FB12 Single Tube Luminometer
Contents
1. 7 system description ss 3 technical data seen 43 tool o Djsto 1a PRIMER RENT tt M EN 16 transport instructions 45 typographical conventions 2 47 FB12 Luminometer 16 Decontamination Form 16 Decontamination Form Any laboratory instrument used for clinical or research analysis maybe considered a biohazard and requires decontamination prior to handling Universal precautions are suggested wherever applicable Before shipping any instrument back to Berthold Detection Systems the luminometers have to be cleaned in the following way 1 Wash the housing with a moist cloth if necessary use a mild detergent or disinfectant Do not use any scouring agents 2 Open the access door remove and clean the reflector then clean the measurement chamber with the measurement window carefully Use e g cotton buds for hard to access locations Berthold Detection Systems only accepts instruments for repair together with a filled out decontamination form Please copy this page and fax it to us before you send off the package Thank you Instrument Type Serial Number confirm that the specified instrument was decontaminated according to the above described decontamination procedure confirm that the above specified instrument had no contact to any hazardous material Company Contact Person Position Tele2. MEAS ABORTED REPEAT DISCARD REPEAT Repeat measurement of replicate sample DISCARD Measure next sample The measured values are calculated depending on this selection Data output on display Only raw data in RLU s are displayed Berthold 8 DETECTION SYSTEMS FB12 Luminometer 3 Measurement in Stand alone Mode 3 2 Raw Data Measurement In the raw data mode samples are measured and numbered consecutively Creating a protocol FB12 LUMINOMETER MEASURE MEAS SET NEXT DELAY TIME 4 0 amp SCROLL CURSOR NEXT MEASURING TIME 10 0 s SCROLL CURSOR MAIN Q Define delay time and measurement time Q Press MAIN to return to the main menu Raw Data Measurement Q The display shows the main menu FB12 LUMINOMETER MEASURE MEAS SET READY 30 RLU S D 2 08 T 10 08 MAIN Q Insert sample tube and close sample drawer The measurement starts MEASURE 4500 RLU s D 2 0s T 10 08 MAIN Q At the end of the measurement time the following display appears DONE 4500 RLU s REMOVE SAMPLE MAIN n If the interface has been set to PRINTER you can here choose between raw data and cut off see chapter 11 Berthold DETECTION SYSTEMS 3 Measurement in Stand alone Mode FB12 Luminometer 3 3 Check Mode The check function enables the user to check the photomultiplier if no measurement is running The measured RLU values are displayed continuously regardless of whether the sample drawer is open or close
3. Luminometer 4 Working with the FB12 PC Software 4 3 Basic Software Structure Protocol Manager 4 FB12 Sirius Protocol Manager G x m Protocol types Figure 4 Em 1m Structure of c Single Kinetics FB12 PC program Dual Assay computes the sform of net values from each sample which has two series of Options replicates z Opening a data m Runnable protocols fi le Run Quick Measurementis a fast and simple protocol to take measurements with the FB12 pelete Create a protocol Save Measurement menu ics Datal Single Kinetics Parameters Single Kinetics Test Total duration Sampling time aa 4 Fre foes see pe 4 DLAMEL a Note Interval time Total duration No of data points to nearest 0 2s p Start timer by Start button or space bar or enter key No of replicates Door close B a Comment Advanced Options Excel TM Options J EXCEL transfer options Kinetics Graph Single Kinetics Excel TM Options Single Kinetics Test Tv Auto send to Excel TM at measurement completior Workbook JCAProgramme FB12 Software Wor Browse ance Worksheet Lumitest At cell p z F these feature Eat SSC
4. Softkey Function key enter Meaning and operation of individual functions Measurement in stand alone mode after input of measurement parameters and configuration Define the measurement parameters for stand alone operation If the interface is set to PRINTER you may choose parameter entry either for a raw data or for a cut off measurement see chapter 11 Switchover to PC OPERATION This function is not available if the interface has been set to PRINTER CONFIG Define the system parameters CHECK Here a continuous measurement is performed see chapter 3 3 Confirm each entry or new setting with lt ENTER gt If you want to keep a setting you may proceed directly with NEXT or MAIN CURSOR Move the cursor left SCROLL The highlighted digit or letter is incremented by 1 cyclical order 0 1 9 0 1 and A B Z A Berthold DETECTION SYSTEMS Menu Structure of the Instrument Software CUT OFF MEASUREMENT Serial port set to printer and Cut off protocol selected Serial port set to printer INSERT NEGREPL1 READY SD BEDS PROTOCOL RD CUT OFF CLOSE DOOR MAIN D 2 0s T 10 0s MAIN SCROLL MAIN NEXT PC OPERATION 30 RLU s DOOR OPEN END SYSTEM CONFIG CHECK NEXT 8 erial port set to PC CUT OFF MEASURE 30 RLU s NEGREPL 1 D 2 0s T 10 0s D 2 0s T 10 0s MAIN PC OPERATION 30 RLU s OPERATOR ccsssesssseseseesese DOOR CLOSED END SE RS232 FOR PC PRINTER SCROLL CURSO
5. Window gt Tile Vertical Berthold DETECTION SYSTEMS FB12 Luminometer 4 Working with the FB12 PC Software 4 4 FB12 Luminometer Setup Connect the FB12 luminometer with the cable supplied to a serial COM port on your PC L Select NEXT and PC OPER on the main menu to set the FB12 luminometer to PC operation FB12 Luminometer Measure MEAS SET FB12 Luminometer PC Oper SYSTEM PC OPERATION 30 RLU s DOOR OPEN END Q The measuring instrument is now in the PC operation mode The measured values are displayed continuously in the top row The bottom row shows the status of the sample drawer Push END to return to the standalone mode Note The function PC operation is not available if the serial interface has been set to PRINTER To configure the interface for PC operation set the RS232 interface configuration on the SYSTEM menu to PC communication SYSTEM NEXT SYSTEM CONFIG RS232 FUER PC PRINTER SCROLL NEXT Berthold DETECTION SYSTEMS a 47 4 Working with the FB12 PC Software FB12 Luminometer Q Push SCROLL and change the instrument from PRINTER configuration to PC configuration Q Push ENTER to confirm your entry Push MAIN to return to the main menu Q On the main menu select NEXT and then PC OPER which is now available Berthold 48 DETECTION SYSTEMS FB12 Luminometer 4 Working with the FB12 PC Software 4 5 Comparison of Software Protocols To help you get a quick overview
6. here Door close By closing the sample drawer Measurements with injectors can only be started in this manner Number of replicates Advanced Options Click this button to open page 2 of the Single Kinetics Parameters dialog box Use luminometer as time basis Select the luminometer as time basis Berthold 28 DETECTION SYSTEMS FB12 Luminometer 8 Single Kinetics 8 2 Measurements Preparation At luminometer Select NEXT and then PC OPER In the PC software Select a protocol type Single Kinetics click Run and the program will go to the measurement menu Parameter Display of measurement parameters You may type a background value into the Background RLU s box The value entered here is subtracted from the raw data of each sample Measurement start By opening closing the sample drawer or by clicking the Start button Results The measurement values will be displayed in a table depending on the definition in the protocol with the following columns one row for each sample Time Length of time the light emission is measured Rep Measurement values for individual replicates Average Average value of replicates Vostdv Av Standard deviation in percent divided by average value Net Net value average value minus BG value SD Net Standard deviation in percent divided by net average value Graph The measurement data trend of each single measurement can be displayed in a graph either
7. online or at the end of the measurement by clicking the graph button Further options for processing the graph become visible when you enlarge the graph window Group Select which replicate curve s is to be displayed Smoothing Smoothes all displayed curves Log scale Logarithmic scaling RLU range Select if scaling is to be applied automatically or manually Berthold DETECTION SYSTEMS 2809 9 Multiple Kinetics Figure 9 1 Dialog box Multiple Kinetics 30 Parameters Page 1 FB12 Luminometer 9 Multiple Kinetics In the Multiple Kinetics mode the trend of several samples is measured in parallel over a longer period of time 9 1 Protocol Select Multiple Kinetics and push Create Multiple Kinetics Parameters Multiple Kinetics m Time settings Delay time Total duration Sampling time os fiom aos H4 ros se No of data points Interval time ho d moo Lotes Note Interval time Total duration No of data points to nearest 0 25 m Replicates and samples Number of replicates Number of samples f a E z Comments em Excel TM Options Time settings The following 5 parameters of this group box are mutually conditional with the exception of delay time Delay time Delay before every measurement Total duration Total sample measurement time including the following times Sampling time Measurement time per data poin
8. the installation disk 2 with the file FB12Setup WO2 in drive A Upon completion of the installation process click Finish Q The software includes a 30 days trial option for non purchased software components Important information for Windows NT 2000 users Please make sure to install and run the software in the administrator mode If this is not possible you should obtain the req uired user rights from the administrator in order to be able to work with the software Berthold DETECTION SYSTEMS 11 4 Working with the FB12 PC Software FB12 Luminometer Installation of additional protocol types Proceed as described above Select the protocol types you want in the Software Components dialog box Then complete the installation 4 2 First Program Start Q Q Q Double click on the FB12 software ICON Click on the lt Options gt button Select the serial port used COM1 or COM2 in the Serial Port box Set the baud rate to 9600 In the Default Data storage directory box enter the directory where you wish to save the measurement data If you want to hear a signal at the end of a measurement select the item Beep when measurement is completed Click lt OK gt to save the entries The program changes to the Protocol Manager Software Registration Enter the registration passwords one for each protocol type in the Registration Form dialog box 12 Berthold DETECTION SYSTEMS FB12
9. with Additional Options The FB12 luminometer can be upgraded by an external serial printer to output the measured results directly to printer To this end the interface has to be set to PRINTER in the instrument software In this mode cut off measurements can be carried out in addition to extended raw data measurements and their results can be output to printer The serial port is designed for the serial printer Seiko Instruments TM DPU 414 which you can order directly from Berthold Detections Systems 11 1 Physical Installation of the Printer Q The Seiko TM DPU 414 Thermal Printer is supplied with a power supply 7 5V and an interface cable 9 pin data cable male female Q Plug the appropriate plug module into the power supply The power pack adjusts to the supply voltage of 220V or 110V Q The power supply should be properly plugged into the wall outlet while the pin plug is plugged into the connection at the back side of the printer Q The power supply of the printer and the luminometer can be discriminated by the standard device plug 5 5mm and the CINCH plug 3 5mmm respectively Q Connect the enclosed interface cable to the serial ports The female end should be plugged in to the back of the FB12 while the male end should be plugged in to the pack of the printer Berthold BO DETECTION SYSTEMS FB12 Luminometer 11 External Printer with Additional Options 11 2 Printer Setup From the main menu on the FB12
10. Assay click lt Run gt and the program will go to the measurement menu Parameter Display of measurement parameters series A and series B The measured background values are displayed in the Background A and Background B text boxes if the first sample of each sequence was defined as background in the protocol A background value can be entered manually The value displayed here will be subtracted from the raw data of each sample Measurement start By opening closing the sample drawer or by clicking the Start button Results Display of measured values Sample Sample name Net A Net rate of series A average value of the replicates of the first sample of sequence A minus background Net B Net rate of series B A B Calculation of the two net rates according to the formula defined in the protocol VoSD Avg Standard deviation in percent average value Rep A Measurement value for replicates of series A Average A Average value from replicate results of series A of the respective row SD Avg Standard deviation in percent average value Net Average value minus background series A SD Net Standard deviation in percent average value The following columns contain the measured values for series B Berthold PEER DETECTION SYSTEMS FB12 Luminometer Figure 8 1 Dialog box Single Kinetics Parameters Page 1 8 Single Kinetics 8 Single Kinetics The measurement type Single Kinetics i
11. FB12 Single Tube Luminometer User s Manual FB12 e 07 04 D e rt h O d Berthold Detection Systems GmbH 2004 DETECTION SYSTEMS FB12 Luminometer Contents Contents Safety itle fee T 1 1 Getting SA Pee 3 2 Structure of Microprocessor Software cesse 5 3 Measurement in Stand alone Mode eere 7 ll OVENI W centers etuer M PD I ree ee sre eas omen reeves eeu 7 3 2 Raw Data Measurement cccccceececcececceececeeceececcecaecuecueeesaeeeeneeeeas 9 3 3 Check Mode O T nere men terne retra tri terrere rri rei reris 10 4 Working with the FB12 PC Software cesses 11 4 1 PC Software Installation nn 11 4 2 Ears 12 4 3 Basic Software Structure sss nennen nennen enne 13 4 3 1 Protocol MArdOeL uasa caida nn ree 14 4 3 2 Measurement Menu cccccceececeeceeceececeeceeceececeeeaecneeneeneas 15 4 3 3 Working with the Software eene 16 4 4 FB12 Luminometer Setup ccccccccccceeesseeeeeeeeeeeeeeasseeeeeeeeeeseees 17 4 5 Comparison of Software Protocols cceeeeeeeeeeeeeeeeeeeeeeeeeeeeaes 19 5 Quick Measurement 2 c2cceceeceeceececceceeceeceececcecceceeceececeeceeseeseeeeees 20 5 1 I med O10 0 0 enn nmnenne re nnmemse rere tremen re pete pe tense seriis 20 5 2 Measurements sssssssssses
12. R NEXT CROLL MAIN NEX MEASURE 4500 RLU s MEASURE 30 RLU s ARE YOU SURE NEGREPL1 D 0 0s T7 6 5s D 0 0s T 10 0s MAIN i RC aveces cv I HEISE EA E O Minnen YES NO i CROLL CURSOR NEXT CROLL CURSOR OPERATOR usus SCROLL CURSOR NEXT PROTECTED MAIN LANGUAGE SCROLL MAIN ENGLISH NEXT ENTER PASSWORD XXXX SCROLL CURSOR NEXT DONE 4500 RLU s MEASURE 4520 RLU s REMOVE NEGREPL1 MAIN D 0 0s T 6 5s MAIN CUT OFF FACTOR X SCROLL CURSOR NEXT DATE 01 08 2003 09 37 SCROLL CURSOR NEXT CLOSE DOOR MAIN REMOVE SAMPLE MAIN NEG STAND REPL SCROLL MAIN BAUDRATE 9600 SCROLL MAIN NEXT NEXT MEASURING TIME 10 0s MEASURE SCROLL CURSOR MAIN NEGREPL2 D 2 0s T 10 0 SAMPLE REPL SCROLL MAIN 4 NEXT RLU FAKTOR 1 000 SCROLL CURSOR MAIN MEASURE 4500 RLU s NEGREPL2 D 0 0s T 6 5 s DELAY TIME 20s SCROLL CURSOR NEXT DONE 4500 RLU s REMOVE NEGREPL2 MAIN MEASURING TIME 10 0s SCROLL CURSOR MAIN MEAS TIME 10 0s SCROLL CURSOR NEXT INSERT SAMPLE 1 REPL1 CLOSE DOOR MAIN MEASURE 22 RLU s T 10 s MAIN MEASURE 30 RLU s S1R1 D 2 0s T 10 0s DONE REMOVE S1R1 4500 RLU s MAIN Berthold 6 DETECTION SYSTEMS FB12 Luminometer 3 Measurement in Stand alone Mode 3 Measurement in Stand alone Mode 3 4 Overview The luminometer should be switched on at least 30 minutes before starting a mea
13. as been reserved for each replicate If the number of replicates was defined as variable in the protocol you may increase the number of columns by clicking the appropriate button Average This column contains the average values calculated from the replicate results of that row VoSD Avg Standard deviation in percent divided by average value Net Average value minus background value SD Net Standard deviation in percent divided by net average value The measurement data trend of each single measurement with data points can be displayed in a graph either online or at the end of the measurement by clicking the graph button Berthold DETECTION SYSTEMS 23 7 Dual Assay FB12 Luminometer T DualAssay In the measurement mode Dual Assay two sample sequences A and B are measured Subsequently a mathematical correlation is established between both measurement sequences Typical application Dual reporter gene assays 7 1 Protocol Select Dual Assay and press Create Figure 7 1 Dialog box r Series measurement and delay times r Series B measurement and delay times Save Dual Assay Delay time Measurement time Delay time Measurement time Save As Parameters gp 4 Cancel P age 1 Start measurement by C Door close Start button or space bar or enter key r Number of replicates and samples Number of replicates 3 ES imer Number of samples 4 First samp
14. at TM 97 Berthold DETECTION SYSTEMS 43 4 Working with the FB12 PC Software FB12 Luminometer 4 3 1 Protocol Manager Protocol types Shows the protocol types which can be used as templates for creating protocols Runable protocols Shows a list of created protocols Protocols are edited using the buttons in this box Run Shows the measurement menu and you can start a measurement with the selected protocol Edit Edit the parameters in the selected protocol Delete Delete the selected protocol Quick Measurement is not a protocol type but a measurement protocol parameters may be changed but may not be stored in separate protocols Retrieve Shows the stored measurement data double click to open the desired data file MDS Berthold 14 DETECTION SYSTEMS FB12 Luminometer 4 Working with the FB12 PC Software 4 3 2 Measurement Menu Select the desired protocol and click lt Run gt The measurement menu is displayed Before starting a run On the luminometer Select NEXT and then PC OPER see chapter 4 4 Measurement start By opening closing the sample drawer or pressing the green button or the spacebar Meas completed As soon as the measurement time is over Measurement stop By opening the sample drawer or pressing the red button Single values Measured results are transferred from the luminometer to the PC every 0 2 seconds To view single values place the cursor
15. ations on the installation and or operation of sample measuring systems that are required by law The manufacturer guarantees safe operation of the equipment both electrically and mechanically if user follows the instructions set forth in this manual The user must ensure that the instruments are set up and installed in such a way that their function is not impaired The instruments have been tested by the manufacturer and are supplied in a condition that allows safe and reliable operation This User s Manual includes information and warnings that have to be observed by the user to ensure safe operation of the instruments Please adhere to the following safety instructions before taking the instrument into service and during operation of the system 1 Only trained personnel may operate the instrument It is strongly recommended that all users read this manual prior to use 2 The instrument may only be used for the designated application A single sample luminometer is employed for detection of chemi and bioluminescence in different sample tube formats 3 Berthold Detection Systems assumes no liability for any damages including those to third parties caused by improper use or handling of the device 4 The system is designed according to the IEC 1010 1 or EN 610 10 1 regulations for electrical measuring systems 5 Only qualified personnel may carry out Service and repair work Reassemble it completely before use 6 T
16. ctecatenctececenstesenents 12 getting started eese 3 installation zie ent rn D rnekus 3 11 instrument parameters 4 maintenance MOMENTE 42 measurement menu 15 measurement time 7 menu SITUclure eoeo reet 5 microprocessor software ss s 111111 5 multiple kinetics PC software 30 operating elements 5 Overload ognanina 7 PG operation acere erbe puentes ad ena ade i2d 17 Berthold DETECTION SYSTEMS 15 Index PC software adu acieeseceeqnecucacsass saos knsadc 11 IS UT ERR NER RR RERO MS 3 4 36 printer installation 36 printer setup iciissicsesdadaocossai dadas esed utis 37 protocol manager sesse 14 protocol types ssssseeeeeeeeerrnnnner 14 quick measurement PC software 20 raw data measurement 9 41 repeating a measurement 8 jj CS 14 return shipment sees 2 runable protocols 14 safety instructions sssuus 1 Sample formats sneen 3 zig 17 single assay PC software 21 single kinetics PC software 27 software registration 12 software structure 13 16 stand alone mode
17. d Push MAIN to terminate the measurement PC OPER SYSTEM MAIN Q Push the SYSTEM button to open the PARAMETER menu SYSTEM CONFIG CHECK NEXT Q Push the CHECK button The selected measurement time is used as integration time MEAS TIME 10 0s SCROLL CURSOR NEXT Q Change the measurement time with SCROLL and confirm with lt ENTER gt Push NEXT to start the measurement MEASURE 22 RLU s T 10 0s MAIN Q Push MAIN to return to the main menu Berthold DoS DETECTION SYSTEMS FB12 Luminometer 4 Working with the FB12 PC Software 4 Working with the FB12 PC Software 4 1 M Oooo L PC Software Installation Close all Windows applications Insert software CD in the CD drive CD navigator is supposed to automatically start your internet browser Select Software in the navigator bar then FB12 Sirius Software Installation on the respective site Double click the Setup exe file displayed in your Windows Explorer to start the setup program Alternatively if the CD navigator does not automatically pop up chose the Start menu in Windows The Run dialog box appears In the Open text box type x FB12 Software Setup exe x stands for your CD drive This will start the setup program too Click lt Next gt Select Program Manager Group Click Next display of Start Installation Click lt Next gt to start the installation If the prompt Place installation disk2 is displayed insert
18. eceeceeceeceececeeceecesaecaececeeseeaeeeenenees 39 11 5 Extended Raw Data Protocol cccccceececeeceeceececceceeceeceseeeaeenseneeees 40 11 6 Raw Data Measurement 2 ccc ccc eccecceceeceeceeceececeececusceeaeeeeuseneaeenes 41 12 Maintenance scener ces odzoni2iioiiadoiiorclico comic ioicrecou eaten ERE RER 42 13 Technical Data c cccceccecceccecceceececceceeceeceececceceeceeceececeeceeseeeeceeeeees 43 14 Preparing FB12 for Transport eese 45 15 Inde E E eee cocos ccu cec ec eoo vase e oz conus eoi cou cora Du ecc ci ero DD D IUe 47 16 Decontamination Form uses ELLER ERE REE REEL REELLE ES 49 Typographical Conventions FB12 Luminometer Messages on the LC display are printed in boldface type e g menu MEASURE SCROLL or MAIN The lt ENTER gt function key is printed in boldface inside pointed brackets PC software Menu and option titles of the PC software are printed in boldface inside square brackets e g File Options PC software buttons are printed in boldface inside pointed brackets lt gt e g lt OK gt lt Start Quick Measurement gt lt F1 gt lt enter gt lt Alt gt Berthold DETECTION SYSTEMS FB12 Luminometer Safety Instructions Safety Instructions The FB12 luminometer was manufactured in accordance with safety requirements for electronic and medical luminometers It is the operators responsibility to adhere to regul
19. emem eee ener her here tre seite reperiri iiiter rere ERE ER 20 6 Single SSAY cM 21 6 1 Protocol ee 21 6 2 Measurements smsen tt kk kk nenne here rre tii tei LEE rri rii rire reines 23 CEU P o pee 24 7 1 Protocol eode here eea rere ten terere repe a sensores 24 7 2 Measurements smsen ket kk kk Ek E ERE ERE iiiter reperi rri rii perennis 26 EN Slc meer ELLE nnmnnn 27 8 1 Protocol sssessssseseeeenenretne re A re tretretere rere peret sensores 27 8 2 Measurements seere tk k eee reme het nere tre titre reperire rri rer rere reis 29 9 DGunrAG oq 30 9 1 Protocol ssssssesseneemI II II nee men terrere tiere rerit re terrere rii rer riis 30 9 2 Measurements Museer erem en Ek Ek ERR ERE tre rati rere re ris rri repere reis 31 10 Cutoff ASS Beene eee rae eevee ae araea rae are e aRar ana aa aa ERa aaa rary 33 10 1 Protocol 2 02 2 ccc cccceceececcecceceececceececceceeceeceececaececeecescesseceeseeseseeaesseneesees 33 10 2 M ASUrEMeNMS cccccceccecceeeececeeceeceeceeaecuecuececaesaeceeceesesaesaeseeseeesess 35 Berthold DETECTION SYSTEMS Contents FB12 Luminometer 11 External Printer with Additional Options 36 11 1 Physical Installation of the Printer eceee ccce esae 36 T2 alg cet lt 0 0 RR RERO NET 37 11 3 Gut off PIOIOCO erectos uoce eee dec ceese ee eaten eos 37 11 4 Cut off Measurement 0 2 cece cece ccc
20. ents of 0 1s 2 measurement modes Raw Data measurement Cut Off option Standard and Check mode Measurement time 0 99 9s Interfaces Serial RS 232 interface for PC connection Printer External printer option Dimensions W 220 mm D 170 mm H 220 mm approx 2 5 kg e Data transfer via RS 232 interface PC software MS Windows application for control measurement and evaluation Power supply DC 12 V 2 5A peek 1 A average Temperature range Storage 0 40 C Operation 10 35 C idi 10 80 Berthold DETECTION SYSTEMS 43 13 Technical Data FB12 Luminometer Platform Required Windows compatible PC Pentium like processor one available Hardware serial port Operating System Windows 98 ME XP NT 4 0 2000 XP professional Additional Software Microsoft EXCEL 97 2000 XP optional Accessories 18100010 Reflector type RFB201 3 18120010 Power supply unit 12V min 0 8 A input voltage 100 240V 18200250 Serial data cable 13200500 5 ml tubes 12x75 mm 500 each 15300100 External thermo printer Seiko DPU 414 with data cable power supply 12V min 0 8 A input voltage 100 240V Berthold 44 DETECTION SYSTEMS FB12 Luminometer Accessories box place holder Original packaging FB12 Luminometer 14 Preparing FB12 for Transport 14 Preparing FB12 for Transport If it should become necessary that FB12 has to be serviced please observe the following instructions
21. for shipping it to your local distributor or Berthold Detection Systems 1 Turn luminometer off and disconnect power supply Remove the reflector 3 Pack the luminometer into the plastic bag and put it into the white original packing together with the brown placeholder cardboard box the accessories box and the flat place holder Put this in the brown dispatch cardboard box If you return the manual also please put it on top of the white cardboard box 4 Complete the packaging by filling out the box with paper and seal the dispatch cardboard box Please contact service Berthold ds com or your local dealer if you need any packaging material Improper packing may cause damage during transportation 5 Before return please contact your local distributor or Berthold Detection systems for shipping instructions Berthold DETECTION SYSTEMS 45 FB12 Luminometer 15 Index analytes ssssssssssssssse 34 check Mode oet aviae bead ed 10 comparison of software Protocols 19 configuration esserne 4 cut off PC software 33 cut off printout essence 40 cut off protocol stand alone 37 decontamination form 48 delay TITIO iuda db idit Ru t i tasas 7 discarding a measurement 8 dual assay PC software 24 EXCEL transfer 2 15 extended raw data protocol standalone See oe 40 first program start cso sn
22. he user may only perform the maintenance work described in this manual Berthold DETECTION SYSTEMS Safety Instructions FB12 Luminometer 7 Use only parts described in this manual for servicing 8 The instruments are live and improper handling may cause damage 9 If you realize that the instrument has become unsafe to use switch it off and disconnect it from the power supply 10 All instruments supplied and all additional devices must be grounded Use three prong grounded plugs 11 Before opening the locking screw or the instrument head on the top side of the instrument disconnect power and then open the sample drawer to protect the photomultiplier against direct incident light Otherwise the photomultiplier may get damaged Do not keep the sample drawer open for a long period of time 12 If liquid gets inside the instrument pull the power cord Clean the unit or have it cleaned by an authorized service center The tests and maintenance work recommended by the manufacturer should be performed to make sure that the operator remains safe and that the instrument continues to function correctly Authorized service engineers must perform any service and maintenance work not described in the operating manual Return shipment f the instrument has to be returned to Berthold Detection Systems for servicing or inspection we recommend that you use the original cardboard box Please remove the reflector sample holder in
23. le is background Comments Measurement options Transform FE Measurement order B A 8 Advanced Options Excel TM Options Series A measurement and delay time Delay time Delay for sequence A prior to each measurement can be set only if a measurement is started by closing the sample drawer Measurement time for each sample measurement of series A Series B measurement and delay time Enter parameters for series B in the same manner as for series A 24 Berthold DETECTION SYSTEMS FB12 Luminometer 7 Dual Assay Start Meas by Select how to start the measurement Start button By clicking on the Start button or by pressing the spacebar No delay time can be defined here Door close By closing the sample drawer Number of replicates and samples Number of replicates Number of samples First sample is background Measurements of the first sample replicate are considered background measurements Measurement options Transform Select the calculation formula Measurement order Selection of order Either one sample of sequence B after each one of sequence A or all samples of sequence A in a row and then all those of sequence B Berthold DETECTION SYSTEMS 85 7 Dual Assay FB12 Luminometer 7 2 Measurements Preparation At luminometer Select NEXT and then PC OPER In the PC software Select a protocol type Dual
24. nt X Neg Net net rate of negative control Pos Factor any constant X Pos Net net rate of positive control lt Advanced Options gt Click this button to open page 2 of the Cut off Parameters dialog box Result text Designation for Cut off results The following designations are defaulted Negative result text NEG Positive result text POS Equivocal result text Berthold DETECTION SYSTEMS FB12 Luminometer Preparation Parameter Measurement start Results 10 Cut off Assay 10 2 Measurements At luminometer Select NEXT and then PC OPER In the PC software Select a protocol type Cut off click Run and the program will go to the measurement menu Display of measurement parameters You may type a background value into the Background RLU s box The value entered here is subtracted from the raw data of each sample By opening closing the sample drawer or by clicking the Start button Measured values are displayed in a table Sample Sample name Rep A column is reserved for each replicate Result Result column POS NEG or as result Average Average value from the replicates of one sample SD Avg Standard deviation in percent average value Net Average value minus background value SD Net Standard deviation in percent net average value Berthold DETECTION SYSTEMS 35 11 External Printer with Additional Options FB12 Luminometer 11 External Printer
25. of the different protocol types we have summarized their functions in the table below oS Single Single Multiple Dual Cut Off SS Assay Kinetics Kinetics Assay Assay Measurement of Continuous Several samples Measurement of Classification of several samples measurement of discontinuous sample series A samples with replicates one sample measurement no no BG may be BG may be defined defined 1 sample BG Number of samples Replicates flexible yes same number yes for for A and B samples neg and pos controls flexible 1 sample any with any data same number yes for measured with i for A and B samples any data points neg and pos controls Calculations none 71 replicate 21 replicate 71 replicate 71 replicate 71 replicate average value average value average value average value average value stdv av stdv av stdv av stdv av stdv av repl 1 repl 2 repl 1 repl 2 log normal log normal scaling scaling smoothing smoothing peak half life time peak half life time during increase during increase and decay and decay Kinetics graph none E for last complete graph of complete graph of always for last always for last E all replicates all replicates sample sample Miscellaneous Meas predefined measurement predefined predefined sequence sample names start only at sample names sample names closing of sample M P different different cut off drawer calculation calculation
26. on last line to add a sample Grid Options You may select a flexible table set up so that the number of replicates and or samples may be changed during measurement at the push of a button Measurement orders Order of measurement Across Sample Rep1 Repz Measurements are carried out by rows first all replicates of the first sample then all those of the second sample etc Berthold DETECTION SYSTEMS FB12 Luminometer Preparation Parameter Measurement start Results Graph 6 Single Assay Down Measurements are done by columns e g first the first replicate of all samples then the second replicate of all samples etc 6 2 Measurements At luminometer Select NEXT and then PC OPER In the PC software Select a protocol type Single Assay click Run and the program will go to the measurement menu Display of the run parameters If you have defined the number of replicates as variable you may change this by clicking on the respective button A background value can also be set manually if no background measurement is carried out The value entered here is subtracted from the raw data of each sample By opening closing the sample drawer or by clicking on the Start button The measurement values will be displayed in a table depending on the definition in the protocol with the following columns one row for each sample Sample Sample name Rep One column h
27. order to avoid damage to the sample chamber see chapter 14 Berthold 2 DETECTION SYSTEMS FB12 Luminometer 1 Getting Started 1 Getting Started Description The FB12 luminometer is a highly sensitive and compact single sample luminometer designed specifically for the detection of chemiluminescence and bioluminescence in different kinds of applications It can be employed for all glow type measurements The sample chamber accommodates various sample formats FB12 may be operated as a standalone instrument using special Windows software Optionally an external printer can be connected to the FB12 In this case additional cut off measurements can be run on the standalone instrument and the results can be output to printer see chapter 11 Luminometer installation Q The instrument should be set up in a dry dust free environment and protected from direct sunlight and significant temperature fluctuations Q Carefully unpack the FB12 Luminometer and put it on your desktop Q Plug the connector module that fits into your wall outlet onto the power supply unit B Q Plug the power supply unit into a wall outlet and the pin F connector of the power supply unit into the socket on the instrument rear panel Q Turn the instrument on at the mains switch The display will show Please wait while the instrument is performing a self test Then the main menu appears Q Open the sample drawer and insert the sam
28. over a measured result Result fields To change the size of a result field move cursor to the right hand margin of the header As soon as the cursor shape changes to a double arrow click the left mouse button and drag the cursor with the mouse button held down left or right EXCEL Transfer Click the button depicted to the left to manually import data into a new or predefined Excel worksheet The transfer may also be automated in the protocol Berthold DETECTION SYSTEMS LL 45 4 Working with the FB12 PC Software 16 m ee p je M E FB12 Luminometer 4 3 3 Working with the Software Tool buttons on the measurement menu simplify program handling Apart from a few exceptions you need not select any pull down menus Tool buttons and their meaning Meaning Save measurement data Import measurement data into Excel Print Start measurement Stop measurement Delete last measurement Display results in spreadsheet Display results in a graph Tile window horizontally Tile window vertically Create new data file in measurement menu Copy selected data to clipboard Pull down menu gt Option File gt Save as Edit gt Send to Excel File gt Print Measure gt Start Measurement Measure gt Stop Measurement Measure gt Measurement Delete Last Measure gt Kinetics Measure gt Kinetics Window gt Tile Horizontal
29. phone Date Signature Please copy and fax to Berthold Detection Systems Bleichstr 56 68 75173 Pforzheim Tel 49 0 7231 9206 0 Fax 50 Berthold DETECTION SYSTEMS 49
30. ple holder reflector Different sample formats may be used Culture dishes with up to 35 mm diameter Scintillation bottles containing up to 20 ml Reaction bottles with up to 12 mm diameter and up to 75 mm length see chapter 13 Berthold DETECTION SYSTEMS 1 Getting Started Figure 1 FB12 Luminomete r rear view FB12 Luminometer ON OFF switch Serial port Socket Configuration Set up the instrument parameters according to your needs as follows confirm entries with Enter push Next to continue with next display FB12 LUMINOMETER MEASURE MEAS SET PC OPER SYSTEM MAIN USE RS232 FOR PC PRINTER SCROLL MAIN NEXT Qn Select PC if the FB12 is used as standalone instrument for raw data measurements or if the luminometer is connected to a PC Q If you are using the FB12 as standalone instrument to run cut off measurements or you want to print out raw data then connect an external printer and select PRINTER see chapter 11 Q Then select the desired language and enter Date and Time Baudrate 9600 and the RLU factor 1 Berthold DETECTION SYSTEMS FB12 Luminometer 2 Figure 2 Operating elements on front panel MEASURE MEAS SET PC OPER SYSTEM Accept Input 2 Structure of Microprocessor Software Structure of Microprocessor Software Operating elements on the front panel Berthold DETECTION SYSTEMS Main menu
31. press the NEXT button and go into SYSTEM FB12 LUMINOMETER V2 2 MEASURE MEAS SET NEXT Vy PC OPER SYSTEM NEXT Vy SYSTEM CONFIG CHECK NEXT Vy RS232 FOR PC PRINTER SCROLL MAIN NEXT L Press SCROLL to adjust the instrument from PC configuration to the printer configuration Confirm your entry with ENTER I Push MAIN to return to the main menu 11 3 Cut Off Protocol Provided the interface is set to PRINTER you can choose either cut off protocol or extended raw data protocol by pushing MEAS SET on the main menu see chapter 11 5 and define the respective protocols FB12 LUMINOMETER V3 0 MEASURE MEAS SET NEXT Vy PROTOCOL RD CUT OFF SCROLL MAIN NEXT Berthold DETECTION sYsTEMS 87 11 External Printer with Additional Options FB12 Luminometer L Push SCROLL to select the cut off protocol OPERATOR SCROLL CURSOR NEXT AVERSAMNVAIE SEES UD SCROLL CURSOR NEXT Enter a user name and a test name and then define the cut off factor the number of negative standard replicates the sample replicates the delay time and the measuring time CUT OFF FAKTOR X XXX SCROLL CURSOR NEXT Vy NEG STAND REPL 2 SCROLL CURSOR NEXT Vy SAMPLE REPL 1 SCROLL CURSOR NEXT Vy DELAY TIME 4 0 SCROLL CURSOR NEXT Vy MEASURING TIME 40 0 amp SCROLL CURSOR NEXT Berthold 38 o DETECTION SYSTEMS FB12 Luminometer 11 External Printer with Additional Options 11 4 Cut off Measurement The CUT OFF
32. protocol will allow replicates to be measured and classified as positive or negative samples This is done by calculating a Cut off threshold Based on the average of the negative standard replicates and the Cut off factor this threshold is calculated as follows Ned STD Replicates Average x Cutoff Factor Cutoff threshold The RLU value of the negative standard replicates are measured at the beginning of the measurement sequence From there the Cutoff threshold is calculated If the interface has been set to PRINTER and you have selected the cut off protocol after you have pushed MEAS SET you can run a cut off measurement by selecting MEASURE on the main menu Q Select menu MEASURE Q The Cut off measurement menu starts Place tubes for negative standard replicate 1 and close sample drawer to start the measurement sequence Open sample drawer at the end of the measurement and remove sample tube Q Proceed as instructed on the display insert sample tube for gm negative standard replicate and close sample drawer Average value and standard deviation are calculated and printed out after completion of all standard measurements You are then prompted to insert the first sample replicate Q Close sample drawer The measured values as well as the result of the sample classification are printed out at the end of the measurement Q Open sample drawer and replace sample tube Following this protocol you can measu
33. rage value minus BG value Berthold DETECTION sYsTEMS 91 9 Multiple Kinetics Graph 32 FB12 Luminometer SD Net Standard deviation in percent divided by net average value S2 Rep1 Measurement value of the first replicate of the second sample S2 Rep2 Measurement value of the second replicate of the second sample Average Average value of replicates of the second sample SD avg Standard deviation in percent divided by average value Net Net value average value minus BG value SD Net Standard deviation in percent divided by net average value S Rep Further samples with replicates The measurement data trend of each single measurement can be displayed in a graph either online or at the end of the measurement by clicking the graph button Further options for processing the graph become visible when you enlarge the graph window Berthold DETECTION SYSTEMS FB12 Luminometer Figure 10 1 Dialog box Cut off Parameters Page 1 10 Cut off Assay 10 Cut off Assay A Cut off protocol classifies samples into three categories So called cut off thresholds high and low are measured by defining fixed parameters and measuring negative and positive controls 10 1 Protocol Select Cut off ASSAY and press lt Create gt Cut off Assay Parameters Cut off Assay x r Measure and delay times Delay time Measurement time ros ios Save As r Start mea
34. re as many samples as you choose Berthold DETECTION SYSTEMS 39 11 External Printer with Additional Options FB12 Luminometer Printout to external printer Example Date XX XX XXXX XX XX Name CUT OFF Assay Neg STD Repl 3 Sample replicate 1 Delay Time 2 0s Meas Time 5 0s Cut off factor 2 000 Neg STD Repl Result 1 415 RLU s 2 435 RLU s 3 410 RLU s Neg Standard Avg 420 RLU s AVC 2 6 Cut Off Value 840 RLU s Then the samples to be classified may be measured Based on the calculated Cut Off value of e g 840 RLU s any measurement results below 840 RLU s will be denoted as NEGATIVE while any value above 840 RLU s will be denoted POSITIVE 11 5 Extended Raw Data Protocol With the interface set to PRINTER select the raw data protocol on the main menu as follows FB12 LUMINOMETER V3 0 MEASURE MEAS SET NEXT Vy PROTOKOLL RD CUT OFF SCROLL MAIN NEXT Push SCROLL to select the raw data protocol In this case the raw data protocol is extended you may enter a user name and a test name in addition Berthold 40 DETECTION SYSTEMS FB12 Luminometer 11 External Printer with Additional Options 11 6 Raw Data Measurement A raw data measurement is started just like a cut off measurement by selecting the MEASURE menu The current measured value is printed out without any further modification or calculation In addition the measurement parameters you have defined the date as well a
35. s and formulas for A classifications and B BG Background Berthold DETECTION SYSTEMS DF 5 Quick Measurement FB12 Luminometer 5 Quick Measurement This protocol type measures the raw data of consecutively numbered samples 5 1 Protocol Q In the Protocol Manager select the Quick Measurement protocol and click lt Edit gt The Quick Measurement Parameters dialog box appears Q Set Delay time for the measurement and Measurement time by clicking on the respective arrow buttons L Click Save to save the measurement parameters The program returns to the Protocol Manager 5 2 Measurements Q In the Protocol Manager select the Quick Measurement protocol and click Run The measurement menu appears showing the parameters Measurement time and Delay time defined for the measurement Q Open sample drawer and insert sample tube Q Close sample drawer The measurement process starts as soon as the preset delay time is over L The result is displayed at the end of the measurement L Proceed in the same manner to measure the next sample Berthold 20 DETECTION SYSTEMS FB12 Luminometer Figure 6 1 Dialog box Single Assay Parameters Page 1 6 Single Assay 6 Single Assay This protocol type allows you to run background measurements and to measure replicates of samples 6 1 Protocol Select Single Assay and push Create Single Assay Parameters Single Assay x m Mea
36. s the entered user and test name will be documented Berthold DETECTION SYSTEMS 41 12 Maintenance FB12 Luminometer 12 Maintenance The FB12 luminometer is practically maintenance free It only has to be protected from dirt and may have to be cleaned The surface of the instrument is protected by a robust and washable finish Should it become dirty or dusty we recommend wiping it with a damp cloth If necessary a mild detergent not an abrasive can be used The sample drawer and especially the measurement window also have to be kept clean and should be wiped off with a damp cloth if dirty Please keep in mind that the screw on top of the instrument must always be firmly closed The PMT may get damaged by incidence of light VAN No fluid should ever enter the instrument If this should happen disconnect the instrument immediately and notify a service representative Berthold 42 DETECTION SYSTEMS FB12 Luminometer 13 Technical Data 13 Technical Data Sample format e Sample tubes with up to 12 mm diameter 12 mm standard and up to 75 mm length with or without cap Culture dishes with max 35 mm diameter Scintillation vials with up to 20 ml content Reaction containers Better than 1 000 luciferase molecules in one reporter gene assay ATP detection is only dependent on the reagents used Microprocessor e software 3 softkeys and 1 ENTER button Delay and measurement time definition in increm
37. s used to measure the trend of the light emission over a specific period of time 8 1 Protocol Select Single Kinetics and push Create Single Kinetics Parameters Single Kinetics r Time settings Delay time Total duration Sampling time r os fim H4 1 0 Save As No of data points Interval time 20 3s Cancel Note Interval time Total duration No of data points to nearest 0 2s Start timer by Door close Start button or space bar or enter key No of replicates Comment Advanced Options Excel TM Options Time settings The following 5 parameters of this group box are mutually conditional with the exception of delay time Delay time Delay before each measurement can be set only if a measurement is started by closing the sample drawer Total duration Total sample measurement time including the following times Sampling time Measurement time per data point Berthold DETECTION SYSTEMS OF 8 Single Kinetics FB12 Luminometer No of Data points Number of data points multiplied by the interval time shows the total duration of the measurement Interval time The interval time may be longer than the individual measurement time of the data point Start Meas by Select how measurement is to be started on the measurement menu Start button By clicking the Start button or pressing the spacebar No delay time can be defined
38. sure and delay times Delay time Measurement time 2 0 5 0s Save As Start measurement by Cancel C Start button or space bar Door close Loc or enter key m Number of replicates and samples Number of replicates Number of samples 5 fio First sample is background Comments Advanced ptions Excel TM Options Delay time Delay before measurement can be set only if a measurement is started by closing the sample drawer Measurement time Measurement time for each sample measurement Start Meas by Select how to start the measurement on the measurement menu Start button By clicking the Start button or pressing the spacebar No delay time can be defined here Berthold DETECTION SYSTEMS LS 94 6 Single Assay Figure 6 2 Dialog box Single Assay Parameters Page 2 22 FB12 Luminometer Door close By closing the sample drawer of the luminometer Number of replicates Number of samples First sample is background Measurements of the first sample replicate are considered background measurements Advanced Options Opens page 2 of the Single Assay Parameters dialog box Single Assay Advanced Options Single Assay x Grid options Flexible number of samples Cancel Measurement entry order Across C Down Pre defined sample names Sample Hame Press Tab or Enter
39. surement Units Luminescence is measured in Relative Light Units RLU The RLU s displayed by the FB12 luminometer are equal to the counts s picked up by the detector with the default RLU factor set to 1 RLU s cps 8RLU factor The factory set factor 1 should be changed only when comparing results measured with different luminometers The entered factor multiplies all measured results it is changed on the main menu under SYSTEM Overload If one or several measured values in the measurement interval exceed the measuring range of the luminometer OVERLOAD appears on the display To obtain a meaningful result the sample has to be diluted and measured again DONE OVERLOAD RLU s REMOVE SAMPLE MAIN Definition of measurement and delay time Figure 3 Illustration of delay and measurement times measurement time delay time Close sample drawer Time axis Seconds All delay times start with the closing of the sample drawer The minimum delay time is 1 s Berthold DETECTION SYSTEMS 3 Measurement in Stand alone Mode FB12 Luminometer Discarding and repeating a measurement Opening the sample drawer will terminate a measurement In the raw data mode the display shows MEAS ABORTED PRESS ENTER Close the sample drawer and push ENTER to start the next measurement In case of a cut off measurement only possible if the interface has been set to PRINTER the following display appears
40. surement by Cancel C Start button or space Door close bar or enter key Analytes Negative Positive controls Replicates Samples Negative replicates Positive replicates e E mb ux 3 First sample is background r Cut off calculations Offset Neg Factor Pos Factor Low cut off o 1 00 Neg Net 0 00 Pos Net Dffset Neg Factor Pos Factor High cut off o 0 00 Neg Net 1 00 Pos Net Comments zl Advanced ptions Excel TM Options Delay time Delay time before measurement can be set only if measurement is started by closing the sample drawer Measur time Measurement time for each sample measurement Start Meas by Select how to start the measurement on the measurement menu Berthold DETECTION SYSTEMS 3 10 Cut off Assay 34 FB12 Luminometer Start button By clicking the Start button or pressing the spacebar No delay time can be defined here Door close By closing the sample drawer of the luminometer Analytes Define the number of replicates and samples Negative Positive controls Define the number of replicates of negative and positive controls First sample is background Measurements of the first sample replicates are treated as background measurements Cut off calculations Definition of low and high threshold by the following parameters Offset Offset RLU value Neg Factor any consta
41. t Berthold DETECTION SYSTEMS FB12 Luminometer 9 Multiple Kinetics No of Data points Number of data points multiplied by the interval time shows the total duration of the measurement Interval time The interval time may be longer than the respective measurement time of the data point Number of replicates samples The number of replicates or samples Please note A measurement can only be started by closing the sample drawer 9 2 Measurements Preparation At luminometer Select NEXT and then PC OPER In the PC software Select a protocol type Multiple Kinetics click Run and the program will go to the measurement menu Parameter Display of measurement parameters You may type a background value into the Background RLU s box The value entered here is subtracted from the raw data of each sample Measurement start By opening closing the sample drawer or by clicking the Start button Results Measured values are displayed in a table Time Length of time over which the light emission is measured The number of data points in time is calculated by dividing the total measurement duration by the interval time S1 Rep1 Measurement value of the first replicate of the first sample S1 Rep2 Measurement value of the second replicate of the first sample Average Average value of replicates of the first sample SD Avg Standard deviation in percent divided by average value Net Net value ave
Download Pdf Manuals
Related Search