LCP 5020 User Manual
Contents
1. LCP5020 Section 5 DESCRIPTION OF THE PUMP Valve body Pipe Connecting piece Stop sphere Seat holder End sealing Sealing Seat Sphere o prom cms 0 3 0 5 6 Fig 8 Suction and discharge valve ther with the optical reader of the half revolution provides information on the pumping block cam position The information on the cam position is used for the control of the valve set during the creation of gradient by drawing up to three components of the mobile phase 5 2 4 Pressure relief valve The valve combines the function of pressure relief with pressure measurement The liquid pumped passes through the tensometric pressure gauge sensor The internal area of the sensor is filled with an insert which minimises the dead volume and ensures the offtake of bubbles The actual pressure relief is carried out by means of the screw with a closing cone controlled by a manual knob 5 2 5 Gradient valves The valve set for the creation of gradient on the low pressure side The set is an accessory which is mounted on the front panel It is connected by means of the intercon nection cable to the control units The presence of the valve set is tested by software During the realisation of the gradient the inlet of the pumped liquid is connected on a program basis to individual inlets of gradient components A B C at each suction stroke of the piston 5 3 Electronics2. The pump electronics is formed as a compact unit fed only by the voltage designed for the work of the motor namely within the range of 20 to 70 V DC 20 A in the case of LCP5020 it is 24 V DC 2 5 A All electronics besides the mains connection lead with a fuse and mains switch is installed on safe voltage The separation transformer features an approved electrical separation of 4 kV All voltages necessary for both analogue and digital functions are generated by means of an impulse source of this unit The unit consists of INGOS s r o Page 24 LCP5020 Section 5 DESCRIPTION OF THE PUMP two boards mutually interconnected by means of a connector and of a cooling board serving also for the attachment of the unit By means of connectors the complete set of the keyboard is connected to the unit so are the sensors and outlets 5 3 1 CPU board The board of CPU microprocessor contains Microprocessor INTEL series 51 PCF 80C552 32kB EPROM with the application software 32kB battery backup RAM 256B EEPROM for basic setting Communication RS485 with galvanic separation at request RS232 with the com munication protocol making it possible to connect as many as 64 units including PC to a local area network Differential analogue input for a tensometric pressure gauge 16 pin connector for the connection of the keyboard User s connector for 4 TTL outputs and 2 inputs Input from the in
3. 1 2 Waste 3 Input Fig 2 Pressure relief valve 1 Switch 4 Aux output 2 Fuse 5 Communication 3 Mains connection lead 6 Pressure output Fig 3 Rear panel Connection to the power supply and interconnection of other parts of the chroma tograph should be carried out only with the cables delivered by the manufacturer The connectors are not mutually interchangeable 3 1 4 Pumping block Inputs and outputs of the flushing liquid are interconnected with tubes on the sub panel In some cases it is possible to bring the flushing liquid directly to appropriate inputs and outputs of the pumping block see fig 7 connections on the subpanel being excluded Connection terminals of the tubes have again the cone LUER Note In the case of damage to the primary gland the pumped liquid will start to leak out through the flushing liquid inputs In the case of damage to the secondary gland the flushing liquid would leak through emergency apertures INGOS s r o Page 9 LCP5020 Section 3 PUTTING INTO OPERATION 3 2 Keyboard and display The two line display together with a 36 key membrane keyboard provides user s comfort for both direct control and program control Besides the control of the pump itself including gradient valves the program is capable of controlling such additional equipment as dispenser or laboratory robot possibly also a secondary pump during the creation of the h
4. PROG FLOW PH PL P MPa MD 1 88 48 B 8 7 lt coe a LCP 5020 M r V y y FLOW PRESS CYCLE LINE LINE C E d MOTOR 5 e lA BIC KC IN 2 IX 2 nM HELP INSERT DFELETE MODE MOTOR START RUN Fig 4 Keyboard PROG will switch the control system into program editing see k a INGOS s r o Page 10 LCP5020 Section 3 PUTTING INTO OPERATION from the menu MODE modification of the configuration of pump assembly operation see 8 1 0 entering of numerical data ENTER key for the confirmation of the number entered possibly for the selection gt direction keys for moving the cursor under individual display positions HELP will provide current user s information By double pressing you will call the in structions to use the HELP facility If you use the HELP function the keys ENTER and MN lt gt will be active The directional keys make it possible to make the selection by moving the cursor and the key ENTER will confirm selected options LIST will make it possible to look the list of programs and choose one of them for activation or editing If on
5. follows CYCLE Selected number of program cycles ACTU Number of the cycle currently run FINAL remaining number of the cycles not started The cursor will announce to you that the number of cycles can be changed The change will be carried out by typing the new number 4 pressing ENTER For example by entering the number 15 the display will change as follows INGOS s r o Page 14 LCP5020 Section 4 OPERATION CYCLE FIMAL 15 B 15 After the completion of the cycle 11 from the previous set the counting of the new file will start By entering the number 0 the set will be completed after the finishing of the cycle currently being run 4 3 Program editing Basic keys for program editing PROG turning on and off of the program mode If the program mode is on the LED at the prog key is lit INSERT LINE inserting of a program line before the line displayed DELETE LINE deletion of the program line displayed MN movement along individual program lines if the cursor is under the entry TIME At the entry into the program mode there will appear the first line of the program with the time 0 00 At the time 0 00 it is necessary to carry out the setting of all non zero parameters flow rate concentrations output control signals It is possible and at the time 0 00 it is often necessary to type several lin
6. gradient and flow rate values are computed The system also generates time commands for the co ordination with other apparatuses and peripheries of the liquid chromatograph set Level of monitoring and control of motor switching of gradient valves and pressure monitoring Level with the top priority is reserved for the communication with other apparatuses of the set and possibly with the master computer INGOS s r o Page 26 6 ACCESSORIES AND SPARE PARTS 6 1 Basic accessories 1 item Mains cord 3 items Tube for the connection of mobile phases 2 items Tube with the LUER cone for connection of the flushing system 5 items Passing screw UNF10 5 items Ferrule 1 item Stainless steel capillary tube 0 6 m 2 items Fuse T 0 5 A 6 2 Other accessories To the pump it is also possible to order the dispensing valve This valve is controlled manually it is fitted with response for the start up of the analysis see The valve has a replaceable dispensing loop Minimum volume of the loop is 20 pl To the valve it is also possible to order a holder for the attachment to the pump INGOS s r o Page 27 7 WARRANTY The pump LCP5020 is covered by the manufacturer s warranty for a period of one year from the day of handing over of the product to the purchaser The apparatus can be used only in the way provided for in these instructions The manufacturer will not bear responsibility for any damage caused b
7. only the information about the functions performed or about the values It is possible to modify the number of cycles see 2 4 After the start up of the program it is possible in exceptional cases to stop both the pumping and program run The execution of the commands by using the keys MOTOR STOP END and HOLD is conditioned by further confirmation of NO 0 YES 1 The confirmation can be carried out either by means of the cursor posi tion under NO or YES with the subsequent pressing of ENTER cursor is situated at the beginning under NO or by means of the keys 0 and 1 Thus the program run is protected from accidental pressing of the above mentioned keys The program run is interrupted by the key END Thus it is brought to the condition in which it is possible to carry out all interventions including the transfer to 4 3 Each pressing of the key END will return the parameters programmed to the values selected by the program line at the time 0 00 By using the key HOLD it is possible to stop the program run and by pressing it repeatedly it is possible to restart the continuation of the program During the time of the HOLD command activation the original time reading continues to run and all program controlled parameters and functions are fixed in the conditions in which they were at the moment of t
8. 1 Pumping block 4 union for the division of the input 2 Gradient valve into individual pumping heads 3 Subpanel of inlets 5 Pressure relief valve Fig 6 Area behind the front panel The central panel which carries the pumping block separates the open area from the closed area in the rear part of the apparatus This area contains a direct voltage source control unit and the pumping block drive 5 2 Functional groups of the pump 5 2 1 Pumping block This is a mechanism with a reciprocating drive of opposite pistons The floating arrangement of pistons makes it possible to carry out the flushing of pistons behind the primary high pressure gland and thus it is possible to pump also solutions with INGOS s r o Page 22 LCP5020 Section 5 DESCRIPTION OF THE PUMP crystallising substances The secondary gland furthermore protects the driving mechanism from aggressive liquid leakage during a damage to the primary gland The drive of the pistons is derived from the cam on which the pulleys positioned in the arms are turned The arms are firmly connected with the rods which are positioned in the block case on a sliding basis The springs supported by the rod rings press through the sliding blocks always on the arms of the opposite rod This arrangement is advantageous because the reciprocating movement of pistons is generated by the cam and the springs only balance the asymmetry of the cam At the same time also
9. ERATION Exit Flow Concentration Huxual outruts End of cucle Wait for switch Send mark Exit Program mod The line will be inserted in front of the line displayed The line inserted will offer the time of the previous line It is possible to modify the time within the range between the time of the previous and subsequent lines Software will not allow you to type the time out of the time sequence The basic types of the line can be inserted also by pressing the keys FLOW CONC AUX OUTPUT END without any further operation and the result will be the same as in the case of using the INSERT LINE Any number inserted needs to be confirmed by using the key ENTER The cursor will move to the following position and from the end of line to the beginning of the following line 4 3 1 Flow rate programming Flow rate selection depending on time The line is called by using the key FLOW PRESS 1 TIME FLOW 1 958 6 66 Cursor situated under TIME announces a possibility of jumps along the programmed lines in a time sequence At the same time it announces a possibility of rewriting the time of the line in question Time can be set within the range from 0 to 640 min with a precision to three significant digits This means 0 00 to 9 99 minutes by 0 01 min 10 0 to 99 9 minutes by 0 1 min 100 to 640 minutes by 1 min The flow rate between
10. INGOS Ltd INGOS HIGH PRESURE PUMP LCP 5020 User manual PRAGUE Apr 11 2006 Producer Development Supplier and service Laboratory Instruments division of INGOS Ltd PiKRON Ltd INGOS Ltd Tel 4 420 296 781 683 K Nouzovu 2090 420 296 781 692 14316 PRAGUE 4 Fax 420 244 403 051 CZECH REP e mail instruments ingos cz c INGOS 2006 INTRODUCTION CAPACITIES AND SPECIFICATIONS PUTTING INTO OPERATION OPERATION DESCRIPTION OF THE PUMP ACCESSORIES AND SPARE PARTS WARRANTY TABLE OF CONTENTS ORO GE Y LA 1 INTRODUCTION The pump is designed for analytical and semi preparative liquid chromatography It can also be used where it is necessary to ensure exact pumping of liquids of both low and high pressures and the liquids pumped can be strongly aggressive as well The new structure of the pumping block with floating pistons makes it possible to draw also solutions with crystallising substances The pump can be completed with a valve set which makes it possible to realise a three component gradient on the low pressure side The gradient on the high pressure side is realisable with two pumps For semi preparative chromatography where the flow rate 20 ml min is insufficient it is possible to order the pump LCP5080 with a flow rate up to 80 ml min The two line display together with the 36 key membrane k
11. If the numbers do not corre spond check carefully all packing material If any item is missing please contact the producer or your supplier Put the pump on the table and make yourself familiar with the arrangement and functions of control and connection items Before the first activation of the pump read thoroughly the instructions concerning its operation see The equipment does not have any requirements on additional ventilation 3 1 1 What you need In order to put the pump into operation you will need the following items 1 Other parts of the liquid chromatograph dispensing valve column detector One or more bottles with a mobile phase We recommend to use the bottles whose plugs are fitted with passages for tubes It is suitable to use the tubes equipped with input filters The actual tubes must be resistant to pumped liquids These bottles must not be put on the pump 3 Appropriate pipes and connection material serving for the connection of the dispensing valve and all parts of the chromatograph 4 In the case of realisation of the gradient on the low pressure side it is possible to ensure a perfect function only in the case of degassing of the mobile phases with helium or by a special degassing device 5 In the case of pumping the liquids containing crystallising substances it is necessary to have a flushing liquid in most cases distilled water The flushing liquid should impede crystallisation and dissolve possible crystal
12. R By moving the cursor with the keys lt gt under the or PL positions it is possible to set the pressure limits within the range from 0 to 40MPa The upper pressure limit PH will stop pumping always when the pressure is exceeded in all modes of the pump operation The lower pressure limit PL signals or stops the pumping in all modes except for the pumping in the PURGE mode In the mode MODE see 5 it is possible to specify whether to stop pumping if the pressure drops under the PL limit or whether the pressure drop should be signalled only The last position of the display shows current pressure P MPa The pressure is displayed on the display at each stroke of the piston If the pumping is stopped the pressure is displayed once in 0 01 min Note After the activation of the pumping by using the START command the software checks for correct start up of the pressure above the lower pressure limit If the pump is properly vented and the pressure continues to rise after the start up of the pump no failure message is displayed even if the pressure remains below the PL level If the pressure continues to drop at the start up there is displayed an error message In this case it is suitable to reduce the PL limit to zero and find the reason 4 1 2 Low pressure gradient The isocratic pumping of up to three components at the pump fitted with the valve set for the realisation of a gradient on the lo
13. S 2 1 Capacities Precise efficient and reliable mechanics together with modern electronics and soft ware provides a universal use while maintaining a standard price 2 1 1 Pumping block The pumping block with the floating positioning of pistons and with a possibility of piston flushing behind the primary gland is suitable for the pumping of all liquids used including solutions which crystallise in air The range of selectable and fully reproducible pumped flow rate from 0 05 to 20 0 ml min at pressure from 0 to 40 MPa mean that the pump is suitable in both analytical and preparative HPLC 2 1 2 Gradient After the filling of the pump with a valve set it is possible to realise a three component gradient on the low pressure side The software makes it possible to provide isocratic pumping as well as the program selection program can be entered by means of the pump keyboard or by a master computer if there is any in the assembly The gradient with almost unlimited program selection supports all work in the analytical HPLC at recommended flow rates from 0 2 to 5 ml min This flow rate limitation results from the actual principle of the creation of a low pressure gradient In the case of larger flow rates it will not be possible to ensure precision and linearity For all flow rates it is possible to realise the gradient creation on the high pressure side by connecting two pumps For this use the pumps are fitted with serial communicatio
14. a perfect linearity of the piston drive necessary for the lifetime of the glands is ensured The arms influence the pistons fitted with a spherical area only in an axial direction The pistons are conducted only through the primary and secondary glands The pistons positioned this way are referred to as floating The area between the glands through which the piston passes can be flushed with a liquid capable of dissolving possible crystals The inlets fitted with the cones LUER serve for the delivery of flushing liquid Eus 1 inspection windows here it is possible 4 Heads to see piston movement 5 Piston flushing system offtake 2 Piston flushing system inlets 6 Entry valves 3 Exit valves Fig 7 Pumping block 5 2 2 Valves Suction and discharge valves are installed in pipe unions see fig 8 The valve functions is carried out by a sphere and cone seat All valves are of the same construction and they differ only in the order of assembly of the valve seat and sphere to the valve pipe union It generally applies that the seat is situated under the sphere For a perfect function the valves are doubled in each pipe union 5 2 3 Pumping block drive The pumping block is driven with a direct current motor with permanent magnets Motor revolutions are reduced by means of a double transmission with dented belts which can transfer the torque from the motor to the pumping block The optical encoder toge INGOS s r o Page 23
15. ated under the pumping block This check should be carried out after each start up after 30 minutes of the operation if possible Any possible leakage should be removed by tightening the loose connection Remove spilled liquid by using a suitable cloth Using the apparatus in a manner other than the one specified in the instructions can endanger general safety 4 8 Troubleshooting Most of incorrect requirements on the pump are shown on the display Monitor the operation liquid levels in order to protect the pump from aeration In case of other defects call service technicians or the manufacturer INGOS s r o Page 21 5 DESCRIPTION OF THE PUMP The pump is a compact apparatus which associates demanding mechanics with mo dern control electronics fitted with an in built microcomputer Perfect servicing is made possible by easy access and replaceable nature of all functional groups and subgroups High rigidity of the case structure makes it possible to place more apparatuses of this construction on one another 5 1 Pump case The front part of the apparatus is formed by a plastic moulding fitted with a mem brane keyboard Behind the front panel you will find an open area in which all hydraulic components with all necessary interconnection are situated see fig 6 The bottom part of this area is formed by the stainless steel tray which can collect a possible escape of liquid during the debugging of the apparatus
16. cremental sensor of position with a 24bit reader for the current position of the motor with the cam dead centre control Other I O signals signals of impulse width modulation and signals for A D converter are interconnected with a connector to the board of the feeding source and switches 5 3 2 Switch source board The board of the source and switches includes Impulse source for input voltage from 20 to 70 V DC which generates all necessary voltages Full galvanic separation from signals as well as from the feeding of digital circuits Switch of the output for the motor up to 20 A 70 V DC controlled by impulse width modulation within the range of 0 to 10096 with a frequency of 20 kHz Electronic fuse against overload and overheating This fuse is set at LCP5020 to 3 A Switch of up to four gradient valves with the time of switching on and off of the valve shorter than 2 ms 5 3 3 Keyboard The keyboard complete assembly associates the actual membrane keyboard board with LCD display for 2 x 16 characters and LED diodes The board is connected with CPU by means of a flat cable with connectors 5 3 4 Rear panel The rear panel of the pump is fitted with connectors accessible to the user which are connected to it by means of interconnection cables see fig 3 Mains connection lead with the fuse T0 5 A and a mains switch The fuse is installed in the holder which can switch over the voltage through its position This function
17. dispensing valve 20 47 Routine maintenance and servicing o rr LC ERE RR dares ETA Troubleshooting suscritos ici Bal DESCRIPTION OF THE PUMP ipiis 2 0 P mp SnecexerLever SEX cov dhe T EE ces pases ob toes C CGU REI AUGE ae ss BJ 5 4 Functional groups of the pump vaste XA REO bros BJ INGOS s r o Page 29 LCP5020 Section 8 TABLE OF CONTENTS LO 521 Pumping block P BJ Valves ae meee detec E E E AE E E Pumping block drive eren ERES ot evel BJ 524 Pressure relief valve 24 Gradient valves uocatis De GU iaa DA Electronics A A M EE ede qu pe Eid xac 24 AAA ONT T ITI t EDIT Switch source board ene bed otal coi Fe COE ECRIRE 53 3 Keyboard 5 3 4 Rear panel 54 Software A 26 6 ACCESSORIES AND SPARE PARTS Rd ded ee SIE doro EE Basie Ste seme see icy Other a L WARRANTY AS TEE GUENE EE EUSE DEIEK EE Aii na ENa DE L I Waste disposal sisas ii is 28 El TABLE OF CONTENTS 43509 2 085 24 437A UICE REI d dak ROAD dp xL oae tad d 29 List of picturtes and tables ua ioa ie EE Teri ride t BO 8 1 List of picturtes and tables bug L Subpanelb o ub Ped asque ra doe Pb uat dep did 8 Fig 2 Pressure r
18. e824sav PTT 2 TTL level OMNES cutis ridad bases 4 TTL level Gradient High pressure cdta ebbe eed with two pumps Low pressure viii sarral as her three component with additional valves PICCISION do a piano Yen es 1 Dimensions w x h x d erc 240 x 130 x 360 mm Weight serere tu dd vean nei Re ted 12 kg Power supply iia accepte 230 V 10 50 Hz Category of overvoltage in the installation II Per Inputs sida sarita 60 VA Depends on flow rate and pressure The pump LCP5020 is designed for the pumping of liquids used for the high pressure liquid chromatography which does not spoil the pump materials which are in contact with the pumped liquid These are stainless steel 17246 PEEK TEFLON and sapphire At a special order it is possible to replace steel with PEEK The apparatus is designed for the environment featuring a temperature from 15 to 35 C humidity up to 80 without acid and caustic vapours The operators must be instructed with regard to safety regulations and work with the liquids used INGOS s r o Page 6 3 PUTTING INTO OPERATION 3 1 Unpacking Unpack carefully the pump from the transportation package Check the external appearance of the apparatus and verify whether the apparatus has not suffered any visible damage during transportation If the apparatus was damaged during transport contact immediately the transport company According to the list of accessories 6 1 check all items
19. elief valve ic A 0 Fig Hear panel Fig 4 Keyboard TO Fig 5 Development of concentrations for the presentation program 107 Fig 6 Area behind the front panel RR Ra ACA BJ Fig Pumping Plock rte ds basis cado r eaa a a AE 23 Fig 8 Suction and discharge valve uc ii di 24 INGOS s r o Page 30
20. es with the same time To the same time it is possible to assign also two different values of the same pa rameter The change in the parameter will be realised by means of a jump in the line sequence During the first typing of the program or during the extending of the program the last line is marked as End of program This represents only the identification of the border between the program and free memory If no line for the program completion has been typed the programmed parameters will keep the values given by the last program lines for the parameter in question The program which is not completed by the line END will run for up to 640 minutes unless it is interrupted manually by using the key END The program terminated by the line END and by the typing of the time will release after its completion to the given time an audible signal and will write End of program All programmed parameters are provided for with the values corresponding to the time 0 00 If the user does not use the cycling of the program see 4 2 4 it is necessary to confirm the completion of the program by pressing any key The key INSERT LINE makes it possible to insert a program line into the written program After pressing INSERT the display will show the menu from which you will choose the type of line with the help of and ENTER INGOS s r o Page 15 LCP5020 Section 4 OP
21. eyboard provides user s comfort for both direct control and for program control Before controlling the actual pump including gradient valves the program can also control such additional devices as dispenser or laboratory robot possibly also the secondary pump during the formation of a high pressure gradient The pump is constructed as an independently working apparatus and it can be connected to the centrally controlled purpose set consisting of more apparatuses Serial communication makes it possible to connect more apparatuses into a complete assembly for HPLC or for other purposes It also makes it possible to control the entire complete set and to assess the measurement by a master computer Besides the serial communication the apparatus is fitted with a connector with TTL signals for the control of peripheries which do not have any processor built inside Caution In terms of its power output the pump LCP5020 is exceptional and fully different from common pumps used for the analytical HPLC Before the turning on of pumping by means of the pushbutton MOTOR START check the flow rate on the display If the flow rate is excessive the column could be damaged The flow rate of 8 ml min activated with the key PURGE will be stopped if pressure of 1 MPa is exceeded The connection material is fitted with a standard thread for HPCL UNF10 32z Be sure to use the screws M5 INGOS s r o Page 4 2 CAPACITIES AND SPECIFICATION
22. he activation of the command HOLD The HOLD function is indicated by the lighting of the LED situated on the HOLD key and by the flashing value of the current time After the repeated start up of the program run to be carried out INGOS s r o Page 13 LCP5020 Section 4 OPERATION by another pressing of the key HOLD the display shows the time returned to the value from the moment of the beginning of HOLD 4 2 1 Display of current time flow rate and pressure By using the key FLOW PRESS you will call the following display during the program run TIME FLOW P MPa 1 34 2 35 12 5 On the display you can see current values of time flow rate and pressure 4 2 2 Display of time and concentrations Display of time and current concentrations of the low pressure gradient formed by the valve assembly will be called by using the key CONC TIME lt A 46 AL 1 6 48 23 29 Display of time and concentrations of the high pressure gradient formed by two pumps TIME M ao 5 48 3B TH 4 2 3 Display of inputs and outputs The key AUX OUTPUT will call the display of the current state of output com mands of the TTL level and responses TIME e 1 37 1234 56 4 2 4 Display of the number of cycles Display of the pre selection of program cycles key CYCLE CYCLE ACTU 2H 11 3 The meaning of individual items is as
23. igh pressure gradient Functions of individual keys are as follows MOTOR START will activate the pumping at the selected flow rate see 1 1 Pumping is indicated with LED and also the upper and lower pressure limits are checked PURGE switches between the pumping at a selected flow rate and the flow rate PURGE This is set to 8 ml min which is a value suitable during the work with an analytical column In the case of higher flow rates this function is not necessary In the purge mode the lower pressure limit is not checked The upper pressure limit is set to 1 MPa The operation is indicated with a LED Return will be carried out by the repeated pressing of PURGE or MOTOR START MOTOR STOP stops pumping It is necessary to confirm the stopping also in the program mode see 2 A B C at the pump with valves for the gradient on the low pressure side will switch over the mobile phase components The active component is indicated by a LED After turning on the component will be active RUN will activate the program control of the cycle if the program is in the internal memory HOLD will suspend the program run END will interrupt the program and return all time dependent values to the con dition as they are entered by the program at the time 0 00 see 3 also in the case of direct control see 4 1 LIQUID CHROMATOGRAPHY PUMP
24. is unused and the holder should be in a position of 220 or 240 V INGOS s r o Page 25 LCP5020 Section 5 DESCRIPTION OF THE PUMP AUX OUTPUT Connector for the control of auxiliary devices 4 TTL outputs and 2 inputs for back reading COMMUNICATION A couple of connectors of the communication RS485 RS232 Connector for the connection of the line recorder for pressure registration 5 4 Software Software of the pump LCP5020 controls all functions mediates contacts with user through the keyboard and display communicates through RS485 with other apparatuses of the purpose set including a computer With regard to the performance of a large number of time demanding operations the software is arranged in the following levels At the basic level there are processed commands of the user who has a permanent access through the keyboard to the information concerning the operation of the pump and to important commands In all modes it is possible to enter the STOP command end of pumping and the HOLD command program run interruption If the display cursor is situated on a position where is also the help facility it is possible to call this help by using the HELP command Level of realisation of commands from the master computer The commands adopted at the highest level are sent to the input queue are decoded and sequentially realised Level of execution of the time program At this level interpolations for the
25. kes it possible to program and store more programs entry into the multi program mode will be carried out by using the key LIST HUM PROGRAM 1 Benzen The programs are automatically numbered and it is also possible to assign a name to the program number If the first program was programmed without using the functions designed for more programs it is stored under the number 1 and the symbol announces that the program exists The name can be created by moving the cursor gt to a particular position and by using the keys N W it is possible to choose letters numbers as well as special characters The program selected should be confirmed by ENTER Thus you will leave the selection mode and it will be possible to start the program The term activisation stands for your pressing the key END Should you fail to do so the help function on the display will provide assistance to you It is possible to edit the program selected see 3 If you are programming a new program it is sufficient to confirm in the mode LIST the last number of the program and to carry out programming in the mode 3 The name can be added on an additional basis In the mode LIST it is possible to delete the program by using the key DELETE LINE After the deletion of the program the programs having higher numbers will be renumbe red 4 5 Setting of f
26. ly one program is pre programmed it is not necessary to use this function see 4 4 CYCLE AUX OUTPUT CONC FLOW PRESS these keys serve for the swit chover of individual displays IMPL INSERT LINE DELETE LINE of the program see serves for the switchover of individual components of the gradient see these keys are used during the creation INGOS s r o Page 11 4 OPERATION 4 1 Direct control Functions of the keyboard and display at the time when no program is running It is possible to control the pump regardless of the program stored in the internal memory After a possible completion of the program run or interruption of the run by the key END it is possible to control the pump immediately by using this way All time dependent values are brought to the configuration after the END command when they were entered into the program for the time 0 00 see 4 3 The same will happen also after the starting of the apparatus 4 1 1 Selection of flow rate and pressure limits If you press the key FLOW PRESS you will call the following display arrangement FLOW PH FL P MPa 2200 280 5 12 8 The cursor announces to you the possibility of a change in the flow rate It will be carried out by typing a number within the range from 0 00 to 20 0 ml min and by pressing the key ENTE
27. n The program can be entered by means of the keyboard of a pump or by the master computer 2 1 3 Memory All parameters pre set including the program are permanently stored in the memory also after the turning off of the apparatus power supply 2 1 4 Inputs and outputs For the purpose of the controlling of additional devices which do not have their own intelligent systems the pump is fitted with input and output facilities TTL level signals 2 2 Specifications Flow Tate TER 0 05 to 20 ml min Flow rate reproducibility 0 1 Piston stroke volume 0 055 ml Maximum pressure 40 MPa Pressure limits 1424220 Descr re ett Hen 0 a 40MPa optional by 1 MPa Pressure measurement by tensometric sensor presentation digital on the display INGOS s r o Page 5 2 LCP5020 Section 2 CAPACITIES AND SPECIFICATIONS Output for recorder 3 cete e cete 10 mV 50 MPa Control Keyboard iin cancel oe deat PEE IHRER teas membrane 36 keys display 2 x 16 characters Time program ha tha 500 program lines in the time to 640 minutes can be divided into more independent programs Controlled parameters flow rate gradient concentration output control signals number of cycles Communication 22 ed RS485 or RS232 Control signals IGS s 524554 q
28. r the next time interval The repeated pressing of the number will both activate and cancel the command 4 3 5 Input control signals With the help of the key INSERT LINE insert by selecting the item Wait for switch into the program a command for waiting until the input signal comes TIME 4 66 Wait for SW The program will stop on this line The time continues to run but is flashing The program is interrupted in the same manner as if you press the key HOLD up to the moment when there arrives a signal from the input 5 Then the program run continues and the time displayed will return to the time of the line Wait for switch and from there it continues to run 4 3 6 Sending a mark With the help of the key INSERT LINE by selecting an entry Send mark you will enter into the program a command designed for the sending of a mark through the network TIME Mark to ABR B du 2 This command will at the appropriate time send MARK through the network to the apparatus given by the address ADR 4 3 7 Program termination By using the key END you may enter the line for the end of program The system will terminate the program run and set the parameters entered at the time 0 00 TIME 8 78 INGOS s r o Page 18 LCP5020 Section 4 OPERATION 4 4 Multi program mode The operating memory of the in built computer ma
29. ront panel The actual pressure relief is carried out by rotating the control knob activating a larger flow rate 8 ml min usually initiated by the command PURGE The pressure relief can be terminated by lowering the flow rate and closing the valve It is necessary to observe the order of operations 3 1 3 Rear panel The rear panel is fitted with all electrical connection elements necessary for the con nection to the mains control pressure measurement and all communications see 3 Aux output Connector with logical signals of the TTL level for the control of additi onal equipment 4 output control 2 input for confirmation of the function performed Through the mediation of this connector it is possible to control also relatively compli cated systems e g for the preparation and dispensing of a sample and the equipment in question need not be fitted with its own intelligent system Communication A couple of the network connectors ALAN for a two wire intercon nection of the entire set of instruments associated in a purpose assembly The network uLAN is electrically compatible with the RS 485 standard Alternatively it is possible to deliver RS232 Pressure output Connector with an analogue signal for pressure measurement 10 mV 50 MPa It makes it possible to connect a line recorder during the pressure registration INGOS s r o Page 8 LCP5020 Section 3 PUTTING INTO OPERATION 1 Output
30. s The flushing can be ensured on an occasional or permanent basis according to the properties of the substance pumped Practical note concerning point 4 In most cases it is possible to operate a low pressure gradient without degassing equipment By using a suitable method it is possible to rea lise a gradient from 100 water to 100 methanol The mixing of clean components is practically impossible without degassing equipment It is however possible to go through the phase of 50 water 50 methanol This phase is mixable without any problem with both water and methanol 3 1 2 Control and connection elements The mains switch is situated on the rear panel on the right After its activation the display background will be lit together with usually one gradient component The front panel is fitted with a membrane keyboard and a display of 2 x 16 characters INGOS s r o Page 7 LCP5020 Section 3 PUTTING INTO OPERATION l Piston flushing system outlet 4 Input of component B 2 Piston flushing system inlet 5 Input of component C 3 Input of component A Fig 1 Subpanel of inlets The subpanel of gradient component inlets inlet and waste systems of flushing liquid serving for the flushing of pistons between the primary and secondary glands is situated on the left behind the front panel see 1 The pressure relief valve see with outlet and waste threads is situated on the right behind the f
31. t Weed 7 all Whatyou need AP big big eer bebat m Control and connection elements esses a E ALA panel B Pe deis es D 5 4 Keyboard and display estais edad Ua d TO EI OPERATION A e ples RP AERE nS T2 LAT Direct control ii ep RRA eg een SEAE ge T2 4IT Selection of flow rate and pressure limits I2 Low pressure gradient do Iu gud boi vane ees I2 41 3 Control inputs and outputs Rr pU RR 13 4A Programi t n at 13 424 Display of current time flow rate and pressure L4 L 427 Display of time and concentrations exter de I4 ADS Display of inputs and outputs iii e REFER HOA is 14 E 724 Display of the number of cycles een AD Program is A ARA eR ETE EN A L5 43 Plow rate programming pex L6 Lu Low pressure gradient L6 Ava High pressure gradient E gae d 434 Output control signals IS 43 5 Input control signals id ES Sending a mate AnD EEE ES Program termination eii is ES _ 1 Multi program mode dra ERE tees 9 44 Setting of further parameters Vip kes 19 4 6 Start of analysis by using the
32. the values of individual lines will change on a linear basis If you want to use a jump change of the flow rate it is necessary to enter two lines within the same time 4 3 2 Low pressure gradient Selection of concentrations realised by three valves situated on the low pressure side precondition is represented by the valve set installed The line is called by using the key CONC TIME AB al 2 58 3B zh INGOS s r o Page 16 LCP5020 Section 4 OPERATION It is possible to select concentrations for the components B and C the component A will be completed to 10096 In the time interval between individual lines the concentrations are computed in such a way that the passage from one concentration entered to another one is linear If the line in question is to be used for the control of only one component and the second component is controlled by other lines instead of the concentration of the second component it is necessary to enter the symbol by using the key IMPLIC In fig 5 you can see the development of concentrations for this program TIME AB acl B B 1 68 24 2 zh 2 18 Eie 1 Concentration of the component A n 2 Concentration of the component B Bod a LO 3 Concentration of the component C 0 1 2 3 4 TIME min Fig 5 Development of concentrations for the presentation program 4 3 3 High press
33. ure gradient Creation of a high pressure gradient by controlling the flow rate of two pumps A precondition is given by two pumps LCP5020 mutually interconnected both hyd raulically and electronically by using the mains connection cable LAN and by correspon ding setting of parameters in the mode MODE Nothing is entered on the controlled pump SLAVE On the controlling pump MASTER it is necessary to select the development of all parameters controlled including of the MASTER pump flow rate The percentage of the SLAVE pump flow rate is additionally calculated TIME M A5 5 48 3B TH If the pump MASTER is at the same time fitted with a valve set it is possible to switch by means of the program between individual components A B C The switching will be programmed by inserting the item SOLUTION from the menu INSERT LINE INGOS s r o Page 17 LCP5020 Section 4 OPERATION TIME SOLUTION 3 1H H 4 3 4 Output control signals The key AUX OUTPUT will call a modification of the display making it possible to control by means of the program the output commands signals S If the cursor is situated in the field TIME it is possible to change the time of the programmed line TIME 5 AUR 1 37 1234 By using the keys 1 2 3 4 it is possible to change the condition of the output commands fo
34. urther parameters The following instructions serve for the entering of the functions being out of the possibilities of a direct selection from the keyboard modification of software for the work in an assembly of apparatuses and for the obtaining of the information which is not available by means of basic commands of the keyboard Entrance by pressing MODE Exit by pressing FLOW CONC AUX CYCLE It is also possible to escape by pressing RUN and PROG while the functions of these keys are being performed After the entrance into the mode MODE the display offers the following menu INGOS s r o Page 19 LCP5020 Section 4 OPERATION Exit test Monitor Processor sPeed Memory clear Communication Grad by flow Gradient valves User Limer alarm Individual entries have the following meanings ELLO asp rU C evens ced Exit from MODE Hw test adici n Test hardware For servicing purposes only Monitor Access to registers and processor memory For servicing pur poses only Processor speed Processor load test For servicing purposes only Memory clear Program deletion and deletion of all values stored Communication Selection of forms and parameters of communication Grad by flow ciii Selection of the manner of creation of the gradient with the help of two pumps Gradient val
35. ves Selection of the manner of the gradient creation with the help valves User time alarm Time counting The selection is carried out by using the cursor keys and ENTER 4 6 Start of analysis by using the dispensing valve If you have a dispensing valve with response you can program your program in such way that the analysis can be started by turning the valve Into the program it is necessary to insert at the time 0 00 theline Wait for switch see 4 3 5 TIME Wait for SM B du This line will ensure that the program will wait until the dispensing valve is turned As the following line at the time 0 00 it is necessary to insert Send mark to address TIME Mark to ABR B du 2 This line will ensure the sending of the mark to address 2 Address 2 is assigned to the computer and this mark will start the integration program INGOS s r o Page 20 LCP5020 Section 4 OPERATION 4 7 Routine maintenance and servicing The pump LCP5020does not require any special maintenance besides the flushing of the area between the high pressure and low pressure glands during the pumping of the crystallising substances puffers After the connection of the output pipes it is necessary to carry out a visual inspection for tightness of metal connections Any possible leakage will be demonstrated by the drops of liquid on the bottom of the stainless steel tray situ
36. w pressure side After the connection of the connector to the valve chamber the software will recognise the installation of the set into the apparatus Thus it is possible to confirm automatically the modification of the pump for the creation of the gradient on the low pressure side INGOS s r o Page 12 LCP5020 Section 4 OPERATION Display for the setting of concentrations is to be activated by using the key CONC FLOW lt A XB XC 2 28 56 38 It makes it possible to enter the concentration of the component B alternatively the concentration of the component C within the framework from 0 to 10096 The component is computed additionally At the same time it is also possible to select a flow rate Let us note that 100 of each component can be called by using the keys A B C and this also applies to any other display configuration 4 1 3 Control inputs and outputs Control of output commands and reading of responses ALI mm ES OUTPUT 1234 56 Input commands TTL signals S are controlled by means of the numerical keyboard where the pressing of the keys 1 to 4 leads to the activation of the command and repeated pressing leads to its cancellation The responses R are displayed only 4 2 Program run After the start up by using the key RUN the LED of the key RUN lights it is possible to call
37. y failure to comply with the conditions provided for in these instructions The pump LCP5020 is designed for the pumping of the liquids used for high pressure liquid chromatography which do not spoil the materials of the pump being in contact with the liquid pumped These are stainless steel 17246 PEEK TEFLON and sapphire The apparatus is designed for environments featuring a temperature of 15 to 35 C humidity up to 8096 without acid and caustic vapours The operators must be instructed with regard to safety regulations and work with the liquids used All warranty and after warranty repairs shall be carried out by the manufacturer or by an organisation authorised by the manufacturer 7 1 Waste disposal When the instruments operating life is over dispose it in respect to valid regulations also it can be returned to the vendor or producer for liquidation Warning Instrument contains parts PCB s which are rated as hazardous waste INGOS s r o Page 28 8 TABLE OF CONTENTS Li INTRODUCTION a D CAPACITIES AND SPECIFICATIONS Capacities T RS block ede sd cade eU aa pins and 2 4 Specifications AAA y Ea A EEEE REER BJ PUTTING INTO OPERATION wed a otras 1 31 Unpacking bli obo Det boe bbb do
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