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Electrophysiology & Cell Biology Research

1. Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 283 Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection Harvard Neuro Phore BH 2 System In the past few decades the application of drugs and other dissolved agents from multibarrel electrodes pipettes has evolved into a practical method of testing their effects on cells or cellular systems The versatile Neuro Phore BH 2 System is designed to facilitate controlled ejection of fluids from multibarrel micropipettes Extracellular ejections of minute volumes can be delivered using up to five pumps in serial or parallel The ejection schedule for each pump can be independently programmed for sequential or simultaneous output Ejection cycles can be internally timed triggered manually or synchronized to external events This flexible system allows the use of iontophoretic pump modules pneumatic pump modules or a combination of both By interchanging the IP 2 lontophoresis Pump Module with a PPM 2 pneumatic pressure pump the overall system capability can be expanded for simultaneous pressure and iontophoretic injection of drugs from a multibarrel pipette The Neuro Phore BH 2 System was developed under the guidance of active researchers with extensive experience in iontophoresis techniques These researchers needed a system to provide precise stimulation and quantitative control for ejection of drugs in
2. Chamber Section 5 Overview of Imaging Systems sees 6 7 Chamber Cross Reference Chart rsvsrnvrnrnrnvnenonvnnrnvnrnenenerernrnrnvnenennsesnrnrnens 8 9 Warner Heating Overview s rssvsvsvsvsvsvrvrvnvrnnvrvrvsvrnvvrnsvsvrnvvensrsnensvrnsvsnrnsennnn 10 11 Qocyte NET 12 13 RC Series Chambers Legacy Design nenrnvnrnonenenonvnonenenvnvnenenenvnvnenenenene 14 15 Series 20 Chambers sassinicssinssrinincnsscsvatsvrascasvevssssvavasacvnsvnsuevavaseusteusevenestantaunensne 16 40 Series 30 WE TC 41 44 CV 30 CytoViva Environmental Chamber sosornrnrnrnenrnenenrnenrnenenvnvnrnenvnvnvnensnr 45 Culture Dish ITT CN 46 49 Series 40 Chambers Quick Change Coverslip Bottom Imaging Chambers sorrvrvrvrvrrrvrrrrvrrnrvrrverererererenene 50 Low Profile Chambers sere 51 High Profile and Closed Bath Chambers u c cccccecccscssessesescstecscesececetessseseessseseeeaeaes 52 Slotted Bath Low Profile Chambers eosoerorvrvrrrrrvererererrrrrrsrerernravereverenererersrrrrsrnvenenn 53 Perfusion Chamber with Field Stimulation 0010ssissssiesissiesersirsirsiesrsireierienrenennn 54 Series 50 Chambers for Transepithelial Studies seen 55 56 PFC 1 ProFlow Shear Flow Chamber rnvsvnnnnnrnvnenonvnnrnvnvnenenvrvnnrnrnvnenennsnrnrnrn 57 YC 1 Flow Chamber for Yeast Cells rnrnonnrnrnrnrnenvnvrnrnrnvnenvrnrnrnenenvrenvvnrnrnenenenn 58 Culture Dish Platforms Classic Series 20 Hlatiomms 59 PM Series Heat
3. and Clear functions are not found on other microinjection units Balance In addition to the ejection pressure the Pico Injector offers a secondary balance pressure This secondary balance maintains a positive pressure on the injection pipette before and after injections This eliminates dilution caused by capillary action and aids in the prevention of clogging Wasteful continuous injection which often occurs when no separate balance pressure is offered by an injection device is avoided Fill Hold There are two built in vacuum generators to fill a micropipette from the tip and to hold suspended cells Filling the pipette from the tip is easier than back filling Suspended cells can be held with a second holding pipette The holding vacuums range accommodates most cell types Clear A high pressure pulse can be used for clearing a pipette should it happen to clog This is particularly useful when working with pipettes sized for smaller volumes Electrical Connectors BNC type connectors are available at the front panel to ease integrating the Pico Injector with other equipment Synchronization of injections to other stimulations or recordings is therefore possible Selection Guidelines The PLI 100 is available in three packages Plus Basic and Deluxe The basic unit is supplied with an input hose output hose holding hose power cord and instruction manual The plus unit also includes a foot switch pipette hold
4. 296 296 296 295 295 295 295 300 300 301 micromanipulators gt D E ee T 293 s10 ejndiuewoJoiw MOIEN 294 Micromanipulator Selection Guide Manipulator Selection Guide Chart continued Coarse Ratio Movement Adjustment Travel Motorized Manipulators X 37 mm X Y Z max Y Z 100 um 20 mm max In conjunction with MCL Controller 0 01 um Resolution Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Fine Movement Resolution Travel 10 mm max 25 mm max X Y Z 100 um X Y 25 mm max X Y Z 25 mm max X Y Z 25 mm max Resolution Mounting 0 5 um 1 um NN 0 5 um X Y 1 um X Y Z 1 um 25 nm 7 mm Clamping Bolt Parallel to Working Axis 12 mm Clamping Fitting 10 mm Clamping Width 10 12 mm 0 5 in Rod Clamp Tilting Base Integrated 25 Tilting Base Integrated 25 Tilting Base Features Options DC Motor Controlled DC Motor Controlled Can use either Joystick or Push Button Controller Velocity of lt 36 mm sec 40 000 steps um revolution Velocity of lt 36 mm sec 40 000 steps um revolution Designed for Electrophysiology Applications Order W4 73 0003 W4 73 0011 W4 60 0571 W4 60 0572 W4 60 0574 W4 60 0600 W4 60 0601 W4 60 0586 W4 60 0588 W4 60 0590 W4 60 0592 W4 64 1731 W4
5. A clear button resets the position to zero Movement may also be made by joystick These joystick movements are also monitored and may be both displayed on the controller and read via the RS 232 interface The Controller may be used either alone or computer driven via the RS 232 interface Power 115 230 VAC 50 60 Hz Ultraprecise Manual Control Micromanipulator The base of the manual micromanipulator is in keeping with the mass and stability of the entire instrument It is 14 x 14 cm 5 1 2 x 5 1 2 in has a three point rest and is bored for four M6 bolts The large diameter micrometer spindles on each axis have fine adjustment only with 25 mm 1 in of travel that can be read direct to 5 um and interpolated to a 1 um positioning resolution The entire micromanipulator can be tilted forward up to 25 so that cells can be penetrated along the axis of one of the drives The same locking screw that controls the tilt also provides coarse height adjustment The probe holder has multiple locking swivels allowing it to be positioned at any angle relative to the X Y or Z axes ultraprecise micromanipulator is available with motorized control on either two or three axes for essentially vibration free steps as small as 0 01 mm e Available with manual control or motorized control W4 60 0594 Ultraprecise Manual Control Micromanipulator Micropipette Not Included Ultraprecise Motorized Control Micromanipulator With Stepper Motors Contro
6. India 400 088 phone 91 22 2551 6908 fax 91 22 2556 3356 e mail info marsap com ISRAEL New Biotechnology Ltd PO Box 8662 Jerusalem 91086 Israel phone 972 2 6732001 fax 972 2 6731611 e mail nbtsales nbtltd com website www nbtltd com ITALY Crisel Instruments Srl Crisel Instruments Srl Via Mattia Battistini 177 00167 Roma Italy phone 39 06 35402933 fax 39 06 35402879 e mail becattini crisel instruments it website www crisel instruments it o JAPAN Take In Incorporated Kyosu Building 3 31 11 Amanuma Suginami Tokyo 167 0032 Japan phone 81 3 3597 1911 fax 81 3 5833 5596 e mail take in labtak com website www ha j com H MEXICO Intecs Instrumentaci n S A de C V Prolongacion de la 15 Poniente 3123 Colonia La Paz Puebla Puebla 72160 Mexico phone 52 222 756 2900 fax 52 222 231 5166 e mail ventas intecssa com mx website marcop intecssa com mx NEW ZEALAND Alphatech Systems Limited P 0 Box 62613 Kalmia St Auckland 1544 New Zealand phone 64 9 580 1959 fax 64 9 580 2044 e mail sales alphatech co nz website www alphatech co nz PAKISTAN Bc Meditech Private Limited Meditech House 114 G 1 M A Johar Town Lahore Pakistan phone 92 42 5302643 thru 46 4 lines fax 92 42 5302640 amp 41 2 lines e mail info meditech com pk SPAN Izasa S A C Aragon 90 0815 Barcelona Spain phone 34 902 20 30 80 fax 34 902 20 30 81 e mail dac2 izasa es website Www izasa e
7. Injections and micro aspirations Its compact dimensions enable it to be ergonomically positioned next to the control lever of a micromanipulator to facilitate single handed operation of both instruments Suction injection is achieved by using the rotary actuator with a fine micrometer thread which has a 15 mm movement range A 1 ml gastight Hamilton glass syringe with a Luer taper is incorporated A Luer taper hypodermic needle is mounted on the taper of the Hamilton syringe The syringe is connected by hard polyethylene tubing to a micropipette which in turn may be fitted to a micromanipulator or similar instrument The 1 ml Hamilton syringe can be easily exchanged for any other Hamilton 1700 syringe series including the 1000 500 200 100 and 50 ul sizes visit www harvardapparatus com for complete details Order Product W4 69 0107 Screw Actuated Micrometer Driven Hamilton Syringe Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com PM 8 and PM 4 8 and 4 Channel Pressure Injection Systems W4 69 0153 8 Channel Pressure Injection System e Multiple function system for micro injection and perfusion e Up to 8 injection perfusion outputs one hold cell output and one synchronized drain out channel e Programmable timers counters and step sequences which can be stored for reuse e Manual control or automatic sequential step cycle operation e Front pan
8. MEP F10FL MEP F12FL MEP F15FL MEP F20FL MEP F10T MEP F12T MEP F15T MEP F20T ES port B port FL microelectrode holders gt D E ee T 4 317 SJAp OY 3P01 23J30121W o se z e 318 PE Series PE Series Electrode Holders Microelectrode holders for every application and manufacturer Specifications Body Material PESW BxxP PESW TxxP e Microperfusion perfusion at the pipette tip For microperfusion inside the pipette Perfusion at the electrode tip is possible with the PE Series holders They include an additional port at 30 to the glass bore allowing for the insertion of micro bore tubing into the pipette This port includes a threaded cap and seal for up to 1 mm diameter tubing See references below for information on the microperfusion technique PE SERIES Holders Connector 1mm Pin Order W4 64 1144 W4 64 1145 W4 64 1146 W4 64 1371 W4 64 1147 W4 64 1148 W4 64 1149 W4 64 1150 W4 64 1369 W4 64 1151 W4 64 1160 W4 64 1161 W4 64 1162 W4 64 1373 W4 64 1163 Port Yes Wire Pellet Wire Body Style Straight BNC 1mm pin Threaded Collar Body Style Connectors Coupling Port Perfusion Port Standard Glass Sizes 9 5 mm compatible with MHH 38 Polycarbonate Straight 1mm Pin 1 mm Pin Threaded and BNC Ag Wire 2 mm diameter Polycarbonate 30 port accepts tubing up to 1 mm diameter 1 0 1 2
9. System Ussing Diffusion Stimulators Microscopy Stereo Zoom Microscopes Microscope Cameras Cold Light Sources LED and Halogen Fiber Optic Ring Lights and Light Guides Laser Systems Coverslips Electroporation BTX Electroporation Electrofusion Transfection Transformation Solutions Pulse Generators for In Vivo Electroporation Systems High Throughput Electroporation Monitoring Systems and Specialty Electrodes Ur 9 os ans ae Ee d TS Temperature Electro physiology Electroporation Control Technical Support Ordering Payment Delivery Terms and Conditions Technical Support amp Domestic Orders Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 USA phone 800 599 4203 toll free 203 776 0664 fax 203 776 1278 e mail support warneronline com website Www warneronline com Domestic Orders Harvard Apparatus 84 October Hill Road Holliston MA 01746 1388 USA phone 800 272 2775 toll free 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com website www harvardapparatus com See inside back cover for distributors outside of the United States Payment Options Purchase Order Terms are net 30 for customers with pre approved credit Credit Card Visa MasterCard American Express and Discover are accepted Prepayment To prepay send check or money order with your purchase order Call Harvard Apparatus customer service in advance
10. W4 64 0811 TG200 4 2 0 14 0 2 100 capillary glass gt D ee T Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 325 Patch Clamp Glass Custom 8520 glass has been shown to be equal in noise performance to the discontinued Corning 7552 Premium Corning 7056 Alkali Barium Choosing patch clamp glass is generally based on noise Borosilicate Patch Glass performance and the ability to form and maintain a seal No single type of glass works best in all applications and some trial and error is usually required to find the type yielding optimum results in your experiment Patch glass is manufactured without an inner filament This glass is offered in both the standard and premium models The premium glass has fire polished ends and is cleaned with deionized water before being packed in dust free containers Now Available in Premium Line Corning 7056 glass is now offered in place of the 7052 glass last melted in 1992 and no longer available The 7056 formula has also been Custom 8520 Patch Glass discontinued by Corning last melt in 1995 but stocks of this material are still available The properties of the 7056 glass are similar to the 7052 as is its performance in patch experiments Specifications Composition 68 SG 18 B 0 9 K 0 3 Al 0 1 Li 0 1 Na O Softening Temperature 718 C Now Available in Premium Line 2 The custom patch
11. W4 64 0823 1 5 OSW A15P W4 64 0978 17 QSW A17P W4 64 0824 2 0 QSW A20P 3 W4 64 0841 459 No 1 0 Q45W A10N D W4 64 0842 1 2 Q45W A12N o W4 64 0843 1 5 Q45W A15N S W4 64 1098 17 Q45W A17N a W4 64 0844 2 0 Q45W A20N W464 1055 Yes 1 0 Q45W A10P i z W4 64 1056 1 2 Q45W A12P W4 64 1057 1 5 Q45W A15P o W4 64 0968 17 Q45W A17P W4 64 1058 2 0 Q45W A20P S W4 64 0861 90 No 1 0 QRW A10N W4 64 0862 1 2 ORW A12N Ci r W4 64 0863 1 5 ORW A15N q W4 64 1103 1 7 QRW A17N W4 64 0864 2 0 QRW A20N W4 64 1075 Yes 1 0 QRW A10P W4 64 1076 12 ORW A12P HME W4 64 1077 1 5 QRW A15P W4 64 0973 17 ORW A17P a por W4 64 1078 2 0 QRW A20P W4 64 1351 Hybrid Straight No 1 5 QSH A15N W4 64 1352 1 7 QSH A17N W4 64 1353 2 0 QSH A20N W4 64 1354 Yes 1 5 QSH A15P n N Aa port W4 64 1355 17 QSH A17P GT WA 64 1356 2 0 OSH A20P HP 205 W4 64 1288 with Teflon Sleeve 312 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Q Series Q Series Holders with 1 mm Pin Threaded Collar Compatible with Axon Patch Clamps and Microelectrode Amps post 2 1 95 NOTE Holders without ports are not suitable for patch clamp recording Q SERIES Holders with 1 mm Pin Threaded Collar Glass Order Wire Pellet Connector Port OD mm Model W4 64 1121 Wire Straight No 1 0 OSW T10N W4 64 1122 1 2 OSW T12N W4 64 1123 L OSW T15N W4 64 0749 1 7 OSW T17N W4 64 1124 2 0 QOSW T20N W4 64 0837 Yes 1 0 OSW T
12. website www delcarpio cl CHINA BEIJING DL Instruments Inc A59 2 126 West 4th Ring Middle Road Beijing phone 010 6818 9642 fax 010 6822 2702 e mail azou dongeonline com website www dongleonline com www dongleonline com cn CHINA GUANGZHOU Bioprobes Ltd Guangzhou office Room 116 JinXia Bldg No 15 Shi You Xin Er Heng Road Wu Yang Xin Cheng Guangzhou China phone 20 87357072 20 87357737 fax 20 87357072 e mail info bioprobeschina com website www bioprobeschina com DL Instruments Inc Guangzhou office 4 Siyou New Road Great Wall Building Rm 612 Guangshou phone 020 8765 6735 020 8765 6736 fax 020 8765 6737 e mail azou dongeonline com website www dongleonline com www dongleonline com cn CHINA WUHAN DL Instruments Inc 288 Zhenxing Road Room 301 1 302 Wuhan phone 027 8356 9708 fax 027 8356 9700 e mail azou dongeonline com website www dongleonline com www dongleonline com cn Scandidact Biogimaterie Oldenvej 45 3490 Kvistgaard DK 3490 Denmark phone 45 49 13 93 33 fax 45 49 13 83 85 email admin scandidact dk DENMARK HONG KONG Bioprobes Ltd Hong Kong office 14 F Highgrade Building 117 Chatham Road South Tsimshatsui Kowloon Hong Kong phone 852 2723 9888 fax 852 2724 2633 e mail info bioprobes imsbiz com hk website www bioprobeshk com INDIA Marsap Services Pvt Ltd 29 31 Ujagar Ind Estate WIP Marg Rd Deonar Mumbai
13. 1 5 1 7 and 2 0 mm Patch Clamp Compatibility Connector 1mm Pin BNC 1 mm Pin with threaded collar References For Use With Warner Patch Clamp Models PC 501 amp PC 505 Axon Patch Clamps prior to 2 1 95 List Heka and Dagan Patch Clamps Axon Patch Clamps and Microelectrode Amps prior to 2 1 95 1 Nehr E and Eckert R Fast patch pipette internal perfusion with minimum solution flow Grinnel A D Armstrong D and Jackson M B Calcium and lon Channel Modulation Plenum Press N Y 2 Cull Candy S G Miledi R and Parker l Single glutamate activated channels recorded from locust muscle fibers with perfused patch clamp electrodes J Physiology 32 195 210 3 Pusch M and Nehr E 1987 Kinetics of loading small cells with various compounds by use of patch pipettes Pflugers Archives Spring Meeting of the Physiol Ges 4 Tang J M Wang J and Eisenberg R S 1992 Perfusing patch pipettes Methods in Enzymol 207 176 181 Glass OD mm 1 0 1 2 1 5 1 7 2 0 1 0 1 2 L 1 7 2 0 1 0 1 2 1 5 1 7 2 0 Model PESW A10P PESW A12P PESW A15P PESW A17P PESW A20P PESW B10P PESW B12P PESW B15P PESW B17P PESW B20P PESW T10P PESW T12P PESW T15P PESW T17P PESW T20P port port Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com NEW
14. 500 e High quality borosilicate glass e Available in two diameters Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 329 sse b Mejpideo O SE lt 330 Pipette Sterilization and Micropipette The Pipette storage container is both convenient and easy to use Pipette Sterilization and Storage Container e Ideal for dry heat sterilization of pipettes e Pyrex type glass covers make contents easily visible e Holds 21 pipettes e Available for 1 0 1 2 1 5 and 2 0 mm diameter pipettes This container is a convenient and easy to use pipette storage container It is ideal for both storage and dry heat sterilization of micropipettes In most application pipettes must be free from contamination at all times By storing completed pipettes and partially made pipettes between operations the cleanliness of the micropipettes can be assured Because the covers are made of Pyrex glass the complete container with pipettes in situ can be dry heat sterilized ensuring contamination free pipettes The base accepts 21 pipettes Select from containers holding either 1 0 1 2 1 5 or 2 0 mm OD pipettes Order Product W4 69 0114 Pipette Sterilization and Storage Container for 1 0 mm Pipettes W4 69 0316 Pipette Sterilization and Storage Container for 1 2 mm Pipettes W4 69 0317 Pipette Sterilization and Storage Container for 1 5 mm Pipettes W4 69 03
15. 64 1732 W4 64 1733 Page 303 303 298 298 298 298 298 301 301 301 297 297 297 Standard Manual Control Classic design at a reasonable price Micromanipulators are recognized as classic pieces of research equipment How they work is precision engineering How they feel is art These micromanipulators have set the standard worldwide for decades They are the most popular and widely used Selecting the correct micromanipulator for the application is important If you are buying one instrument for general laboratory use then select the Ultraprecise Micromanipulator see page 301 W4 60 0570 Left Handed Standard Manual Control Micromanipulator with 1 Tool Electrode Holder Micropipettes not included Standard Manual Control Micromanipulator with 2 Tool Electrode Holders Micropipette not included e 20 mm of travel in lateral Y axis and vertical Z axis graduated in 0 1 mm increments e 37 mm of travel in probe direction X axis graduated in 0 1 mm increments plus 10 mm of travel with fine positioning in 0 01 mm increments e All three control knobs located in a single plane for one handed operation and close positioning of several instruments side by side e For use with microscope magnifications up to 250x These micromanipulators have three stacked stainless steel roller bearing raceways providing movement in the X Y and Z axes Rack and pinion drives minimize backlash and provide fast
16. Clamps Amplifiers srvrvrenrnvvnvnvvevrvnenvnvenvnvvrvrenevvenenenverenenennenenns 199 204 Amplifiers Intracellular Electrometer oo ccccccccccsssescsececececsesescsesesesesesecececsesesesesetenenees 205 207 Differential Amplifiers oo ccc cece cccseseseeseseseecececsesescsesesesesscecseecseseseseeecesees 208 210 Low Pass ULT 211 212 Ussing Diffusion Ussing Systems Introduction tere deel ees 213 215 U9500 amp U2520 Ussing Chambers eovrvrnrvrvrrrvrrrerevererererrrerrrerrrrrrrrererereerennr 216 218 Self Contained Ussing Chambers U9926 SUD 219 220 Navicyte Diffusion and Ussing Systems sosrsrvrvevrvrverererrnrvrrrrrrrnrrrnrererernnennr 221 227 EasyMount Vertical Diffusion Ussing Chamber Systems oenrrvrrrvrrrvrvrvesn 228 229 Epithelial Voltage Clamps EC 800 EC 800LV amp EC825A uo 230 231 Multi Channel Epithelial Voltage Clamps VM 232 233 Single Channel Epithelial Voltage Clamp VLC D00 cece ceteteteteeeeeees 234 Input Manifolds and Dummy Membranes DM MC6 EP MC6 DM660 amp DM 235 Computer Controlled Multi Clamp w Software oorrrrrrrnrrvrvvvvverevererererererrvrnnnnnn 236 Data Acquisition amp Analysis System ssnrnrnvrrrrvrrveverererererenrsrrrrrnrrsnrssereveveverererennnn 237 HAI 118 Data Acquisition amp Analysis System 238 240 Vibration Isolation BenchMate Series Vibration Free Platforms 241 Vibralite BreadboardS EE 242 Series Labmate Table 243
17. Glass Seal Compression Caps E W4 64 1293 2 QC 10 Compression cap for 1 0 mm glass holders W4 64 1294 2 QC 12 Compression cap for 1 2 mm glass holders W4 64 1295 2 QC 15 Compression cap for 1 5 mm glass holders QC xx E W4 64 1375 2 QC 17 Compression cap for 1 7 mm glass holders S W4 64 1296 2 QC 20 Compression cap for 2 0 mm glass holders 2 Connector Pins and Jacks W4 64 1283 3 HC 10M 1 mm pin for Q and PE holders with A or T connectors W4 64 1284 3 HC 13M 1 3 mm pin for Q and PE holders with B co BNC connector W4 64 1285 3 HC 20M 2 mm threaded pin for E ME and theta holders ge W4 64 1286 3 HC 21F 2 mm jack assembly for all series holders W4 64 1287 3 HC 22M 2 mm pin for Q and PE holders with M connector Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 323 Premium Capillary Glass Warner capillary glass ts known worldwide for its consistent high quality e Ends are fire polished to prevent damage to the Premium Standard Wall Tubing is available in two wall thickness standard wall and thin wall Additionally a variety of diameters is offered to cover most needs of micropipette and microelectrode research ll rubber gaskets when inserted into electrode Borosilicate holders e Glass is cleaned with deionized water before OD ID Length being packed in dust free containers Order Model mm mm mm Oty Pkg W4 64 0765 G100 3 1 0 0 58 75 500 W4 64 0766 G
18. PESOW Series PE30W Series Electrode Holders for Patch Perfusion Specifications Body Material Polycarbonate Body Style 30 Connectors 1 mm Pin 1 mm Pin Threaded and BNC Coupling Ag Wire PE30W BxxP Port 2 mm diameter Polycarbonate Perfusion Port port accepts tubing up to I mm diameter Standard Glass Sizes 1 0 1 2 1 5 1 7 and 2 0 mm Patch Clamp Compatibility Connector For use with PE30W TxxP 1 mm Pin Warner Patch Clamp Models PC 501 amp PC 505 Axon Patch Clamps prior to 2 1 95 e Microperfusion perfusion at the pipette tip BNC List Heka and Dagan Patch Clamps Ge 2 1 mm Pin with Axon Patch Clamps and Microelectrode For microperfusion inside the pipette threaded collar Amps prior to 2 1 95 e Perfusion at the electrode tip Is possible with the PE Series holders REG E They include an additional port in line with the glass bore allowing for ENEE SE Se wi 3 dE 1 Nehr E and Eckert R Fast patch pipette internal perfusion with minimum solution Ko the insertion of microbore tubing into the pipette this port includes a flow Grinnel A D Armstrong D and Jackson M B Calcium and lon Channel S threaded cap and seal for up to 1 mm diameter tubing See the reports Modulation Plenum Press N Y Cull Candy S G Miledi R and Parker I Single D f d below torini ti eini siontan glutamate activated channels recorded from locust muscle fibers with perfused referenced below for information on the microperfu
19. Pipette Pullers A state of the art microprocessor controlled horizontal pipette puller for pulling single or multi barrel micropipettes W4 69 0151 e Two models available Single barrel Multi barrel e Microprocessor controlled system performs programmable multiple step pulling sequences e Produces micropipettes for patch clamping intracellular electrodes injection micropipettes micro needles etc e Consistent pulling force by utilizing pneumatic pressure instead of gravity or magnetic fields The PMP 102 is a state of the art microprocessor controlled horizontal pipette puller that has been designed to pull two Identical single barrel pipettes at the same time The pipettes can be used as patch clamp electrodes intracellular electrodes injection micropipette or micro needles The PMP 107 is similar to the PMP 102 but is designed to pull a single 4 or 7 barrel pipette Under program control the PMP 107 can automatically heat twist and pull a multi barrel pipette There is no need for any manual rotation or any inconsistent timing interrupt control Exclusive Optical Digital Taper Measurement Instead of a mechanical tip length setting as on other pipette pullers the PMP 102 and PMP 107 use an exclusive optical digital ruler to perform precise taper length setting real time measuring and controlling With this feature a user can handle the taper pulling of pipette tips precisely and very easily Equipped with a
20. Solution Heaters csscsssssssscsssesssssecessrsssssecseersrsrseesseeararsees 117 120 Other Cooler Warmers imrsrnravnrsvnnrnrnvnrnvnvnnrnrnrnenenvnesnsarnenenennsesnsnrnenenennsn 121 122 Syringe Warnes ergeet gedeien 123 126 Accessories for Temperature Control Products seess 127 129 Micro Incubation Section Okolab Microscope Incubators Okolab Microscope Incubators LL marvmassessenmeadanrammmnmnmmnei 132 141 Cryo Water Jacketed CO Microscope Stage Jncubator eee 142 147 CO Microscope JJ E 148 158 Okolab Gas Mixers Two Gas Mixer 20F E 159 Digital Gas Mixer DGTCO2BX qccccccisencssctccenedasendessselevemeasdedeecdacviecenscelinansiess 160 161 Microincubators Culture Dish Heater DRS enetiereetet ed cavestcteciectcesblad deaaideriatwacedeedeotbeseiiead 162 Microincubator System for 35mm Cell Culture Dishes DH 251 163 Microincubator System DH A0 164 Model TB 3 CCD Thermal Insert for Prior Nano Z Stage sosovrvrvrvrvrvrrrrrrrrerrrrnrnrnrnnn 165 Open Perfusion Microincubator HIM 166 Patch Slice Microincubator EE 167 Model TB 3 CS Thermal Insert for Prior Nano Z Stage nsrrrvrvrvrrrvevrverrrrrererrrrnrnnnnnnn 168 Heated Platform for Chambered Slides LCHI 169 Microscope Stage Incubator LML 170 Accessories for PDMI 2 PSMI CSMI amp Leiden Chambers ooovrnrrrrrrrrrrrrrrererernen 171 Culture Dish Platforms senrnvnrnononenvnvnonenvnvnvnenenenvnvnenenenvnenenenenenenenenenen
21. a good option My injection pipettes rarely clog Easy to Use Inject with the touch of a button or a tap of the foot switch it s as easy as that Reproducible Pressure to the pipette is controlled precisely through a multi turn regulator and is reported digitally for easy repeat Injection time is digitally controlled in 10 msec steps between 0 01 to 0 99 seconds and in 1 second steps between 1 to 99 seconds Pipette tip diameter and hence delivered volume are easy determinations once the timing and pressure are known Versatile Deliver volumes from femtoliters to microliters with the same instrument resulting in a wide range of applications Pressure Capabilities The PLI 100 features two negative and three positive pressure pneumatic capabilities The negative or vacuum functions allow the user to 1 Fill micropipettes from their tips reducing wastage of valuable injectables 2 Provide a means to secure and manipulate a cell using a holding pipette Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com PLI 100 Medical Systems Pico Injector continued The positive pressures allow the user to 1 Eject precise amounts of fluids 2 Create a balance pressure which prevents backflow into the micropipette following an injection 3 Clear a micropipette of material in it Unique Features The PLI 100 important Balance Hold
22. also need to be of the highest quality and provide the necessary reliability accuracy and repeatability to insure proper results Harvard Apparatus Inc manufactures and sells the complete range of product that are needed to successfully microinject and or microperfuse Pipette Pullers pages 331 to 334 Micromanipulators pages 295 to 304 274 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com NEW Low Cost Pico Injector PICO LITER INJEC AP wal WPL Dk SS aT BT TOM He e Femtoliter to microliter injections Digital readouts for injection pressure time and count e Reliable optically encoded circuit for injection time set e KI Easy to use The PLI 10 Pico Injector reliably delivers ejections from femtoliters to nanoliters through micropipettes by applying a regulated pressure for a digitally set period of time Pressure to the pipette is controlled precisely through a multi turn regulator and is reported digitally for reproducibility Injection time Is set using an optically encoded circuit which permits fine and coarse settings from a single knob The system timer can be controlled in three ways front panel push button foot switch and external trigger input BNC Typical applications are large volume injections large volume is considered to be in the 10 100 Pico liter range extracellular sample delivery and Xenopus Qocyte injecti
23. and regulation 0 to 30 p s i optional O to 10 or 0 to 90 p s i as well as precise timing The PPM 2 Module is comprised of a precise pressure regulator digital display transducer and a timing mode switch It connects to an external pressure source such as a compressed bottle of N2 which can be set to provide continuous or periodic pressure pulses ranging from 0 to 30 p s i Outputs include 0 to 1 volt proportional to output pressure as well as a sync pulse coincident with pressure cycle Operating Modes Ejection Timing and Mode Switch The mode switch provides five push button controls which include operations such as cycle trigger gate continuous and termination Cycle Mode In the cycle mode after selecting single or recycle operation the start push button will initiate the ejection pumping action In this mode each succeeding pump module is automatically triggered after the pause time of the preceding event has been completed Both eject and pause times can be preset to cover a range from 30 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second resolution Trigger Mode When the TRIG switch is energized the eject time interval will be started by virtue of an externally applied trigger pulse fed to the respective inputs on the CM I control panel Eject timing intervals can be preset to cover a range from 30 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a
24. axis vertical gives 3 mm 0 12 in of movement per revolution e Lever tips the tool quickly for such actions as hanging a petri dish and the preset stop returns tool tip to focus plane e Stop screw prevents damaging probe tip e Probe holder tilts to 90 e Available for right or left handed use With this very sensitive mechanical joystick micromanipulator motion even in the micron range Is possible This micromanipulator is so stable and strong that it can accommodate even piezo steppers Joystick micromanipulators provide movement of probes that are direct reductions of the speed and direction of the hand movement Under the microscope the probe appears to move directly with the hand Typical applications for the Joystick micromanipulator are positioning of holding pipettes to grab and hold freely mobile cells or the manipulation of larger cells The joystick drives the probe in the X probe and Y horizontal directions The reduction of the joystick travel relative to the hand may be adjusted from 1 15 to 1 150 The travel of the joystick ranges from 0 35 to 4 mm 0 014 to 0 16 in depending on the reduction gear ratio being employed The Z axis also has a fine movement with a reduction gear ratio of 1 10 relative to the coarse movement This fine movement is operated by a further knob on the Z axis providing 3 mm 0 12 in of movement per Wat Phone 203 776 0664 Toll Free U S revolution The Z axis has a lever on the
25. coarse adjust which enables the operator to lift the tool tip quickly for such operations as changing a petri dish A preset stop ensures that the tool tip will return to the previous focusing plane when again lowered The stop screw prevents the probe from being inadvertently broken by being driven into the slide or dish The stop screw also prevents downward drift of the micromanipulator so that the pipette or electrode may be left in a stable position over a long period of time The probe holder may be tilted up to 90 This joystick micromanipulator is offered in right or left handed versions It is also offered with a mounting clamp for a 13 mm in OD vertical rod or with a wide table clamp when particularly stable mounting Is required Joystick Micromanipulator Coarse Positioning Axis Travel X probe 37 mm 1 45 in Y horizontal 20 mm 0 79 in Z vertical 25 mm 1 in Order Product W4 60 0582 Right Handed Joystick Micromanipulator with Mounting Clamp for 13 mm 0 5 in Rod W4 60 0583 Left Handed Joystick Micromanipulator with Mounting Clamp for 13 mm 0 5 in Rod W4 60 0604 Tool Holder pkg of 3 W4 69 1066 10mm Rod Clamp for use with Magnetic Bases W4 69 1067 12mm Rod Clamp for use with Magnetic Bases Ultraprecise Micromanipulators For accurate reproducable and precise positioning These ultraprecise micromanipulators have the mass 6 kg 13 2 Ib and solid workmanship to support the most precise movemen
26. displayed on the digital display of each corresponding Pump Module This input can be connected to a computer D A converter when external programming is desired Analog Output The analog output terminal provides a buffered voltage which is proportional in magnitude and polarity to the actual current passed from the current pump into the pipette The conversion ratio is 5 mV nA This output can be polygraphically recorded to monitor progress of the experiment Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Sync Output The sync output provides a TTL pulse that coincides with the eject time This output is provided to trigger external devices such as a computer event counter etc Specifications Current Pump Ejection Current Ejection Indicator Retention Current Analog Input Analog Output Sync Output Current and Resistance Resistance Current Switch Current Mode Resistance Mode Compliance Exceeded Indicator Voltage Readout Switch Order W4 65 0603 Product IP 2 lontophoresis Pump Module Compliance 105 V linear constant current source Pulsing controlled by Ejection Timing Mode Switch amplitude adjustable by 10 turn ejection control and range switch from 0 to 50 or 0 to 500 nA polarity is selected by polarity switch accuracy of I nA Red LED lamp indicates Eject time period green LED lamp indicates pause time period Am
27. glass from Clark was introduced in 1997 as a substitute i E for the Corning 7052 glass a favorite amongst researchers performing EE 2 5 patch clamping Initial tests showed the 8520 glass to be equal to the i i e 7052 in noise performance More importantly seals were formed faster Premium Corning 7056 Patch Glass and maintained for longer periods op ID Length S Order Model mm mm mm Oty Specifications W4 64 0813 G75150T 4 1 50 1 10 100 225 Composition gt 10 Si0 gt 10 B 0 gt 1 Al 0 gt 1 K 0 lt 1 Na 0 W4 64 0814 G751657T 4 1 65 1 20 100 190 lt 1 D lt 1 ZnO lt 1 As 0 lt 1 TiQ lt 1 ZrO Softening Temperature 720 C Premium Corning 8161 Potash Rubium Lead Patch Glass O lt Dielectric Constant 4 6 Premium Custom 8520 Patch Glass OD ID Length Order Model mm mm mm Qty Pkg W4 64 0817 G85150T 3 1 50 1 16 75 225 W4 64 0818 G85150T 4 1 50 1 16 100 225 W4 64 0819 G85165T 3 1 65 1 28 75 190 This glass pulls at a lower temperature and is easily shaped It is W4 64 0820 G85165T 4 165 1 28 100 190 recommended for low noise recordings However the lead content should TB considered for the intended application Clark Custom 8520 Patch Glass OD ID Length Specifications Order mm mm mm Oty Composition 51 PbO 39 Si0 6 K 0 2 BaO W4 30 0091 12 093 7 30 Softening Temperature 600 C Wa 30 0089 1 2 0 93 100 350 Dielectric Constant 8 3 T E a Premium Corning 8161 Potash Ru
28. mm Rod Clamp o se z z e Magnetic Bases Base Main Pole 1st Sub Pole 2nd Sub Pole Order Width Length Height Diameter Length Diameter Length Diameter Length Hold Force W4 69 0225 50mm 58 mm 55 mm 12 mm 176 mm 10 mm 165mm 800 N W4 69 0226 50mm 58 mm 55 mm 12 mm 194 mm 10 mm 165mm 800 N W4 69 0227 50mm 58 mm 55 mm 14 mm 178 mm 12 mm 165mm 800 N W4 69 0229 50mm 73 mm 55 mm 20 mm 178 mm 14 mm 165mm 12mm 130 mm 1000 N W4 69 0230 50mm 58 mm 55 mm 16 mm 315 mm flex with holder for 8 mm rod 800 N Magnetic Holders Holder Tapped Hole Mounting Screw Holes Order Width Length Height Diameter Thread Depth Qty Diameter Depth Span Hold Force W4 69 0231 50 mm 58 mm 55 mm M8 1 25 7 800 N W4 69 0232 45 mm 45 mm 20 mm M8 1 25 6 4 M6 6 25 65 N W4 69 0233 65 mm 65 mm 20 mm M8 1 25 6 8 M4 6 25 200 N W4 69 0234 90 mm 90 mm 20 mm M8 1 25 6 8 M4 6 25 250 N W4 69 0237 50 mm 73 mm 55 mm M8 1 25 7 1000 N 304 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com ESW MxxP E45 MxxP AFEIANE A T Es IS d Be U lo 4L ANALIIT et LL 212 i NItN IV H 26 h OIC er VVICII IVII 149 IODIUE I E Series Applications include intracellular and extracellular recording lontophoresis and ion specific measurements They are available with choice of body style and electrical coupling and may be specified with or without a port or
29. of the compressor Furthermore the internal motor is mounted in a closed motor house reducing the noise level even further Due to the design of the motor the oil is also used for cooling the motor The motor is mounted on springs inside the motor housing which means that hardly any vibrations are imparted to the surroundings Two noise reduction chambers on the intake side and two noise reduction chambers on the pressure side ensure absorption of the noise The compressor Is also supplied with rubber feet preventing vibrations from imparting to the mounting bolts and foundation The noise level of the oil lubricated compressor is 45 dB A below the noise level of a refrigerator The oil minimizes the wear and tear of the vital parts in the compressor prolonging the lifetime and at the same time ensuring low maintenance costs Specifications Input Voltage Range Maximum Current Motor Displacement FAD 8 bar Max Pressure Tank Size Noise Level Physical Dimensions Size HxWxD Weight Warranty Order Model W4 64 1701 AIR 1 U W4 64 1702 AIR 2 120 or 230 VAC specify at time or order 6 2AQ 120 VAC 2 9A 230 VAC 0 54 HP 0 40kW 60 l min or 2 12 CFM 32 l min or 1 13 CFM 8 bar 120 psi 4 liters 1 1 gallon 45 dB A 382 x 300 x 334 mm 15 x 11 8 x 13 2 in 23 kg 50 6 Ib One year parts amp labor Product Ultra low noise 120VAC Ultra low noise 230VAC microinjection gt D i an T
30. positioning and long life The lateral Y axis and vertical Z axis planes each have 20 mm of travel graduated in 0 1 mm increments The probe direction X axis has 37 mm ts rigidity and scope make It ideal for any application For repetitive work where a number of stations may be required the Standard Manual Control Micromanipulator with its numerous options generally meets any requirement To hold freely mobile cells or manipulate larger cells the Micromanipulator with Mechanical Joystick see page 300 is flexible yet stable of travel Two adjustments are provided The coarse adjustment is graduated in 0 1 mm increments while the fine adjustment provides 10 mm of travel with precision positioning graduated in 0 01 mm increments A unique and valuable feature of these standard manual control micromanipulators is that the three control knobs are located in a single plane This positioning permits one handed control of a single instrument without taking the operator s eyes from the microscope It also allows several micromanipulators to be closely positioned side by side with all control knobs easily accessible They are available for both right and left handed use Tool Electrode Holder Each micromanipulator is supplied complete with one or two tool electrode holders The second holder has two fine controls that allow movement of 8 mm in both the lateral Y and probe X direction independent of the micromanipulator Two additi
31. powerful computerized tip sensing function the PMP 102 and PMP 107 can automatically finish the tip pulling Computerized Heater Control The PMP 102 and PMP 107 use an advanced microcontroller design to perform real time heater monitoring and pulling control Thus they will always provide precise heating power despite multiple consecutive pipette pullings or changes in thermal electrical characteristics Programmable Sequences for Creation and Reproduction There are 25 user programmable pulling sequences with 18 steps in each sequence Users can start the pulling sequence by just pressing the Start button Factory Installed Preset Programs for Major Different Pipette Pulls Every pipette puller is pre installed with a number of different pipette pulling programs With just a few modifications to these steps or parameters new programs can be configured Pneumatic Pulling Force Both pullers use a precise controlled pneumatic pressure as the pulling force rather than gravity or magnetic fields used by other manufacturers This technique provides more controlled even and consistent dragging characteristics allowing the units to pull an ideal injection tip or microelectrode tip A precision micro linear ball bearing rail and advanced pneumatic components are used to provide no fault pulling movement Programming A simple 4x4 keypad and full information display allows the user to easily control and read all pulling parameters Progr
32. their pharmacological studies of drug evoked responses such as neurosynaptic discharges contraction and changes in chemical concentration What emerged was a reliable accurate easy to use Neuro Phore BH 2 system that is capable of accommodating high impedance multi barreled micro electrode pipettes Digitally controlled eject and pause timing utilizes easy to read and set digital panel switches on each iontophoretic or pressure module channel Unbalance and out of compliance indicators are a great aid in troubleshooting clogged pipettes and other problems uondafuio1du External analog input control allows external programming of complex or closed loop injection protocols Current and electrode resistance readout with large bright easy to read digital displays o se e L System Configuration Balance Module Oo a The Neuro Phore BH 2 System is modular and is comprised of the following components all ordered separately e Used by leading researchers for over 20 years One BH 2 Mainframe Chassis W4 65 0600 that can accommodate 1 to e Modular 5 lontophoretic Pump and or Pressure Pump Modules Mainframe e Minimal noise Chassis can be rack mounted or set on table e Up to 6 channels One BM 2 Control and Balance Module W4 65 0602 e Digitally controlled eject and pause timing P 2 lontophoretic Pump Modules W4 65 0603 and or e Mix and match pump types PPM 2 Pressure Pump Modules W4 65 0604 One l
33. to the sub picoliter liter range into or around various types and sizes of cells with accuracy to 0 1 micron The process of extruding substances through these micropipettes is accomplished through the use of either direct Harvard Apparatus Medical Systems Picoliter Injectors pages 275 to 282 hydrostatic pressure Pressure Injection or by moving charged ions that are the result of an applied electric field lontophoresis without the use of hydraulic flow Applications of microinjection range from assisted In vitro cell fertilization techniques to the transport of molecular and cellular elements These substances are typically injected into the cell to manipulate and or monitor the fundamental biochemistry of a specific living cell Substance that can be injected include cellular organelles kinases histochemical markers such as horseradish peroxidase or lucifer yellow proteins metabolites microbeads ions antibodies genes molecular biology mRNA and DNA etc The precise delivery microperfusion of small volumes picoliter to milliliter of various agents and drugs to a cell or group of cells for applications such as pharmacological drug testing can also be accomplished using these techniques uondafurosdnu Abiojolg 1199 In order for the researcher to conduct any of the above experiments and get meaningful results the tools equipment used in these experiments must provide not only the specific functionality but they
34. vent see accompanying chart The holders are used with microelectrode amplifiers that have either a 2 mm pin or 2 mm jack headstage connector Amplifier headstages with the BNC connector will use a Q Series holder ERW FxxN E45 FxxN ERP MxxP Specifications Body Material Body Styles Connectors Coupling Port Vent Standard Glass Sizes Acrylic 6 3 mm diameter Straight 45 and 90 2 mm Pin or 2 mm Jack Ag Wire or Ag AgCI Pellet 2 mm diameter Polycarbonate 0 8 mm diameter Hole 1 0 1 2 1 5 and 2 0 mm Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 305 SJAp OY 3P01 23J30131W o se z e lt 306 E Series E Series Straight Body Holders E SERIES Straight Body Holders Order W4 64 0992 W4 64 0993 W4 64 0994 W4 64 1260 W4 64 0995 W4 64 0996 W4 64 0997 W4 64 0998 W4 64 0980 W4 64 0981 W4 64 0982 W4 64 0983 W4 64 0984 W4 64 0985 W4 64 0986 W4 64 0987 W4 64 1011 W4 64 1012 W4 64 1013 W4 64 1014 W4 64 1015 W4 64 1016 W4 64 1017 W4 64 1018 Wire Pellet Connector Pellet Wire 2mm Pin 2 mm Jack 2mm Pin Port No Yes Yes No Glass OD mm 1 0 1 2 1 5 2 0 1 0 1 2 L 2 0 1 0 1 2 15 2 0 1 0 1 2 15 2 0 1 0 1 2 L5 2 0 1 0 1 2 1 5 2 0 Model ESP M10N ESP M12N ESP M15N ESP M20N ESP M10P ESP M12P ESP M15P ESP M20P ESP F10
35. with membrane buttons is now separated from the slicer module for improved ergonomics and protection of electronic components 3 axis adjustable specimen mounting pedestal Easy to release slicer head for safety and ease of specimen mounting Slice thickness is now adjustable from 1 999 microns in 1 micron increments One touch home button to return the blade arm to a predetermined point near the specimen The specimen tray is made from clear polycarbonate for improved viewing of the slice operation The pivot pins which come as a standard on the pedestal as well as the vice holder allow changing of the angle of the pedestal relative to the blade Easy sample orientation in all directions The tray includes a lock on feature for a more secure mount to the platform The standard unit comes complete with our unique pivotal tissue pedestal you can mount the specimen directly on the pedestal without the need for vices or blocks mounted in the sample tray Automatic Oscillating Tissue Slicer OTS 5000 The OTS 5000 Oscillating Tissue Slicer represents the most thoughtfully designed solution to sectioning either fixed or fresh tissue and can prepare sections as thin as 5 microns Embedding or freezing of the sample is not required thus tissue preparation time is reduced dramatically The risk of distortion and artifacts normally associated with these procedures are eliminated The OTS 5000 meets the highest standards fo
36. www harvardapparatus co uk UNITED STATES Coulbourn Instruments Attn Sales Department 5583 Roosevelt Street Whitehall Pennsylvania 18052 USA e mail sales coulbourn com website www coulbourn com Authorized Distributors The following Distributors are available to serve customers outside of the US They can provide technical assistance catalog information and quotations including shipping and importation costs and after sales service ARGENTINA ETC Internacional S A Allende 3274 C1417BMV Ciudad Aut noma de Buenos Aires Argentina phone 54 11 4639 3488 fax 54 11 4639 6771 e mail etcventa etcint com ar danielr etcint com ar pagina web www etcint com ar AUSTRALIA SDR Clinical Technology 213 Eastern Valley Way Middle Cove NSW 2068 Australia phone 61 02 9958 2688 fax 61 02 9958 2655 e mail sdr sdr com au website www sdr com au BELGIUM nh FRENCH SWITZERLAND Harvard Apparatus S A R L 6 Ave des Andes Miniparc Bat 8 91952 Les Ulies Cedex phone 33 1 64 46 00 85 fax 33 1 64 46 94 38 e mail info harcardapparatus fr BRAZIL Sellex Inc R Cardoso de Almedia 788 114 S 1 05013 001 Sao Paulo SP Brazil phone 55 11 3872 2015 fax 55 11 3872 1024 e mail vendas sellex com website www sellex com CHILE Del Carpio Analisisy Asesorias Ltda Avda Sucre 2596 Nunoa Santiago Chile phone 56 2 269 1348 fax 56 2 341 5397 e mail cgarcia delcarpio cl
37. 0 78 15 500 Order mn mm mm mm Oty W4 30 0038 1 0 0 78 100 500 W4 30 0116 15 023 0 17 75 100 W4 30 0039 1 0 0 78 150 500 A W430 0114 15 023 0 17 100 100 W4 30 0052 1 2 0 94 15 350 W4 30 0115 15 023 0 17 150 100 W4 30 0050 1 2 0 94 100 350 W4 30 0119 20 0 30 0 22 75 100 Wa 30 0051 1 2 0 94 150 350 B W430 0117 20 030 022 100 100 W4 30 0068 1 5 1 17 15 225 W4 30 0118 20 030 0 22 150 100 W4 30 0066 1 5 1 17 100 225 A 0 435 mm HRC W4 30 0067 1 5 1 17 150 225 B 0 59 mm OD 2 x Wall Sept W4 30 0128 2 0 1 56 75 125 W4 30 0077 20 1 56 100 125 Half Channel W4 30 0078 2 0 1 56 150 125 Radius A or B 328 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Clark Capillary Glass continued Borosilicate Multi Barrel e High quality borosilicate glass e Two three five and seven barrel configurations with filament e Capillaries fused along their full length Borosilicate Multi Barrel OD Wall of Order mm mm Septum Barrels Qty W4 30 0003 1 5 0 86 75 mm 2 100 W4 30 0001 1 5 0 86 100 mm 2 100 W4 30 0002 1 5 0 86 150 mm 2 100 W4 30 0007 1 2 0 69 100 mm 3 100 W4 30 0008 1 2 0 69 150 mm 3 100 W4 30 0012 1 2 0 69 100 mm 5 65 W4 30 0013 1 2 0 69 150 mm 5 65 W4 30 0014 1 0 0 58 100 mm 60 W4 30 0015 1 0 0 58 150 mm 7 60 Aluminosilicate Capillaries with Filament luminosiiicate Capillaries In recent years there has been a developing interest in fabricating mic
38. 0746 W4 64 1112 W4 64 0825 W4 64 0826 W4 64 0827 W4 64 0979 W4 64 0828 W4 64 0845 W4 64 0846 W4 64 0847 W4 64 1099 W4 64 0848 W4 64 1059 W4 64 1060 W4 64 1061 W4 64 0969 W4 64 1062 Wire Pellet Connector Wire Straight 45 Port No Yes No Yes Glass OD mm 1 0 1 2 L 1 7 2 0 1 0 1 2 1 5 1 7 2 0 1 0 1 2 1 5 1 7 2 0 1 0 1 2 1 5 1 7 2 0 Model QSW B10N QSW B12N QSW B15N QSW B17N QSW B20N QSW B10P QSW B12P QSW B15P QSW B17P QSW B20P Q45W B10N Q45W B12N Q45W B15N Q45W B17N Q45W B20N Q45W B10P Q45W B12P Q45W B15P Q45W B17P Q45W B20P Ger DEN Ca CA g C port Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Q Series Q Series Holders with BNC Connector continued Q SERIES Holders with BNC Connector Glass Order Wire Pellet Connector Port OD mm Model W4 64 0865 90 No 1 0 QRW B10N W4 64 0866 1 2 QRW B12N GO CG W4 64 0867 1 5 ORW B15N W4 64 1104 17 QRW B17N W4 64 0868 2 0 QRW B20N W4 64 1079 Yes 1 0 QRW B10P W4 64 1080 1 2 QRW B12P GO W4 64 1081 15 QRW B15P sot W4 64 0974 17 QRW B17P W4 64 1082 2 0 QRW B20P W4 64 1357 Hybrid Straight No 1 5 QSH B15N Bess W4 64 1358 17 OSH B17N GET W4 64 1359 2 0 QSH B20N W4 64 1360 Yes 15 QSH B15P 8 W4 64 1361 1 7 QSH B17P W4 64 1362 2 0 QSH B20P port E W4 64
39. 0833 Straight Yes 1 0 QSW M10P et 2 W4 64 0834 Straight Yes 1 2 OSW M12P AB E W4 64 0835 Straight Yes 15 OSW M15P E W4 64 0836 Straight Yes 2 0 OSW M20P S Compatible with Axon 2 gt D a an T 4 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 315 SJAp OY 3P01 23J30131W o sch z 2 lt 316 ME Series ME Series Electrode Holders Microelectrode holders for every application and manufacturer Intracellular and Extracellular Recording e Microiontophoresis Pressure Ports A Accepts 1 5 mm tubing B Accepts 2 4 mm tubing ML Accepts female Luer fitting FL Accepts male Luer fitting T Accepts 7 28 male thread fitting Holders with the T port are compatible with the Picospritzer system made by General Valve Corp Specifications Body Material 9 3 mm diameter acrylic Body Style Straight Connector 2 mm jack Coupling Ag AgCI pellet or Ag Wire Standard Glass Sizes Handle 1 0 1 2 1 5 and 2 0 mm holder ME SERIES Holders Order W4 64 1220 W4 64 1221 W4 64 1222 W4 64 1223 W4 64 1224 W4 64 1225 W4 64 1226 W4 64 1227 W4 64 1232 W4 64 1233 W4 64 1234 W4 64 1235 W4 64 1228 W4 64 1229 W4 64 1230 W4 64 1231 W4 64 1236 W4 64 1237 W4 64 1238 W4 64 1239 W4 64 1180 W4 64 1181 W4 64 1182 W4 64 1183 Wire Pellet Wire Body Style Pellet Port 2mm Straight Jack 2mm S
40. 1 second resolution Gate Mode When the GATE switch is energized pumping action will be started by virtue of a gate input signal applied to the respective trigger gate terminals on the CM I control panel The eject and pause time settings are Fully pulled Micron tip e Easy filling e Minimal tip resistance e Minimal leakage e Available pre pulled or fully pulled not operative in this mode since the eject time function is slaved to the duration of the gate input Continuous Mode Eject pump action is continuously maintained when CONT switch is energized Termination Mode When the TERM switch is energized the output pressure Is automatically diverted from the preparation This function is particularly useful for setting up the desired pressure range and timing while preventing ejection of any drug from the pipette Specifications Source Gas Input Pressure Input Filter Output Pressure Output Pressure Display Pressure Pulse Width Minimum Maximum Gas Input and Output Couplings Analog Output Sync Output Eject Time Indicator Pause Time Indicator Order Product Air or Nitrogen recommended no explosive or combustible gases 125 p s i g 7 8 kg cm2 maximum 5 um element 0 to 99 9 p s i g 0 to 7 kg cm2 30 p s i standard Three decimal digits 30 msec 99 sec 990 sec optional Quick disconnect type Lemo miniature connector voltage proportional to output pressure 0 to 999
41. 100 4 1 0 0 58 100 500 Warner capillary glass is known worldwide for its consistent high W4 64 0770 G120 6 12 0 69 150 350 quality The glass is precision drawn to insure reliability and W4 64 0771 G150 3 1 5 0 86 75 225 consistency from batch to batch The full line of glass capillaries W4 64 0772 G150 4 1 5 0 86 100 225 listed here is stocked for fast shipment W4 64 0773 G150 6 1 5 0 86 150 295 Standard Wall Thin Wall W4 64 0774 G200 3 20 1 16 75 125 Premium Thin Wall Borosilicate We i i OD ID Length Capillaries with Filament Order Model mm mm mm _ Oty Pkg m A small diameter filament is fused to the glass inside diameter to W4 64 0778 G1001 4 10 0 78 100 500 2 facilitate rapid solution filling through capillary attraction This is W4 64 0780 G120T 3 1 2 0 94 75 350 especially important for the very small diameter of sharp electrodes W4 64 0781 G120T 4 1 2 0 94 100 350 typically used for intracellular studies and microiontophoresis Wa 64 0783 G150T 3 15 1 17 75 225 D a Premium Corning Type 7740 Pyrex W4 64 0784 G150T 4 1 5 1 17 100 225 G W4 64 0785 G150T6 15 1 17 150 225 Corning 7740 Borosilicate is the most commonly used glass in electrode fabrication because of its mechanical strength chemical o n durability electrical resistivity and its ability to withstand thermal Premium Standard Wall 6 gt stress It is also easy to work with and suitable for a wide range of Borosilicate with Filament ZS applications Corn
42. 10P W4 64 0838 1 2 QOSW T12P W4 64 0839 15 QSW T15P W4 64 1097 1 7 QSW T17P W4 64 0840 2 0 QSW T20P W4 64 0857 45 No 1 0 Q45W T10N W4 64 0858 1 2 Q45W T12N W4 64 0859 1 5 Q45W T15N W4 64 1102 1 7 Q45W T17N W4 64 0860 2 0 Q45W T20N W4 64 1071 Yes 1 0 Q45W T10P W4 64 1072 1 2 Q45W T12P W4 64 1073 1 5 Q45W T15P W4 64 0972 1 7 Q45W T17P W4 64 1074 2 0 Q45W T20P W4 64 0877 90 No 1 0 QRW T10N W4 64 0878 1 2 QRW T12N W4 64 0879 1 5 QRW T15N W4 64 0744 1 7 QRW T17N W4 64 0880 2 0 QRW T20N W4 64 1091 Yes 1 0 QRW T10P W4 64 1092 1 2 QRW T12P W4 64 1093 1 5 QRW T15P W4 64 0977 1 7 QRW T17P W4 64 1094 2 0 QRW T20P W4 64 1363 Hybrid Straight No 1 5 QSH T15N W4 64 1364 1 7 QSH T17N W4 64 1365 2 0 QSH T20N W4 64 1366 Yes 1 5 QSH T15P W4 64 1367 1 7 QSH T17P W4 64 1368 2 0 QSH T20P S gt mn o ES microelectrode holders Cell Biology port s 8 WEE HP 205 W4 64 1288 with Teflon Sleeve Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 313 SJAp OY 3P01 23J30121W o se z e 314 O Series Q Series Holders with BNC Connector Q45 BxxP Compatible with Heka List and Dagan Patch Clamps NOTE Holders without ports are not suitable for patch clamp recording Q SERIES Holders with BNC Connector Order W4 64 1109 W4 64 1110 W4 64 1111 W4 64
43. 18 Pipette Sterilization and Storage Container for 2 0 mm Pipettes Designed for iontophoretic applications and pneumatic ejection of drugs through up to seven barrels Pre pulled Blank Fully pulled Micron tip 7 Barrel lontophoresis Micropipette e Easy filling e Minimal tip resistance e Minimal leakage e Available pre pulled or fully pulled The MS 7P Micropipette has been designed for iontophoretic use as well as for pneumatic ejection of drugs The standard blank consists of seven barrel pyrex Omegadot tubing 1 5 mm outer diameter and 1 2 mm inner diameter pre pulled to 2 0 mm formation as shown The design of the micropipette readily lends itself to electrical connection by inserting Ag wire or to pressure tube attachment for pneumatic use The overall length of the blank assembly is 10 centimeters The micropipette is supplied as either pre pulled blanks or fully pulled to a micron tip as shown The micron tip is not bumped and therefore can be finalized prior to use Features The orifice ratio of each barrel is 0 80 and thus is optimized for minimal tip resistance when pulled to micron size Qmegadot tubing permits easy filling Fanned out outer barrel assembly provides separation thus avoiding leakage due to spills and any cross coupling between barrels The 22 mm long twisted segment of the blank assembly assures singular tip formation during pulling action thus provides flexibility in a selection of a desi
44. 2 Pneumatic QH 01 and IH 01 PPS 2 System Can Pressure Modules Order W4 65 0604 which are ordered separately Support 1 to 4 Modules W4 65 0605 DP 1 Dummy Module to Maintain Timing The Control Module CM 1 includes a time clock Panel mounted push PPS 2 System Can Support 1 to 4 Modules buttons as well as TTL electrical inputs are provided to control the following functions Cycle Start Cycle Stop Trigger each PPM 2 and Gate each PPM 2 In essence the CM 1 Control Module provides all necessary signals to operate the sequential and single shot timing for the W4 65 0210 Model SC 01 Tygon Flexible Hose Micropipette Interface Coupling for Pneumatic PPM 2 Use W4 65 0211 Model SC 02 Tygon Flexible Hose Micropipette Interface Coupling for Current IP 2 Pneumatic installed PPM 2 Pneumatic Pump Modules PPM 2 Use A Self Cycle mode control switch controls independent self timing action of W4 65 0212 Model IH 01 Input Hose 2 7 m 9 ft Coupling each of the pneumatic pump modules The self timing action permits each to PPM 2 PPM 2 Pump Module to eject and pause for a predetermined period W4 65 0213 Model OH 01 Output Hose 2 7 m Actuating any of the self cycle control switches causes the respective PPM 9 ft Including SC 01 Coupling 2 Module to be engaged into the self timing mode The push buttons at the W4 65 0214 Model OH 02 Output Hose 2 7 m corresponding trigger gate terminals initiate the self cycle timing action 9 ft Incl
45. 20 VAC 50 Hz Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Microdrive 864 1 and 864 2 Hands off control for precise positioning HSE HA Microdrive 864 1 e Resolution down to 2 um The motorized micrometer drive Type 864 1 is based on the motor X axis of the long established micromanipulator DC 3K Together with the HSE HA Microdrive Controller Type 864 it permits remotely controlled displacement of microelectrodes or capillaries e g in intracellular potential recording patch clamp experiments or stereotaxic investigations in the brain It thus represents a complete alternative to hydraulic micro drives which have now become very expensive The mechanism is based on a conventional micrometer screw with a displacement of 10 mm The micrometer scale Is visible and permits reading to 10 um The drive is powered by a flange mounted DC motor with gearbox The micrometer drive slider moves on a precision crossed roller guide The excellent rolling properties of the guide ensure absence of sticking and sliding friction and therefore no stick slip effect so that this design is ideally suitable for precision displacement systems The micrometer drive is mounted at its back through a 7 mm dia mounting bolt parallel to the working axis The drive can therefore be mounted e g on the non remote control micromanipulator MM 3 in place of the microelectrode holder Specifi
46. 244 High Performance Lab Tables rrrrrrerrrerorororrvrrrvrrrrrererererererenennverrrnsrerererevenenn 245 246 SJETTE Go EEE EN 247 250 Neurolog EE 251 272 Cell Biology Section 273 Microinjection Introduction to Mucromnierton 274 EE 275 EEE EE REN 216 277 PHRTOOA meara e 278 279 EEE EE NE 280 PLI Injector Accessories mmmemeererrinrnmerneervervmmnimnmereereervrmnnnereeee 281 PLS 1 Pico Injection Micromanipulation System 282 Air 1 Ultra Low Noise Air Compressor rovrvrnrvrvrrrrrvrvrnverevereverereverrnrrerenrrrersrererererennn 283 BH 2 Neurophore ee 284 288 Nanoject ll Auto Nanoliter Injector eebe 289 Screw Actuated Air Syringes c cccccecccccsscscscscscecececesesesessscsessscscscseeeseteteeseeeesetseseees 290 Screw Actuated Micrometer Driven Hamilton Syringe renervrvvrrveveverrrrrerererrnrnrnsnnn 290 PM 8 amp PM 4Multi Port Pneumatic Injection System 4 8 Channel 291 292 Micropositioning Micromanipulator Selection Guide ooonenrrrrrrrrrrrrvrvrvererenrvrrrrerrrrrrrrerevernvenennn 293 294 Standard Manual Control Mannulators 295 Dovetail Micromanipulators orororrvrrerrrererorerrrrrnvrrvrverrreverereverarnrssvrnersnevevevevevssnsnnn 296 SM 3 New High Resolutin Motorized Micromanipulator 0 0 297 Stardard Motorized Control Manipulators oovovrrrrerrrerrrrrrrvrvrvnrrvereverereverererrsrnnnnnnr 298 Control Units for Motorized Manipulators ooovo
47. 278 e www warneronline com Pico Injector Continued Specifications Input Gas Pressure Injection Pressure Balance Pressure Fill Vacuum Holding Vacuum Clearing Pressure Injection Time Injection Time Accuracy Duration Mode Time Trigger Pressure Units Pressure Monitor Output Pressure Readout Pressure Display Resolution Injection Count Display Power Input Weight Dimensions HxWxD Warranty Order 75 PSI Recommended 105 PSI Maximum W4 64 1735 0 2 to 60 PSI 413 KPa regulated 0 1 to 9 9 PSI 68 9 KPa regulated multi turn control other ranges available on request Internally produced 0 to 12 PSI 0 to 82 KPa regulated Internally produced 0 to 0 1 PSI 0 to 0 75 KPa regulated W4 64 1736 Input gas pressure unregulated 0 01 to 99 99 Seconds 0 01 Crystal Time Base Internally timed or externally gated Front panel foot switch or external TTL pulse BNC PSI KPa switch selectable BNC Rear Panel 10 mV PSI or 1 mV KPa Inject balance clear output port 3 Digits 0 1 PSI or 1 KPa 0 to 9999 Injections External 9VDC 400mA min Power Supply universal input 90 to 264 VAC 5 44 kg 12 Ib 89 x 432 x 250 mm 3 5 x 17 x 9 8 in H x W x D One year parts amp labor W4 64 1737 Model PLI 100A PLI 100A Plus PLI 100ADeluxe Product PLI 100A Basic Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Ho
48. 3 Fax 203 776 1278 e www warneronline com Specifications Neutralization balance Pump Range Current Pump Digital Meter Display Unbalance Current Current Unbalance Current Mode Current Pump Mode Single Cycle Mode Recycle Mode Time Unit Switch Inputs Analog Input Analog Output Sync Output Output Connector Dimensions HxWxD Weight Specifications Outputs Dimensions H x W x D Weight Order W4 65 0601 Product MS 2 Power Supply with Power Cord Max 2500 nA automatically controlled Compliance 105 V linear constant current source manually adjustable 0 to 500 nA by pump control polarity selected OFF switch 3 digits and sign Pump Switch Digital display reads unbalance ground going current in nA In this mode automatic current neutralization is provided Automatic balance feature is switched off display reads amount of current in nanoamperes passed through balance barrel as adjusted by pump control In single cycle mode start switch or external trigger initiates each cycle In recycle mode once start switch or external trigger is actuated repetitive cycles commence automatically Two basic time units can be selected 10 msec or 1 sec In 10 msec position Eject and Pause time switches of IP 2 Modules can be set to cover time range from 10 to 990 msec with 10 msec resolution In 1 sec position time scale is expanded from 1 to 99 sec with 1 sec resolution Cycle s
49. 307 E Series E Series 45 Body Holders E SERIES 45 Body Holders Glass Order Wire Pellet Connector Port OD mm Model W4 64 0908 Pellet 2 mm Pin No 1 0 E45P M10N W4 64 0909 1 2 E45P M12N W4 64 0910 1 5 E45P M15N W4 64 0911 2 0 E45P M20N W4 64 0912 Yes 1 0 E45P M10P W4 64 0913 1 2 E45P M12P 3 W4 64 0914 1 5 E45P M15P W4 64 0915 2 0 E45P M20P o W4 64 0900 2mmJack No 1 0 E45P F10N S W464 0901 1 2 E45P F12N d W4 64 0902 1 5 E45P F15N W4 64 0903 2 0 E45P F20N W4 64 0904 Yes 1 0 E45P F10P W4 64 0905 1 2 E45P F12P o W4 64 0906 1 5 E45P F15P W4 64 0907 2 0 E45P F20P o W4 64 0924 Wire 2 mm Pin No 1 0 E45W M10N CH wa64 0925 12 E45W M12N W4 64 0926 1 5 E45W M15N W4 64 0927 2 0 E45W M20N W4 64 0928 Yes 1 0 E45W M10P W4 64 0929 1 2 E45W M12P W4 64 0930 1 5 E45W M15P W4 64 0931 2 0 E45W M20P W4 64 0916 Wire 2mm Jack No 1 0 E45W F10N W4 64 0917 1 2 E45W F12N W4 64 0918 1 5 E45W FI5N W4 64 0919 2 0 E45W F20N W4 64 0920 Yes 1 0 E45W F10P W4 64 0921 2 E45W F12P W4 64 0922 1 5 E45W F15P W4 64 0923 2 0 E45W F20P 308 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com E Series E Series 45 Body Holders E SERIES 90 Body Holders Gl
50. 5 mm 6 mm deep Weight 360 g 12 7 02 Order Product W4 73 0011 DC Microdrive Type 864 2 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com micromanipulators gt D E 2 an 4 303 Magnetic Bases W4 69 0225 W4 69 0226 V W4 69 0227 ij i V W4 69 0230 e Holds with powerful magnetic force e Perfect for use with micromanipulators W4 69 0231 e Easy on off control lever r W4 69 0234 Magnetic bases offer a convenient way to mount various tools such as micromanipulators in various positions near to your working site The easy to use on off control lever allows for easy placement of the magnetic base in the off position When the control knob is moved to the on position the magnet is engaged and a powerful magnet field holds the magnetic base 4 and tool securely in place Z En A wide variety of models are available with varying rod sizes mounting d holes and magnetic base mounts to meet every need When combined with our line of lightweight breadboards see page 242 it provides a configurable platform for all of your positioning needs Other Order Product configurations of bases are available please call our Technical Sales W4 69 0235 Sub Pole 10 x 165 mm Dx L Department for details W4 69 0233 W4 69 0232 SJoje ndiueWwo s21u W4 69 0236 Sub Pole 12 x 165 mm D x L W4 69 1066 10 mm Rod Clamp W4 69 1067 12
51. 50 Only Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 333 sJajjnd apadid O Tt gt lt 334 MF 5 and MG 5 Microforge Grinding Center Glass micropipette polishing shaping tipping Bending beveling and grinding all in one compact platform Microforge and Microgrinder e Glass micropipette tip polishing shaping tipping bending bevelling and grinding all in one compact platform e Quick changeable platinum heater and microgrinder as center tools e Precise convenient movement controls for heater grinder pipette locations and optical focus e Universal pipette holder for one to seven barrel pipette holding and 0 180 degree bevelling e Up to 40x long working distance objective and 20x eyepiece optics combination plus scale eyepiece for precision measurement e Adjustable precision power supply for variable grinder speed and heating level e Pressurized air for pipette tip clearing expanding and cooling Foot switch controllable The MFG 5 Microforge Grinding Center is designed for precise and efficient modification of micropipette and multi pipette tips This unique apparatus combines a precision microforge with a full function microgrinder in a compact platform The optical system of the MFG 5 is based on a high power horizontally mounted binocular microscope A precision focusing system with a dual ax
52. MP 107 micropipette multipipette puller to assemble a complete micropipette production work station Specifications Two axial manipulator and coarse fine focusing adjustment Pipette Movement Heater Movement Three dimensional adjustment Pipette Holder Body Horizontal Binocular microscope 180 degree turnable for single or multipipette holding Eyepiece 10x 20xWF Optional 10x Scaled Objective Lenses 4x 10x 0 25 40x Optional 25x 40x 0 6 Long Working Distance Heater Platinum wire Light Two super bright white LED s Air In Output Optional air jet foot switch in output tubing and connector Microgrinder Optional changeable microgrinder One foot switch Accessory Extra foot switch Power Supply 20W 120 240VAC Order Model Product W4 64 1610 MF 5 Microforge W4 64 1611 MG 5 W4 64 1612 MFG 5 W4 64 1613 MFG 5A W4 64 1614 MF 5E W4 64 1615 MG 5E W4 64 1616 MFG 5E Microgrinder Microforge Grinding Center Microforge Grinding Center w Air Microforge 220 240VAC Microgrinder 220 240VAC Microforge Grinding Center 220 240VAC Microforge Grinding Center with Air 220 240VAC W4 64 1617 MFG 5AE Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com NEW Automatic Oscillating Tissue Slicer OTS 5000 Updates and Advantages of the OTS 5000 Series e Cutting window for automatic sectioning allows for faster and more reliable sectioning e Control pad
53. N ESP F12N ESP F15N ESP F20N ESP F10P ESP F12P ESP F15P ESP F20P ESW M10N ESW M12N ESW M15N ESW M20N ESW M10P ESW M12P ESW M15P ESW M20P C gt port jG ST AR fr Q Gi jer _ port Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com E Series E Series Straight Body Holders continued E SERIES Straight Body Holders continued Glass Order Wire Pellet Connector Port OD mm Model W4 64 0999 Wire 2 mm Jack No 1 0 ESW F10N W4 64 1000 1 2 ESW F12N W4 64 1001 15 ESW FI5N Ce W4 64 1002 2 0 ESW F20N W4 64 1003 Yes 1 0 ESW F10P port W4 64 1004 1 2 ESW F12P 7 6 W4 64 1005 15 ESW F15P lear W4 64 1006 2 0 ESW F20P W4 64 1007 Vent 1 0 ESW F10V W4 64 1008 1 2 ESW F12V Fs W4 64 1009 15 ESW F15V lear W4 64 1703 1 7 ESW F17V W4 64 1010 2 0 ESW F20V Vented models are standard with the Warner OC 725 Oocyte Clamp How to Decode E Series Model Numbers Body Style Connector Port Vent S straight F 2 mm jack N no port or vent 45 45 degree M 2 mm pin P with port R right angle 90 V with vent microelectrode holders el LICE e Coupling Glass Size H with handle P Ag Ag CI pellet 1 0 mm W Ag Wire 1 2 mm 1 5 mm 1 7 mm 2 0 mm gt D a an T 4 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com
54. PH1A Pipette Holders Stainless Steel Hose Nut Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Pipette Holder Hardware Replacement Kit for use with PLI PH1 and PLI PH1A Pipette Holders Includes 1 each PLI SSB PLI PC PLI HN and PLI SRG Stainless Steel Pipette Holder PLI PH series Adapter for Eppendorf ECET FEMTOTIP Optional Easy Insert Acrylic Pipette Holder W4 64 1626 W4 64 1627 W4 64 1628 W4 64 1629 A016 1 0 A016 1 2 PLI PPH A016 1 2 Acrylic Pipette Holder for 1 0 mm Pipettes Acrylic Pipette Holder for 1 2 mm Pipettes Acrylic Pipette Holder for 1 5 mm Pipettes Acrylic Pipette Holder for 2 mm Pipettes Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com microinjection gt D o an 281 PLS 1 Pico Injection Micromanipulator System The PLS 1 Pico Injection Micromanipulator System combines our popular PLI 100 pico injector a motorized micromanipulator and magnetic base In a single package uondafurosdnu The PLI 100 Pico Injector delivers a wide range of volumes through micropipettes by applying a regulated pressure for a set period of time The system features 5 pressures inject balance clear fill and hold The injector comes complete with input and output hoses foot switch and pipette holder For precise and convenient movement of the electrode our three axes motor
55. Pipettes amp Pullers 331 Programmable Pipette Pullers 332 333 Microforge Grinding Center MF 5 amp MG 5 334 Tissue Sampling EMS Automatic Oscillating Tissue Slicer OTS 5000 3359 336 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 273 Introduction to Microinjection The use of Glass Needle Fine Glass Microcapillary Pipette based techniques for intracellular extracellular microinjection and perfusion has become a popular procedure in numerous areas of experimental biology research e g In vitro fertilization transgenics etc These techniques can best be described as micro surgical procedures that are conducted on a single cell using either a single Warner and Harvard Apparatus Clark Capillary Glass pages 324 330 or multiple barrel glass micropipette a precision positioning device micromanipulator and a microinjector or microperfusor The micropipettes used in these procedures are formed using a Pipette Puller The capillary glass tubing is heated to its softening point and pulled to create the proper size tip diameter and taper for the desired application The small tip diameters as low as 0 2 um of these micropipettes combined with the high precision of the Micromanipulator and Microinjection Perfusion Apparatus allows for precise and accurate delivery This precision allows for accurate and repeatable injections down
56. Stardard Motorized Control Manipulators 298 Control Units for Motorized Manipulators 299 Joystick Manipulator 300 Ultraprecise Micromanipulators 301 Microdrive Controller Type 864 302 DC Microdrive Controller Type 864 1 303 DC Microdrive Controller Type 864 2 303 Magnetic Bases i 304 Microelectrode Holders E Series Electrode Holders 305 310 Q Series Electrode Holders 311 315 ME Series Electrode Holders 316 317 PE Series Electrode Holders 318 PE30W Series Holders 15 NEW Models for Patch Perfusion 319 MP Series Electrode Holders 320 MHH 25 amp MHH 38 Holders 321 Theta Glass Electrode Holders 321 THP Pressurized Holder for Theta Glass 322 Electrode Holders Replacement Parts 323 Capillary Glass t t tS l l Warner Premium Capillary Glass 324 325 Patch Clamp Capillary Glass E PLI 10 see page 275 PLI 100 see page 276 Mechanical Manipulators see page 295 Motorized Manipulators see page 297 E Series Electrode Holders see page 305 ask Premium Standard Wall Borosilicate see page 324 Programmable Pipette Pullers see page 331 Clark Capillary Glass 327 329 Pipette Sterilization amp Storage Container 330 7 Barrel lontophoresis Micropipette 330 Pipette Pullers i Introduction to
57. amming functions include sequences steps time timing heater level heater control and tip length and actions These units are supplied with input tubing and connector spare heater filament tweezers power cord and manual Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Pipette Pullers Programmable Pipette Pullers continued W4 69 0150 Specifications Pulling Force Pneumatic Multi Barrel Pipette 4 or 7 barrel W4 69 0150 only Each Barrel Pipette 1mm OD Heater Nichrome coil Heater Control Heating Number of Sequences gt Steps in Each Sequence 18 Taper Length Setting 0 5 to 20 mm Pressure 1 Regulator 0 1 to 10 p s i T Pressure 2 Regulator 0 1 to 60 p s i S Pressure Gas Input 30 to 60 p s i Microcontroller 74 general heat levels 24 to 99 54 auto heat levels 45 to 98 25 Actions Pull 1 pull 2 pull 2 cool rotation W4 69 0150 only cool air return Display 20 x4 LCD Power Input 110 240 VAC Power Consumption Max 150 W Dimension Hx W x D 17 8 x 27 9 x 35 6 cm Weight W4 69 0150 6 8 kg 18 Ibs W4 69 0151 5 9 kg 13 Ibs Order Product W4 69 0150 PMP 107 Programmable Multi Barrel Pipette Puller W4 69 0151 PMP 102 Programmable Single Barrel e Beat E go E ER Micropipette Puller W4 69 0161 Unpulled 7 Barrel Pipette for W4 69 0150 Only W4 69 0162 Unpulled A Barrel Pipette for W4 69 01
58. art from its compact size similar to a remote control for a TV set a special feature is the combination of continuous and stepping linear operation In the continuous mode the micrometer drive is operated by the joystick shift in the direction IN or OUT The drive speed is proportional to the displacement of the joystick In the switch mode the micrometer drive is moved by the joystick either IN or Out by a preset step The size of the step can be adjusted continuously with the STEP BS4DTH potentiometer from 0 5 um to approx 0 1 mm per step This operating mode is particularly useful when advancing glass microelectrodes into the cell membrane for intracellular potential recording In order to reduce interference emission to a minimum e g in the input circuit of a microelectrode amplifier special attention has been paid in the design of the instrument to good electrical de coupling and screening The system consists of the Microdrive Controller 864 mains adapter ground cable foil screened connecting cable for micrometer drive and operating instructions Specifications Operating Mode Pulse width modulation under microprocessor control Accuracy Real information on resolution obtainable positioning accuracy compensation of play and min max displacement speed cannot be provided for controller alone these are affected largely by drive mechanics loading sliding and sticking friction in guides operating position and manufacturing toleranc
59. ass Order Wire Pellet Connector Port OD mm Model W4 64 0940 Pellet 2 mm Pin No 1 0 ERP M10N i l W4 64 0941 1 2 ERP M12N W4 64 0942 15 ERP M15N i W4 64 0943 2 0 ERP M20N W4 64 0944 Yes 1 0 ERP M10P QUEE port W4 64 0945 E ERP M12P S W4 64 0946 1 5 ERP M15P y 5 W4 64 0947 2 0 ERP M20P E W4 64 0932 2mm Jack No 1 0 ERP FION E W4 64 0933 1 2 ERP F12N EU W4 64 0934 1 5 ERP F15N 2 W4 64 0935 2 0 ERP F20N z W4 64 0936 Yes 1 0 ERP F10P W4 64 0937 1 2 ERP F12P Du IE port W4 64 0938 15 ERP F15P W4 64 0939 2 0 ERP F20P W4 64 0956 Wire 2 mm Pin No 1 0 ERW M10N ME W4 64 0957 1 2 ERW M12N amp W4 64 0958 15 ERW M15N i W4 64 0959 2 0 ERW M20N W4 64 0960 Yes 1 0 ERW M10P W4 64 0961 1 2 ERW M12P qo W4 64 0962 15 ERW M15P W4 64 0963 2 0 ERW M20P W4 64 0948 2mm Jack No 1 0 ERW F10N W4 64 0949 1 2 ERW F12N p I W4 64 0950 15 ERW F15N G W4 64 0951 2 0 ERW F20N W4 64 0952 Yes 1 0 ERW F10P W4 64 0953 12 ERW F12P CO F W4 64 0954 1 5 ERW F15P P port W4 64 0955 2 0 ERW F20P Vented models are standard with the Warner OC 725 Oocyte Clamp Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 309 E Series E Series Holders with Handle Holders include a 6 3 mm diameter 6 3 cm long handle for mounting in a micropositioner Handles are screwed together except for vented models which are joined by a pin and jack for easy removal from the handle E SERIES wi
60. bium Lead Patch Glass W430 0092 15 116 100 25 OD ID Length i W4 30 0093 15 116 mom Order Model mm mm mm ty W4 30 0097 1 65 1 28 75 190 W4 64 0815 G86150T 4 1 50 1 10 100 225 Wa 30 0095 1 65 1 28 100 190 W4 64 0816 G86165T 4 1 65 1 20 100 190 W4 30 0096 1 65 1 28 150 190 326 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Clark Capillary Glass An old favorite Borsilicate Capillaries The properties of borosilicate glass make it the most popular material among researchers for the fabrication of electrodes and micro pipettes Its low softening temperature combined with its mechanical strength chemical durability high electrical resistivity and Its ability to withstand thermal stress make these capillaries the most widely used in the world Clark borosilicate capillaries are offered in a variety of diameters and wall thickness standard or thin walled with or without an inner filament They are available in fused multi barrel configurations as well as in theta style Single barrel glass is available in 75 mm 3 in 100 mm 4 in and 150 mm 6 in lengths e High quality borosilicate glass e Five outside diameters available e Available with either thin or standard wall e Ratio of outside to inside diameters preserved to tip e Economical Borosilicate Thin Wall without Filament Borosilicate Thin Wall without Filament OD ID Length Order mm m
61. cations Spindle Precision micrometer Displacement Range 10mm Resolution 2 um Drive DC motor 12 V 100 mA nom current Gearbox Spur gearing compensation of gearing backlash is through backlash correction setting of HSE Microdrive Controller Type 864 Foil screened cable with moulded Mini DIN plug suitable for HSE Microdrive Controller Type 864 approx 1 60 m Connection Cable long Scale According to displacement 0 to 10 mm reading accuracy 10 um Case Rigid aluminium black anodized Mounting On back offset 7 mm clamping bolt parallel to working axis Dimensions H x W x D 112 x 25 x 32 cm 4 4 x 1 x 1 3 in sizes without fitted microelectrode holder and mounting bolt Weight 320 g 11 3 02 Order Product W4 73 0003 DC Microdrive Type 864 1 HSE HA Microdrive 864 2 Perfect for intracellular recording The motorized micrometer drive Type 864 2 offers excellent displacement characteristics as required e g for inserting glass microelectrodes during intracellular measurement Together with the control unit HSE HA Microdrive Controller Type 864 the Microdrive Type 864 2 represents a complete alternative to previous hydraulic micro controls for the remote operation and vibration free insertion of the microelectrode into the cell membrane In addition to the compact construction a special feature is the backlash free circulating ball spindle and the limit switch built into the mechanism The circulating ball spind
62. ce allows the tool holder to tilt to one side permitting rapid exchange and easy cleaning of micro tools Stepper motors with 40 000 steps per revolution and single steps of 25 nm enable vibration free operation with no backlash Full travel range Is 25 mm in all three axes eliminating the need for a course manual adjustment The included controller features a 4 line display and includes a front panel switch enabling stand alone operation via joystick The controller comes complete with user friendly WinCommander software a joystick an RS 232 interface cable and motor cables The software can store up to 999 position coordinates for automated operation For users wishing to write their own software a complete set of function commands and a DLL library are included Specifications Resolution Travel range Control method Maximum speed Power Input Weight Micromanipulator Controller 25 nm step 40 000 steps revolution 25 mm each axis Joystick RS 232 software 4 mm sec 120 to 260 VAC switch selectable 1 400 Kg 3 1Kg Dimensions Micromanipulator HxWxD Controller H x Wx D Order W4 64 1731 W4 64 1732 W4 64 1733 Model SM 3 MCL3 SM 3M0 106 x 135 x 135 mm 4 1 x 5 3 x 5 3 in 89 9 x 25 4 x 228 5 mm 3 5 x 1x9 in Description Compact high resolution three axis motorized micromanipulator with MCL3 controller Controller for SM 3 microma
63. ducible performance e Easy to use e Popular applications Injection of mouse frog zebrafish and other oocytes Extracellular brain injections Injection of DNA mRNA microbeads neurotransmitters kinases and other proteins e Most Published Injector The PLI 100 Pico Injector reliably delivers a wide range of volumes through micropipettes by applying a regulated pressure for a digitally set period of time Compressed gas allows the user to deliver desired volumes from femtoliters to microliters while simultaneously holding a cell Whether you need to do large injections into capillaries or very small injections into mammalian nuclei the PLI 100 is well suited for your experiment The PLI 100 has become a favorite of prestigious national microinjection workshops like Cold Spring Harbor Laboratories and other researchers worldwide Other companies have tried to design similar systems but the PLI 100 remains unparalleled in terms of ease of use durability precision and cost Researchers Say The PLI 100 is very robust in constant use in our lab Most importantly the PLI 100 valves prevent cytosol and yolk backflow into my pipette after oocyte injection Its very easy to control The PLI 100 is heavily used on a daily basis and works great I like the fact that I can use the PLI 100 with TTL pulses It works great in conjunction with my imaging system The balance pressure IS
64. e Holders Brass Screw Fitting Type Old Output Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 90 100 Gas Output Quick Connect Disconnect Type New Holding Hose with Cell Release Bulb 9 ft Lx 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Brass Screw Fitting Type Old Holding Hose with Cell Release Bulb 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Quick Connect Disconnect Type New Order Model Product Pipette Holder Adapter Parts W4 65 0013 W4 65 0014 W4 65 0017 W4 65 0015 W4 65 0018 W4 65 0019 W4 65 0020 W4 65 0021 W4 65 0022 PLI PH1 PLI PH1A PLI SRG PLI SRG1 5 PLI SSB PLI PC PLI HN PLI PH KIT PLI PHA Stainless Steel Pipette Holder 130 mm L for 1 to 1 5 mm OD Glass Pipettes Stainless Steel Pipette Holder 80 mm L for 1 to 1 5 mm OD Glass Pipettes Silicone Rubber Gasket Replacement for use with PLI PH1 and PLI PH1A 10 2 cm 12 in Silicone Rubber Gasket Replacement for use with 1 5 mm Glass Pipettes Stainless Steel Bushing Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Stainless Steel Pipette Cap Replacement for use with PLI PH1 and PLI
65. e Luer female Luer and 4 28 female threaded p Microinjection Glass Sizes 1 0 1 2 1 5 and 2 0 mm Handle 6 3 mm diameter x 6 3 cm long threaded to attach to holder D T MP SERIES Holders a Glass Order Body Style Port OD mm Model W4 64 1261 Straight xe barb 1 0 MP S10A G W4 64 1262 1 2 MP S12A W4 64 1263 1 5 MP S15A W4 64 1264 2 0 MP S20A o W4 64 1265 barb 1 0 MP S10B W4 64 1266 12 MP S12B W4 64 1267 1 5 MP S15B W4 64 1268 2 0 MP S20B W4 64 1273 male Luer 1 0 MP S10ML W4 64 1274 1 2 MP S12ML W4 64 1275 15 MP S15ML W4 64 1276 2 0 MP S20ML W4 64 1269 female Luer 10 MP S10FL W4 64 1270 1 2 MP S12FL W4 64 1271 1 5 MP S15FL W4 64 1272 2 0 MP S20FL W4 64 1278 threaded 1 0 MP S10T W4 64 1279 n 28 1 2 MP S12T W4 64 1280 1 5 MP S15T W4 64 1281 2 0 MP S20T Shaft Diameter Threads Inch 320 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com MHH 25 MHH 38 and Theta Glass Holders Microelectrode holders for every application and manufacturer A y MHH 38 and MHH 25 A Diameter Model Diameter MHH 25 6 3 mm Uc MHH 38 9 5 mm Electrode Manifold Holders MHH 25 MHH 38 Device Compatibility Specifications WW Order Model Description W4 64 0218 MHH 25 E and Q Series Electrode Holders The MHH 25 and MHH 38 Holders permit convenient mounting of manifolds and electrode holders or other devices
66. e control and an extremely low dead air volume The capacity of the syringe is 10 ml When required the syringes can be used to generate high pressure or suction The Screw Actuated Syringe is a small compact unit incorporated into a heavy circular base Its convenient dimensions enable it to be ergonomically positioned next to the control lever of a micromanipulator to facilitate single handed operation of both instruments The syringe is connected by hard polyethylene tubing to a micropipette which in turn may be fitted to a micromanipulator or positioning device Suction injection is obtained by turning the metal colored actuator screw control on the top of the syringe The syringe provides a solution to the problem of capillary action when working with small micropipettes A small balance equilibrating pressure can be maintained to offset the effects of capillary action A conveniently situated release button on top of the screw control is provided to achieve equilibration Order Product W4 69 0105 Screw Actuated Air Syringe Screw Actuated Micrometer Driven Hamilton Syringe e Ideal for oil filled injection aspiration e Easy to use e Hamilton syringe incorporated Accurate e Sensitive control e Low dead air volume e High suction pressure e Easy to exchange or replace the syringe This screw actuated syringe is a micrometer actuated syringe mechanism mounted on a small base This syringe Is for oil air assisted micro
67. ed Platforms with Magnetic Clamps orovrrrrrrrrrrrrrrrrrrverrrernnn 60 61 Platform Accessories Interface Cables Themmistors 62 Magnetic Clamp Kit for Series 20 Chambers MK 63 Series 20 amp 30 Stage Adapters nrnrorvnvnvnvvnvnvorvnvnvnvvsvnvnrenenvnvnvenensenesenenvesennenenene 69 Chamber Accessories Coverslips ER 70 Slice Anchors for Series 20 and 40 Chambers ss1ssississiiesisiresisienirerreirsieniennrenn 71 Slice anchors EE EE EE peetsastadedtouadeiner 72 Select 72 Nyon AVES SNE EN EN ia iE i 72 Replacement Suction and Perfusion Tubes ervrnrvrrrrrrrvrrrevereererevenrrrrrrrrrrrrrevevenenn 73 Agar Bridge Reference Electrode Kit oo cccccsecscscscseseeseseseceececsescseseseseseseceeceees 74 Mini Magnetic Clamps cccccccccscscsssescecscssesesesesesssessscscsssesvasacaesesssssssesesessesessescacaes 74 PE Polyethylene Tubing oo ccc ccecccccscssesessecesesesescssesesssesesssetscseeseecesstseesseaceceeeseeeeees 15 Silicone Grease ORRE 75 Suction Tube Upgrade Kit for Series 21 75 Model MDA 1 Petri Dish Adapter with Lamp 76 Model MSC 1 Magnet Spring Clamp Get 76 Perfusion Microfluidics Section Syringe Pumps TEN 78 79 PHD ULTRA Advanced Syringe Bump 80 83 PHYSIO 22 Low EF Syringe Pump for Physiolgical Experiment 84 Peristalti FUNPS eer 85 87 Valve Control Systems Pressurized Perfusion Kits VPPG and VPP geegent eet 88 VC 8M VC 8MLT VC 8 amp VC 8T Valve Control Systems sorrrvrv
68. eded e Light weight acrylic electrode holder e Easy coupling to pressure source e Rated to 100 PSI The new THP holder from Warner Instruments allows quick and easy coupling of theta tubing to a pressure source Complete kits are available for both 1 5 mm and 2 0 mm theta tubing A drop of included hot glue adhesive bonds the theta to a flexible fill capillary tube A barb fitting at the output permits coupling to the pressure source with 1 16 1 D Tygon tubing The light weight fully adjustable holder makes assembly a breeze Included with the THP 15 1 THP acrylic theta tubing holder with integrated handle 1 Package of 10 Flexible Fill Capillary Tubes 350u O D 0 D Package of 10 Feet of 1 16 Tygon tubing Hot Glue Gun Hot Glue Sticks Package of male luer to 1 16 barb fittings 25 pcs pkg Package of 1 5 mm 0 D Theta glass 100 pcs pkg Order W4 64 1620 W4 64 1621 W4 64 1624 W4 64 1642 W4 64 1643 W4 64 0810 W4 64 0811 W4 64 1622 Complete Kit Se Male luer to 1 16 barb Model THP 15 THP 20 THP FTC 35 FTC 45 TG150 4 TG200 4 Handle 6 3 mm diameter Product Pressurized Holder Kit for 1 5 mm Theta Tubing Pressurized Holder Kit for 2 0 mm Theta Tubing Pressurized Holder Only Flexible Fill Capillary Tubes 350 um Flexible Fill Capillary Tubes 450 um Theta Glass 1 5 mm OD Theta Glass 2 0 mm OD Barb Fittting 1 16 Male Luer 25 THP acrylic theta tubing holder with integra
69. el display for pressures and programming information The PM 4 and PM 8 programmable 4 and 8 Channel Pressure Injector systems are designed for one to eight channel intracellular injection and extracellular perfusion These microinjection and perfusion engines are especially designed for pharmacological drug testing molecular biological DNA RNA transferring intracytoplasmic sperm injection and cell electrophysiological applications Combining an advanced micro controller with precision pneumatic components these systems can simultaneously control up to eight injection micropipettes or eight perfusion vials one cell holding pipette and one drain pipette The four PM 4 or eight PM 8 injection perfusion output ports can be controlled separately or combined together to perform actions such as injection capillary action balancing suction or clear up The cell holding output port can produce an adjusted gentle suction to hold a cell eject a pressure to push a cell or completely clear the holding pipette Another drain output port can simultaneously produce an adjusted suction to drain and clear out liquid before the next channel micro perfusion The PM 4 8 can deliver different quantity agents and drugs from picoliters to continuous perfusion All output pressures and vacuums can be regulated and can be displayed real time on the front panel display Previous pressure readings can also be recovered on the display for setting comparison Be
70. ems that have been opened 4 Product s that have been exposed to harmful toxic or hazardous substances Repairs If your product Is out of warranty but requires repair you must contact Harvard Apparatus and obtain a Return Authorization Number and instructions to facilitate the return process All repairs are subject to the following 1 Repair orders charged on a time and materials basis 2 150 per hour for labor with a 150 minimum labor charge 3 All repairs are performed on a first in first out basis only after receipt of a valid purchase order 4 Estimates available upon request 5 Some older products may not be repairable due to component obsolescence Minimum Orders We appreciate all orders and therefore have no minimum order requirement however a small handling fee of 10 will be added to orders below 75 Copyright Harvard Apparatus No part of this catalog may be reproduced in any form by any means electronic mechanical photocopying or otherwise without prior written permission of Harvard Apparatus Harvard Apparatus reserves the right to discontinue any product in this catalog at any time without prior notice Not responsible for typographical errors relative to sizes descriptions and or pricing Note Products in this catalog are for Research Use Only Not for use on humans unless proper investigational device regulations have been followed All Prices subject to change without notice Prices are q
71. en 172 174 Accessories for Warner Micro Incubation Chambers 000008 175 Superfusion Brain Tissue Slice Chamber System ssscsssssses 176 178 Electrophysiology Equipment Section Patch Clamp Equipment nroreernrnrvvnvvnvnvnvnvnvvnvnrenvsvnvnrenenvenenenenvnvenentenenen 180 183 Bilayer Workstation Complete Bilayer Workstation eer 184 Bilayer Workstation Component st 185 Bilayer Clamp Amplifier D hin 186 187 Faraday Cages BEE 188 Bilayer Chambers and Cuvettes BCH M13 amp BCH M22 n se 189 Perfusion Model BCH P Bilayer Chambers oooonrvrrrrererrrrrvererrvererererensrrrerersrrrrnsnsnnn 190 BPS 2 amp LPF 8 Perfusion System for Bilayer a e 191 LPF 8 Bessel Filter ccccccccscscscsssssscecscecscsesesesesesesesesavscacsesesesesesesesecscecsesesessseseesaes 191 SUNStir 3 SUNStir controller SUN 1 lamp and and SPIN 2 stirplate 192 193 RAC 14 Instrument Rack EE 194 BLM Starter EEN 194 HST 1 MBB Head Stage Holder 194 BLM TC Bilayer ett cxcec ces sivestw cd seces detained aied Aastra doug cde tasidccecbeenseeiaces 195 On Site Setup and Training oo cscs cecscscsesesesesssesesescscsssescacscaesesesesesesesessesetseaes 195 Variable Channel Model M72 196 Fixed Channel Model M72 196 Stereo Zoom Microscope ae wes eceaees e EEE e 197 Power Line Conditioner EEN 197 Aquisition Hardware and Software u cccccccccecscssssesssssescscstseeseessseseseessestseetaeeeaees 198 Oocyte
72. er and input hose adapter The deluxe unit includes all the previously mentioned accessories plus an additional foot switch and one pipette holder Accessories Available accessories for the PLI 100 Injection Systems are located on page 281 Specifications Input Gas Pressure Injection Pressure Balance Pressure Fill Vacuum Holding Vacuum Clearing Pressure Injection Timer Pulse Width Injection Count Display Duration Mode Time Trigger Pressure Units Pressure Monitor Pressure Readout Line Voltage Power Usage Meter Accuracy Foot Switches Weight Dimensions H x W x D Accessories Supplied Order W4 65 0001 W4 65 0002 W4 65 0003 Model PLI 100 PLI 100 Plus 70 to 105 p s i 480 to 720 kPa 0 2 to 60 p s i 413 kPa regulated multi turn control 0 1 to 3 5 p s i 68 9 kPa regulated multi turn control other ranges available upon request Internally produced 12 0 p s i 82 kPa unregulated Internally produced 0 to 3 in H 0 0 to 0 75 kPa or 0 to 0 1 p s i regulated Input gas pressure unregulated 0 01 to 0 99 sec in 10 msec steps 1 to 99 sec in 1 sec steps Digital 0 through 9999 Internally timed or externally gated Front panel foot switch or external TTL pulse BNC p s i kPa switch selectable BNC connector 10 mV p s i Inject balance clear output port 100 110 220 240 VAC 220 W 0 1 full scale Inject fill hold and gated provided in plus and delu
73. es 10 to 15 V DC depending on mains supply unit used Nominally 100 mA Output current is monitored continuously to protect drive mechanics and motor switch off limit can be adapted by trimmer potentiometer to suit individual micrometer drive used Motor Voltage Motor Current Overload Protection Continuous Operating Manual displacement proportional to movement of Mode joystick in IN or OUT direction Stepping Operating Mode Step wise displacement by amount set on STEP BS4DTH potentiometer Fast Mode Changes displacement speed for fast movement of micrometer drive Backlash Compensation Backlash in gearing is compensated by means of additional displacement pulse on change of direction pulse length can be adjusted to individual mechanism Monitor LEDs Brightness modulated indicating LED for IN and OUT directions to indicate control voltage produced Beep Short audible signal when triggering pulse in stepping mode or error message on over current switch off Supply 230 V AC 115 V AC through external mains adapter to suit local supply or directly by 12 15 V DC or AC 150 mA Dimensions Wx HxL 45 x 130 x 70 mm 1 8 x 5 1 x 2 8 in Weight 250 g 8 8 02 Note The motorized micrometer drive required for operation is not included with the controller and has to be ordered additionally e g HSE Type 864 1 or Type 864 2 Order Product W4 73 0000 Microdrive Controller 864 115 VAC 60 Hz W4 73 0001 Microdrive Controller 864 2
74. ette 1 W4 69 0154 Input Tubing with Connector p Cord 1 1 for use with dd W4 69 0152 see above d User s Manual 1 1 W4 69 0153 see above Ka W4 69 0150 see pages 332 333 e W4 69 0151 see pages 332 333 Specifications lt 292 Output Channels Input Gas Pressure Clearing Pressure Clear Hold Pressure Injection Pressure Balance Pressure Fill Vacuum Hold Vacuum Drain Vacuum Repeatability Display Accuracy Timer Setting Range Counter Setting Range Savable Sequences Programmable Steps Remote Control Ports Signal Output Ports Power Consumption Four PM 4 or eight PM 8 injection perfusion outputs one synchronized drain out and one cell hold channel 30 to 100 p s i Same as input pressure 0 to 3 p s i regulated 0 05 to 60 p s i regulated 0 05 to 10 p s i regulated 0 to 24 in Hg regulated 0 to 30 in of water regulated 0 to 30 in of water regulated 0 02 p s i 0 05 p s i for pressure 0 2 in for vacuum lt 2 in H 0 10 msec to 327 67 sec 10 msec resolution for whole range 1 to 255 16 sequences 32 steps for each sequence DB9 connector for remote key pad foot switch jack and BNC for negative TTL trigger jack connector for injection foot switch Eight BNCs for injection signal on output port 1 to 4 100 120 or 220 VAC 35 W W4 69 0164 and W4 69 0163 Pulled Pipettes for use with W4 69 0152 see above W4 69 0153 see above W4 69 0155 Output Tubing wit
75. fine adjustment slide with built in miniature DC motors rotating micrometer spindles This motorized fine adjustment has 10 mm 2 1 2 in of travel on each axis and the scales read to 0 01 mm Note For operation these motorized micromanipulators require one of the control units listed on the facing page Detail of Fine Adjustment Control These micromanipulators provide hands off control for fine adjustments Each axis has a controller motor with a flexible coupling to the micrometer spindle which ensures that the probe can be moved with zero vibration They are available with one or two tool electrode holders The micromanipulator with two tool electrode holders has a swing in swing out platform This feature permits the entire x axis platform with tool holder to be swung out to the side for rapid and easy exchange and cleaning of tools capillaries electrodes etc After exchange cleaning of tools the platform swings back to the prior working position without losing time for readjustment The micromanipulators with this X axis tilt should always be mounted in the vertical position to make best use of the tilting fine control Standard Motorized Control Micromanipulator Two One with X Axis Electrode Platform Holder Swing Out Right Handed Clamp Base W4 60 0571 W4 60 0600 Left Handed Clamp Base W4 60 0572 W4 60 0601 20 x 12 x 11 cm Tilt Base W4 60 0574 Note These Standard Motorized Control Micromanipulators requ
76. for exact shipping charges or include your UPS or FedEx account number All checks should be made payable to Harvard Apparatus in U S Dollars and drawn on a U S bank Delivery Options Shipping Shipping charges are added to your invoice Orders ship UPS Ground service unless otherwise requested FOB Hamden CT 06514 Terms and Conditions Return Policy If unsatisfied with a product purchased from Harvard Apparatus you may return the item for credit or replacement You must contact us within thirty days of receipt of your shipment to obtain a Return Authorization Number and instructions to facilitate the return process All returned products are subject to inspection and approval by Harvard Apparatus prior to issuing credit or replacement Products must be in original manufacturer s packaging and include all instructions manuals and inserts Products returned in new condition will be charged a 15 restocking fee or a minimum of 30 00 Products not in saleable condition will be returned to the customer or assessed a refurbishment fee Harvard Apparatus will provide full credit for the following 1 Items not supplied in accordance with your order 2 Items that are defective at the time of receipt Returns not acceptable for credit include 1 Items that have been customized 2 Items that are outdated shelf worn damaged or used and therefore unsuitable for return to stock for resale 3 Chemicals or sterile it
77. g and cleaning Two white LED s are installed in the blade head which provide constant cold illumination of the specimen during the slicing process The OTS 5000 is engineered for maximum stability allowing even the most delicate of tissue to be cut successfully every time The knife holder allows the blade to be well secured and the blade angle set from 10 20 degrees The specimen mount is adjustable on the x y and z axis Blade oscillation is adjustable from 50 5000 cycles minute the knife advance is continuously adjustable with a touch of the keypad from 0 5mm s and the section thickness is adjustable in 1 micron increments ranging from 1 999 microns This precision slicer features automatic touch selection of section thickness blade speed advance and a user defined travel window so that consecutive sections may be cut easily and rapidly Other OTS 5000 features include Touch pad control of the blade height slice counter slice thickness precise bar graph displays of blade oscillation and advance speeds The OTS 5000 is designed for easy operation and maintenance The specimen collecting tray is removable and easy to clean All controls may be set with one hand with options for multiple slicing modes The unit comes complete with gooseneck magnifying lens media tray 2 5 wide and pedestal specimen vice holder blocks as well as a foot switch with D cable A large tray 3 5 wide is available as an option and comes complete wit
78. h Connector and 4 5 ml Vial for use with W4 69 0152 see above W4 69 0153 see above W4 69 0150 see pages 332 333 W4 69 0151 see pages 332 333 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Micromanipulator Selection Guide Manipulator Selection Guide Chart Ratio Adjustment Mechanical Manipulators 1 15 to 1 150 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Coarse Movement Travel X 65 mm Y 29 mm Z 65 mm X 37 mm max Y Z 20 mm max X 37 mm max Y Z 20 mm max X 37 mm Y 20 mm Z 25 mm Resolution X Y Z 100 um X Y Z 100 um X Y Z 100 um Fine Movement Travel Z fine 2 2 mm X 10 mm max X 10 mm max Z fine 3 mm X Y Z 25 mm max Resolution Mounting Z fine 10 um X 10 um X 10 um X Y Z 5 um Features Options Mounting Base 0 5 in Rod Clamp Tiltable Arm 10 12 mm 0 5 in Single Rod Clamp Electrode 10 12 mm 0 5 in Dual Tool Rod Clamp Electrode Tilting Base Holder Mounting Base Joystick 0 5 in Rod Clamp Integrated 25 Tilting Base Large Spindle Knobs High Mass for Stability Brass Construction Order W4 72 6030 W4 72 6031 W4 72 6032 W4 72 6033 W4 64 0056 W4 64 0055 W4 60 0569 W4 60 0570 W4 60 0582 W4 60 0583 W4 60 0594 Page 296
79. h fixed stage and adjustable pedestals Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com tissue sampling gt D 2 an T 335 Sales Subsidiaries amp Authorized Distributors Subsidiaries CANADA Harvard Apparatus Canada Attn Sales Department 6010 Vanden Abeele Saint Laurent Quebec H4S 1R9 Canada phone 514 335 0792 800 361 1905 CAN only fax 514 335 3482 e mail sales harvardapparatus ca website www harvardapparatus ca T FRANCE Harvard Apparatus S A R L Attn Sales Department 6 Avenue des Andes Miniparc Bat 8 91952 Les Ulis Cedex France phone 33 1 64 46 00 85 fax 33 1 64 46 94 38 e mail info harvardapparatus fr website www harvardapparatus fr GERMANY Hugo Sachs Elektronik Harvard Apparatus GmbH Gruenstrasse 1 D 79232 March Hugstetten Germany phone 49 7665 92000 fax 49 7665 920090 e mail info hugo sachs de website www hugo sachs de SPAIN Panlab S L Harvard Apparatus Spain C Energia 112 08940 Cornell Barcelona Spain phone 34 934 750 697 Intl Sales phone 934 190 709 Sales in Spain fax 34 934 750 699 e mail info panlab com website www panlab com ma UNITED KINGDOM ZAIN Harvard Apparatus Ltd Attn Sales Department Fircroft Way Edenbridge Kent TN8 6HE United Kingdom phone 44 1732 864001 fax 44 1732 863356 e mail sales harvardapparatus co uk website
80. ial pipette holder can conveniently and precisely position single or multi barrel pipettes The pipette holder is capable of rotating through 180 to allow a multitude of beveling angles and pipette bending positions The MFG 5 uses high quality optical components which include 10x and 20x wide field eyepieces and 4x 10x 0 25 and 40x 0 6 long working distance plan achromatic objectives The 10x wide field scale eyepiece can be used as an independent and accurate measurement tool Two high intensity LED s provide a powerful light source Microforge An included foot switch or a heat speed adjustment dial can be used to regulate the attached heater or grinder In addition to the included heater grinder foot switch the MFG 5A has an second foot switch for the regulation of pressurized air Air from an external source is connected to the Microforge Grinding Center and when the foot switch is activated is directed to an output channel Pressure air from this source can be used for clearing a pipette of dust after grinding or for expanding or cooling a pipette during forging The MFG 5 can be purchased comprised of the microforge only the microgrinder only or comprised of both components For the latter system the microgrinder and the microforge mini heater can be quickly and easily exchanged since both components use the same quick connector and temperature speed control system Finally the MFG 5 can be combined with a PMP 102 or a P
81. iminates dilution caused by capillary action and aids in the prevention of clogging Wasteful continuous injection which often occurs when no separate balance pressure is offered by an injection device is avoided Fill Hold There are two built in vacuum generators to fill a micropipette from the tip and to hold suspended cells Filling the pipette from the tip is easier than back filling The PLI 100A features a Fill Vacuum adjustment on the front panel Suspended cells can be held with a second holding pipette The holding vacuum s range accommodates most cell types Clear A high pressure pulse can be used for clearing a pipette should it happen to clog This is particularly useful when working with pipettes sized for smaller volumes Electrical Connectors BNC type connectors are available at the front panel to ease integrating the Pico Injector with other equipment Synchronization of injections to other stimulations or recordings is therefore possible Selection Guideline The PLI 100A is available in three packages Basic Plus and Deluxe The Basic unit is supplied with an input hose output hose holding hose and input hose adapter The Plus unit also includes a foot switch pipette holder and input hose adapter The Deluxe unit includes all the accessories included with the Plus and an additional foot switch and pipette holder Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1
82. ing 7740 glass is offered in a choice of diameters lt in standard or thin walled format and either with or without inner OD ID Length filament they are additionally available in Theta style Single barrel Order Model mm mm mm Oty Pkg glass is available in 3 lengths 75 100 and150 mm Theta glass is W4 64 0786 G100F 3 1 0 0 58 75 500 offered in 100 mm lengths only W4 64 0787 G100F 4 1 0 0 58 100 500 W4 64 0788 G100F 6 1 0 0 58 150 500 S sen gs W4 64 0789 G120F 3 1 2 0 69 75 350 pecifications ee SCH W4 64 0790 G120F 4 12 069 100 350 SS 13 B C W464 0791 G120F 6 12 0 69 150 350 7 nee W4 64 0792 G150F 3 15 0 86 75 225 23 W4 64 0793 G150F 4 1 5 0 86 100 225 Softening Temp 821 C i W4 64 0794 G150F 6 1 5 0 86 150 225 Dielectric Constant 4 6 W4 64 0795 G200F 3 2 0 1 16 75 125 W4 64 0796 G200F 4 2 0 1 16 100 125 324 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Premium Capillary Glass continued Premium Thin Wall Borosilicate with Filament OD ID Length Order Model mm mm mm Oty Pkg W4 64 0798 G100TF 3 1 0 0 78 75 500 W4 64 0799 G100TF 4 1 0 0 78 100 500 W4 64 0800 G100TF 6 1 0 0 78 150 500 W4 64 0801 G120TF 3 1 2 0 94 75 350 W4 64 0802 G120TF 4 1 2 0 94 100 350 W4 64 0804 G150TF 3 1 5 1 17 75 225 W4 64 0805 G150TF 4 1 5 1 17 100 225 Premium Theta Glass 100 pcs pkg OD ID Septum Length Order Model mm mm mm mm W4 64 0810 TG150 4 1 5 1 0 0 2 100
83. ire one of the control units described on page 299 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Control Units for Motorized Manipulators Hands off control for fine positioning Choice of two control units with increasing complexity e Push button control unit e 3 direction joystick control unit W4 60 0577 Push Button Control Unit Push Button Control Unit e For either step or continuous motion e Simple push button control Movement forward or back in each of three axes is controlled by six push buttons The amount of time the button is depressed determines the type of movement that occurs By pressing a button for less than 1 second a step movement is provided Pressing the button for more than 1 second activates continuous movement until the button is released This control unit has two control knobs one for step size and one for continuous motion speed The Step size knob ranges from 0 5 to 10 microns graduated in 0 5 micron increments The continuous motion knob provides speeds up to 0 2 mm sec The step size and speed are equal for all three axes Backlash on reversing directions has been essentially eliminated with this control unit At each reversal an additional current pulse compensates almost completely for gear spindle slack This is particularly important in the step mode as it eliminates dead steps when the mo
84. ized micromanipulator and push button controller are included This micromanipulator features hands free spatial resolution of 0 5 um which enables positioning in the sub micro range Flexible motor coupling ensures no vibration of probe during movement o se z e The MB B magnetic base provides a convenient position device for the micromanipulator Order Model Product W4 64 1608 PLS 1 Pico Injector Micromanipulator System W4 65 0002 PLI 100 Plus Pico Injector W4 60 0571 Right handed motorized micromanipulator W4 60 0577 Push button controller W4 64 0060 MB B Magnetic base 282 Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Model Air 1 Ultra Low Noise Air Compressor e Suitable for installation directly at point of use e Lowest noise and vibration level in the market 45dB A you won t even know it s on e Complete compressed air package e Ideal for use with our microinjection systems When a reliable supply of clean and quiet compressed air is required this oil lubricated compressor is the perfect choice The noise level is 45 dB A far below the level of normal conversation Quiet vibration free and reliable this compressors compact design easily allows mounting at the place of use The oil lubricated piston compressor is supplied ready for use The unique synthetic SJ 27 oil designed especially for AIR 1 optimizes the lubrication
85. le requires no maintenance and operates virtually without friction unlike a micrometer spindle with sliding bearing Freedom from backlash is achieved through the use of factory selected balls and is maintained through a very long operating life Absence of sliding friction inside the circulating ball spindle ensures freedom from the so called stick slip effect As a result the mechanism accurately follows even the slightest displacement settings Specifications Spindle Drive Hardened and ground circulating ball spindle freedom from axial play through use of selected balls Resolution 1 um in both directions 50 N 25 mm 50 and 75 mm options also available Maximum Loading Displacement Range Bi Directional Repeatability 2 um Uni Directional Repeatability 0 1 um Drive DC motor with flanged gearbox 12 V 170 mA nom current Gearbox 76 1 with backlash optionally without backlash backlash compensation normally through backlash correction adjustment of HSE Microdrive Controller Type 864 Foil screened cable with moulded Mini DIN plug suitable for HSE Microdrive Controller Type 864 approx 1 60 m L Connection Cable Limit Switch To protect drive unit wired internally with evaluation of displacement direction Scale To suit displacement 0 to 25 mm Case Rigid aluminium black anodized Mounting Clamping fitting 12 mm D and 10 mm clamping width Dimensions Length 196 mm motor case D 20 mm Spindle Connection M6 x 0
86. ller On 2 Axes On 3 Axes With Display W4 60 0586 W4 60 0590 Without Display W4 60 0588 W4 60 0592 Order Product W4 60 0594 Ultraprecise Manual Control Micromanipulator W4 60 0604 Tool Holder pkg of 3 W4 69 1066 10mm Rod Clamp for use with Magnetic Bases W4 69 1067 12mm Rod Clamp for use with Magnetic Bases Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com micromanipulators gt D a ee T 4 301 10 e ndiuewoJoIwW O pt lt 302 HSE HA Microdrive Controller 864 Hands off control for precise positioning e Single axis motorized micrometer drive controller e Alternative to hydraulically operated micro drive The HSE HA Microdrive Controller Type 864 is a single axis control unit with microprocessor control for operating motorized micrometer drives vernier controls with DC motor It has been specially developed to meet the requirements in physiological and pharmacological research It is ideally suitable as control unit for the remote operation and vibration free movement of microelectrodes or capillaries e g in intracellular potential recording patch clamp experiments or stereotaxic investigations in the brain When used with either the micrometer drive HSE HA 864 1 or 864 2 see page 303 the Microdrive Controller thus represents a complete alternative to hydraulic micro drives which have now become very expensive Ap
87. m mm Oty W4 30 0037 1 0 0 78 15 500 W4 30 0035 1 0 0 78 100 500 W4 30 0036 1 0 0 78 150 500 W4 30 0049 1 2 0 94 75 350 W4 30 0047 1 2 0 94 100 350 W4 30 0048 1 2 0 94 150 350 W4 30 0064 1 5 1 17 50 225 W4 30 0065 1 5 1 17 75 225 W4 30 0062 1 5 1 17 100 225 W4 30 0063 1 5 1 17 150 225 Discounts on Quantity Purchases Discount apply to only single part number quantities no mixing 1to 4 none 5 to 9 5 10 10 Specifications Composition 80 9 SiO 12 9 B 0 4 4 Na 0 1 8 ALDO Softening Temp 815 C Dielectric Constant 4 1 Borosilicate Standard Wall without Filament 0 Borosilicate Standard Wall without Filament OD ID Length Order mm mm mm Wall Qty W4 30 0018 1 0 0 58 15 0 21 500 W430 0016 1 0 0 58 100 0 21 500 W430 0017 1 0 0 58 150 0 21 500 W4 30 0043 1 2 0 69 715 0 25 350 W4 30 0042 1 2 0 69 100 0 25 350 W4 30 0041 1 2 0 69 150 0 25 350 W4 30 0056 1 5 0 86 75 0 32 225 W4 30 0054 1 5 0 86 110 0 32 225 W4 30 0053 1 5 0 86 100 0 32 225 W4 30 0055 1 5 0 86 150 0 32 225 W4 30 0073 2 0 1 16 75 0 42 125 W4 30 0070 2 0 1 16 100 0 42 125 W4 30 0071 2 0 1 16 150 0 42 125 W4 30 0127 3 0 1 62 715 0 69 55 W4 30 0080 3 0 1 62 100 0 69 55 W4 30 0081 3 0 1 62 150 0 69 55 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com capillary glass gt 5 S aa T 4 327 Clark Capillary Glass continued Borosilicate with Filament Boro
88. mV full scale in p s i g setting 0 to 700 mV full scale in kg cm2 setting Lemo miniature connector TTL pulse time incident with output pressure pulse Red LED Green LED W4 65 0604 PPM 2 Pneumatic Pump Module with Input Output Hose 7 Barrel lontophoresis Micropipette The MS 7P Micropipette has been designed for iontophoretic use as well as pneumatic ejection of drugs The standard blank consists of seven barrels Pyrex Omegadot tubing 1 5 mm outer diameter and 1 2 mm inner diameter pre pulled to 2 mm formation as shown For complete details see our Warner Instruments Cell Biology Catalog Order Model W4 65 0207 MS 7PB W4 65 0208 MS 7MT Micropipettes Micropipette 7 Barrel 1 5 mm OD Borosilicate Glass Pre Pulled Plank 20 per box Micropipette 7 Barrel 1 5 mm OD Borosilicate Glass Fully Pulled Approx 20 um Tip 10 per box Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Nanoject Il Auto Nanoliter Injector Micromanipulator and Stand sold separately e Lower volume 2 3 nl capability e Positive displacement technology e 2 3 to 69 0 nl range of volumes e Minimal vibration e New improved high torque motor e Membrane key pad e Improved micropipette holder e Two speed injection fill rates e Auto Home capability The Nanoject Il is the latest development in the Nanoject family of microinjectors One unique features of the Na
89. n time scale is expanded to 1 to 99 sec with 1 sec resolution Power 115 VAC 50 60 Hz 100 or 220 VAC optional Dimensions H x W x D 21 x 47 x 35 cm 8 75 x 19 x 14 in Weight 5 5 kg 12 Ib PPS 2 Mini Frame System mainframe power supply and CM 1 Control Panel PPS 2 Mini Frame PPM 2 Modules not included 5 The rack mountable PPS 2 Mini Frame was designed as a less expensive si alternative to the BH 2 for those applications that require pressure ae injections only The PPS 2 is a multichannel pneumatic pumping system Application MNS D designed especially for short term pressure ejection of small quantities of Working Unit fluids through micropipettes The system can operate with up to four PPM Mini Frame Power Supply and Control Panel plus four 4 2 Pump Modules Each module can be programmed to its own schedule of modules selected from PPM 2 and DP 1 ejection and pause times coordinated with the other modules Ejection and pause times cover a range of 10 to 990 milliseconds with 10 millisecond resolution and 1 to 99 seconds with 1 second resolution Order Product z Continuous and cyclical modes of operation are also available W4 65 0606 PPS 2 Mini Frame with Power Supply Control lt System Configuration Module and NL 952 Power Cord and Manual The PPS 2 system Order W4 65 0606 includes a Control Module and a W4 65 0604 PPM 2 Pneumatic Pump Module with power supply It is pre wired to accept up to four PPM
90. nceesesl 342 3032PH Inverted Phase Contrast Microscope 343 GIN ee ProgRes Digital Microscope Cameras mmmmvenrvrvrvevvrvrvrverevererrrrrererrrrrrsrerevennnn 344 348 HE ee EEE AN PEN 349 352 Illuminators Intralux 5100 Fiber optic Cold Halogen Light Sources sssssssissisesresiesireieiesreenen 353 V Lux 1000 Fiber Optic Cold Light zouge 354 NCL 150 Fiber optic Cold Light Source 354 IntraLED 2020 Fiber optic Cold Light Source 355 Light Source Ir 355 Spare Lamps and Power pre ce scaicescest vcascossxocecsesesicishossiicyeasdeddceacdecesseehidenddec 356 Ring Light EE 356 360 Gooseneck Lightguides and Filters sornrrrrrrrrrrrrrrverrrrrereveverrrrrererrrrrrrrerernnnnnn 361 362 Articulating Arm with Heavy Steel Base 363 Modular Accessory stemm 363 Glass Light Fiber Backlights hu msummusseeiemnmesernnrerenisiebnsjeueminden 364 Brightfield Darkfield B stL nnamssmnssnssmnet gen enin tndenvnejeentedendsindde 364 HAT EO EEE ee eee eee er 365 Ne E 368 372 Dissolved Oxgyen Meter and Electrodes cccscsssssseseseseseseeeeees 373 374 E E E TT 375 383 ET NE SE TEA E E E 384 395 Electroporation Section 399 Decision Guide EN 398 Cross Over Guide E 399 UTC 400 401 Generators SO age 206 Gag oes cc pa pa cea eaccea cata eaten ade pan descent naeeteasdec EE 402 PG 403 MP 404 PN 405 ECM 830 High Throughput System acs ees 406 ECMP 630 High Throughput System seonrnrnervrrvrrrrverererere
91. nipulator Includes RS 232 interface cables software and joystick Compact high resolution three axis motorized micromanipulator without controller s10 e ndiuewoJoiwW o se z z e lt 298 Standard Motorized Control Micromanipulators Hands off control for fine positioning W4 60 0571 Standard Motorized Control Micromanipulator with W4 60 0577 Push Button Control Unit Tool Holder Included e Requires one of two control units offered on the facing page e Available with choice of 1 or 2 tool electrode holders Tilt base or clamp mounting Right or left handed use e 20 mm of travel in lateral Y axis and vertical Z axis graduated in 0 1 mm increments e 37 mm of travel in probe direction x axis graduated in 0 1 mm increments e All three control knobs located in single plane for ease of operation and close positioning of several instruments side by side e For use with microscope magnifications up to 250x e Positioning in sub micron range is possible e Smallest step size is 0 5 um e Micromanipulator does not have to be touched for fine adjustment e Flexible motor coupling ensures zero vibration of probe during movement This Standard Motorized Micromanipulator has the same manual coarse adjustment for each of the three axes graduated in 0 1 mm increments X axis 37 mm of travel Y and Z axes 20 mm of travel each On each of the three axes there is installed an additional
92. njection gt D d ee T 291 PM 8 and PM 4 8 and 4 Channel Pressure Injection Systems continued Order Model W4 69 0152 PM 4 W4 69 0153 PM 8 Options and Accessories W4 69 0154 IP 1 W4 69 0155 OP 1 Product 4 Channel Pressure Injection System W4 69 0152 4 Channel Pressure Injection System 8 Channel Pressure Injection System Input Tubing with Connector Output Tubing with Connector and 4 5 ml Vial W4 69 0156 OP 2 Output Tubing with Connector only W4 69 0157 FSW W4 69 0158 PM KP W4 69 0160 RK 2 Footswitch Remote Key Pad Rack Mounting Kit with Handles for PM 4 8 Pulled Seven Barrel Pipette each W4 69 0163 PP 7 Below are the components supplied with each of the Pressure Injection W4 69 0164 PP 4 Pulled Four Barrel Pipette each Systems W4 69 0165 HOLD 1 Holder for Single Barrel Pipette Pressure Injection System PM 4 PM 8 W4 69 0166 HOLD 4 Holder for Four Barrel Pipette Order Product Oty Oty W4 69 0167 HOLD 7 Holder for Seven Barrel Pipette WA 69 0154 IP1 Input Tubing with Connector 1 1 e W4 69 0156 Output Tubing with W4 69 0156 Ge Ge with 6 10 SN omme W4 69 0152 see above W4 69 0157 FSW Foot Switch 1 1 W4 69 0153 see above g W4 69 0150 see pages 332 333 W4 69 0164 PP 4 Pulled 4 Barrel Pipette 1 W4 69 0151 see pages 332 333 e W4 69 0166 HOLD4 Holder for 4 Barrel Pipette 1 W4 69 0163 PP 7 Pulled 7 Barrel Pipette 1 W4 69 0167 HOLD7 Holder for 7 Barrel Pip
93. njector Basic Model and Acces sories Shown Above Plus One Each PLI FS Foot Switch PLI PH1 Pipette Holder and PLI IHA Input Hose Adapter Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com PLI 100 and PLI 90 PLI 100 and PLI 90 Accessories Order Accessories W4 65 0029 W4 65 0030 Hosing W4 65 0006 W4 65 0007 W4 65 0008 W4 65 0009 W4 65 0010 W4 65 0011 W4 65 0012 Model PLI FS PLI RM PLI IHA PLI IHO PLI IHN PLI OHO PLI OHN PLI HHO PLI HHN Product Footswitch for Inject Hold Fill or Gate Order more than one if frequent use of footswitch is needed for more than one of these functions Frame to Mount PLI 90 or PLI 100 in 19 in Wide Instrument Rack Input Hose Adapter in Male WAT Pipe Thread Gas Bottle Regulator Fitting Connects with PLI IHO N Gas Input Hose Tygon Tubing 9 ft x 0 250 in 6 35 mm x 0 125 in 3 18 mm x 0 062 in 1 57 mm Lx OD x ID x Wall Thickness Connects to PLI IHA and PLI 90 100 Gas Input Brass Screw Fitting Type Old Gas Input Hose Tygon Tubing 9 ft L x 0 250 in 6 35 mm OD x 0 125 in 3 18 mm ID x 0 062 in 1 57 mm Wall Thickness Connects to PLI IHA and PLI 90 100 Gas Input Quick Connect Disconnect Type New Output Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipett
94. noject Il is the use of a high torque motor to provide smooth operation resulting in substantially reduced tip movement A new collect configuration holds the micropipette more securely thereby reducing air infiltration and oil leakage The two speed fill inject mode allows the user to fill and inject at half speed facilitating the use of smaller micropipettes The handling of more viscous samples is also improved All of the above factors enhance the precise delivery of sample Volumes for the Nanoject II range from 2 3 n to 69 nl The smaller tips and smaller injection volumes of the Nanoject II allow users to perform injections into other specimens applications that previously could not be performed Positive displacement technology and the use of precision micropipettes eliminate the need for tedious calibration when the viscosity of the sample changes Contaminants in the sample do not hinder or change the injection volume as with some other injectors An optional universal mounting adapter provides easy mounting of the Nanoject II to a variety of micromanipulators and stereotaxic frames Needs Micromanipulator to attach to Support Stand Universal adapter now included for all NJ2 s Specifications Operation Automatic Glass Properties Softening point 780 C Glass Dimensions 1 14 x 0 053 mm 0 045 x 0 021 in OD x ID H Injection Speed Fast 46 nl sec Slow 23 nl sec Fill Speed Fast 46 nl sec Slow 23 nl sec O Empt
95. olution Trigger Mode When the TRIG switch is energized the eject time interval will be started by virtue of the incoming trigger pulse applied to the respective inputs on the BM 2 Balance Module Eject timing interval can be preset covering a range from 10 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second resolution Gate Mode When the GATE switch is energized the eject current will be started by virtue of a gate input signal applied to the respective trigger gate terminals on the BM 2 Balance Module The eject and pause time settings are not operative in this mode since the eject time function is slaved to the duration of the gate input Continuous Mode When the CONT switch is energized the ejection pump current is continuously maintained Termination Mode When the TERM switch is energized the output is automatically diverted from the preparation into an internal dummy load 100 MQ This function is particularly useful for testing of possible instability in the preparation pipette Analog Input The analog input terminal is available to facilitate externally controlled Current pumping action An external voltage applied to the input will generate a pumping current at a ratio of 5 millivolts nanoamperes This current will be summated with any preset pump current governed by both the retention and ejection controls The combined magnitude and sign of the summated pumping current is
96. on because of their large size 1 0 mm Specifications Input Gas Pressure Injection Pressure Injection Time Injection Time Accuracy Pressure Display Injection Count Display Trigger Mode Pressure Monitor Output Power Input Weight Dimensions HxWxD Warranty Order Model W4 64 1707 PLI 10 W4 64 1626 A0161 0 W4 64 1627 A0161 2 W4 64 1628 LPLI PPH 75 PSI Recommended 105 PSI Maximum 0 2 to 60 PSI 413 KPa regulated 0 01 to 99 99 Seconds 0 01 Crystal Time Base 3 2 Digits 0 1 PSI or I KPa Resolution 0 to 9999 Injections Front Panel Footswitch TTL Gate In BNC Rear Panel 10 mV PSI or 1 mV KPa External 9VDC 400mA min Power Supply universal input voltage 90 to 264 VAC 2 3 kg 89 x 215 x 175 mm 3 5 x 8 5 x 6 9 in One year parts amp labor Product Pico liter includes input hose output hose and foot switch Acrylic pipette holder for 1 0 mm pipettes Acrylic pipette holder for 1 2 mm pipettes Acrylic pipette holder for 1 5 mm pipettes W4 64 1629 A0162 0 Acrylic pipette holder for 2 0 mm pipettes uondafurosdnu o e z z e lt 276 PLI 100 Medical Systems Pico Injector A full featured workhourse microinjector W4 65 0001 PLI 100 Pico Injector e 5 pressures inject balance clear fill and hold e Reliable e Femtoliter to microliter injections e Repro
97. onal fine controls allow this second tool electrode holder to be tilted and swiveled Tilt Base or Clamp Mounting Each micromanipulator is offered with a choice of either a tilt base or a clamp to mount the micromanipulator on a 13 mm in OD vertical rod Other clamps are available as accessories For a selection of stands with 13 mm in OD rods see the Stronghold Clamps Stands and Lattices on the Harvard Apparatus website www harvardapparatus com The tilt base permits the micromanipulator to be tilted 80 from the vertical The manipulators with clamp mount are supplied with a 7 In to 10 mm bushing which permits mounting on a magnetic base see page 304 Standard Manual Control Micromanipulator One Holder Two Holders Right Handed Clamp W4 64 0056 W4 60 0569 Tilt Base W4 64 0058 Left Handed Clamp W4 64 0055 W4 60 0570 Tilt Base W4 64 0057 Order Product W4 69 1066 10 mm Rod Clamp for use with Magnetic Bases W4 69 1067 12 mm Rod Clamp for use with Magnetic Bases W4 60 0604 Tool Holder pkg of 3 W4 64 1652 Rod Clamp in W4 64 1652WB Rod Clamp in with A to 10 mm bushing Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com micromanipulators gt D E an T 295 10 e ndiuewoJoiw o se z 2 E 296 Dovetail Slide Micromanipulators For applications requiring a robust holder e Brass dovetail slide const
98. ow noise MS 2 Power Supply W4 65 0601 Features Blank Panel DP 1 Dummy Module W4 65 0605 used to maintain Successfully utilized in key laboratories around the world for over 20 timing if less than 5 Pump Modules are installed years with many journal articles published as a result One Model OC 01 output cable W4 65 0215 Modular design buy what you need now and add additional modules One Model NL 952 lemo cable W4 65 0285 later as your protocol evolves Accommodates 7 barrel micropipettes supports the most complex and demanding injection and recording protocols Extremely low noise 105 volts compliance allows rapid iontophoretic injection with even the smallest micropipette tips Automatic current neutralization for minimal electrical artifacts 284 Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection continued BH 2 Mainframe Chassis The BH 2 Mainframe Chassis is pre wired to accept one Control and Balance module and five lontophoretic Pump Modules and or Pneumatic Pump Modules The selected modules are specified by the researcher and depend on applications Although all components are ordered separately the unit is assembled calibrated and balanced by Harvard Apparatus before shipping BM 2 Balance Module Balance and More In addition to providing current neutralization a
99. pillary tubings softening point temperature is reached a mechanical parallel pulling force is applied to each end of the capillary tube and it is pulled to the desired tip diameter and profile Depending on a number of preset variables such as capillary material type temperature filament type and pulling force numerous types of tip profiles and tip diameters can be fabricated Using this technique tip sizes as small as 0 02 um can be achieved Pipette pullers can range in complexity from a simplistic spring counter weight mechanical puller to highly sophisticated programmable microprocessor controlled pullers To reshape the pipette or to fuse the tip into a different geometry a microforge system is used A microforge uses a heating element microscope illumination micromanipulators and microtools to change the shape of or contact fuse other elements to the micropipette pipette by heating certain sections of the micropipette to either soften for bending or melting fusing the micropipette Some types of microforges can also be fabricate metal and glass microtools Harvard Apparatus manufactures and sells a complete range of high quality equipment and supplies to fabricate micropipettes e Beat a 2 a E ER 2 Cell Biology Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 331 sJajjnd apadid CHA z e 332 Pipette Pullers Programmable
100. plitude adjustable by front panel dial from 0 to 50 nA polarity automatically set opposite to ejection current polarity socket x3 Lemo miniature receptacle ground referenced 5 mV nA input impedance 100 kO socket x3 Lemo miniature receptacle ground referenced 5 mV nA socket x3 Lemo miniature receptacle TTL pulse time incident with eject pulse Digital Meter Display 3 digits and sign Metering System Switch in center nA position digital display reads total current in nA passed through micro electrode pipette sum of retention and ejection current microinjection Switch in either pos or neg MQ position digital display reads actual electrode barrel resistance in MO derived by passing positive or negative constant current 50 nA through electrode pipette Digital display will flash whenever electrode barrel resistance gt D iz an T GA exceeds working range of current pump i e when electrode resistance times current exceeds compliance of 105 V Depressing switch will cause digital display to read voltage across pipette 287 uonsdaluio1dW o L z z e 288 Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection continued PPM 2 Pneumatic Pump Module Designed specifically for pressure injection of drugs in pharmacological studies of drug evoked responses i e synaptic discharges contraction etc Emphasis has been given to pressure control
101. r a digitally set period of time Pressure to the pipette is controlled precisely through a multi turn regulator and is reported digitally for reproducibility Injection time is set using an optically encoded circuit which permits fine and coarse settings from a single knob The system timer can be controlled in three ways front panel push button foot switch and external trigger input BNC Whether you need to do large injections into capillaries or very small injections into mammalian nuclei the PLI 100A is up for the task Pressure Capabilities The PLI 100A features two negative and three positive pressure pneumatic capabilities The negative or vacuum functions allow the user to 1 Fill micropipettes from their tips reducing waste of pipettes 2 Provide a means to secure and manipulate a cell using holding pipette The positive pressures allow the user to e Eject precise amounts of fluids e Create a balance pressure which prevents backflow into the micropipette following an injection e Clear a micropipette of material in it e COLTER IM ESTON e amp Unique Features The PLI 100A s important Balance Hold and Clear functions are features not found on other microinjection units Balance In addition to the ejection pressure the Pico Injector offers a secondary balance pressure This adjustable pressure keeps a positive pressure on the injection pipette before and after injections This el
102. r precision and accuracy and it meets or exceeds the performance of units on the market costing twice as much The OTS 5000 now features a new innovative design of the slicer head which minimizes Z Axis vibration This improvement dramatically improves slice surface integrity and is especially beneficial in the use of fresh tissue where IR DIC imaging methods are used The unique blade holder has the ability to accept sapphire blades as well as razor blades To minimize the time between harvesting consecutive slices the OTS 5000 features a user programmable travel window cutting window This allows the section cutting range of the blade to be customized to the specimen sample In conjunction with the home button the programmable window guarantees rapid sectioning of even the largest of specimens As well the OTS 5000 allows the sectioning of samples in two different modes In the single slice mode the unit prepares one section at a time and the blade repositions itself and waits to perform the next slice upon triggering by the user In the multiple slice mode the OTS 5000 will automatically perform multiple uniform slices The OTS 5000 control panel has been redesigned to allow for ambidextrous and ergonomical operation The keypad has been sealed to avoid exposure from buffer spills The tray is made from molded clear polycarbonate for improved viewing of the slicing operation The tray is removable to facilitate specimen mountin
103. red tip configuration Order Model W4 65 0207 MS 7PB Micropipettes Micropipette 7 Barrel 1 5mm OD Borosilicate Glass Pre Pulled Blank pkg of 20 Micropipette 7 Barrel 1 5mm OD Borosilicate Glass Fully Pulled approx 20 um Tip pkg of 10 W4 65 0208 MS 7MT Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Pipette Pullers A state of the art microprocessor controlled horizontal pipette puller designed to simultaneously pull two identical single barrel pipettes Needle micropipette fabrication devices include instruments intended to pull bevel or forge various types and styles of glass micropipette needles for ICSI IVF transgenics patch recording or other similar procedures involving the use of micropipettes in electrophysiological recording microinjection and microperfusion techniques The first step in the process involves the initial fabrication of the micropipette itself using either single or multibarrel capillary tubing This tubing typically ranges in size from between 1 and 2 mm OD and is usually made from borosilicate glass although other materials such as aluminosilicate or quartz are also used in some applications The capillary tubing is then placed in a micropipette puller where a point on the capillary tubing is brought to its softening point through the use of a heating device such as an electrical filament laser or gas flame Once the ca
104. ress Servic Road 01746 1388 USA e Requested A WARNER INSTR UMENTS A Harvard Apparatus Company 1125 Dixwell Avenue Hamden CT 06514 USA Phone 800 599 4203 toll free 203 776 0664 Fax 203 776 1278 Email support Qwarneronline com Publication W4 O Copyright Harvard Apparatus HARVARD APPARATUS 84 October Hill Road Holliston MA 01746 1388 USA Phone 800 272 2775 toll free 508 893 8999 Fax 508 429 5732 Email bioscience Qharvardapparatus com Presorted Bound Printed Matter U S Postage PAID Harvard Apparatus
105. ring bracket W4 72 6051 need to purchase separately The adjustable probe clamp allows firm hold of the widest range of probe and electrode diameters providing absolutely steadfast grip of any probe rod or electrode holder from to inches Rectangular base and mounting ring bracket must be purchased separately Specifications Axis Range Resolution X 65 mm 0 1 mm Y 29 mm 0 1 mm Z 65 mm 0 1 mm Z fine 2 2 mm 0 01 mm Weight without base 1 25 kg Order Product W4 72 6030 Right Handed Fixed Axis W4 72 6031 Right Handed Tilt Arm W4 72 6032 Left Handed Fixed Axis W4 72 6033 Left Handed Tilt Arm W4 72 6034 Rectangular Base W4 72 6051 Mounting Bracket Rectangular base not included Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com NEW High Resolution Motorized Micromanipulator The SM 3 is a high resolution three axes motorized micromanipulator featuring a compact design 25 nm resolution and a low noise controller Linear amplifiers used to drive the stepper motors eliminate stray electromagnetic radiation a key feature for patch clamp and noise sensitive electrophysiology applications The mechanical design of the SM 3 manipulator is compact and is optimized for direct attachment to a microscope stage The x slide may be tilted by 90 degrees making precise positioning of the tool assembly simple and direct Additionally an integral swivel devi
106. ropipettes from aluminosilicate glass Like silicon aluminum combines with oxygen to form Tetrahedral Networks and the Al O bonds are very strong In comparison with borosilicate glass aluminosilicate provides increased hardness improved chemical N N D gt KE o Aluminosilicate Capillaries with Filament durability reduced electrical conductivity and a lower coefficient of gt OD D tent ID Lenath thermal expansion Also while the original ratio of a borosilicate 2 Ge i g capillary S inner to outer diameter will remain unchanged over its oo Order mm mm mm Oty ee total taper length aluminosilicate glass demonstrates a marked W4 30 0110 10 08 HO gt tendency to thin out as it is drawn to a tip This behavior allows W4 30 0108 1 0 0 53 100 500 extremely fine tips to be formed W4 30 0109 1 0 0 53 150 500 For more information see 1 Na H Exchange Vaughan Jones RD Grinstein Press Ch 1 p 8 2 Effects of intracellular and extracellular pH on contraction in isolated mammalian cardiac muscle Bountra C amp Vaughan Jones R D Journal of Specifications Physiology Volume 418 1989 Composition 51 9 SiQ 22 0 Al 0 7 8 P 0 7 7 Mot 6 9 CaO 2 1 B 0 1 4 BaO and 0 2 As 0 Softening Temperature 950 C Dielectric Constant 6 2 Borosilicate Glass Rod Borosilicate Glass Rod OD Length Order mm mm Oty W4 30 0087 1 0 75 500 emm W4 30 0085 1 0 100 500 W4 30 0086 1 0 150
107. rrrrrrrerrrrrrrrrvrrrrvrrrerevererrrererrrrsrnnnsenn 299 Table of Contents Joystick Manipulator ccc cecccececscececscscscsesesesesesesesesscecscsssssssesesesesecacstscssseesseeeeees 300 Ultraprecise Micromanipulators rorrrrorrrorerorerororererrrrrrrrrrerererevereverrrrsrrrrnersverevennnn 301 Microdrive Controller Type Op4 302 DC Microdrive Controller Type 864 1 enrvrnrnrrvrrvrvrrrererererererenrvrnrrrnrrrrrrveveveverererenenn 303 DC Microdrive Controller Type OD4 7 303 Magnetic Bases 304 Microelectrode Holders E Series Electrode Holders 2 u nvmessesdtumennilnmninnmieddv bn dekedesn 305 310 O Series Electrode Holder 311 315 ME Series Electrode HOlpders omvvssoeesenadenssmserdednnesn 316 137 PE Series Electrode Holder 318 PE30W series holders 15 new models for patch perfusion soerrrorrrrrrrrverrrerrrrnnnn 319 MP Series Electrode Holders Segoe BEE 320 MHH 25 amp MHH 38 Holders ccs EES 321 Theta Glass Electrode Holder 321 THP Pressurized Holder for Theta Glass 322 Electrode Holders Replacement Hartz 323 Capillary Glass 2 2 324 330 Pipette E 331 333 MF 5 amp MG 5 Microforge Grinding Genter xsrsrvrnrnvnrnenrnrnvnrnenrnvnvnrnenenvnsnr 334 EMS Automatic Oscillating Tissue Slicer OTS 5000 335 336 Microscopy Section 339 TEE Model Z850 Stereo Zoom Microscope Gees 338 341 3025 Series Compound MIctoscope ssccciccecectosdevecesadeciscecedevxoterdaseehieseatcatedseati
108. ruction strong and durable e Available with fixed or tiltable X axis arm e Stable probe clamp allows for firm hold of 1 4 to V2 inch probe rod or electrode e Available for right or left handed use Manufactured from brass these Dovetail Slide Micromanipulators are significantly more durable and hold greater weight without slippage or drift than any ball bearing slide micromanipulator available Micromanipulators are available with a fixed or a tiltable X axis arm The fixed arm version has the X axis locked at precisely 90 The tilt arm version can be rotated to any desired angle and securely locked The dovetail slides are driven by rack and pinion mechanisms for rapid positioning to a resolution of 0 1 mm on the coarse vertical Z and lateral Y drives The probe direction X drive is also graduated in 0 1 mm increments and has an anti backlash worm drive for more precise control of movement The fine drive graduated in 0 01 mm increments is located on the base of the Z axis This is especially useful where the specimen is in a Petri dish or microtiter tray The probe holder allows mounting of any rod or pipette even up to inch diameter These micromanipulators can be mounted directly on several bases via the most secure dovetail slide mount Alternatively a mounting ring bracket is available which allows mounting onto any 7 inch rod Electrode Holder not included Retangular base W4 72 6034 and 10c mounting
109. rvrvrrrrrrerrrrrrrrrrennnn 89 VC 6 amp VC 6M Valve Control Systems oo ccc ccecscecececesesesesesescetseetecseeeseeeeees 90 91 VES Valve TIE lt socnsoiscsesensscchsosetsssuecssdhackavexsicnes eege 92 Valve Control Systems Parts and Accessories 0 0 1ssissisesiesieeieisienienreeieirninenrenn 93 Fast Step LE 94 96 Accessories Syringe Holders MSH SH 97 Constant Flow Syringes DN Gees 98 Gas Bubble Manifold GBM10 amp GBMG60 ou ccc ce cecececeteceteneseseseseetsestseeteeaes 99 Tubing connector Kits Syringe Needles rorrrrnrrrrrrvrrrvrvrvereveverrrrrersrrrrrrnverereenennn 100 Manifolds and Control Hardware ML MM MP amp MPP ees 101 Electrode Manifold Holders MHH 25 amp MH 209 102 Vacuum Solution Flow Valves FR 50 SPD 102 Gas OMEGA 103 104 Spil Sensor Systems iirinn aaisa 105 106 Temperature Control Section 107 Controllers Chamber System Temperature Controllers TC 324B amp TC 344B ou 108 109 Bipolar Temperature Controller CL 100 sornorrrrnrrrrrrrrrrrrrrrerererereverererrrrrrrerrrrnrnvennnn 110 Bipolar Temperature Controller 200 111 Model LCS 1 Liquid Cooling Systems 112 Bipolar Temperature Controller TC 20328 113 Temperature Controller TC 124A eorovrvrrrrrnrrerenrvrvrvrrrererererererererrsrrersrsrrsseveverevenennn 114 Dual Temperature Controller TC 144 nmemnenenenveveveverererererrvrrrssrsrrernsevevevererererennnnnn 115 Warmed Platforms WP 10 amp WRIp 116 In Line
110. s RUSSIA Science Pribor 23 15 Avtozavodskaya str Moscow Russia 115280 email orders sciencepribor ru website www sciencepribor ru SINGAPORE C Prime Bioscience Pte Ltd Blk 431 Clementi Avenue 3 01 358 Singapore 120431 phone 65 6364 0391 fax 65 6269 0745 e mail info primebioscience com SOUTH KOREA Sang Chung Commercial Co Ltd Dae Kyung Bldg 3F 839 15 Yuksam Dong Kangnam Ku Seoul Korea 35 935 CPO Box 1072 phone 82 2 564 8766 fax 82 2 561 1603 e mail info sang chung co kr website www sang chung co kr Scitech Korea Room 302 Kangbuk Union Building Beon 3 dong Kangbuk Gu Seoul 142 706 Korea phone 82 2 986 4419 fax 82 2 986 4429 e mail scitech00Gscitechkorea co kr GERMAN SWITZERLAND Hugo Sachs Eletronik Harvard Apparatus GmbH Gruenstrasse 1 D 79232 March Hugstetten phone 49 7665 92000 fax 49 7665 920090 e mail info hugo sachs de TAIWAN Major Instruments Co Ltd 9th Floor No 69 3 Chung Cheng E Rd Sec 2 Tan Shui Taipei Taiwan ROC phone 02 2808 1452 fax 02 2808 2354 e mail majortw ms6 hinet net website major major com tw TURKEY Commat Cetin Emec Blv 74 Sk No 4 9 Ovecler Ankara 6460 Turkey phone 90 312 472 74 17 fax 90 312 472 74 18 e mail info commat com fr uonegnouy Abojors yd siaquiey 0499 3 CHA E lt uone1odonaa g buisuasoig saxapul Harvard Apparatus 84 October Hill Holliston MA Add
111. se Holding Hose and Input Hose Adapter PLI 100A Plus Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Foot Switch 65 0029 Pipette Holder 65 0013 and Input Hose Adapter PLI 100A Deluxe Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Two Foot Switches Two Pipette Holders and Input Hose Adapter tian 5 tl Ol ar V U Proini GIUN j mii gt 5 o an de uondafuio1du o sce z e 280 PLI 90 Medical Systems Pico Injector Precise and reproducible injection W4 65 0004 PLI 90 Pico Injector e 3 pressures inject balance and clearing e Eliminates backflow into pipette after injection e Reproducibly delivers femtoliters to microliters e Easy to use e Economical e Precise The PLI 90 Pico Injector like the reliable PLI 100 Pico Injector controls the precise and reproducible regulation of injection pressure and time Simplicity Because the PLI 90 features only the injection balance and clearing pressures It Is a lower cost alternative to the PLI 100 This simplicity makes it even easier to use than the PLI 100 Selection Guidelines The PLI 90 is ideal for the user who does not require vacuum for filling pipette barrels or if using a holding pipette for holding cells is not essential to your applica
112. sides programmable timers and counters there are 16 programmable and savable injection perfusion sequences with 32 programmable steps in each sequence Each sequence can be repeated continuously or triggered manually The interval time between steps can be programmed for automatic sequential cycle or manual trigger There are two foot switch connectors for sequential step trigger and injection trigger A digital remote port can be connected to an optional remote key pad or be controlled by a computer With exceptional versatility and extremely precise control either the PM 4 or 8 is an ideal multi channel injection perfusion engine Connection Diagram for Extracellular Micro Perfusion and Intracellular Micro Injection Systems PM 4 8 Programmable Pressure Injector 30 to 100 psi Pressure Source PM4 4 Channe Injection PMB 8 Channel Injection Pressure Output Drain Out Liquid OR PP 8 i 7 Barrel Pipette J Y KS a EZ Hold Cell Pipette cuth UU UH Pipette Il Single Barrel Injection Pipette Tested Cell Extracellular Micro Perfusion Connection Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com N AL FSW Foot Switch CC PM KP Remote Injection Time Signals Key Pad of 4 or 8 Channels A Barel Pipete SE AANS nin Hold Cell Pipette angle parel Injection Pipette Intracellular Micro Injection Connection microi
113. silicate Double and Triple Barrel Specials e Easy filling e Available in standard or thin wall configurations with selection of three outside diameters e Exceptionally low tip impedance less than 50 MQ e High quality borosilicate glass Good for patch clamp pipettes and microinjection Special two barrels one barrel with filament needles one without e Ratio of outside to inside diameter preserved Borosilicate with Filament to tip during pull OD ID Length _ i i Order amp mm mm mm Qty Borosilicate Double and Triple Barrel Specials Borosilicate Standard Wall with Filament OD ID Length W4 30 0034 1 0 0 50 75 500 OE a an Qty W4 30 0032 1 0 50 100 500 Double Barrel S W4 30 0033 1 0 0 50 150 500 W4 30 0004 1 5 0 86 15 100 W4 30 0021 1 0 0 58 75 500 W4 30 0005 1 5 0 86 100 100 5 W4 30 0019 1 0 0 58 100 500 W4 30 0006 1 5 0 86 150 100 W4 30 0020 1 0 0 58 150 500 Triple Barrel amp W4 30 0046 1 2 0 69 75 350 W4 30 0011 1 5 0 86 15 100 W4 30 0044 1 2 0 69 100 350 W4 30 0009 1 5 0 86 100 100 W4 30 0045 12 0 69 150 350 W4 30 0010 1 5 0 86 150 100 Li Se Se 2 Borosilicate Theta Glass W4 30 0058 1 5 0 86 150 225 W4 30 0076 2 0 1 16 75 125 W4 30 0074 2 0 1 16 100 125 W4 30 0075 2 0 1 16 150 125 Easy to fill W4 30 0084 3 0 1 62 15 55 i i e sene SG Ces we 5 e Two channels in a single diameter W4 30 0083 3 0 1 62 150 99 Borosilicate Theta Glass Borosilicate Thin Wall with Filament OD ID Septum Length W4 30 0040 1 0
114. sion technique patch clamp electrodes J Physiology 32 195 210 2 Pusch M and Nehr E 1987 Kinetics of loading small cells with various compounds by use of patch pipettes Pflugers Archives Spring Meeting of the Physiol Ges Tang J M Wang J and Eisenberg R S 1992 Perfusing patch pipettes Methods in Enzymol 207 176 181 E PE30W SERIES Holders Glass S Order Wire Pellet Body Style Port Connector OD mm Model W4 64 1761 Wire Straight Yes 1mm Pin 1 0 PE30W A10P W4 64 1762 1 2 PE30W A12P W4 64 1763 15 PE30W A15P W4 64 1764 1 7 PE30W A17P W4 64 1765 2 0 PE30W A20P W4 64 1766 BNC 1 0 PE30W B10P W4 64 1767 1 2 PE30W B12P W4 64 1768 15 PE30W B15P W4 64 1769 1 7 PE30W B17P W4 64 1770 2 0 PE30W B20P W4 64 1771 1 mm pin 1 0 PE30W T10P W4 64 1772 Threaded 1 2 PE30W T12P W4 64 1773 Collar 1 5 PE30W T15P W4 64 1774 1 7 PE30W T17 W4 64 1775 2 0 PE30W T20P Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 319 VIP Series MIP Series Electrode Holders Microelectrode holders for every application and manufacturer Pressure Ports Accepts 1 5 mm tubing B Accepts 2 4 mm tubing ML Accepts female Luer fitting FL Accepts male Luer fitting T Accepts 4 28 male thread fitting Specifications Body Material Acrylic MP SxxT Body Style Straight Port Ys barbed Die tubing ID barbed tubing ID mal
115. specialized tools for Electrophysiology amp Cell Biology Research Planar Lipid Bilayer Perfusion Microfluidics Oocyte Clamps Patch Clamps Microinjectors Microincubators Micromanipulators Ussing Diffusion Systems Live Cell Imaging Chambers Temperature Control Systems BTX Electroporation Transfection Call to receive other catalogs of interest WARNER eg A physiology amp Animal Behavioral INSTRU M ENTS Cell Bi Organ amp Cell Research A Harvard Apparatus Company 1 Physiology Welcome to the Electrophysiolot WARNER A INSTRUMENTS A Harvard Apparatus Company Dear Researcher Warner Instruments is proud to introduce our new Electrophysiology amp Cell Biology Catalog This catalog contains many new products for cell imaging biosensing microinjection and electrophysiology NEYV Products Featured Include PLI 100A Picoliter Microinjector VVith three positive and two negative pressure capabilities the versatile PLI 100A is capable of large injections into capillaries or small injections into mammalian nuclei e BioStat Multi channel Potentiostat The BioStat is a software driven multi mode potentiostat that can be used for measurement of pH reactive oxygen species and nitric oxide Compact Motorized Micromanipulator Linear amplifiers used to drive the stepper motors eliminate stray electromagnetic radiation reducing noise and resulting in improved pa
116. t with a minimum of hand transmitted vibration backlash or drift The instrument can be used alone or can serve as an ideal mount for even more precise microdrives For ultimate precision the Ultraprecise Motorized Control Micromanipulator This ultraprecise motorized control micromanipulator is offered either with stepper motors on two axes or on all three axes These motors are controlled by a precision controller that is supplied with the instrument The same micrometers are used as with the manual version with a travel of 25 mm 1 in The controller provides fast motor speed of 9 revolutions sec which allows a maximum velocity of 36 mm sec Speed is infinitely variable to a slowest speed of 40 000 steps per revolution which corresponds to 0 01 um per step Only by these extremely small single steps can an operator be assured of essentially vibration free movement The controller works in a vector mode that is the target coordinates are approached directly in a Straight line from the starting point In all modes there are end stop switches The movement can be programmed on a thumb wheel switch and is started by a press button Alternatively advance and retraction may be controlled by manual operation of buttons A return button causes the probe to be retracted to the zero position The Motorized Controller is available with or without a display The Controller with Display monitors the location of the probe on a six digit LED counter
117. tart stop trigger gate 1 through 5 banana jacks terminals floating input optically coupled input voltage 5 to 15 V TTL compatible Lemo miniature receptacle ground referenced 5 mV nA input impedance 100 kQ microinjection Lemo miniature receptacle 5 mV nA ground referenced Lemo miniature receptacle TTL pulse 7 pin miniature connector mates with ultra flexible cable leading to micro electrode holder 21 x 47 x 35 cm 8 75 x 19 x 14 in 8 2 kg 18 Ib gt D iz an T GA 125 V at 0 1 A 15 V at 0 5 A 5Vat3A Line operated 115 to 220 VAC 50 60 Hz 13 x 47 x 35 cm 5 1 4 x 19 x 14 in 11 kg 24 Ib 285 Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection continued Specifications Cycle Start Cycle Stop Push button and banana jack terminals Trigger Gate Inputs floating input optically coupled Input Voltage 5 to 15 V Minimum Trigger 5 usec at5 V Pulse Width Sync Out Lemo miniature connector TTL pulse Single Cycle Mode In single cycle mode start switch or external trigger initiates each cycle Recycle Mode In recycle mode once start switch or and external trigger is actuated repetitive cycles will continue until stopped Time Unit Switch Two basic time units can be selected 10 msec or 1 sec In 10 msec position Eject and Pause time switches of PPM 2 Modules can be set to cover time range from 10 to 990 msec with 10 msec resolution In 1 sec positio
118. tch clamp and electrophysiology performance PFC 1 Proflow Chamber Computer designed gaskets optimized for well defined well controlled shear flow e RC 49FS Perfusion Chamber with Field Stimulation Uses popular 18 mm round coverslip The low profile design allows for low entry angle patch electrodes e CL 200 Dual Channel Bipolar Temperature Controller Single control temperature pt adjustment built in protection for Peltier devices open thermistor fault protection e New Zoom Stereo Microscopes Versatile high performance ergonomically designed microscopes with multiple stand options B ProgRes Microscope Cameras These CMOS and CCD cameras are suitable for all contrast methods in light microscopy C Mount and USB2 0 FireWire interfaces All ProgRes cameras include CapturePro image capture software sincerely bh ST Ralph Abate Business Manager Warner Instruments WEI CL 200 Dual Channel Bipolar Temperature Controller p 111 ee Perfusion E Chamber with Field Stimulation p 54 A 4 PFC 1ProfloW o x Chamber p 57 N oe TE 0 BioStat i Multi channel Potentiostat p 372 u Compact Motorized Micromanipulator p 297 ProgRes Microscope be Cameras p 344 New Zoom Stereo Microscopes p 338 e Warneronline com PLI 100A Picoliter Microinjector p 278 See more products on our v Table of Contents
119. ted handle Package of 10 Flexible Fill Capillary Tubes 450p 0 D Package of 10 Feet of 1 16 Tygon tubing Hot Glue Gun Hot Glue Sticks Package of male luer to 1 16 barb fittings 25 pcs pkg Package of 2 0 mm 0 D Theta glass 100 pcs pkg Package of 10 Flexible Fill Capillary Tubes 350u 0 D 0 D Package of 10 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Electrode Holder Replacement Parts Microelectrode Replacement Parts Order Oty Pkg Model Description Coupling Elements W4 64 1297 3 SP 1M Molded Ag AgCI pellet assembly A W4 64 1282 10 AG25 10 Ag wire 0 25 mm diameter x 70 mm long 1 mm W4 64 1319 AG10W Ag wire 0 25 mm diameter x 2 m 6 6 W4 64 1288 1 HP 205 Hybrid Ag AgCI pellet Ag wire assembly with 40 mm teflon sleeve sealed at junction of pellet and silver wire Pipette and Wire Seals W4 64 1289 10 PS 10 Pipette seal for 1 0 mm diameter glass W4 64 1290 10 PS 12 Pipette seal for 1 2 mm diameter glass W4 64 1291 10 PS 15 Pipette seal for 1 5 mm diameter glass W4 64 1374 10 PS 17 Pipette seal for 1 7 mm diameter glass Paa Pike W4 64 1292 10 PS 20 Pipette seal for 2 0 mm diameter glass W4 64 1298 10 WS 1 Wire seal for E ME MP and theta holder A 9 W4 64 1299 10 WS 2 Wire seal for Q and PE Series holders W4 64 1300 10 WS 3 Wire seal for Q and PE Series holders with WS 2 WS 3 threaded T connector 3mm OD 2mm OD E
120. th Handle Glass Order Wire Pellet Connector Port OD mm Model W4 64 1023 Pellet 2 mm Jack No 1 0 E45P FIONH W4 64 1024 1 2 E45P F12NH 5 W4 64 1025 1 5 E45P F15NH S W4 64 1033 2 mm Pin 1 5 E45P M15NH W4 64 1026 2 0 F45P F20NH W4 64 1039 Yes 1 0 E45P F10PH W4 64 1040 1 2 E45P F12PH I W4 64 1041 1 5 E45P F15PH S W4 64 1042 2 0 E45P F20PH a W464 1019 Wire 2mmdJack No 1 0 E45W FIONH S W4 64 1020 1 2 E45W F12NH D W4 64 1021 1 5 E45W F15NH W4 64 1022 2 0 E45W F20NH S W4 64 1035 Yes 1 0 E45W F10PH Ce W4 64 1036 1 2 E45W F12PH W4 64 1037 1 5 E45W F15PH W4 64 1038 2 0 E45W F20PH W4 64 1051 Vent 1 0 E45W F10VH W4 64 1052 1 2 E45W F12VH W4 64 1053 1 5 E45W F15VH W4 64 1372 17 E45W F17VH W4 64 1054 2 0 E45W F20VH Vented models are standard with the Warner OC 725 Oocyte Clamp 310 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Q Series Q Series Electrode Holders QSW Bxxp QSW TxxP QRW AxxN Q45W AxxP How to Decode Q Series Model Numbers Body Style S straight M right angle 90 45 45 T 1 mm pin withreaded collar DENNEN sf Connector F 2 mm jack M 2mm pin A 1mm pin B BNC QSW MxxP e Patch Clamp Recording e Intracellular Recording Coupling Glass Size Port Vent W Ag Wire 10 1 0 mm N no port or vent Specifications W Hybrid 12 1 2mm P
121. tion The PLI 90 is available in two packages Basic and Plus The basic model includes an input and output hose handle power cord and instruction manual The plus model is supplied with all of the same parts as the basic model but also includes a Footswitch Pipette Holder and Input Hose Adapter Specifications Input Gas Pressure Injection Pressure Balance Pressure Clearing Pressure Injection Time Pressure Display Duration Mode Trigger Mode Pressure Readout Line Voltage Power Usage Foot Switch es Accessories Supplied Weight Dimensions H x W x D Order Model 70 to 105 p s i 480 to 720 kPa 0 2 to 60 p s i 413 kPa regulated multi turn control 0 1 to 9 9 p s i 68 9 kPa regulated multi turn control other ranges available upon request Input gas pressure unregulated 0 01 to 0 99 sec in 10 msec steps 1 to 99 sec in 1 sec steps Digital three and a half digits Internally timed or externally triggered Foot or panel switch Inject balance clear output port 100 110 220 240 VAC 220 W Optional inject and gating Input hose output hose and power cord 6 8 kg 15 Ib 11 x 38 x 25 5 x cm 5 x 15 x 10 in Product W4 65 0004 PLI 90 BASIC Pico Injector with Injection W4 65 0005 PLI 90 PLUS Balance and Clear Pressures Includes Input Hose PLI IHN and Output Hose PLI OHN Handle Power Cord and Instruction Manual Applications Basic setups that do not need suction Pico I
122. tor turns but the micromanipulator does not move For use on either 115 230 VAC 50 60 Hz Order Product W4 60 0577 Push Button Control Unit E m L IA J EE Sy val d z KE KR Ge ee ee de Ve NN e E GE SE SE Ge EE Ge KEES GREEN deet EE 3 Direction Joystick Control Unit e Provides simultaneous movement in three axes e Permits easy placement of probe within accuracy of 2 or 3 um This control unit permits control of movement in three directions simultaneously The speed and direction of forward or backward movement is regulated by the degree of displacement of the joystick from the central null position The third axis of simultaneous movement is obtained by rotating the joystick handle For use on either 115 230 VAC 50 60 Hz Supplied with a screened cable Joystick requires W4 60 0577 for operation Order Product W4 60 0581 3 Direction Joystick Control Unit W4 60 0580 Replacement Screened Cable W4 60 0604 Tool Holder pkg of 3 W4 69 1066 10 mm Rod Clamp for use with Magnetic Bases W4 69 1067 12 mm Rod Clamp for use with Magnetic Bases Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com micromanipulators gt D Ki an T 299 SJoJe ndiueuosoIu CHA d Micromanipulator Joystick Simple multi dimensional control e Joystick reduction gear ratio from 1 15 to 1 150 e Fine positioning on Z
123. traight Jack 6 3 mm diameter x 6 3 cm long threaded to attach to Connector ve barb barb male Luer female Luer threaded 4 28 Ys barb MEW FxxT MEW FxxFL Glass OD mm 1 0 1 2 1 5 2 0 1 0 1 2 15 2 0 1 0 1 2 1 5 2 0 1 0 1 2 1 5 2 0 1 0 1 2 L 2 0 1 0 1 2 L 2 0 Model MEW F10A MEW F12A MEW F15A MEW F20A MEW F10B MEW F12B MEW F15B MEW F20B MEW F10ML MEW F12ML MEW F15ML MEW F20ML MEW F10FL MEW F12FL MEW F15FL MEW F20FL MEW F10T MEW F12T MEW F15T MEW F20T MEP F10A MEP F12A MEP F15A MEP F20A 10 16 Thread 10 32 Thread Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com ME Series ME Series Electrode Holders continued ME SERIES Holders Order W4 64 1184 W4 64 1185 W4 64 1186 W4 64 1187 W4 64 1192 W4 64 1193 W4 64 1194 W4 64 1195 W4 64 1188 W4 64 1189 W4 64 1190 W4 64 1191 W4 64 1196 W4 64 1197 W4 64 1198 W4 64 1199 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Wire Pellet Body Style Port Connector 2 barb male Luer female Luer threaded 28 Glass OD mm 1 0 1 2 1 5 2 0 1 0 1 2 L 2 0 1 0 1 2 1 5 2 0 1 0 1 2 15 2 0 Model MEP F10B MEP F12B MEP F15B MEP F20B MEP F10ML MEP F12ML MEP F15ML MEP F20ML
124. uding SC 02 Coupling W4 65 0215 Model OC 01 Output Cable 2 7 m 9 ft with 7 Seven Pin Lemo Connector W4 65 0285 Model NL 952 Cable 2 m 6 5 ft with Lemo Miniature Connector at One End and Tinned Leads at Other Sync Output or Analog Input Output Cable 286 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection continued IP 2 lontophoresis Pump Module Each IP 2 Module includes controls for precise settings of current magnitude and polarity retention 0 to 50 nanoamperes ejection 0 to 500 nanoamperes The actual current and polarity is continuously displayed digitally and can be externally monitored at the analog output terminal Operating Modes Ejection Timing and Mode Switch The mode switch provides five push button controls which include operations such as cycle trigger gate continuous and termination Cycle Mode In the cycle mode by virtue of selecting single or recycle operation on the BM 2 Module an incoming trigger or cycle start push button will initiate the current ejection pumping action In this mode each succeeding Pump Module is automatically triggered after the pause time of the preceding event has been completed Both eject and pause times can be preset to cover a range from 10 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second res
125. uoted in US dollars only and are for shipment and use within the US only Prices listed do not include shipping charges Shipping charges are prepaid and added to invoice at time of shipment Section Page No Microinjection Introduction to Microinjection 214 Pico Injection Micromanipulation System PLI 10 275 Pico Injector Systems PLI 100 8 216 277 Pico Injector Systems PLI 100A 278 279 Pico Injector Systems PLI 90 280 PLI Injector Accessories 281 PLS 1 Pico Injection Micromanipulation System 282 Air 1 Ultra Low Noise Air Compressor 283 BH 2 Neurophore _ 284 288 Nanoject Auto Nanoliter Injector 289 Screw Actuated Air Syringes 290 Screw Actuated Micrometer Driven l Hamilton Syringe 290 PM 8 amp PM 4 Multi Port Pneumatic E Injection System 4 8 Channel 291 292 Micropositioning i Micromanipulator Selection Guide 293 294 Standard Manual Control Manipulators 295 Dovetail Micromanipulators 296 High Resolution Motorized Micromanipulator SM 3 297
126. utomatic feedback and control of inverse sum of all pump currents the balance module has independent capability of current pump settings with a working range of 0 to 500 nanoamperes The Balance Module includes a digital display time clock provisions for electrical and manual cycle start stop and single cycle recycle switch trigger and gate input terminals to initiate externally controlled eject pumping action of respective modules Analog input for balance or drive with override capability Analog output for monitoring of unbalance currents Brain Slice Chambers are available in a variety of formats Please visit our website for more information Order Product W4 65 0600 BH 2 Mainframe Chassis Does Not Include BM 2 Balance Module see Below W4 65 0602 BM 2 Control and Balance Module with OC 01 and NL 952 Application Note Working Unit One 1 each of BH 2 Main Frame MS 2 power supply BM 2 Control amp Balance module and any combination of one 1 to five 5 modules selected from IP 2 and or PPM 2 If less than five 5 DP 1 module is required to fill the spaces MS 2 Power Supply The AC power supply is self contained in a rack mounted cabinet and provides all voltages required to operate the Neuro Phore System The power supply interconnects with the mainframe via flexible cable The supply works with 115 or 220 VAC 50 60 Hz mains source Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 420
127. verenrrrnrrrnrrrrreveverevevererennnn 407 Generator Specifications ett 408 409 Enhancer UNE 410 Cuvettes E EE 411 BTXpress High Performance Electroporation Solutions 412 413 Specialty Electrodes Denetrodes oe cececececscecececssesesssessssscsessasasevavasasesssesessssssssecscssseecasasaueseeeseseseseieiseeetseaes 414 DEER EE tee 415 E 415 2 Needles Amay 416 Caliper Electrodes oorrrrrororerrrrrrrrnvrrrrvevevevererererensvrvrnsrrrsrerevevevevsvenevenersrerersrrrrsnnsnnn 416 Flatpack Chambers mueseusnmermnsengassminebeidesni gulv jhulvelndedujne 417 Fat Electrode er 417 FT ee 418 Petri Dish Electrode es sneeeensinnssnenssinsisenssnsisansnnsnrnnssinnnrnsnnnrsnrnnnnrnnrinrenrnnrerenrenrn 418 MN 2 419 Meander Fusion Lhamfber 419 Platinum Needle L Shaped Electrode rovrvrrrvrrererrrrrererrrrrrrrrvevernrerevevererrrerersrrnnsnnnn 420 Petri Dish Platinum Electrode for Tresugs 421 Petri Dish Platinum Electrode for Tissue lees 422 Perfusion Microfluidics Syringe amp Peristaltic Pumps Valve Control Systems Pressurized Perfusion Fast Step Perfusion Systems Gas Controllers Spill Detection Systems amp Accessories Temperature Control Low Noise Temperature Controllers In Line Solution Heaters and Coolers Objective and Syringe Warmers Thermistors Cables and Accessories Electrophysiology Equipment Patch Clamp Equipment Bilayer Workstation Oocyte Clamps Amplifiers Low Pass Filters Neurolog
128. with 6 3 or 9 5 mm diameters The holder head can be pivoted 90 from the axial position and rotated 360 about the axis Friction holds the head firmly in the set position Holder head EES GE and coupler are made from Delrin The anodized aluminum handle is 6 3 mm Manifolds N Ben ad 5 ad EZ E K Oo lt b Bsa S diameter x 6 3 cm long and will fit most positioners gt 2 ae co Specifications 3 Body Material Acrylic Body Style Straight Coupling Ag wire 2 Connector 2mm jack Port 2 mm diameter polycarbonate li Standard Glass Sizes 1 5 and 2 0 mm Theta Glass Holders Handle Nl diameter x 6 3 cm long threaded to attach to e Microinjection or microiontophoresis with theta glass Theta Glass Electrode Holders Glass Order Port Handle OD mm Model W4 64 1164 No No 1 5 THS F15 W4 64 1165 2 0 THS F20 W4 64 1172 Yes 1 5 THS F15H W4 64 1173 2 0 THS F20H W4 64 1168 Yes No 15 THS F15P W4 64 1169 2 0 THS F20P W4 64 1176 Yes 1 5 THS F15PH W4 64 1177 2 0 THS F20PH Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 321 S19p OY 3P01 23J30421U o se z e L 322 THP Pressurized Holder for Theta Glass This holder allows quick and easy coupling of theta tubing to a pressurized source Adhesive bond Theta Glass e Allows microinjection using theta glass Kit includes everything ne
129. with port l Straight 15 1 5 mm V with vent Body Material Polycarbonate 6 3 mm diameter Body Only 17 1 7 mm Body Styles Straight 45 and 90 20 2 0 mm Connectors 1 mm Pin 1 mm Pin Threaded and BNC Coupling Ag Wire or Ag AgCI Hybrid Port 2 mm diameter Polycarbonate Connector and Amplifier Headstage Compatibility Standard Glass Sizes 1 0 1 2 1 5 1 7 and 2 0 mm Connector A 1 mm pin B BNC F 2 mm jack M 2 mm pin Headstage Warner PC 505A B PC 501A Axon Patch Clamps prior to 2 1 95 List and Dagan Warner PC 501 amp Electrometers Axon Axoclamp amp Axoprobe prior to 2 1 95 T 1 mm pin threaded All Axon after 2 1 95 Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 311 O Series Q Series Electrode Holders with 1 mm Pin Compatible with Warner PC 501 and PC 505 Patch Clamps and Axon Patch Clamps prior to 2 1 95 NOTE Holders without ports are not suitable for patch clamp recording Q SERIES Holders with 1 mm Pin Glass Order Wire Pellet Connector Port OD mm Model W4 64 1105 Wire Straight No 1 0 OSW A10N W4 64 1106 12 QSW A12N W4 64 1107 1 5 QSW A15N W4 64 0745 1 7 QSW A17N W4 64 1108 2 0 QSW A20N W4 64 0821 Yes 1 0 QSW A10P W4 64 0822 1 2 OSW A12P
130. xe pkgs 6 8 kg 15 Ib 11 x 38 x 25 5 cm 5 x 15 x 10 in Input output and holding hoses microinjection Product PLI 100 Basic Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord and Instruction Manual PLI 100 Plus Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Foot Switch W4 65 0029 Pipette Holder W4 65 0013 and Input Hose Adapter gt D iz an T GA PLI 100 Deluxe PLI 100 Deluxe Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Two Foot Switches Two Pipette Holders and Input Hose Adapter Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 277 uondafurosdnu o se z e 218 PLI 100A Pico Injector ee e a am z wm mp g s MF Batz e KH H E e Femtoliter to microliter injections e Digital readouts for injection pressure time and count e Reliable optically encoded circuit for injection time set e 5 pressures inject balance clear fill and hold The PLI 100A Pico Injector reliably delivers ejections from femtoliters to nanoliters through micropipettes by applying a regulated pressure fo
131. y Speed 92 nl sec Volume Range 2 3 nl to 69 0 nl Change Volume Dip switches 1 to 4 E Change Inject Speed Dip switch 5 2 Weight 99 2 g 3 5 02 Order Product W4 69 0130 Nanoject II Nanoliter Injector 115 V 60 Hz US Plug W4 69 0131 Nanoject II Nanoliter Injector 230 V 50 Hz European Plug W4 69 0132 Nanoject II Nanoliter Injector 230 V 50 Hz UK Plug Replacement Parts W4 69 0133 Replacement Glass 88 9 x 1 14 x 0 053 mm 3 5 x 0 045 x 0 021 in L x OD x ID Vial of 100 W4 69 0134 Replacement Glass 177 8 x 1 14 x 0 053 mm 7 x 0 045 x 0 021 in L x OD x ID Vial of 100 W4 69 0135 Flared Glass 90 mm 3 5 in Vial of 100 W4 69 0136 Standard O Ring W4 69 0137 Special Flared O Ring W4 69 0138 Replacement Wire Plunger W4 69 0139 Needle 30 ga x 51 mm 2 in W4 69 0142 Universal Mounting Adapter see above photo Accessories Must be ordered separately W4 69 0140 Footswitch W4 69 0141 Support Base Universal adapter now included for all MIZ Warner Instruments e Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 e www warneronline com 289 uondafurosdnu o z e 290 Screw Actuated Syringes Screw Actuated Air Syringes e For air assisted microinjection and microaspiration e Uncomplicated and easy to use e Accurate e Sensitive control e High suction pressure These air assisted microinjection microaspiration syringes provide the user with sensitiv

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