CVP-2 - User manual
Contents
1. CED Medical Biological Research amp Technologies CVP 2 Centrifuge Vortex for PCR plates for version V 1AW Operating manual Certificate Contents 1 salei Precautions ss sin cto jacceincenct a aaa 4 2 General INO AL Ola sa asirai asis as a ais i da arai a a is a i beets 6 2 Genng Shae ecg xc hee cart E E R 6 4 So 2162 hee caer ener eee err ce ere ee a S 8 5 SPeciCaON Serine as e aa ia ai a a a a a a a a 12 6 How to choose a proper adapter cccccseececeeeeecceeeeecceeseeceeueeeceeeeessaaeees 13 7 Mamntenante sse a E eee ee 13 8 Warranty and claimS 23020 eet ee a 14 9 Declaration Of CONTORMINY Ls ais ias a aa i ase ai a 15 1 Safety Precautions The following symbols mean AN Caution Make sure you have fully read and understood the present A Manual before using the equipment Please pay special attention to sections marked by this symbol GENERAL SAFETY This unit is a state of the art piece of equipment which is extremely safe to operate Nevertheless it may lead to danger for users or others nearby if used by untrained staff in an inappropriate way or for purposes other than those it was designed for Save the unit from shocks or falling Store and transport the unit in a horizontal position see package label After transportation or storage keep the unit under room temperature for 2 3hrs before connecting to electric circuit Before using any cleaning or decontamina2. I position on the rear press the Open key and open the lid by lifting it upwards by hand unscrew the fixation nut Fig 1 1 counter clockwise using the wrench included in standard set and remove the nut place the rotor and secure it tightly with the fixation nut placing the nut with the key holes up Fig 1 1 and turning it clockwise by the included wrench place the safety cover Fig 1 2 on the rotor by pressing the cover clip Fig 1 3 down close the centrifuge lid Note To close the lid press down the middle part of the edge of the lid until the clicking sound is heard Otherwise the lid will not be secured properly which will cause operation malfunction turn off the centrifuge mn switch on the cet position O Aft Fig 1 Rotor replacement 4 Operation Recommendations during operation 4 1 4 2 4 3 44 Do not use rotors with visible signs of corrosion wear or mechanical damage Check the rotor buckets for any signs of wear or corrosion and replace if necessary When using semi skirted or non skirted PCR plates put the plate into an appropriate adapter Insert the plate into the rotor with the adapter Refer to section 6 How to choose a proper adapter Load both PCR plates to balance the unit during operation Before operation check PCR plates and ensure that they are closed properly Content can be spilled from open PCR plates endangering personnel working with hazardous materials
3. For proper mixing it is recommended to fill PCR plates not more than 75 of their nominal volume Seal the microplates with an appropriate adhesive film to avoid spilling the sample 7 6 82 CVP 2 SPIN MIX Centrifuge RPM 1200 300 biosan Vortex for Time 20 00 4 sec PCR plates Cycles Mode RUN 10 3 4 5 9 1 Fig 2 Front panel Connect the external power supply to a properly grounded power socket Turn on the power switch I position on the rear side of the unit Lid unlocks automatically Press the Open key fig 2 1 and open the lid by lifting it upwards by hand indication OPEN will appear in the Mode bottom line fig 2 2 Lid will open only with rotor stopped Remove the safety cover by lifting the safety cover clip Insert TWO microtest plates in rotor one opposite another 4 5 4 6 4 7 4 7 1 4 7 2 4 7 3 4 7 4 4 8 4 8 1 4 8 2 4 8 3 4 8 3 4 8 4 4 8 5 Place the safety cover on the rotor and secure it by pressing the safety cover clip down tightly Close the outer lid carefully and smoothly until the clicking sound is heard indication STOP will appear in the Mode bottom line Note To close the lid press down the middle part of the edge of the lid until the clicking sound is heard Otherwise the lid will not be secured properly which will cause operation malfunction Quick centrifugation Press the QS fig 2 10 button for quick mixing sedimentation and h
4. damage Warranty covers only the units transported in the original package 3 2 Standart set Package contents Standard set TGVP 2 Centrifuge VorteXx asalasasa dia a a as 1 pce R 2MP rotor for PCR plates with fixation nut for fastening And Salet COVEN assist asai ii an ai di sa i lates a i ar ae E 1 pce AP 96 adapter for semi skirted and non skirted PCR plates 96 wells cccccee 2 pcs wrench for rotor removing O ccceccccseececceseeecceeeeceeeeesseseesnaneeeseaes 1 pce key for cover unblocking fixed into rear panel of the unit lt 1 pce a External Power SUDDIV cise aides i i asai i a a 1 pce oje gezo S 1 pce Operating manual Certificate cc ceccccccccscsssseeeeeeeeesaeeeeeeeeeessaaeeeees 1 copy Optional accessories AP 384 6 adapter 384 well PCR plates Eppendorf 0 on request mu e Setup place the unit on the even stable horizontal and clean surface remove protective film from the display plug the power cord into the external power supply plug the external supply into the power socket on the rear of the unit and posi tion the unit so that there is easy access to the power switch and mains outlet according to EN 61010 2 20 people and hazardous materials must not be within a 300 mm area around the device during the centrifuge operation Rotor installation connect the power cord to a properly grounded mains outlet Switch on the power switch
5. user is responsible for decontaminate the unit before its decommissioning and utilization 2 General Information After many years of Combined Centrifuge Vortex concept success we are proud to introduce the long awaited Centrifuge Vortex for PCR plates CVP 2 to the sample preparation market The application range of CVP 2 is PCR plates with skirts semi skirts or without them The Spin mix spin technology is intended to spin down micro volumes of reagents on the well s bottom first centrifugation spin following mixing mix and spin down the reagents again second spin We named this repetitive algorithm sms spin mix spin It aims at reducing the mistakes during sample preparation for PCR analysis The algorithm was published for the first time and patented by Biol Dr V Bankovskis V Bankovskis et al Riga Latvia Pat No P94 74 CVP 2 is a fully automatic device for reproducing sms for 2 PCR plates at the same time thus saving time considerably A must have instrument for PCR and DNA analyses laboratory CVP 2 is 4 devices combined in 1 Centrifuge Maximum RCF 245 x g 1500 rpm Vortex regulation timer 0 60 s Centrifuge Vortex SMS for realization of the sms algorithm PED 5 3 Getting started 3 1 Unpacking Remove packing materials carefully and retain them for future shipment or storage of the unit Examine the unit carefully for any damage incurred during transit The warranty does not cover in transit
6. 11 2 Set the required vortexing speed from 300 to 1200 rpm increment 100 rpm fig 6 2 4 11 3 Set the required vortexing time from 1 to 60 s increment 1 s fig 6 3 1 23 CVP 2 SPIN MIX Centrifuge OFF 300 Vortex for off 10 sec PCR plates MIX STOP Fig 6 Vortexing 4 12 Press Run key fig 2 5 to start operation 4 13 Rotor will begin rotating and on the display indication RUN fig 2 2 cycle countdown fig 2 6 and changing parameters of the current mode fig 2 7 and fig 2 8 4 14 Operation can be stopped before the set number of cycles elapses if necessary by pressing the Stop key fig 2 9 4 15 Unit stops automatically after executing set number of cycles blinking indication STOP fig 2 2 on the display and the lid unlocks automatically A sound signal is emitted after full stop of the rotor Press Stop key fig 2 9 to stop the signal 4 16 At the end of operation set the power switch in position O OFF on the rear Disconnect the external power supply from electric circuit Note The electrical lid lock allows opening the lid only when the unit is connected to the mains and is turned on 4 17 Lid emergency opening Disconnect the power cord from external power supply unit Wait until the rotor stops completely Insert the provided lid unblocking key found on the rear panel of the unit into opening on the right side of the unit and press to unlock the lid 5 Specifications The unit is de
7. fting it upwards by hand to open an unpowered unit insert the provided lid unblocking key Screwed in the rear panel of the unit into opening on the right side of the unit and press open the rotor safety cover by lifting the cover clip by hand fig 1 3 holding the rotor with one hand unscrew rotor fixation nut turning it counterclockwise using included wrench retrieve the rotor and perform necessary cleaning The rotor is autoclavable 120 C 20 min after cleaning place the rotor and secure it tightly with the fixation nut placing the nut with the key holes up and turning it clockwise by securing wrench place the rotor safety cover on the rotor pressing the cover clip down tightly 13 8 1 8 2 8 3 8 4 8 5 Warranty and claims The Manufacturer guarantees the compliance of unit with the requirements of Specifications provided the Customer follows the operation storage and transportation instructions The warranted service life of unit from date of delivery to the Customer is 24 months Contact your local distributor to check availability of extended warranty Warranty covers only the units transported in the original package If any manufacturing defects are discovered by the Customer an unsatisfactory equipment claim shall be compiled certified and sent to the local distributor address Please visit www biosan lv Technical support section to obtain the claim form The following informat
8. ion will be required in the event that warranty or post warranty service comes necessary Complete the table below and retain for your records Centrifuge Vortex for PCR plates CVP 2 Serial number Date of sale O 14 9 Declaration of conformity Declaration of Conformity Equipment name CVP 2 Type of equipment Centrifuge Vortex for PCR plates Directive EMC Directive 2014 30 EC Low Voltage Directive 2014 35 EC RoHS 2011 65 EC WEEE 2002 96 EC amp 2012 19 EU Manufacturer SIA BIOSAN Ratsupites 7 build 2 Riga LV 1067 Latvia Applied Standards EN 61326 1 Electrical equipment for measurement control and laboratory use EMC requirements General requirements EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN 61010 2 20 Particular requirements for laboratory centrifuges We declare that this product conforms to the requirements of the above Directive s A e LA Ak HA Signature Signature Svetlana Bankovska Aleksandr Shevchik Managing director Engineer of R amp D Date Date 15 Biosan SIA Ratsupites 7 build 2 Riga LV 1067 Latvia Phone 371 67426137 Fax 371 67428101 http www biosan lv Edition 1 01 May 2015
9. iosan Centrifuge RPM 1200 300 Vortex for Time 5 00 10 sec PCR plates Cycles Mode M S STOP Fig 3 Sms algorithm 4 9 Centrifugation vortexing 4 9 1 Select the alternating centrifugation and vortexing algorithm indication M S of Mode parameter fig 4 1 4 9 2 Set the required centrifugation speed from 300 to 1500 rpm increment 100 rpm fig 4 2 4 9 3 Set the required centrifugation time from 1 s to 30 min increment 1 s after 1 min 1 min fig 4 3 4 9 3 Set the required vortexing speed from 300 to 1200 rpm increment 100 rpm fig 4 4 4 9 4 Set the required vortexing time from 1 to 60 s increment 1 S fig 4 5 4 9 5 Set the required sms algorithm cycle count from 1 to 999 fig 3 6 1 2 3 45 CVP 2 SPIN MIX biosan Centrifuge RPM 1200 300 Vortex for Time 5 00 10 sec PCR plates Cycles Mode S STOP Fig 4 Centrifugation vortexing 4 10 Centrifugation 4 10 1 Select the centrifugation mode indication SPIN of Mode parameter fig 5 1 4 10 2 Set the required centrifugation speed from 300 to 1500 rpm increment 100 rpm fig 5 2 4 10 3 Set the required centrifugation time from 1 s to 30 min increment 1 s after 1 min 1 min fig 5 8 1 2 3 CVP 2 SPIN MIX Centrifuge RPM 1200 OFF Vortex for Time 5 00 off PCR plates Cycles Mode SPIN STOP Fig 5 Centrifugation 10 4 11 Vortexing 4 11 1 Select the vortexing mode indication MIX of Mode parameter fig 6 1 4
10. old it pressed for the desired time The unit will automatically when the QS button is released Setting parameters By pressing Select key fig 2 3 choose a parameter to change Each pressing of the Select key consecutively activates parameters in operation cycle currently active parameter is indicated by blinking Use VY and A keys fig 2 4 for setting the corresponding values pressing a key for longer than 2 seconds will increase change rate Speed RPM and spinning mixing time Time can be changed during operation when a new operation cycle starts microprocessor automatically accept changes as current operation mode Cycle count Cycle and operating mode Mode cannot be changed during operation Mode fig 3 1 parameter can be one of the following S M S sms algorithm mode M S alternating centrifugation and vortexing SPIN centrifugation only MIX vortexing only Sms algorithm Select the sms algorithm indication S M S of Mode parameter fig 3 1 Set the reguired centrifugation speed from 300 to 1500 rpm increment 100 rom fig 3 2 Set the required centrifugation time from 1 s to 30 min increment 1 s after 1 min 1 min fig 3 3 Set the required vortexing speed from 300 to 1200 rpm increment 100 rpm fig 3 4 Set the required vortexing time from 1 to 60 s increment 1 S fig 3 5 Set the required sms algorithm cycle count from 1 to 999 fig 3 6 1 2 3 4 5 CVP 2 SPIN MIX b
11. or PCR plates with a fixator lid BS 010219 AK AP 96 Set of 2 adapters for semiskirted and unskirted BS 010219 DK 96 well PCR plates AP 384 Set of 2 adapters for 384 well PCR plates BS 010219 EK Eppendorf Biosan is committed to a continuous program of improvement and reserves the right to alter design and specifications of the equipment without additional notice gt Accurate within 10 12 6 How to choose a proper adapter 6 1 Displayed in the table below are PCR plate types in relation to available adapters Effective mixing Do not use Piate tvoe No AP 96 AP 384 yP adapter adapter adapter HC S 96 well skirted PCR plate Piko PCR plates in frame 4 pcs frame 96 well semi skirted PCR plate 96 well non skirted PCR plate 384 well PCR plate Eppendorf AN Warning PCR plate height inside an adapter should not be higher than 17 mm 7 Maintenance 7 1 If the unit requires maintenance disconnect the unit from electric circuit and contact Biosan or your local Biosan representative 7 2 Only qualified and specially trained personnel must perform all maintenance and repair operations 7 3 Standard ethanol 75 or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and disinfection of the unit Follow these steps for cleaning the rotor to open a powered unit press Open key fig 3 1 and open the outer lid li
12. signed for operation in cold rooms incubators and closed laboratory rooms at ambient temperature from 4 C to 40 C in a non condensing atmosphere and maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Speed setting range Centrifugation Ee Soaccaaseaaldens loadaaaeeogeane Saas 300 1500 rpm N ONT OXIING aa S 300 1200 rpm Speed setting INCFEMEN cceeeeeeeeecceeceeeeeeeeeeeeeeeseeeeeeeeeeeeseaeeeees 100 rpm Relative centrifugal force cccsssceccssseeceeeeeeceeeeeeseeeesseeeeeens up to 245 xg Centrifugation mode time range cccccceeseeeeeeeeeeeeaeeeeeeeeeeeeaaes 1s 30 min Centrifugation mode time increment 00ccceeeee 1 s after 1 min 1 min Vortex Mode time LANGE L is ins amiaus i an ais 0 60s Vortex mode time INCreMent ccccccceeececceeceeceeeeeeceeeeeeseeeeseaeeeeseneeeeas 1s Number of programmable Cycles ccccssseeesseeeeeseeeeeeeeeeeens 1 999 cycles Maximum DIETS ICIOING Ee aa eis 17 mm Bo ry rer eer ener et nee ne nner one ee eer en E LCD 64x128 px Sav au Lid with a lock DIMENSIONS TS 285x350x190 mm Vasil a S 6 15 kg Operating voltage power CONSUMPTION 0 ccceeeeeees 24 V 750 mA 18 W External power supply input in AC 100 240V 50 60 Hz out DC 24 V Optional paserbilon Catalogue accessories P number R 2MP Rotor f
13. tion methods except those recommended by the manufacturer check with the manufacturer that the proposed method will not damage the equipment Do not make modifications to the design of the unit ELECTRICAL SAFETY Connect only to the external power supply with voltage corresponding to that on the serial number label Use only the external power supply provided with this product Ensure that the external power supply is easily accessible during use Do not plug the unit into an ungrounded mains outlet and do not use an ungrounded extension lead Disconnect the unit from the electric circuit before moving Disconnect the external power supply from mains outlet to turn off the unit If liquid penetrates into the unit disconnect it from the external power supply and have it checked by a repair and maintenance technician Do not operate the unit in premises where condensation can form Operating conditions of the unit are defined in the Specifications section DURING OPERATION Do not use rotors with visible signs of corrosion wear or mechanical damage Do not use the unit without the rotor safety cover Do not operate the unit in environments with aggressive or explosive chemical mixtures Please contact manufacturer for possible operation of the unit in specific atmospheres Observe the safety area of 300 mm around the unit Persons and hazardous materials must not be located in the safety area whilst the unit is operating Do not cen
14. trifuge flammable or chemically active substances Do not use outside laboratory rooms Do not operate the unit if it is faulty or was installed incorrectly Always fix rotor securely Stop the operation immediately with the Stop key if any unusual noise occurs during acceleration which can be due to improper rotor fixation Use only original accessories rotors adapters etc provided by the manufacturer and ordered specifically for this model BIOLOGICAL SAFETY According to EN 61010 2 020 a centrifuge without biological safety system is not considered a biologically safe and therefore cannot be used for centrifuging hazardous materials contaminated with toxic radioactive or pathogenic microorganisms Take additional measures if a contact of bare skin with hazardous substances is possible Take care not to cut puncture or tear the gloves Not one glove material is impervious to all substances Disposable surgical or PVC gloves provide substantial but not complete protection PVC gloves are probably more protective than surgical gloves but they are stiffer and less tactile Discard gloves after each use It is the user s responsibility to carry out appropriate decontamination if hazardous material spills on or penetrates into the equipment Some inner components are susceptible to contamination Only qualified specialists who are experienced in deactivations procedures must perform cleaning these components for their future use The
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