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1. Set the step size in um for the autofocus movement Engine Allows you to select the device used for the autofocusing Auto The software select automatically between the Z motor and the piezo following the size of the movement defined by the From and To parameters Z motor Force the software to use the Z motor for autofocussing Piezo Force the software to use the piezo for autofocussing LabSpec5 manual 30 HORIBAJOBIN YVON Z motor amp piezo The software uses the both devices for autofocusing A first approach Is done with the Z motor The research of the final position is then refined by using the plezo Intensity Display the autofocus diode intensity Validate the settings and close the Autofocus window Cancel Close the Autofocus window without validating the setting 4 4 9 Video functions Laser Position The Laser Position window allows you to modify the position of the laser beam on the video image These parameters are used when the XY scanning is used during the acguisition process To access this window run the video preview and then select Laser item in the Acguisition Video menu Then you can click and drug the laser position indicator with the mouse and the current position is refreshed in the CenterX and Center Y field It is also possible to enter the position directly in the CenterX Center Y through the keyboard Click the OK button to validate the settings
2. Save acquired data Select this box to save automatically the data when the acquisition process is finished Format arcos Select here the file format The list of the different format supported is the same one as for Open Save functions Folder Edit here the path where to save the files Click on the E button to select the folder in the tree structure buc Select this box to add the year number to the folder name Mont Select this box to add the month number to the folder name Da ssasnn Select this box to add the day number to the folder name Pile Pn Edit here the file prefix name OU aaa Select this box to add the hour to the file name MIN tr Select this box to add the minute to the file name AT Select this box to add the counter value to the file name The counter is modified each time the auto save function is called But you can edit the start value of the counter seguence LabSpec5 manual 26 HORIBAJOBIN YVON 4 4 6 Multi window function The Multi Window function allows to set the properties of the acguisition in multi window mode This mode enables to record data automatically over an extended range with a defined integration time and averaging The extended range is covered by taking a number of individual single shot windows and gluing these together To acces this Dialog select Multi Window item in the Acguisition menu
3. o LabSpec Spectrum d03 The main menu bar File Edit Options Maintenance Acquisition Data Window Help SSH Swill us ig k v mg The main icons bar Spectrum d03 ET 307 Graphics tools associated to the active object 204 Intensity cnt PHS Mite JOSTEEES 500 1000 oun oe Raman Shift cra km The control panel The status bar For Help press F1 i amp Y DT vr dH D ta t ja 1500 2000 Ve Window of the active objects 099 095 562 LabSpec5 manual HORIBAJOBIN YVON 4 The main menu bar The main menu bar is located on top of the LabSpec5 main window Lab5pec Spectrum Chalcapyrite1 File Edit Data Acquisition Options Setup Window Help 4 1 File menu The File commands menu permits to manage the data files and the data printout To access the File menu click on the File button of the main menu bar or type Alt f or Alt F keys Open Ctrl 0 Data file management functions Close Save Ctrl4 5 Save All Print Ctrl F Data printout management functions Print Preview Print Setup Page 1 xYImage benzen 1103 30 10 tvf Last loaded files p 2 Video porki TYF 3xYImage porki 1151 126 140 TVF 4 CASA Vires rad x40 325 tsf Exit gt Data file management functions OPM Re To open a previously saved object for
4. Fitting Iteratian To select and define peaks manually use the following icons on the left graphics tool panel X To add or move a peak be PoR PA To adjust the height and width of a peak EE E To remove a peak Adding or moving a peak f Spectrum d03 sooo bd Click on this 1con 7000 Ou mu Then move the cross at the desired position A peak will appear in function of the settings of the SHOW properties A ca c Intensity cnt 5 T LabSpec5 manual 50 HORIBAJOBIN YVON Adjusting peak height and width x Click on this icon to define or modify the height and width of the selected peak Peak height modification Move the mouse on the height of the peak to modify When the following symbol appears click and drag the mouse to the wished height Peak width modification Move the mouse on one of the sides of the peak to modify When the following symbol yl or E appears click and drag the mouse to obtain the wished width Removing a peak X Click on this icon to remove peaks Spectrum d03 Move the mouse pointer on the peak you wish to remove When the following x symbol O appears click on the left mouse button The correponding peakk Is removed OS EFFES Intensity ent AE LIE T 41 ur re N SEARCH function The Peak Search operation performs a search for peaks It iterates
5. This parameter is used for a non 3D look Main Grid click on the control to modify the color and style of the main grid Sub Grid click on the control to modify the color and style of the sub grid Values click on the control to modify the values font Labels click on the control to modify the labels font Ticks click on the control to modify the color and style of the ticks 3D Look Check this box to enable a 3D outlook for the edge 8 5 Graph This function allows you to customize the appearance of 1D dimension displayed data objects Spectrum or Spectral Image 8 5 1 Spectrum If the active object 1s a spectrum selecting Graph item of the window context menu opens the spectrum Properties dialog which allows you to modify the display properties of the spectrum Select one of the Style controls to display spectrum as a set of Lines Spectrum Options Points or Bars RR cue Style fe o A Help gt Click Line control to modify the color Line er Cancel thickness and line type 4 Point Sample Intensity ent sec 1000 Raman Shift cm Point Sample Intensity ontisec Points LabSpec5 manual 66 HORIBAJOBIN YVON 8 5 2 Spectra If the active object is a spectral image selecting Graph item of the window context menu opens the spectra Properties dialog which all
6. s Multi Window Min overlap pis SubPixel Use multiveindovu Auto overlap Merge data Adjust intensity Return to first window From Edit here the value of the first limit of the spectral window in the current spectral unit Select the following to activate the associated spectral window q AA Edit here the value of the end limit of the spectral window in the current spectral unit Select the following to activate the associated spectral window Time Edit the relative exposure time in 9o of this window This value is used to compensate the differences of signal level Min overlap pix Define here the overlap between the individual windows in pixels A value of 50 gives generally good results SubPixel Selecting a value gt 1 allows to restore the fine shape features The method is based on the shifting of the spectrograph position on a distance less than the detector pixel size Use multi window Select this box to activate the multi window acquisition mode LabSpec5 manual 24 HORIBAJOBIN YVON Auto overlap Select this box to automatically define the overlapping size Merge data Select this box to create one data object for each active spectral window In the other case a data object is generated for each spectral range corresponding to the spectrograph position Adjust intensity Select this box to adapt the intensity to the same values on
7. 39 O The main icons A A e E o 40 6 1 Data management TOOLS sisas rare EE TEE A E EEA linea 40 JAMES DUI P E 40 CEZ OPEN A PM 40 A K 40 Old PRINT PH EM 40 ESL m i a 8 X 40 LabSpec5 manual 2 HORIBAJOBIN YVON 6 2 Objects display and information coooooouoooo aan aan a eaa a aak enhn nnne nennen nennen nennen nnns 41 02l ANS TO MATIC roda 41 6 2 2 Intensity normalization aida 4 6 2 POIS KS ISTO asa iso la Sm en qeuttedien qn Uds O onu n m NU PRIN dUN 4 A lt D SK 41 6 2 5 lt Dale paramet eaaa tas 41 00 Data Eze ERES ORT LIRE m 42 6 3 1 Spectrum Real Time Display ooonnncnccicicuconooooonononononononnnnononnonnnnnnnnnnnnnnnnnnnnnnnonononnnoninnnnnnnnn 42 6 3 2 Detector image Real Time Display oooooooccnncnonnnnnnnnnnnnnonnnncnnnnnncinanananananinnnanannnnnns 42 6 5 9 Spe Cla accumulation AAA A PRE 42 6 3 4 Spectral Images accumulation ai0sa0ssass0 ettet tror EE maan O vasa Tosa Pee HESSE IEEE PR YER EIE Deed do ENS 42 6 3 5 Spectral Image Propertles smmsmmsssssassstaussvsas ssa m YR HEERS FEES NHE SAT TH Y NRI ve ove do pe ee adesse e aama0n0asama 43 A OT 43 SEE ORO ONERE TOES 43 6 43 Basic data Tea rodri TEE 43 64 1 Baseline COC CH ON ai
8. all the points of the data trace to localize the local maxima Search In the Search Size field define the number of points in the N Levellz n maximum locality The Search Level parameter controls the minimum intensity Sizelpnt i3 value of local maximum A An APPROX function The Peak Approximation procedure can be used to estimate the initial values of the peak parameters The height and width of the peaks are modified by this method the other parameters are not changed The height of the peak is replaced by the intensity value at the peak position point The width is replaced by the difference in position of the peak and the intensity value of the nearest point less then half of height LabSpec5 manual 51 HORIBAJOBIN YVON gt FIT function The Peak Fit algorithm uses the Levenberg Marquart method of non linear peak fitting It is based on the iteratively adjustment of every peak parameter to attempt the minimal value of Y The basic problem of such an approach is that it can be the multitude of possible solutions and depending on the starting values of peak parameters To avoid such a situation you should select the position and shape of the peak as much to presumed solution as possible The Approx procedure allows the initial values to be adapted to the intensity of data points around the peak The other way 1s to fix the parameters which are known You can select the maximum number of iteration to limit the treatment
9. and Close the Laser Position window Laser Position Center x 29 3515 Click on the Cancel button to close the Center Y 19 8529 Help Laser position window without validating the settings Cancel Image Scale The Image scale window allows you to adjust the scale of the video image These parameters are used when using an XY scanning device Stage or Scanner during the acguisition process To access this window run the video preview and then select Scale item in the Acguisition Video menu gt Image scale Scale Y Move stage to Ccanner corner and click button Sieex 201 385 po Sire Y 211765 M Scanner Clear Help Cancel LabSpec5 manual 31 HORIBAJOBIN YVON Corner points Move the XY stage to left up rectangle corner and click on the point 1 Then move stage to right bottom corner and click on the point 2 Program will read the stage position and use this values as rectangle sizes SizeX X size of the rectangle SizeY Y size of the rectangle Stage Click on the button to modify video scale used by XY stage This operation should be done for each microscope objective Scanner Click on the button to modify scanner scale Clear Clears SizeX and SizeY fields for stage Joystick operation LabSpec5 manual 32 HORIBAJOBIN YVON 4 5 Option menu The Option menu commands allows to select the default working unit and to convert the active data ob
10. at maximum level and the one where the spectroscopic signal is at maximum level Scanning Allows you to define which scanning device has to be used to record a spectral image the XY stage or the Scanner Scanning Stage Click on the down arrow icon to di play the X Stage scanning option list Scanner Then select in the list the wished device LabSpec5 manual 24 HORIBAJOBIN YVON Use detector On systems with 2 detectors this option allows you to acquire data either from only the current active detector or from the 2 installed detectors Mee detectar Kus Click on the down arrow icon t display the aa Use detector list Then select the wished option Click on the OK button to validate the settings and to close the window Click on the Help button to retrieve the informations concerning the parameters of this window L TT Lance Click on the Cancel button to close the window without validating the changes setting LabSpec5 manual 25 HORIBAJOBIN YVON 4 4 5 Autosave function The Auto Save window allows to modify the properties of the acguisition auto save option To access this window select Auto Save item in the Acguisition menu Auto save Save acquired data Format LabSpec ngs Folder ic Stemp s d le Year v Month jw Day File Data 1 Hour jv Min iw Count lo Help Cancel
11. ese 66 08 A A TIONIS PO SO In NEIN ud Po ep cnni rupe nN r Ees 66 we EN 1011006 NTT 67 RULES IBS C T m 68 LabSpec5 manual 3 HORIBAJOBIN YVON o OKK IIS IAI SUENENS LabSpec5 manual 4 HORIBAJOBIN YVON 1 LabSpec5 general overview LabSpec 5 is a general data acguisition and data analysis software based on open NexGen module architecture LabSpec 5 controls all the HORIBA Jobin Yvon Raman instruments enables different data acquisition modes eg single spectrum multidimensional data set video 1mage etc The data analysis routines include many commonly used Raman and FTIR tools such as baseline correction linear and non linear filters peaks fitting Kramers Kronig transform etc The mapping technique enables to generate and visualize map by using different spectral features band intensity peak position and so on The module architecture allows to customize software to support specific operation for example read foreign data file format implement data acquisition trigger etc The Visual Basic script language of Microsoft is supported that yield possibility to create macro commands or include specific data analysis function The LabSpec 5 can be used also as Active X control in third part application LabSpec5 manual 5 HORIBAJOBIN YVON 2 LabSpec5 installation LabSpec5 manual HORIBAJOBIN YVON 3 LabSpec5 main window
12. esee iepeptort utri Haut Ite eit ani MENT UT HUY T2NEI SEE SUNIES INE A 202 gi es1 v tsa m s v nn rt v ste 43 5232 DI COC CIO IA A 45 SEEN CEU RET BLA TE TE ETE IRR 46 0 4 4 Fourier anslormation ss asssmsasssate n tsesis va nu bu brc Po E ub eee Eee vM CIVI SR pES eR ouis e e aiaiai 47 6 4 5 Arithmetical operation ssssessessssesesseseeeeeeee eene eene nenne eene e nere ener nne 48 0 4 6 Peaks and Bands Operations a3 3 2222 6pof cioe RUr etm map toim Dieu NUP Net Mint aada essen d arde 49 S ALD ca TOY UL NNI TT TT 55 6 5 Higheyel data Heat asias 56 A A S6 03 2 New speciral pro NG ooo pis 57 os NS A VI lara Ui 58 T The graphics tools Pano RE 59 AER UI DOMO aia 59 TAE E uc PEPEE I IEN AA E O E ETE IE A 59 Ta Multiply data on the CONSOLI 60 TA Add constant to dala AAA A PU EROS 60 To TA S TTO SE 011 DONE io TO ARAE 60 TO Corect data SADO mm X m 60 7 7 lt Linear shift of the scale MiS ronca kannen edia ai anana Te 60 TE 20010 SCANS shea o EE iat nan E E E 60 7 9 Change data intensity Ill iria 60 7 10 Change axes rectangle RTT RETI 60 MM A DE EN EEE EI A E E Rm 60 8 Window context mens idad 6l PP E E A E A A 6l 5 2 lt IN ON AAEE Tt 62 vana UCET RT EE E AE m 62 E Viu T GaGa 63 e 64 8501 S DE A e COEN 64 A A e steeds E E A A EUMD EE UR Ne idub sa E UB OSTIT 65 AP E TET 65 e e ER UE OEI ISIS HIN
13. field you Event Message need to trig manually the data have the possibility readout to customize the Start Click OK to start acquisition a gt C message which will 118 2 This could be just before eac appear data readout or for exemple case of mapping when Hee Comes positionning at a new sample point SEAT y UE Select the corresponding Use box to see the associated message each time the acguisition starts You can customize the message text Sample Select the Use box to see the associated message each time when a new sample point is selected for measurement You can customize the message text LabSpec5 manual 21 HORIBAJOBIN YVON 4 4 3 Real Time Display function The Real Time Display Properties Dialog window allows to modify the data acguisition properties for the Real time CCD image readout Es Detector image RTD function or the spectra readout in adjustment mode ug RTD function Spectrum To access this function select RTD item in the Acquisition menu Properties Image binning 1 Image binning Y 11 Readout zone ET Exposition time sec 11 Help Cancel Image binning X Set the binning factor in the X direction for the CCD image readout This allows to increase the acguisition speed and the signal intensity level Image binning Y Set the binning factor in the Y direction for the CCD image read
14. menu in the Main menu bar 4 1 3 Save function The Save command permits to record the active object in a file for future retrieving To access this command select Save from the File commands menu in the Main menu bar or use the keyboard shortcut Ctrl S or click directly on the following save icon lel in the main icons bar aa ave n 5 single window des Ex Eg M spectrum Silicon 1015 tsf Select here the folder in which you want the file to be recorded List of the files already existing in the chosen folder Click on the Save button to save the selected object file Enter here the name you 3 wish to assign to the file RUE O Click on the Cancel button to abort the Select here the file format Operaren and to close the window LabSpec5 manual 9 HORIBAJOBIN YVON 4 1 4 Save All function The Save All command permits to record all the objects of the active window in a file for future retrieving To access this command select Save All from the File commands menu in the Main menu bar Select here the folder in which you want the file to be Save As recorded Save in eS single window dem E spectrum Silicon 1015 Esf List of the files already Click on the Save existing in the chosen button to save the folder selected object file Enter here the name you wish to assign to the file Click on the Canc
15. print list move the selected parameter to the previous line EA In the print list move the selected parameter to the next line 4 1 9 Exit The Exit command permits to close all the opened objects and guit the application To access this command select Exit from the File commands menu in the Main menu bar or click on the Close icon in the upper right corner of the LabSpec5 main window NGSLabSpec Spectrum s11 DES Close File File Edit Data Acq Service Option Setup Window Help button meh Open Cro Y dh 18 il pt HD ER o d TA cy mre ccs Close Save Ctrl 5 Save All Print Ctrl P Print Preview Print Setup Page 1 spectrum Silicon 1015 Esf 2 KYImage benzen 1103 30 10 Evf HA L 3 Video siMire twf I 4 BOKU schwanninger 3672 tsf Exit Exit e ESO ms HERES mias LabSpec5 manual 16 HORIBAJOBIN YVON 4 2 Edit menu The Edit commands menu enables to exchange data between different applications and facilitates the data analysis process To access the Edit menu click on the Edit button of the main menu bar Undo Chrl 2 CUE Ctrl x Copy Cher DTE FOSLE Format Undo CTRL To undo the last operation Redo CTRL Y To redo the last operation Cut CTRL 4X To cut the active data object in clipboard Copy CTRL X To copy the active data in clip
16. the overlapping parts This adaptation is based on the linear shift of the data Click on the OK button to validate the settings and to close the window Hel N Hep us Click on the Help button to retrieve the informations concerning the parameters of this window L X Lance M Click on the Cancel button to close the window without validating the changes setting LabSpec5 manual 28 HORIBAJOBIN YVON 4 4 7 Detector function The Detector function allows to set the detector parameters These parameters are common for any acquisition process To access to this window select Detector item in the Acquisition menu Detector In this part select the active detector and the i DK readout mode Spectru AN 32 or CCD image PIR Spectrum EI Cancel In the Sizes part enter the readout limits of the detector chip for the mode selected in the Type part 255 0 Iptiaris set here the parameters Highspeed High Gain options attached to the Ln detector ud sl r p Type SENSO Escoria Select the active detector Mode Select the readout mode Single spectrum readout or CCD image mode Sizes SIZE RN ENDE Edit the readout limits of the detector chip To check the readout zone run the detector image Real Time Display by clicking on the icon Options This section groups together parameters specifi
17. time Increasing the number of skip data point allows to check quickly the convergence of the algorithm and the quality of the initial value selection Options Search Edit the Iteration field to modify the maximum iteration Shape BaussLoren ODIO Edit the Skip point field to modify the number of data Search Ft points skipped from the fitting procedure Levell 111 CI Ear The Error box displays the error value as distance between original data and curve sum of all peaks and baselines This value is named usually as x2 Sizelpnt ms Convert Peaks Fitting a Naha Check Baseline box to fit baseline together with the peaks Show Shapes Help P Baseline Close PEAKS function The Peak Table dialog shows the peaks parameters You can edit each of these parameters add new peaks and remove existing ones a Fix Map Fis Fix Map Formula 575855 05 BaussLoren 971647 IT IT 5705 61 F 05 BaussLorenf Lancel 1321 58 7 7 11827 9 7 05 BaussLorenf p a W columns show the values of the peak parameters The number of the parameter depends from the peak shape formula LabSpec5 manual 52 HORIBAJOBIN YVON A peak position cm 1 qe amplitude max Intensity W full width half max cm 1 p gaussian contribution 1 max I TNT CR EON MERE OE integrated area of band I c Check this box to fix the parameter
18. visual zone is large then the data limits One data per view if you check this box the next time data 1s displayed a new window will be created LabSpec5 manual 63 HORIBAJOBIN YVON gt Y scale X scale uses the same scale for both axes useful for 2D image presentation Scale shows the scale properties for each of the window axes From value of low axis limit Orestes value of high axis limit AS es fixes axis limits The scale will be skipped when normalization function is applied Auto enables auto scaling when data object axis 1s changed Prop enables proper scale limits for each data object Log enables logarithm scale for the axis 8 2 Norma 8 3 Colors The Colors Dialog enables the image color properties to be modified Such properties are used when displaying images To access this function click on the right mouse button and select Colors menu items Colors Select here the active color frame W Red iv Green Iv Blue Meg py Adjust Contrast end Brightness A aeee by moving the scale Bar cursors In the Colors frame you can turn on off Red Green or Blue color To make image negative choose Neg check box In the Palette frame select one of the following styles True co All colors are displayed without modification Black and White False color GECI eT Yellow scale Red areas M
19. 1 5 HELP po Open the LabSpec5 Help index LabSpec5 manual 40 HORIBAJOBIN YVON 6 2 Objects display and information 6 2 1 Scale normalization d Automatic scaling optimization of the active data objects 6 2 2 Intensity normalization Normalize the current object in intensity 6 2 3 Pointers centering He own ox co see mm Brings the pointers to the center of the active window 6 2 4 Data sizes This function reports the limits for each dimension of the active object in the following window and allows you to re define it if necessary The Size field reports the number of the spectral points for the correponding axis As Size From To O rit Scale 5 1 cm Extract Es 34272 Cnt E Close Ce Click on the Get button to update the limits of the current active displayed object eee Use the Scale button to apply the limits to the active displayed object Ew Extract the data included in the limits defined in the corresponding fields to build a new object 6 2 5 Data parameters 2 Clicking on this icon displays the array of the acquisition parameters attached to the active object LabSpec5 manual 41 HORIBAJOBIN YVON 6 3 Data acguisition UE The main purpose of this function is to offer a tool to allow you to adjust guickly the focus and the other experimental conditions to maximize the Raman signal Clicking on this icon st
20. 5 1 Laser m Laser Click here to open the list of the installed a laser A Then select in the list the working laser Hele If your instrument is full automated Aramis instrument the set of the necessary optical pieces will be automatically set in place so the chosen excitation light reaches the sample In case this part is not automated on your instrument a warning message similar to the following on will be displayed LabSpec A Please select Laser 632 81 J Cancel When everything is in place click then on the OK button 5 2 Filter Click here to reset the filter wheel and to set in place the filter displayed in the filter field Click here to open the list of the 6 neujsal filters installed Then select the one you wish to be in place Note The reset function of the filter wheel is usefull when for any reason the selected filter is not well in place or does not match the selected one LabSpec5 manual 37 HORIBAJOBIN YVON There are 6 neutral filters installed with the optical densities 0 3 0 6 1 2 3 or 4 no attenuation Po D0 3 Po 2 D0 6 Po 4 D1 Po 10 D2 Po 100 D3 Po 1000 D4 Po 10000 5 3 Confocal hole Initialize the confocal hole by closing the ay K hole and opening it to the previous value MOIE Close the confocal hole JE aperture 3j N Set here the value of the Closed Open the confocal h
21. HORIBAJOBIN YVON LabSpec5 manual HORIBAJOBIN YVON Pea OCC AUS KA MA l 1 STAD SPECS penetal OVE VIC W siii dra 5 PARS Blood ROPE Oe E NSN 6 5 21 ADS 1605 Man Vr UO esencia an eoru Ep edu Ohoroa SEN Nim ied Em kop t rK ohessa o E HUE 7 o A 8 ONES MERERETUR 8 S ELE Io ORI ITO LO LIKI M 9 AL 21056 TURCON ardor 9 A A 9 4 l4 Save AlN COM nia epi incaico 10 A As e E SE PA 10 Z5 c Print Preis 11 Als Print SUD MOCI N iii 12 As e OE E PII PESE 13 EEE ag cscs E vga de 5H A 16 A TG CU css csc css ss ce secre aise A nn E cosepeeeeeeanicues 17 As e A 18 A TUER 19 AA 2 OIS MELON MENU PP TTE 20 AI SI01 TUO ION ansaan se T T E 21 APA 111000 TUTO IO ossaa saa assa a Esan EAE A E STT mm N TEN EN MEUM 21 4 4 3 Real Time Display tuncti0hasssssa de 27 ARA OD ONS TUO DIOE ose nube tr eC EN eR Menu MM EMI MM IE 23 215 AMO Sa TUB CHO e coii et ve osassa Team EO Ute MUR DOMUM DM ENDURO EM 26 AO Mili window DUGCLIORL ena 27 24 1 Detector UA 29 ALS gt AMOO CUS TNC Ugi ai tii 30 A e e ee e LE E TT 31 2 5 5 OPONE ion 33 TO SON TE DIU PETO eben PSN dote So TI AE EE E TT 34 2E ee TASTI S Blonde s Homo Kaa Sa Hao rub USE POI UNE IE vedios mci cbe pM E UUdNddM oU KO M look usa 34 AER TIN 05801015 RU T TU TE 36 516 control pane Pm 37 SEA E o E O EAN A A 37 N TC at ceo E Tm 37 Se LAO dico ea ONG EEE E E E E A 38 A 38 SD DDC OI MM e O E E A 38 AP o ee ee ee ee 39 3 7 Exposure MES i o A 39 3 5 Motorized XX T DIE otto notas
22. New button to use the spectrum as Model Help Allow negative scores Sh If you choose Allow negative scores check box the program Biber RIAA will use any type of combination even if this combination includes negative values Such a combination corresponds to negative concentrations that do not have a physical sense This case often happens when the set of component are not completed or a contribution of some components is near to zero Select Show error map to see the distribution of the error LabSpec5 manual 58 HORIBAJOBIN YVON 7 The graphics tools panel The graphics Tool Panel on the left part of the screen allows you to modify the data appearance and run visual edit operations Following the type of the current data object the tool panel is updated You will find hereafter a description of all the tools available 7 1 Activate the pointer 5 The Pointers permit you to check the coordinates of the data matrix axis at the desired location To activate a pointer click on the pointer icon in the graphics tools bar left bar To change a pointer position click with the left mouse button and drag to the wished position The current pointer coordinates are displayed in the status bar at the bottom of the main window i If the current active data object is a 2D spectral image click on the SpImRed or SplmGreen or N SpImBlue icon to activate the corresponding pointer In any case to initialize the pointer
23. agenta scale AAA puen esents Cyan scale A Na Magenta scale A eenen eane Cyan scale REA erre Y ellow scale Blue ea Green scale A senses ees Green scale Bec ETE Red scale LabSpec5 manual 64 HORIBAJOBIN YVON 8 4 Axes The Axes dialog allows you to customize the appearance of the graph axes DER Spim Pharma Rx E JE Intensity entdsee LEFT Axis I i I 1 i 5 1 MW A vy yv a 1200 Ticks Values E 2 1 QJ BOTTOM Axis Intensity As shown on the picture above there are 4 axis The Axes Dialog allows you to define independently each component of each axis You access to the Axes Dialog by selecting the Axes item of the window context menu Options Options os Ticks Grid Values Labels Tick Check the box to enable the painting of ticks a Dit op Grid Check the box to create a grid for tick levels let M dw Values Check the box to enable the painting of dan 5 number values associated to ticks i NM Intensity D Labels Check the box to create an axis label and n enable customization of the axis label In the Frame 11 Values 12 Times Labels blank field enter the text you wish to be W gt appeared for the corresponding axis ja ces it Sub arid Ticks 30 Look Help Cancel LabSpec5 manual 65 HORIBAJOBIN YVON Style Frame click on the control to modify the color and style of axis edge
24. al data matrices Options Search Shape GaussLoren Ann Search FI Level 111 Sizelpntl 13 Fitting a Wars Iteration 110 Skip pnt jo Shapes Error Help Baseline l Click on button on the right side of the Peaks window to call one of the following functions _Search E Calls the peak auto finder procedure Ap Approximates the height and width of peaks without fitting E Calls the peak fitting procedure Cear n Removes all peaks and baselines of the data Son I Converts the sum shape of peaks and baselines to data trace Pops m Opens tables showing the parameters of peaks vas Opens the tables of variables used in curve formulas Show S Opens the display properties dialog Shapes cias Opens the display properties dialog LabSpec3 manual 49 HORIBAJOBIN YVON Peaks selection The Peak Edit Properties control the automatic and manual peak correction select or edit Peak Shape control to modify the peak curve shape This value will be used when piisi aeh you insert a peak manually or call the peak Search Sh left n procedure wks Aponte Search Edit the Search Min Level box to modify the Levellt fi parameters level for the peak Search algorithm PEN A Edit the Search Size box to modify the number of Sizefpnt I3 points for the peak Search algorithm
25. alled on your instrument it is possible to acquire spectra in function of one or a combination of some specific variable parameters to obtain for example XYZ volume XY mapped images line X Y depth Z time and temperature profiles So the main purpose of this function is to acquire an array 3D for volume 2D for map or 1D for profile of spectra with each spectrum acquired with specific variable parameters like for example position time temperature In all cases the result spectral array 1s saved in one single file for fast and easy data analysis The array below shows some examples of general possible applications following the different additional optional dveices innstalled Main examples of applications Variable parameter Results Optional device used X or Y position Line profile Motorized XY table Scanner Z position Depth or Z profile Motorized Z motor Piezo LabSpec5 manual 42 HORIBAJOBIN YVON Variable parameter Results Optional device used Time Time profile No additional device Temperature Temperature profile Heating cooling stage X and Y position Map Motorized XY table B Motorized XY Z stage or X Y and Z positions Volume l l Motorized XY stage and piezo 6 3 5 Spectral Image properties 6 3 6 Multi window UT Clicking on this icon allows you to define the properties of the spectra acguisition over extended spectral ranges You can also acces to th
26. anual 68 HORIBAJOBIN YVON 8 9 Image3D The Image3D Properties Dialog enables the modification of the appearance of a 3D representation of the data object To open this dialog select the Image3D item from the window context menu Image 3D Type Select between polygon and gradation display mode Style ai E l Type C n g lt p Hel Gradation Select the axis which defines the elp direction of the the color gradation i Gradation jy c Y 2 Cancel gt Click on the Color control to modify the polygon border style and color Color Fill Click on the Fill control to modify the internal polygon fill style 4 Splm Pharma Style Type E qn e lt gt Gradation fj x T YT Zz Color Intensity ontsec Fill Splm Pharma Help Gradation MR IE Cancel Color Fill E Intensity cntsec LabSpec5 manual 69 HORIBAJOBIN YVON 8 10 3DAxes The 3D Axes Dialog allows you to modify the appearance of the axes for 3D representation of data objects You access to this Axes dialog by selecting the Axes item from the window context menu Options First select the axis you wish to customize Options Ticks Check this box to enable the IJ Tiks drawi f ticks rawing oi ticks Iv Values Values Check this box to enable the display of the number values for ticks Label d Label Check this
27. arts in continuous the following cycle Acguisition in single shot window of a spectrum Display of the acguired spectrum Fach spectrum displayed replaces the previously displayed spectrum There is no averaging or spectra accumulation and no extended coverage possible For this use the dedicated accumulation functions described below The exposure time used is the one set in the RTD exposure time field in the control panel Report to the exposure time section in the control panel chapter The CCD pixels read are the one set in the Acguisition RTD function To stop the continuous readout click on the STOP icon located at right end of the main icons bar c 6 3 1 Spectrum Real Time Display E 6 3 2 Detector image Real Time Display E The purpose of this function is the same as the one described in the pevious section applied to the CCD image The CCD image is a 2D image of the intensity of each pixel of the CCD chip Following the parameters set in the Acquisition RTD function this could be the full chip but also a part of the chip it Clicking on this icon allows spectra to be acquired with multiple accumulations and averaging and or with coverage over extended spectral ranges following the parameters setting of the Acquisition Detector function the one of the Acquisition Multi window function 6 3 3 Spectra accumulation 6 3 4 Spectral Images accumulation E With some suitable optional accessories inst
28. ation by a constant value Enter the value in the Const field and click on the Const button to multiply the data object by the constant value Application of a complex mathematical function Enter the mathematical function in the Funcl or Func2 fields and click on the Funcl or Func2 button to apply the correponding function to the data object The mathematical formula can include basic mathematical function negating pow log exp sin COS asin acos atan abs sqrt step For example the sin a formula replaces the data intensities by sinus function The 1000 log abs x 1 converts intensity to logarithm scale The following rules are applied for the variables names the variables a b c correspond to the values of 1 2 data axis the x and y value are consider as intensity of data objects Merge function Click on the MERGE button to multiply data objects into a single LabSpec5 manual 48 HORIBAJOBIN YVON 6 4 6 Peaks and Bands operations Ith gt General presentation The Peak Fitting operation performs a peak search and curve fitting for a single data trace or a multidimensional data set The following list of capabilities is offered by the program Manual or Auto peak selection routine Gaussian Lorentzian Polynomial and other mathematical formulas for peak Fixing peak parameters during curve fitting operation Mapping peak parameters for multidimension
29. board You can select the data format with the Format command Paste CTRL V To insert the clipboard contents You can select the clipboard format with the Format command Format To select the Copy and Paste clipboard data formats LabSpec5 manual 17 HORIBAJOBIN YVON 4 2 1 Format function This command permits to define the format of the data you export to the clipboard or and the one of the data you import from the clipboard To access this command select Format from the Edit commands menu in the Main menu bar gt The Copy Paste data formats selection In the Copy format column select the format of the data exported to the clipboard Copy Paste Properties sa Click on the OK button to validate the settings and Copy format Faste format WE close the window Data Help Tes Data Click on the Cancel button to abort the changes and In the Paste format column select the format Cancel t Picture close the window of the data imported from the clipboard e DataNative NextGen format Can be used to create the exact copy of the data object e TextCreate the text spreadsheet of the active data object e PictureCreate the screen copy picture of the active window LabSpec5 manual 18 HORIBAJOSIN YVON 4 3 Data menu The Data menu commands permit to activate a data object and to see the list of object of t
30. box to create the Mas Center axis output label and edit the label field to customize the Style axis Frame Values 12 Times w X lt gt Y Click on this button to Tile A Labels 12 Times switch between the X and Y axis i Help Cancel Center Click on this button to re i center the 3D object in the active window Style Frame Click on the control to modify the color and style of axis edge Values Click on thes control to modify the font values Ticks Click on the control to modify the color and style of the ticks Labels Click on the control to modify the labels font LabSpec5 manual 70 HORIBAJOBIN YVON 9 Service LabSpec5 manual 7
31. c to the detector model So this section appears differently following the model of the installed detector The most commonly used parameters will be described hereafter It is possible that some parameters does not appear if the correponding option is not available on the on your current detector High speed Check this box to enable high speed data transfer High gain Check this box to enable high gain level of the detector amplifier LabSpec5 manual 29 HORIBAJOBIN YVON OET osas Check this box to subtract the dark current offset from the intensity values Edit the offset value to subtract in the associated field Temperature Click on the amp button to capture the current detector temperature The current temperature read is displayed in this field 4 To set the temperature edit the value to reach i MATE Se in this field and then click on the Set button Check this box to have a continuous refresh of the temperature value 4 4 8 Autofocus function The Autofocus ensures an optimal focus is reached before an acquisition This function allows you to define the properties of the Autofocus function To acces to this window select Autofocus item in the Acquisition menu Autofocus EI Step Enne 1 gt Intensity D Cancel From Set the start position in um for the autofocus movement i sn Set the stop position in um for the autofocus movement Step
32. ctivated gt Check Transparent box to draw a pointer in transparent style In such cases the image of the pointer is superposed with the window background LabSpec5 manual 67 HORIBAJOBIN YVON 8 7 Image The Image Properties Dialog enables to modify the appearance of the images data objects To access this dialog select Image item from the window context menu gt Select Style mode to make or not a linear interpolation of image intensity along X and or Y Options axes Smoothed along column au Help TEE F Cancel Unsmoothed w a L ur Smoothed along p E m hy line Smoothed along diagonal gt Click Color to modify the image color This color will be used when you select overlay mode for the data display 8 8 Scale bar The Scale Bar Properties dialog allows you to modify the display properties of the scale bar and its associated legend of the 2D presentations To access this dialog select Scale Bar item from the window context menu l Scale Bar gt Click on Line control to select the bar border style and color of the scale bar yst E Click on Fill control to select the sacle bar filling style Line Help and color Eil KEN Cancel gt Click on Font control to select they the scale bar legend ea 14 Times v font properties Color Click on Color control to select scale bar legend text color EBR Scale bar legend Scale bar LabSpec3 m
33. display er To close and to remove the current object SAVO ita To save the current object in a file for later retrieval Save A ansias To save all the objects of the current window Data printout management functions Prini m Output to the default printer Print Preview To preview the page before printing Print Setup ees To setup the printer parameters Pag c ana aaal assa To define the layout of the page 1 E Y To close and quit the LabSpec5 application LabSpec5 manual 8 HORIBAJOBIN YVON 4 1 1 Open function The Open command permits to select a previously saved file to load it To access this command select Open from the File commands menu in the Main menu bar or use the my keyboard shortcut Ctrl O or click directly on the following open icon WE in the main icons bar Select here the folder in which the file has been saved Select in the list of files the one you wish to load Click on the Open button to open the selected file Click on the Cancel button to abort the operation and to close the window File name Select here the type of rm Files of type LabSpec tsf buf the researched file 4 1 2 Close function The Close command removes the current object of the active window without saving the changes To access this command select Close from the File commands
34. e limits will be used during the fit You can use number or mathematical formulae in the same way as for Init SHOW function The Peak Fitting Show Properties allows you to customize the display of the fitting results in the data object current window Check Show box to display the following items Click on the Style control to modify and select the style of the selected item Options 3d Style Show o Text shows peak label Hel Arrow shows the peak arrow stick i 16 Times New Ri bm Shape shows the band shape Arrow Mp resi Sum shows the result shapes of the all Shape bands Res shows the residual between the al original data and band sum Residual Format Auto I Check Use data style to control the use data color W Lise data style when drawing the peak tick and label In other cases the Arrow and Text styles will be used Shape multicolor Check Shape multicolor box to personalize the v Attach arrow color for each peak Check Attach arrow box to show the peak label tick immediately above data shape gt SHAPES function The Peak Shapes allows to customize the shape of the peak Edit the shape Name in the left column This name will then appear in the list of the available Formula peak shapes of the Shape item in step Apra SSS Help the Peaks window ti Edit then the corresponding po mcs mathematical Formula in the For
35. e of signal overflow This value appears also in the bottom control panel Data name Set here the data label and the default file name Refresh time This parameter is usefull in case of fast acguisition process or big data sizes The data display is not refreshed on the screen until this time is elapsed Binning factor Define the number of data points per spectrum The signal intensity is proportional to this value but the number of data point and spectral resolution will be reduced in the same way Data mode This parameter is used in case of data accumulation Accumulation number gt 1 a Mi on the down arrow icon to display the Data Data mode p verege I mode list Then select how should be defined the final spectrum LabSpec5 manual 23 HORIBAJOBIN YVON Average Final spectrum Spectrum1 Spectrum2 Spectrumn n Summa Final spectrum Spectrum1 Spectrum2 Spectrumn where n is the value of the accumulation number Spike filter Allows to remove or not the spike cosmic noise of the CCD detector Spike filter Of rd Click on the down arrow icon to display the Spike nra filter option list Double espo Single pass Off No spike removal Double expo The cosmic spikes are removed by comparison of the signal values of the different accu
36. e position of each A O BE Associated tool different predefined component type of OH EEK L3 3 1 1 the to print page didi aaa Text object Associated tool graphic object Associated tool Image object Associated tool Information parameters Bottom of pa Associated tool zz Associated tool The page buttons Load To load an already recorded template Pate To save the current template into a file To validate the settings and to close the Page window EA To close the Page window without validating the current Page settings LabSpec5 manual 13 HORIBAJOBIN YVON Using the Page tools icons To insert a component in the to print page click on the corresponding tool Then move the mouse on the page so a cross appears Then move the cross at the wished location press on the left mouse button and move the mouse to define the size of the corresponding component How to select a component of the to print page Move the mouse on the chosen component and click on the left mouse button Then a dotted rectangle is displayed around the selected component Moving a component of the to print page Select the component Then when the following mouse cursor appears click on left mouse button and drag the mouse Re sizing a component of the to print page First click on the object to select it Selection handles appear around the selected object m of selection Move the mouse p
37. el spectrum Silicon 1015 tsf button to short ihe e as type LabSpec taf operation and to Select here the file format close the window 4 1 5 Print function The Print command prints out to the default printer the objects of the active window The layout page is the one defined with the Page command To access this command select Print from the File commands menu in the Main menu bar or use the keyboard shortcut Ctrl P or click directly on the following print icon in the main icons bar LabSpec Spectrum Chalcopyrite1 Ea File Edit Data Acquisition Options Setup Window Help jeu aM ot to 3 ee tu SIM A gt 4 Morte LS tm S Print icon LabSpec Spectrum Chalcopyrite1 File menu File Edit Data Acquisition Options Setup Window Help e id 48 EI Uf de MIND P 2e ASR Roe as IP te f Close Save Chris E Save Al ptes n BBE Prin EJE En 5 Print Print Preview Print Setup Page 1 Caffeine Sgreen tsf 2 Diamond tsf Exit vi al LabSpec5 manual 10 HORIBAJOBIN YVON 4 1 6 Print Preview function The Print Preview command allows to display the to print page before printing To access this command select Print preview from the File commands menu in the Main
38. ere Al You can edit From and To the corresponding map fields to adjust the limits of Options l Check Base box to subtract From To the spectral regions baseline Hec M 1919462 14734 Green Iw 1297 04 1355 36 Blue iw lv 465 1 46 560 307 generate the map of the 7 Click on the Correct button corresponding ratio m n to modify data on the T selected data point s Check Green blue box to Check Spectrum box to see DK Convert Cancel the spectrum of the selected data point s Click on the OK button to generate the map following the settings and close the mapping window Comet Click on the Convert button to convert the current map into spectral image and close the mapping window Hel Hep NIIN Click on the Help button to retrieve the informations concerning the parameters of this window E T Click on the Cancel button to close the Mapping window without without validating the changes setting LabSpec5 manual 56 HORIBAJOBIN YVON a 6 5 2 New spectral profile The Spectral Profile operation allows you to create and modify the all traces of spectral profile data matrix Spectral profile E Mew Click on the NEW button to take the activated spectrum and create a new spectral profile Edit the Name box to define the spectral profile Add l siete Click on the Add button to take the activated
39. he active window This list is shown as a set of color radio buttons on the right pan of the screen To access the Data menu click on the Data button of the main menu bar sil You can activate one of the data object of the active window by clicking directly in aspi2 the list of the Data menu Datas Select this command to show the full objects list of the active window Datas x LabSpec Spectrum aspi Files Edt Data Acquisition Options Setup Window Help CIS GN LIK S ugE LEN Be 85 ui amp te St Be On the following screen amc i copy the active window contains 2 objects 2 spectra You can activate one object also by clicking on the colored radio button zT Spectrum aspi2 Intensity cnt ABB O Raman Shift fer t i amp Oost SOs Ere agp Tikat poala For Help press F1 LabSpec5 manual 19 HORIBAJOBIN YVON 4 4 Acguisition menu The Acguisition menu commands permit to setup the data acguisition parameters To access the Acguisition menu click on the Acguisition button of the main menu bar Custom Trigger RTD Options Auto save Multi window Detector Autofocus video 4i Custom To customize the data properties IT PNG OOP ostais To set the properties of the manual trigger KID sssusa To set the properties of the Real Time Display acguisition mode OPEN S svevecscvcoees oeeeces To set the
40. image accumulation mode op e Enter here the number of accumulations 5 8 Motorized XY Z Table Position of the different axis Fj Select the small blank box attached to of the motorized XY Z table x do 8 each table moving axis to have the Enter here also the position Y 10 E orresponding position value refreshed in l time even when moving with the ou want the table to be I da 8 a 4 ju JE W joystick LabSpec5 manual 39 HORIBAJOBIN YVON 6 The main icons bar In this bar you will find all the icons correponding to the LabSpec5 main functions for the spectral data acguisition managing and treatment These icons are grouped together by interest center Tools for data management Data object display and information Data acguisition Basic data treatment High level data treatment E Lia EXE T E ITE da GS St OE 9 i te Ee Data management Display and Data acquisition Basic data High level data tools information treatment treatment 6 1 Data management tools 611 CUT Delete the active objects 6 1 2 OPEN 3 m Open a data file 6 13 SAVE E Save the active objects into a file 6 1 4 PRINT Print to the current printer the active to print page For more details please report to the description of the print print preview print setup and page function in the File menu chapter 6
41. ing is based on the direct Fourier data transformation applying filter and apodization function and inverse the Fourier transformation The dialog window shows the real and imaginary Fourier functions and allows you then to select the smoothing property e Fourier Faurier Data r Options Limit 50 Go Apod Na Filter No gt Limit Enter in this field the position of the cutoff point in This parameter defines the smoothing factor 0 full smoothed 100 no smoothing Click on the GO button to apply the value or drag the pointer to select the Limit visually gt Apod Select here the type of the apodization function with apodization value reaching zero at a Limit point NODE e No apodization function Eng Linear function SQM eee eee Parabolic function COS TIYT TYS Cosinus function gt Filter Select here the type of the filter function NOME ee eee eese eoeue No filter function LabSpec5 manual 47 HORIBAJOBIN YVON Traffic Traffic function Po 6 4 5 Arithmetical operation The Math Operation can be used to apply commonly used mathematical formulas to a data object Options Const Const Cons Const Func 1 Func 1 Func 2 Func 2 Addition of a constant value Enter the value in the Const field and click on the Const button to add the constant value to the data object Multiplic
42. is function from the main menu bar Acguisition Multiwindow So please report to this section for detailed explanations 6 3 7 Video a Clicking on this icon starts the continuous video image grabbing To freeze the image press on the STOP icon located at the right end of the main icons bar 6 4 Basic data treatment 6 4 1 Baseline correction Et Clicking on this icon allows you to access the baseline correction functions The Baseline correction performs baseline correction of data by using a manual or automatic approach and converts baseline profile to data array Baseline Options Type Lines lea Degree 2 l anverl Attach IN l Auto Close LabSpec5 manual 43 HORIBAJOSIN YVON Manual Baseline Correction The Manual Baseline Correction method is based on polynomial or line segmented correction using referenced points selected by the user To perform a manual base line correction 1 In the Baseline window options select the baseline Type Line Segmented or Polynomial 2 In the Baseline window options select the Degree for the Polynomial baseline 3 Then in the left object associated graphics tools panel select the Baseline tool JA Moving the mouse inside the window of the active objet moves a cross pointer This enables you to add or move the points by clicking at the desired place By setting the Attach parameter in the options of the Baseline window to Yes the selected base
43. isplayed following the style parameters set in the show function of the Peaks and Bands operations function This tool is also used to define manually peaks for the fitting operation ea o D E gt E E E iu Use this tool to define or modify the height and width of the selected peak for band fitting operation Report to the Peaks and Bands operations chapter for details concerning the use of this tool Use this tool to remove displayed peaks label or to remove bands selected for bands fitting For details concerning this tool report to the Peaks and Bands operations chapter 7 9 Linear shift of the scale limits Use this tool to move the vertical or and horizontal of the active object 7 10 Zoom scale This tool allows you to select the part of the active object you want to zoom Press the left mouse button and draw a rectangle to define the wished part 7 11 Intensity shift t Use this tool to adjust the intensity scale of the active data object 7 12 Axes When clicking on this tool icon a dotted rectangle is displayed To re dimension the rectangle Move the mouse pointer on one of the selection handles until to have the size O D K B E E m pointer lo lt Keep the mouse button pressed and move the selection handle to modify the size of the axes rectangle To move the rectangle Move the mouse pointer anywhere inside the rectangle When the moving pointer e ap
44. ject to the selected units when necessary To access the Option menu click on the Option button of the main menu bar Option s gt ww Lier w Multi nit w Cnb sec Convert Unit Select this command to modify the default spectral and intensity units INE cceceuccoces To give the frequencies in nanometer Wavelength spectral unit 1 cm To give the frequencies in cm 1 Raman shift relative wavenumber spectral unit Citas To give the intensity in Counts Cnt sec To give the intensity in Counts per second Convert To give the active data object in the selected units Multi Select this option to apply any data treatment operation to all the data of the active window LabSpec3 manual 33 HORIBAJOBIN YVON 4 6 Setup menu 4 6 1 Instrument This function allows you to setup the instrument following the needs of the measurement and the configuration of the instrument Actually this function is available only for the Aramis system To access this function select Instrument item in the Setup menu Measure Localization lt Falarizatian r Balm Laser C Ma Vertical E e f Horizontal Honzontal di s C Circular Non Visualization Is i Measure Video On Video Off E Point Mode E TREE AE Tian AUETA e Line Mode Cancel Help The Aramis window is organized by functions Measure localisation Polarization Visualization Measure The software checks
45. line points are attached to the spectrum data Setting this Attach parameter to No allows you to freely place the points anywhere in the window 4 In the Baseline window options use the Style list to select the style to display the baseline calculated from the selected points 5 Click on Sub button to subtract the baseline from the data This operation could be applied to multidimensional data set visualized in one dimensional curve mode m E poi a J vXEC 12 m ut de MI t Yr nr T0 16 S ms amp n Ma a Spectrum d03 Move this cross pointer and click at the wished place to define the baseline points Intensity cnt Options Fo 94 eid SOS Me a A00 i it 5 n i 1T B Lines Clear LabSpec5 manual 44 HORIBAJOBIN YVON Automatic Baseline Correction The Automatic Baseline Correction non peak of the data The algorithm points above the baseline are continues until the fitting errors are number of points is not changed The baseline is then automatically is displayed To calculate the baseline without Baseline Conversion The Baseline Correction operation operation allows the baseline trace to Click on the Convert button to start The baseline is displayed in the selected style iteratively fits a polynomial to the baseline starts with a full set of data points The removed and the fitting procedure less than the predefined va
46. lue or the Click on Auto button to start the procedure subtracted from the data and the finalresult subtraction click on the Fit button replaces the data by the baseline trace This be saved as a data file for future retrieving this function Baseline Clear Click on the Clear button to remove the baseline points chji 6 4 2 Data correction gt The Data correction function allows you to modify all the traces of a multidimensional data matrix Some of these operations require an additional spectrum as a second parameter of the operation Correction Corector Intensity ont Limits From 506 372 Ta 2000 Raman Shift om 1551 65 m dd i LabSpec5 manual 45 HORIBAJOBIN YVON _ Noma TEN The Norma button normalizes all the traces to same area value 100 The Zero button moves all the traces to the minimum intensity level M The Get button takes the activated spectrum and put it in Corrector frame This data object will be used as parameter UO The Del button removes the Corrector spectrum Sub T The Sub button subtracts the Corrector spectrum from all the traces NM The Mul button multiplies all the traces by the Corrector spectrum Com The Corr button subtracts the Corrector spectrum from all the traces The intensity of the Corrector is multiplied to fit the intensity of the trace Limits Select the small blank box if you want tha
47. menu bar gt The Print Preview window The to print page is displayed in the print preview window as it will appeared at print out so you can check the page layout before starting the printing operation To modify the page layout use the Page function of the File menu On top of the print preview window some specific buttons commands are available Print Preview LabSpec Spectrum CYCLO specific commands The to print page HORIBAJCOEIN YVON 708 649 146 il Click on this button or press P key or p key to start the print out Nest Page Click on this button or press N key or n key to display the second page in case of a to print document on 2 pages For the moment the layout of a to print document is only on one page Frey Pace Not available Future expansion AwoPase ix on this button or press T key or t key to display the 2 pages of the document Useful for to print document on 2 pages future expansion Zoomin in Click on this button or press I key or i key to zoom the previewed page LabSpec5 manual 11 HORIBAJOBIN YVON Zoom Dut Click on this button or press O key or o key to zoom back the previewed page This button is available only if the Zoom function has been activated Gose Click on this button or press C key or c key to guit the Print preview function and to return to the LabSpec5 application 4 1 7 Print Setup function The Print Setup c
48. mula column Some of the formula parameters have a predefined meaning a peak amplitude e w peak width p peak position Then use the peak parameters initialization function VAR button dialog to set or modify the initial value of these parameters LabSpec5 manual 54 HORIBAJOBIN YVON 6 4 7 Profile Ej The Profile operation performs a visualization of the vertical or horizontal profile of an image To start a Profile operation Load an image and activate image display window e Select cross line pointer by using window menu Pointer item e Click on the following icon E to open the profile window Profile El E E E Choose Hor or Ver control to select between horizontal and vertical profiles Click on the Corr button to replace the image profile This operation can be useful when you apply some operations to the profile for example smoothing and then you want to set data back to the image LabSpec3 manual 55 HORIBAJOBIN YVON 6 5 High level data treatment i 6 5 1 Mapping E The Mapping procedure generates a map by using the signal intensity in the selected spectral regions Up to 4 maps can be displayed when you load or acguire the spectral image the first three are the average signal intensity and the last is the signal ratio The selection of the spectral region is done with the Red Green or Blue pointer in the spectral image window Check Use box to generate mu
49. mulation So the accumulation number will be increased on the one time in this case Single pass This method allows to remove spike in a single accumulation acquisition by analysing bans for sharpness and intensity This algorithm has to used with care Very sharp Raman bands could be modified Nevertheless this method works fine and save time when looking broad bands as it is the case for Raman analysis of amorphous materials or photoluminescence applications Then select if you wish to remove the eventual spikes or not Autofocus Allows to adapt or not automatically the Z position to obtain a maximal signal level The process is based on the detection of the laser beam reflection Click on the down arrow icon to display the Autofocus options list Autofocus Or wit io pum In the field you can adjust the shift val Bet siti Bee Season Then select if you wish to use or not the Autofocus and at which step of the acquisition process you wish to start the autofocus procedure e AA The autofocus procedure 1s not used in the data acquisition process Before acquisition The autofocus procedure is applied before each data readout Before each point The autofocus procedure is applied at each new measurement point positionning Shift value The shift value allows to adjust the difference between the position where the laser reflection is
50. ng the layout of the to print document with the Page command gt The Parameters window List of the non printed NSParameters tools to manage Print list List of the data parameters the list of the data parameters to data parameters selected to be printed Click on the Style button Parameters to open the NSOptions Enter in the Title window to define the m perla a field the printed E Sample ECL NES design of the array name of the Condition Date Treatment Specto OF uei selected in Filter Laser Click on the OK the print list Spec width Power EE Dbiective iere button to validate the Configuration Accumulation settings and to close Increment Exposition the window Bratina N Enter here the Titles E Click on the Cancel number of columns Col 3 of the printed data ies button to abort the changes and to close the window parameters array gt Example of a printed data parameters array You will find below the data parameters array corresponding to the settings of the parameters screen copy as it appears on the printed document Grating KAT the exposition Remark A column contains 2 fields the data parameter label and the data parameter value gt The Parameters tools Add the selected data parameter to the print list Remove the selected data parameter from the print list LabSpec5 manual 15 HORIBAJOBIN YVON t In the
51. ointer on one of the selection handles until to have the size pointer Or lt Keep the mouse button pressed and move the selection handle and modify the size of the object Modifying or creating the contents of a to print page component Except the data object the contents of all the other objects could be modified by double clicking on the wished component Then a dedicated window appears with the tools to modify the corresponding component The options window for the header page the bottom of page and the Text object The parameters window for the array of experiment information parameters The Open window for the Image object Modifying the general page presentation or display Click anywhere on the to print page on the right button of the mouse to display the following menu Cut Cut To remove the selected component Format Turn Format To define the parameters of the sheet of paper See Page Setup oom Turn To define the page orientation Portrait or Landscape Zoom To define the display of the to print page inside the Page window LabSpec5 manual 14 HORIBAJOBIN YVON gt The Parameters function On the printed document the selected parameters are laid out in an array The Parameters function permits to select the data parameters you wish to be printed and to define the style of the array To access this command double click with the left mouse button on the information parameters object when defini
52. ole to the maximum F value of aperture aperture of the confocal hole 5 4 Slit Initialize the slit by closing the slit and ci VA opening it to the previous value 4 Close the slit aperture m M aged PF Open the slit to the maximum value of Set here the value of the A FRI aperture aperture of the slit 5 5 Spectro Initialize the spectrograph grating position Move to the zero order position Spectro y i Move the spectrograph n and then move to the position value grating to the zero order previously set position 0nm a M po Enter here the spectrograph T 14cm Move the spectrograph grating to the grating position value in highest value of position the active spectral unit LabSpec5 manual 38 HORIBAJOBIN YVON 5 6 Options Click here to select one of the available F spectrograph gratings W 1800 y 100 Select the microscope objective Ke Test d Enter here a name for the next recorded spectral EE data object The software will add an incremental number after the entered name 5 7 Exposure times nter here the exposure time value in seconds to be used for p 5 the spectrum and image RTD acquisition mode rt 5 Enter here the exposure time value to be used for the spectrum and spectral
53. ommand permits to select the printer and to define its properties To access this command select Print Setup from the File commands menu in the Main menu bar gt The Print Setup window Click on this button to check or set the parameters specific Select here the printer to use EDL setup ges to the model of the printer Printer used Report to the manual of your printer for detailed information Hame HP DeskJet 9305 Properties Status Ready Type HP DeskJet 9300 9326 9350 Select here the format of the Where LPTT sheet of paper to use and its nE location in the printer Paper Orientation The orientation parameter is A linked to the one set with the Page function Size Ad C Portrait Source Selection automatique Landsca Click on this button to Network choose a printer on pe your network Click on the OK button Click on the Cancel button to validate the settings to abort the changes and to and to close the window close the window LabSpec5 manual 12 HORIBAJOBIN YVON 4 1 8 Page function The Page command permits to define the layout of the to print document To access this command select Page from the File commands menu in the Main menu bar The Page window Page tools bar Components of the to print page r Header page a n EL Beli Associated toole 1 Fach icon allows to HE A define the size and kikki Sees Data object th
54. out This allows to increase the acquisition speed and the signal intensity level Image zone Define here the chip area you wish to load for the RTD CCD image You can choice to read the full chip matrix select Full or only the chip area corresponding to the spectrum readout area select Spectrum Set here the exposure time value in seconds This value appears also in the bottom control panel Click on the OK button to validate the settings and to close the window Hel Hep PEE Click on the Help button to retrieve the informations concerning the parameters of this window L E Lance mn Click on the Cancel button to close the window without validating the changes setting LabSpec5 manual 22 HORIBAJOBIN YVON 4 4 4 Options function The Acguisition Options window allows to define the data acguisition common properties To access to this window select the Options item in the Acguisition menu Options Exposition Hime sec Accumulation number 1 H Data name Test Cancel Refresh time o Binning Factor i E Data mode Average Spike filter t e Autofocus o D pum Scanning Stam Use detector Active Exposition time Set here the exposure time value in seconds This value appears also in the bottom control panel Accumulation number Set here the number of times you wish to repeat the detector exposure This is useful in cas
55. ows you to modify the display properties of the SpIm object ELI EOR EMI L Spectra Options Line Bl Help Fill 47 Cancel Lines Ma Lr E E lt E E E 1000 Raman Shift cm t gt Click Line control to modify the color thickness and line type Click Fill control to modify the color and fill style of the filled zone Click Lines control to select the representation mode of all the spectra You can select to display All the spectra leave the software to display automatically the necessary spectra so it is readable or as shown on the picture above not to display all the spectra 8 6 Pointers The Pointers dialog allows you to customize the appearance of the pointers You access it by selecting the Pointers item of the window context menu gt Click on the Style control to choose one of the following pointer types Line single vertical line Pointer Cross vertical and horizontal lines DOR Level vertical and horizontal lines Ste The position of horizontal line corresponds to the data Help intensity value Selected r Cancel Double two vertical lines Rect rectangle Deselected Transparent M gt Click on the Active control to choose the line style when the pointer is selected you can drag pointer with the mouse gt Click on the Hidden control to choose the line style when the pointer is dea
56. parameters of the data acguisition such as exposure time binning acguisition mode and so on Auto save To set the Save parameters to save automatically the data at end of acquisition Multi window To set the parameters to define the acquisition spectral regions Detector To set the detector properties such as readout zone gain etc Autofocus To set the autofocus properties VIDEO To set the video acquisition properties LabSpec5 manual 20 HORIBAJOBIN YVON 4 4 1 Custom function The Custom function allows to modify the data properties table during the data acguisition To access this command select Custom in the Acguisition menu e Custom Parameters alue DK Date fev 18 2005 lt Help Operator mite Sample E Cancel Open Dialog when data acquistion started To insert or remove properties click on the right mouse button and select Insert Row or Remove Row menu item Check Open Dialog box to display this dialog automatically when the data acquisition process starts 4 4 2 Trigger function The Trigger function allows you to set and customize the properties of the manual trigger These parameters are common for any acquisition procedure except in RTD mode To access this function select Trigger item in the Acq menu You can select at which level of In the Start or in the the acquisition procedure you Sample
57. pboard This is usefull to retrieve these E values by using Copy Paste functions from other softwares 7 3 Baseline d A 4 Use this tool to define the points to build the baseline to perform a manual baseline correction Moving the mouse inside the window of the active objet moves a cross pointer This enables you to add or move the points by clicking at the desired place JA Use this tool to remove some points of the baseline Move the mouse inside the window of the active object on the point you wish to be removed When the sguare cursor appears click on the left mouse button to remove the wished point 7 4 Manual contant multiplication to data J Use this tool to multiply by a constant the active spectrum This a manual method using the mouse Press the Mul const icon and move the spectrum up or down to the wished position 7 5 Manual constant addition to data Use this tool to add a constant to the active spectrum This a manual method using the mouse Press the Add const icon and move the spectrum up or down to the wished position 7 6 Remove spike Hl Use this tool to remove no desired points like spikes cosmics rays 7 7 Correct data shape This tool allows you to modify a spectrum by correcting each point of the spectrum LabSpec5 manual 60 HORIBAJOBIN YVON 7 8 Peak tools X X This tool allows you to display the wavelength or the wavenumber of the selected peaks The label is d
58. pears press the left mouse button and drag to move the rectangle inside the active window LabSpec5 manual 61 HORIBAJOBIN YVON 7 13 Change 3D axes LabSpec5 manual 62 HORIBAJOBIN YVON 8 Window context menu 8 1 Format You can open Vizualization Format dialog by selecting Format menu item e Format Style Internal Help External Cancel Mode Single Dimension 0 m Use scroll scale Always show scroll bars One data per view Y scale scale Freeze Auto Prop Log a a E I M E gt d E gt Internal Click on this control to modify the color and style of the data field background gt External Click on this control to modify the color and style of the window border Usually it is where the axes are painted Mode Select one of the following data display modes Single only activated data object is displayed Overlay all data objects are displayed on the same place Pile data objects will be shown in the separated rectangles Projection trace of the activated data object will be shown in the separated rectangles Trace single trace of the activated object will be shown Use the right scroll bar to change the trace Dimension 1D 2D 3D gt Use scroll scale check this box to use the window bottom and right scroll bar gt Always show scroll bar check this box to always show the scroll bars when the
59. position move to the window center click on the pointers centering F icon in the main icons bar LabSpec3 offers also the possibility to customize the pointers display by using the pointer function of the Window context menu 7 2 Integral B The Integral Operation can be used to calculate the area of a spectrum peak Click on the Integral icon in the graphics tool panel to open the Integral Operation dialog p Integral Point Sample E fee 3 S ea Vales Apple 300 Fram 1 200 36 a To 135014 Sa Top 2101 64 Bottom 32538 Full 346393 6 Format 6 Digits m Style Help Close T E ER E E E i Line FIII LabSpec5 manual 59 HORIBAJOBIN YVON Ermitas Edit here the the low integral limit You can also drag the pointer to select the limit interactively AA Edit here the high integral limit You can also drag the pointer to select the limit interactively EOD ear Shows the integral result without baseline Doc Shows the baseline value Pull escocia Shows the Top Base value Format Select here the number of digits to display the result numbers In e Click on the control to modify the color and line style i Click on the control to modify the color and style of the background Click on the Apply button to apply the From To Line and Fill settings Co Click on the Copy button to copy the values to the cli
60. s during the fitting operation Map e Check this box to display the map of the parameters This option is available for multidimensional data set Formula Shows the peak shape formula Click on the right mouse button and select Insert row or Remove row menu items to add or remove peaks CLEAR function Remove all peaks and baselines of the data CONVERT function Convert the sum shape of peaks and baselines to data trace VAR function The Peak parameters Initialization dialog enables you to select the initial value for the peak parameters The parameters are displayed in a spreadsheet To move inside the spreadsheet use the arrow keys e Variables Edit Name column to select the peak parameter variable name This name must be the same as in the peak shape formula To edit the name double click on the variable name Edit Init column to select the initial value of the parameters You can use number or mathematical formulae with the the following predefined variables qe c x coordinate of the peak insertion point Nue eee y coordinate of the peak insertion point minx low limit of x data axis A high limit of x data axis miny low limit of y data axis BIDEN E high limit of y data axis UN TIINA KSP maxx minx di oS maxy miny LabSpec5 manual 53 HORIBAJOBIN YVON Edit Min and Max columns to select the parameter limits Thes
61. spectrum and add it to the end of spectral profile Insert Click on the Insert button to take the activated spectrum and insert it to the spectral profile selected by cursor pointer Mela M To removes the trace selected by cursor pointer from the spectral profile m Click on the Help button to retrieve the informations concerning the parameters of this window Close Click on the Cancel button to close the window without validating the changes setting LabSpec5 manual 57 HORIBAJOBIN YVON 6 5 3 Modeling FS Click on Modeling icon to open DCLS Dialog The Direct Classical Least Squares finds the linear combination of data from pure components making up the combination that most closely matches the original data This operation applied to all traces of the original data sets yields the maps of each component The multiplicative constants used on the pure spectra are proportional to the concentrations of respective components The difference between the sample and the linear combination is the residual spectrum which could be useful for diagnostic purposes DCLS requires the spectra of all components presented in the sample and assumes that this spectra are not the changed when the component are mixed together To apply DCLS mapping e Activate the multidimensional Data Matrix Click on the ra icon to open DCLS Dialog e Activate the Spectrum window Properties Enter Model Name Mame Click on
62. t only a part of the trace is used for operation This parameter is used for Norma and Corr operation Limits From 506 37 To 11551 65 W From and To Edit these fields to modify the limits of the selected region Pu 6 4 3 Filtration This function allows linear and non linear smoothing and calculates the derivatives of first and second degrees The Linear Savitsky Golay smoothing and derivative computing are based on the convolution approach which performs a least squares fit of polynomial The larger is the Size value and the lower is the Degree value result in a higher smoothing effect Degree mu l Filtration Set in this field the degree of the polynomial The lower is the degree the more intense is the Smooth smoothing effect Der Size d Derz Set in this field the number of the adjacent data z points to be used for the calculation Median The larger is the Size value the more intense is the Help smoothing effect Close Smooth pele Click on the Smooth button to perform the Savitsky Golay smoothing Click on the Der 1 button to calculate the first degree derivate LabSpec5 manual 46 HORIBAJOBIN YVON De2 M Click on the Der 2 button to calculate the second degree derivate L Click on the Median button to apply the median smoothing The Median smoothing is a non linear data filter method 6 4 4 Fourier transformation The Fourier Smooth
63. the presence of the different motors and their position So if a motor is not present the corresponding commutation appears in gray and could not be selected Measure Localization Retro Select this oprion to set the appropriate mirror to capture the Retro Raman signal Macro 90 Select this option to set the appropiate mirror to capture the raman signal from the macro plate Micro Select this option to set the appropiate mirror to capture the raman signal from the microscope External Select this option to set the appropiate mirror to capture the raman signal from the fiber input Polarization Laser Vertical Setup the 1 2 slide to obtain a vertical polarization of the laser Horizontal Set in place the optical to retrieve the natural polarization of the laser Circular Set in place the 1 4 slide to obtain a circular polarization of the laser LabSpec5 manual 34 HORIBAJOBIN YVON Raman Vertical Set in place the analyser on the raman path to polarize it vertically Horizontal Set in place the analyser on the raman path to polarize it horizontally None Remove the analyser to have no polarization of the raman signal Scrambler option This option is available only if Retro or Macro90 or Micro option is selected Select the scrambler option to cancel any polarization effect on the raman signal Visualization Video on Set in place the camera beamspli
64. tter Video off Remove the camera beamsplitter Trino off Set in place the trino for the selected option Trino on Trino FTIR Measure Point mode Set in place the pinhole and the lenses assembly following the selected measure mode Line mode LabSpec5 manual 35 HORIBAJOBIN YVON 4 7 Window menu The Window menu commands permit to arrange automatically the data windows inside the main LabSpec5 window To access the Window menu click on the Window button of the main menu bar Window Help Cascade Functions of the Tile Window menu gt Arrange Icons 1 Spectrum sil 2 Image siMire List of the different 3 5pIm 5A037260 opened windows P 4 Model lact 5 Map Red w 6 Point Sample 7 Score lact Cascade nasa Cascade the windows so they overlap TTO nai Tile the windows Arrange Icons Arrange the icons at the bottom of a main window LabSpec5 manual 36 HORIBAJOBIN YVON 5 The control panel The Control Panel located on the bottom of the screen contains divisions which are directly related to the System configuration This panel will show only the devices which are installed on the System The description below details all of the available sub units Laser i r Filter pHole 1 Slit p Spectro SUE E n y El ES 4 we eh a TUE Male Po a5 zd x PR m P ym PR vem 9 Tes O Ja BE Sone ll m um Wi MM Hal

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