41091008 BlueNotes for M410 Operator Manual Issue 2
Contents
1. 410 91 008 Issue 2 22 October 2008 ECN 543 7 2 5 2 5 1 410 BlueNotesm Software Supervisor Setup Sr BlueNotes 410 Supervisor MASTER File Supervisor Help Qsrerwood BLUeNotes n Start Page To apply Supervisor restrictions start the 410 BlueNotes Software by clicking on the Sherwood Logo Icon From the BlueNotes Start Page select Supervisor from the top menu bar to gain access to the following menu structure then select the Access Option Supervisor Access 410 BlueNotes Software has the facility to enable the set up of System Configuration Calibration Procedures and Analysis Methods to be limited to a designated Supervisor This password protected facility may be set up through the Supervisor Access menu If you wish to apply supervisor restrictions enter a password confirm by re entering and then click Start in Supervisor Mode To apply this new access facility exit the BlueNotes Software from the Start Up page click on file then exit then restart by clicking on the 410 BlueNotes icon Sherwood Logo You will then be asked to enter your password TEE J r BlueNotes 410 Supervisor Access Supervisor Name EMANE Enter new password Re enter new password Start in Supervisor mode 410 91 008 Issue 2 22 October 2008 ECN 543 8 2 5 2 2 5 2 1 2 5 2 2 2 5 2 3 410 BlueNotesm Software Options Connections Within Supervisor Options2. Top Level Menu Structure Top Level 410 BlueNotes A 10 41 410 amp 860 Supervisor 410 amp 805 amp 860 Access Word document User Guide PDF Document Release Notes BlueNotes Presentation 410 91 008 Issue 2 22 October 2008 ECN 543 10 410 BlueNotesm Software 3 2 New Configuration Menu System Configuration Show Configuration New Configuration Configuration Identity Name Originator Memo Sample Introduction Manual Autosampler Dwell Wait Post Standard Rinse Diluter None Red Tube 1 50 Delay Orange Tube 1 200 Delay Peak Selection Manual Automatic Peak Timeout Instrument Warm Up Minutes Readings Units ppm mg l mmol l meq Configuration Locked Delete Configuration 410 91 008 Issue 2 22 October 2008 ECN 543 11 410 BlueNotesm Software 3 3 New Procedure Menu 410 91 008 Calibration Procedures Show Procedure Procedure Identity Name Originator Memo Configuration choose from dropdown list Standard Details Analyte select Format select Max Conc enter value No of Standards enter put standard values in the table which appears when the number of standards is entered Procedure Locked Operate Procedure Delete Procedure Issue 2 22 October 2008 ECN 543 12 410 BlueNotesm Software 3 4 New Method Menu Analysis Methods Show Method New Method Method
3. 410 BlueNotes Software is supplied on a CDROM The user requires the 16 character alphanumeric registration code or activation key in order to access the full software package which runs in Windows XP Pro SP2 Principles of Operation Once the 410 BlueNotes Software has been installed on a PC it requires the following information to operate e The COM Port address es of the 410 Digital Interface and the Model 860 Autosampler if applicable e A System Configuration file to indicate sample introduction mode Manual or Autosampler Model 805 Diluter use and dilution ratio or not signal selection method measurement units and analyte More than one configuration file may be stored e A Calibration Procedure linked to a configuration file including Calibrator details required to create a calibration graph e An Analysis Method linked to a calibration procedure including sample details and Spot Check standard options NOTE The serial number of the 410 Digital Interface to which the software is connected must match that within the software being utilised otherwise the two will not communicate Software installation The 410 BlueNotes Software is supplied on a CD ROM with a unique 16 character alphanumeric registration code Insert the CD ROM into the disk drive of a PC The software should auto run and the following window appear BlueNotes 410 Installation Blue Notes m Welcome to the BlueNotes 410 Installer
4. contact for all issues of supply and operation should be sent to info sherwood scientific com Tel 44 0 1223 243444 Fax 44 0 1223 243300 410 91 008 Issue 2 22 October 2008 ECN 543 3 410 BlueNotesm Software 1 2 Using this Manual This Operator Manual will inform the user how to operate the 410 BlueNotes Software in conjunction with a Model 410 Flame Photometer fitted with a Digital Interface and where appropriate the Model 805 Diluter and or the Model 860 Autosampler The user will be able to Set up system configurations e g Model 410 Flame Photometer only amp manual sample input or Model 410 Flame Photometer with Model 860 Autosampler Assign Supervisors and Users Create new Calibration Procedures single or multipoint and new Analysis Methods Assign the use of Spot Check standards and implement Auto Correction facility Perform Calibration Procedures and run Analysis Methods Recall review and reuse previous calibrations Recall and review analyses Automatically generate Analysis Reports Dependent on the system configuration the user may be required to refer to the following additional operating manuals Model 410 Flame Photometer Digital Interface for M410 Model 805 Diluter Model 860 Autosampler 1 3 Warranty The Sherwood Scientific warranty statement is included at Appendix A 410 91 008 Issue 2 22 October 2008 ECN 543 4 2 2 2 3 410 BlueNotesm Software Installation Location
5. Commence installation Bienvenue cu programme d installation BlueNotes 410 Commence installation Click on the required button for English or French installation gt BlueNotes 410 Installation sherwood EE ES Installing BlueNotes 410 Click on Install BlueNotes 410 to load the software onto a P C e AIO F1 L se pointer over button to review the bitfon s action User Guide Word Once the software has loaded the User Guide Adote A same screen will appear again Click on Back to Main Screen Then Ei click on Exit Install M410 Digtal Terminal Back to Main Menu 410 91 008 Issue 2 22 October 2008 ECN 543 5 410 BlueNotesm Software Locate the BlueNotes 410 icon on BlueNotes 410 the PC s desktop and open the application by placing the mouse pointer on the icon and clicking Gepoenpogerge Licence Activation Licenced for unrestricted use with Module A Serial No 21013 The Licence activation page will then appear with prompts to enter the activation key supplied with the I software disc 16 alpha numeric character code Enter Activation Key supplied with CD BlueNotes 410 With the correct code entered the following dialog box will appear Click on OK and the 410 BlueNotes Start Page will appear Place the mouse pointer on Supervisor in the menu bar at the top of the screen select Access and then type letmein in the Enter Supervisor Password bo
6. D ZS S 86040na on 18 Jan 07 at 13 20 86040ne on 18 Jan 07 at 12 46 w a S w s 5 D D S a S Instrument Reading D 8 S Delete Results S S a g o D 5 10 15 20 2 30 35 40 Concentration Multi point Readings Correlation Coefficient 0 99978 Click on the required file to view the calibration graph or select one of the other options shown Back Delete Results or Close 410 91 008 Issue 2 22 October 2008 ECN 543 26 410 BlueNotesm Software 6 2 Analysis Results From the BlueNotes Start Page select Analysis Results using the mouse pointer and click to reveal the Analysis Results page with all the stored analysis files listed an example is shown below ST BlueNotes 410 Supervisor MASTER egen Blue Notes Data Selector Analysis Results 860410 a 86041 Ge 7 860na40 arablab arablabdemo ASSoloDemo bodycote FebNa isdemo jc860test jcmpna860test KMIdilRspe oer eee Method Method2 Method3 You now have a choice click on the required file to highlight it and then show results or Close to return to Start Page If you choose show results a screen like the one shown below appears highlight the required file by clicking on it and then choose one of the option buttons G sherwood Blue Notes m Data Selector Click on Results to View G0apstest on 16 Jan 0
7. Note these will be in descending order from 40 with the first standard in position S on the carousel Procedure Locked Click on this box to prevent changes to the procedure Supervisors click on the Procedure Locked box to grey out user entered variables Alterations to the procedure by regular Users will not be allowed Supervisors can however unlock and make changes Save Click the Save button to save the Calibration Procedure under the name in Procedure Identity The Procedure can then be recalled and used in an Analysis Method 4 2 3 Delete Method Clicking delete Method will prompt BlueNotes to ask the user to confirm before deleting the analysis method 410 91 008 Issue 2 22 October 2008 ECN 543 18 410 BlueNotesm Software 4 3 Analysis Method From the BlueNotes Start Page select Analysis Methods There is a choice Show an existing Method New create Method Operate Method Delete Method Close to return to Start Page If New Method option is greyed out you need to login go to BlueNotes start page and select Supervisor Access from top level menu 4 3 1 Show Method Show Method allows the user to review an existing Method any changes made will prompt BlueNotes to note that the Method details have been modified and ask if the changes should be saved If a Supervisor has locked a method then a regular User will see the greyed out details and no changes can be made If a Supervisor clicks to s
8. Flood lightning earthquake tornado hurricane or any other natural or man made disaster b Fire bombing armed conflict malicious mischief or sprinkler damage c Physical abuse misuse sabotage or electrical surge d Damage incurred in moving the product to another location User agrees to permit Sherwood personnel or personnel of its authorised distributor to make changes in the product which do not affect results obtained but do improve product reliability 410 91 008 Issue 2 22 October 2008 ECN 543 28 410 BlueNotesm Software Product Warranty Statement continued Representations and warranties purporting to be on behalf of Sherwood made by any person including distributors and representatives of Sherwood which are inconsistent or in conflict with the terms of this warranty including but not limited to the limitations of the liability of Sherwood as set forth above shall not be binding upon Sherwood unless reduced to writing and approved by an officer of Sherwood Scientific Ltd Except for the obligations specifically set forth in this warranty statement in no event shall Sherwood be liable for any direct indirect special incidental or consequential damages whether based on contract tort or any other legal theory and whether advised of the possibility of such damages Neither Sherwood nor any of its third party suppliers makes any other warranty of any kind whether expressed or implied with respect to Sherwood Pr
9. Sample 5 450 41 80 40 05 Over range 14 59 03 Sample Sample 6 345 32 02 30 71 14 59 07 Spot Check 20 00 230 21 32 14 59 10 Sample Sample 7 345 32 02 30 04 14 59 13 Sample Sample 8 450 41 80 39 21 Over range 14 59 19 Spot Check 20 00 235 21 78 14 59 22 Sample Sample 9 450 41 80 38 38 Over range 14 59 24 Sample Sample 10 455 42 26 38 80 Over range 14 59 27 Spot Check 20 00 230 21 32 14 59 35 Blank 000 0 00 End 410 91 008 Issue 2 22 October 2008 ECN 543 25 410 BlueNotesm Software 6 File Archives 6 1 Calibration Results From the BlueNotes Start Page select Calibration Results using the mouse pointer and click to reveal the Calibration Results page with all the stored calibration files listed an example is shown below GQ srerwood BlueNotes Data Selector Calibration Results 860410 SE 860410spc Show Results ahmed asmpcNa ASsolodemo Cal4100ne CalPro2 CalProcl CalProc2 demo isdemocal KMIdilRspe MPKMPP GE MPNaMPP mppdilrspe Naappspe NaMidilOsp NaMispc NaMppdilds NaMppspe NappdilOsp You now have a choice Show Results or Close to return to Start Page select a calibration file using the mouse pointer and click All the calibration result files associated with the chosen procedure will appear in date and time order e Configuration Procedure Calibration Method deeg Blue Notes m 1 Data Selector i 86040na on 18 Jan 07 at 13 20 Click on Results to View
10. cause subsequent blank readings to drop slightly will still not send a negative reading to BlueNotes When the displayed reading becomes stable press the Print button on the 410 Digital Interface The reading will be transferred to the calibration table Top Standard no Autosampler Next BlueNotes prompts you to aspirate your top standard Introduce this to the sample uptake tube and wait for the signal to stabilise Adjust the reading if necessary to your required level using the fine control and then press the print button The blank and the fine controls on the M410 must now be left alone If it was necessary to change the course gain setting on the M410 to achieve your desired reading for your standard the calibration process must be done again as the blank reading will not be relevant Subsequent standards no Autosampler For subsequent standards in a multipoint calibration aspirate the standards as instructed by BlueNotes e f using a configuration that specified Manual Peak Selection you need to press print when each standard has achieved a stable reading e f Automatic Peak Selection was specified in the system configuration once the blank and top standards have been set the print button must be pressed immediately the standard is aspirated The software will then count down the Delay then establish when a stable signal is obtained and enter the reading for the standard in the calibration table Continue to follow th
11. exiting one sample and entering the next sample the gap between samples When setting up a method using the Autosampler some experimentation will normally be required to establish appropriate Dwell and Wait times depending on the use of the Model 805 Diluter and sample characteristics Selecting Autosampler enables the Post Standard Rinse feature to become available Selecting Post Standard Rinse instructs the Model 860 Autosampler to rinse the probe and tube briefly in the blank solution every time it leaves the standard in position S the highest or spot check standard No data is collected from this rinse solution but the rinses are shown in the sample table Diluter Indicate here if a Model 805 Diluter is in use If it is the button selected indicates the dilution ratio used the Red tube is for a 1 50 dilution and the Orange tube is for a 1 200 dilution relates to the tubes used on the Diluter The Delay time relates to the time the 410 BlueNotes Software waits before it begins to monitor for a stable signal and is particularly important if Automatic Peak Selection is requested so that the software then determines when to take the reading from the Flame Photometer for standards and samples see below When setting up a new procedure it is usual to perform a certain amount of experimentation to determine the best Delay time set too short and the signal from the detector of the Model 410 Flame Photometer will be climbing
12. have been modified Save changes If a supervisor has locked a configuration then a regular User will see the greyed out details and no changes can be made If a supervisor clicks to show a configuration they have already locked then they will be able to unlock the configuration here 4 1 2 New Configuration To set up a new configuration click to reveal the following gt BlueNotes 410 Supervisor MASTER File Supervisor Help sood BLUENOtes m Configuration Created 17 10 2008 10 58 Modified 17 10 2008 10 58 Memo Default Template Sample Introduction Manual O Autosampler Configuration Locked Instrument Connection Status A Sianal Chart Recorder Clear Freeze Scale 2000 Tme 10 410 91 008 Issue 2 22 October 2008 ECN 543 14 410 BlueNotesm Software Configuration Identity Enter a new Name indicate the Originator of the new Configuration and record any required comments in the Memo text box Note date and time created modified are shown but cannot be altered Sample Introduction Select Manual or Autosampler by clicking the appropriate button If you are using a Model 410 Flame Photometer with a Model 860 Autosampler select the Autosampler option The Dwell and Wait times become active and allow you to set them as required Dwell sets the time the autosampler probe will be immersed within suck up a sample Wait sets the time period between the probe
13. the user must indicate which COM BlueNotes 410 Supervisor Options port the 410 Digital Interface is Connections Display Users Sherwaod connected to or if keyboard entry of data is required If a Model 860 EEN Fl Enabled Autosampler is to be used enable 1 Coupen EE it here and select the appropriate RH PC COM port The 410 Digital Seriaino 1013 Final blank 1 2 Min Interface serial number shown EI Demo Autosampler O Keyboard entry here must correspond to the Digital Interface in the Model 410 Flame Photometer If the serial number is not correct for the interface the 410 BlueNotes Software will not work Note If you are unsure which COM ports the 410 Digital Interface and or Autosampler is connected to with the cable s connected to the PC and the Flame Photometer and Autosampler switched on click on START Microsoft Windows XP then Control Panel then System then Hardware then Device Manager and then open up Ports The COM port connections should be visible there 410 Interface Serial No To determine this if necessary with the Interface connected to a PC serial port access HyperTerminal use these settings 9600 baud 8 bit No parity Stop bit 1 Flow Control None Then switch on the Model 410 the serial number will appear on the PC Screen HyperTerminal is available in Windows operating software packages prior to Vista To access HyperTerminal and set it up to co
14. 410 BlueNotes Software Operator Manual Copyright 2008 Sherwood Scientific Ltd All rights reserved 410 91 008 Issue 2 22 October 2008 ECN 543 1 GADOCUMENT CONTROL Manuals Flame 410 41091008 BlueNotes for M410 Operator Manual Issue 2 doc 410 BlueNotesm Software Contents 1 INTRODUCTION E 3 UN eu Le 3 1 2 Using this MANUAL scissidseidasedesadapadseidanedezadapadessdapedesagapadenssasadesagapadenssasadaeapapadiiess 4 13 Waraniyi eee bei eid 4 2 INSTALLATION E 5 2A Eo e S 2 stetegr e ter geed r selen d ee ede deefe egene ee E eeneg 5 2 2 Principles of Operation 5 2 3 Software Installation cece eee eee eee eee eee eee aaeeeeeeeeeeeeeaaaeeeeeeeeenee 5 2 4 Connecting Peripheral Devices eect ee eeeeccaeeeeeeeeeteeecaaaeeeeeeeeeneee 7 2 4 1 Digital Interface AA 7 2 4 2 M860 Autosampler cccceeceeee cece ee eeeeecaaeeeeeeeeeeeeeaaaeeeeeeeeesteesaaaeeeeees 7 2 4 3 Model M805 Diluter ee eeeccceeeee cece eeeecceeeeeeeeeeeeeceaaaeeeeeeeeeeeeenaaeeeeeees 7 25 SUPEMNISOr SClUD EE 8 2 5 1 Supervisor Access 8 2 5 2 e e EE 8 3 SOFTWARE STRUCTURE grresieg eeresgcesiesg ekug ege eege g ee 10 3 1 Top Level Menu Gtruciure 10 3 2 New Configuration Men 11 3 3 New Procedure Men 12 3 4 New Method Men 13 4 SYSTEM AND SOFTWARE SETUP 0 scsssssssssssssssssseseeeeees 14 4 1 System Configuration cece ee eeecee sees eee eeeecaeeeeee eee etecaaaeeeeeeeetnee 14 4 1 1 Show Configu
15. 7 at 15 22 60apstest on 16 Jan 07 at 15 53 60apstest on 16 Jan 07 at 14 57 60apstest on 16 Jan 07 at 14 50 G0apstest on 16 Jan 07 at 11 58 60apstest on 16 Jan 07 at 10 22 Preview Report Delete Results W410 Analysis Report Method 860ap tert Default Analyst Method Calibration nick8G0aps on 16 Jan 07 at 15 23 Performed on 16 0 1 2007 00 00 Performed by Uer Analysis Re suits Completed OK Page 1 ie Mion maple Heading SE SE tame Se Sne SE 15 23 11 Blank Dm 0 00 15 23 50 Calibration 10 00 115 10 00 15 24 15 Spot Check 10 00 H 9 91 Baias eeh napia 2 i CH Am 15 25 16 Sample Sample 2 Dm 0 00 0 00 15 25 55 Sample Sample 3 1n 11 42 11 52 Over range 15 26 28 Sample Sample 4 112 9 73 9 82 15 27 03 Sample le 5 102 8 85 8 93 E DEn SE im ES 15 28 06 Sample Sample 6 101 8 76 DR A 15 28 41 Sample Sample 7 106 9 03 9 11 If you click on Preview eeng See anus e 28 S1 En ES St ie aes aas H 15 30 21 Sample Sample 10 102 8 85 8 93 eport a small preview IS eneen 10 00 ie ss Dann deo a Maple 22 D iR su s 15 32 01 Sample Sample 12 102 8 85 8 93 pane WI appear WI IC you 15 32 34 Sample Sample 13 106 9 03 9 11 15 39 06 tamie e ie E CS 15 33 41 Sample Sample 15 102 8 85 8 93 can expand and or print as IL apet eneen ZS iS aos 15 34 47 Sample Sample 16 102 8 85 8 85 m H 15 35 24 Sample Sample 17 10 an 9 03 required an example IS 15 35 48 Sample Sample 18 aoa am va 15 36 28 ES Socie 19 im JI 10 97 over se 15 36 59 Sample Sam
16. Identity Name Originator Memo Calibration Name Select Always Calibrate Spot Check None First amp Last Every x Samples Concentration Sample Auto correction Number of Samples enter Fill in sample names in table Method Locked Operate Method Delete Method 410 91 008 Issue 2 22 October 2008 ECN 543 13 4 1 410 BlueNotesm Software System and Software Setup Before the Model 410 Flame Photometer is blanked and calibrated in preparation for sample analysis the 410 BlueNotes Software needs information about the instrument configuration being used and other parameters pertinent to the calibration and subsequent analysis to be performed The software allows storage of multiple System Configurations Calibration Procedures and Method Analyses for recall as required which can be setup by a Supervisor and then locked if required to prevent alteration System Configuration From the BlueNotes Start Page select System Configuration using the mouse pointer and click There is a choice Show an existing Configuration New create Configuration Delete Configuration Select an option using the mouse pointer and click 4 1 1 Show Configuration Show Configuration allows the user to review an existing configuration any changes made will prompt BlueNotes to note that the configuration details have been modified and ask if the changes should be saved BlueNotes 410 2 jJ The Configuration details
17. ading into the sample table along with the calculated concentration BlueNotes will then ask for the next sample to be aspirated and print to be pressed If the Analysis Method specified to not Always Calibrate then a previous calibration file can be used BlueNotes will prompt BlueNotes 410 Most recent calibration is test08 on 15 Oct 08 at 14 29 Perform new calibration Insert Sample If at anytime another sample requires analysis the method can be interrupted by clicking Insert Sample BlueNotes asks for a Sample Identifier and once the current sample has been analysed BlueNotes will request the new inserted sample for analysis before continuing from where it left off in the sample table 410 91 008 Issue 2 22 October 2008 ECN 543 24 410 BlueNotesm Software 5 2 2 With Model 860 Autosampler 5 3 Regardless of whether the Analysis Method selected specifies Always Calibrate BlueNotes will send the Model 860 Autosampler to aspirate a blank and ask the user to adjust the reading with the Model 410 Flame Photometer blank control if necessary and press print on the M410 If the Analysis Method specifies Always Calibrate the related calibration procedure will progress After the calibration is complete the Model 860 Autosampler will aspirate the first sample and then BlueNotes will wait until the Delay time has elapsed and then a steady signal obtained before entering it into the sample table along with the calculated sample c
18. after the sample introduction and poor precision may result Peak Selection Determines how a reading is taken Selecting Manual means the user decides when a stable signal has been achieved and then presses the print button on the Model 410 Flame Photometer Digital Interface to send the displayed reading immediately to the software Selecting Automatic means the software determines when the signal in the flame has stabilised after sample introduction In automatic mode the signal monitoring process is initiated when e the user presses the print button on the Digital Interface immediately the sample uptake tube attached to the Atomiser or Diluter is placed in the sample or e the Autosampler probe enters a sample or standard Peak Timeout time is used to give a time limit to the search for a stable reading when in Automatic Peak Selection mode Samples that give rise to unstable reading are highlighted in the analysis report 410 91 008 Issue 2 22 October 2008 ECN 543 15 410 BlueNotesm Software Instrument Warm Up Enter a Warm Up time This is the time required for the Model 410 Flame Photometer s flame assembly to stabilise after initial lighting In an analysis method the user may override the warm up period if the flame is already stable i e the flame has already been alight for gt 45 minutes Readings Select from a dropdown list the Units of concentration to be used to report sample concentr
19. ation after calibration conversion of recorded sample readings Configuration Locked Supervisors click on the Configuration Locked box to grey out user entered variables Alterations to the Configuration by regular Users will not be allowed Supervisors can unlock and make changes Save Click on Save and the Configuration will be saved under the name given in the Configuration Identity The Configuration can then be recalled from a list of saved Configurations and used in a Calibration Procedure 4 1 3 Delete Configuration If a configuration has been referred to later in a Calibration Procedure then clicking Delete Configuration will result in a dialogue box explaining that it cannot be deleted It is however possible to make changes to the configuration provided that it has not been locked by a Supervisor If the configuration selected for deletion has not been referenced in a Calibration Procedure then BlueNotes will ask to the user to confirm before deleting the configuration 4 1 4 Close Click Close to return to the BlueNotes Start Page 410 91 008 Issue 2 22 October 2008 ECN 543 16 410 BlueNotesm Software 4 2 Calibration Procedure From the 410 BlueNotes Start Page select Calibration Procedures There is a choice Show an existing Procedure New create Procedure Operate Procedure Delete Procedure Close to return to Start Page Select an option and click 4 2 1 Show Procedure Show Proced
20. ck the Save button to save the Analysis Method under the Name in Method Identity The Method can then be recalled to run an Analysis Method and analyse samples 4 3 3 Delete Method Clicking delete Method will prompt BlueNotes to ask the user to confirm before deleting the analysis method 4 3 4 Close Clicking close will return the user to the start page 410 91 008 Issue 2 22 October 2008 ECN 543 21 5 1 5 1 1 410 BlueNotesm Software Operation Operate Calibration Procedure To operate a procedure run a calibration click on Operate Procedure within the Calibration Procedure page of the software A blue dialog box will appear with prompts which guide the user through each step of the procedure Follow the instructions as they appear bearing in mind the following points NOTE don t forget set the 410 filter selector to the required analyte before adjusting the reading Systems with no Autosampler Blanking no Autosampler When asked to aspirate blank put the sample uptake tube of the Nebuliser or Model 805 Diluter into the blank and adjust the blank control on the Model 410 Flame Photometer to set the display to your required setting usually zero but does not have to be If the display is negative the software will not accept the reading when the print button is pressed In some cases it may be advisable to set the display to a slight positive such that any change in flame conditions that
21. d setup page the method will start with the Calibration Procedure The software will prompt the user to enter any sample identifiers names these may have already been entered when the analysis method was set up but this is another opportunity to edit e g update a daily method with new sample batch numbers Without Model 860 Autosampler Regardless of whether the Analysis Method selected specifies Always Calibrate BlueNotes will prompt to aspirate a blank Adjust the reading with the Model 410 Flame Photometer blank control if necessary and press print on the 410 If the Analysis Method specifies Always Calibrate the related calibration procedure will progress After the calibration is complete the user must evaluate the graph and make an assessment of the fit and decide if they wish to use it If the fit is too poor the Calibration Procedure can be run again If the calibration is acceptable e H the Configuration specified Manual Peak Selection then BlueNotes will prompt for the user to press print once a stable signal has been obtained aspirating a sample The displayed reading will immediately be entered into the sample table and the calculated sample concentration e f the Configuration specified Automatic Peak Selection then BlueNotes will prompt for the user to press print straight after the start of aspirating the sample BlueNotes will display and count down the tube Delay and then once a steady signal is obtained enter the re
22. down options list the details are shown to ensure the correct one is selected for the calibration procedure to be performed 410 91 008 Issue 2 22 October 2008 ECN 543 17 410 BlueNotesm Software Standard Details Choose the Analyte and select required number Format from the dropdown options Enter the maximum concentration standard Max Conc to be used results reported above this limit will be highlighted as over range Enter the number of standards you intend to use If working within the linear response range of the analyte a single point calibration is sufficient For multi point calibrations a minimum of five standards should be used it is unlikely that you would need to use more than seven Note if more that ten standards are used only ten data points will be displayed on the calibration graph although all point are used to generate the calibration equation Enter each standard value in the table in the order they are to be analysed and from the most concentrated first and descending in concentration If the standards are not entered in descending order an error message will prompt the user to change the order It is important that the concentration of the standards is added accurately into the table BlueNotes uses this information to generate the calibration equation and from that sample concentrations are calculated If a configuration was selected which includes a Model 860 Autosampler the vial numbers appear in the table
23. e instructions as they appear in the blue box until the procedure is complete 410 91 008 Issue 2 22 October 2008 ECN 543 22 5 1 2 5 1 3 410 BlueNotesm Software Systems with an Autosampler Blanking with an Autosampler The Model 860 Autosampler will automatically move to aspirate the blank and BlueNotes will instruct the user to adjust the blank control to set the display on the M410 to the required setting usually zero but does not have to be If the display is negative the software will not accept the reading when the print button is pressed in some cases it may be advisable to set the display to a slight positive such that any change in flame conditions that cause the blank reading to drop will still not send a negative reading to BlueNotes When the displayed reading achieves a stable level press the print button on the M410 Interface and the reading will be transferred to the calibration table in the software Top Standard with an Autosampler Immediately the Model 860 Autosampler will move to aspirate the top standard and the user is instructed to adjust the reading if necessary to the required level using the fine control and then press the print button be sure to wait for the signal to stabilise The blank and the fine controls on the M410 must now be left alone If it was necessary to change the course gain setting to achieve the desired reading for the standard the calibration process must be restarted as the b
24. how a method they have already locked then they will be able to unlock the method here 4 3 2 New Method To create an analysis method select New Method to reveal the following Sr BlueNotes 410 Supervisor MASTER File Supervisor Help deenen BlueNotes Analysis Method Method Identity Name Method1 Created Originator Supervisor Modified Memo Default analysis method Calibration Name 860410 x Multi point Na ppm Always Calibrate Spot Check None O FirstandLast O Evey Samples Concentration En H No of Samples 5 g Peak Selection Automatic iluter Identifier Diluter None 0 Method Locked Sample Sample Sample Sample Sample Method Identity Enter a Name indicate the Originator of the Analysis Method and any required comments in the comments Memo text box Note date and time created modified are shown but cannot be altered Calibration Select the Calibration Procedure you wish to use in association with the Analysis Method from the drop down menu 410 91 008 Issue 2 22 October 2008 ECN 543 19 410 BlueNotesm Software Always Calibrate If this box is checked the software will direct the user to re run the selected calibration procedure immediately prior to running an analysis method If the method does not specify Always Calibrate then the first time the method is run BlueNotes will inform that no calibration data is available and a calibrati
25. lank reading will not be relevant Subsequent standards with an Autosampler The Model 860 Autosampler will begin to aspirate the subsequent standards Once the blank and top standards have been set there is nothing further for the user to do the software will establish when a stable signal has been achieved enter the reading for the standard and continue on to aspirate the next one until all standards have been measured and their responses entered into the calibration Calibration Complete When the calibration is complete a dialog box will appear The calibration graph with it s correlation coefficient is displayed on the right of the screen If the fit to the line was not good enough the calibration will fail It is worth re running the calibration procedure to see if results are improved alternatively fresh solutions should be prepared See M410 Manual Troubleshooting 410 91 008 Issue 2 22 October 2008 ECN 543 23 5 2 5 2 1 410 BlueNotesm Software Operate Analysis Method To run an analysis click on Operate method within the Analysis Method page A blue dialog box will appear with prompts the user through each step In the bottom left hand corner of the display the Instrument Connection Status box confirms communication with the 410 and Model 860 Autosampler Follow the instructions as they appear bearing in mind the following points If always calibrate was selected in the Analysis Metho
26. mmunicate with your new 410 Interface Module from the START menu on the Task Bar Select All Programs Accessories Communications HyperTerminal New Connection and enter Name e g M410 Flame Coms1 Port Settings Bits per Second 9600 Data Bits 8 Parity None Stop Bits 1 Flow Control None and then Apply PCs operating within Windows Vista do not offer Windows HyperTerminal For those operators we have provided the 410 Digital Interface Terminal Facility which may be downloaded from the 410 BlueNotes Software disc This facility may also be used within other Windows Operating systems Display Spee nese Use Display to set the default parameters for the real time chart recorder displayed during operation of an Analysis Method There is a eo choice of coloured highlights for the result Waring sows Peck Timeout PS tables to flag sample results that gave either a calculated value above the Max Concentration defined in the Calibration Procedure or an unstable reading to indicate samples that might warrant re analysis Result over range E BlueNotes 410 Supervisor Options Users Connections Display Users Sherwood istofusers Within this page of the software User names may be added or deleted The requirement to enter a User name before an analysis method is run can be requested or disabled 410 91 008 Issue 2 22 October 2008 ECN 543 9 3 3 1 410 BlueNotesm Software Software Structure 410 BlueNotes
27. oducts Sherwood Scientific Ltd 1 The Paddocks Cherry Hinton Road Cambridge CB1 8DH England 410 91 008 Issue 2 22 October 2008 ECN 543 29
28. on must be performed The next time the method is run BlueNotes will provide the calibration file and date performed and allow the user to specify whether or not to accept this previous calibration or not Care should be exercised when accepting a previous calibration it is important that no instrument settings have changed especially if Spot Checks below are not selected as in this instance only a blank will be set by the user Spot Check and Sample Auto correction The 410 BlueNotes software allows standard spot checks to be performed during the analysis of a series of samples None First and Last or Every x samples Spot Checks are particularly useful in systems where many samples may be presented to the Model 410 Flame Photometer e g Autosampler systems where 40 samples may be presented to the flame photometer unattended Each time a Spot Check is called for the standard is presented to the Model 410 Flame Photometer and the response recorded Spot Checks are also useful in that they allow a previously saved calibration to be used if working in the linear range of the instrument for the analyte of interest then single point calibrations can be reused and adjusted with the use of the Spot Check in conjunction with the Auto Correct feature below If the Auto Correction facility is also selected then samples after a Spot Check measurement will be compared to the calibration equation adjusted by that mo
29. oncentration The routine will continue until all samples have been analysed Insert Sample BlueNotes display shows the progress of the Model 860 Autosampler if at anytime another sample requires analysis the method can be interrupted by clicking Insert Sample BlueNotes asks for a Sample Identifier and vial position Once the current sample has been analysed the Model 860 Autosampler will move to the new inserted sample vial position aspirate and analyse the sample before returning to continue from where it left off Reports When all the samples are complete a report is generated automatically Any sample results higher than the Max Concentration specified in the Calibration Procedure will be labelled as over range If Spot Checks were selected and Automatic Correction requested original and corrected results will be reported see example below 4170 Analysis Report Method Manteit Callbration manault on 23 Mar 07 at 03 51 Performed on 2303 2007 14 57 Performed by Usert tlt Results Completed OK Reading Sample Reading Result Corrected Mamo Type Identifier 14 57 33 Blank 001 0 00 0 00 00 Calibration 35 00 376 35 00 14 58 32 Spot Check 20 00 215 19 92 14 58 38 Sample Sample 1 340 31 56 31 68 14 58 41 Sample Sample 2 345 32 02 32 15 14 58 44 Spot Check 20 00 220 20 39 14 58 47 Sample Sample 3 450 41 80 41 00 Over range 14 58 51 Sample Sample 4 345 32 02 31 42 14 58 56 Spot Check 20 00 225 20 85 14 58 59 Sample
30. pecific exceptions that apply to the above statement are as follows 1 End user warranty time commences on the date of the delivery of product to end user premises Free of all charges statement applies only in areas recognised by Sherwood as being serviced either directly by its own personnel or indirectly through personnel of an authorised distributor Products purchased outside these areas requiring service during the warranty period will incur charges relative to the travel transit costs involved However products purchased in such areas will be serviced during the warranty period free of all charges providing they are returned carriage paid to either Sherwood or by pre arrangement to an authorised Sherwood distributor All maintenance other than operator maintenance as described in the instructions repairs or modifications have been made by Sherwood or Sherwood authorised personnel This product has where applicable been operated using Sherwood specified supplies and reagents Sherwood reserves the right to make any changes in the design or construction of future products of this type at any time without incurring any obligation to make any changes whatsoever to this particular product Reagents supplies consumables accessories and user maintenance items are not included in this warranty Repairs or replacement of any part failing due to abnormal conditions including the following are excluded from this warranty a
31. ple 20 103 8 94 8 94 shown ER Da eek SE in E 15 38 09 Sample Sample 21 10 8 76 8 84 E Satia Eer io Ei L 15 39 18 sampla Seepia 23 ioa sio sii 15 39 53 Sample Sample 24 093 8 05 8 13 15 40 28 Sampie Si om EE 7 95 15 41 33 Sapia maple 26 as aia aa 15 42 05 Sample Sample 27 093 8 05 8 13 15 42 38 Sample Si am EE soa 15 43 11 Sample Sample 29 103 EH 9 02 IER ES is rn L 15 44 16 Spot Check 10 00 H 9 91 15 44 44 Sample Sample 31 am 0 09 0 09 15 45 18 Sample Sample 32 am 0 09 0 09 15 46 24 Sample Sample 34 o0 0 09 0 09 SE EE anette a on 25 28 15 47 33 Spot Check 10 00 115 10 00 E Dh SL 36 A aos oos 15 48 38 Spot Check 10 00 HI 9 91 410 91 008 Issue 2 22 October 2008 ECN 543 27 410 BlueNotesm Software 7 Appendix A Warranty Sherwood Scientific Limited Product Warranty Statement Warranty Term 12 Months Sherwood Scientific Ltd Sherwood warrants subject to the conditions itemised within this document through either Sherwood personnel or personnel of its authorised distributors to repair or replace free of all charges including labour any part of this product which fails within the warranty time specified above appertaining to this particular product Such failure must have occurred because of a defect in material or workmanship and not have occurred as a result of operation of the product other than in accordance with procedures described in the instructions furnished with this product Conditions and s
32. ration ceccccesceceeeeeeeeeeccneeeeeeeeeeeecaaeeeeeeeeeeeensaeeeeeees 14 4 1 2 New CGonttouraton 14 4 1 3 Delete Contiouration 16 AR Melle 16 4 2 Calibration Procedure AA 17 4 2 1 SNOW Procecdure A 17 4 2 2 New Procedure A 17 4 2 3 Delete Method 18 4 3 Analysis Methods einan nannan a 19 4 3 1 Show Method 19 43 2 New Ete a nie 19 4 3 3 Delete Method 21 4 3 4 ee 21 5 OPERATION cimem e E a E E R E 22 5 1 Operate Calibration Procecdure 22 S11 Ree in Le 22 5 1 2 Systems with an Autosampler 23 5 1 3 Calibration CGomplete 23 5 2 Operate Analysis Method ccccccecccccceeeeeeeeeeeeeeccneeeeeeeeeeeeccaaeeeeeeeeeeeneaaaes 24 5 2 1 Without Model 860 Autosampler 24 5 2 2 With Model 860 Autosampler sesssssssssseressssssssrrnrrssssrrrrrnnsssssrrrrnnn 25 539 REPOS E 25 6 FILE ARCHIVES ege 26 6 1 Calibrati n reSults AAA 26 6 2 Analysis RESUS E E 27 7 APPENDIX A WARDANTY 28 410 91 008 Issue 2 22 October 2008 ECN 543 2 410 BlueNotesm Software 1 Introduction 1 1 Contacting Sherwood Scientific Ltd 1 1 1 If the 410 BlueNotes Software was supplied by a third party i e a Distributor in the UK or Overseas the first contact should be that Distributor for all issues of supply and operation Please note here the details of the Company where you purchased this unit COMPANY deg cesedueeevegevecnesecuexceeeseediteeedeeieeeeyy RRC ue 1 1 2 If the Software was obtained from Sherwood Scientific Ltd
33. st recent Spot Check reading 410 BlueNotes will report the instrument reading the concentration according to the initial calibration and the Auto Corrected result based on the most recent Spot Check result Hence any change in response due to drift in flame conditions with time may be kept to a minimum If Auto Correction is not selected the analysis of the Spot Check will be reported for the user to interpret any drift If using a Model 860 Autosampler the Spot Check is placed in the S standard position on the carousel Concentration If you select spot check enter the Concentration of the spot check standard Sample Auto correction Click the Sample Auto correction box if you require sample result correction in the final report relative to the most recent spot check reading No of Samples Set the number of samples to be analysed using the scroll box next to No of Samples The number of rows in the sample table will expand and if the Configuration specified includes an Autosampler the vial numbers will appear At this point the user may enter sample names and or numbers in the Identifier column max 16 characters 410 91 008 Issue 2 22 October 2008 ECN 543 20 410 BlueNotesm Software Method Locked Supervisors click on the Method Locked box to grey out user entered variables Alterations to the method by regular Users will not be allowed Supervisors can however unlock and make changes Save Cli
34. ure allows the user to review an existing Procedure any changes made will prompt BlueNotes to note that the Procedure details have been modified and ask if the changes should be saved If a supervisor has locked a procedure then a regular User will see the greyed out details and no changes can be made If a supervisor clicks to show a procedure they have already locked then they will be able to unlock the procedure here 4 2 2 New Procedure If New Procedure option is greyed out you need to login go to BlueNotes start page and select Supervisor Access from top level menu To create a calibration procedure select New Procedure to reveal the following gt BlueNotes 410 Supervisor MASTER file Supervisor Help ng BLUENOteS m Calibration Procedure Procedure Identity Name CalProct Created 21 03 2007 16 11 Originator Supervisor Modified 2i03 2007 16 411 Memo Default Calibration Procedure Configuration nickB60aps ei C Procedure Locked Standard Details Seq Concentration Vial Analyte Ba ppm 1 100 S Format nnnn e Max Conc 100 2 No of Standards 1 Se instrument Connection Status 1 m Procedure Identity Enter a Name indicate the Originator of the new procedure and record any required comments in the Memo text box Note date and time created modified are shown but cannot be altered Configuration Choose a Configuration from the drop
35. x and click on Log In Now when returning to Supervisor Access you may enter and save your own new supervisor password If at any time you wish to e View the operating manual Word or PDF format or e Watch the introductory presentation of the 410 BlueNotes Software or e Download the 410 Digital Terminal used to calibrate the digital output to the M410 display or retrieve the Digital Interface serial number Users of Windows Vista will need this facility users operating within Windows XP may use this facility or Windows Hyperterminal Click on the Help option in the top level menu on the 410 BlueNotes Start Page and select the required option from the drop down menu 410 91 008 Issue 2 22 October 2008 ECN 543 6 410 BlueNot sm Software 2 4 Connecting Peripheral Devices 2 4 1 Model 410 Flame Photometer Digital Interface Connect the host PC to the 410 Digital Interface using the serial connection cable provided The 9 pin D plug connects to a serial port on the PC If the PC does not have a spare serial port use a serial USB adapter 2 4 2 860 Autosampler The Model 860 Autosampler has a 9 way male D socket on the rear panel of the base compartment That socket needs to be connected to the host PC using the null modem cable provided part number 001 09 003 and a serial USB adapter part number 001 09 004 2 4 3 Model 805 Diluter There is no connection between the Model 805 Diluter and the software host PC
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