User Bulletin #66 - Thermo Fisher Scientific
Contents
1. top 10 2 20 5 42 Del S1 Cart bottom 10 2 30 6 41 Del S1 Cart top 10 2 40 7 42 Del S1 Cart bottom 10 2 50 8 41 Del S1 Cart top 10 3 00 9 42 Del S1 Cart bottom 10 3 10 10 41 Del S1 Cart top 60 4 10 11 148 Cartridge Wait 30 4 40 12 41 Del S1 Cart top 60 5 40 13 148 Cartridge Wait 30 6 10 14 42 Del S1 Cart bottom 60 7 10 15 103 Wash Reagent Bock S1 60 8 10 16 104 Wash Solvent Block S1 60 9 10 17 101 Wash Input Block S1 60 10 10 18 102 Wash Output Block S1 60 11 10 19 135 Flush Cart Reagent Block 60 12 10 20 136 Flush Cart Solvent Block 60 13 10 21 137 Flush Input Block 60 14 10 22 138 Flush Output Block 60 15 10 23 131 Dry Cart top 60 16 10 24 259 End 0 16 10 Page 4 of 8 User Bulletin 66 Procise and Procise cLC Protein Sequencers The Sequence After you have created the Cart Wash S1 cycle as above create a method like the one Method shown below Save the method as Cart Wash S1 Note Refer to your instrument user manual for instructions on creating and saving a method Note The cartridge temperature for the wash cycle is higher than the temperature used for normal chemistry PROCISE Column C Cycle Cartridge Cycle Flask Cycle Gradient Delete Row User Bulletin 66 Procise and Procise cLC Protein Sequencers Page 5 of 8 Creating Cartridge Cycle and Wash Method for Procise cLC Systems The Cartridge Cycle If you are using a Pr2. User Bulletin 66 Procise and Procise cLC Protein Sequencers July 23 1999 updated 01 2001 SUBJECT New Cleaning Procedures for Glass Cartridge Blocks Overview The glass cartridge blocks used on the Procise and Procise cLC protein sequencers can retain proteins and or amino acids on the surface or within the channel bored through the blocks This can result in excessive amino acid background This user bulletin describes two new cleaning methods for glass cartridge blocks Aqueous Acetonitrile Cleaning Method The cartridge blocks and lines are washed on the instrument with a 10 or 20 solution of acetonitrile in water Pyrolysis Method In certain cases the aqueous acetonitrile cleaning may not be effective enough on its own It may then be coupled with the pyrolytic cleaning method in which the glass blocks are heated to high temperature in a furnace and any contamination is burned off In This Bulletin This bulletin includes the following topics Topic See Page Safety 2 Cleaning the Glass Cartridge Blocks Creating Cartridge Cycle and Wash Method for Procise Systems Creating Cartridge Cycle and Wash Method for Procise cLC Systems Oo MD AJ wo Pyrolizing the Glass Cartridge Blocks gt Applied KS Biosystems Safety Documentation User Attention Words General Warning User Safety Precautions Ordering MSDSs Page 2 of 8 Five user attention words appear in the t
3. ext of all Applied Biosystems user documentation Each word implies a particular level of observation or action as follows Note This word is used to call attention to information IMPORTANT This word calls attention to information that is necessary for correct operation of the kit or instrument CAUTION This word informs the user that damage to the instrument could occur if the user does not comply with the information It also indicates a potentially hazardous situation that could result in minor or moderate injury to the user WARNING This word informs the user that serious physical injury or illness to the user or other persons could occur if these required precautions are not taken DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury WARNING CHEMICAL HAZARD Some chemicals used with Applied Biosystems instruments or kits may be hazardous and can cause injury illness or death Check regularly for chemical leaks and chemical spills which represent potential safety hazards to the operator If a leak or a spill occurs follow clean up instructions in the Material Safety Data Sheets MSDSs or in the Waste Profile in the Site Preparation and Safety Guide Hazardous Chemical Warnings are prominently displayed on the labels of all hazardous materials Always read the appropriate MSDSs and Waste Profiles before using the chemicals in any way Always wear the appropriate pr
4. is higher than the temperature used for normal chemistry PROCISE Sequence Methods Y MALUNGS Cart Wash Si cLC v Starting Temperatures Cartridge C Flask C Column C Cycles amp Gradients Cycle Cartridge Cycle Flask Cycle Gradient Default Cart Wash 1 cLC Delete Row User Bulletin 66 Procise and Procise cLC Protein Sequencers Page 7 of 8 Pyrolizing the Glass Cartridge Blocks About Pyrolysis In some cases the aqueous acetonitrile wash may not be sufficient to remove all contaminants from the blocks If there is still high background signal after aqgueous acetonitrile cleaning and you have access to a muffle furnace clean the cartridge blocks using the pyrolysis procedure described below Procedure for To pyrolyze the glass cartridge blocks you need Pyrolysis 4 A furnace or oven that can achieve 630 C Shallow pyrex dish es or beaker s Step Action 1 Remove the glass cartridge blocks from the instrument and place them into pyrex dishes Make sure that the sealing surfaces of each block are facing upward Place the pyrex dishes into the furnace and set the temperature to 630 C Make sure the glass cartridge blocks are maintained at 630 C for at least two hours WARNING HOT Allow the furnace to cool down preferably overnight before removing the blocks Note Use new clean disposable powde
5. ocise cLC sequencer create a cartridge cycle like the one shown below Save the cycle as Cart Wash S1 cLC Note Refer to your instrument user manual for instructions on creating and saving a cycle Cart Wash S1 cLC Step Fxn Function Name Time Temp El Time 1 258 Begin 0 00 2 41 Del S1 Cart top 120 2 00 3 42 Del S1 Cart bottom 10 2 10 4 41 Del S1 Cart top 10 2 20 5 42 Del S1 Cart bottom 10 2 30 6 41 Del S1 Cart top 10 2 40 7 42 Del S1 Cart bottom 10 2 50 8 41 Del S1 Cart top 10 3 00 9 42 Del S1 Cart bottom 10 3 10 10 41 Del S1 Cart top 60 4 10 11 148 Cartridge Wait 30 4 40 12 41 Del S1 Cart top 60 5 40 13 148 Cartridge Wait 30 6 10 14 42 Del S1 Cart bottom 60 7 10 15 240 Wash Cart Solvent Block S1 60 8 10 16 241 Wash Cart Reagent Block S1 60 9 10 17 101 Wash Input Block S1 60 10 10 18 102 Wash Output Block S1 60 11 10 19 135 Flush Cart Reagent Block 60 12 10 20 136 Flush Cart Solvent Block 60 13 10 21 137 Flush Input Block 60 14 10 22 138 Flush Output Block 60 15 10 23 131 Dry Cart top 60 16 10 24 259 End 0 16 10 Page 6 of 8 User Bulletin 66 Procise and Procise cLC Protein Sequencers The Sequence After you have created the Cart Wash S1 cLC cycle as above create a method like Method the one shown below Save the method as Cart Wash S1 cLC Note Refer to your instrument user manual for instructions on creating and saving a method Note The cartridge temperature for the wash cycle
6. otective gloves clothing and eyewear when handling chemicals Material Safety Data Sheets MSDSs for hazardous chemicals manufactured by Applied Biosystems will accompany your first shipment To receive additional copies of MSDSs at no extra cost call Applied Biosystems at 800 327 3002 For chemicals required for this instrument but not manufactured or sold by Applied Biosystems please obtain the MSDSs from their manufacturers User Bulletin 66 Procise and Procise cLC Protein Sequencers Cleaning the Glass Cartridge Blocks Advantages of The aqueous acetonitrile cleaning method has a number of significant advantages Aqueous Acetonitrile over the current nitric acid sonication method Cleaning No nitric acid No sonication damage e g chipped or cracked blocks Automated cleaning of blocks on the instrument Cartridge inlet and outlet lines are also cleaned gt gt gt More effective cleaning About the Cleaning The cartridge blocks and lines on the instrument are washed with a 10 or a 20 Solution solution of acetonitrile in water Use the correct cleaning solution for your instrument Instrument Cleaning Solution P N Procise S4B 400314 Procise cLC S4C 402051 WARNING CHEMICAL HAZARD Acetonitrile is a flammable chemical that is irritating to the eyes skin and respiratory system if inhaled It can also cause convulsions or fatal cyanide poisoning Read the MSDS in the Site Prepa
7. r free latex gloves when handling the glass blocks Reinstall the glass cartridge blocks on the instrument with a cartridge seal in place If you have not recently done so carry out the aqueous acetonitrile cleaning as described in Cleaning the Glass Cartridge Blocks on page 3 This is to ensure that the inlet and outlet lines are also clean Copyright 2001 Applied Biosystems For Research Use Only Not for use in diagnostic procedures ABI PRISM and its design Applied Biosystems and Procise are registered trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries ABI is a trademark of Applera Corporation or its subsidiaries in the U S and certain other countries All other trademarks are the sole property of their respective owners P N 4312742B Stock No 119UB01 02
8. ration and Safety Guide Wear appropriate protective eyewear clothing and gloves Procedure for To clean the glass cartridge blocks Cleaning Step Action 1 Create the appropriate cycle and method for your instrument See Creating Cartridge Cycle and Wash Method for Procise Systems on page 4 or Creating Cartridge Cycle and Wash Method for Procise cLC Systems on page 6 Place the S4B or S4C in the S1 bottle position using the Bottle Change Procedure Install glass blocks in holders with only the cartridge seal in place It is not necessary to install a glass fiber filter 4 From the Start Run page set all cartridges to run the Cart Wash S1 or the Cart Wash S1 cLC method for one cycle 5 IMPORTANT After all cartridges have been washed be sure to replace the original solvent heptane on the cLC in the S1 bottle position using the Bottle Change Procedure User Bulletin 66 Procise and Procise cLC Protein Sequencers Page 3 of 8 Creating Cartridge Cycle and Wash Method for Procise Systems The Cartridge Cycle If you are using a Procise sequencer create a cartridge cycle like the one shown below Save the cycle as Cart Wash S1 Note Refer to your instrument user manual for instructions on creating and saving a cycle Cart Wash S1 Step Fxn Function Name Time Temp El Time 1 258 Begin 0 00 2 41 Del S1 Cart top 120 2 00 3 42 Del S1 Cart bottom 10 2 10 4 41 Del S1 Cart
Download Pdf Manuals
Related Search