NGFG10 User Manual
Contents
1. Emission Filter 520 nm Amber Filter Lighting Blue Green Light Peak at 505 nm Cyan LED Light Epi White Light LED White Light White Back Light LED White Light Panel Interface Display Build in 2 7 TFT LCD SE G P95D thermal printer and USB flash drive VGA out Resolution 800 x 600 and 1024 x 768 General Info Power AC Adaptor 12V 4 16A Dimension W x H x D 230 x 230 x 254mm 9 05 x 9 05 x 10 in Gel Tools Shovel Amber View Shield Gel Tray USB Stick Amber Google Weight 3 2Kg 7 05 Ib Certification CE FCC 4 SEN Binsfelder Stra e 77 ZS See 52351 D ren Z ss 52551 D ren Germany E 49242120846 iil 49 2421 20 84690 49 2421 20 84 69 1 Balnfo nippongenetics eu TT eS a SE art Genetic PPON Genetics a EUROPE 6 NOMENCLATURE Front Upper lid Power Button LCD display Up Down Left Right OK button USB 2 0 Port Cyan Light White Light selection switch Scan button Back DC power USB 2 0 port for thermal printer P95DE VGA Out Port l 5 amenes Binsfelder Stra e 77 52351 D ren Germany 49 2421 20 84 69 0 49 2421 20 84691 4 lnfo nippongenetics eu SR A NIPPON Ge M NIPPON Genetics EUROPE 7 INITIAL SETUP Remove all tapes that hold items in place for shipping II Place the FastGene Blue Green GelPic LED Box unit on a flat surface Ill Connect the included AC2. the filter on lid make sure the two bent areas are placed against the lid Alternatively use the amber goggle Ill Turn on and off the blue green LED by pressing Scan button IV Excise the DNA Filter shield hanging tab Amber Filter Shield 8 4 Binsfelder Stra e 77 a 52351 D ren Germany Il 49 2421 20 84690 49 2421 2084691 si lnfo nippongenetics eu A SP zz s t KS a eau NIPPON Genetics 10 SCANNING A COLORIMETRIC STAINED GEL I Open the lid of the FastGene Blue Green GelPic LED Box Il Remove the black velvet sheet from the inside of the lid lll Place the gel tray with the protein gel on to the view area and close the lid Note the view area is water proof Hence the usage of the gel tray is not obligatory IV Slide the Light Selection Switch on the front side of the scanner to select the white light source for stained gel scanning V Selecting Transmitted Mode a Press OK button to activate the menu Use left or right keys to select L icon Use up and down keys to select White Back Light Press the OK button to confirm A live image of the gel will be shown on the display 2295 Remark Image adjustment and scanning is only possible in the live view mode If the following described icons are not shown at the menu bar after pressing OK button please refer to section 14 A 2 to switch between playback mode and live view mode Image Adjustment Exposure
3. 5 to 5 IV Press the OK button to confirm the exposure value V Wait until the image exposure is adjusted Image Adjustment BW BW invert l Press the OK button to activate the menu ll Use left or right keys to select icon Ill Use up and down key to select the desired BW option IV Press the OK button to confirm your selection V Wait until the image is adjusted Image Recording Press SCAN button green LED will flash and Saving don t remove USB will be displayed on the screen The scanned image will automatically save to the USB flash drive Printing Yes No option will be displayed on the screen if a thermal printer is connected to the FastGene Blue Green GelPic LED Box Scanning of the gel sample is now complete J d Binsfelder Stra e 77 a 52351 D ren Germany El 49 24212084690 M 49 24212084691 fm Info nippongenetics eu Yaa YY ch A NIPPON Ge M NIPPON Genetics EUROPE 9 EXTRACTION OF DNA FROM AGAROSE GELS FastGene Blue Green GelPic LED Box is equipped with an amber filter shield and amber goggle to filter the blue green excitation light enabling the extraction of DNA from the agarose gel Open the lid to its maximum opening and the blue green light will automatically turn off Note Light bleaches the DNA dye The light is switched off to avoid excessive bleaching Il Position the central opening of the amber filter shield on the hanging tab After hanging
4. Genetics NIPPON Genetics EUROPE FastGene Blue Green GelPic LED Box Cat No GPO4LED SL Binsfelder Stra e 77 Frew 52351 D ren Germany E 49 2421 20 84 69 0 49 2421 2084691 Bilnfo nippongenetics eu m RE CONTENT 1 SAFETY INFORMATION seen nn 3 2 WARRANT ee ee erh 3 3 UWE POT 405167 H 3 4 IMPORT ANENOTIGE ne near 4 9 SPEGIFICA TION DE 4 6 NOME NG CA TO RE ee ee ee 5 L IN II TE E ae seven cuveuaavneacuieeauepbenasesaupere E 6 8 SCANNING AN AGAROSE GEI 7 g EXTRACTION OF DNA FROM AGAROSE GELS seeeeesnessesssrrrrrrrrrresrerrrnn 8 10 SCANNING A COLORIMETRIC STAINED GEL ssesssesssessennrresssrrrrrrssrrrrereeee 9 11 SCANNING WESTERN BLOT MEMBRANE PETRI DISHES 10 12 CONNECTING AN EXTERNAL MONITOR OR BROUIECTOR eee 11 13 CONNECTING A MITSUBISHI P95D THERMAL DRHINTER 12 14 CONTROL E EE 13 A1 MENU IIUVEMODEL 13 A2 MENU Il PLAYBACK MODE nennen ERR Ea en 14 15 CONTACT IDE TAN Si sisstrceas nassen arena naar innen 15 Genelies Binsfelder Stra e 77 52351 D ren Z ss 52351 D ren Germany 49 2421 2084690 ll 49 2421 20 84 690 FN 49 2421 2084691 j 4Info nippongenetics eu 07 Kl wer NIPPON Genetics EUROPE Be NN 0 1 SAFETY INFORMATION Please read carefully the following notes to be able to properly use the FastGene Blue Green GelPic LED Box Imaging System 4 Please wear the appropriate personal protective equipment Using F
5. adapter to the back of the unit then plug into an electrical outlet IV Push the power button V Insert the USB flash drive VI Initiate the desired application see sections 8 to 11 6 amenes Binsfelder Stra e 77 52351 D ren Z ss 52351 D ren Germany 49 2421 2084690 49 242120846 fl 49 2421 20 84690 11 49 2421 2084691 i Info nippongenetics eu 10 DS ite S a A 4 NIPPON Genetics E me TE eo EUROPE 8 SCANNING AN AGAROSE GEL Open the lid of the FastGene Blue Green GelPic LED Box Slide the black velvet sheet into the groove of the lid to cover the white light area of the lid See figure below ll Place the gel tray with the agarose gel on to the view area and close the lid Note the view area is water proof Hence the usage of the gel tray is not obligatory Ill Slide the Light Selection Switch on the front side of the instrument to choose the cyan light source IV A live image of the gel will be shown on the display Remark Image adjustment and scanning is only possible in the live view mode If the following described icons are not shown at the menu bar after pressing OK button please refer to section 14 A 2 to switch between playback mode and live view mode Image Adjustment Exposure l Press the OK button to activate the menu 3 ll Use left or right keys to select Icon Ill Use up and down key to select the desired exposure value
6. astGene Blue Green GelPic LED Box will normally not injure eyes skins and samples However prolonged exposure of human naked eyes with light irradiation in the blue spectrum may increase the probability of suffering from retina diseases Therefore we recommend wearing the amber goggle included in the box when handling the FastGene Blue Green GelPic LED Box with an open lid 2 WARRANTY The FastGene Blue Green GelPic LED Box is warranted against defects in materials and workmanship for 1 year In case of any defects occurring in the instrument or accessories during this warranty period Nippon Genetics Europe will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded e Defects caused by improper operation e Repair or modification done by anyone other than Nippon Genetics Europe or an authorized agent Damage caused by substituting alternative parts Use of fittings or spare parts supplied by anyone other than Nippon Genetics Europe Damage caused by accident or misuse Damage caused by disaster Corrosion caused by improper solvent or sample For any inquiry or request for repair service contact Nippon Genetics Europe or your local distributor Please send a message containing information about the model and serial number of your instrument 3 REGULATORY NOTICE IMPORTANT This Nippon Genetics Europe instrument is designed and certified to meet sa
7. fety standards and EMC regulations Certified products are safe to use when operated in accordance with the instruction manual This instrument must not be modified or altered in any way Alteration of this instrument will e Void the manufacturer s warranty e Void the safety and EMC certification e Create a potential safety hazard Nippon Genetics Europe is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended or by modifications of the instrument not performed by Nippon Genetics Europe or an authorized agent 3 SEN Binsfelder Stra e 77 ZS See 52351 D ren Germany fl 49 2421 20 84 690 49 2421 2084691 Wlnfo nippongenetics eu O0 OOO E eae Oo re 2 72 NIPPON AR ei EUROPE 4 IMPORTANT NOTICE Please read the installation instruction carefully before installing the FastGene Blue Green GelPic LED Box Imaging System This instrument is meant for use only by specialized personnel that know the health risks associated with blue green light radiation and with reagents that are normally used with this instrument Please wear the amber goggle for your own protection 5 SPECIFICATION Imaging Capturing Unit Sensor CMOS Sensor Resolution 9 Mega Pixels Exposure Time 0 2 2 sec 11 scales USB flash drive View Area 16 x 10 5 cm fixed lens Image Storage Lens and Filter Filter Turret Manual Driven 2 position
8. green LED will flash and Saving don t remove USB will be displayed on the screen The scanned image will automatically save to the USB flash drive Printing Yes No option will be displayed on the screen if the Mitsubishi P95D thermal printer is connected to the FastGene Blue Green GelPic LED Box Scanning of the sample is now complete Epi mode is to scan the western blot membranes or petri dishes The black velvet sheet reduces the background 10 fd Binsfelder Stra e 77 af 92351 Duren Germany El 49 2421 20 84 69 0 wllr49 2421 2084691 a Info nippongenetics eu RN EI f F Ze A MH NIPPON Genetics geng Genetics EUROPE 12 CONNECTING AN EXTERNAL MONITOR OR PROJECTOR Images from the scanner can be displayed on a monitor using the VGA out port Set the monitor to VGA mode see monitor documentation for details ll Connect the VGA cable not included to the video port of the monitor and of the FastGene Blue Green LED GelPic Box Ill Press the OK button to activate the menu IV Use left or right keys to select Di icon V Use up and down key to select the option Display and press the OK button to confirm VI Use up and down key to select the option VGA and press the OK button to confirm VII Wait until the image is shown on the external monitor and press the OK button within 10 seconds again to confirm the connection to the external monitor NIPPON Gene
9. l Press the OK button to activate the menu 0 ll Use left or right keys to select icon lll Use up and down key to select the desired exposure value 5 to 5 IV Press the OK button to confirm the exposure value V Wait until the image exposure is adjusted Image Adjustment Color option n ivate the menu Press the OK button to activate the menu Built in White light plate ll Use left or right keys to select icon lll Use up and down key to select the desired Color option IV Press the OK button to confirm your selection V Wait until the image exposure is adjusted Image Recording Press SCAN button green LED will flash and Saving don t remove USB will be displayed on the screen The scanned image will automatically save to the USB flash drive Printing Yes No option will be displayed on the screen if the Mitsubishi P95D thermal printer is connected to the FastGene Blue Green GelPic LED Box Scanning of the gel sample is now complete J d Binsfelder Stra e 77 52351 D ren Germany El 49 2421 20 84690 49 24212084691 Wlnfo nippongenetics eu NT Nu EE u KR A eau NIPPON Genetics Fa free EUROPE 11 SCANNING WESTERN BLOT MEMBRANE PETRI DISHES Open the lid of the FastGene Blue Green GelPic LED Box ll Slide the black velvet sheet into the groove of the lid to cover the white light area of the lid Ill Place the gel tray with
10. mode Use left or right keys to select the icon and use the up or down key to select a setting and press OK to activate it LCD Dark Normal Bright Il Display LCD VGA Ill Video Output 800 x 600 1024 x 768 IV Save Format TIF JPG BMP JPG is default setting V Setup File Name 6 characters combination VI Quick View 1 sec 2 sec 3 sec off 2 sec is default setting VII Slide Show Interval 2 sec 3 sec 5 sec 10 sec 3 sec is default setting VIII Power Save 1 min 3 min 5 min 10 min 1 min is default setting IX Language English Japanese X Date setup date yyyy mm dd Xl Time setup time hh mm ss XII Reset Yes No YES Resets all current settings back to factory default XIII About F W S W information Update 13 fd Binsfelder Stra e 77 S 52351 D ren Z ss 52351 D ren Germany E 4924212084690 El 49 2421 20 84 69 0 Im 49 2421 20 84691 lnfo nippongenetics eu a ge WE C tier TR d A3 Menu Il PLAYBACK MODE Press the Ok button to activate the menu D Playback mode icon Use left or right keys to select the icon and press OK to switch to live view mode Auto Playback Mode To automatically play the captured images one by one Use left or right keys to select the icon Press the OK button to start autoplayback Edit Mode To edit the captured images Use left or right keys to select the icon and use the up or down key select the options rotate fli
11. p or delete the captured image Press the OK button to confirm Preview Mode To preview the captured images in a multi image thumbnail panel 1x1 2x2 and 3 x 3 images Use left or right keys to select the icon and use the up or down key select the options Press the OK button to confirm Di Set Up Mode Same as the Set Up Mode functions in Menu live mode 14 Einsfelder Stra e 77 FA EUROPE S 52351 D ren Germany fl 49 2421 20 84690 49 2421 2084691 Klnfo nippongenetics eu 15 CONTACT DETAILS Please contact us for additional information info nippongenetics eu Please contact our support support nippongenetics eu NIPPON Genetics EUROPE GmbH Binsfelder Strasse 77 52351 Dueren Germany Fon 49 2421 2084690 Fax 49 2421 2084691 15 W Binsfelder Stra e 77 52351 D ren Germany 49 2421 2084690 49 2421 2084691 x4 Info nippongenetics eu
12. the membrane petri dish on to the view area and close the lid Note the view area is water proof Hence the usage of the gel tray is not obligatory IV Slide the Light Selection Switch on the front side of the scanner to select the white light source for stained gel scanning V Selecting Epi Light Mode a Press OK button to activate the menu Use left or right keys to select L icon Use up and down keys to select White Epi Press the OK button to confirm A live image of the gel will be shown on the display 2295 Remark Image adjustment and scanning is only possible in the live view mode If the following described icons are not shown at the menu bar after pressing OK button please refer to section 14 A 2 to switch between playback mode and live view mode Image Adjustment Exposure Press the OK button to activate the menu 2 ll Use left or right keys to select icon ll Use up and down key to select the desired exposure value 5 to 5 IV Press the OK button to confirm the exposure value V Wait until the image exposure is adjusted Image Adjustment Color option l Press the OK button to activate the menu Black velvet sheet ll Use left or right keys to select icon lll Use up and down key to select the desired Color option IV Press the OK button to confirm your selection V Wait until the image exposure is adjusted Image Recording Press SCAN button
13. tics EUROPE GmbH Gone 11 BEN Binsfelder Stra e 77 ZS See 52351 D ren Germany Tl 49 2421 20 84 69 0 FTI 49 2421 20 84 69 1 en Info nippongenetics eu IN welt ba EH Vaasa NIPPON Geneties d Genetic tics EUROPE 13 CONNECTING A MITSUBISHI P95D THERMAL PRINTER Connect the USB cable not included to the USB port at the back side of the FastGene Blue Green GelPic LED Box and to the Mitsubishi P95D Thermal Printer ll Scan an image and follow the instructions shown on the display 12 MAIN Binsfelder Stra e 77 EEE 52351 D ren Germany IN OR il 49 2421 20 84690 BU 49 2421 2084691 WEklnfo nippongenetics eu ee NIPPON AR EUROPE 14 CONTROL MENUS A1 Menu I LIVE MODE Press the OK button to activate the menu Ei Live mode Icon Use left or right keys to select the icon and press OK to switch to playback mode LED Lighting Mode Use left or right keys to select the icon and use the up or down key to select Transmitted or Epi light source Press the OK button to confirm Note this mode is available only when white light application is selected Exposure Mode Use left or right keys to select the icon and use the up or down key to adjust the exposure value in this mode Press the OK button to confirm B W or Color Switch Mode Use left or right keys to select the icon and use the up or down key to select a color option Press the OK button to confirm Di Setup
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