 or the PW41 basic maintenance procedures including disinfection This manual also describes all the features and specifications of the PW40 and PW41 hardware and configuration software You will find explanations of all the error messages and a D bb solving guide in the PW troubleshooting guide Version July 1998 4 User Manual code 87050 Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 SAFETY INFORMATI WARNING To operate this instrument must be mandatory connected to a ELECTRIC wall socket equipped with earth
11. PWAO 85499 PW41 85508 anuel d Utilisation Manet esee User Manual al Revision July 1998 Software versions 2 XX 2Y for PW40 and PW41 hc on 08 00 27 VB nt 2 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 TABLE OF CONTENTS About this manual 4 3 7 3 Programming Synopsis 3 14 SAFETY INFORMATION 5 3 74 ADD 3 15 SAFETY SYMBOLS 6 3 7 4 4 ADD KIT 3 15 3 7 4 5 ERRORS on KIT programming 3 16 1 FEATURES AND Sag ADD METHOD SR SPECIFICATIONS 11 ON 11 PWAO Basic features 1 1 METHOD programming E 9 7 4 5 ADD PLATE 3 19 12 40 Outside dimensions 1 2 375 INSERT 3 23 13 40 Hydraulic circuit 13 3 7 51 INSERT KIT 3 23 14 Basic features 14 3 7 5 2 TEMPORARY KIT 3 23 15 PWA1 Outside dimensions mm 1 5 3 7 5 3 INSERT METHOD 3 23 1 6 PW41 Hydraulic circuit 1 6 3 7 5 4 INSERT PLATE 3 24 1 7 Technical features 1 7 37 6 DELETE 3 25 171 Electrical Data 1 7 3 7 6 1 DELETE KIT 3 25 172 Physical data _ 17 3 7 6 2 DELETE METHOD 3 25 1 7 3 Hardware specifications 1 7 37 63 DELETE PLATE 3 26 174 Software specifications 1 8 377 EDIT 3 27 18 Software parameters for Wash 37 71 EDIT KIT 3 27 protocols 19 3 7 7 2 EDIT PLATE 3 29 1 8 1 WASH protocol definition 19 3 78 COPY 3 30 1 8 2 Methods 1 10 3781 COPY KIT 3 30 1 8 3 Parameter s definitions 1 12 3 7 8 2 ERROR on KIT
12. Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85 FEATURES AND SPECIFICATION 18 Software parameters for Wash protocols continued 1 8 8 Parameter s definitions continued w Bottom Washing BOTTOM the bottom of the well is thoroughly washed followed by a normal WASH Wash W sequence Following sequences are applied Downward aspirating movement of the needle into the well down to Aspiration Vertical Position Aspiration at Aspiration Vertical Position during Aspiration Time One or two upward dispensing movements up to Bottom Wash Ver tical Position dispensing at this position during a Bottom Wash Time followed by downward aspirating movement down to Aspiration Vertical Position Aspiration at Aspiration Vertical Position during Aspiration Time Dispensing up to Overflow Position In case of crosswise aspiration for flat bottom only every aspiration sequence close to the wall is immediately followed by an other aspiration sequence to the opposite end of wall as descri bed on page 1 10 Programmed parameters Kit parameters CROSW ASP Crosswise aspiration YES or NO for flat bottom only See details page 1 10 ASP TIME Aspiration time 0 1 to 9 9 sec step 0 1 sec See details page 1 10 VOLUME for one well 50 to 3000 VUL step 50 uL the well volume is approx 370 lil Volume is applied at the last Dispensing sequence OV
13. input 2i N p solution Inputs Hydrophobic filter Paez 552526955553 WASTE Fig 6 ul Hc Version July 1998 User Manual code 87050 1 6 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual FEATURES AND SPECIFICATIONS 1 7 Technical features 1 7 1 Electrical Data Voltage 240 100 VAC 50 60 Hz Consumption 100 VA max Fuses 2 5 AT 2 fuses 5x20 mm Power Cord on CEI socket 1 7 2 Physical data Dimensions including bottles Width 551 mm PW40 822 mm PW41 Length 530 mm Plate carrier out PW40 amp PW41 Height 300 mm PW40 395 mm PW41 Weight 11 0 PW40 without accessories 11 6 Kg PW41 Operating conditions 15 30 15 85 R H 1 7 3 Hardware specifications Manifold PW40 12 channel Default 8 channel PWA1 8 2 8 3 8 12 or 2 12 channel Default 2 8 1 integrated vacuum pump maxi 8 L min 2 integrated vacuum pumps maxi 16 L min Vacuum power PWAO PW41 Volume of WASTE bottle PW40 2 litres PW41 8 litres User interface Flat keyboard with 5 diaphragm keys 2x20 characters LCD screen backlighted onPW41 User Manual code 87050 17 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 1 FEATURES AND SPECIFICATION 17 Technical features continued 1 7 4 Software specifications Kits Up to 75 washing kits progra
14. removed Then press YES CONNECT THE DISINF PUMP PRIMING ESC DISINFECTION Pumps are stopped whereas 0H30mn00s ESC dispensing circuits are filled with disinfection solution countdown of 30 mn is started after which you are prompted to perform a RINSE operation IMPORTANT The final RINSING sequence must be performed in all cases after the disinfection procedure has been started even for dem onstration purpose The software uses this final rins ing sequence to Rinse liq uid at all the 4 inputs The automatic rinsing will not be possible ERR 18 if rinsing is not done after disinfection CONNECT THE RINSE Remove disinfection solution and ON A YESESC fill RINSE bottle with deionized water DiH2O Then press YES Then prior launching a wash pro tocol assign the correct Wash so a lution to the wash input s in the CONFIGURATION menu or by priming the circuit with the PRIM ING RINSING menu Backto main menu IMPORTANT Avoid bleach for disinfecting the washer as a thorough rinsing ofthe tubes will not prevent traces of bleach to remain inside tubing and manifold This can be enough to corrupt reagents of next washing Disinfect the washer when it has not been used for more than one day When used daily disinfect it at least once a week Disinfect the washer prior to any servicing or handling action ship ment or change of site V
15. 0 Sal Sa oN 77008 LOL ON id 11008 N LOL IE e Siet 7000 N LOL 0 11008 EN LOL 210008 ZN 301 08 S EN 17008 79008 ZN LOL K 52 EE ES 10 1814 77008 ZN LOL 11008 IN t0 UN LAN PON dSV LO m 008 ER ES M SEO eld 10 1814 350 seA Se WOLLOS euin HSVA 108 3WIL dSV 8 euin S IER JequinN uionog uonesueduioo Mo uonisod euinjoA pesuedsiq dSv MsSOHO zs poywo duis ANVN eureu NZ oodo 82060 ve sjon du v 1998 Piste Wasners v ve Sanor Blegnos jos pesjeul User Manual oodes 8 99 8 85508 Appendix 2 09 96 40 Kit sheet sz 550 ON EJ MOOG ON OLL hht eme 7 9 91014 1 NOLL EARS EES tO Eld 14008 EN 011 TRUE PE e a ee pa ps ee epe 8011 91 pev ou peo om He EC ps passante Reg ca SEM ee rp
16. 0mm step 0 1 mm see also fig 8 at end of 81 8 0 1 is the highest needle position 15 0 is the lowest needle position Bottom Wash Vertical Position is the height of the aspirating needle during the dispensing sequence of the bottom wash ASP DOWNW SPEED Aspiration Downward Speed 010 9 step 1 speed mini 0 speed 9 This Speed is applied only at the first Aspiration sequence DISP UPW SPEED Dispensing Upward Speed 010 9 step 1 speed mini 0 speed max 9 Dispensing Upward Speed is applied at the last Dispensing sequen ce BOT DOWNW SPEED Bottom Downward Speed 010 9 step 1 speed mini 0 speed max 9 Speed of all downward movements for all bottom sequences wash and aspiration BOT UPWARD SPEED Bottom Upward Speed 010 9 step 1 speed mini 0 speed 9 Speed of all upward movements for all bottom sequences wash and aspiration User Manual code 87050 1 16 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Cod 4 1 FEATURES AND SPECIFICATIONS 18 Software parameters for Wash protocols continued 1 8 8 Parameter s definitions continued a Bottom aspiration BOTTOM ASP following sequences are applied Downward aspirating movement of the needle into the well down to Aspiration Vertical Position Aspiration at Aspiration Vertical Position during Aspiration Time One or two upward aspirating movements up to Bottom aspiration
17. 2L RUBBER CAP LONG TUBE PW4x 12 CHANNEL MANIFOLD PW4x 8 CHANNEL MANIFOLD PW4x MANIFOLD CONNECTOR PW4x MANIFOLD CONNECTOR RINGS PW4x RUBBER SEAL FOR MANIFOLD PW41 2x12 CHANNEL MANIFOLD PW41 2x8 CHANNEL MANIFOLD PW41 3x8 CHANNEL MANIFOLD PW41 MANIFOLD CONNECTOR VACUUM SENSING PW40 LP35 COMPLETE TRAP VIAL PW40 LP35 TRAP VIAL CAP TUBE PW40 LP35 TRAP VIAL WITHOUT CAP PW41 MOTOR SELECTOR TUBING SET PW4x LP35 WASTE TUBING RED amp YELLOW PW4x DISPENSING TUBE BLUE PW4x EXTERNAL TUBING SET PW4x PINCH VALVE TUBE PW4x DISPENSING TUBE RINSE TRANSPARENT PW41 DISPENSING TUBE WASH2 WHITE PW41 DISPENSING TUBE WASH3 GREEN PW41 4 WAY VALVE TUBE PW41 LINK TUBE 4WAY VALVE UNIT PW41 SET TUBE MULTI WASH SELECTOR PW4x REMOVABLE uPLATE CARRIER PW4x HOLDER BOTTLE TOP WASHER PW41 HOLDER 2L BOTTLES BESIDE WASHER PW41 FUSE 2 5AT 5X20 250V PWAX LP35 HYDROPHOBIC FILTER PW41 85232 CORD PW40 STANDARD MAINTENANCE KIT PW MAINTENANCE KIT PW USER MANUAL F E Version July 1998 User Manual code 87050 PIN lt OS PW41 Oodes 86499 6 86608 Piate Washers Seno 99 051 05 Pasteur User Manual ppendix 72 09 96 HEVISION DATE 2 40 zs go z MARS EE EE EE See ee loma sois Sp Sg EE E E 7In0001 LOL EJ
18. Crosswise Aspiration T Bottom Aspiration vertical position Aspiration Time Bottom Wash Vertical Position GE Dispensed VOLUME d COVERELON HR ______ _ LIQUID E Vertical Speed Einem Aspiration Speed In aer Dispensing Speed Out BE uem iB Bottom Wash Speed In B ASP NUMBER j Bottom Wash Speed Out E Nr OF CYCLES LE END OF KIT NO oH poeseos Asseenon 81 8 3 Parametere definitions Crosswise Aspiration L KIT and PLATE parameters directly de pending on KIT such as dispensing as END OF KIT YES CL pirating times liquids etc are called KIT Parameters NR OF RII Parameters depending on microplate KITINTERVa _____ RENNES dimensions mainly POSITION and SPEED parameters are called PLATE parameters At programming each kit is mandatory linked to one plate with the Plate number parameter Fig 15 This implies that the PLATE must be already existing or must be pro grammed prior the associated KIT is programmed Up to 10 PLATEs can be programmed Up to 75 KITs can be programmed Version July 98 User Manual code 87050 3 2 UC Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 2 Access to PROGRAMMING mode Enter programming mode press simultaneously IN and OUT keys
19. Position followed by downward aspirating movement down to Aspira tion Vertical Position Aspiration at Aspiration Vertical Position during Aspiration Time In case of crosswise aspiration for flat bottom only every aspiration sequence close to the wall is immediately followed by an other aspiration sequence to the opposite end of wall as descri bed on page 1 10 Programmed parameters Kit parameters CROSW ASP Crosswise aspiration YES or NO for flat bottom only See details page 1 10 ASP TIME Aspiration time 0 1 to 9 9 sec step 0 1 sec See details page 1 10 BOT ASP NUMBER Bottom Aspiration Number 1 or2 Number of Bottom Aspiration Occurence Plate parameters ASP POS Aspiration Horizontal Position for flat bottom only 0 0 to 2 0 mm step 0 1 mm see also fig 8 at the end of 1 8 Aspiration Horizontal Position is applied for all Aspiration Bottom Wash and Bottom Aspiration sequences ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of 81 8 0 1 is the highest needle position 15 0 is the lowest needle position Aspiration Vertical Position is applied for all Aspiration sequences mra a M P M Version July 1998 147 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 855 FEAT
20. SHOCK Furthermore the electrical line must be protected by an adapted HAZARD fuse and a differential circuit breaker The access to the mains supply input plug located on instru ment rear panel must be kept free in order to use this plug if necessary as cut off device Although this instrument is fully insulated and grounded it is impor tant for all users to be aware of the potential hazards of using liquids in close proximity to an electrical supply In the event of any spillage the instrument must be immediately isolated from the electrical sup ply and drained Spilt liquid should be removed as soon as possible Serious damage to the instrument may occur if fluid is allowed to penetrate the case The electrical supply MUST NOT be reconnected until the instrument has been fully inspected by a qualified technician or service engineer SERVICING MUST ONLY BE CARRIED OUT BY A QUALIFIED TECHNICIAN OR SERVICE ENGINEER WARNING In some operating modes e g dispensing fluids or purging the sys SPILLAGES tem particularly under automatic control it is possible to overfill the wells of a microplate This can result in spillage of liquids and or con tamination with hazardous substances Care should be taken to en sure that overfilling does not occur If environmentally hazardous fluid is spilled the instrument must first be isolated from the electrical supply and physically isolated from the working environment by placing it in a
21. and dispenses a wash solution in the well When the dispensed volume is higher than well capacity the aspira tion needle aspirates the overflow to prevent liquid spilling on next wells Programmed parameters Kit parameters VOLUME for one well 50 to 3000 liL step 50 lil _ the well volume is approx 370 VL OVERFLOW 1 0 to 12 9 mm step 0 1 mm see also fig 8 the end of 81 8 This parameter sets the height of the aspirating needle and thus the height of the liquid in the well 1 0 is the highest needle position 12 9 is the lowest needle position for the overflow It is recommended to dispense a volume slightly higher than wished and have an overflow phase in order to obtain an equal distribution of volume in each well the slight flow rate variations among dispensing needles are compensated by the overflow phase LIQUID Wash W1 to Wash W9 When launching a kit in the RUN mode the display will remind you to connect correct Wash bottle prior starting FLOW flow rate compensation 5 to 5 step 1 The flow rate of the dispensing pump is adapted to the operating manifold However the flow rate can be slightly modified with this pa rameter lower flow rate with negative value higher flow rate with positive value Plate parameters DISP UPW SPEED Dispensing Upward Speed 0 to 9 step 1 speed mini 0 speed max 9 113 User Manual code 87050 Sanofi Diagnostics Pasteur Plate
22. is full How to restore good washing conditions ASPiration VERTical Position must be touching bottom of well to avoid aspirating needles blocked by foam well s wall Use CROSswise ASPiration Empty Waste Bottle prior starting to Wash Hydrophobic Filter is clogged Contact your application specialist for too strong too weak Flow Rate is not set correctly not enough quantity REVISION 03 Comments Flate parameter Aspiration Vertical Position Hydrophobic Filter Trap vial Waste bottle spensing Parameters Adjust FLOW rate compensation between 5 weak and 5 strong Volume is not set correctly not the same volume in every well FETT IT Often less volume in 1st strip than in the next ones Manifold dripping in rinsing tank when on standby before wash process Linzeoo Manifold dripping during Wash process One or several dispensing needles must be cleaned Overflow position not set correctly High concentration of wetting agent in wash buffer Rubber stopper in manifold dispensing chamber not set correctly This might create an excessive air bubble inside the dispensing chamber of manifold leading to dripping Manifold is dirty If still dripping Manifold connector with O rings is leaking Kit parameter Adjust VOLUME between 50 to 3000 uL by step of 50 iL Always dispense
23. pinch valve system old device 5 jGo to page 3 10 INPUT 1 SOL WASHR1 98 3 9 User Manual code 87050 Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual 0 4 3e OPERATING INSTRUCTIONS 3 6 Configuration continued 3 6 2 Washer s configuration continued EPOR ERE N continuation from page 3 9 41 only Activation of the vacuum GROUP T VACUUM DET YESESC detection Use T et 4 keys to set the vacuum sensing threshold Default value 20 points VAC T TRSH 4 20pts YESESC VACUUM DET ON A YES Use T and 4 keys to activate t 4 YESESC YES or deactivate NO vacuum detection Setting of RS232 serial port for connection to PC 5232 PARAM Baud rate can be reduced in case of transmission problem between Use T and 4 keys to set Baud T 4 Activation of the liquid detec tion in wash and waste bottles GROUP DET YESESC LIQUID WASH LEVEL DET YES Use T and 4 keys to activate t YESESC YES or deactivate NO liquid level detector in Wash bottle then in Waste bottle WASTE LEVEL DET YES T YESESC Setting of dialogue interface parameters Use and 4 keys to set language then key and T and 4 keys to activate Y or deactivate N
24. the Beep 4 t Version July 98 User Manual code 87050 3 10 CC Plate Washers PW40 amp PW41 Sanofi Diagnostics Pasteur User Manual _________ Codes 85499 amp 85508 3e OPERATING INSTRUCTIONS 3 6 Configuration continued 3 6 2 Washer s configuration continued LA C Re 8 continuation from page 3 10 Activation of a mini priming se quence before every wash pro tocol Use T and keys to activate Y or deactivate N then gt key and to setthe volume from 1 to 30ml Activation of the secured stop after every wash protocol to re move air from manifold Use T and keys to activate Y or deactivate N then gt key and TT and to setthe volume from 1 to 30ml Back to main menu See 83 3 ee Version July 98 3 11 User Manual code 87050 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 OPERATING INSTRUCTION 3 7 PROGRAMMING 3 7 1 Kit Structure F RES Au ROGRANME Parameters depending on Kit reagents A SEH AIC KIT2 re 1 Tkn PLATE uw a e ag Piate Name hu E Aspiration Horizontal Position Ges sme ome METHOD OO He M Aspiration verica Posion n
25. 40 amp PW41 Codes 65499 amp 855 OPERATING INSTRUCTIONS 3 2 Microplate loading Place the 96 well microplate on the plate carrier with 1 corner resting against metal spring on the right side fig 13 A1 corner Fig 13 a a Version July 98 User Manual code 87050 3 2 Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual 4 3 OPERATING INSTRUCTIONS 3 3 Select one KIT SE LECT RUN t I IN T 4 RUN KIT 75 t l Use T and 4 keys to select last strip to be processed for PW40 only This message is displayed when the requested wash buffer is not connected Once you have connected correct WASH bottle press YES and the pump will start to prime in order to operate with correct WASH liquid for PW41 See 21 on next page PRIMING ESC RUN 75 Summary of the selected items STRIP Kit name Number of strip YESESC Once the kit is launched the on going method is displayed on lower display line Press ESC to stop running kit Kit is terminated Display is back to previous kit selection B See 88 4 1 may appear as CONNECT THE WASH R9 Version July 98 4 3 User Manual code 87050 i Sanofi Diagnostics Pasteur User Manual Plate Washers PW40 amp PW41 Codes 85499 amp 85508 OPERATING INSTRUCTIONS 3 3 Select one KIT continu
26. 5499 amp 85508 3e OPERATING INSTRUCTIONS 3 7 PROGRAMMING continued 3 7 7 EDIT continued 3 7 7 2 EDIT PLATE Select plate to be edited PLATE PLATE 10 T YEs Eet Every parameter of the selected plate can be modified by using 4 keys ress YES to validate main pa meters modification 3 1 VALID MODI FIC ATI o J T YES SELECT RUN t 4 IN YES 01 T A Back to main menu See 3 3 Version July 98 User Manual code 87050 3 29 Plate Washers PW40 amp PW41 Sanof Diagnostics Pasteur o __ Codes 85499 amp 85508 3e OPERATING INSTRUCTION User Manual 3 7 PROGRAMMING continued 3 7 8 COPY 3 7 8 1 COPY KIT Select kit to be copied Select kit before which you want to place copy Enter copy name select charac ter with T and I keys then press gt key to move to next cha racter up to 15 characters 3 7 8 2 ERROR on KIT copy No kit programmed ERR 27 NO KIT You must first add a kit PROGRAMMED See also ERR 24 ERR 25 ERR 32 SELECT RUN on 83 7 4 2 EE 3 30 Version July 98 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual odes 85499 amp 85508 3 OPERATING INSTRUCTIONS 3 7 PROGRAMMING continued 3 7 8 COPY continued 3 7 8 8 COPY PLATE PLATE 05 Select plate to be copied Selec
27. 558 9 6658 sepoo jgnuew sasn LfrMd OfrMd SJOMSBM 4 so sou6ela ijoues oodes 85199 6 80508 Plate Washers PIN 0 s nof eiegnos jos User Manual IER CIRE CL Ge pope que LIRE Gei te NK AL EURE en sou s cpmepee PO RO GO REES mJ seqwowpwopwA ow m0 ee eT T eo S C t USINE LE Ls Lee 9 om TE LE e SE Ee s LEE wees ET CN rm oms Loss li hrs ove ss oae zo LRA RE tert EL onem Lug trc eios m ms E RE M 101 euinjoA pesuedsig GOH L3N 5 vg T lt LIX JeAJ91U H3 LNI 3NI L axvus WOLLOG uagWnN
28. 8 BOT WASH NUMBER 1 16 BOT WASH ASP 1 10 Bottom Aspiration Vertical Position 1 18 BOTTOM ASP 1 17 1 18 Bottom Downward Speed 1 16 Bottom shape 3 19 BOTTOM TIME 1 16 Bottom Upward Speed 1 16 Bottom Wash Aspiration 1 10 Bottom Wash Vertical Position 1 16 BOTTOM WASH 1 15 Bottom Washing Bottom Aspiration 1 9 1 10 Centering 3 19 Clean manifold 4 2 Compensation 1 13 Contents 2 COPY KIT 3 30 Crosswise aspiration 1 12 CROSW ASP 1 12 CWA 1 12 D Daily Maintenance 4 1 DELETE 3 25 DELETE KIT 3 25 DELETE METHOD 3 25 DELETE PLATE 3 26 Disinfection 4 3 DISP UPW SPEED 1 13 DISPENSING 1 13 Dispensing Upward Speed 1 13 E EDIT KIT 3 27 EDIT METHOD 3 28 EDIT PLATE 3 29 Electrical Data 1 7 Electric Shock Hazard 5 End of Edit 3 27 ERR 24 3 16 ERR 25 3 16 3 18 26 3 22 27 3 30 28 3 31 29 3 26 ERR 32 3 76 ERR 33 3 22 ERROR on METHOD programming 3 18 ERROR on PLATE deletion 3 26 ERRORS on KIT programming 3 16 ERRORS on PLATE programming 3 22 F FLOW 1 13 Flow rate compensation 1 13 H Hardware specifications 1 7 Hardware version 4 4 HORIZONTAL SPEED 3 20 Hydrophobic filter 1 3 IN 3 1 INSERT 3 23 INSERT KIT 3 23 INSERT METHOD 3 23 INSERT PLATE 3 24 K Keyboard 3 1 KIT Parameter 1 12 Kit Structure 3 12 L LIQUID 1 13 Liquid level detection in bottle 1 6 Version July 1998 User Manual code 87050 A2
29. Alternating current Direct and alternating current Earth ground terminal D Protective earth ground terminal Version July 1998 User Manual code 87050 Caution see enclosed documents Diagnostics Pasteur Plate Washers PW40 amp PW41 UserManud 7 Codes 85499 amp 85508 ENSURE that all connections are tight and all blocking elements foams have been removed before switching on the Washer If there is any doubt or concern about the safety of the instrument contact the nearest approved service centre Version July 1998 7 User Manual code 87050 Sanon Diagnostics Pasteur Plate Washers amp PW41 ser Manual Codes 85 99 amp 85508 de FEATURES AND SPECIFICATIONS PW40 11 PW40 Basic features i 2x20 characters LCD Display and 5 soft Keys Aerosol Protection Cover Wash bottle Rinse bottle Waste bottle Manifold 8 Channel or 12 Removable 8 Mode 12 Mode Plate Carrier Removable Fig 1 Autoclavable at 120 250 Version July 1998 User Manual code 87050 1 1 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 8B508 FEATURES AND SPECIFICATIONS PWAO 12 PW40 Outside dimensions mm Washers height 243 mm without bottles Fig 2 Version July 1998 User Manual code 87050 1 2 Sanofi Diagnostics Pasteur User Manual Plate Washers
30. E You have entered no name or the IMPOSSIBLE YES plate name you have entered is already existing You are promp ted to enter a new name to conti nue plate programming M M A Q m9 rr M HH Version July 98 User Manual code 87050 3 22 Plate Washers PW40 amp PW41 ee Pasteur shore ate 3e OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 5 INSERT 3 7 5 1 INSERT KIT The new kit is inserted before another kit INSERT gt KIT Select kit before which you want to insert new kit BEF KIT Nr23 Enter new name Then same procedure as in 3 7 4 1 add Nr24 NAME KIT i gt 3 7 5 2 TEMPORARY TEMPORARY KIT 4 YESESC Power failure or erroneous OFF switching during KIT insertion will cause the kit to be lost unless the first method has been completely programmed In this case the completely programmed methods can be found in a kit named TEMPORARY KIT This kit is always inser ted at the first place of the kit list It is then possible to COPY this Temporary Kit and write its correct name then continue with program ming of next methods and kit repetition parameters Once saved under its correct name it is highly recommended to DELE TE the TEMPORARY KIT 3 7 5 3 INSERT METHOD The new method is inserted before another method INSERT METHOD i YES ESC KIT KIT Nr23 YESESC METHOD DI
31. EED Aspiration downward ASP DOWNW SPEED Dispensing upward speed DISP UPW SPEED Bottom Wash downward speed BOT DOWNW SPEED Bottom Wash upward speed BOT UPWARD SPEED SHAKING AMPLITUDE St OddS NOISIA3U 96 60 JU 31 3294 xjpueddv 809S8 66fr98 sapoo lenueirj jasn I Md 3 OMW SI8USEM 91814 mesed sonsouBeig yours ees 05028 9 02 ENUEN 225 2 iie 866 UoJS48A 01 OL 10 14 70008 PN LOL 01 01 OL 1008 SN LOL NOOO N E ES toi Clemes en 101 10 581 ie LO teld 10 ges 252 LOL Kit name NAME NAME PLATE 190001 1 211008 SN LOL SOLS SNL 11009 ON LOL 101813 17008 101 10008 N 10 29110008 CN Lo wooe 17008 CN 10005 10006 EN LOL 11006 28 CN 7000 ZN LOL 17008 iN LOL 77008 LN LOL o e T 5 5 5 2 o 16 9 lt Crosswise Aspiration 222 8 ASP Aspiration time 1711222222112 55 Un PE LEE El ET Er EE a EL Elus E Overflow position 0223222202022 Selected liquid d 5 LIQUID Bottom Wash Number Bottom Wash Time 2200202222022 0220200102 nn HEED EEE ED BOT ASP NUMBER Method Interval MET INTER Ca se mos sco ww xlpu9dclv 80
32. ERFLOW 1 010 12 9 mm step 0 1 mm 1 0 is the highest needle position 12 9 is the lowest needle position for the overflow Overflow is applied at the last Dispensing sequence See details page 1 11 LIQUID Wash W1 to Wash W9 See details page 1 11 FLOW flow rate compensation 5 to 5 step 1 See details page 1 11 Version July 1998 115 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 85508 FEATURES AND SPECIFICATIONS 18 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued w Bottom Washing continued BOTTOM BOT WASH NUMBER Bottom Wash Number WASH 2 continued Number of Bottom Wash Occurence BOTTOM TIME Bottom Wash Time 0 1 to 9 9 sec step 0 1 sec Time when dispensing at the Bottom Wash Vertical Position Plate parameters ASP POS Aspiration Horizontal Position for flat bottom only 0 0 to 2 0 mm step 0 1 mm see also fig 8 at the end of 51 8 Aspiration Horizontal Position is applied for all Aspiration Bottom Wash and Bottom Aspiration sequences ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of 81 8 0 1 is the highest needle position 15 0 is the lowest needle position Aspiration Vertical Position is applied for all Aspiration sequences B W VERT POS Bottom Wash Vertical Position 0 1 to 15
33. ES to validate main pa MODIFICATION 9 vati rameters modification VALID T i Press T keys until end of edit message PARAMETERS YESESC EDIT MAIN t 4 EDIT END OF EDIT Press YES to quit EDIT mode t 4 YESESC Press T keys to go backto Parameters or Method SELECT RUN T 4 Q Back to main menu See 3 3 Version July 98 User Manual code 87050 3 27 Plate Washers PW40 amp PW41 Cod 499 amp 85 INSTRUCTIONS Diagnostics Pasteur User Manual OPERATING 3 7 PROGRAMMING continued 3 7 7 EDIT continued 3 7 7 1 EDIT KIT continued O A continuation from page 3 27 EDIT METHOD Select method to be edited Then every parameter of the method can be modified by using T or 4 keys Note that the liquid Wash Wx cannot be modified VAL ID MODI FI Ci T CON Press YES to validate method ESC modification Press T or keys until end of edit message EDIT METHOD t I OF Press YES to quit EDIT mode Press T or keys to go back to Main Parameters or Method EDIT END t i SELECT RUN a Back to main menu See 3 3 C Back to kit edition See 83 7 7 1 Version sJulv 9B eS Version July 98 3 28 User Manual code 87050 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 8
34. H Memory is full because one or se YES veral kits are containing a large number of methods You must first delete an existing SELECT RUN kit prior programming a new one t I IN _ M H M a Version July 98 User Manual code 87050 3 16 METHOD KIT 4 MET INTER T i METHOD WASH T VOLUME 4 SOAKING SELECT RUN T i IN Version July 98 User Manual code 87050 3 7 PROGRAMMING continued 3 7 4ADD continued ADD METHOD OMN YESESC Plate Washers PW40 amp PW41 Sanofi Diagnostics Pasteur User Manual Codes 85499 amp 85508 3 OPERATING INSTRUCTIONS The method is added after the last method of the selected kit Use T and keys to scroll the available menu Press YES key to initiate the dis played selection Press ESC key to escape and go back to former prompt Select kitto which you wantto add a method with t and i keys Set Method interval between last existing method and added me thod with T or 4 keys from Omn O amp up to 59mn Os Set kit parameters associated to selected method See table next page and kit para meter definition 1 8 3 Only the first cycle is played n times here twice for a two cycle method such as Wash Asp nW 4 A 3 17 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 3 OPERATING
35. INSTRUCTIONS 37 PROGRAMMING continued 3 7 4ADD continued 3 7 4 8 ADD METHOD continued Summary of kit parameters associated to method d S x 5 5 i nW4A WASH ASP nw a lB WASH ASP DI 9ENSING Method Code Value See Kit parameter d VOLUME OVERFLOW 12 9ram WASH Wl to WASH W9 FLOW 5 to 5 0 1 t NUMBER 9 9 1 to Nr OF CYCLES SOAKING 0 to 59 mn in PLATE mode 0 to 9 9 sec i RIP mode 3 7 4 4 ERROR on METHOD programming ERR 25 NOT ENOUGH Memory is full because one or se MEMORY YES veral kits are containing a large number of methods You must first delete an existing kit or existing method prior programming a new one SELECT RUN t bi IN YES Version July 98 User Manual code 87050 3 18 Diagnostics Pasteur Plate Washers PW40 amp PW41 Codes 8S499 amp 85508 User Manual Ge OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 4 ADD continued 3 7 45 ADD PLATE The plate is added at the end of the existing plate list Use T and keys to scroll the available menu Press YES key to initiate the dis played selection Press ESC key to escape and go back to former prompt PLATE YES ESC NAME PLATE 10 Enter plate name select charac T I YESESC ter with T and 4 keys then press
36. SPENSING Enter new method name Then same procedure as in 83 7 4 3 add method Select kit where a method has to be inserted Select method before which the new method has to be inserted Version July 98 User Manual code 87050 3 23 Plate Washers PW40 amp PW41 Codes 85499 amp 85508 3e OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 5 INSERT continued 3 7 54 INSERT PLATE The new plate is inserted before another plate Sanofi Diagnostics Pasteur User Manual INSERT PLATE t i YES E5 C Select plate before which the new 01 plate has to be inserted YES PLATE BEF PIATE 00 Enter new method name i YES ESC Then same procedure in para 3 7 4 5 add plate t Version July 98 User Manual code 87050 3 24 Plate Washers PW40 amp PW41 Deler eege bi Codes 85499 amp 85508 OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 6 DELETE 3 7 6 1 DELETE KIT DELETE KIT YESESC Select kit to be deleted Press YES key to confirm kit is deleted or press ESC key to es cape SE LE CT RUN T i IN YES OUT 3 7 6 2 DELETE METHOD DELETE METHOD Select kit to which you want to de lete a method Select method to be deleted Press YES key to confirm method is deleted or press ESC key to escape METHOD ASPIRATION Note deleti
37. See 84 3 1 Hardware and Software version Version July 98 User Manual code 87050 3 8 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 3 OPERATING INSTRUCTIONS 3 6 Configuration continued 3 6 2 Washer s configuration Enter CONFIGURATION menu SELECT CONFIGURATION Selection of Manifold i Ld GROUP MANIFOLD T YESES C MANIFOLD A 2 8 Use T and 4 keys to set 7 4 YESE type according to installed mani fold PW40 8 channel manifold ow PW41 2 8 channel manifold o PW 41 only Assignment of Wash amp Rinse inputs on the rear panel GROUP VALVE t PARAM Use T and 4 keys to select the input number INPUT SELECT T I 1 Use T and 4 keys to assign wash W1 to W9 or R1 to R9 or rinse solution to selected input Note input 4 is mandatory as signed to Rinse INPUT T 1 4 SOL WASH Wl GROUP RINSE PARAM T 4 Setting of countdown prior to launching automatic rinsing AUTORINSEIN Use T and 1 keys to set count t 4 down minutes from 0 to 240 minutes Automatic Rinse is deactivated when Rinse Time is set at O Selection of the multiwash selector type on rear panel GROUP VALVE TYPE YESES C MOTOR Default setting is MOTOR YESESC Use T and 4 keys to select PINCH if the washer is equipped with a 4
38. TIONS 37 PROGRAMMING continued 3 7 10 RAM initialization Every programmed kit and programmed plate are saved on a RAM Random Access Memory The contents of this MEMORY can be erased by a RAM initializa tion thus deleting the whole set of kits and plates that have been programmed by the user Instrument software and DEMO kits and plates are not deleted as they are saved in an EPROM not affected by RAM initialization How to perform the RAM initialization Turn the unit ON while keeping those keys pressed SELECT RUN Following message is displayed RAM INITIALIZATION Press YES to have the RAM reset SELECT RUN t 1 IN Version July 98 User Manual code 87050 3 33 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 4 MAINTENANCE TROUBLESHOOTING 4 1 Routine maintenance Procedures 4 1 1 Daily Maintenance Prior starting to wash manifold are not clogged It is recommended to pour Otherwise change pinch valve appropriate disinfection solution tube clean manifold see S 4 2 1 in the bottom ofthe WASTE bot hereafter tle prior connecting it to the washer Thus the infected liquid When washing is terminated coming from the washer and go Rinse the washer Use only ing into the WASTE bottle will be deionized water for rinsing Turn deactivated the washer off Tubes and mani IMPORTANT Avoid bleach fold will be kept we
39. URES AND SPECIFICATI 18 Software parameters for Wash protocols continued 1 8 8 Parameter s definitions continued a Bottom aspiration continued BOTTOMASP Plate parameters continued continued BOT VERT POS Bottom Aspiration Vertical Position 0 1 to 15 0mm step 0 1mm see also fig 8 at the end of 1 8 0 1 is the highest needle position 15 0 is the lowest needle position Bottom Aspiration Vertical Position is the height of the aspirating needle during the aspiration sequence of the bottom aspiration ASP DOWNW SPEED Aspiration Downward Speed 010 9 step 1 speed mini 0 speed 9 Aspiration Downward Speed is applied atthe first Aspiration sequen ce BOT DOWNW SPEED Bottom Downward Speed 010 9 step 1 speed mini 0 speed max 9 Speed of all downward movements for all bottom sequences wash and aspiration BOT UPWARD SPEED Bottom Upward Speed 010 9 step 1 speed mini 0 speed max 9 Speed of all upward movements for all bottom sequences wash and aspiration n i Version July 1998 User Manual code 87050 118 C P Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 FEATURES AND SPECIFICATIONS 18 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued Ag AGITATION EI Version July 1998 User Manual code 87050 Plate Agitation Th
40. Washers PW40 amp PW41 User Manual Codes 89499 amp 8SS0B 1 FEATURES AND SPECIFICATIONS 18 Software parameters for Wash protocols continued 1 8 8 Parameter s definitions continued WASH Aspiration sequence followed by Dispensing sequence Programmed parameters Kit parameters CROSW ASP Crosswise aspiration YES or NO for flat bottom only See details 1 10 ASP TIME Aspiration time 0 1 to 9 9 sec step 0 1 sec See details page 1 10 VOLUME for one well 50 to 3000 nl step 50 liL the well volume is approx 370 IxL OVERFLOW 10 12 9 mm step 0 1 mm 1 0 is the highest needle position 12 9 is the lowest needle position for the overflow See details page 1 11 LIQUID Wash W1 to Wash W9 See details page 1 11 FLOW flow rate compensation 5 to 5 step 1 See details page 1 11 Plate parameters ASP POS Aspiration Horizontal Position for flat bottom only 0 0 to 2 0 mm step 0 1 mm see also fig 8 at the end of 1 8 ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of 1 8 0 1 is the highest needle position 15 0 is the lowest needle position ASP DOWNW SPEED Aspiration Downward Speed 0 to 9 step 1 speed mini 0 speed max 9 DISP UPW SPEED Dispensing Upward Speed O to 9 step 1 speed mini 2 0 speed max 9 Version July 1998 User Manual code 87050 114
41. ce cursor on strip with gt key then press key to have strip omitted X In RUN mode the omitted strips will never be processed Select first method of kit See method parameter selection on 3 7 4 3 METHOD WASH ASP Answer NO in order to add an other method INTER 0 MN45 5 Set Method interval with T or i from Omn Os up to 59mn Os METHOD DISPENSING T Answer YES when no more methods have to be added Gp to page 3 16 Version July 98 User Manual code 87050 3 15 Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual 4 3 OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 4 ADD continued 3 7 4 1 ADD KIT continued en eee UT TIAM continuation from page 3 15 Nr OF KITS Select the Nr of times the kit has T i to be run with T or J keys up to 9 times KIT INTER OMN OS When run more than once set Kit t i gt YESESC interval with T or I keys from Omn Os up to 59mn59s The new kit is added at the end of the existing kit list Display is back to main menu 3 7 42 ERRORS on KIT programming TOO MANY 75 kits are already programmed You must first delete an existing kit prior programming a new one You have entered no name or the kit name you have entered is alrea dy existing You are prompted to enter a new name to continue kit programming NOT ENOUG
42. e microplate is shaken horizontally during the Agitation T mewith an Agitation Amplitude and an Agitation Speed Programmed parameters Kit parameters SHAKE TIME Agitation time 0 1 to 59 9 sec step 0 1 sec Plate parameters SHAKING AMPLITUDE Agitation Amplitude 0109 step 1 SHAKING SPEED Agitation speed 0109 step 1 119 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 1 FEATURES AND SPECIFICATI 18 Software parameters for Wash protocols continued 1 8 8 Parameter s definitions continued Dispensing Needle 0 HOME POSITION Identification and range of para meters related to the location of the needle in relation to the mi 1 croplate well r 2 Overflow Position 1 010 129 Kit parameter Microplate Well 6 7 8 Aspirating Needle 9 Aspiration 10 Vertical Position Bottom 11 Aspiration Vertical Position Bottom Wash 12 rtical Positi 4 0 1 10 15 0 Aspiration Horizontal Position Plate parameters 0 1 to 2 0 mm 13 Plate parameter 14 1 15 Version July 1998 User Manual code 87050 1 20 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 2e INSTALLATION 2 4 Unpacking Carefully unpack accessories and washer IMPORTANT Do not lift up the washer b
43. ed ERR 21 with PW44 SELECT RUN 1 IN RUN KIT 01 RUN KIT 75 t i LAST STRIP I i ERR 21 WASH CONNECTED SELECT RUN T 1 IN YESOUT Error 21 indicates that Wash buffer W9 or R9 requested in KIT 75 is not connected Connect Wash buffer W9 or R9 at input 1 2 or 3 Then enter washer configuration to modify input assignment ac cordingly see 83 6 2 may appear as 21 WASH R9 NOT CONNECTED D 3 4 Version July 98 User Manual code 87050 CU UC Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 3 OPERATING INSTRUCTIONS 3 4 WASH interruption or Power Failure 3 4 1 WASH interruption When the ESC key is pressed whereas a kit is running the follo wing message is displayed STOP THE TEST The elapsed time since the kit has 0H 3mnl7s RES ESC been stopped is displayed Press the RES key to resume the kit at the point where it has been stopped or press the ESC key to go back to main menu RUN KIT75 RUN KIT 75 YESESC A1 Back to main menu See 83 3 3 4 2 POWER Failure When a power failure occurs whereas a kit is running the following message will be displayed when power is back POWER FAILURE The elapsed time since the kit has been stopped is displayed Press the RES key to resume the kit at the point where
44. ersion July 1998 tin User Manual code 87050 4 d PUMP PRIMING RUN SELECT Plate Washers PW40 amp Codes 85499 amp 85508 4 MAINTENANCE amp TROUBLESHOOTING 4 3 Troubleshooting 4 3 1 Hardware and software version Diagnostics Pasteur User Manual SERVICE VER SELECT T T and I keys are deactivated on this prompt VERSIONS YESESC GROUP t VERSION 2 2 Software version of the washer YESES Default User Data version Kit list and Plate list programmed in factory saved on EPROM Default user data are not deleted by a RAM initialization R Back to main menu See 83 3 Version July 1998 4 4 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 Appendix Accessories and spare part list Part Number Designation PWAX LP35 WASTE BOTTLE 2L W O CAP PWAX LP35 COMPLETE WASTE BOTTLE 2L PWAX LP35 WASTE 2L RUBBER CAP TUBE PW4x COMPLETE WASH BOTTLE PW4x RINSE 2L SCREW CAP TUBE PW4x WASH BOTTLE WITHOUT CAP PW40 WASH 2L SCREW CAP TUBE PW4x RINSE BOTTLE WITHOUT CAP PW4x COMPLETE RINSE BOTTLE PW41 COMPLETE WASTE BOTTLE DETECTOR PW41 SCREW CAP WASH BOTTLE LEVEL DETECTION PW41 COMPLETE WASH BOTTLE TUBE LEVEL DETECTION PW41 COMPLETE WASTE BOTTLE 8L LEVEL DETECTION PW41 WASTE
45. fume cupboard or similar loca tion Contact the nearest service centre for assistance advising them of the health hazards associated with the spilled materials WARNING OPERATING THIS EQUIMENT IN WAYS OTHER THAN DETAILED INCORRECT 5 MANUAL MAY IMPAIR THE PROTECTION PROVIDED BY OPERATION INSTRUMENT DO NOT operate this equipment with the covers removed poten tially dangerous voltages are contained within DO NOT operate this equipment with the safety earth ground dis connected CAUTION DO NOT poke fingers pencils or other implements into plate carrier entry slot Version July 1998 5 User Manual code 87050 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 SAFETY INFORMATION SAFETY SYMBOLS BE PREPARED for unexpected movement of the plate carrier and noise from the Washer pump when the Washer is controlled by exter nal computer ENSURE that the main power cable is correctly wired Colour codes are as follows Europe United States BROWN LIVE BLACK LIVE BLUE NEUTRAL WHITE NEUTRAL GREEN YELLOW EARTH Ground GREEN GROUND The following alarm and information symbols can be found in several places in the washer Only a qualified personal aware of the security procedures is allowed to repair this instrument Please read this manual carefully before using this instrument Direct current gt Ac z
46. g Mo senonolganosaospas eul User Manual I D Loi 9008 ELL 71008 N LLL 31V d 3INVN duis G O3INVIN Mgnue oode 82050 Version 1 Logg 7 050 8 apoo sn DN 8661 YASA Bottom shape 5 SHAPE Aspiration horizontal position POS Plate centering La CENTERING Aspiration vertical p position ASP VERT POS Bottom aspiration fe vertical position BOT VERT POS Bottom Wash 10 vertical position B W VERT POS un Aspiration downward speed ASP DOWNW SPEED Dispensing upward speed DISP UPW SPEED Bottom Wash upward BOT UPWARD SPEED HOddS NOISIASU xipuecldv must aiva 19995 Eid 80558 9 66158 sepoo lenuBIN Jssn jnajsed so sou6eia IJOUBS ifrMd 8 OfrMd SJegsBM a Bld 31 05 96 Bad washing condition Too much residual volume in the wells One needle forgets temporarily to aspirate spensing Factor generating risks Aspiration Vertical Position not set correctly Aspiration Horizontal Position not set cor rectly Aspiration Downward Speed not set correctly High concentration of wetting agent in wash buffer needle might be blocked by foam Waste bottle
47. ins socket washer is equipped with universal input switch mode power supply 85 VAC to 264 VAC 47 Hz to 440 Hz Turn the washer ON ON OFF switch on rear panel Whereas plate carrier and manifold are moving to their home posi tion following message appears on display After initialization SELECT RUN VERSION 2 xxx 2Y PW41 A a a A Fill RINSE bottle with demineralized water and prime hy draulic circuit with at least 5 RINSE operations see RINSE mode 83 5 The washer is ready to use Version July 98 2 2 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 3 OPERATING INSTRUCTIONS 3 1 Run Mode 3 1 1 How to use the Keyboard VERSION 2 Xxx 2Y PWA41 Press OUT Key to set or remove microplate plate carrier moves out and is locked for 10 se conds to allow micropla te to be placed in carrier Press T or i Keysto scroll main menu See para 3 6 1 Main Menu page 3 8 Press YES Key to enter selected mode Press IN key to move Plate Carrier In The function of the key is displayed on the screen Depending on displayed menu some keys are activated some others have no function and are therefore deactivated Version July 98 3 1 User Manual code 87050 Sanofi Diagnostics Pasteur User Manual Plate Washers PW
48. it has been stopped or press the ESC key to go back to main menu A Back to main menu See 63 3 Version July 98 3 5 87050 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85S06 OPERATING INSTRUCTIONS 3 5 RINSE 3 5 1 RINSE with PW40 SELECT PRIME RINSE t 4 IN YESOUT Connect blue tube on RINSE bottle ensure the plate carrier is not removed then press YES CONNECT ON THE RINSE A YESESC PRIMING RINSING ESC SELECT RUN t 3 5 2 Please RINSE with PW40 The message PLEASE RINSE YES is displayed to incite user to perform a RINSE operation when the washer has not been used during 10 minutes after a WASH opera tion Press YES Key to go back to main menu and launch a RINSE IMPORTANT The washer should never be switched off with WASH solution inside the tubing and manifold Solid crystals from dried WASH solution could clog the dispense need les and clamp electrovalve tube Version July 98 3 6 User Manual code 87050 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 550 3 OPERATING INSTRUCTIONS 3 5 RINSE continued 3 5 8 RINSE with PW41 PRIME RINSE IN YESOUT SELECT T 1 Way is mandatory A PW41 Press YES to continue SELECT WAY A Selec
49. lied n times n 1 to 9 For method using two elementary cycles only the first cycle is ap plied n time When repeated several times the waiting time between elementary cycles is called Soaking Time The waiting time between two methods is called Method Interval Whatever the selected mode STRIP or PLATE see definition be low a method is always completely finished on the whole plate prior starting the next method Available methods Method Abbreviated Name Method Name on LCD Display Single cycle Method nw WASH Wash nA ASPIRATION Aspiration nD DISPENSING Dispensing nw BOTTOM WASH Bottom Washing na BOTTOM ASP Bottom aspiration nAg AGITATION Plate Agitation Two cycle Method nW A WASH Wash Aspiration nW a WASH BOT ASP Wash Bottom Aspiration nw BOT WASH ASP Bottom Wash Aspiration nw a B WASH B ASP Bottom Washing Bottom Aspiration Kit parameters associated to method STRIP or PLATE PLATE mode the elementary cycle is applied successively on all strips prior applying next elementary cycle STRIP mode the total method is applied on the strip prior proces sing the next strip STRIP Mode Method 3W Aon 1st strip e whole plate 3W A 3W A on 2nd strip W on the whole plate etc W on the whole plate 3W A on last strip A on the whole plate _ Version July 1998 User Manual code 87050 110 ee GH EC Sa
50. mmable Each kit is saved under its own name up to 15 characters and is a combination of methods repeated or not with or without soaking times in strip mode or in plate mode Method 6 single cycle washing methods Wash Aspiration Dispensing Bottom Washing Bottom Aspiration Agitation 4 two cycle washing methods Wash Aspiration Wash Bottom Aspi ration Bottom Wash Aspiration Bottom Wash Bottom Aspiration Soak time 0109 9 sec Strip mode 0 to 59 min 59 sec in Plate mode Repetition Every method can be repeated from 1 up to 9 times Wash mode Strip mode or Plate mode Omit strip Strips can be omitted at kit programmation Plates Up to 10 plate parameter sets program mable Accepts flat or curved bottom microplates Vertical and horizontal speeds vertical and horizontal positions of the aspirating nee dle in relation with the wells are program mable Other features Priming sequence of the hydraulic system at change of Wash solution Disinfection program of the hydraulic cir cuit User programming can be inhibited Version July 1998 User Manual code 87050 1 8 UC Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 1 FEATURES AND SPECIFICATIONS 1 8 Software parameters for Wash protocols This section describes the Wash protocols their methods and their software parameters It is useful to be read prior sta
51. ng the method of a one method kit is equivalent to delete this kit Version July 98 User Manual code 87050 3 25 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 8 3e OPERATING _ INSTRUCTION 37 PROGRAMMING continued 3 7 6 DELETE continued 3 7 6 3 DELETE PLATE DELETE PLATE T i Select plate to be deleted PLATE PLATE 10 Press YES key to confirm plate is deleted or press ESC key to escape SELECT RUN t I 3 7 6 4 ERROR on PLATE deletion ERR 29 PLATE ASSOC the plate is still associated with one TO A KIT YES or several kits You must first associate an other plate to affected kits or delete tho SELECT RUN se kits prior deleting this plate T MMM MM P Version July 98 User Manual code 87050 3 26 User Manual Codes 85499 3 OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 7 EDIT 3 7 7 1 X EDIT KIT KIT Select kit to be edited zI Select MAIN PARAMETERS or METHOD with T and 1 keys EDIT MAIN EDIT METHOD T 4 t 4 _ YESESC jGo to page 3 28 Edit main parameters Every displayed parameter can be changed by using T or I keys Example the plate associated to this kit can be changed PLATE t 4 MANIFOLD 8 T 4 STRIP 1 X 3 t KIT INTER OMN 05 Press Y
52. nofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85 2 FEATURES AND SPECIFICATIONS 18 Software parameters for Wash protocols continued 1 8 2 Methods continued Nr OF CYCLES Number of Cycles 2 n 1 to 9 step 1 number of elementary cycles that will be applied within the method SOAKING Soak time Omn Osec to 59mn Osec step 1 sec in PLATE mode Omn Osec to 9 9sec step 0 1 sec in STRIP mode Waiting time between two consecutive elementary cycle within one method MET INTER Method Interval Omn Osec to 59mn Osec step 1 sec Waiting time between two consecutive methods Version July 1998 User Manual code 87050 1 11 Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 8550 FEATURES AND SPECIFICATIONS 18 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions Parameters directly depending on KIT or Wash protocol such as dis pensing aspirating times liquids etc are called KIT Parameters Parameters depending on the microplate dimensions mainly POSI TION and SPEED parameters are called PLATE parameters In the washer the elementary cycles are as follows A ASPIRATION aspirating needle goes down and aspirates until reaching the well bottom The needle is shifted close to wall Aspiration Horizontal Position for flat bottom well h it remains centered for curved bottom well
53. ote A back to main menu from page 3 9 8 3 Operating Instructions Upgrade according to the configuration of the vacuum sensing adjustable vacuum threshold Modified page page 3 10 6 3 6 2 Washer s configuration Tie blocking the manifold on the left side is still used Modified page page 2 2 52 Installation Upgrade according to the new PW40 trap vial Yellow tube is now transparent and orange tube is now yellow Modified page 1 3 1 Features and Specifications Update of the accessories and spare part list Modified page 8 Appendix Version July 1998 User Manual code 87050 Sanofi Diagnostics Pasteur User Plate Washers t PW40 amp PW41 _ Codes 85499 amp 85508 Appendix Index Symbols 2 8 Channel 1 4 4 liquid inputs 1 4 8 Channel 1 1 A Access to PROGRAMMING 3 13 ADD KIT 3 15 ADD METHOD 3 17 ADD PLATE 3 19 Aerosol Protection Cover 1 1 1 4 AGITATION 1 19 Agitation Amplitude 1 19 Agitation speed 1 19 Agitation time 1 19 ASP DOWNW SPEED 1 12 ASP HOR POS 1 12 ASP TIME 1 12 ASP VERT POS 1 12 ASPIRATION 1 12 Aspiration Downward Speed 1 12 Aspiration Horizontal Position 1 12 Aspiration time 1 12 Aspiration Vertical Position 1 12 Autoclavable 1 1 1 4 B B W VERT POS 1 16 B WASH B ASP 1 9 1 10 Beep 3 10 BOT ASP NUMBER 1 17 BOT DOWNW SPEED 1 16 BOT SHAPE 3 19 BOT UPWARD SPEED 1 16 BOT VERT POS 1 1
54. peed max 9 Vertical speed Up Down manifold speed during non work movements movement to home position etc Oto 9 step 1 speed mini 0 speed max 9 Aspiration downward speed See definition on para 1 8 3 Version July 98 User Manual code 87050 3 20 CC UC Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 4 User Manual 3 OPERATING INSTRUCTIONS 3 7 PROGRAMMING continued 3 7 4 ADD continued 3 7 4 5 ADD PLATE continued dp 54 continuation from page 3 20 DISP UPW SPEED 6 t i YESESC Dispensing upward speed See definition on para 1 8 3 BOT SPEED 3 Bottom downward speed YESESC See definition on para 1 8 3 Bottom upward speed UPWARD SPEED See definition on para 1 8 3 YES BOT SHAKING AMPLITUDE 2 Shaking amplitude t YESESC See definition on para 1 8 8 Shaking speed See definition on para 1 8 3 SHAKING SPEED E YESESC SEL ECT RUN t 4 IN YESOUT Version July 98 3 21 User Manual code 87050 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Cod 508 3e OPERATING INSTRUCTIONS 3 7 PROGRAMMING continued 3 7 4ADD continued 3 7 4 6 ERRORS on PLATE programming ERR 26 ALREADY 10 plates are already program YES med You must first delete an ex isting plate prior programming a d new one SELECT RUN t 4 IN YES OUT ERR 33 PLAT
55. rting to programme new wash protocols or new kits or Kit 1 Method 1 nW Method 2 Method 3 nA Wash protocol 1 1 8 1 WASH protocol definition A WASH protocol also called a KIT is made of a succession of Wash Methods There are 10 different wash methods available and every method is programmed with its own batch of specific parameters Also the Wash protocol is associated to a batch of plate param eters Positions that are set in respect to plate geometry flat U or V shaped well as well as Speeds needed for the wash process Plate 10 Positions and Speeds Im S Wash protocol 75 Plate 1 Positions and Speeds The available methods are Method Method Name Single cycle Method nw Wash nA Aspiration nD Dispensing nw Bottom Washing na Bottom aspiration nAg Plate Agitation Two cycle Method Fig 7 nW A Wash Aspiration nW a Wash Bottom Aspiration nw A Bottom Wash Aspiration nw a Bottom Washing Bottom Aspiration Version July 1998 User Manual code 87050 19 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 8 85508 1 FEATURES AND SPECIFICATION 18 Software parameters for Wash protocols continued 1 8 2 Methods A method is a completely defined module made of one or two ele mentary cycles Every kit is made of a succession of methods In a method elementary cycles can be app
56. rush sup outside of the dispense and as plied in maintenance kit thor pirate needles oughly clean the small dispense Remove the silicon top seal of chamber Thoroughly rinse dis the manifold pense chamber with deionized Using the big pin supplied in water Ensure that all impurities maintenance kit clean the big as removed from the manifold pirating needles Plug dispense chamber of the Rinse out the aspirate chamber manifold with the silicon plug with deionized or distilled water Place it as close as possible to Thoroughly clean the walls ofthe the first needle however without aspirating cavity blocking it Extra silicon caps are e Look through the aspirate nee supplied the maintenance kit dles to ensure that cleaning is ad Remount the manifold replace equate the tubing and reprime Put the silicon top seal back in its place User Manual code 87050 4 2 Diagnostics Pasteur Plate Washers PW40 amp PW41 r Manual Codes 85499 amp 85S08 4 MAINTENANCE TROUBLESHOOTING 4 2 Cleaning and disinfection continued 4 2 2 Disinfection The disinfection program applies to internal surfaces of tubing connec tors manifold and manifold needles SELECT DISIN FECTION T i IN YESOUT Use disinfection solution approved by your laboratory Connect the tube s on rinse bottle cap Fill RINSE bottle with the disinfection solution Ensure the plate carrier is not
57. sion October 1996 July 1997 September 1997 April 1998 July 1998 Appendix Upgrade Description First official release of the user manual compatible for both PW40 and PW41 washers Implementation of the new motorized multiwash selector Modified pages page 1 6 5 1 6 LP41 Hydraulic circuit page 3 9 3 6 2 Washer s Configuration Implementation of the selection between the new multiwash selector MOTOR and the old one PINCH valve Page 3 10 modified accordingly and page 3 11 created Implementation of the new internal design with integrated blocking device of the manifold Modified pages page 2 2 8 2 Installation Modified warning message for electrical shock hazard Modified pages page 5 Bottles are removed from top and placed beside the washer Modified pages page 1 1 1 2 1 4 1 5 1 7 6 1 Features and Specifications page 2 1 8 2 1 Unpacking Tie blocking the manifold on the left side is replaced by adhesive tape reason the vertical guide is shorter Implemented in serial product in May 98 Modified pages page 2 2 8 2 Installation WASH may appear as WASH on display in several firmware versions In the manual the affected displays are marked with an and the alternative message is displayed on a foot note The text associated to the displays mention both Wx or Rx possibilities Modified pages page 3 3 3 4 amp 3 9 3 11 transfer of foot n
58. slightly more than the awaited volume in the well and aspirate the volume in excess by setting the OVERFLOW po sition see below Always ensure dispensing needles are clean Use metallic cleaning pin supplied in maintenance kit Adjust OVERFLOW position of the aspirating needle in order to aspirate the excess of dispensed VOLUME Respect dilution factor Never use higher wetting agent concentration than required dimension for Rubber Stopper mm square 23 lt AS24 0 Kit parameter Kit parameter dispensing needles Cleaning Set supplied in Maintenance Kit Activate MiniPrime and Secured Stop to compensate and minimize dripping Change pinch Valve tube Ensure O rings are correctly seated in manifold connector If still leaking change O rings Contact your application specialist for advice Protect aspirating needles with adhesive tape Spray silicone grease on dispensing needle ensure all the outside surface of needle is treated Clean inside of dispensing needles with metallic pin supplied maintenance kit In Main menu select CONFIGURATION available in firmware equal or more recent than 1 50 2Y for PW40 and spare supplied in Maintenance kit spare supplied in Maintenance kit 3 eo gt E 9 66458 5 Ord Plate Washers PW40 amp PW41 Sanofi Diagnostics Pasteur User Manual Codes 8549 Revi
59. t plate before which you want to place copy PLATE 05 I Enter copy name select charac ter with T 1and I keys then press 4 key to move to next cha racter up to 8 characters NAME PLATE 04 t 4 t 3 7 8 4 ERROR on PLATE copy ERR 28 NO PLATE No plate programmed PROGRAMMED YES You must first add a plate See also ERR 26 ERR 33 3 7 4 6 SELECT RUN 5 T i IN Version July 98 3 31 User Manual code 87050 Sanofl Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 OPERATING INSTRUCTIONS 37 PROGRAMMING continued 3 7 9 Plate Test Step by Step RUN The programmed kit can be used in a step by step mode i e manifold and plate carrier are stopped at each programmed positions Aspira tion horizontal position Aspiration vertical position Overflow position etc in order to check if these positions are correct If not correct user must edit see 3 7 7 the tested kit or its assigned plate in order to modify position parameter s Use keys to select kit to be tested Press YES key to move to next programmed position Keep the YES key pressed for a continuous motion preferably deactive the beep The kit is processed on the whole plate Version July 98 User Manual code 87050 3 32 Sanofi Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Code 4 3 OPERATING INSTRUC
60. t washer input according to rinsing or priming that you want to do Indication of the liquid connected to selected input If you change liq uid use T or keys to modify input assignment same as in washer configuration page 3 9 Note Input 4 is mandatory RINSE INPUT 4 SOL RINSE t YESESC PRIMING RINSING SELECT RUN t 3 5 4 Automatic Rinsing with PW41 PRIMING RINSING One automatic rinsing sequence will take place as soon as the time set in RINSE PARAM see 3 6 2 Washer s configuration has elapsed after the last wash Note When the washer won t be used for several days it is recommended to connect all inputs to rinse bottle and rinse every input as described above in order to clean the pinch valve tube of every input See also 4 1 2 Storage condition IMPORTANT The washer should never be switched off with WASH solution inside tubing and manifold Solid crystals from dried WASH solution could clog the dispense need les and clamp electrovalve tube Version July 98 3 7 User Manual code 87050 Sanof Diagnostics Pasteur Plate Washers PW40 amp PW41 Codes 85499 amp 85508 User Manual OPERATING INSTRUCTIONS 3 6 Configuration 3 6 1 Main menu RUN T i See 83 1 Run Mode SELECT PRI ME RI Ng PRIME RINSE N YES OUT See 83 5 RINSE SELECT DISINFECTION t See 84 2 2 DISINFECTION CONFIGURATION See 83 6 2 Washer s configuration SERVICE VER
61. t with water Fill the rinse bottle with deionized that will prevent them from clog water Connect it to the washer ging Launch a Rinsing sequence and Empty WASH bottle rinse itthor check that pinch valve tube and oughly with deionized water 4 1 2 Washer storage conditions The washer should never be further dispensing of liquid stored with Wash solution inside this case priming must be dispensing tubes and manifold made with the help of a syringe otherwise the liquid pump the filled with deionized water con pinch valve tube and the mani nected at the Rinse input of the fold might be clamped if com washer pletely dry thus preventing any Version July 1998 User Manual code 87050 4 1 Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 amp 85508 4 MAINTENANCE amp TROUBLESHOOTING 4 2 Cleaning and disinfection 4 2 1 Cleaning the manifold The manifold is infectious Dispense needle and chamber Disinfect it prior any further Push the silicon cap from the cleaning chamber of the manifold using a See disinfection procedure on stem or a screwdriver entered next 4 2 2 from the opposite end of the cy lindrical chamber Aspirate needles and chamber Using one of the small pin sup Remove the manifold plied in maintenance kit clean Using a duster or a scratch the small dispense needle ing utensil thoroughly clean the Using the cylindrical b
62. y pulling on the foam frames First remove accessories at the top and beside the washer e Then hold the washer not the foams and lift up Remove the foams remove plastic bag and place washer on a stable horizontal surface Install bottles beside the washer as shown in user manual on 81 1 for PW40 or 1 4 for PW41 Fig 10 Version July 98 2 1 User Manual code 87050 Sanof Diagnostics Pasteur Plate Washers PW40 amp PW41 User Manual Codes 85499 08 2 INSTALLATION 2 1 Unpacking continued In washer working area Fig 11 12 amp 13 Remove adhesive tape blocking aerosol cover Remove adhesive tape blocking plate carrier to bottom plate fig 11 Pull and push plate carrier to check that it moves freely Manifold Remove the tie blocking the manifold on the left side fig 12 push the tie to the left Remove the tie from the vertical shaft Turn blocking device as shown on fig 12 and ensure to not hinder the manifold Up Down motion On rear panel Connect tubes on rear panel match colours blue Wash 1 gray Wash2 green Wash3 transparent Rinse red Waste bottle liquid Yellow Vacuum to Waste bottle air Vacuum pump protection Cut yellow tube at halfway between WASTE bottle and washer and place hydrophobic filter supplied in maintenance kit Connect power cord Fuse installed 2 5 A Slow Blow Plug Power Cord to ma

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