Assay Blaster! User Manual Table of Contents
Contents
1. e Plate i 1S U HIDE Clear all wells Layout Analyte Name i 1 2 3 andard Wlode E Edit Select ternplate Concentration Background Unknown Made Hide Mode Figure 2 Plate Layout toolbar 3 1 Assigning Standards Turn on the Standards function by highlighting the Standards button At this point you should select whether you want to populate your standards by column down arrow or row right arrow Change your arrow Selection by clicking on the correct arrow Next select the number of standard replicates in your analysis by using the Standard Replicate drop down menu e g select 1 if only one well per standard was run select 2 if duplicate standard wells were run Select the wells you wish to populate as standards by clicking on a well and dragging your mouse to create the grid You may also double click on an individual well to either populate it or to de populate it The standard designation e g Standard 1 Standard 2 is determined by the direction of auto population as well as your number of standards A minimum of 6 and a maximum of 12 standards may be selected Once you have populated your standards the standard concentrations in the left margin under the Standards tab will become editable Figure 3 You may enter your standard concentrations either manually or by copying and pasting using the keyboard command Ctrl C and Ctrl V for PC or Cmd C and Cmd V for Mac2. Unknown Dillution Sample ID Unknown 1 j1 Unknown 2 Unknown 3 Unknown 4 Unknown 5 Unknown 6 Unknown 7 Unknown 8 yell ID Unknown 8 1 Unknowns Aoo o o y Unknowns Aoo o o Standarda ooo oo o y y J lalenan Z Dillution Sample ID nimia www enzolifesciences com Enzo Life Sciences i Strescoen Incorporating ALEXIS assay designs BIOMOL Stressgen Figure 6 Plate Layout Chart 3 6 Save plate layout You may save your plate layout at anytime by selecting the Save plate layout button Save the layout to your hard drive If you would like to recall a saved plate layout on the opening page select I would like to use an existing experiment template and find the saved file on your hard drive A saved layout will contain all your selections on the plate layout page including analyte name unit well designations standard concentrations dilutions of unknowns sample ID s Proceed to the next page by selecting the Proceed to Input Data button or by selecting the Input Data tab 4 Input Data The Input Data tab allows you to assign values to each designated well See Figure 7 to view the functions and buttons included on the Input Data toolbar You must enter a textual description for the Y axis label Proceed to Standard Curves Fa Aceny Blaster B Clear all wells all wells C O MERE eA ENEE ero the
3. ax b In the 4P logistic fit the application creates a curve using best curve fit y a d 1 x c b d If you choose 5P logistic fit the application creates a curve using best curve fit y a d 1 x c b g d You should choose the curve model which offers the best fit for your particular set of data You may save an image of the standard curve as a jpeg file by clicking the Save image as jpeg button from the upper toolbar To save all settings sample files used curve fitting parameters exclusion of data points from the analysis etc for later analysis you can click on the Export experiment data button on upper toolbar A pop up window will display experiment details up to this point Confirm with OK and then select the directory in which you would like to save the file A file including all settings will be generated in xml format You can import this saved setting file on the opening page Select I would like to use an existing experiment template and find the saved XML file on your hard drive A saved experiment will contain all parameters and selections up through the Standard Curves tab The three check boxes at the bottom left allow you to view statistical data about your 4P and 5P Logistic Fit graphs on the standard curve plot If you click on the Display equation on chart checkbox the equation will be displayed for that plot with actual values for that curve fit If you click on the D
4. EXT jOVR Figure 18 Standard curve graphs 17 7 5 Standard Data Table Displays concentration Y axis value and back calculated value for your standard curve Figure 18 Edit View Insert Format Tools Table Window Help Type a question For help X af OL 2 44 Normal unkne unknown mi2 Assay Blaster Standard Data Table Standard Values for Hsp27 5P Logistic fit Back Calculation Concentration in pg mL 1 000 96 299 89 129 64 Figure 19 Standard data table LIMITED WARRANTY Assay Designs Inc warrants that at the time of shipment this product is free from defects in materials and workmanship This warranty is in lieu of any other warranty expressed or implied including but not limited to any implied warranty of merchantability or fitness for a particular purpose Assay Designs must be notified of any breach of this warranty within 48 hours of receipt of the product No claim shall be honored if Assay Designs is not notified within this time period The sole and exclusive remedy of the customer for any liability based upon this warranty is limited to the replacement of the product or refund of the invoice price of the goods The Assay Blaster data analysis software is intended for research use only and is not for use in diagnostic applications 18 Enzo Life Sciences Inc Software License Agreement This Software License Agreement Agreement for the software you are installing Sof
5. label you wish to appear on ae Y Axis of the standard Curves graph Figure 7 Input Data 4 1 Assigning values to wells To assign values to the wells copy and paste your data using only keyboard commands from an excel spreadsheet All copied data within a 12x8 grid will be pasted in the display however only data within the designated standard and unknown wells will be used in analysis Y Axis Label MeanoD 9 eel ee ee eee www enzolifesciences com Enzo Lite Sciences Al Eyi assay designs BIOMOL Stressgeri Figure 8 Input Data 4 2 Hidden wells If your plate layout contains a hidden well you will not be able to assign a value to that well If you try to paste into a hidden well the value will be skipped see Figure 9 eray Blasen Enzo Y Axis Label MeanoD O O S MES EX EIESEVESERERESEDEAES www enzolifesciences com Te ae ALEXIS assay designs BIOMOL Stressgeri Figure 9 Input Data Hidden wells 4 3 Clear all wells If you wish to clear the plate of all file selections click the Clear all wells button A prompt will ask if you wish to proceed If you clear the values all selections are lost and cannot be recovered 4 4 Viewing well and file information You may view a chart of your well and file information in the bottom section of the window Figure 10 The File Selection chart includes information such as well assignment well ID dilution sample ID and signal valu
6. using Linear X Axis and Signal Y axis Immunometric j Asray Blaren Standard Curves Y Axis Hsp2 Standard Curve Signal 7 Curve fit Equation y 107 36 0 10 107 36 1 0 x 3401 44 1 0483542 0 0754457 5P Logistic Fit v R 2 1 000 ED50 3 401 41 X Axis andard year kate A Display equation on chart Display R 2 on chart V Display ED50 on chart 400 500 600 Concentration pg mL www enzolifesciences com Enzo Hile Selen ALEXIS assay designs BIOMOL Stressger Figure 15 Duplicate series 5P Logistic Fit plot using Linear X Axis and Signal Y axis Immunometric 12 B ecap Blaren Onz OOA Standard Curves Y Axis Serotonin Standard Curve Curve fit X Axis Linear X Axis C Display equation on chart C Display R42 on chart C Display ED50 on chart 90 100 110 120 130 140 150 160 170 180 190 200 21 Concentration ng mL www enzolifesciences com Enzo Life Sciences incorporating ALEXIS assay designs BIOMOL Stressger Figure 16 Duplicate series 5P Logistic Fit plot using Linear X Axis and B BO Y axis Competitive Peray blamen Standard Curves Plate Layout Y Axis Serotonin Standard Curve Curve fit 5P Logistic Fit M X Axis Input Data C Display equation on chart L Display R42 on chart _ Display ED50 on chart 20 30 Concentration ng mL www enzolifesciences com Enzo Life Sciences incorpor
7. ARTICULAR PURPOSE NOTWITHSTANDING LICENSOR REPRESENTS THAT THE SOFTWARE IS Y2K COMPLIANT LICENSEE ACKNOWLEDGES THAT THE LOADING OF THIS OR ANY THIRD PARTY SOFTWARE ON A COMPUTER SYSTEM MAY RESULT IN SYSTEM DAMAGE DATA LOSS DATA CORRUPTION OR SOFTWARE INCOMPATIBILITY Miscellaneous Licensee may not assign this Agreement or any rights or obligations hereunder directly or indirectly by operation of law or otherwise without the prior written consent of Licensor Subject to the foregoing this Agreement shall inure to the benefit of and be binding upon the parties and their respective successors and permitted assigns No waiver or modification of this Agreement shall be valid unless in writing signed by each party The waiver of a breach of any term hereof shall in no way be construed as a waiver of any other term or breach hereof If any provision of this Agreement is held to be unenforceable such provision shall be reformed only to the extent necessary to make it enforceable This Agreement shall be governed by Michigan law except for conflict of laws principles This Agreement contains the complete agreement between the parties with respect to the subject matter hereof and supersedes all prior or contemporaneous agreements or understandings whether oral or written All questions concerning this Agreement shall be directed to Enzo Life Sciences Inc Attn Research and Development 10 Executive Boulevard Farmingdale NY 11735 USA 19 www enzol
8. Experiment Select to either begin a new experiment or to use an existing experiment template To create a new experiment select the top radio button and select Enter You may also select an existing experiment template saved to your hard drive during a previous analysis To select an existing experiment template select the bottom radio button and hit the browse button Your navigation menu will open Find your desired experiment template on your hard drive and select Enter At this point the application will take you directly to the Plate Layout tab Eey Blaster I would like to start a new experiment I would like to use an existing experiment template www enzolifesciences com Enzo Life Sciences y Streeeoers Incorporating ALEXIS assay designs BIOMOL Stressgr n Figure 1 Selection of the experiment Plate Layout This page allows you to assign specific designations to your 96 well plate layout It also lets you alter your standard curve parameters See Figure 2 to view the functions and buttons included on the Standard Curve Data toolbar You may enlarge the chart or plate section of the window by clicking on the arrows in the border between the two sections Proceed to Input Data Eise Blaster Notes Help Goto Enzo Life Sciences website i 3 S Enzo 8 8 LALO Standards l Unknown 1 Standard Replicate Unknown Replicate
9. Hiding wells If you wish to leave a selected well out of analysis you may use the Hide function This allows a user to skip wells that they do not wish to use in analysis If a well is hidden the value assigned to that well will not be used in analysis To hide a well highlight the Hide well button and double click on the desired well Unknown Eai meno rene Y gt Mhd ERAS HIDE l Unknown 2 M Unknown 3 M Unknown 1 a OO Unknown ft J Unknown a Unknown 8 Hosay glistey Standard Curves www enzolifesciences com Enzo tie sciences ALEXIS assay designs BIOMOL Stressgeri Figure 5 Hiding wells 3 4 Clear all wells If you wish to clear the plate layout of all designations click the Clear all wells button A prompt will ask if you wish to proceed If you clear the plate layout all progress is lost and cannot be recovered 3 5 Viewing well information You may view a chart of your well information in the bottom section below the plate layout Figure 6 The Plate Layout chart includes information such as well assignment well ID dilution for unknowns and sample ID for unknowns To select the wells you wish to display in the chart first un select your mode at the top of the layout Then either drag your mouse across the grid you wish to see or double click the individual well you wish to see Cry Blaster Standards Unknown
10. J LLO L Serotonin Standard Curve Axis Standard f Pe US oo Linear X Axis Curves Linear x ais E C Display equation on chart C Display R 2 on chart Reports C Display ED50 on chart 90 100 110 120 130 140 150 160 170 180 190 200 21 Concentration ng mL www enzolifesciences com mag 22ers ALEXIS assay designs BIOMOL Stressgen Figure 12 Duplicate series No fit plot using Linear X Axis and Signal Signal Background Y axis Competitive LS issay Blasien OORA L Standard Curves Hsp27 Standard Curve Signal Signal Back Curve fit Sor nee X Axis PUR ERE DS Linear X Axis C Display equation on chart C Display R 2 on chart i Reports _ C Display ED50 on chart 400 500 600 Concentration pg mL www enzolifesciences com ag reo ALEXIS assay designs BIOMOL Stressger Figure 13 Duplicate series No fit plot using Linear X Axis and Signal Signal Background Y axis Immunometric 11 sray elasten EE wo Lel Standard Curves Plate Layout Hsp27 Standard Curve Curve fit Point to point v X Axis eS TAD Standard near xis E EDA Linear X Axis w C Display equation on chart Input Data C Display R42 on chart Reports C Display ED50 on chart 400 500 600 Concentration pg mL www enzolifesciences com ea ALEXIS assay designs BIOMOL Stressgeri Figure 14 Duplicate series Point to point plot
11. S J Enzo Life Sciences Assay Blaster User Manual Table of Contents TABLE OF CONTENTS seirce cecte cee ce eae cece ee ene ee ee oe ere ce een mene eset PERPE RESPE RETF E EFSER FEFE REFERS 1 We Getting Stante Usone aE Eaa 2 2 Selecting a New or Existing Experiment ccccccccsssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssssccs 2 3 Pte LOU a E 3 A UT A a E E E 8 Di Standa CO CS sasae E A E 10 6 RDO E EAE 15 fe RIFreportekamples siinse a a a 16 5777 Hines Drive Ann Arbor MI 48108 Tel 800 833 8651 or 734 668 6113 Fax 734 668 2793 www assaydesigns com orders assaydesigns com technical assaydesigns com Revision 06 30010 www enzolifesciences com f Getting Started 1 1 System Requirements Assay Blaster Data Analysis Software has been validated for use on PC computers running Windows 2000 or higher 512 MB RAM It has also been validated for use on Macintosh computers running 10 5 or higher 1 2 Install Assay Blaster Data Analysis Software Double click the Assay Blaster Data Analysis Software icon to start the installation wizard For PC computers click on the exe and for Mac computers click on the dmg Start the program by double clicking on the icon that appears Please contact Assay Designs Customer Support 800 833 8651 or 734 668 6113 if the Assay Blaster Data Analysis Software fails to install correctly Selecting a New or Existing
12. all copies thereof This License shall automatically terminate upon the failure of the Licensee to comply with any of the terms of this Agreement Sections 3 6 and 7 shall survive the termination of the License for any reason LIMITATION OF LIABILITY LICENSOR SHALL HAVE NO LIABILITY WITH RESPECT TO ITS OBLIGATIONS UNDER THIS AGREEMENT OR OTHERWISE FOR ANY INDIRECT INCIDENTAL SPECIAL OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY WHETHER FOR BREACH OF CONTRACT TORT INCLUDING NEGLIGENCE OR OTHERWISE ARISING OUT OF OR RELATED TO THIS AGREEMENT INCLUDING BUT NOT LIMITED TO LOSS OF ANTICIPATED PROFITS LOSS OR INTERRUPTION OF USE OF OR CORRUPTION OF ANY FILES DATA OR EQUIPMENT EVEN IF LICENSOR HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES THESE LIMITATIONS SHALL APPLY NOTWITHSTANDING ANY FAILURE OF ESSENTIAL PURPOSE OF ANY LIMITED REMEDY THE PARTIES AGREE THAT THE FOREGOING LIMITATIONS REPRESENT A REASONABLE ALLOCATION OF RISK UNDER THIS AGREEMENT DISCLAIMER OF WARRANTY LICENSOR AND ITS DISTRIBUTORS MAKE NO PROMISES REPRESENTATIONS OR WARRANTIES EITHER EXPRESS IMPLIED STATUTORY OR OTHERWISE WITH RESPECT TO THE SOFTWARE MANUALS AND DOCUMENTATION INCLUDING THEIR CONDITION THEIR CONFORMITY TO ANY REPRESENTATION OR DESCRIPTION OR THE EXISTENCE OF ANY LATENT OR PATENT DEFECTS AND LICENSOR AND ITS DISTRIBUTORS SPECIFICALLY DISCLAIM ALL IMPLIED WARRANTIES OF MERCHANTABILITY NONINFRINGEMENT AND FITNESS FOR A P
13. ating ALEXIS assay designs BIOMOL Stressgeni Figure 17 Duplicate series 5P Logistic Fit plot using Log X Axis and B BO Y axis Competitive To create a more precise curve fit you may delete outliers from your analysis by right clicking the desired point and clicking Remove button If you have chosen a single data series this will remove the entire 13 concentration point If you have chosen a duplicate or triplicate data series anew mean will be determined with the remaining points You may add these points back in by right clicking the point and selecting Add There are three options you may select between for the Y axis Signal Signal Background B BO and Signal All standard points are shown in a Linear scale X axis Signal or Signal Signal Background plot A zero standard will not be shown in a Log scale X axis or in a B BO plot You can switch between the linear or logarithmic presentation of the standard curve by clicking the Axis dropdown box and then selecting either Linear X axis or Log X axis The default fit is the no fit option You must select a fit before proceeding The application offers three types of interpolation models e Point to Point e 4P logistic fit e 5P logistic fit All curve models will show the selected unit of measure on the x axis against your selected Y axis If you choose Point to Point the application will interpolate between two adjacent points using a line y
14. e To select the wells you wish to display in the chart either drag your mouse across the grid you wish to see or double click the individual well you wish to see Bry Blaster Y Axis Label Mean OD 11 12 Plate Layout 6 7 8 9g 10 Input Data Standard Curves b 4 Yell ID i Sample ID Signal value 0 3 a 231 Unknown 8 1 Standarda o o www enzolifesciences com Enzo Life Sciences Qtrecegen incomorating ALEXIS assay designs BIOMOL Stressgen HOJ faj ee Figure 10 Well Information You may increase the viewing area by selecting the arrows on the border separating the chart and plate sections Proceed to the next page by selecting the Proceed to Standard Curves button or by selecting the Standard Curves tab 5 Standard Curves The Standard Curves page displays the standard curve created using selected parameters and settings for the standard data files Figure 11 displays the buttons and functions included on the Standard Curve page Proceed to Input Data 1 Notes Helo P Go to Enzo Life Sciences website 4 gt FA fssay Blaster Notes J 1 aag i lt li o L ejo a 8 IL 6 Standard Curve data Figure 11 Standard Curves page 10 Figures 12 through 17 show sample standard curve plots generated using various parameters LS issay Blaren LO
15. from any spreadsheet program You may not select a concentration of zero for more than one standard designation If you ran a background well select the standard by selecting the checkbox next to that standard value If your background is not a member of the standard curve check the desired Standard checkbox and leave the Edit Concentration field blank If you have checked a background check box but wish to deselect press the Reset Background button To continue analysis you must enter your analyte name as well as your desired unit of measure Ery Blaster s Standards Unknown Standard Replicate Unknown O D oe Analyte Name 2 v 12 IL 6 Edit Select Concentration Background STD 1 200 0 STD 2 h 00 0 STD 3 50 0 STD 4 25 0 STD5 12 5 STD 6 6 25 STD 3 13 STD 8 0 STD 9 STD 10 STD 11 B Laas Unit pg mL Reset Background www enzolifesciences com Enzo Live Sciences 3 A J vara ont Incorporating ALEXIS assay designs BIOMOL Stressgen Dio EE R E E Figure 3 Plate Layout Assigning Unknowns Samples Turn on the Unknowns function by highlighting the Unknowns button At this point you should select whether you want to populate your unknowns by column down arrow or row right arrow Switch your arrow selection by clicking
16. ifesciences com Enabling Discovery in Life Science North South America ENZO LIFE SCIENCES INTERNATIONAL INC LTD 5120 Butler Pike Plymouth Meeting PA 19462 1202 USA Tel 1 800 942 0430 610 941 0430 Fax 610 941 9252 customers info usa enzolifesciences com Switzerland amp Rest of Europe ENZO LIFE SCIENCES AG Industriestrasse 17 Postfach CH 4415 Lausen Switzerland Tel 41 0 61 926 89 89 Fax 41 0 61 926 89 79 Inf ch enzolifesciences com Germany ENZO LIFE SCIENCES GmbH Marie Curie Strasse 8 DE 79539 Lorrach Germany Tel 49 0 7621 5500 526 Toll Free 0800 664 9518 Fax 49 0 7621 5500 527 info de enzolifesciences com Benelux ENZO LIFE SCIENCES BVBA Melkerijweg 3 BE 2240 Zandhoven Belgium Tel 32 0 3 466 04 20 Fax 32 0 3 466 04 29 info be enzolifesciences com 2 Enzo UK amp Ireland ENZO LIFE SCIENCES UK Palatine House Matford Court Exeter EX2 8NL UK Tel 0845 601 1488 UK Tel 44 0 1392 825900 overseas Fax 44 0 1392 825910 info uk enzolifesciences com France ENZO LIFE SCIENCES c o Covalab s a s 13 Avenue Albert Einstein FR 69100 Villeurbanne France Tel 33 472 440 655 Fax 33 437 484 239 Info fr enzolifesciences com ALEXIS assay designs BIOMOL Stressgen 20
17. ifesciences com ag ror ALEXIS assay designs BIOMOL Stressgen Figure 16 Results page 15 7 RTF report examples There are 5 report components that may be included in your RTF 7 1 Input Data Tab Image Displays the raw data within the assigned plate layout 7 2 Notes Displays any notes made during your analysis 7 3 Sample Data Table Values Displays resulting concentrations for each unknown designation mi Hepes Analysis 1 Micnosotth rjr SEE Insel Format Tools Table window Help Type a question for help X Te i L ae ESS at al a FJ i00 i ie H Read H E z m le A Bor oe 8 Foros aye ae File File co b z aED x4 a E fr u i il 1 1 1 I na Assay Blaster Experiment Results Hsp Concentration Concentration Average Replicate 1 Replicate 2 Concentration Sample 1 4135 84 pgr mL 434 23 pg mL 424 04 pgr mL Sample 2 105 55 poy rnc 110 91 pg mL 108 23 poy rnc Sample 3 55 45 pov mb 128 43 pg mL Sec 1 Figure 17 Sample Data Table Results Page 4 16 7 4 Standard Curve Graph Displays the Standard Curve Graph as selected on the Standard Curves tabs Figure 17 Seles Type a question for help X Assay Blaster Standard Curve 5P Logistic fit curve fit Hsp27 Standard Curve Equations Y 107 364 0 10 107 36 1 1 1 0 x S401 1 0 07 4457 R 2 1 000 E050 3 401 41 Concentration pgm Fs E Page 5 Sec 1 5 6 At Ln REC TRE
18. isplay R squared on chart checkbox the R squared value will be displayed for that plot If you click on the Display ED50 on chart checkbox the ED50 value will be displayed for that plot See Figure 15 If selected these options will be displayed on the graphs in the RTF report Proceed to the next page by selecting the Proceed to Reports button or by selecting the Reports tab 14 6 Reports 6 1 Reports Page The reports page allows you to customize your RTF report Figure 16 RTF rich text format files may be viewed in most popular word processing software To select components of your report highlight the components and select the Include component in the report button To remove a selected component select the Exclude component from the report button To select multiple components hold down the control key while selecting You may rearrange the order of report components by selecting the component and using the Move up and Move down buttons Once you are Satisfied with your report components select the Generate RTF report button You will be prompted to save your report before viewing it If you would like to alter your report simply keep the Reports page open and re create your RTF report FA Assay Blaster BOR S Enzo Plate Layout input Data Tab Image Input Notes Data Sample Data Table Values Standard Curve Graph Standard Data Table Standard Curves www enzol
19. on the correct arrow Next select the number of unknown replicates in your analysis by using the Unknown Replicate drop down menu e g select 1 if only one well per unknown was run select 2 if duplicate unknown wells were run Select the wells you wish to populate as unknowns by clicking on a well and dragging your mouse to create the grid You may also double click on an individual well to either populate it or to un populate it The unknown designation e g Unknown 1 Unknown 2 is determined by the direction of auto population as well as your number of unknowns A minimum of one unknown must be selected Once you have populated your unknowns a chart listing your unknowns will appear in the left margin under the Unknowns tab Figure 4 You may alter the dilution factor for your sample as well as a Sample ID if you wish You may enter your dilution factor or Sample ID manually or by copying and pasting using the keyboard command Ctrl C and Ctrl V for PC or Cmd C and Cmd V for Mac If no Sample ID is provided your results will be listed under Y axis value of the first replicate ES Assay Blaster Unknown Standard Replicate Unknown Replicate 1 A Unknown 1 Wnknown2 Unknowns 1 o oOo o o m Input Unknowng h o ooo Data Unknowns h f Unknown Ao Unknown 1 o oOo o o Unknowns A oOo o o Standard Curves www enzolifesciences com ping ALEXIS assay designs BIOMOL Stressgen Figure 4 Plate Layout 3 3
20. pplications This software contains third party components with additional licenses that apply to these components A list of third party components can be found in the file THIRDPART YLICENSEREADME txt in the directory licenses in the application directory All referenced licenses can be found in that same directory This License will remain in effect until terminated in accordance with the terms and conditions specifically set out in the Agreement This License permits the Licensee to make one copy of the Software in machine readable form for backup purposes only Limitations Licensee agrees to treat the Software as confidential and not to copy reproduce sub license or otherwise disclose the Software or its associated manuals and documentation to third parties including any parent subsidiaries or affiliated entities without the prior written consent of Licensor Licensee agrees not to disassemble decompose reverse engineer or otherwise translate the Software Licensee agrees not to modify the Software not to allow access to the Software through any terminal located outside of Licensee s location and that any derivative works and the backup copy are the sole property of the Licensor Term This License is effective at the time the Licensee receives the Software and shall continue based upon the type of License indicated in the user documentation Termination shall occur when Licensee ceases all use of the Software and returns or destroys
21. tware is entered into in conjunction with the purchase of the license to the Software By installing and using the Software Licensee accepts the Software and agrees to be bound by the terms of this Agreement 1 2 7 Licensor s Rights Enzo Life Sciences Inc Licensor represents that it has all rights necessary to grant the license herein This software is protected by copyrights and includes valuable trade secrets and other proprietary material License Grant Licensor grants the purchaser Licensee a non exclusive non transferable license License under the such patent rights and or copyrights to use the Software at Licensee s designated site solely for its intended purposes for research and development in the life sciences field including the discovery of human and animal therapeutics and diagnostics the Research Field but not for diagnostics testing or for testing quality control and or the sale distribution or promotion of the Licensee s own research and diagnostics reagents This license enables the user to analyze and evaluate data from a variety of assays provided by Enzo Life Sciences In addition this software may be capable of analyzing data from similar assays offered by other manufacturers or developed as custom assays by end users When used to analyze data from assays provided by third parties or other parties the end user is responsible for establishing the compatibility of this software in these a
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