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Multiskan EX Thermo Scientific Multiskan EX User Manual

1. 90 Acknowledgement MessaglS coommocccccnnnnnnonnnnncccnn esent nnnr nantes nnne nets sees see en nn nennen tessera 36 DICTI M 52 pnl cce e TE 11 A ADDIICAtIOD sus racionis cara dank exo aue AER La eX ER A na CREE P RA CERERI a CAKE n Na EEE ERE Es 106 Autocalibration andre vasco cre dea eco a az 34 Autocalibration procedure terne ER ree aa gesdsavss E aara aean A Apa SER ea REESEE AGER iaka aai 42 B Baud Fate ccc cete A A A A o 28 52 SOLS CUUING ein A A A A A ORAR 29 Blanking on every platica A A a oi 52 Blanking possibilitiesS oooococncccccncnnnnonorororororo nnn nennen rre asa aan 51 C e mc 27 Calculation parameters Cubic spline program seen eect enter ener nennen nnns ntes nr RR inen nnns senseri enirn nnns 57 Cutoff progratn eicere nre nenne dE 55 Primary EIA progra Mii Reial A dada 53 Calibration CUIVE acicate add a dc a 14 Centronics interface seiersen aeaaea OS RAAEN EEA AEAEE RAAE EE AAEE AAEE RERE AEAN EAA 28 CITED 31 Certificate of Decontamination eese nennen nnn nnne nennen nnne nnne 105 Changing O A NA E 79 UA a a da 82 A sc aa ap a a a aea aa a a E a 81 paper rolls ia EMEN 85 Character TOMA diu A ii A A A A A eva pex Ex er 28 Check list MAMA ciao a iia eiii 74 O PEERS ETIN TIME 39
2. eeeeeeeeeeeeeee 88 How to pack for service nennen enne nennen nnne nnn nennen nnne nnn 88 Disposal of the instrument esses eene enne nnne enne nnne enn 88 Troubleshooting dint ERE HELD ardid 90 Troubleshooting guiden si araia a aeaa ar amaaan Ae eea AE a aana Eeo Tapa TAKAA GAEE aai anaa 90 Service request protocol ssr iii aara EA re iii E Ra True 92 Warnings and cautions iaaea Rt DRE eR RR ERR ne neon TE EE LEER PRAE R IER E Ees 92 Electrical eiui iii 92 Defects and abnormal stresses ssssssseeeeeeeeee nennen nnne nns 93 Technical Specifications leeeeeees esses esee eene enne nnne nnne nnne 94 General specification 94 Safety speciticatiONS usina ERR RE ER NER ERR x 95 In conformity with the requirements eeesseeeeeeeee enne 95 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 5 10 10 1 10 2 10 3 11 11 1 12 13 13 1 Appendix A Appendix B Appendix C Appendix D Table of Contents Ordering Information 5 e tarado iniciada died 96 L iSt OF ACCESS OMICS 1i eerta tree e ect aset ese ees List a d 96 Spares for maintenance sess nennen nnns nennt rra 96 Verification plates ergenin ia iia dic 96 Warranty Certificate ooo nn edis rsen coo Ee AA EnA RANNER RADERNA ERANA NARNIA den 97 Warranty liritStiOFis cocto ect te eb cay ee ec oos tr eec lar 97 O conebetectutedanctteraccetetiev
3. Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 52 Chapter 6 Operation 6 9 1 Measurement modes and parameters Table 6 3 Measurement modes Measurement mode 1 ABSORBANCE MODE Function The absorbances are measured at one wavelength also called an end point 2 DUAL WAVEL MODE 3 TWO POINT MODE The absorbances are measured using two wavelengths The results of the second measurement are subtracted from the results of the first measurement The absorbances of the plate are measured at two separate points in time The results of the first measurement are subtracted from the results of the second measurement 4 KINETIC MODE The rates of absorbance change are measured 5 MULTI WAVEL MODE The absorbances are measured using individual filter selection for each column of a microplate Only available with the Primary EIA program 6 COMPUTER CONTROL The instrument is controlled by an external computer Only available with the Primary EIA program Table 6 4 Measurement parameters Measurement parameter FILTER Function Enter the wavelength s of the filter s or the filter number s see 6 FILTER WHEEL in Table 6 8 on p 59 BLANKING POSSIBILITIES The following blanking possibilities are available in all available measurement modes 1 NO REAGENT BLANK 2 SINGLE WELL REAGENT BLANK e Enter the blank well 3 COLUMN MEAN BLANK e Enter the bla
4. 5 3 2 Installing the lamp Warning Only use the lamp approved by the supplier Cat no 2400620 Lamp quartz tungsten halogen lamp Osram 64607A 8V 50W 1 Unpack the lamp D Caution DO NOT touch the reflecting surface of the lamp or the bulb itself 2 Release the terminal socket wire from its transportation holder located inside the instrument 3 Hold the terminal socket vertically and the lamp so that the claw is pointing upwards Fit the lamp contacts to the terminal socket Fig 5 6 Press the lamp tightly to fit it properly Fig 5 7 Caution When fitting the lamp contacts be careful not to bend the contacts This might cause the bulb to detach from the lamp Warning If the instrument has been in use and you need to replace a burned lamp the lamp can be very hot Wait for the lamp to cool down before replacing it gt gt Fig 5 6 Fitting the lamp contacts 4 Place the lamp in its place so that the claw in the lamp fits in the groove Fig 5 7 Push the lamp all the way down Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 24 Chapter 5 Installation Fig 5 7 Lamp in its place 5 Visually check that there are no loose parts inside the instrument 6 Close the instrument cover and replace the two cover retaining screws Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 25 Chapter 5 Installation 5 3 3 Adjusting the plate carrier for different
5. 1 UNIT NO UNIT Select one of the units 3 UNIT IU I in this example LIN LIN Select one of the scaling possibilities LOG LIN in this example READY Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 73 Place the microplate onto the plate carrier Start the measurement of the first plate Parameters standard curve SENDING DATA concentrations are printed out CALCULATING Select whether the results are accepted or a new fit is 1 ACCEPT RESULTS required New fit in this example Select one of the LIN LIN scaling possibilities LIN LIN in this example PRESS PRINT SENDING DATA CALCULATING 1 ACCEPT RESULTS READY Remove the first microplate from the plate carrier and place the second microplate on it Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 Chapter 6 Operation 74 Start the measurement of the second plate Select whether the old blank can be used or a new blank on the plate is used instead Select whether the old curve can be used or a new curve is calculated instead Select whether the results are accepted or a new fit is required 1 USE OLD BLANK 1 USE OLD CURVE SENDING DATA CALCULATING 1 ACCEPT RESULTS READY Remove the microplate from the plate carrier Chapter 6 Operation The absor
6. Measurement circuit board cable D OTR ONA Fig 7 1 Opening the measurement assembly cover Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 78 Chapter 7 Maintenance 7 3 Cleaning the optical system 1 While the instrument is turned OFF remove the two 2 retaining screws Fig 5 2 item 7 of the measurement assembly cover Make sure that the plastic washers are kept with the screws Slide the cover slightly to the left and lift the cover 2 Clean the track Fig 7 1 item 4 inside the measurement assembly as mentioned above Caution DO NOT touch the measurement circuit board Fig 7 1 item 1 by hand 3 Clean the eight 8 focusing lenses at the end of the optical fiber bundle Fig 7 1 item 3 with a cloth dampened with water mild detergent or 9696 ethanol If 9696 ethanol is used wipe afterwards with a napless cloth dampened with water 4 The upper lenses are situated under the detector frame Fig 7 1 item 2 Remove the two detector frame fasteners with washers Fig 7 2 item 1 Lift the detector frame from the handle Fig 7 3 item 1 Clean the upper lenses Fig 7 3 item 2 in the same way as the focusing lenses Caution DO NOT use acetone to clean the plastic lenses focusing lenses or upper lenses Avoid harsh treatment 5 After cleaning the upper lenses lower the detector frame back to its position Caution Make sure that the Hall element sensor Fig 7 3 item 3 is po
7. 11 80 Removing the filt F SOFImg iii a T Ed Da d 81 Filtek wheel PIS s n d Re Tb o aaa 82 Individual filter showing the arrow 2 82 Changing the Dist a be ed 84 Changing theduses a t ded otc Rm erst mus 85 Measurement and calculation modes available in different program modules 16 KEV DOGIO KEV Seyi b sb a b o E a to Et 17 Installation CROCK AIST 2s a oder e E dre de rt a A Tor odes 18 DIL switch positions for baud rate selection DIL switch position for handshake smes in eenia pan e a seien 30 DIL switch positions for transmit receive selection oocccinnnnnininiccncnonanananananananane 31 Secondary DIL switch positions for Centronics type printer interface EE Data signals connected to the DB 25 connector NEC Computer control Commands uso te eese cet ee tdt be ees a Pr ada 34 Availability of computer control commands in different program modules 37 GJ T Te Moe CHECK MISE RM List of user defined assay programs m Measurement modes xar E eb aec e t Tee edo x ieee Measurement parameters inch T odi e d oe dai id Calculation parameters in Primary EIA program i Calculation parameters in Cutoff program oooooocociccccinnocccconono conan nc eene Calculation parameters in Cubic spline program sssssssssssssseenn ne General parameters od re tet ec aede th Default values of the general parameters Maintenance check list Example of the filters in the filter wheel positions 1 8 Troubleshooting gu
8. EX User Manual Rev 3 3 Cat no 1507300 56 Chapter 6 Operation Table 6 5 Calculation parameters in Primary EIA program Primary EIA program calculation parameter Operation 9 POINT TO POINT Enter the concentrations of the standards and the Multiskan EX calculates a piece wise linear standard curve with the aid of these standards The measured absorbances are converted to concentration units using this curve The measurement parameters calculation parameters and the concentrations obtained are sent via the interface If the piece wise linear standard curve is not monotonous the text NON MONOTONOUS curve will be printed and the results are incorrect Parameters Number of standards NR OF STDS Number of replicates NR OF REPL Standard concentrations STANDARD 1 etc Standard locations STANDARD 1 REPL 1 A1 etc Up to eight 8 standard solutions can be incorporated into the plate in up to three 3 replicates 1 STD ON EVERY PLATE Standard on every plate 1 STD ON EVERY PLATE or 2 RETAINED STD Retained standard 2 RETAINED STD the standard curve of the former plate is used can be selected If retained standard is selected 1 USE OLD LINE 2 NEW LINE NOW will be requested before reading the next plate 6 9 2 2 Cutoff program Table 6 6 Cutoff program calculation parameter Calculation parameters in Cutoff program Operation 1 NO CALCULATION The results are sent as absorbances via
9. controls POS CTRL 2 A1 There are two negative NO OF NEG CTRL 1 controls placed in wells D1 and E1 Location of the negative NEG CTRL 1 WELL A1 controls NEG CTRL 2 A1 Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 69 GREY AREA 0 000 Select either 1 gt or 2 gt With 1 the results above the cutoff are considered to be positive INTERPRETATION READY Start the measurement Place the microplate onto the plate carrier READY Remove the microplate from the plate carrier Print the raw measurement data READY The following results are printed in plate layout format e Raw data absorbances at 450 nm e Calculated results positive or negative Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 Chapter 6 Operation 70 Chapter 6 Operation 6 10 3 Example 3 Cubic spline program module e Module 3 Note The program module number depends on which modules are installed into your Multiskan EX instrument It may therefore differ from the program module number given in this example Absorbance measurement at 405 nm Blanking in well A1 a blank well only in the first plate Cubic spline curve fit with six 6 standards concentrations 7 30 50 85 130 and 200 in positions B1 C1 D1 E1 F1 and G1 e Same standard curve used for other plates Unit for standard concentrations IU I Two pla
10. fastener faces upwards Fig 7 2 i Caution DO NOT bend the measurement circuit board cable Fig 7 1 10 Slide the measurement assembly cover into its position Replace the two cover retaining screws The screw Fig 5 2 item 7 will connect the measurement assembly cover to the instrument ground to suppress electrical disturbances 11 After performing this decontamination procedure include a signed and dated Certificate of Decontamination Appendix B both inside the transport package and attached to the outside of the package Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 87 Chapter 7 Maintenance 7 9 Replacing the printer paper roll internal printer only If the paper of the internal printer is finished replace the printer paper roll as follows 1 Remove the old paper roll by lifting it The black paper roll shaft Fig 5 1 will come off with the roll 2 Make sure the edge of the paper in the new roll is straight no tears or creases Feed the paper evenly through the back slot as far as it goes without using any force Press the paper feed key 4 Insert the paper roll shaft inside the roll and put it back in its place 7 10 How to pack for service When you ship the instrument for service remember to e Inform about the use of hazardous materials e Decontaminate the instrument beforehand e Pack the instrument according to the enclosed packing instructions using the original pac
11. lt may therefore differ from the program module number given in this example Absorbance measurement at 450 nm Blank in well A1 Cutoff calculation with cutoff equation P N x 0 5 Grey area 0 02 Interpretation gt Check the printing the results are sent to the external printer 1 2 3 4 5 6 7 8 9 10 11 12 A Blank Un 4 B Pos 1 Un5 C Pos2 etc D Neg 1 E Neg 2 F Un 1 G Un 2 H Un 3 Fig 6 3 Plate layout in the example for Cutoff program module Switch the instrument on Wait one 1 minute Select the program module 2 CUTOFF READY Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 67 Chapter 6 Operation Select the meas measurement mode mode 1 ABSORBANCE MODE Select the meas param measurement parameters FILTER 340 Enter the wavelength of the filter or the filter position number FILTER 450 1 NO REAGENT BLANK Position of the blank 2 SINGLE WELL BLANK BLANK WELL A1 Blanking possibilities 1 BLANK ON EVERY PL READY Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 68 Chapter 6 Operation calc mode Select the calculation mode 4 CUTOFF and calculation parameters calc param CUTOFF EQUATION PRESS ENTER There are two positive NO OF POS CTRL 1 controls placed in wells B1 and C1 Location of the positive POS CTRL 1 WELL A1
12. A o 60 External printer Selectiva A A a ae lee 47 F Factor Calcula ii lala 53 ON RA 22 FECUDACK FOM Me tecet tt tata datar i n 106 FIDGMDU RG reece LE 12 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 99 Chapter 12 Index Filter NO 80 measurement DAME ons teaiin a oaie eiad a oaa a ae a ASEN AE RRA Ea ead aaia 51 performance testei aaiae inii a ies oec ses TES a a E 38 Filt r error A AA A A ki a kN Uu Eu re e TON a d x 87 Filter Spring xo ets emen en une anite 79 Filter wheel iri rerit a rando a e eai use Rege ee Er cra c F er acia 11 22 80 OS A erc redet ce ee Due d Etre cv dn e Dra ETC ade v t 23 number of filters and wavelengths ssesssseeesseeeeee nennen nnn nennen nnne annes 58 59 80 Filt r WINES ESIOE Ss ora ata o ea 22 Filters rne Em 79 Foc sirig lenses ao cs eret haee oa 12 76 III M A A A died T 27 90 Chad dictada 82 G General parameters ii a 47 57 default valles 3 niti a t i d f o gu Re ie E banda n d o a i E A d e 59 Ground coriniectiohu ciuitates intere acre da E C d ud 27 H Hall element SENSO hii ied iih been rera a vu Eta bep aee E ves A eed ded eua 78 84 Handshake Selecting eoe ts A orte x nene xe Eur 30 Handshake type cit HC 28 How to disinfect the instrument ccccccccccncnnnnnnnnnnnonononononononononcnonononononononononononononononono nono none nono nern
13. Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals Please include decontaminating solution used Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 109 Appendix C Multiskan EX Feedback Form Please send to Thermo Fisher Scientific Oy Fax 358 9 32910415 Thermo Scientific Multiskan EX Feedback Form Cat no Serial no PURCHASED BY PURCHASED FROM Company Organization Distributor Department Address Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Dr O Mr O Mrs O Ms O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions a O O O Instrument User manual O O O O Operational reliability O LH Oo O Design L1 O O o Ease of use a Oo a o Operational costs O O o O Customer support O Oo O O Further instrument system developments desired Further applications desired Where did you first learn about the product Would yo
14. CALCULATION The results are sent as absorbances via the interface 2 CUBIC SPLINE Cubic spline is a sigmodial curve used for example with hormones and tumor markers Parameters NO OF STDS 2 2 to 8 standards are allowed NO OF REPL 1 1 to 3 replicates are allowed STANDARD 1 0 000 Enter the concentration of standard 1 STANDARD 1 REPL 1 A1 Enter the location s of standard 1 STANDARD 2 0 000 Enter the concentration of standard 2 STANDARD 2 REPL 1 A1 Enter the location s of standard 2 STANDARD ON EVERY PLATE or RETAINED STANDARD 1 UNIT No unit U I IU I mIU I g l mg l mg dl ug l ug ml ng ml mmol l umol I nmol l 1 scale LIN LIN 2 scale LIN LOG 3 scale LOG LIN 4 scale LOG LOG After measurement and data transfer the program requests if the results are accepted or if a new fit is required 1 ACCEPT RESULTS 2 NEW FIT If you accept the results the instrument goes to the READY state If a new fit is required you have to select new scale parameters and press the PRINT key The new results will be interfaced In case retained standard is used 1 USE OLD CURVE 2 NEW CURVE NOW is requested to be selected 6 9 3 General parameters You can change the general instrument parameters using the param key See also Section 6 8 Table 6 8 General parameters Parameter Function 1 RS FORMAT 1 PRINTER FORMAT header information and absorbances are sent via the interface 2 COMPUTER FO
15. CheCckSUlm CIFOR oes cic roo aa TO 87 CROPPER seas ciciivaa Lm 22 Chopper speed Eric 87 Chopper Wheel liada DID DENTIS II RIDENS 11 12 Cleaning iniStEu mel E 686 scat tesecuet A A A endis VERE dud UER EROR 76 Bod c T 58 59 Column SubstractiQR iiia inr eio cette cbse tee Cu ce ocu su nee Duca ce day qu nda cR n cadat nado CR ERR dada Uno Lak Ua deen 54 Complaim Ordn asse eenno ee A sea adi Red deeds pe E EEEE RAEE 88 Computer Cem A T 47 Computer CONTON essiensa eee ocv sa a la 34 51 in different program modules occccccnnnccccnnononnncnnnnnnncnnn nono nana eene enn nn en arrancan rra 37 Connecting to a computer or a printer nennen nennen nnns nenne nn nnns innen nnn 28 Connector for built in printer eesseesseseseeeee esent 22 Connector for Centronics interface optional RS 232C interface or optional IEEE 488 interface 22 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 98 Chapter 12 Index Consumables warranty MIOS ir 93 Contact Information e ete iD t ede dab cle cca denn vec aq cs beetles dd nda ita dd case 107 Contamination PEI e ne ose sce DAD Loa ee v fet tUm Ee EM ete 42 Control panel PRO 17 Clica a 75 Cubic Spline pee 57 Cubic spline program module calculation Parameters gis scun ien irte ptg ceto A 57 CAMPO ina e e eH Ole eo elite eo meii o Pn reti teres en 69 CU pe 56 Cutoff program module calc lation paramietets onsi siie iiit pec eerte ter ied bre tee pa t
16. Shows any visible damage 2 Fails to perform the intended functions 3 Has been subjected to prolonged storage under unfavorable conditions 4 Has been subjected to severe transport stresses Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 93 Chapter 9 Technical Specifications 9 Technical Specifications 9 1 General specifications General specifications Overall dimensions 140 mm H x 420 mm W x 320 mm D 5 5 H x 16 5 W x 12 6 D Weight Basic unit 11 kg 24 Ibs Mains power supply 100 240 Vac 50 60 Hz Power consumption 170 VA max Heat dissipation 580 BTU Fuse requirements 2x3 5A slow blow type UL 198G Time Delay 5 x 20 mm Operating conditions 10 C 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only Transportation conditions 40 C 70 C packed in transport packaging Storage conditions 25 C 50 C packed in transport packaging Microplates 96 well plate Optical system Quartz tungsten halogen lamp Osram 64607 8V 50W chopper wheel aperture and condenser lens semitransparent mirror interference filter in filter wheel fiber bundle focusing lenses upper lenses and detectors Wavelength range 400 750 nm Filters 8 position filter wheel Standard filters 405 nm 450 and 620 nm Half ban
17. WELL A1 Blanking possibilities 1 BLANK ON EVERY PL READY Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 63 calc mode Select the calculation mode and calculation parameters 9 POINT TO POINT calc param Number of standards NR OF STDS Number of replicates NR OF REPL Concentration of the standard STANDARD 1 0 000 Location of the standard STD 1 REPL 1 A1 replicates STD 1 REPL 2 1 i STANDARD 2 0 000 STD 2 REPL 1 A1 Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 Chapter 6 Operation 64 The standard curve is calculated for every plate Save the program Thermo Scientific Multiskan EX User Manual STD 2 REPL 2 A1 STANDARD 3 0 000 STD 3 REPL 1 A1 STD 3 REPL 2 A1 1 STD ON EVERY PLATE READY PROGRAM NUMBER 1 SAVING DATA READY Continued Rev 3 3 Cat no 1507300 Chapter 6 Operation 65 Start the measurement Print the raw measurement data Thermo Scientific Multiskan EX User Manual Place the microplate onto the plate carrier READY Remove the microplate from the plate carrier READY Rev 3 3 Cat no 1507300 Chapter 6 Operation 66 Chapter 6 Operation 6 10 2 Example 2 Cutoff calculation using equation Module 2 Note The program module number depends on which modules are installed into your Multiskan EX instrument
18. an individual filter is faulty Check the filter Contact authorized technical service NO LIGHT The lamp has burnt out The optical light path is obstructed Change the lamp Check also the previous measurement results in case the lamp has burned in the middle of the measurement Check the instrument and filters Change the filter if damaged CHOPPER SPEED ERROR Chopper motor control faulty Contact authorized technical service NO CENTRONICS PRINTER The parallel Centronics type printer is not connected The printer has no paper Connect the Centronics printer Check the printer settings Check the option switch no 2 Section 5 4 5 Add paper to the Centronics printer PRINTER ERROR The Centronics printer is not switched on Switch the Centronics printer on SAVING ERROR Saving the program is not successful Contact authorized technical service CHECKSUM ERROR Loading the program is not successful Contact authorized technical service OUT OF RANGE The value entered is out of available value range Enter a value that is within the instrument s range NOT AVAILABLE The selection is not available Enter another selection EQUATION ERROR 1 5 There are errors in the cutoff equation Make corrections to the cutoff equation EQUATION ERROR 1 The first character in the equation is Should be EQUATION ERR
19. carrier Check that the filter and the plate are correct and the plate is placed properly on the plate carrier Fig 5 2 item 9 with the A1 well in the upper left hand corner Fig 6 1 It is recommended that the assay includes internal quality controls ers MEASURING The instrument performs the measurement READY After the measurement the results are printed on the internal printer or sent via the interface and recorded on the external printer or the computer according to the selection see Section 6 8 1 Check from the printout that the used general and measurement parameters were Remove the microplate from the plate carrier as intended f 76 8 H gP 0 N poper feed out obs sove recall porom 0 oe 4 5E 6 gi pi oe ry e 14 2 B 3 C C enter start stop meos mode cok mode y Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 107 Appendix A Multiskan EX Quick Reference Guide 1 Selectthe program module See Table 4 1 1 PRIMARY ElA 2 CUTOFF 3 CUBIC SPLINE READY 2 Selectthe measurement mode and measurement parameters See Table 6 3 on p 53 and Table 6 4 on p 53 Select the measurement parameters Select the measurement mode READY 3 Select the calculation mode and calculation parameters See Table 6 5 on p 55 Table 6 6 on p 57 and Table 6 7 on p 59 Select the calculation para
20. computer control mode the Multiskan EX responds to the following commands Table 5 7 Computer control commands Command Function R Starts the computer control F1 F8 Filter selection FMnn n An individual filter is selected for each column n filter number numbers from 1 to 8 can be selected EO E1 Selection of a continuous plate movement Selection of a stepping plate movement C The current program module number is sent Cn Selection of program module n 1 3 where 1 Primary EIA module 2 Cutoff module 3 Cubic spline module Note After selecting a new program module autocalibration takes place 25 s A new R command has to be entered now in order to return to the computer control Air blank Reagent blank on column defined by command S Measuring the whole plate and sending the results via the interface in microplate format PM Measuring the whole plate using an individual filter for each column and sending the results via the interface in microplate format Pn Measuring the whole plate n times n 1 99 and sending the results via the interface in microplate format Measuring n columns commencing from the starting column n 1 12 Sj Determining the relative starting column j 1 12 Thermo Scientific Multiskan EX User Manual Continued Rev 3 3 Cat no 1507300 34 Chapter 5 Installa
21. cutoff equations Arrow down to scroll forwards in the list of modes or parameters y and to change the clock display Parenthesis close bracket used in cutoff equations Rev 3 3 Cat no 1507300 17 Chapter 5 Installation 5 Installation 5 1 Installation check list A gt gt gt B Table 5 1 Installation check list Tick Item a Caution a a Warning a n O Caution Caution a Caution DD O O Unpack the Multiskan EX instrument carefully with the arrows on the transport package pointing upwards Refer to the enclosed packing instructions Keep the original packaging and packing material for future transportation Use of alternative packaging materials may invalidate the warranty To lift the instrument put your fingers under the bottom on both sides and lift it with your back straight taking proper precautions to avoid injury The net weight of the instrument is 11 kg 24 Ibs Check the delivery for completeness Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative Check the transport package the instrument and the accessories for damage during transport Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument Refer to Section 5 2 Place the instrument on a normal laboratory bench taking into account both the environmental and tec
22. die 57 C bic spline prOQgralm 5 5 2 mhi e daria eed EE EE ERR 59 General Parameters iii a daa a aia 59 Examples on how to use the Multiskan EX sess 62 Example 1 Primary EIA program module sess 62 Example 2 Cutoff calculation using equatiON oooccconoccncnanonnnnnnnnnnnncnannnnnronan acarician 67 Example 3 Cubic spline program module eeeeeeeneeeennennnn 71 Maintenance iicistevitscd sectestenis d sescudetccaninshaelveauecdseexewessaentaad dceneeesteeaunacaeeateesaseletateeceeaiease 76 Regular and preventive maintenance sess 76 Servico CONTACTS ss che Hoi ne td eed ada rie dE e eda edi eds 76 Maintenance check list enne enne nennen nennen 76 Geperal mc 77 immediate iii er OR a OV ED SORGE OP RU nr ERRARE RERO EUER 77 Filter Maintenance Rito e Ie tia 77 Routine cleaning of the instrument essen enne nnne nnne 78 Cleaning the optical system esssssessssseseeeee eene enne nn nnne nnns 79 Changing individual filters in the filter wheel eeeeeseeeeenennennn 81 Changing the lamp iii dd illa 83 Changing the fUuses iii 85 Disposal of materials retreat eer dne nena nba naar ad 85 Decontamination procedure sess enne nnn nnne nennen 86 How to decontaminate the instrument sess 86 Replacing the printer paper roll internal printer only
23. important for accurate measurement It is obvious that bubbles or foam on the surface will scatter light in all directions and make photometry inaccurate Experience shows that bubbles or foam are seldom a practical problem if proper procedures in pipetting are followed e g the use of reverse technique in pipetting solutions which tend to foam easily Diluted aqueous solutions generally produce an even flat surface which functions as a second window of the cuvette in the normal way However in many cases considerable concentrations of protein are present or detergents are used to avoid turbidity In these cases the surface is concave and Fig 4 2 demonstrates the consequence Fig 42 Flat and concave liquid surfaces The height of the column of solution will be reduced in the center of the cuvette where almost all light passes Although the volume remains the same Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 13 Chapter 4 Functional Description the effective light path is shortened and the apparent absorbance of a given amount of light absorbing substance in the cuvette is reduced Practice shows that in most cases the absorbance is reduced approximately by a factor of 0 9 If a calibration curve is prepared it will have a lesser slope than the ideal curve This effect is shown in Fig 4 3 curves 1 ideal and 2 actual A E 3i B C 1 Ideal curve 2 Actual curve due to shorter height of column o
24. including installation and operating instructions Read the manual in its entirety prior to operating the instrument This User Manual has been designed to give you the information you need to e Review safety precautions e Install the Multiskan EX e Carry out photometric measurement and calculation procedures e Perform basic maintenance procedures e Troubleshoot the instrument performance e Maintain the instrument This User Manual also describes features and specifications of the Multiskan EX hardware and on board software in Chapter 9 Technical Specifications Chapter 6 explains the operating procedures The user should be familiar with the contents of Chapter 7 on maintenance For warranty and ordering information refer to Chapters 10 Ordering Information and 11 Warranty Certificate Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 9 Chapter 3 Introduction to the Multiskan EX 3 Introduction to the Multiskan EX The Multiskan EX is a standalone photometric microplate absorbance reader including internal software The Multiskan EX can also be controlled by a computer Your local Thermo Fisher Scientific representative can arrange instrument training at the commissioning of the instrument for extra charge if required 3 1 Intended use The Multiskan EX is a microplate photometer for measuring absorbance from suitable microplates and strips in 96 well plate format mentioned in this manual that meet th
25. of 1 5 is set the segments are as follows Segment Measured absorbance value in range Printout 1 A lt 0 150 0 2 0 150 lt A lt 0 300 1 3 0 300 lt A lt 0 450 2 4 0 450 lt A lt 0 600 3 5 0 600 lt A lt 0 750 4 6 0 750 lt A lt 0 900 5 7 0 900 lt A lt 1 050 6 8 1 050 lt A lt 1 200 7 9 1 200 lt A lt 1 350 8 10 1 350 lt A lt 1 500 9 The measured absorbance value is compared to a cutoff value calculated with the aid of a cutoff equation using measured absorbances of positive or negative controls Enter the equation with the aid of X h P L and N keys Example P N x 0 5 Enter the number of positive controls Enter the positions of positive negative or low positive controls See pos control above See pos control above Enter the absorbance range where the results obtained are doubtful results inside the gray area are presented with O Interpretation of the results is chosen 1 gt the results above the cutoff value are presented with the results below the cutoff are presented with 2 2 the results above the cutoff value are presented with the results below it are presented with Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 58 6 9 2 3 Cubic spline program Chapter 6 Operation Table 6 7 Calculation parameters in Cubic spline program Cubic spline program calculation parameter Operation 1 NO
26. see Section 10 3 See Section 7 4 on replacing filters Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 77 Chapter 7 Maintenance 7 2 Routine cleaning of the instrument Clean the instrument regularly as stated below It is recommended that the case of the instrument be cleaned periodically to maintain its good appearance It is particularly essential that the plate carrier be clean and dry to prevent jamming A soft cloth dampened in warm mild detergent solution will suffice the cleaning agent as recommended by the manufacturer DO NOT expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Caution Painted surfaces can be cleaned with most laboratory detergents Dilute 1 Turn the power OFF and unplug the instrument 2 Use disposable gloves 3 Clean the instrument outside the track Fig 7 1 item 4 and the plate carrier Fig 7 1 item 5 with a cloth dampened with water or mild detergent 4 If any surfaces have been contaminated with biohazardous material a mild AN sterilizing solution should be used Caution DO NOT use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the finish ES Li LI 1 1 I I L LI LI 1 1 LI 1 l I Measurement circuit board E Detector frame Focusing lenses Track rails Plate carrier
27. the instrument display Perform action Indicates the actions you should take You find a table showing all programming parameters and a detailed description of the different measurements and calculations in Section 6 9 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 39 Chapter 6 Operation 6 2 Making a program Select the program module See Table 4 1 1 PRIMARY EIA 2 CUTOFF 3 CUBIC SPLINE 2 Select the measurement mode and measurement parameters READY Select the measurement parameters Select the measurement mode READY Select the calculation parameters Select the calculation mode READY 4 You have now made a program Save it to the instrument memory on READY o Note It is recommended to keep a record of stored programs You can use 1 Table 6 2 for this purpose Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 40 Table 6 2 List of user defined assay programs Chapter 6 Operation Program no Program module Name of assay and comments PHOTOCOPIABLE Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 41 Chapter 6 Operation 6 3 Measuring Use the power switch at the rear of the instrument Fig 5 9 item 1 Switch the instrument on To prolong the
28. used can be selected If retained standard is selected 1 USE OLD LINE 2 NEW LINE NOW will be requested before reading the next plate You can define the standard line by entering the slope and the intercept by using the known standard line The Multiskan EX calculates the concentrations using the following equation absorbance A x concentration B where A slope as in the linear standard mode B intercept concentration Error The measurement parameters calculation parameters and the concentrations obtained are sent via the interface Parameters LINE SLOPE INTERCEPT The measurement absorbance values are compared to a limit value defined by the user If the result is lower than the set limit a negative sign is sent otherwise a positive sign The measurement parameters calculation parameters and the results in a matrix are sent via the interface Parameters Limit value LIMIT 0 000 2 000 Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 55 Table 6 5 Chapter 6 Operation Calculation parameters in Primary ElA program Primary ElA program calculation parameter Operation 6 DOUBLE LIMIT The measured absorbance values are compared to two 2 limit values defined by the user If the result is lower than the lower limit a negative sign is sent If the result is between the two limit values 0 is sent If the result is higher than the upper lim
29. 2 2 5 2 3 5 2 4 5 2 5 5 2 6 5 3 5 3 1 5 3 2 5 3 3 5 3 4 5 4 5 4 1 5 4 2 5 4 3 5 4 4 5 4 5 5 4 6 5 4 7 5 5 5 5 1 5 5 2 5 5 3 5 6 6 6 1 6 2 6 3 6 4 Safety Symbols and MarkingS ooocccononoccconococcconononncnnnnonnnnnnnnannnnonn cnn nrnnnnnnnnnnnnnnnnn 8 About the User Manual 5 erii nce te ecacte nannte roni nna reda sa cria idas uta 9 Introduction to the Multiskan EX ooonnoononcccn nnncconononcncnnncnnnnnnnnnnnncnnnnnnnnnannnnnnnnnnnnnnns 10 Itende USE C iaa 10 Principle of operation deca tnc Tete tro E Dre a a 10 Advantages of using Multiskan EX ooonooccccnnoccnncnanonnnonannononanononorana nn nn crann nn nennen nns 10 Functional DescriptiON oconmnonccccononncnnonoccncnoncnnnannnnnnnnnnnnnnnnnnnnn cren nnnrnnnnnnnn nr nennen nnn 11 Measurement principles aa aE rre 11 Vertical measurement principle ooooccnnnnnnononononenananoncncnnn cnn nn nn 12 Surface of the liquid in a CUVet8 ooccconoccccnanoncncnanonnnnnnanonnncnnna nn nnnnnonnnnnnnr a nernnnr arenas 13 Program modules AA cedet dese 15 KeybDOard ia EE 17 Installati n ESSE M 18 Installation check list 4 iaa 18 What to do upon delivery essssssssessessesee eene nnne enne nnne nnne 19 How to Unpack asi 19 Checking delivery for completeness sees ene 19 Checking for damage during transport esseseseeeeeeeenneneen n
30. 9 3291 0415 www thermo com Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 2 Table of Contents Table of Contents O o N Oo C Bb Y N 0X 0 N 0 13 Appendix A Appendix B Appendix C Appendix D Safety Symbols and Markings eeseeeeeeseeseee eese eene nnne 8 About the User Manual recenter entrer nere rone rera ii reia 9 Introduction to the Multiskan EX eene nenne 10 Functional Description serere cance cence eenassapeuccecceexeusstaelenesaereveess 11 IMS tall ati Om iere niece eee N 18 Operation sacris a enun iuit iioi 39 ME EET DEED 76 Troubleshooting 5 4 Iter odian 90 Technical Specifications trei eren cera pocta RA 94 Ordering Information ima id Le Su CER Pen 96 Warranty Certificate ecrit RR 97 A OT 98 Glossary and Abbreviations ccccccssssceesseneeeeessaeeeesseaeeeesseaeeeenseeeeseesseeeeeesenaes 105 Multiskan EX Quick Reference Guide ooococooncccconncccncnconcncnnnncnnnnnncnannnnnnnnnnnnncnns 107 Certificate of Decontamination eeeseseesseeeeenenen nennen nnne nnns 109 Multiskan EX Feedback Form esee nennen 110 Addresses LC 111 Notes MT MM 112 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 3 Table of Contents Table of Contents 3 1 3 2 3 3 4 1 4 1 1 4 1 2 4 2 4 3 5 1 5 2 5 2 1 5
31. AT EN 42 Stop key o ABRIR IER enu A ned a 34 T Tooxtaucbh ligli eo hoc a TO ed eeu A E a tna Tee ears oes 87 Total time eem ee RE etuer etii et e ERU E Qn 52 RACK c Em 76 Cecontamimation iui Eee ete t EAL Re ce bend ese Eua ce eu edens 83 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 103 Chapter 12 Index MAA OS 21 Transmit receive pin configuration ooonncccnnnnoccccnnononcnnnnnoccnn nano crono rre 28 31 ir nsport damage aar irain A A A A ee cetera detur t 20 Transport package a x nuege etie I eo La RED ERE Roa 18 19 20 WTiroubleshOOLLiQ c iare 8 tac a ood rise dete oec eis eei c ee ie tense ered eee ne t 86 Two point measurement mode c ccccccnnnnnnnoncnininonnnanenn nr rn 51 U Unpaid Eug 19 Upper II MC nds 78 V Verification plates DP 92 Vertical Photometry Ac Ad fei ei ettet lee nl E tele 11 W Warm Up tE A gs lng whee ca renee dae beh Sass 38 MM ai 18 19 93 Wavelength Select visir ii laa a A a AAA A AA aea dee Eo 58 59 Well diia dd A A A ee T E A 18 19 20 90 Working a RE e REM ERE 20 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 104 Chapter 13 Glossary and Abbreviations 13 Glossary and Abbreviations Absorbance Negative logarithm of one minus absorptance as measured on a uniform sample Blank s Blanks in assays Reagent blank reagent blank without any sample The reagent blank wel
32. OR 2 The number of parentheses is uneven Should be even Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 91 Chapter 8 Troubleshooting Error Cause Action EQUATION ERROR 3 The first symbol in the cutoff Should be or operand equation is not nor an operand EQUATION ERROR 4 The operator is not followed Make corrections by C or an operand EQUATION ERROR 5 There are two operands after Make corrections each other in the equation If you were able to correct the error without having to turn off the instrument you may continue instrument operation by pressing start 8 2 Service request protocol If the Multiskan EX requires service contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s service department DO NOT under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your Thermo Fisher Scientific representative or distributor will take care of sending a complaint form Complaint order to Thermo Fisher Scientific s service department The Complaint order contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill in and forward the Complaint order to Thermo Fisher Scientifi
33. RD 1 REPL 1 A1 L 1STD ON EVERY PLATE 2 RETAINED STD L 1USE OLD LINE 2 NEW LINE NOW 1 NO CALCULATION 2 DOUBLE LIMIT L LOW LIMIT L HIGH LIMIT 3 RANGE CALCULATION L RANGE 4 CUT OFF L CUT OFF EQUATION L NO OF POS CONTROL L POS CTRL WELL A1 L NO OF NEG CONTROL L NEG CTRL WELL A1 L NO OF LOW CONTROL L LOW CTRL WELL A1 L GREY AREA L INTERPRETATION L1i gt 2 gt 1 NO CALCULATION 2 CUBIC SPLINE L NO OF STDS L NO OF REPL L STANDARD 1 0 000 L STANDARD 1 REPL 1 A1 L STANDARD 2 0 000 L STANDARD 2 REPL 1 A1 L STD ON EVERY PLATE RETAINED STANDARD L 1 USE OLD CURVE 2 NEW CURVE NOW L 1 UNIT NO UNIT 2UNIT U I 3 UNIT IU I 4UNIT mlU 5 UNIT g l 6 UNIT mg l 7 UNIT mg dl 8 UNIT ug l 9 UNIT ug ml 10 UNIT ng ml 11 UNIT mmol l 12 UNIT umol l 13 UNIT nmol l l1 LIN LIN 2 LIN LOG 3 LOG LIN 4 LOG LOG L 1 ACCEPT RESULTS 2 NEW FIT 1 RS FORMAT L 1 PRINTER FORMAT 2 COMPUTER FORMAT 2 PLATE MOVEMENT L 1 CONTINUOUS MODE 2 STEP MODE 3 PROGRAM PRINTING 4 PRINTER ON OFF L 1PRINTER OFF 2 PRINTER ON 5 PLATE ANALYSIS L 1 ANALYSIS OFF 2 ANALYSIS ON 6 FILTER WHEEL L NO OF FILTERS 1 L FILTER 1 WAVEL 405 7 CLOCK SET 8 PRINTOUT SPACING L 1 NO SPACING 2 PRINTOUT SPACING 9 MIXING L 1 MIXING OFF L 2 MIXING ON L 1 MIXING FAST 2 MIXING MEDIUM 3 MIXING SLOW L MIXING TIME 10 MODULE LISTING
34. RMAT only absorbances are sent via the interface in plate format 2 PLATE MOVEMENT You can select the speed at which the plate is moved 1 CONTINUOUS 5 s 2 STEP MODE 25 s 3 PROGRAM PRINTING Select this parameter to print out with the printer the programs stored in the memory of the instrument 4 PRINTER ON OFF On Off selection of the printer When you select Off the SELECTION results are automatically sent via the interface When the instrument is switched on the latest selection will be valid Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 59 Chapter 6 Operation Table 6 8 General parameters Parameter Function 5 PLATE ANALYSIS 6 FILTER WHEEL After the results have been presented in microplate format the instrument sends the mean value minimum value maximum value delta value standard deviation value delta percentage and the coefficient of variation percentage for every column and row as well as for the whole plate via the interface You can select whether to perform the analysis or not 1 ANALYSIS OFF 2 ANALYSIS ON not available in the Cubic spline program The number of filters and filter wavelengths can be entered See Section 6 8 4 NR OF FILTERS 1 The number of filters 1 8 can be entered or changed with the arrow and number keys FILTER 1 WAVEL 405 Filter wavelengths in nm can be entered or changed with the arro
35. RS 232C interface or via a parallel Centronics type printer interface 3 3 Advantages of using Multiskan EX The Multiskan EX provides several advantages relating mainly to the principle of operation in that it has e Aproven optical system e A flexible internal software e Software for PC control e Exceptional reliability e An extended 3 year warranty Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 10 Chapter 4 Functional Description 4 Functional Description 4 1 Measurement principle Photometric measurement The Multiskan EX utilizes the original Thermo Fisher Scientific Oy concept of vertical photometry in which the light beam passes through the whole sample In vertical photometry the absorption of light is proportional to the amount of light absorbing substance in the well The advantages of the vertical light path measurement are e Inaccurate pipetting of non absorbing liquids does not affect the measured absorbance values e Evaporation of non absorbing liquids during the reaction does not affect the measured absorbance values e A certain degree of non homogeneity in the solution for example as a result of layering in turbidity measurements does not affect the results The optical system comprises the following components Fig 4 1 Quartz halogen lamp Chopper wheel Aperture and condenser lens Semitransparent mirror Aperture Interference filter filter wheel Optical
36. SR handshake The handshake type used is set by the manufacturer You can select the desired alternative through a DIL switch at the rear of the instrument see Section 5 4 3 The transmit receive pin configuration is pin 2 transmits and pin 3 receives or vice versa You can select the desired configuration through the DIL switches at the rear of the instrument see Section 5 4 4 The following configuration is set by the manufacturer pin 2 transmits and pin 3 receives The factory set DIL switch positions are given in Fig 5 10 The position 4 in the secondary interface DIL switches Fig 5 9 item 7 is reserved for service use and it should always be in down position The positions 5 to 8 in the secondary interface DIL switches are not in use and it is recommended that they are in down position Em m mm E LEE Em mm mm mE m m Lm LE 87654321 87654321 Fig 5 10 Factory set DIL switch positions viewed from the rear as shown in Fig 5 9 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 28 Chapter 5 Installation 5 4 2 Baud rate selection You can select the baud rate i e the speed at which data is changed between the Multiskan EX and the computer through the DIL switches 1 2 and 3 Fig 5 11 a um a EA A UI IC A EP Saw aU 87654321 876 54 Fig 5 11 DIL switch positions 1 2 and 3 for baud rate selection Table 5 2 DIL switch positions for baud rate selecti
37. SaViIND Hz THO EAEE E EN E ESTESA LI 87 SCANPRI2 printer circuit board eano enai aeaa nnne nennen nenne aiaa eaa daaa adaa a assa nnne nas 22 Secondary interface DIL switches ooonnccccnnnocccnnnononncnnanonncnnn no nc n nro nr nano rra rr rr 27 Selectig handsbake A A a Ta O A 30 Selecting Dad rte A A A A A al 29 Centronics Interface ee cierre Dl di 31 computer Modern ae aa a Dada iio 34 keyboard Usd A AA A 34 MINO rr dic 49 58 59 n mber ot filters n A A edes Fre AAA AAA a 58 59 transmit receive pin CconfiguratiON occcnnncccnnnnocncnnononnnnnnn nn cnn naar cnc enne rre rr 31 MIA a ainda edt ai iaa 58 59 Semitranslucent mmilfOF s iii lt A A AO A AA de Ad 12 Semiiranspare NEMT OL eia a a a E a a tii 11 Serial RS 232C DIES Witt E tr teo Fr cep ote E D RE EEA AAEE RR REO EER 27 SORVICS ai da 75 88 packing or A a a reas eiaed ac te 85 request protocol n AEA E A a ee 88 Setting HANA MN acid 58 59 SHAKIRA da A EA AA iaa da 49 58 59 Site of operatiOh i 3 octets rice et eee temer eee Per doe vete Poe a ee Pe T vk E Ce ra Ve EP D e T 20 A E A desc 92 Specifications dt a DRE eR COD 90 JO AA a 90 MY A A A Ad 91 SM OG WERE A es Sees eae Sete 90 Speed of operation etit teet ertet ge eodera E LASNE A EERE EEA EREE EREE EN EERE 90 Standard lei de 53 Standardization and calibration procedures sess nnn nnns 15 start k6y nodules Aa eat IA 42 rl EE E A T A OE E cues Sheet Aeeva TE EA AE saves T EAA NATE AE O
38. Thermo Multiskan EX Thermo Scientific Multiskan EX Rev 3 3 Cat no 1507300 Thermo Scientific Multiskan EX Cat no 51118170 200 240 V or 51118177 100 120 V User Manual Rev 3 3 August 2007 Cat no 1507300 Copyright Copyright O 2007 Thermo Fisher Scientific All rights reserved Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks and Microtiter Multiskan and Wellwash are registered trademarks of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Contact information Thermo Fisher Scientific Oy P O Box 100 FI 01621 Vantaa Finland Tel 358 9 329 100 Fax 4 358
39. User Manual Rev 3 3 Cat no 1507300 20 Chapter 5 Installation The instrument operates at voltages of 100 120 Vac 220 240 Vac and the frequency range 50 60 Hz 5 3 Instrument layout and installation A Y t i AAA liy Instrument cover 1 2 Measurement assembly cover 3 Display 4 Keyboard 5 Printer optional 6 Retaining screws 2 of the instrument cover 7 Retaining screws 2 of the measurement assembly cover 8 Plate carrier 9 Transfer rails 5 Fig 5 2 Multiskan EX front view 1 Unpack the instrument See Section 5 2 1 2 Remove the two 2 instrument cover retaining screws Fig 5 2 item 6 using the Allen key provided and lift up the cover Install the filter wheel and the lamp See Sections 5 3 1 and 5 3 2 4 Connectthe printer or the computer according to the requirements See Fig 5 9 items 4 and 5 Caution DO NOT insert the computer s serial cable to the printer port This may cause unexpected problems 5 Checkthe baud rate transmit receive pin configuration and the handshake with DIL switches See Sections 5 4 2 5 4 3 and 5 4 3 6 Plug into mains See Section 5 3 3 7 Setup the instrument See Section 6 1 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 21 Chapter 5 Installation uuu Do DEED TM US us Pim IMA E Vim e Lamp Fan MUCEN circuit board Connector for Centronics interface optio
40. ad RxD 3 3 RxD E z S BS 232 5 RTS 4 4 RTS CTS 2 5 CTS LJ DSR 6 i DSR SIGNAL GND 7 SIGNALGND Ly DCD 8 8 DCD DTR 20 e 20 DTR Multiskan EX PC CHASSIS G NDL 1 CHASSIS GND 8765 a So Serial RS 232 switch RS CTS XA ant CIS DSR 6 6 DSR SIGNAL GNE Gender Standard D25 Laplink Cable Changer M M Multiskan EX PC CHASSIS G NDL 1 CHASSIS GND MEIN TD EV 2 TD n wo RO Serial RS 232 switch RIS z D CTS 5 XK 2 cts DSR 6 DSR SIGNAL GND Cable 230 3840 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 33 5 5 Computer Control Chapter 5 Installation In the primary ElA program you can use an external computer to substitute the keyboard use of the Multiskan EX Note Operate the instrument only with software and hardware specifically designed or selected for it The instrument does not verify the logic flow of the received commands Thermo Fisher Scientific assumes no liability for the use of third party software applications Computer control takes place via the RS 232C interface Start the computer mode by selecting measurement mode 6 computer control The computer can also start the computer mode by sending an R command after the instrument displays READY and the instrument has set the DTR to the high state Press either the stop key or send a Q command to return to keyboard use 5 5 1 Computer control commands In the
41. ananon 46 Infectious riaterials iscue iin eerta tai 82 Aare ASCIENDE a ci reia 18 21 SILC ice t etd ee e er Ere Pad ne d e a ele EAD a retire eben ere deed ed Gre EA LE E oa 20 Installing filter Wheel certe sciret E reb AE eL EDU ee Pu b LE RE Pul v PER E re PR E Eb RS 23 VSB osteo IDVun eed EE 24 Instrument advantag6s ii uenti eu 10 CLEANING id eo edic Lee ced etre teded 76 UU 21 decont armihatilon sei eese AAA i Pec Fa dae Ee Ov E EO CD D v E Ga ba ee Ed C 83 GiS POSAl Of esis 85 ATOMIC LGW AEEA E CU AA AA daa oie 21 IDSpecting IER etn Ee es i ee ed ii e HERES 18 20 internal VO Wisin A A ede nc dn o a e i A lv n A aa c 22 markings e eee dented ila ueniet Mu 91 PACKING TOM SERVICE ica e a eei eeni A a dad 85 mum H 27 Ul packiliQg is eterno irte ei Ed euro ti ie ru ee ieee eee deir 19 Intendediuse s dioe tos AA ce ra eer a re ape ta dr Cera dr d Re n v EAA cea a Qr e dd 10 IMtEPFACES CONNECTIONS a a a aaa avenonevanctacsvaseneves ncuseadvasabdeuwacwelenscenoverenetes 33 Interlace Sy Store ie S PERI did 28 Interference f E a r rra a na are aa eaaa a dda n aran 11 12 Internal printer IAN PAP EE ee E T RE eee 85 Select iii a 47 StOPS pfrihting o et itt ri Ad ri 86 Interva l time 4o edt ee e EH Pvt O dr ER ere Ea T Ee did 52 Thermo Scie
42. bance values of the standards are given and a standard curve is plotted The calculated values of the individual samples are given in plate layout format Print the raw measurement data READY Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 75 Chapter 7 Maintenance 7 1 Regular and preventive maintenance 7 1 1 Service contracts It is strongly recommended that this instrument be regularly maintained and serviced every twelve 12 months on a contract basis by trained service engineers of the manufacturer This will ensure that the product is properly maintained and gives trouble free service For more details contact Thermo Fisher Scientific s service department 7 1 2 Maintenance check list The check list below contains a recommended maintenance schedule Table 7 1 Maintenance check list Item xo o E gt gt d E I 5 8 a o 0 5 2 gt Wipe the case of the instrument e Cover the instrument with the dust cover after use e Clean the instrument outside the transfer rails and e track Fig 5 2 item 8 and the plate carrier Fig 5 2 item 9 with a cloth dampened with mild detergent followed by deionized distilled aqua Clean and check the condition of the filters and the 2x filter wheel position slots Clean the optical system See Section 7 3 If the internal printer is installed change the paper roll e when paper is finished See Secti
43. c s service department Check Section 7 10 You will find instructions on how to proceed before shipping the instrument for service Check that any necessary decontamination procedure has been carried out before packing See Section 7 8 Decontamination procedure Ensure that the Certificate of Decontamination see Appendix B as well as the return authorization number RGA are sent with the instrument Thermo Fisher Scientific s service department will keep you up to date with the progress of service and provide you with any further details you might need e g on maintenance serviceability troubleshooting and replacement 8 3 Warnings and cautions 8 3 1 Electrical A Thermo Scientific Multiskan EX User Manual This instrument is designed to provide full user protection When correctly installed operated and maintained it will present no hazard to the user The following recommendations are given for added user safety Ensure that the mains supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities The mains plug should only be inserted into a socket outlet provided with a protective ground contact Never use an extension cable without a protective ground wire DO NOT replace fuses without first disconnecting the mains supply cable Warning Rev 3 3 Cat no 1507300 92 Chapter 8 Troubleshooting Ensure that only fuse
44. consumable It is recommended that you check its condition twice a year to ensure proper functioning of the instrument It can be checked using the Plate verification PVT kit 7 5 Changing the lamp If the lamp burns out replace it as follows Warning Only use the lamp approved by the supplier Cat no 2400620 Lamp quartz tungsten halogen lamp Osram 64607A 8V 50W 1 Turn the power OFF and unplug the instrument Open the instrument cover by unscrewing the two 2 cover retaining screws on each side of the instrument and lift up the cover Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 83 Chapter 7 Maintenance lamp the lamp and its surroundings may be very hot Wait for the lamp Warning If the instrument has been in use and you need to replace a burned to cool down before replacing it Caution When handling the lamp do not touch any other mechanical or electronic part 2 Liftup the lamp with the terminal socket Pull the terminal socket from the lamp contacts Fig 7 7 Fig 7 7 Changing the lamp Caution DO NOT touch the reflective surface of the new lamp or the bulb itself 3 Refit the terminal socket to the contacts of the new lamp approved by the manufacturer OSRAM 64607A 8V 50 W Place the new lamp into its place 4 Close the instrument cover and replace the two cover retaining screws Plug in the instrument and switch the power ON Thermo Scientific Multiskan EX Use
45. d set by the manufacturer down disabled When the DSR handshake is switched OFF the Multiskan EX does not test the DSR before sending each character The state of the DTR pin 20 can always be checked by a computer regardless of the fourth DIL switch Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 30 5 4 4 Transmit receive pin configuration Chapter 5 Installation In order to make a connection between the Multiskan EX and the computer easier pin 2 can be selected to transmit or receive and pin 3 to do vice versa The configuration is selected through the DIL switches 5 6 7 and 8 Fig 5 13 Fig 5 13 DIL switch positions 5 6 7 and 8 for transmit receive selection Table 5 4 DIL switch positions for transmit receive selection Switch position Transmit receive pin 8 7 6 5 up up down down pin 2 transmits and 3 receives set by the manufacturer down down up up pin 2 receives and 3 transmits 5 4 5 Parallel Centronics type printer interface Data transfer via a parallel Centronics type printer interface is performed through the Centronics interface Fig 5 9 item 4 When using the Centronics interface the secondary interface DIL switches 1 2 and 3 Fig 5 14 should be positioned as follows Fig 5 14 Secondary DIL switch positions 1 2 and 3 for Centronics type printer interface Table 5 5 Secondary DIL switch positions for Centronics type p
46. d other foreign matter e Avoid touching the lens surfaces filters or detectors with your bare fingers e Perform the operational test regularly see Section 5 4 For reliable daily operation keep the instrument free of dust and spills from liquids It is also advisable to cover the instrument with the dust cover supplied when not in use In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance see Section 7 2 Clean the keyboard surface with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 8 7 1 4 Immediate Although the Multiskan EX is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled aqua 7 1 5 Filter maintenance The filter is a consumable It is recommended that you check its condition twice a year to ensure proper functioning of the instrument It can be checked using the Plate verification PVT kit For ordering information
47. dwidth of filters 3 9nm Wavelength accuracy 2nm Detector Eight 8 silicon photodetectors Measurement range 0 3 5 Abs Resolution 0 001 Abs Linearity 0 2 Abs 2 0 at 405 nm Accuracy 2 0 or 0 007 Abs whichever is greater typical value 1 0 2 0 Abs at 405 nm Precision CV lt 0 5 0 3 1 5 Abs at 405 nm CV lt 1 0 1 5 2 Abs at 405 nm Start up time One 1 minute Measurement time 5 s 96 well plate continuous mode 20 s 96 well plate stepping mode Long term stability Due to the autoblanking procedure the instrument is stable Shaker Linear shaking 3 speeds Display 82 mm L x 19 mm W 2 x 20 character alphanumeric LC display 5 x 7 dots per character Keyboard 30 keys with click action switches Onboard software Primary ElA Cutoff and Cubic spline calculation programs Reader memory RAM 32 kB ROM 64 kB for each program module max 5 x 64 kB EEPROM 32 kB Programs 64 user determined programs Interface RS 232C serial interface Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 94 Chapter 9 Technical Specifications General specifications Centronics type parallel printer interface Integrated printer Three 3 columns simultaneously up to 0 000 3 decimals optional Resolution 138 dots per line Speed results of one 96 well plate printed in about 60 s 9 2 Safety sp
48. e SBS standards The Multiskan EX can be used in research or routine test laboratories by professional personnel For verification of the entire system it is recommended that Good Laboratory Practices GLP be followed to guarantee reliable analyses Use for self testing is excluded If the analyzing performance is critical to the medical diagnosis the diagnostic test result has to be ensured with internal quality contols or with an alternative test 3 2 Principle of operation The Multiskan EX is an eight channel vertical light path filter photometer designed to perform standard photometric measurements The versatile onboard software of Multiskan EX allows end point and kinetic reading modes Flexible cutoff calculations and curve fit algorithms give Multiskan EX increased flexibility for data manipulation in various diagnostic applications The extended memory holds up to 64 assay protocols It comprises the following keyboard selectable software packages program modules Primary EIA Cutoff and Cubic spline Each package is a combination of different measurement and calculation modes see Section 4 2 Each mode is self prompting which reduces the error factor and simplifies operation The wavelength 400 750 nm is selected using maximum eight high quality interference filters held in a filter wheel Standard filters i e 405 nm 450 nm and 620 nm are included in the Multiskan EX The readings can be processed via a serial
49. e cxtetetecteieianetesdieare 98 Glossary and Abbreviations eiii i eaa ca sasestbeanedenteschivsuediveestess 105 Keywords for web pages ccccococcccnononccnnononcnnnnnnn nn nnnnnn nn nn nn anna KREANTE nnne nnne nenne nnns Ea 106 Thermo Scientific Multiskan EX Quick Reference Guide 107 Certificate of Decontamination oooococccccnncononocnnncncnnnnnnannnnnnnncnnncnnanan ano nn hann nnn nana 109 Thermo Scientific Multiskan EX Feedback Form eeeeeeneeeeeeeree 110 Addresses A RR AT 111 Drs M 112 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 6 List of Figures Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 4 1 4 2 4 3 4 4 4 5 5 1 5 2 5 3 5 4 5 5 5 6 5 7 5 8 5 9 5 10 5 11 5 12 5 13 5 14 6 1 6 2 6 3 6 4 7 1 7 2 7 3 7 4 7 5 7 6 7 7 7 8 List of Tables Table 4 1 Table 4 2 Table 5 1 Table 5 2 Table 5 3 Table 5 4 Table 5 5 Table 5 6 Table 5 7 Table 5 8 Table 6 1 Table 6 2 Table 6 3 Table 6 4 Table 6 5 Table 6 6 Table 6 7 Table 6 8 Table 6 9 Table 7 1 Table 7 2 Table 8 1 List of Figures and List of Tables Optical SVSlOIm s e t Es 11 Flat and concave liquid surfaces an ld Cal
50. ecifications Safety performance EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 including CENELEC Common Modifications US and CA National differences EN 61010 1 2001 Ed 2 9 3 In conformity with the requirements Multiskan EX bears the following markings Type 355 100 120 Vac 200 240 Vac 50 60 Hz 170 VA CE Multiskan EX conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B 2002 96 EC Waste of Electrical and Electronic Equipment EMC performance EN 50081 1 1992 Generic emission standard Residential commercial and light industry EN 50082 1 1997 Generic immunity standard Residential commercial and light industry EN 61326 1 1997 A1 1998 Product family standard Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 95 Chapter 10 Ordering Information 10 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Code 51118170 Instrument Multiskan EX 200 240 V 51118177 Multiskan EX 100 120 V 51118060 Onboard printer for Multiskan EX 51118070 51118080 Cutoff option for Multiskan EX Cubic spline option for Multiskan EX 10 1 List of accessories Code 2304040 Item RS 232C interface cable for prin
51. ect sunlight draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables reagent bottles etc e Leave sufficient space at least 10 cm on both sides and at the back of the unit to allow adequate air circulation e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make sure the ambient temperature range is between 10 C 50 F and 40 C 104 F e Make sure relative humidity is between 10 and 90 non condensing The Multiskan EX does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Warning DO NOT operate the instrument in an environment where potentially damaging liquids or gases are present 5 2 5 Things to avoid DO NOT smoke eat or drink while using the Multiskan EX Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Use proper protective clothing Use disposable gloves Ensure that the working area is well ventilated Never spill fluids in or on the equipment Only use the instrument for the purpose it is intended for Refer to Section 3 1 5 2 6 Technical prerequisites Place the instrument on a normal laboratory bench The net weight of the instrument is 11 kg 24 Ibs Thermo Scientific Multiskan EX
52. ent MOE eret rece EL tege ela casted tae tip eer de ceed 51 Measurement parameeters sssssssssesseeseeee aa Tap nennen enin nh netnta inet enin nnne nete s aen en nnne nete rennen nns 51 dealt Val ES ina 16 Measurement priniciple e cito ree a A XE Eee Exec e gees REPRE 11 Measu rement range eerte A re tarde teed i ia 90 Microplate disposal Ofen ete tere cte tese ctr t teret esed A E o vus s Eae nin Letra DEO Te Enos ir EE M E GE 82 DANI A neuen hee 42 Microstrip disposal Mi oar eese aad EE dira Fo oO a ad drm de exa Beas aia 82 MIDA ciao dada abran 49 58 59 Module listirig 5r ae 58 MUCEN circUiEboard cti ale ca dc di db a EAEEREN 22 Multiwavelength measurement mode sss rr enn 51 MUSCU 2A processor circuit board nennen enne enne nnne enne esent nn 22 N NAPSU2 keyboard circuit board cccccconnnocconcnncnnnannonononnnnnnnnnonnnnnnnnnnncnnnnnnnnnnnnnnnnnnnnn nn rre anis a sas nns sanas 22 Negative lenis effect s ince ot e e nhe eei e Ee RE ae ER RAE CEN ERA e Fed ENER RN e ERR id 14 No Calcula TORRES DE 53 55 57 No Centroriics pFibteEs cii cete eren add cl EE EEA A Ac 87 Dfeai oe E EET 87 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 101 Chapter 12 Index INO DOW get eR 86 Number otitis ii 58 59 O Operating conditiONS iia di a dais 20 Operation efe eim Lm 39 principle ioter eec e
53. ente deci oe dc Eee det doeet be Eder tetuer eei Ed det 10 Operational Chleck coo erre oe RE a 38 Optical fiber bundle oce reet aer b cnr dic 11 Optical SySteli uer icitoe ursa Eee tinere ne oe hne re Ye Eve EE Eel re fe Eg haa ao rre Flea ve ee ke Eva Ee ne Ele re nde Ee RE Beet 11 Orderingntormation odose eviter elles e dme ads 92 Out Of Tangerine err ee lacs ta ce e ERR a E 87 P Packing disposal of materials A ie ra dip ERR TO EE Rie eves ane RET ARR Ga aii 85 TOI SOV IC Crees vacate iia Per CER UNDER POTE Dev CER RU ROOTED eve EDEN 85 tiep P mEM m 18 19 Paperroll EE 85 Parallel Centronics Interface errechnet racha Eran RR Fa re FECE Ea RR TAN EAE EnA REKRA EORR E eR TER 28 Param key ERREUR 57 Plate E T e iin it Eo a inh e par aes o Edu take Fra a fu aiid Pe Eoi cer aves ao Fere reda e roba vw E 10 46 Callers shires Reece A eer eto eeu ated eae eles Gi na 46 Plate analVsiS ac a diia 58 59 Plate CAB ts 21 76 adjusting a ED e Re e litem reme A a TET 26 Plate OTRO E E 87 Plate movement 55 inwganieiien iere eret cis D Gc d Da pre CE ER Ee add Te Dra Le PE dea a o d 57 59 Pintto ponta cede PEEL 55 Power CORSUITptlOn e a ai 90 lide 90 PE MIO lirica alii T 49 58 59 Primary ElA program module acknowledgement MessagBS cccccccncnnononcnncnnnnnn nora 36 calculation parameters diia ii a a 53 EXAM pleito e a 60 Printer On Off Selections ii a 57 59 Printere Oresa condotte ee
54. ews Fig 5 2 item 7 of the measurement assembly cover and remove the cover Clean the track Fig 7 1 item 4 inside the measurement assembly and the eight 8 focusing lenses at the end of the optical fiber bundle Fig 7 1 item 3 as mentioned above Caution DO NOT touch the measurement circuit board Fig 7 1 item 1 by hand 5 The upper lenses are situated under the detector frame Fig 7 1 item 3 Remove the two 2 detector frame fasteners with washers Fig 7 2 item 1 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 86 Chapter 7 Maintenance Lift the detector frame from the handle Fig 7 3 item 1 Clean the upper lenses Fig 7 3 item 2 in the same way as the focusing lenses Caution DO NOT use acetone to clean the plastic lenses focusing lenses or upper lenses Avoid harsh treatment 6 Let the surfaces of the instrument dry 7 Wipe out the thin film caused by the decontamination agent from the surfaces of the eight 8 focusing lenses and the upper lenses with a napless lens tissue dampened with water Dry the lenses with a dry lens tissue 8 After drying the upper lenses lower the detector frame back to its position Caution Make sure that the Hall element sensor Fig 7 3 item 3 is positioned into its slot Fig 7 3 item 4 9 Replace the detector frame fasteners with washers Fig 7 2 item 1 Make sure that the detector frame fasteners are placed so that the edge in the
55. f the concave liquid surface 3 Standard curve due to negative lens effect of the concave liquid surface Fig 4 3 Calibration curves The concave surface of the solution causes another effect also which will affect photometry This effect arises from the fact that the concave surface functions as a negative lens Fig 4 4 The cone of the light leaving the surface will be wider and some of the light is diverted enough to bypass the detector Surfaces with identical curvature will cause equal losses of light at the detector and equal increase in the apparent absorbances at all absorbance values The absorbance will be increased by an additive amount and the standard curve will have the same slope as the actual curve but intersects the absorbance axis above the origin as shown in Fig 4 3 item 3 i Fig 44 Concave liquid surface acting as a negative lens Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 14 Chapter 4 Functional Description In practice this phenomenon must be taken into account in all end point determinations but not however in two point or kinetic determinations where the result is based on the absorbance difference The correction is made by using the blank cuvette block with a similar surface to the analysis cuvettes Because of the difference in the slopes obtained by flat and concave surfaces it is important to have identical shapes of liquid surfaces in all cuvettes including blank sta
56. fiber bundle Focusing lenses Upper lenses and detectors oo O e W N gt Fig 4 1 Optical system Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 11 Chapter 4 Functional Description Light source The light source is a quartz tungsten halogen lamp Osram 64607A 8V 50W equipped with an aluminum coated elliptical reflector To prolong the useful life of the lamp switch off the instrument when it is not in use A warm up period of one 1 minute has to be allowed before you measure Chopper wheel The chopper wheel chops the light beam to minimize electronic noise Semitranslucent mirror After passing through the condenser lens the light beam impinges on the semitranslucent mirror Part of the visible light and all longer wavelengths pass through the mirror and the UV whereas the rest of the visible light is reflected This arrangement eliminates heating the interference filter and evens out the spectral intensity distribution Interference filter The wavelength is selected from one to eight 1 to 8 filters held in the filter wheel Optical fiber bundle After passing through the interference filter the light beam reaches the common end of the optical fiber bundle that refracts the beam into eight 8 equal parallel beams and deflects the beams upwards Focusing lenses After being refracted the light passes through a focusing system of eight 8 lenses Detectors The light beams pass thro
57. fic Multiskan EX User Manual Rev 3 3 Cat no 1507300 47 6 8 2 Selecting the plate movement 1 CONTINUOUS MODE Thermo Scientific Multiskan EX User Manual 2 PLATE MOVEMENT 2 STEP MODE READY Rev 3 3 Cat no 1507300 Chapter 6 Operation 48 Chapter 6 Operation 6 8 3 Selecting shaking men D 1 MIXING OFF READY 2 MIXING ON No shaking selected 1 MIXING FAST 2 MIXING MEDIUM 3 MIXING SLOW MIXING TIME 15 READY Shaking selected Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 49 Chapter 6 Operation 6 8 4 Entering filter wheel parameters An example of how to enter the filter wheel parameters in the memory of the embedded software Press 1 RS FORMAT 6 FILTER WHEEL NR OF FILTERS 3 Enter the total amount of filters in the filter wheel including earlier installed filters b DO NOT change remove the factory earlier entered information FILTER 1 WAVEL 405 FILTER 2 WAVEL 450 Depending on the earlier installed amount of filters Press FILTER 3 WAVEL 620 FILTER 4 WAVEL Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 50 Chapter 6 Operation Enter the wavelength for Example the first optional filter FILTER 4 WAVEL 414 Enter the wavelengths tor JAR LLL ccc Continues depending on the other optional filters 5 8 in the same manner how many add
58. fter the earlier installed filters Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 82 Chapter 7 Maintenance Table 7 2 Example of the filters in the filter wheel positions 1 8 Filter wheel position Wavelength nm 405 example of factory installed filter 450 example of factory installed filter 620 example of factory installed filter OINI AJOJN 6 Write down in Table 7 2 the additional filters you have installed into the filter wheel It is recommended to add the filters in ascending wavelength order Place the filter spring into its original position and fasten the screw 8 Slide the filter wheel back into the filter wheel slot with the toothed edge facing towards the rear of the instrument The magnet locking mechanism will automatically lock the wheel in the correct position See Section 5 3 1 9 Close the instrument cover and replace the two cover retaining screws 10 Switch the instrument ON 11 Add the filters into the embedded software s general instrument parameters filter wheel number of filters and wavelength using the param key See Section 6 8 4 and Table 6 8 Note The measurement results will be incorrect if the filter parameters differ 1 from the actual filters on the filter wheel 12 Perform the operational check to ensure the correct functioning of the new filter Section 5 6 13 Now the filters are ready for use The filter is a
59. hat the Multiskan EX was not able to understand The P and PM commands are acknowledged by sending values for the measured wells The data flow to the computer is a follows DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA DATA CRLF where CRLF carriage return and line feed and DATA 6characters where the first character can be a space or a minus sign and the remainder can be digits or decimal points The V command sends the text MULTISKAN VERSION X Y where X can be 1 9 and Y can have 1 to 8 characters as an acknowledgement message The T command is acknowledged by sending XX XXX 20XX XX XX XX example 19 MAR 2003 20 42 03 The N command is acknowledged by sending SERIALX X where X can be 0 9 or space example SERIAL352 00001 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 36 5 5 3 Availability of computer control commands Chapter 5 Installation Table 5 8 Availability of comp
60. have been misused or abused For the warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e Any defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the character listed above e Refurbished products that are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for any loss or damage arising out of or in connection with the use of the product or other indirect damages Full warranty terms and conditions can be obtained from your local Thermo Fisher Scientific dealer This document acts as a warranty certificate 11 1 Warranty limitations Consumables are not included in the warranty Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 97 Chapter 12 Index A ADSOrDANG E uaren a a aaa 12 Absorbance measurement Mode ccccccccnconononcncninnno conan 51 ACCESS OOS P TR DEO D D DEED ULLUS 92 hielt Em 18 20 WIMP ACK G EUER 19 DtdUrucl e
61. hnical prerequisites Section 9 1 Avoid sites of operation with corrosive vapors smoke and dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations Leave sufficient clearance at least 10 cm on both sides and at the rear of the unit DO NOT operate the instrument in an environment where potentially damaging liquids or gases are present Remove the two 2 instrument cover retaining screws Fig 5 2 item 6 using the Allen key provided and lift up the cover Install the filter wheel See Section 5 3 1 Install the lamp See Section 5 3 2 DO NOT touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty DO NOT touch the measuring circuit board by hand Connect the printer or the computer cables according to the requirements See Fig 5 9 items 4 and 5 DO NOT insert the computer s serial cable to the printer port This may cause unexpected problems If applicable check the baud rate transmit receive pin configuration and the handshake with DIL switches See Section 5 4 Connect the mains supply cable to the mains input socket See Section 5 3 3 Set up the instrument See Section 6 9 3 Perform the operational check See Section 5 6 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 18 Chapter 5 Installation 5 2 What t
62. i At dd 87 PrinterODVOff iii a rena D D e iaa 47 Printing program modules 5 n VERSES 58 progra Sie E 57 Printout spacing ipte lada oo que seva ru Riga 47 58 59 Program module NUMbDer id eri 60 Program Modules cit iia 15 DEI ca AAA AA Aa e eddie 58 Program PLATINO A A A ed 57 Programming examples Cubic spline program module seesseeeseseeee eene nn nr naar nn nr ran nn nr rana nennen entrain 69 Cutoff program module dai n ne edi edad tio iii 65 primary EIA program modulen crani ta A A A td 60 Protective Conductor o A A BERE sa genset ets 27 Q Quartz halogen lIarmps cccid oce cede doc eerte tena toe eve tende eee d vtt en eb e eo dee vg 11 Quick Reference Guide 5 3 nit onte err EAE rra mk ais 103 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 102 Chapter 12 Index R Range calculation iria adan Dead reae 54 56 Read outrange 2 20 A AR et eee 90 recall key roue a n EE ees ERE E 42 Replacing fUuS683 c dtes AA ie dg rae des Te ER A RS RE RR EER ecoute Ai 82 Elio RES 81 paper roles pepe epe nU sU aaa 85 Retained blank cia a tear rin 52 RetainiIng SCreWS a a 21 RS TOL ia RAS EA ene ene A his oi a rtu 57 59 icol TI EP EE 47 RS 232C interface iuc cote Ee pin ke eta XE EE ERE Eae T E AR ae 28 RS 232C interface connector iie Lr arr naci dd E na ara eR Rana dn 27 S
63. iDratlOD CUEVOS oui frat ae ip oco oto d to e geo si 14 Concave liquid surface acting as a negative lens es 14 MultiSkatni EX keyboard ae is t Ate reis AR ee ale 17 Optional printer as delivered all parts not viSIblE oocooocccocninoniconononinininininos 19 Multiskan EX TroDU VI W iai in pr pe enata Multiskan EX internal view Multiskan EX fille whel ists cscs i esee tes a poe to AO LAE bd b ee Placing the Multiskan EX filter wheel into the filter slot ud Fit ng the amp contacts i tas de banat cei Deor eo uir t ddr and 24 SR ELA CP 25 Adjusting the plate carrier 26 Mu ltiskan EX TBaFWIGW eea ea vs ru a UR UR ER Ed ERN ona Re 27 Factory set DIL switch positions viewed from the rear as shown in Fig 5 9 28 DIL switch positions 1 2 and 3 for baud rate selection 29 DIL switch position 4 for handshake selection 30 DIL switch positions 5 6 7 and 8 for transmit receive selection ssus 31 Secondary DIL switch positions 1 2 and 3 for Centronics type printer interface 31 Positioning the microplate correctly annii hinh hahaha aaa 43 Plate layout in the example for Primary EIA program module 2 62 Plate layout in the example for Cutoff program module sees 67 Plate layout in the example for Cubic spline program module sss 71 Opening the measurement assembly cover ni LE Opening the detector frame uu 80 Lifting the detector frame
64. ide and error messages sssssssssssssssssseeeee nn Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 7 Chapter 1 Safety Symbols and Markings 1 Safety Symbols and Markings These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated Safety symbols and markings used on the Multiskan EX Power ON C Power OFF SN Serial number REF Catalog number Date of manufacture Consult Instructions for Use WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC P x Warning markings used in the documentation Warning Risk of electric shock AN Warning Biohazard risk Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of N performance or function in a specific application Other markings used in the documentation Note Marks a tip important information that is useful in the optimum 1 operation of the system or an item of interest Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 8 Chapter 2 About the User Manual 2 About the User Manual This User Manual has been written for the actual user e g laboratory technician and provides information on the Thermo Scientific Multiskan EX photometric microplate absorbance reader
65. ime as HH MM SS HH hours MM minutes SS seconds results are transmitted via the RS 232C interface the actual minimum A Caution When the kinetic measurement and ALL RESULTS are selected and the interval time is dependent on the baud rate used the lower the baud rate the slower the intermediate results are transmitted This means that the interval time has to be long enough for the instrument to be able to transmit the intermediate results during the selected interval time Example with baud rate 4800 the minimum interval time is 6 7 depending on the printer used LAG TIME You can select the LAG TIME parameter in kinetic measurements It is the time before the measurement is started The minimum lag time is 0 s the maximum 17 h 59 min 59 s with Primary EIA and 100 s with Cutoff and Cubic spline program modules Enter the lag time as HH MM SS HH hours MM minutes SS seconds TOTAL TIME You can select the TOTAL TIME parameter in kinetic measurements It is the time during which the reaction is followed The minimum total time is 5 s the maximum 17 h 59 min 59 s with Primary EIA and 100 s with Cutoff and Cubic spline program modules Enter the total time as HH MM SS HH hours MM minutes SS seconds Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 54 6 9 2 Calculation parameters 6 9 2 1 Primary ElA program Chapter 6 Operation Table 6 5 Calcu
66. istic chemical reaction in a sample that allows an analyte to be detected and measured RS 232C ElA approved standard used in serial data transmission covering voltage and control signals The data is transferred serially one digital bit at a time via one path but some control signals can be transferred simultaneously via parallel paths 13 1 Keywords for web pages absorbance column subtraction cubic spline cutoff double limit drug discovery dual wavelength EIA ELISA end point enzyme immunoassay factor immunoassay kinetic reading liquid handling limit Thermo Scientific Multiskan EX User Manual linear standard microplate Microtiter plate microwell plate multiwavelength photometer photometry plate point to point primary EIA range sandwich assay standard line Thermo Scientific Thermo Fisher Scientific two point Rev 3 3 Cat no 1507300 106 Appendix A Multiskan EX Quick Reference Guide Thermo Scientific Multiskan EX Quick Reference Guide Use the power switch at the rear of the instrument Fig 5 9 item 1 Switch the instrument on To prolong the useful life of the lamp switch off the instrument when itis not in use A warm up period of one 1 minute has to be allowed before you measure Wait one 1 minute 1 PRIMARY EIA i 2 CUTOFF READY Select first the 3 CUBIC SPLINE o oem o Take the stored program into use Place the microplate onto the plate
67. it a positive sign is sent The measurement parameters calculation parameters and the results in a 0 matrix are sent via the interface Parameters Low limit value LOW LIMIT 0 000 2 000 High limit value HIGH LIMIT 0 001 2 000 7 RANGE CALCULATION 8 COLUMN SUBTRACTION The range value defined by the user is divided into ten 10 equal segments The segments are numbered from 0 to 9 The measured absorbance value is represented by one of these numbers depending on which segment it lies in The measurement parameters calculation parameters and the matrix where the results are shown by segment numbers 0 9 are sent via the interface Parameters Range value RANGE 0 000 2 000 Example if an absorbance range of 1 5 is set the segments are as follows Segment Measured absorbance value in range Printout A x 0 150 0 150 A x 0 300 0 300 A x 0 450 0 450 A x 0 600 0 600 A x 0 750 0 750 A x 0 900 0 900 A x 1 050 1 050 A x 1 200 1 200 lt A lt 1 350 10 1 350 lt A lt 1 500 The results of column 1 are subtracted from the results of column 2 the results of column 3 are subtracted from the results of column 4 etc or vice versa The measurement parameters calculation parameters and the absorbance differences of every other column are sent via the interface COONDOOARWNDM OANDARWN O Parameter 1 2 3 4 or 2 1 4 3 Continued Thermo Scientific Multiskan
68. itional filters are to be entered PERFORMS AUTOCALIBRATING READY Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 51 6 9 Programming parameters of the Multiskan EX Chapter 6 Operation Measurement modes meas mode key Measurement parameters meas param key Calculation modes and parameters calc mode and calc param keys Primary ElA Cut Off Cubic Spline General parameters param key 1 ABSORBANCE MODE 2 DUAL WAVEL MODE 3 TWO POINT MODE 4 KINETIC MODE 5 MULTI WAVEL MODE 6 COMPUTER CONTROL FILTER 1 NO REAGENT BLANK 2 SINGLE WELL BLANK L BLANK WELL A1 3 COLUMN MEAN BLANK L BLANK COLUMN 1 4 COLUMN BLANK L BLANK COLUMN 1 5 ROW MEAN BLANK L BLANK ROW A 6 ROW BLANK L BLANK ROW A 1 BL ON EVERY PL 2 RETAINED BLANK L 1 USE OLD BLANK 2 NEW BLANK NOW 1 END RESULTS 2 ALL RESULTS INTERVAL TIME LAG TIME TOTAL TIME 1 NO CALCULATION 2 FACTOR CALCULATION L FACTOR 3 LINEAR STANDARD L NO OF STDS L NO OF REPL L STANDARD 1 L STANDARD 1 REPL 1 A1 L 1STD ON EVERY PLATE 2 RETAINED STD L 1 USE OLD LINE 2 NEW LINE NOW 4 STANDARD LINE L LINE SLOPE L INTERCEPT 5 LIMIT CALCULATION L LIMIT 6 DOUBLE LIMIT L LOW LIMIT L HIGH LIMIT 7 RANGE CALCULATION L RANGE 8 COLUMN SUBTRACTION L 1 2 3 4 5 6 2 1 4 3 6 5 9 POINT TO POINT L NO OF STDS L NO OF REPL L STANDARD 1 L STANDA
69. kaging e Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges e Enclose a dated and signed Certificate of Decontamination Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s service department See Chapter 9 for details on storage and transportation temperatures See also Section 8 2 Service request protocol 7 11 Disposal of the instrument Warning Decontaminate the instrument prior to disposal See Section 7 8 and Appendix B on decontamination Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Fisher Scientific products which may assist the detection of substances s
70. l contains all test reagents except the sample itself The absorbance of the reagent blank is usually subtracted from all other absorbances Specific blank each calibrator control or sample has its own blank A specific blank well contains the sample and the buffer but not the reagent giving the specific reaction Background caused by individual samples can be subtracted Abbr Bl BL or BLANK Control s A material solution lyophilized preparation or pool of collected serum designed to be used in the process of quality control The concentrations of the analytes of interest in the control material are known within limits ascertained during its preparation and confirmed in use Cubic spline Cutoff A curve fit type Spline curves of degree 3 provide a useful means of approximating data to moderate accuracy Cubic spline uses repeated calls to the cubic polynomial fit using three sequential calibrators A spline is a curve calculated by a mathematical function that connects separate points with a high degree of smoothness The limit or threshold value In qualitative assays cutoff can provide you with interpretations of data in a qualitative way e g positive negative high or low DIP switch One or more small rocker or sliding type switches contained in the plastic or ceramic housing of a dual in line package DIP connected to a circuit board Each switch on a DIP switch can be set to one of two positions closed o
71. lation parameters in Primary EIA program Primary EIA program calculation parameter Operation 1 NO CALCULATION The results are sent as absorbances via the interface 2 FACTOR CALCULATION Measured absorbances are multiplied with a factor given by the user to obtain the concentrations The concentration unit is defined by the unit for the factor The concentration is calculated according to the following equation Concentration 2 FACTOR x absorbance The measurement parameters calculation parameters and the calculated concentrations are sent via the interface Parameter FACTOR 3 LINEAR STANDARD 4 STANDARD LINE 5 LIMIT CALCULATION Enter the concentrations of the standards and the Multiskan EX calculates a standard line with the aid of these standards using the method of least squares The measured absorbances are converted to concentration units using this line The measurement parameters calculation parameters and the concentrations obtained are sent via the interface Parameters Number of standards NR OF STDS Number of replicates NR OF REPL Standard concentrations STANDARD 1 etc Standard locations STANDARD 1 REPL 1 A1 etc Up to eight 8 standard solutions can be incorporated into the plate in up to three 3 replicates 1 STD ON EVERY PLATE Standard on every plate 1 STD ON EVERY PLATE or 2 RETAINED STD retained standard 2 RETAINED STD the standard curve of the former plate is
72. les on how to use the Multiskan EX 6 10 1 Example 1 Primary ElA program module e Module 1 primary EIA Note The program module number depends on which modules are installed into your Multiskan EX instrument It may therefore differ from the program module number given in this example e Absorbance measurement with point to point calculation Three 3 standards with concentrations 0 15 and 100 and in positions B1 C1 and D1 Two 2 replicates positions B2 C2 and D2 on every plate The blank is positioned in well A1 on every plate The results are printed out on the internal or external printer or the computer according to the selection Section 6 8 1 1 2 3 4 5 6 7 8 9 10 11 12 A Blank Un 5 Un 5 B Std 1 Std 1 etc etc C Std 2 Std 2 D Std 3 Std 3 E Un 1 Un 1 F Un2 Un 2 G Un 3 Un 3 H Un4 Un 4 Fig 6 2 Plate layout in the example for Primary EIA program module Switch the instrument on Wait one 1 minute Select the program module 1 PRIMARY EIA READY Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 62 Chapter 6 Operation Select the meas measurement mode mode 1 ABSORBANCE MODE Select the meas param measurement parameters FILTER 340 Enter the wavelength of the filter or the filter position number FILTER 450 1 NO REAGENT BLANK 2 SINGLE WELL BLANK Position of the blank BLANK
73. meters Select the calculation mode READY 4 You have now made a program Save it to the instrument memory on READY 5 Select shaking if required See Section 6 8 3 on p 49 and Table 6 8 on p 59 Also see examples in Section 6 9 1 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 108 Appendix B Certificate of Decontamination Certificate of Decontamination The decontamination procedure is required prior to shipping the instrument to Thermo Fisher Scientific Oy e g for repair If for any reason the instrument is shipped back to Thermo Fisher Scientific Oy it must be accompanied by a dated and signed Certificate of Decontamination which must be attached to the outside of the package containing the instrument See Section 7 8 Decontamination procedure Failure to confirm decontamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrument s or item s ensure that they are fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals
74. microplates The plate carrier has been adjusted for the Microtiter 96 well plates However each manufacturer s plates have slightly different dimensions The plate carrier can therefore be adjusted to suit the used plate type Take into account he following considerations e The plate must not move in the x direction at all When the Multiskan EX is used for shaking the samples if there is even a small gap the plate may spill the contents e You must be able to insert the plate into the plate carrier easily without using force This is especially important when using robots e Both ball screws must be level The plate may otherwise stay tilted especially when using robots Adjust the plate carrier using the screws on the right side Fig 5 8 Ball screws Fig 5 8 Adjusting the plate carrier Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 26 Chapter 5 Installation 5 3 4 Mains supply cable connection Never use a mains supply cable other than the Thermo Scientific mains supply cable designed for your region Make sure that the protective conductor inside or outside the instrument is never interrupted and that the protective conductor terminal is not disconnected Warning Never operate your instrument from a power outlet that has no ground 1 Ensure that the mains switch Fig 5 9 item 1 at the back of the instrument is in the OFF O position 2 Connect the mains supply cable to the mai
75. nal RS 232C interface or optional IEEE 488 interface MUSCU 2A processor circuit board Liquid crystal display LCD circuit board NAPSU2 keyboard circuit board SCANPRI2 printer circuit board optional Connector for built in printer optional 10 Filter wheel 11 Filter wheel slot 12 Chopper com 00 xog Fig 5 3 Multiskan EX internal view Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 22 Chapter 5 Installation 5 3 1 Installing the filter wheel 1 Unpack the filter wheel It is delivered in a filter wheel box Check that all filters are clean and undamaged Fig 5 4 Multiskan EX filter wheel Caution DO NOT touch the filters with your bare fingers Caution When installing the filter wheel and the lamp do not touch any other mechanical or electronic part 2 Place the filter wheel into the filter slot Fig 5 5 so that the toothed edge faces towards the rear of the instrument A magnet locking mechanism will automatically lock the wheel in the correct position and the optical filter position sensor will make sure that the correct filter is used during measurement Fig 5 5 Placing the Multiskan EX filter wheel into the filter slot Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 23 Chapter 5 Installation If the filter wheel seems to be jumping in the slot you have inserted the wheel in a wrong way and the magnet is rejecting it Turn the wheel over
76. ndard s and samples This is achieved by using the following standardization and calibration procedures 1 Determinations having standard solution s in assay series end point two point standard mode and two point kinetic methods If one of the reagents contains protein or detergent the requirements of identical surfaces in all analyses cuvettes are met If sample serum is the only protein containing component in the assay standard solution s and reagents being aqueous the surface tension must be lowered by adding detergent into the reagent or by using protein containing standard and blank solutions 2 Determination based on the use of molar absorptivity two point factor mode kinetic methods In kinetic methods i e in reaction rate measurements the altered slope of substrate absorbance dependence curve becomes critical Therefore it is necessary to use the corrected molar absorptivity coefficient to obtain accurate results In most cases the corrected value of molar absorptivity is about 90 of the literature value e g 340 nm yap 6 22 and E 5 66 The corrected values of molar absorptivities are provided in the Multiskan EX applications 4 2 Program modules The instrument provides the following functions initiated through the keyboard or computer interface e Absorbance measurement e Shaking e Data transfer via the parallel port Sends measurement data and status information to an external p
77. ng in the event of contamination Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 42 Note E Warning Caution Caution Caution Caution Caution Note bb bl Fig 6 1 Chapter 6 Operation The printer destination is set to external printer as default If no printer is connected to the instrument the message NO CENTRONICS PRINTER is displayed Follow the instructions presented in Section 6 8 1 to set the printer destination according to the instrument configuration If the instrument is used in a manner not specified by the manufacturer the protection provided by the instrument may be impaired See Section 9 1 General specifications Do not use the internal printer without paper Make sure the paper will not roll between the paper roll and the instrument Help the paper to pass over the paper roll with your finger if necessary Do not smoke eat or drink while using the Multiskan EX Wash your hands thoroughly after handling test plates Observe normal laboratory procedures for handling potentially dangerous plates Use proper protective clothing Use disposable gloves Ensure that the working area is well ventilated Never spill fluids in or on the equipment Prevent any liquid from entering the instrument Immediately wipe away spilled liquids from outer surfaces to prevent damage and wipe with deionized distilled aqua Keep the instrument free of dust and other foreign mat
78. nk column 1 12 e The average of the blank values 8 of the column is used 4 COLUMN BLANK e Enter the blank column 1 12 e Eachrow will have an individual blank value that is used 5 ROW MEAN BLANK e Enter the blank row A H e The average of the blank values 12 of the row is used 6 ROW BLANK e Enter the blank row A H Each column will have an individual blank value that is used Thermo Scientific Multiskan EX User Manual Continued Rev 3 3 Cat no 1507300 53 Chapter 6 Operation Table 6 4 Measurement parameters Measurement parameter Function 1 BL ON EVERY PL 2 RETAINED BLANK You can also select either blanking on every plate 1 BL ON EVERY PL or retained blank 2 RETAINED BLANK blank value of a former plate is used If you select RETAINED BLANK 1 USE OLD BLANK 2 NEW BLANK NOW will be requested before reading the next plate 1 END RESULTS 2 ALL RESULTS In end point kinetic or dual wavelength measurements you can select final results e g absorbance differences and intermediate results e g absorbances with filter 1 and filter 2 INTERVAL TIME You can select the INTERVAL TIME parameter in two point and kinetic measurements It is the time between successive measurements of the plate The minimum interval time is 5 s the maximum 17 h 59 min 59 s with Primary EIA and 100 s with Cutoff and Cubic spline program modules Enter the interval t
79. nn 20 Environmental requirements cococconononoccconnnnnnnnnnnnnncnnnnnnnn naar nn nc cnn nn rra nana mr nr nn rre 20 Things to avoid ein a eden Ord ad id 20 Technical prerequisites iinic a nn cnn nn nene 20 Instrument layout and installation ooonnnnnnninncinnnnnnnnnanoccrcccnn narrar nn 21 Installing the filter Whe eles crecieron tere eee en ere en eter 23 Installing the lampia aaa a aaa e 24 Adjusting the plate carrier for different microplates eee 26 Mals SUpply Cable iii a sep Ee ae 27 Connecting to a computer or a printer enne nnns 28 Interface hardware specifications for the RS 232C interface ssss 28 Bauid rate selection t rete A 29 Handshake selection esses nennen nnne nnns nnne 30 Transmit receive pin configuration essen nnns 31 Parallel Centronics type printer interface 31 Signals connected to the DB 25 connector essseeseeeeeeneen enn 32 InterfaceconrnectloDS tica te Rene one rg E sere er Ren ad 33 Computer Cortrol dente Nach coetu etor ten eye ter eer ree ma errore e e E d rx 34 Computer control commands essesseesseeeeeeeeee nennen nennen nennen nnn nnns 34 Acknowledgement messages in Primary EIA program module 36 Availability of computer control commands esee nnns 37 Operational Gh 6k eet it egeo en dep eruta nae atte eas 38 Operations sc
80. ns input socket Fig 5 9 item 2 at the back of the instrument 3 Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded Mains switch Mains input socket Fuses Parallel port LPT for an external printer RS 232C interface connector for a computer Serial RS 232C DIL switches Secondary interface DIL switches NOGORWNS gt Fig 5 9 Multiskan EX rear view Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 27 Chapter 5 Installation 5 4 Connecting to a computer or a printer Data transfer to a computer occurs v a a built in serial RS 232C interface Data transfer to a printer may occur via a built in RS 232C interface or via a parallel Centronics interface 5 4 1 Interface hardware specifications for the RS 232C interface The hardware specifications for the interface are as follows 1 2 The configuration is specified as for data terminal equipment DTE You can select the baud rate i e the speed at which data is changed between an external computer and the instrument through the DIL switches at the rear of the instrument see Section 5 4 2 The following baud rates are available 150 300 600 1200 2400 4800 9600 and 19200 The baud rate 9600 is set by the manufacturer The character format is 10 bits with 1 start bit 8 data bits 1 stop bit and no parity The format cannot be changed It is recommended to use the DTR D
81. nter start stop mecs mode cok mode y Fig 4 5 Multiskan EX keyboard Table 4 2 Keyboard keys Key Function 0 9 To enter numeric values and microplate positions or operands for A H cutoff equations In equations first press the shift key then the letter P N L Symbols for positive P negative N or low positive L controls to be used in cutoff equations Press first the shift key Decimal point C To correct data before entering it print send To print or send out absorbances or calculation results via the selected abs interface to the selected printer See Sections 6 4 and 6 8 1 paper feed Feeds paper from the roll of an internal or external printer X Operators for cutoff equations Press first the shift key to enter and shift To take symbols at the upper right corner of the key into use enter To enter modes and parameters start To start the measurement stop To eject the microplate The instrument returns to the previous logical state save To store user determined programs in the memory of the instrument recall To recall programs stored earlier meas mode To select the measurement mode meas param To enter the measurement parameters calc mode To select the calculation mode calc param To enter the calculation parameters param To select the general parameters e g RS format and plate movement Arrow up to scroll backwards in the list of modes or parameters and WN to change the clock display Parenthesis open bracket used in
82. ntific Multiskan EX User Manual Rev 3 3 Cat no 1507300 100 Chapter 12 Index K Keyboard tal aia titi 17 Kinetic measurement Mod Osn araea aeaa aaa aaea a aa aaa aa ia aaa Ea aaa Eaa aa a aT a a A aeei 51 L Lag teme iiit A ROO Re ROTARUM T A A AEAT 52 Lambert Beeris Witt A cepe ru dee oce eerie 12 Latii unb iM o 22 changing zaran eti Pea feces dida do cedet ds 81 install iaa 24 Lenses aperture dnd coridenser ici Aa it 11 decontamination see a a E edo a e a dd ent TI eee daa 83 A NO 11 12 UPPER ia A AA 11 Eight SOULGCG iino o id sU Pd hdd RI 12 Limit calculation dii teen erui eee eti mde mtd tese us 53 Lin ar stand ard tb RSV rs 53 Liquid crystal display LCD circuit board ccconoccccnaninnncnnanoncnnnanoncnnnnnonnnnnnnr non nn naar nr nr nan rn r rar nnne 22 Eiquid suffaee ien ER e ERR EORR REV E ERU Edd daa dd 13 SI APC pete E 15 Long term stability i 90 LPT 1 interface CODFector at cinere eer e eee een ineo e a eere ies eene eee eva eu 27 M Magnet in the filter wheelslOt terc ctet ee detta etae beet ee o ea Leon e cere d Ie eta eCard ca dcl 24 80 on the Tilter WHEEL MEDIE 79 Mains power supply 5 6 testes RR as 90 Mains SWItCHi 2 nidad eia tread eT nus lads qud Da Aa CER Uwe nda s FUE Ca da vu dera ea ONT CR FR Raw Ug Ua 27 46 Maintenance eR De UD hit gei nee A RM 74 Oe Ea I 74 Measurement assembly COVE all ia 21 Measurement assembly cover conmicccccnnncncnonannnnnnnnn cnn 76 Measurem
83. o do upon delivery 5 2 1 How to unpack Move the unpacked instrument to its site of operation Unpack the Multiskan EX instrument and accessories carefully with the arrows on the transport package pointing upwards Refer to the enclosed packing instructions The following notes and instructions are sent with the instrument and are immediately available when you open the package e the Warranty Certificate card e the packing instructions packing list e the User Manual e the Transportation discrepancy report e the Linearity test printout Caution DO NOT touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty To lift the instrument put your fingers under the bottom on both sides and lift it with your back straight The net weight of the instrument is 11 kg 24 Ibs Caution When unpacking the instrument it is recommended that two people lift the instrument together taking proper precautions to avoid injury Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use If the optional printer has been ordered it is included uninstalled See Fig 5 1 Please con
84. oe pr perd ne Dome dtu oe cR ette Ree dei 55 example 2 nn mU PIIRmIDUD IEEE Dm e 65 D Data Signal 2 ia 32 Data transfer Unsuccess tul sro nia eire sft cies HX EE Ete E VER Hewes H T A ER 86 Date aNd E TI EUM 58 59 DB 25 COMME Ciria 32 Deconta Minato Mrs ad da 83 Certifica Oi A A A S A A A 105 INSUMOS A AAA At A EAE 83 POCO UE A does A cA A II IG ME 83 Detector frae a aaaea a aaa aa TNRERUORDC 76 78 BITTER 11 12 DIMENSIONS ia eet endete hetero ie nare or op p eerte deo ee slab ces po a e re ade ioa e Ier or eap dec ae y Ee bua 90 Directives ies ede peu it Dee EET TN D 91 Disinfectants eeen IDOL TEE DD ER RE 83 Disinfection procedure iei ee ce d ee ER n aU l rra EORR na 46 Display oneste td cd 21 CharacteriStiCS ERE 90 Disposal ISTMO a 85 BENE SESSEL id E 82 Double llmit rcd roa o cvs ed cere eese Sa ce a a de rc ERE teense deb da Lote Peer doa aa ERR 54 55 Dual wavelength measurement mode ccc cece eect ee ee ee ee eee nn nn nr rn nene 51 E Earththng RnB AS 27 End results oea ara ld 52 Environmental CONdItiONS cccccccccccnnnnnnnnnnnnonononononononononono nono nn E ad iaaa aiaa ai nn nn nan nnnnnnnnona 90 Equation errome iaa na DEL 87 Error Messages iii EQUO RM A E a dasha a a ES 86 Examples of programming Cubic spline program module seseseeeeeeeeee ener EAA AEAEE ARA SRNA RS ARENAEN 69 Cutoff program MOGUIG onpi a a dad E S 65 primary EIA program modul 2 etti
85. on Switch position Baud rate 3 2 1 down down down 150 up down down 300 down up down 600 up up down 1200 down down up 2400 up down up 4800 down up up 9600 set by the manufacturer up up up 19200 To determine which baud rate is necessary for any given computer printer 1 Consult the operating manual supplied with the computer or printer 2 Contact the dealer from which the computer or printer was purchased 3 Contact the computer or printer manufacturer Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 29 Chapter 5 Installation 5 4 3 Handshake selection DTR DSR handshake The DTR DSR hardware handshake makes it possible for two devices connected via the RS 232C line to check readiness before exchanging data or commands DSR Data Set Ready pin 6 When the DSR handshake is used the high positive state indicates that the printer or computer is ready to accept a data character The low negative state indicates that the printer or computer is busy and not ready to accept a data character Thus before sending each character the Multiskan EX waits until the other device sets the DSR line to the high state The DSR handshake is switched ON or OFF through DIL switch 4 Fig 5 12 o EN E M1 fun PB 87654321 876 4 32 1 Fig 5 12 DIL switch position 4 for handshake selection Table 5 3 DIL switch position for handshake Switch 4 position Handshake up enable
86. on 7 9 Change the lamp when blown See Section 7 5 e Change the fuses when blown See Section 7 6 pe Decontaminate the instrument if any biohazardous a A material has spilled or when shipping to service See Section 7 8 Perform verification with the Multiskan verification e plate Cat no 24072800 the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time or use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as damage may occur Caution Painted surfaces can be cleaned with most laboratory detergents Dilute Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 76 7 1 3 General A Chapter 7 Maintenance Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied Always ensure that the electrical supply in the laboratory conforms to that specified on the voltage label on the instrument To guarantee the continuous reliability and accuracy of the Multiskan EX avoid disturbing any of the optical system components A misalignment of the light path affects measurements e Keep the optical system clean to ensure proper functioning and accurate results e Prevent any liquid from entering the instrument e Keep the instrument free of dust an
87. r Manual Rev 3 3 Cat no 1507300 84 Chapter 7 Maintenance 7 6 Changing the fuses The fuses are located at the rear of the instrument Fig 5 9 item 3 1 Switch the power OFF 2 Unplug the instrument and disconnect the power cable from the power input socket 3 Unscrew the caps of the fuse holders Fig 7 8 Use a flat screwdriver and turn about 45 to the left The fuse cap is released and you can remove it Fig 7 8 Changing the fuses 4 Replace the blown fuses with the same type Replace always both fuses as they are usually both damaged 5 Screw the caps back onto the fuse holders Turn the cap until you can feel it going further in Turn it about 45 to the right with the screwdriver 6 Reconnect the power cable and switch the power ON 7 7 Disposal of materials When disposing of used material follow the Good Laboratory Practices GLP Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used A microplates microstrips disposable gloves syringes disposable tips etc as biohazardous waste Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 85 Chapter 7 Maintenance 7 8 Decontamination procedure A A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed A decontamination p
88. r open to control options on the circuit board Double limit Pertaining to cutoff equations The measured absorbance values are compared with two limit values defined by the user ElA Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A diagnostic test method to measure or detect a substance using antibody antigen reactions EN European Norm EU European Union Filter An optically wavelength selective component IEC International Electrotechnical Commission Kinetic Measurement over a time period at certain intervals measurement Microplate A rigid or framed polystyrene plate with microwells in different well formats e g 6 12 24 48 96 384 864 etc wells for ease of use in performing multiple tests through techniques such as EIA or ELISA Photometer A device for measuring the intensity of infrared ultraviolet or visible light Photometry Volumetric analysis in which the endpoint of a reaction is determined from color Thermo Scientific Multiskan EX User Manual changes detected by photoelectric means Rev 3 3 Cat no 1507300 105 Chapter 13 Glossary and Abbreviations Point to point Curve fit type These fitting types will pass through each of the points Adjacent response concentration coordinates are linked with straight lines linear interpolation Reagent A substance or solution used to produce a character
89. rement the plate carrier returns to the original position No error messages should appear on the display 1 PRIMARY EIA READY 12 30 If the instrument does not function properly contact Thermo Fisher Scientific s service department no printer is connected to the instrument the message NO CENTRONICS PRINTER is displayed Follow the instructions presented in Section 6 8 1 to set the printer destination according to the instrument configuration Note The printer destination is set to external printer as default If If you want to perform a verification of the instrument performance a Thermo Scientific Multiskan Verification plate Cat no 24072800 is available Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 38 Chapter 6 Operation 6 1 Operation check list The section on operation will contain an outline of the points mentioned in the check list below Table 6 1 Operation check list Tick Item Make a program Uu Program the measurement parameters Uu Program the calculation parameters Uu Save the program Measure a program Uu Recall the program Uu Start the measurement Print the results Uu Shut down the instrument The following symbols are used in the flowcharts below Press the key You can scroll the list of programming parameters forwards and backwards with the arrow keys You can also use the number keys to directly select the desired parameter DISPLAY TEXT Shows the text on
90. rinter e Data transfer via the serial port Receives commands from the external computer Sends data acknowledgements and status including appropriate error messages The Multiskan EX in built software comprises program modules which are combinations of different measurement and calculation modes See Table 4 1 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 15 Chapter 4 Functional Description Table 4 1 Measurement and calculation modes available in different program modules Measurement modes Primary Cutoff Cubic EIA spline absorbance e e e dual wavelength e e e two point e kinetic e multiwavelength computer control e Calculation modes Primary Cutoff Cubic EIA spline factor e linear standard e standard line limit m double limit range e column subtraction a point to point calculation e cutoff e cubic spline e Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 16 4 3 Keyboard The keyboard of the Multiskan EX comprises 30 keys with click action switches The keys available are presented in Table 4 2 E dE on Thermo Scientific Multiskan EX User Manual Chapter 4 Functional Description 5t 28 d print abs gP o paper feed A save recall porom 6 zi n meos parom col porom 4 3 C e
91. rinter interface Switch position 3 2 1 down up down Centronics type printer interface in use recommended down up up The results are sent simultaneously via the Centronics and RS 232C interfaces Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 31 Chapter 5 Installation 5 4 6 Signals connected to the DB 25 connector The following signals are connected to the DB 25 connector at the rear of the instrument Fig 5 9 item 5 Table 5 6 Data signals connected to the DB 25 connector RS 232C port DB 25 connector Pin no Signal name 2 TxD or RxD 3 RxD or TxD 6 DSR 7 GND 20 DTR Signal description 2 TxD Transmit Data output or RxD Receive Data input 3 RxD Receive Data input or TxD Transmit Data output 6 DSR Data Set Ready The input tells the Multiskan EX that the printer or computer is ready 7 GND signal ground 20 DTR Data Terminal Ready The DTR is in the high state when the Multiskan EX is ready to receive a command Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 32 Chapter 5 Installation 5 4 7 Interface connections Multiskan EX ES CHASSIS GN 1 CHASSISGNI Spe RxD 2 E TD AN xD E NE RxD nubere RE ES mE 5 5 DSR 6 ME D SIGNALG ND SGNALQND DCD 8 it DdD DIR 20 20 D Standard D25 Cable M M Standard Multiskan Ex Equipmen CHASSIS GND CHASSIS GNE 8765 TD 2 TxD
92. rocedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is any risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended that the complete decontamination procedure is performed before relocating the instrument from one laboratory to another Refer to Section 7 8 Decontamination is not required for the proper functioning of the instrument Example of decontaminants e Ethanol 7096 e Virkon solution 1 396 e Glutaraldehyde solution 4 e Chloramine T e Microcide SO 1 64 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde affect the enzyme being used in EIA tests in a negative way resulting in bad test results Warning Always use disposable gloves and protective clothing and operate in a well ventilated area 7 8 1 How to decontaminate the instrument A Decontaminate the instrument in case of a biohazard of infectious agents as follows 1 Turn the power OFF and unplug the instrument 2 Use disposable gloves 3 Clean the instrument outside the track Fig 7 1 item 4 and the plate carrier Fig 7 1 item 5 with a disposable cloth dampened either with 7096 ethanol or 196 glutaraldehyde 4 Remove the two 2 scr
93. roplates still on the plate carrier Dispose of all microplates and strips as biohazardous waste Switch the Multiskan EX off by pressing the mains switch Fig 5 9 item 1 into the OFF position Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled aqua a mild detergent SDS sodium dodecyl sulfate or soap solution If you have spilt infectious agents on the instrument disinfect with 7096 alcohol or some other disinfectant see the Multiskan EX User Manual Section 7 8 Last of all put the dust cover on 6 7 Emergency situations In case there is any abnormal situation during the operation such as fluids spilling inside the instrument follow these steps 1 2 3 Switch OFF the instrument Unplug the instrument from the power supply Perform appropriate corrective actions However do not touch the interior of the instrument Contact authorized service or your local Thermo Fisher Scientific representative Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 46 Chapter 6 Operation 6 8 Changing general parameters 6 8 1 Selecting the printing destination 4 PRINTER ON OFF 2 PRINTER ON READY Internal printer selected 1 RS FORMAT 1 PRINTER OFF 2 COMPUTER FORMAT READY Data transfer to a computer selected 1 PRINTER FORMAT 8 PRINTOUT SPACING 2 PRINTOUT SPACING READY External printer selected Thermo Scienti
94. s with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuse holders is prohibited covers or the removal of components is likely to expose live parts Disconnect the instrument from all voltage sources by disconnecting the mains supply cable before opening it for any adjustment replacement maintenance or repair Warning When the instrument is connected to the mains supply the opening of Any adjustment maintenance or repair of the opened instrument under voltage should be avoided if possible but if unavoidable should be carried out only by a skilled technician aware of the hazard The same precautions applicable when using any electrical equipment should naturally be observed with this instrument Warning DO NOT touch switches or electrical outlets with wet hands Switch the instrument OFF before disconnecting it from the mains supply 8 3 2 Defects and abnormal stresses electromagnetic perturbation which could impair the operation of other Warning If the instrument is not functioning properly it may create devices or equipment in the usual environment Whenever it is likely that the protection has been impaired the instrument should be made inoperative and be secured against any unintended operation Contact authorized technical service immediately The protection is likely to be impaired if for example the instrument 1
95. sitioned into its slot Fig 7 3 item 4 6 Replace the detector frame fasteners with washers Fig 7 2 item 1 Make sure that the detector frame fasteners are placed so that the edge in the fastener faces up Fig 7 2 e Caution DO NOT bend the measurement circuit board cable Fig 7 1 item 6 7 Lower the cover slightly to the left of its position Make sure that all sides are firmly pressing against the instrument frame and slide the cover tightly to its place 8 Replace the two cover retaining screws with their plastic washers The screw Fig 5 2 item 7 will connect the measurement assembly cover to the instrument ground to suppress electrical disturbances 9 Clean the interference filters with a napless cloth or with a lens paper It is recommended not to use liquids when cleaning the filters Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 79 Chapter 7 Maintenance 1 Detector frame fasteners with washers Fig 7 2 Opening the detector frame Handle Upper lenses Hall element sensor Position of the Hall element sensor Pon gt Fig 7 3 Lifting the detector frame Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 80 Chapter 7 Maintenance 7 4 Changing individual filters in the filter wheel Caution Only use filters approved by the supplier 1 Switch the power OFF 2 Unscrew the two 2 cover retaining screws Fig 5 2 item 6 and lift
96. sssests 39 Operation check Totes reed een rt Oi etd cd 39 Making a programi miccional ei dido ed 40 MG aSUning ee E 42 lisse data vicio 44 Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 4 6 5 6 6 6 7 6 8 6 8 1 6 8 2 6 8 3 6 8 4 6 9 6 9 1 6 9 2 6 9 2 1 6 9 2 2 6 9 2 3 6 9 3 6 10 6 10 1 6 10 2 6 10 3 7 1 7 1 1 7 1 2 7 1 3 7 1 4 7 1 5 7 2 7 3 7 4 7 5 7 6 7 7 7 8 7 8 1 7 9 7 10 7 11 8 1 8 2 8 3 8 3 1 8 3 2 9 1 9 2 9 3 Table of Contents Printing stored programs oie eut eds 45 Shutdown proced re ciini tain nto ter nece ae oda adeat 46 Emergency SIt gtlOns td 46 Changing general parameters essssessseeeen eene nnn nnne nnns 47 Selecting the printing destination oonnccccnnnoccccnanonnncnnnnnnnnnnnno cnn nnnnn nnne 47 Selecting the plate movement esssessesseee esee nennen enne nnne nnn enne 48 Selecting shaking erecti aia 49 Entering filter wheel parameters oooonoccccconccccncnononnnnnanonnncnnnnnnncnnnn a nn nc rana n nr ran nn nrnnnnss 50 Programming parameters of the Multiskan EX essen 52 Measurement modes and parameters ccccccccnnonononccincnnnannnoncnncnnnnnnnnnn nc nn rn rra nn 53 Calculation parameters essen nnne rre nnne rra 55 Primary EIA program id ee neni er to ERE rias 55 Cutoff OO GT arm eic dd er Ra a dai EFE
97. tact technical service for installation Paper roll shaft Fig 5 1 Optional printer as delivered all parts not visible 5 2 2 Checking delivery for completeness Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 19 Chapter 5 Installation 5 2 3 Checking for damage during transport Visually inspect the transport package the instrument and the accessories for any possible transport damage If the transport package has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy 5 2 4 Environmental requirements When you set up your Multiskan EX avoid sites of operation with excess dust vibrations strong magnetic fields dir
98. ter 2305300 RS 232C PC serial cable F M 2303850 RS 232C interface cable for MAC 24071230 Filter wheel without filters 142xxx0 Other filters in the 400 750 nm range can be ordered xxx corresponds to the wavelength 1041980 5185430CD Filter box Ascent Software v 2 6 for Multiskan 1507300 User manual N02724 Brief User s Guide 1610002 Dust cover 10 2 Spares for maintenance Code Item 2400620 Lamp quartz tungsten halogen lamp Osram 64607A 8V 50W 1600510 Thermal paper MTP201 58mm 1210950 Fuse 3 5 A 10 pcs box 10 3 Verification plates Code Item 24072800 Multiskan Verification Plate 24073500 Multiskan Verification Plate includes Ascent Software Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 96 Chapter 11 Warranty Certificate 11 Warranty Certificate Thermo Fisher Scientific Microplate Instrumentation Business Multiskan EX is fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of three 3 years from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific s instructions The warranty is invalid if products
99. ter Clean and keep dry the plate carrier and transfer rails to prevent jamming You can print the programs stored in the instrument memory by pressing the param key and selecting 3 PROGRAM PRINTING TITAN ACERCAR Positioning the microplate correctly Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 43 Chapter 6 Operation 6 4 Printing data The calculated results are automatically printed after the measurement The printout is sent to the internal printer the external printer or the computer according to the general settings of the instruments Section 6 8 1 If you want to print the raw measurement data o Note You can only print the results directly after the measurement After you perform any other actions such as saving the program the measurement and calculation data are no longer in the instrument memory Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 44 Chapter 6 Operation 6 5 Printing stored programs Select the program module from which you want print the program parameters You can print a range of programs but the parameters are printed only for the programs in the selected module f 1 PRIMARY EIA i 2 CUTOFF READY 3 CUBIC SPLINE Heca PROGRAM PRINTING START PRGM NO 1 STOP PRGM NO 64 READY Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 45 Chapter 6 Operation 6 6 Shutdown procedure Q Warning Remove any mic
100. tes are measured The scale used is tried with log lin and lin lin Use of an external printer for recording the results 1 2 3 4 5 6 7 8 9 10 11 12 A Blank Un2 B Std 1 Un 3 C Std 2 etc D Std 3 E Std 4 F Std 5 G Std 6 H Un 1 Fig 6 4 Plate layout in the example for Cubic spline program module Switch the instrument on Wait one 1 minute Select the program module 3 CUBIC SPLINE READY Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 71 Chapter 6 Operation Select the meas measurement mode mode 1 ABSORBANCE MODE Select the meas measurement param parameters FILTER 340 Enter the wavelength of the filter or the filter position number 1 NO REAGENT BLANK 2 SINGLE WELL BLANK Position of the blank BLANK WELL A1 Blanking possibilities 2 RETAINED BLANK READY Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 72 Chapter 6 Operation calc mode 2 CUBIC SPLINE calc param Number of standards NR OF STDS six in this example Number of replicates NR OF REPL one in this example Concentration of the standard STANDARD 1 0 000 six in this example NR OF STDS Location of the replicate STD 1 REPL 1 iesus date nut only one in this example 1 STD ON EVERY PLATE Select retained standard The standard curve of the former plate is used
101. the cover Caution When handling the filter wheel do not touch any other mechanical or electronic part 3 Lift the filter wheel from the filter wheel slot Do not touch the filter surfaces Fig 7 4 Removing the filter spring 4 Remove the filter spring by unscrewing the four 4 spring position holding screws Caution Do not touch the filter glass surfaces with your bare fingers Caution The magnet in the middle of the filter wheel attracts both the screwdriver and the screws Make sure the screwdriver or the screws do not scratch the filters Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 81 Chapter 7 Maintenance Spring position holding screws Fig 7 5 Filter wheel parts Fig 7 6 Individual filter showing the arrow 5 Insert a new filter or an additional optional filter into the filter wheel when the filter wheel is in the position shown in Fig 7 5 so that the arrow on the filter rim points downwards in the direction of the light Fig 7 6 The wavelength of the filter is marked on the side i e the three digits before the last digit of the number sequence xxxxx NNNx where NNN is the wavelength The filter positions are marked on the other side of the filter wheel The filter wheel has at present three filters factory installed Table 7 2 Note Do not change the positions of the factory or earlier installed filters Insert the optional filters into filter wheel positions a
102. the connection cable The printer is not printing The printer has no paper A wrong cable is in use The printer cable is connected to the RS 232 port The printer settings are incorrect Add paper Check the used cable Connect the printer cable to the printer port Fig 5 9 item 4 Check the printer settings Section 6 8 1 Check the DIL switches Section 5 4 The printer prints overlapping rows The printer paper in the printer is jammed Ensure fluent flow of the printer paper Internal printer stops printing The printer paper in the internal printer is jammed Ensure fluent flow of the printer paper Contact authorized technical service Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 90 Chapter 8 Troubleshooting Error Cause Action PLATE ERROR FILTER ERROR The filter wheel is missing or faulty Check that the plate is properly positioned in the plate carrier Fig 6 1 Check that no foreign matter is obstructing the plate carrier Check that the rails are clean and not bent Check that the toothed belt is not broken Place the filter wheel into the filter wheel slot Check that the filter wheel has been properly installed or is not broken Check the condition of the filter wheel position slots Fig 5 4 TOO MUCH LIGHT Too much light enters the detectors The filter wheel may be missing or
103. the interface 2 DOUBLE LIMIT The measured absorbance values are compared to two limit values defined by the user If the result is lower than the lower limit a negative sign is sent If the result is between the two limit values 0 is sent If the result is higher than the upper limit a positive sign is sent The measurement parameters calculation parameters and the results in a 0 matrix are sent via the interface Parameters Low limit value LOW LIMIT 0 000 2 000 High limit value HIGH LIMIT 0 001 2 000 Continued Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 57 Table 6 6 Calculation parameters in Cutoff program Chapter 6 Operation Cutoff program calculation parameter Operation 3 RANGE CALCULATION Parameters 4 CUTOFF Parameters CUTOFF EQUATION NO OF POS CONTROL POS CONTROL A1 NO OF NEG CONTROL NEG CONTROL A1 NO OF LOW POS CONTROL LOW POS CONTROL A1 GREY AREA INTERPRETATION 1 gt 2 gt The range value defined by the user is divided into ten 10 equal segments The segments are numbered from 0 to 9 The measured absorbance value is represented by one of these numbers depending on which segment it lies in The measurement parameters calculation parameters and the matrix where the results are shown by segment numbers 0 9 are sent via the interface Range value RANGE 0 000 2 000 Example if an absorbance range
104. tion Table 5 7 Computer control commands Command Function I Initializing the starting column so that the absolute starting column is 1 Example l LF S2 LF M4 LF columns 3 4 5 and 6 are measured 0 Driving the plate out and initializing the starting column so that the absolute starting column is j Example O LF S2 LF M4 LF columns 2 3 4 and 5 are measured X1 Set the shaking speed to maximum This speed is selected as default X2 Set the shaking speed to 66 of the maximum X3 Set the shaking speed to 33 of the maximum Zn Shaking of the microplate n is the shaking time in seconds n 00 60 Example a shake of 5 s is entered as Z05 Date and time Serial number Version number of the software o zd Returning to keyboard use The commands T N and V can also be used while the instrument displays READY Each command must be terminated by LF line feed The Multiskan EX disregards spaces before the command Up to 48 extra characters between the command and LF will be ignored Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 35 Chapter 5 Installation 5 5 2 Acknowledgement messages in Primary EIA program module The Multiskan EX acknowledges each command by sending a message The following messages are used OK The action has been successfully performed ER1 Error see Section8 Troubleshooting ER3 An invalid command t
105. u like to receive information about other Thermo Fisher Scientific products Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 110 Appendix D Addresses For the latest information on products and services visit our worldwide web sites on the Internet at http www thermo com Manufactured by Distributed by Thermo Fisher Scientific Oy P O Box 100 FI 01621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 111 Notes Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 112 Notes Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 113 Notes Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 114
106. ubject to the RoHS Directive are available at www thermo com WEEEROHS Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 88 Chapter 7 Maintenance The instrument includes a lithium battery Follow the local regulations for the disposal of batteries Regarding the original packaging and packing materials use the recycling operators known to you For further information contact your local Thermo Fisher Scientific representative Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 89 Chapter 8 Troubleshooting 8 Troubleshooting A 8 1 Troubleshooting guide DO NOT use the instrument if it appears that it does not function properly Warning The problems covered below are considered as faults that require repair or corrective repair If the installation procedure is carefully followed no faults should arise However if problems occur or reoccur contact authorized technical service immediately Table 8 1 Troubleshooting guide and error messages Error Cause Action No power when switched on Check that the instrument is plugged in Check that the fuses are not blown Unsuccessful transfer of data via the interface system Check that both the instrument and the computer have the same baud rate character length and handshake settings See Section 5 4 Check that the transmit receive pin configuration is correctly set See Section 5 4 Check
107. ugh the bottom of the wells through the samples via the upper lenses to the detectors which measure the intensity of light 4 1 1 Vertical measurement principle In vertical photometry the light beam enters the cuvette through its bottom i e optic window and then passes through the surface of the solution to a detector It follows from this optical arrangement that the absorbance depends on the amount of absorbing substance in the cuvette and not on the concentration explained below According to the Lambert Beer s law A exl c where A absorbance Error e molar absorbtivity cm mol I path length cm c concentration mol l Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 12 Chapter 4 Functional Description When we have n V C and l V where n amount of the absorbing substance mmol V volume of the solution in the well ml a cross sectional area of the well cm By substituting c and l in the Lambert Beer s law we will get Ape s uf a V a and a n A When a and e are constants the sample concentration can be calculated directly from the absorbance A when the molar absorbtivity e in cm mol I and cross sectional area of the well are known 4 1 2 Surface of the liquid in a cuvette Since the free surface of the solution in the cuvette will form the second window through which the light passes the properties of this surface are
108. useful life of the lamp switch off the instrument when it is not in use A warm up period of one 1 minute has to be allowed before you measure Wait one 1 minute Select first the program module instrument selects the proper light intensity for each installed filter o o Take the stored program into use Check that the filter and the plate are correct and that the plate is placed properly on the plate carrier Fig 5 2 item 9 with the A1 well in the upper left hand corner Fig 6 1 Place the microplate onto the lat i z E It is recommended that the assay include internal quality controls MEASURING The instrument performs the measurement READY After the measurement the results are printed on the internal printer or sent via the interface and recorded on the external printer or the computer according to the selection see Section 6 8 1 Check from Remove the microplate the printout that the used general and measurement parameters were from he as intended plate carrier Caution Handle the microplate with care to avoid any contamination of the instrument and therefore subsequent specimens The contamination of the specimen leads to incorrect results If the contents of the microplate do spill however follow the decontamination procedure given in Section 7 8 Follow also the system supplier s instructions in regard to the external PC software reagent kit and specimen handli
109. uter control commands in different program modules Command Meaning MOD 1 MOD 2 MOD 3 Primary Cut Off Cubic EIA Spline R Start remote control e e e Fn Filter n 1 8 e e e FMnn Multifilter nn 12 pcs e 2 En Measurement mode n 0 1 e e C Cn Module change n 1 3 A Air blank e e B Column blank e e P Pn Plate measure n 1 99 e e m PM Multifilter measure e Mn Column measure n 1 12 e Si Set column j 1 12 e e O Initialize column 0 e e l Initialize column 1 e e Xn Mixing speed n 1 3 e e e Zn Mixing n 00 60 e e e T Date and time e e e N Serial number e e e V Software version e e e Q Quit remote control e e e Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 37 Chapter 5 Installation 5 6 Operational check Before you take the instrument into use make time to perform the following operational check 1 Connect the printer Refer to 6 8 1 If the printer is not in use check that the printer settings are set to off 2 Switch the instrument on using the power switch at the rear of the instrument Fig 5 9 item 1 When the instrument is switched on wait about one 1 minute for the instrument to warm up 3 Startthe preinstalled measurement program in the instrument memory Press 4 Check that the plate carrier moves under the measurement assembly cover Fig 5 2 items 9 and 2 that the instrument starts the measurement and that after measu
110. w and number keys The entered values are used until changed 7 CLOCK SET Date and time year month day day of the week hour and minute can be changed with the arrow and number keys 8 PRINTOUT On the printout of the external printer you can select SPACING whether to leave an empty row between the results of the rows printout spacing On Off selection of the printout spacing 1 NO SPACING 2 PRINTOUT SPACING 9 MIXING Mixing shaking of the microplate before measurement is started 1 MIXING OFF 2 MIXING ON e MIXING FAST e MIXING MEDIUM e MIXING SLOW MIXING TIME enter the time in seconds 0 60 s The default value is 15 s 10 MODULE A list of the program modules is printed out LISTING Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 60 Chapter 6 Operation Table 6 9 Default values of the general parameters Parameter Default value 1 RS FORMAT 1 PRINTER FORMAT 2 PLATE MOVEMENT 1 CONTINUOUS 5 s 4 PRINTER ON OFF ON 5 PLATE ANALYSIS 1 ANALYSIS OFF 6 FILTER WHEEL NR OF FILTERS 3 FILTER 1 WAVEL 405 Filter wavelengths in nm FILTER 2 WAVEL 450 FILTER 3 WAVEL 620 7 CLOCK SET Date and time are set at the factory 8 PRINTOUT SPACING 1 NO SPACING 9 MIXING 1 MIXING OFF MIXING TIME 15 seconds Thermo Scientific Multiskan EX User Manual Rev 3 3 Cat no 1507300 61 Chapter 6 Operation 6 10 Examp

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