User`s Manual - Metertech Inc.
Contents
1. 7 The obtained raw data in above file can be opened in Excel for further processing and calculation 43 Set Proqan Access ard Def aks SIS tre Ze WR Windows Catala d Windows Unde Tomat Network Connections FQ Satup Weard Sj Connection wizard SE Rense Desktop Connection 2 Wireless Nebwoek Setup Merosot Word W MENE pie ai Epress p Ramata Aedctance Tour Windev XP ResPlaver EG windows Epos Adobe Photoshop Abun Starter Editon 30 BR windows Morte Maher wiriad QD Addase Book 8 Command Prompt Notepad H Part Frog an Conpsciblity Wizard OJ Synchronizes Figure B 1 New Conner onnection Beseriptian K Enter a name and choose an icon forthe correction Figure B 2 44 teat HyperTi Connect To COMZ Properties Figure B 3 Figure B 4 45 HyperTerminal Send Text Capture to Printer Figure B 5 test HyperTerminal Capture lest Figure B 6 462. Move the cursor one place to the right Move the cursor one place up or input 26 English letters forward Move the cursor one place down or input 26 English letters backward For entering numerical data or hot key protocol For entering well name of microplate Move plate carrier out of or into machine while in main screen Move back to previous option previous question last window Move to next option next question or next window Confirm selections or entries of fields or parameters Delete current number or letter at the cursor position Undo current action or delete current entries Initiate plate reading with selected protocol Terminate reading process or stop printing process or cease protocol editing process or leave warning message 120 3 Accu Reader JA mmm JAN mmm JIP M965 Front Panal figure 3 Rear panel layout USB port RS232C port Centronics printer port DC fan AC power input socket Ventilator NOM wp Power switch Q3 2 3 4 5 OW Dee ws ZG figure 4 Environmental and electrical conditions The instrument has been designed to operate and meet the specifications on previous pages under the electrical and environmental requirements listed below Line voltage 100 240Vac 50 60 Hz Environment conditions Ambient temperature from 15 to 35 relative humidity from 20 to 80 and maintained at 5 Powering up t
3. by UP DOWN ENTER NEXT key For those samples smaller than threshold their labels are reversed DOUBLE LIMIT Two threshold values can be keyed in and high threshold need be greater than low threshold Threshold label If the sample value is greater than above high threshold its label or name can chosen as or by UP DOWN ENTER NEXT key For samples smaller than low threshold their labels are reversed Those values between high and low threshold are labeled CALCULATION User can use simple equations to calculate threshold limit Enter threshold no Enter the number of threshold equations with range from 1 to 4 Enter coefficient There are three coefficients a b and c to be entered for each equation Values are entered by numerical keys and ENTER for confirmation User can move cursor by UP DOWN key After complete keying in three factors press NEXT to next equation The calculated result of threshold equations need be arranged in decreasing sequence i e equation 1 equation 2 equation 3 equation 4 Label of limits The labels between each threshold are displayed These label sequence can be reversed by selecting REVERSE and pressing ENTER Selecting CONFIRM and press ENTER for next step WELL MAPPING Well mapping method In this screen figure 12 below a grid representing 96 well plate is lying on left side User can move around the cursor by UP DOWN LEFT RIGH
4. Point to point Cubic spline Two parameter logit log Four parameter logistic Select one among them by UP DOWN ENTER NEXT Select X axis scale The horizontal axis of fitted curve on printed report can be either linear or log10 scale Select Y axis scale The vertical axis of fitted curve on printed report can be either linear or log10 scale Quantitative standard no Enter the total number of standard to be put in plate for current protocol Quantitative replicate no Enter the number of replicate for each standard Standard auto mapping Select auto manual mapping of standard by YES NO Standard direction If the above answer is YES user can select auto mapping direction for all standards COLUMN allow standard number be arranged in column direction Replicate direction For auto standard mapping the replicate direction of each standard is selected here Note Please refer to figure 11 above for well mapping 23 Select standard type User can select concentration or dilution of standard At this time only concentration is available Enter concentration In this new window all concentrations are displayed here Enter the concentration of each standard with magnitude gt 0 0001 User is to press UP DOWN to scroll the cursor in current and next previous page Press 1 9 and to input concentration value then confirmed by ENTER Press NEXT for following step 2 STORED CURVE Stored
5. Windows windows Update bet you set up how Windows connects lo windows Uodas lor divers Driver Signing Windows Update Profle Hardware profiles provide a wey for to set up and store different configuration Hardware Profiles ar IL Ges Figure A 1 40 uum IDE ATAJATAPL contreters Mea porting descas 1 Communications Fort COMI Communications Pett COM2 Sound video and game controls System devies Universal Seria Bus cantrallers Figure A 2 Figure A 3 41 Figure A 4 42 Appendix B Setting of Hyper Terminal 1 Upon completing appendix procedure move cursor in Windows main screen to Start gt Programs gt Accessories gt Communications gt HyperTerminal figure B 1 2 When the HyperTerminal window opens figure B 2 enter a new name and pick an icon Then click OK 3 Select proper com port number from the Connect using popup menu figure B 3 Then click OK to enter COM properties setup figure B 4 4 Enter 57600 8 None 1 None then press OK 5 In figure B 5 move cursor to Capture Text under Transfer of main bar then select it 6 User is to create a file name for storing transferred data from M965 965 machine as shown in figure B 6 Then press Start to initiate data collection job After the machine has completed measurement move cursor back to Capture Text and select Stop
6. above step is YES Select the reference filter with the same procedure as that of main filter Refer filter 2 This option will come up only at two point mode Select the reference filter with the same procedure as that of main filter Enter time interval This option is for two point mode only Enter the time duration between first measurement and second measurement Need shaker If the plate needs shaking before reading select YES Shake time This option will come up only if the above step is YES Enter proper number and go to next step Shake speed Three speeds are available for shaking The shaking frequency of high speed is 14hz medium speed is 11hz and low speed is 8hz Starting method When plate moving into the machine and before initiating reading it may need stay there for a short period or observe OD change to threshold of designated well then start plate measurement There are three options to select immediate delay time and OD check Delay time If the above option was chosen as delay time user need to key in number here Which well to check If the above option is OD check enter well number here The first letter is entered by subscript under key 1 8 i e AH then press right key to move cursor to next position Enter numeric value by key 0 9 then enter RIGHT LEFT 0 9 key Finally press ENTER to confirm ABS trend Select INCREASE or DECREASE if the abs value of above well is gradually increasing or d
7. are scrolled by pressing UP DOWN and displayed at cursor position Press RIGHT LEFT to move cursor to next previous position Numeric characters are pressed on key 0 9 Press ENTER after entering name or NEXT without correcting name and jump to following step Press ESC to delete current displayed characters Save protocol Previous input options can be saved in ROM under above id and name If no saving is taken current protocol still can initiate the plate reading after leaving editing job Auto save plate data This option will show up only when above protocol has been saved If the data measured by current protocol need be stored in ROM automatically after each test select YES Or the machine will ask user to make choice after each measurement refer to last section for starting test Note data name by auto saving method is defined as Pxx MDYHMS Where xx is protocol id M is month D is day Y is year H is hour M is minute and S is second The time when the data was taken is its name 28 Leave protocol edit Select YES to leave current editing job and following instruction at last section for starting test or NO and return to the beginning of parameter input Note User can press PREV and jump back to last option step during editing process If no modification is required for any option press NEXT and jump to next option step In new protocol creating user can press STOP to quit and return t
8. curve This option is only active when there are standard curve equations being saved in ROM If this option is selected a list of saved curves is displayed for user to select User can press UP DOWN RIGHT to locate the curve then press ENTER to confirm and go to next step The selected equation will be applied to sample concentration calculation Note The curve name of stored equation has following types Cxx LINEAR Cxx_QUADRATIC Cxx_CUBIC Cxx POINT Cxx SPLINE LOGIT LOG Cxx_LOGISTIC Where xx is serial number ranging from 01 to 99 3 STANDARD LINE Standard line If STANARD LINE was selected at quantitative assay user can key in coefficient A amp B of displayed equation All sample concentration calculation of current plate is based on it Use UP DOWN to move cursor and 0 9 amp to enter number Entered number is confirmed by pressing ENTER Press NEXT for net step 14 CONC FACTOR Enter conc factor This option is similar to above step but with only one factor to be keyed in Need cutoff Select YES for sample qualitative analysis or go to step Well 24 mapping method by NO Cutoff methods There are three options here to select by UP DOWN ENTER key SINGLE LIMIT Only one threshold value can be entered by numerical keys and ENTER to confirm Threshold label If the sample value is greater than above threshold value its label or name can chosen as or
9. make sure parameters of machine and printer are properly set up These parameters will be saved in ROM Secondly a generated protocol need be called up and its content displayed on main screen Then prepare a test plate place it on plate carrier and initiate the carrier motion for measurement MODE DATA PROT SETUP MAC DATE TIME FILTER INCUBATOR HEADER figure 7 1 Machine setup For a brand new machine user is to move cursor to MACHINE under menu bar SETUP Then press RIGHT to display sub window figure 7 There are four 12 parameters to be entered in this small window as described bellow Date Time Move cursor up down to this position and press ENTER to switch to a new box User can key in number with 0 9 or delete number with ESC then ENTER to confirm the keyed number Press NEXT or RIGHT to move cursor to next position for further key in Press PREV anytime to return to last main window The updated time will be shown on bottom right corner of screen Filter Move cursor to this position and press ENTER to enter into a new box There are six locations to be entered and they are related to filter wheel by FO F5 For example FO is for marked on wheel surface The default filters are located at FO and F1 Press UP or DOWN to move cursor to the filter number you want then enter wavelength by pressing 0 9 or ESC then confirm it by ENTER The magnitude of wav
10. the main window move cursor to STD CURVE under menu bar DATA and press RIGHT to enter a small window for PRINT and DELETE function Select PRINT and press ENTER to get a window showing all names of saved calibration curves up to maximum 20 sets Please refer to section STANDARD PROTOCOL CREATING for the definition of curve name User can select the desired curve by UP DOWN key or RIGHT key if curves are more than one page Press ENTER to print out the content of this curve for review Press PREV to get back to main screen or repeat printing job Select DELETE and press ENTER for saved curves erasing Make sure not deleting those curves which have already been selected by existed protocols 33 MAINTENANCE Routine care Very little maintenance is required to keep the instrument in good working condition The interior should be kept as dust free as possible and the plate carrier cleaned regularly wipe off spilt chemicals immediately WARNING Do not remove the cover of the instrument CAUTION Do not allow moisture to leak into the instrument The exterior of the instrument can be cleaned periodically as follows Switch off first the instrument and disconnect from the mains supply Usinga lint free cloth dampened with a weak solution of detergent and water wipe the exterior surface of the instrument as necessary Drythe surface with another soft cloth Lamp replacement CAUTION Switch off an
11. ER and the printer will output well mapping of generated protocol not include simple protocol that was latest displayed on screen The printer may output nothing even if machine stored protocols Protocol copy At the main window move cursor down to UTILITIES under menu bar PROT then press RIGHT key to a sub window Select COPY and press ENTER to enter another window Move cursor around in current window by UP DOWN or RIGHT if protocol number is more than 12 If you picked the one to be copied press ENTER Key in id and name for new protocol and new id can not be the same as stored protocol After complete this process the protocol list will come back again User can check whether the protocol just generated is located at last line of screen or 30 not User can make another copy here or press PREV and return to main screen Protocol delete At the main window move cursor down to UTILITIES under menu bar PROT then press RIGHT to a sub window Move to DELETE and press ENTER to enter another window Move cursor around in current window by UP DOWN or RIGHT if protocol number is more than 12 If you picked the one to be deleted press ENTER and that protocol disappeared User can delete another protocol here again or press PREV and return to main screen Hot key At the main window move cursor down to UTILITIES under menu bar PROT then press RIGHT to a sub window Move to HOT KEY and p
12. Excel Please refer to appendix A amp B for further details 4 Data display Move cursor to SHOW DATA under menu bar SETUP then press RIGHT to a sub window The measured original data except for kinetic mode for each test can be displayed on for users to check Move UP DOWN and press ENTER to confirm selection with mark S on right side of YES to enable this feature After running a plate test the reading of column AH of the first row are shown on Icd Users can press UP DOWN key to increase decrease row number and get its 14 column reading If the test has more than one plate reading press RIGHT LEFT to select the plate number wavelength also displayed and its reading After completing data checking press ESC for next process 5 Protocol Data Hot key Before running a plate reading related parameters need be set up under a protocol This protocol can be saved in ROM by standard protocol programming method described in section STANDARD PROTOCOL CREATING or simple protocol programming without saving explained below Only those raw plate data run by standard protocol can be saved in ROM described in section DATA PROCESSING User can also relate hard key 0 9 to stored standard protocols It is easy and convenient to run a routine test by simply pressing assigned hard key described in section STANDARD PROTOCOL CREATING 6 Simple protocol programming In this editing process the BLANK is se
13. K CK OK OK KK KKK KKK OK KK kok 5K 2K OK GE OK 2K OK 5K 2K 2K RE OK OK kokk 3K 2K 5K 2K OK IK 2K 3K 5K 2K KOK OK OK OK OK KK 2K 2K 2K E OK OK 39 Appendix Setting of USB Serial Port If your Windows XP 98SE PC has USB ports only please install the attached USB to RS232 driver on PC Connecting the USB cable between PC and M965 965 machine then power it up Please set up your Windows XP computer as follow Under Systems Tasks of My Computer screen press View system information In System Properties select icon Hardware and press button Device Manager figure 1 In Device Manager double click USB Serial Port figure 2 to enter port setting figure A 3 Please remember the com port number for further use in appendix in 57600 8 None 1 None in those blanks Press button Advanced to enter advanced COM setting figure A 4 USB transfer size is to be selected as max number 4096 Latency timer is to be smallest number 1 Upon finishing above procedure please go to appendix B for further operation System Properties System Restos Updates a Remis Ganera Computer Hadwxe Advancad Device Manager Ue The Davies Manager bets ali lhe hardware davises installed Ed vaut compu Use tra Davics Managal lo change te Of Dence Manager 25 Driver Signing leta vou make sule ha tiated dives are compatible with
14. N Meferfech Provide Accurate Results Operational Manual AccuReader M965 965 Metertech Inc Version 1 12 AA Metertech 63 2 Cheng Gong Road Sec 1 Nangang Taipei Taiwan R O C Tel 886 2 2783 2854 Fax 886 2 2783 1764 www metertech inc com info metertech com tw Metertech Inc provides this publication as is without warranty of any kind either express or implied including but not limited to the implied warranties of merchantability or fitness for a particular purpose All rights are reserved including those to reproduce this publication or parts thereof in any form without permission in writing from Metertech Inc Information in this publication is subject to change without notice and does not represent a commitment on the part of the vender Any errors or omissions which may have occurred in this publication despite the utmost care taken in its production will be corrected as soon as possible but not necessarily immediately upon detection Copyright Metertech Inc 2007 Metertech Inc 63 2 ChengGong Rd Sec 1 NanGang Taipei Taiwan ROC E Mail Info metertech com tw Web Site www metertech inc com Tel 886 2 2783 2854 Fax 886 2 2783 1764 Contents Installation Unpacking Instrument description Instrument front view Instrument internal components Keyboard and display layout Rear panel layout Environmental and electrical conditions Powering
15. T and the coordinate of cursor is shown on upper right corner of screen The upper left corner is position A01 which is the same as that of well plate Keep on pressing key 1 or its subscript A will change well type among Blank Positive Negative Sample and Control The serial no and auto manual mapping of each type are also shown below it If the well is preset as auto mapping item B amp C disappear For manual mapping 25 toggle key 2 or its subscript B allow user to select well arrangement direction column row and toggle key 3 or its script C is to select well replicate direction column row Create mapping To start a well mapping first select the well type by pressing 1 then press 2 and 3 to select direction if the well is manual mapping For manual mapping move cursor to the location where you want to set the first well then press ENTER to confirm that position and the cursor becomes If you need more than one well press RIGHT DOWN to expand cursor zone or after that to reduce zone by pressing LEFT UP If the total well number in the zone is you expect then press ENTER again The cursor zone now is filled with letter Z P N T or C and one cursor Also below the well coordinate an abbreviation well type serial no replicate no for the well being taken by cursor is displayed Z01 1 in the figure below The next serial number to be set for the selected well is also displayed 9 down Man
16. TER or NEXT and jump to next item for input Press ESC to delete current displayed characters Press 13 PREV and return to main window 2 Printer setup Move cursor to PRINTER under menu bar SETUP then press RIGHT to a sub window There are two types of printer can selected EPSON dot matrix and SEIKO thermal printers Move UP DOWN and press ENTER to confirm selection with mark S on right side of that printer Printer can also be set ON or OFF by toggling ENTER So the printer will not print out any data if set OFF even though it is connected to the machine Note Available Epson printer types are LX 300 LQ 300 Available Seiko printer type is DPU 414 If other types of printer were connected the graphic curve may not be printed out properly 3 Data to PC Move cursor to COMPUTER under menu bar SETUP then press RIGHT to a sub window The measured data for each test can be down loaded to pc through RS232 port or USB port Move UP DOWN and press ENTER to confirm selection with mark S on right side of that port Move cursor down again to XXX DATA then repeatedly press ENTER to see display change among END DATA RAW DATA and MIX DATA User can select transferring calculated data only end data or unprocessed data only raw data or combination of end and raw data The data measured is stored at hyper terminal of pc and stored data can also be processed under Microsoft
17. Thirdly either press CLEAR to delete selected area or press ESC to cancel this action After complete mapping job press NEXT or PREV to next or previous step Printing options User can determine printed report content of current protocol after execution here Press UP DOWN to move cursor from current window or move to next page and press ENTER to next window for other selections Once cursor was moved to an option without N this option is Not available at right side press ENTER to select it and a star mark is shown at right side The number of N appeared on window depends on the measurement mode and its related options that user selected for current protocol 27 User can select more than one option at the same time so the printer will also print all them out If current option is not needed press ENTER to remove the star mark After complete selection press NEXT to next step Note Available printing options are protocol summary plate map raw abs matrix blanked result calibration curve residuals of standard concentration concentration matrix kinetic curve kinetic matrix Key in protocol ID Each protocol has its unique protocol id number User can enter new id number from 0 to 99 by pressing 0 9 or just press NEXT to keep original number at protocol modification Key in protocol name Maximum seven alpha numeric characters can be entered for protocol name Those 26 letters
18. d disconnect the instrument from the mains supply and allow the lamp to cool down at least 10 minutes before proceeding WARNING Do not touch inner surface of lamp cup with bare fingers Turn off and unplug the instrument Allow the lamp to cool for at least 10 minutes Loose four knobs on both sides of machine and lift up completely the top cover 4 Open the lamp cover on top of lamp house by loosing two knobs in counterclockwise direction figure 15a 5 Use cross type screw driver to remove two screws down at base of lamp support figure 15b 6 Pull out and up slowly the old lamp and lamp support from black tube 1 in figure 15c and housing Be careful the lamp cable not getting stuck while pulling up 7 Remove old lamp from socket fixed on lamp support as shown in figure 15d 34 figure 15 figure 15b figure 15c figure 15d 35 8 Plug a new lamp 3 securely into the holes 2 of socket Position lamp and lamp support roughly in front of black tube and put two screws back into original holes Do not tighten them yet Check and adjust lamp cable so the cable would not get jammed or twisted 9 Pull lamp support and lamp cup in the horizontal direction so the circular edge of cup fit into black tube completely Fasten those two screws now 10 Put the lamp cover back to top of housing and tighten two knobs again Filters change WARNING The filter holder can be changed while power is on D
19. ecreasing with respect to time OD limit This is the threshold of above abs trend Once greater less than this limit the machine starts to read plate How many samples Enter the maximum number of sample to be put in 20 figure 11d 6 samples row 2 replicates row plate for current protocol 21 How many replicates Enter the number of replicate for each sample Sample auto mapping Select auto manual mapping of samples by YES NO Sample direction If the above answer is YES user can select auto mapping direction for all samples COLUMN allow sample number be arranged in column direction Replicate direction For auto sample mapping the replicate direction of each sample is selected here Note Please refer to figure 11 above for well mapping in different directions There are six samples with 2 replicates each on the microplate Samples are set to be started at well A03 and filled the plate automatically KINETICS Enter measuring no This option is for kinetic mode only User is to enter total sampling number of entire process Enter time interval This option is for kinetic mode only User is to enter time interval between two successive samplings Which well to display data amp curve This function allows real time data and curve to be displayed on screen while executing kinetics so user can make decision from data variation whether to complete this measurement or quit the work right away The disp
20. el 1 Dust cover 1 CD for AccuMate and USB driver Instrument description M965 965 is an eight channel vertical light path photometer with one reference channel designed to perform end point two points and kinetic measurement of microplate under microcomputer control The wavelength 340 900nm is selectable up to maximum six interference filters held in a filter wheel M965 965 possesses following features Graphic dot matrix LCD module suitable for well mapping Incubator designed by a digital temperature controller Easily mounting dismounting mechanism between interference filters and filter wheel 4 Lamp lightened only during measurement to extend its life time Protocol data and fitted curve stored in ROM Quick plate reading in continuous and stepping motion Instrument front view Top cover Keyboard and LCD screen Plate carrier Front door opener UT LG e Es fixing knobs figure 1 Instrument internal components Lamp house Filter wheel Main board with cover Power board Fiber optic tube Incubation chamber SU OY UL At M Ze PIN i figure 2 Keyboard and display layout 1 LEFT ARROW key 2 RIGHT ARROW key 3 UP ARROW key 4 DOWN ARROW key 5 0 9 amp key 6 subscript key 7 OPTION key 8 PREV key 9 NEXT key 10 ENTER key 11 ESC key 12 CLEAR key 13 START key 14 STOP key 4 gt Move the cursor one place to the left
21. elength must be greater than 400 nm for M965 and 340 nm for 965 Then open top cover of machine insert the filter set at the location of wheel hole refer to section MAINTENANCE for filter change Press PREV the machine will start do lamp tuning on each filter which was written in the window box If there is no filter mounted there an error message Filter error will tell which location of wheel has no filter User need either mount it on or delete that wavelength by pressing CLEAR then press PREV again Not until proper correction has been done can it then return to main window Incubator In this new window box select YES by pressing RIGHT or LEFT then press ENTER The cursor location is to enter setting temperature from 15 50C After confirming by ENTER the bottom left corner will display incubator temperature in real time If no heating is necessary select NO and press ENTER to display None and no temperature displaying at the screen corner Press PREV to return main window with current temperature shown at corner if incubator was set up Header In this box user can key in test title and operator name with max 10 alpha numerical characters each Title and name will be shown on heading of printed report Those 26 letters are scrolled by pressing UP DOWN and displayed at cursor position Press RIGHT LEFT to move cursor to next previous position Numeric characters are pressed on key 0 9 Press EN
22. ement time Measurement mode Light source Photodetector Shaking Incubator Plate type Display Keyboard Printer interface Data storage Computer interface Dimensions Weight Power supply Operating conditions M965 400 900nm M965 340 900nm Max 8 selectable filters in a filter wheel 10 2 nm half bandwidth filter center wavelength accuracy 2 0nm 0 000 4 000 Abs 0 001 Abs 0 005 Abs or 2 whichever is greater from 0 000 to 3 000 Abs lt 0 2 CV from 0 000 to 2 000 Abs lt 0 5 CV from 2 000 to 3 000 Abs 1 from 0 000 to 3 000 Abs lt 5 seconds at continuous reading End point two point and kinetic Halogen lamp 12V 20W 9 silicon photodetectors Three user selectable modes 8Hz 11Hz and 14Hz Ambient temp 3 C to 50 C 96 well plate 8x21 character graphic dot matrix LCD module 25 key tactile membranes Parallel Centronics 100 sets of protocol 50 sets of data and 20 sets of standard curve RS232C or USB serial interface W355 x D345 x H174 mm 14 Kg 100 240V 50 60Hz 90W max 15C to 35C RH 90 max Specifications subject to change without notice 11 OPERATION Every time after powering up the machine the cursor will stay at END POINT S of main screen User can use DOWN LEFT and RIGHT keys to move cursor around the main screen MODE DATA PROT SETUP END POINT TWO POINT KINETIC PROTOCOL figure 6 To run a test firstly
23. ge rate of sample reading change is calculated at this mode 7 Start test with simple protocol After leaving simple editing the main screen will display those edited parameters for checking also the plate carrier moves out of machine If there is mistake to be corrected for parameters setting or user want leave this window please press RIGHT or PREV key Place the 96 well plate on plate carrier and make sure the marked edge well A01 of plate is facing front right direction while sliding in figure 8 Press START and the machine will retract the plate carrier for measuring When completing test the machine will move it out again and wait for another test input The printer then start to print data with message Printing and it can be stopped any time by pressing STOP An error message Printer error will show up if printer is not properly set up Press STOP to eliminate this message and proceed to next operation Note During plate measuring process user can press STOP to quit current work and the plate carrier will be moved out right away Toggling OPTION key allow plate carrier to be moved out of into machine only when main screen is displayed When error message please refer to page 38 is displayed press STOP for next operation OOO0000 OCH 0 dre d IOK 3 H 263 KSERKKE SOOKE figure 8 8 Start test with standard protoc
24. he instrument 1 2 Place M965 965 solid and level surface in dry clean environment Leave an open space at least 50mm distance from rear panel and side walls to guarantee air flow around the instrument Connect the female end of power cord into the mains power socket and plug the power cord into a grounded wall outlet with the appropriate voltage Connect the Centronics parallel printer to the instrument at the print port Turn on the power switch waiting 3 seconds for internal power supply active and LCD will be lighted up The machine starts to do initialization figure 5 and finally stay at main screen figure 6 in next section waiting for user input Please follow instructions at next section for proper operation ELISA READER 2 20 Metertech Inc Initializing figure 5 During system initialization several jobs are taken checking external RAM condition on main board checking battery status on main board checking plate carrier motion checking filter wheel rotation tuning lamp voltage and preamp gain for mounted filters Note If a warning message Filter error or Filter empty shows up during lamp tuning the instrument can still arrive at main screen Please get into Machine setup for filter correction explained at next section 10 Specifications Wavelength range Interference filters Measurement range Resolution Accuracy Precision Linearity Measur
25. layed curve can be printed out The first letter is entered by subscript under key 1 8 i e AH then press right key to move cursor to next position Enter numeric value by key 0 9 then enter right left key numeric key Finally press ENTER to confirm Kinetic calculation There are six calculation methods for the obtained kinetic data and can be categorized by rate change max abs and time to max rate abs Press UP DOWN to move cursor from current window or move to next page and press ENTER to next window for other selections Once cursor was moved to the required calculation method press ENTER NEXT to select it and go to next step Note Calculation methods are listed as average rate maximum rate maximum abs total delta abs time to maximum rate 22 time to maximum abs QUANTITATIVE Need quantitative If current test requires quantitative assay select YES go to step Need cutoff by NO Quantitative assay There are four options here to select by UP DOWN ENTER NEXT They are described under titles 1 curve on plate 2 stored curve 3 stanard line and 4 concentration factor 1 CURVE ON PLATE Select data fitting If CURVE ON PLATE was selected at above step standard solution with known concentration need be prepared for current test Concentration and read abs can be fitted by seven different curves i e Linear regression Quadratic polynomial Cubic polynomial
26. n one page then press ENTER The machine will start process it by a stored protocol with the same id as data file has After process is completed the printer will output the result if it was set ON If this protocol was deleted the machine stop working and screen will display No Proto User may modify this protocol maintain the same id to get different processed result from the same raw data Data copy At the main window move cursor to COPY under menu bar DATA and press ENTER to enter another window with data files listed Select the required raw data and press ENTER and enter new window for entering new data name After key in alpha numeric letters press ENTER and get back to data table The new generated data file is at last line of listed table Press PREV to get back to main screen or repeat copying job 32 Data delete At the main window move cursor to DELETE under menu bar DATA and press ENTER to enter another window with data files listed Select the required data file and press ENTER to delete it Press PREV to get back to main screen or repeat deleting job Data print At the main window move cursor to PRINT under menu bar DATA and press ENTER to enter another window with data files listed Select the required data file and press ENTER to print it out make sure printer was set up correctly Press PREV to get back to main screen or repeat printing job Standard curve At
27. not connected to machine or not powered up No proto The appointed protocol for data to be processed did not exist Proto memory full Total number of protocol in ROM exceeds 100 sets Delete old or useless ones before further protocol creation Curve not existed The standard curve selected by current protocol was deleted Edit this protocol again with another stored curve Threshold sequence error The calculated results of threshold equations are not arranged in decreasing order Correct their coefficients again with proper magnitude in cutoff setting Well not mapped None of sample T and standard C is placed on the microplate during well mapping process User must fill in either one for proceeding to next step Mathematic error While executing quantitative function keyed in standard concentration of designated well is not related to the absorbance of that well ex the standard OD is lt 0 005 or lower asymptote Press STOP to eliminate this message and correct the standard mapping in protocol setting Filter wheel error The wheel motor or photo interrupter or motor driver may be malfunctioned Plate motion error The stage motor or photo interrupters or motor driver may be malfunctioned DKK KK OK KK 5K 5K 5K OK 5K 3K OK 3K OK OK OK 5K OK KK 3K KK K KKK K KKK KKK KKK KKK OK KKK KKK 2K OK OK OK 5K OK OK OK OK OK OK OK KOK Note When error messages are displayed please press STOP for next operation DKK KK A
28. o main screen at any option selection In editing an existed protocol press STOP during option selection will jump to option Save protocol and continue remaining work 29 PROTOCOL MANAGEMENT Standard protocols generated by last section are saved in ROM with maximum 100 sets These protocols can be modified deleted copied to another name and printed out Protocol edit At the main window move cursor to EDIT under menu bar PROT refer to figure 10 in last section Then press ENTER to get into another window All generated protocols are listed here Each protocol is represented by XX YY where XX is id number and YY is protocol name Move cursor around in current window by pressing UP DOWN or RIGHT if protocol number is more than 12 If you have picked the one to be modified press ENTER In the next window select YES to confirm it or NO and return to main window The following procedure is the same as creating protocol described in last section However all default values of related parameters are replaced by current protocol Protocol print At the main window move cursor to PRINT PROT under menu bar PROT Then press ENTER and the printer will output created protocol parameters not include simple protocol that was latest displayed on screen The printer may output nothing even if machine stored protocols Map print At the main window move cursor to PRINT MAP under menu bar PROT Then press ENT
29. o not touch any other electronic components to reduce risk of shock 1 Loose four knobs on both sides of machine and lift up completely the top cover 2 Rotate the filter wheel by hand until the wheel hole number to be used is shown right up on left side of wheel hole 2 in figure 16 3 Ifthere is filter holder at this hole remove it by pulling straight up Then insert a new filter holder with its wavelength characters 1 oriented in the direction as shown in this picture The filter hole won t accept filter holder if it is reversely inserted 4 For a successful insertion the filter holder is not easy to be removed and won t drop or loose during wheel rotation figure 16 36 figure 17 37 Optical mechanical parts cleaning WARNING The machine need be periodically cleaned on its optical parts The contaminant dropped from test samples and sticking on optical mechanical parts may reduce reading accuracy and affect plate motion smoothness 1 Turn off and unplug the instrument 2 Open up the top cover of machine as mentioned above 3 Loose four screws 1 in figure 17 above on both sides of heater cover 3 Then remove the heater cover carefully from the machine 4 Loose and remove three screws 2 on top of top heating plate 4 Move plate up slowly and turn it over to left side of machine Then lay this plate down on top of heat sinks 5 There are wires connected between heating plate and machine Plea
30. ol If user want to run protocol stored in ROM move cursor down to PROTOCOL under menu bar MODE Press ENTER and select required protocol in listed table Press UP DOWN to move cursor at current screen or press RIGHT to next screen if more than 12 sets of protocol are saved Press ENTER to confirm selection and parameters of this protocol are displayed under menu bar figure 9 The plate carrier is also moved out of machine Press UP DOWN to check more parameters covered by cursor The following procedure for running a test by selected protocol is the same as that in simple protocol test above At the end of measurement test the screen shows Save current data if the protocol was preset as manual saving of data User can make decision by checking the printed data Maximum seven alpha numeric characters of data name can be keyed in if YES was selected MODE DATA PROT SETUP Protocol P25 AABBOO1 Mode Kinetic Motion Continuous Filter M1_405 11 10 24 figure 9 If a standard curve was fitted in the test the screen also shows Save standard curve By checking the printed curve and equation user can let the machine save this curve by a given name with selectable ID 01 99 or just leave there without saving refer to page 24 for curve name definition The saved name will be displayed on screen for user to remember After that press STOP and return to main screen Note Totally 20 se
31. ontinuous or stepping method Available keys are the same as above step Note Continuous motion has quicker reading however stepping motion get better reading for flat bottom well with high OD samples For U bottom or round bottom well continuous motion get better reading than stepping motion for all measurable OD range Enter stepping delay if stepping motion is selected user can key in delay time between each row reading of plate Press 0 9 and ESC to enter proper number within the range hinted at the bottom of LCD screen Press ENTER or NEXT and confirm displayed number The default delay time is 200 msec for liquid sample surface stabilization Need incubation If the plate need be heated during measurement select YES 19 Incubation temp This option will come up only if the above step is YES Press 0 9 and ESC to enter proper number within the range hinted at the bottom of LCD screen Press ENTER or NEXT and confirm displayed number Main filter 1 Press ENTER and enter a new window box Press UP DOWN to move cursor among displayed filters Press ENTER to select it and return to previous window Press NEXT to next step Main filter 2 This option will show up only at two point mode Select the filter with the same procedure as above step Need refer filter If there need be a reference filter during measurement select YES Refer filter 1 This option will come up only if the
32. ress ENTER to enter another window with hot key table Select required number 0 9 by UP DOWN then press ENTER and enter protocol table Select the protocol then press ENTER and get back to hot key table The selected protocol id is showing after picked number Press PREV to return to main screen or repeat current work User can delete any protocol id existed in hot key table by press CLEAR Note If user deletes the selected protocol by the method explained above and get back to this hot key table He will find out the protocol id after assigned hot key number is replaced by N A message It means this hot key number is no longer available for the deleted id User can press CLEAR to erase this message and select other protocol id 31 DATA MANAGEMENT The raw data obtained by standard protocol execution are saved in ROM with maximum 50 sets These data can be processed deleted copied to another name and printed out MODE DATA PROT SETUP PROGESS PRINT COPY DELETE STD GURVE 11 50 04 figure 14 Two kinds of data name may be used for saving One 15 named by auto saving method refer to page 29 for definition and the other by manual saving refer to page 17 Data post process At the main window move cursor to PROCESS under menu bar DATA and press ENTER to enter another window with data files listed User can select the desired curve by UP DOWN key or RIGHT key if curves are more tha
33. se be cautious not to break or tear off these wires from the plate 6 Pull plate carrier 8 far out of machine slowly so users can easily reach the optical glass 6 7 Use a soft clean cloth or cotton rod dipped with alcohol to clean the surface of glass 8 Then also clean top surface of plate carrier surface of top and bottom heating plates 5 as well as rails 7 on both sides of carrier 9 Put top heating plate back to original position and tighten it by three screws 10 Mount the heater cover above top heater plate Then push this cover inward at horizontal direction and make sure no air gap between walls 11 Tighten four screws then pull front door opener out and pull down top cover of machine finally fix four knobs on both sides Messages This paragraph lists messages for errors or abnormal conditions or warning during machine operation RAM error The external ram is not operating properly call for service Filter empty There is no filter existed in filter wheel Insert required filter FilterX error There is no filter at the X position of filter wheel or the lamp has malfunction Insert new one at that position or clear filter setting at machine setup or change a good condition lamp Battery error The battery on the main board is out of condition or removed 38 Turn off machine and insert proper one Filter not setupFilter setting has not been executed at machine setup Do it Printer error Printer is
34. t as air and all 96 wells on plate are SAMPLE no matter what kind of solution will be there The entire plate will be read regardless actual number of well being taken END POINT S Move cursor back to END POINT S under menu bar MODE Press ENTER to start parameter setting of end point mode In this environment user can select motion method main and reference filter type shake speed and duration Please follow instructions in next section for proper entering selecting above parameters When completing input Exit simple edit will be displayed on screen Select YES to leave current editing work or NO to return to the beginning of parameter input Note User can press PREV and jump back to last option during editing process User can press STOP during option selection to quit current protocol editing and return to main screen TWO POINT S Move cursor to TWO POINT S under menu bar MODE Press ENTER to 15 select motion method main and reference filter type delta time between two measurements shake speed and duration The method for entering or selecting parameter is the same as above approach KINETIC S Move cursor down to KINETIC S under menu bar MODE Press ENTER to select motion method main filter type shake speed and duration sampling number and sampling interval as well as well for curve display The method to enter or select parameter is the same as above approach Note The avera
35. ts of standard curves can be saved in ROM The first saved one will be deleted if curve ROM is full User may find these curves at quantitative editing of protocol or from STD CURVE under menu bar DATA in section DATA MANAGEMENT If users just created modified a protocol following instructions in next section they can start to run a test directly 9 Start test with hot key When the display is showing main screen user can press key 0 9 to initiate a test if the pressed key was related to stored protocol described in section PROTOCOL MANAGEMENT The content of protocol will be displayed under menu bar The rest of following test procedure is the same as above 18 STANDARD PROTOCOL CREATING At the main window move cursor to NEW under menu bar PROT Then press ENTER to start a new protocol creating procedure figure 10 The details are explained step by step below After finishing programming job user can start measurement as described in last section Note For the beginner to operate this machine it s suggested that he start from simple protocol programming as described at last section MODE DATA PROT SETUP NEW EDIT PRINT PROT PRINT MAP UTILITIES gt figure 10 Measure mode user can select one among end point two point and kinetic modes for current protocol Press UP DOWN to move cursor then press ENTER or NEXT to next step Plate motion Select plate carrier motion either by c
36. u Figure 12 shows an example by manual mapping of well The printer output figure 13 of this mapping is rotated 90 degrees clockwise with respect to LCD screen setting Z N E E E E IW E C 20 figure 12 8 blanks column 4 standards column with 2 replicates row and 32 samples column 26 H G F E D C B A BLKO8 BLKO7 BLKO6 BLKO5 BLKO4 BLKO3 BLKO2 BLKO1 01 SAM04 SAMO3 SAMO2 SAMOi STD04 STDO3 STDO2 STDO1 02 SAMOS 5 07 SAM06 SAMOS STD04 STD03 STDO2 57001 03 SAM16 15 SAM14 SAM13 5 12 SAM11 SAM10 5 09 04 SAM24 SAM23 SAM22 SAM21 SAM20 SAM19 SAM18 5 17 05 SAM32 SAM31 SAM30 SAM29 5 28 SAM27 5 26 5 25 06 07 08 09 10 11 12 figure 13 printed mapping of above LCD screen setting For auto mapping move cursor to the location where you want to set the first well then press ENTER The well type only sample and standard are allowed in auto mapping user just picked will fill in the grid with preset number The serial number of these wells is arranged according to predefined direction Note Letters laid on grid are Z for blank P for positive control N for negative control T for sample and C for standard calibrator Delete mapping If the current mapping need be changed or deleted firstly move cursor to any well position with well type which need be deleted Then press CLEAR to show all wells with same type in reverse image
37. up instrument Specification Operation 1 Machine setup 2 Printer setup 3 Data to PC 4 Data display 5 Protocol Data Hot key 6 Simple protocol programming 7 Start test with simple protocol 8 Start test with standard protocol 9 Start test with hot key Standard Protocol Creating Measure mode Kinetics Quantitative assay Cutoff Well mapping method Printing options Protocol saving Leave protocol edit Protocol Management Protocol edit Protocol print Map print Protocol copy Protocol delete Hot key ON KR HR A CJ U U U U N NY NJ MN N N N eae k e k be k PP e e k e O O O O N UA U N O N O Ui U KR HBR HRN e Data Management Data post process Data copy Data delete Data print Standard curve Maintenance Routine care Lamp replacement Filters change Optical mechanical parts cleaning Messages Appendix Setting of USB serial port Appendix B Setting of hyper terminal 32 32 32 33 33 33 34 34 34 36 38 38 40 43 INSTALLATION Unpacking The package contains items listed below Unpack the carton and inspect carefully If any part is damaged or missing contact your dealer immediately Qty Description 1 M965 965 Microplate Reader 1 Power cord 1 Operational manual 2 M965 965 4 interference filters 405 450 490 and 600nm inside the filter wheel M965 5 interference filters 340 405 450 490 600nm inside the filter whe
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