EpiSonicTM - Epigentek
Contents
1. PAGE 3 PRECAUTIONS The EpiSonic 2000 has been designed constructed and tested to assure your safety as the operator of the sonication device How ever for complete safety and protection of this equipment PLEASE READ ALL OF THE INSTRUCTIONS IN THIS MANUAL CAREFULLY PRIOR TO OPERATION INCLUDING THE FOLLOWING WARNINGS I SAFETY PRECAUTIONS m Do NOT open the sonication generator cover without supervision by an authorized service provider m Do NOT open the chiller unit cover without supervision by an authorized service provider m Do NOT open the ultrasonic converter or high voltage cables without supervision by an authorized service provider m Do NOT operate the sonication generator without any connection or sound processing horn attached to the high voltage cable as this may pose a shock hazard m Do NOT attempt to disconnect the high voltage cable while the sonication generator is running as this may pose a shock hazard m Do NOT plug in the power cord on either side while the sonication generator s power switch is on m Do NOT touch any open cable connections on the sonication generator while the power is on m Do NOT immerse the ultrasonic converter in liquids of any sort or let condensed moisture or liquid drip into the ultrasonic con verter m Do NOT touch the activated sample processing horn or ultrasonic converter as it may cause burns or tissue damage m Do NOT operate the sonication generator while the soundpr2. allows for easy self maintenance Regular maintenance is necessary to facilitate optimal performance it is recommended that you have an EpiSonic service plan It is helpful to periodically inspect the unit both visually and physically Before performing any maintenance procedure make sure the power switch is in the OFF position with the power cord unplugged from the outlet 1 Observe the Power Watts Delivery During your sonication runs if you notice a significant reduction in Watts compared to prior runs then it may suggest there is a loosen ing of the connection between the Ultrasonic Converter and the Sample Processing Horn assuming all other variables and run condi tions are exactly the same In such a case see 2 below 2 Clean and Tighten Connective Areas Sonication devices create intense vibration which can put stress on or loosen the threaded connection between the Ultrasonic Con verter and the Sample Processing Horn over time The Ultrasonic Converter and Sample Processing Horn should be disassembled with the wrench set The threading hole and flat surfaces around this area should be cleaned with a cotton swab dipped in isopropyl alco hol Let the parts air dry and reassemble tightly with the wrench set as shown in Sample Processing Horn Assembly on Page 8 Do not use any lubricants grease or WD 40 on any of the parts unless instructed otherwise For maximum protection this preventive maintenance should be performe
3. CYLCES sections below Note To increase or decrease the amplitude in small increments when the sonication is on depress the AMPL to display Amplitude Setting on the screen then press the A or V key Since the amplitude required is application dependent and subject to the volume and type of sample it may be necessary to adjust the AMPL during sonication 3 SETTING TIME In pulse mode the Time Setting or the processing time is the total time that sonication energy will be delivered to your samples It factors in the Sonication ON cycle times only and disregards the Sonication OFF cycle times This is different from the elapsed time 1 To set the processing time press the TIMER key The screen will display Time Setting Hrs Min Sec 2 Using the numeric keys set the processing time as required For example Time Setting Hrs O Min 10 Sec 00 USA TEL 1 877 374 4368 INT TEL 1 631 755 0888 EPISONIC PAGE 11 3 Press the ENTER REVIEW key The screen will display Time 0 10 00 Pulse Ampl 40 4 SETTING CYCLES In pulse mode or cycled operation sonication will automatically turn on and off depending on your duration of Sonication ON and Sonication OFF settings The ON and OFF pulse duration can be set independently between 1 second and 59 seconds During the OFF portion of the cycle the red indicator on the PULSE key will illuminate 1 To set the cycles press PULS
4. any AC mains voltage throughout the world I REAR PANEL DIAGRAM Xy ENEEER ENENENE mE PEHE E EDENDE DE DE Serial Port HEHE d Cooling Fan HHHH unused AA Expansion Cable E HR EER ge GE ASE GEEEUEEEEE EEUE EE W Er p see N Cable Port ai EA VO PORT d yf O FOOTSWITCH O AN NIA O Poen t watoa E T Fuses Power Cord Port ll SETUP DIAGRAM Sample Processing Horn Line Filter Release Converter Connector ee Soundproof Enclosure gt gt if Sonication ma Power Cord Ez Er lt Chiller Water Release Connector Filter Converter Cable lt Flow Tubing USA TEL 1 877 374 4368 INT TEL 1 631 755 0888 EPISONIC PAGE 7 lil SAMPLE PROCESSING HORN ASSEMBLY Lay Sample Processing Horn upside down on a flat Firmly tighten with Assembly Wrenches Rotate the surface Carefully attach converter by tightening top wrench clockwise and the bottom wrench coun clockwise with hands terclockwise Connect Flow Tubing to the black fittings Connect one end of the Converter Cable to the bottom of the Converter Make sure it is securely attached by turning the fastening mechanism clockwise at the end of the cable IV RECIRCULATING CHILLER ASSEMBLY d Connect the Power Adapter s cord to the back of the Connect the Power Cord to the Power Adapter Plug Recirculating Chiller Power Cord into a grounded power outlet PAGE 8 EPISONIC
5. the Recirculating Chiller once sonication begins For DNA and chromatin shearing during extended use ensure that the water temperature does not exceed 20 C unless specifically instructed in an Epigentek approved protocol for use with EpiSonic 5 Adjust Sonication Timing Because every sample is different along with variations in the sample vessel temperature and water level it is important to determine appropriate pulse on and off program durations and cycles Sonication durations will vary based upon different sample sources and sample concentrations used Every sample is different some are harder to shear or process than others Controlling the pulse on and off durations will also help regulate the rate of which the temperature increases 6 Test Degasification For difficult to process samples degassed water removal of carbon dioxide and or oxygen will allow sonication energy to transmit better You can sonicate the water in the sample processing horn for several minutes to degas the water USA TEL 1 877 374 4368 INT TEL 1 631 755 0888 EPISONIC PAGE 15 7 Maintain Sample Consistency While optimizing your sonication procedure you must maintain the following constant sample conditions a sample volume b sample concentration c sample type or source d cross linking fixation duration for ChIP e concentration of formaldehyde for ChIP use less than 1 and f antibody lot if applicable 8 Monitor and
6. 05 00 USA TEL 1 877 374 4368 INT TEL 1 631 755 0888 EPISONIC PAGE 13 ll HANDLING YOUR SAMPLES PAGE UE TEP S O ES The proper sample vessel is critical to obtaining optimal and reproducible results It is highly recommended to use official EpiSonic branded 0 2ml PCR Tubes Cat No EQC 1000 X02 All sample vessels should be surrounded by water on all sides so there is an even distribution of sonication energy Do not use racks or vessels that will cause the sample to be partially blocked from the water level The bottom of the vessel should touch or be as close as possible to the surface of the sample processing horn for maximum expo sure to ultrasonic energy but avoid direct contact with the sample processing horn s surface unless otherwise instructed Avoid using vessels made of extra thick or hardened plastic The actual sample inside your vessel should be completely submerged just below the water level When determining the volume of water to add remember to take the lengths of the Flow Tubing into consideration Be careful not to cross contaminate wells when handling tube well caps or adhesive covering film Wear gloves Do not allow your sample vessels to move horizontally or vertically during sonication as this may affect consistency of results Sonication energy naturally moves outwards from its origin and there may be a bit of sonication discrepancy along the outer edges of the Sample Processing Horn WWW
7. E key The screen will display Pulse on sec Pulse off sec 2 Using the numeric keys set the ON portion of the cycle then press the ENTER REVIEW key The screen will display Pulse on 10 sec Pulse off sec 3 Using the numeric keys set the OFF portion of the cycle then press the ENTER REVIEW key The screen will display Pulse on 10 sec Pulse off 10 sec 4 Press the ENTER REVIEW key The screen will display Time 0 10 00 Pulse 10 10 Ampl 40 On Cycle Off Cycle PNG 2 EPRISONTCI WWW EPIGENTEK COM INFO EPIGENTEK COM 5 VIEWING OPERATION INFO The REVIEW function provides a window on the process by displaying various operating parameters without process interruption Pressing the ENTER REVIEW key repeatedly during processing will consecutively display the following information a Selected amplitude e g Amplitude 40 b Selected processing time and elapsed processing time e g Set 0 10 00 Time 0 05 00 c Selected pulsing cycle settings in seconds on the left and actual pulsing cycle in seconds on the right 10 10 5 10 Pulse ON Pulse OFF Elapsed ON Elapsed OFF d Amount of power in Watts and accumulated amount of energy in JOULES delivered to the horn e g 20 Watts 0000015 Joules Note The amount of energy displayed will be only for one cycle Initiating a new cycle will reset the display to zero e Elapsed time since processing was initiated e g Elapsed time 00
8. EIS I lic USER MANUAL 2000 SERIES AN ADVANCED HIGH THROUGHPUT SONICATION DEVICE FOR gt DNA SHEARING gt CHROMATIN SHEARING Manufactured in the USA Epigentek Group Inc g a EPI G EN TEK Web www epigentek com E mail info epigentek com Complete Solutions for Epigenetics Tel 1 877 374 4368 USA Tel 1 631 755 0888 Int Fax 1 718 484 3956 PAGE 2 EPISONIC WARRANTY INFORMATION Epigentek warrants that the EPISONIC 2000 sonication generator device is free from defects in material and workmanship under normal use and service for twenty 20 months from the date that the warranty period begins unless otherwise indicated Warranty periods begin on the date of delivery All warranties apply only to the original purchaser of this product Epigentek s obligation under this warranty is limited to the repair or replacement at its sole discretion of any product or part thereof if the product is deemed to have a manufacturing defect upon examination by Epigentek or its authorized repair service provider The sample processing horn and chiller are each covered under a nine 9 month warranty from defects in material and workmanship under normal use and service Included soundproof enclosure box cables tubing and tools are not covered by any warranties Discoloration or paint wear is not covered by any warranties Any warranties shall cover only manufacturing defects and do not cover any damage resulti
9. EPIGENTEK COM INFO EPIGENTEK COM lil OPTIMIZATION TIPS Certain samples will shear or process quite fast and easily Other samples may require higher sonication intensity or a longer processing duration Due to variability between each type of sample you process you will need to optimize the sonication conditions for maximum efficiency You can also refer to Epigentek example protocols provided separately and then modify or fine tune a procedure from there To minimize variability in results use the following recommended steps toward optimization of your conditions 1 Use a Proper Vessel The most suitable sample vessels for optimal transmission of sonication energy are composed of polystyrene or polycarbonate Non EpiSonic branded polypropylene sample vessels are also suitable but since the material can absorb some of the sonication energy before it reaches the sample extended sonication times or higher intensity may be required for certain brands Avoid using brittle plastics which may crack when exposed to long periods of ultrasonic energy For optimal performance always position samples into the central area of the Sonication Processing Horn first then load outwards 2 Maximize Energy Contact For the best transmission of sonication energy into your sample vessel ensure that the bottom of the vessel is close to the surface of the Sample Processing Horn preferably 1mm to 3mm apart The amount of water added should be just above th
10. Record Parameters Make sure to keep a record of your sonication parameters in order to achieve reproducibility or to compare against different param eters Note the following a amplitude setting b sonication ON and OFF times c total sonication duration d water temperature e rate of water circulation if applicable f sample vessel used g conditions of the assay sample itself and h any other parameters that you believe may affect processing results Additional Tips for Chromatin Shearing In addition to the above optimization suggestions the following are also important when working with chromatin e Cell Tissue Fixation Appropriate chromatin cross linking is required Insufficient or over cross linking will cause DNA loss or in creased background Make sure the cross linking time 10 15 min concentration of formaldehyde 1 as final concentration and quench solution 0 125 M glycine are correctly used during the cross linking step of chromatin preparation e Chromatin Quality Make sure that chromatin is properly extracted from a sufficient amount of cells or tissues Chromatin extract should be properly stored at 80 C PAGE I EP SON ies WWW EPIGENTEK COM INFO EPIGENTEK COM POST QPERATION When you are finished processing your samples and do not intend to continue use with the EpiSonic again within a few hours you will need to perform the following steps 1 Power Off All Devices Make sure the so
11. WWW EPIGENTEK COM INFO EPIGENTEK COM USA TEL 1 877 374 4368 INT Z EPIGENTEK Complete Solutions for Epigenetics V MAIN ASSEMBLY stig N i Open the lid of the Soundproof Enclosure and feed the Flow Tubing through the two holes at the bottom of the Soundproof Enclosure Feed the other end of the Con verter Cable through the main opening at the bottom until you can rest the Convert er comfortably and firmly in the opening Connect the white fitting end of other Flow Tubbing to the filter in side of the Filter Connect the Line Filter Release Con nector to the filter out side of the Filter Place the Tube Rack into the Sample Pro cessing Horn so that it rests on the gray in ner rim inside the reservoir Connect the Water Release Connector to the supply connection of the Recirculat ing Chiller Connect the Line Filter Release Connector to the return connection of the Recirculating Chiller Connect the Power Cord to the rear panel of the Sonication Generator Plug the Pow er Cord into a grounded power outlet The device is now installed and ready for use Note Make sure all connections are securely attached TEL oS E75 e oe Connect the white fitting end of one Flow Tubing to the Water Release Con nector l nmu mm ERA ma a mk va 1 ee a rrrrrtrt yt tt EEE EE Ere mi iii EE a 5 n 1 il HEER Connect and faste
12. ate collection and not as unsorted waste For more information consult your institution s disposal guidelines or e mail info epigentek com USA TEL 1 877 374 4368 INT TEL 1 631 755 0888 EPISONIC PAGE 5 COMPONENTS Sonication Generator Soundproof Enclosure Sample Processing Horn Ultrasonic Converter N y D O er Be P NI a a yt Flow Tubing Set Water Release Line Filter Release Filter with Power Cord x2 Connector Connector included Filter Cartridge Recirculating Chiller Converter Cable Power Adapter Tube Rack Assembly Wrench Set PAGE 6 EPISONIC WWW EPIGENTEK COM INFO EPIGENTEK COM INSTALLATION Prior to installing your EpiSonic device please take note of the following m Do not discard the original packaging You may need the packaging materials in the future for servicing your device m The EpiSonic device contains sensitive electronics Always be gentle when removing parts from the packaging box or handling parts in general m To prevent any unnecessary failure in the fuse make sure to always turn off the power supply before connecting or disconnecting the power cord m Confirm that the power is switched to the OFF position m Use only the power cord supplied by Epigentek You can also use an appropriate adapter for your specific country in the event the plug does not match your wall outlet The EpiSonic device uses a universal power supply that recognizes
13. d weekly assuming regular use 3 Clean The Sampling Processing Horn The top surface of the Sample Processing Horn should be gently cleaned with a mild soap solution and wiped down with a wet cloth or towel Do not use any cleaning solvents or abrasives Do not let any water drip into the U trasonic Converter Remember to drain any water after use The Sample Processing Horn is constructed from aluminum and you will notice a bit of white oxidation over time You may also notice black specks in the water occasionally as a side effect of sonication on the rubber rim of the Sample Processing Horn Both of these circumstances are normal and they have no effect on the performance of the Sample Processing Horn 4 Clean The Recirculating Chiller Proper cleaning is important to prevent mold growth in the Recirculating Chiller and Flow Tubing especially if there is a prolonged lack of use You can treat the water used inside the chiller with Clear Bath Spectrum Labs or a similar non reactive product Pump the treated water through a sink or alternate reservoir Solvents may damage the acrylic rim of the Sample Processing Horn 5 Inspect Connections and Cables Inspect the Converter Cable and Power Cord to ensure there is no fraying or damage to the rubber sleeve or insulation Never use a cable with broken end connections exposed wires or frayed insulation 6 Replace Tubings Fittings Release Connectors and Filter Cartridges The Flow Tubings and
14. e sample level so that the sample is completely submerged Avoid overflowing or adding the water past the vessel s height 3 Adjust Amplitude Power The higher the amplitude setting the more energy will be transmitted to your sample and the quicker the sample will be processed However higher amplitude will heat up the water in the sample processing horn more quickly Power measured in wattage is the amount of energy that the sonication generator is delivering to the sonication processing horn not to the sample in order to achieve the amplitude setting Thus even with the same amplitude setting the power output can vary if you change the vessel size type and or composition in addition to the amount of water added During optimization of new conditions of which amplitude is uncertain it is always better to set the amplitude at a lower setting and to adjust upwards 4 Maintain Water Temperature You will want to avoid degradation of your sample or reduction in processing efficiency due to overheating The Recirculating Chiller will automatically provide cold re circulation of water Set the target temperature on the Recirculating Chiller to 2 C Pre cool the water for several minutes before starting sonication When the sonication energy is being generated it will also generate heat which will thus increase the temperature in your water and sample The actual temperature of the water will be a few degrees higher than the target temperature of
15. l support epigentek com Website http www epigentek com WWW EPIGENTEK COM INFO EPIGENTEK COM TABLE OF CONTENTS PrECAUMONS 4245632400054 545004004 EE ET Sne 4 Safety PreCaUUIONS 466 era e keen ee reese dnd ROER FORD EE SE ROSE ees SR ORE N 4 Equipment Protection rs ei eu ewe ea wee ee ee ee coe We wd Rae we ee 4 INTIOGUCHON lt 5 sissies E E E E 5 Background Information 144 4 46 ere ee ves eraan eenaa 5 Technical Specifications EE SS eee eee eee eee eens 5 COMPONEN cassi AE OE eens eee N EE EE POT ees 6 INSCAN AMON ORE NE OE ER bee EE TERE EE Ee 7 Rear Panel Diagram i s os AS oo ERROR Se 6G EE AREA ER ER IR UR ER Rd ARE ES 7 Set DIEET e erer en Geen te ikry sienn be ee EREET EED OOR wee EEEN Gens 7 Sample Processing Horn Assembly 0 0 0 eee ce eee ee eee ee eens 8 Recirculating Chiller Assembly 0 000 c cece eee ee eee eee eee ees 8 MERS eg le OR EE OE OE ER EE eee OR N EE EN Oe 9 ODClANON AAR EE ET EE EE IE RE Es 10 Functions of Keys and Controls ss ss ee ee eee eee eens 10 Handling Your SanDIES a oan evn ER OE SERE REDE ORR vd BO ery ne EER EI EA os 14 Sea ip pas ele Ns AA ORE EO OE EE EE HER OON ONE EET FEE 15 Post Operation sasie sae EER BARS DERS 654 SEE Eee Eee ee ee es 17 Caring For Your DEVICE s 5 423 6050 csnnbeeteetasbae sa DAE DER VASE 18 PCCCSSONGS oi se ses EER SRSA ORDER SR io HE d RE SHE A BE OER SE RS 19 USA TEL 1 877 374 4368 INT TEL 1 631 755 0888 EPISONIC
16. n the other end of the Converter Cable to the rear panel of the Sonication Generator Make sure it is se curely attached by turning the fastening mechanism clockwise at the end of the cable EPISONIC PAGE 9 OPERATION I FUNCTIONS OF KEYS AND CONTROLS LCD Display Key power ON AMPL Key O Key power OFF PULSE Key 0 9 Input Digits TIMER Key Increase Decrease Keys START STOP Key CLEAR Key ENTER REVIEW KEY FRONT PANEL LCD Display Displays prompts and the following control parameters e Amplitude selected e Elapsed time e Output power delivered to the horn in Watts and as e Pulse duration percentage of the total power e Accumulated amount of energy in Joules e Selected duration of processing delivered to the horn e Actual processing time 0 9 Keys Input digits CLEAR Key Clears the preceding entry ENTER REVIEW Key Enters data into the program and selects various parameters for display on the LCD display TIMER Key Used with the numeric keys to set the duration of sonication from 1 second to 9 hours 59 minutes 59 seconds Used with the numeric keys to set the pulse mode The ON cycle and OFF cycle can be set indepen dently from 1 second to 59 seconds Red indicator lights when pulser is in the OFF portion of the cycle Starts or stops the sonication In the STOP mode the red indicator goes off Switches the main power ON Switches the main power OFF AMIPL Controls the amplitude of vibration at
17. nector Line Filter Release Connector Line Filter Converter Cable Assembly Wrench Set Set of 2 Tube Rack USA TEL 1 877 374 4368 INT TEL Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Epigentek Leo Sdi 35 aes Cat EQC 1000 X02 Cat EQC 1000 CCB Cat HEQC 2000 PFC Cat EOC 2000 PSG Cat EOC 2000 PSG 2 Cat EQC 2000 PPH Cat EQC 2000 PSE Cat EOC 2000 PUC Cat EOC 2000 PRC Cat HEQC 2000 PPC Cat EOC 2000 PPCUK Cat EOC 2O000 PPCEU Cat EQC 2000 PFT Cat EOC 2O000 PWER Cat EQC 2000 PFR Cat EQC 2000 PLF Cat EOC 2000 PCC Cat EOC 2000 PSW Cat EQC 2000 PTR EPISONIC PAGE 19 E PI EI EN TEK Copyright 2015 Epigentek Group Inc All Rights Reserved Complete Solutions for Epigenetics EPISONIC is a trademark of Epigentek Group Inc
18. ng from accidents misuse abuse neglect modifications repair or service by any unauthorized parties exposure to extreme temperatures exposure to acids or chemical sol vents failure to comply with the instructions in this user manual or maintenance not in accordance with any information contained in literature accompanying the product Any unauthorized modifications repair or servicing by parties not approved by Epigentek shall void any warranties All returns for repairs or replacement must be accompanied with a Return Merchandise Authorization number RMA which can be obtained through Epigentek s customer service department Equipment should be returned in the original packaging If you no longer have the original packaging you will need to contact Epigentek for approved packaging methods Damage occurred from return ship ping due to poor packing is not covered by this warranty You are responsible for shipping costs when shipping to Epigentek for service Epigentek is responsible for shipping costs when shipping back to you after service WHEN RETURNING ANY MATERIALS TO EPIGENTEK YOU MUST CERTIFY THAT THE MATERIALS BEING RETURNED ARE FREE OF ANY HAZARDOUS INFECTIOUS OR RADIOACTIVE SUBSTANCES AND ALL PARTS HAVE BEEN PROPERLY DECONTAMINATED EPIGENTEK GROUP INC EpiSonic 2000 Made in the United States of America USA Phone 1 877 374 4368 International Phone 1 631 755 0888 Ordering E mail sales epigentek com Support E mai
19. nication program is no longer processing or running PAUSE or STOP You can now safely turn the power switch off at the rear panel of the Sonication Generator Do not leave the EpiSonic on and unattended for extended periods of time as this may damage the LCD display or unnecessarily wear down the internal fan Make sure to also power off the Recirculating Chiller 2 Drain Out the Water Disconnect the Water Release Connector and Line Filter Release Connector from the Recirculating Chiller Lower the tubings on top of a sink or beaker while disconnecting the Water Release Connector and Line Filter from the Flow Tubings to siphon the water out Water will drain out more completely and quickly if it is lower than the surface on which the EpiSonic sits 3 Dry the Sample Processing Horn Remove any vessels and vessel holders from the Sample Processing Horn Using a clean cloth or paper towel thoroughly wipe down any excess water or moisture inside and around the Sample Processing Horn and the Soundproof Enclosure It is normal to see white oxidation on the aluminum surface of the Sample Processing Horn through normal wear and tear but it is not recommended to leave unused water in the Sample Processing Horn for extended periods of time Be careful not to get water or moisture on the ultrasonic converter USA TEL 1 877 374 4368 INT TEL 1 631 755 0888 EPISONIC PAGE 17 PNG T8 EPSON ies CARING FOR YOUR DEVICE The design of EpiSonic
20. oof enclosure box is open or this may result in injury to your ears m Inspect high voltage cable for cracks or rips in the protective outer jacket Do NOT operate the sonication generator with a dam aged cable or this may result in serious injury m Do NOT use any substitute or third party cables only use approved cables specifically designed for use with EpiSonic m Power off and unplug the sonication generator from both sides before attempting to replace any fuses m This device is intended for use in research applications and has not been tested or authorized for diagnostic use m Plug in the power cord directly into the unit e g generator and chiller first before plugging into the outlet in order to avoid ac cidental shock I EQUIPMENT PROTECTION m Do NOT place the EpiSonic device in direct sunlight or near magnets Install the device in an area free from dirt excessive dust and explosive or corrosive fumes Keep the device away from extreme temperature and humidity Do NOT place the generator within a fume hood m Do NOT allow the vibrating horn to touch any hard surfaces or it may overload the sonication generator become damaged or cause damage to the surface m Do NOT turn off the sonication generator while it is in the middle of sonication Press STOP on the generator prior to disabling power m Do NOT let water sit for extended periods of time in the sonication processing horn if unused m Ifthe sample processing ho
21. rn is attached to the converter do NOT hold this assembly by the converter as this may bend or damage the threaded connection m Inthe event of loss of power wait at least three minutes before reapplying power m Make sure your electrical outlet has proper grounding before plugging in the sonication generator m Only use parts provided or approved by Epigentek for use with your EpiSonic device m Install your EpiSonic device according to this manual s instructions Failure to follow the instructions including hand tightening the sample processing horn and converter instead of using the included wrenches may result in damage of the instrument m Do NOT operate the chiller without water m Do NOT store the chiller at temperatures in excess of 60 C Do NOT operate the chiller at ambient temperatures of 40 C or higher m Do NOT ship the chiller with any water remaining inside the pump Always fully pump out the water prior to transport PAGE 4 EPISONIC WWW EPIGENTEK COM INFO EPIGENTEK COM INTRODUCTION Congratulations on obtaining your new EpiSonic 2000 Sonication System from Epigentek You made the right choice by selecting EpiSonic as your primary means for processing your DNA and chromatin samples The sample processing horn is designed for high throughput processing with multiple microtubes but is also suitable for single sample processing if desired The new and innovative design of the double concave sample proce
22. ssing horn allows for the transmission of sonication energy throughout the entire process ing surface leading to a higher consistency and reproducibility in results than other waterbath based methods and b less harsher processing and less over fragmentation than probe based methods I BACKGROUND INFORMATION The technology behind the EpiSonic 2000 is Digitally Adaptive Sonocavitation DAS a principle that is ideal for shearing DNA or chromatin as well as for lysing cells and tissues During the DAS process the electronic signal produced at 20 kHz frequency by a digitally controlled generator is converted into acoustic mechanical energy through a piezoelectric converter transducer When the acoustic energy is produced in water negative pressure is generated and causes the distance between the water molecules to exceed the maximum molecular distance necessary to hold liquid intact Consequently the liquid breaks down to create millions of cavitation bubbles These cavitation bubbles expand in size through several acoustic cycles as more acoustic energy is introduced The size of the cavitation bubbles and the corresponding cavitation energy can be manipulated and digitally controlled by adjusting fully scaled amplitude levels 20 100 in intervals of 1 with a DAS based device such as the EpiSonic 2000 When the acoustic energy is sud denly removed these cavitation bubbles collapse creating intense shock waves in an extremely shor
23. t period of time microseconds which transmit into vessels containing samples This in turn forms highly targeted shearing forces to break up or disperse biomolecular samples including DNA chromatin and tissues in a non contact non invasive manner In a nutshell a DAS based device such as the EpiSonic 2000 is able to shear samples with more precision and reproducibility than non DAS instruments ll TECHNICAL SPECIFICATIONS Input Voltage 100 120 VAC or 220 240 VAC 50 60 Hz Rated Current 10 Amps max slo blo 5 Amps max slo blo Fuse Rating 15 Amps 8 Amps Generator Weight 16 Ibs 7 3 Kg Generator Dimensions 8 W x 15 L x 97H 20 3 cm x 38 1 cm x 22 9 cm Sound Enclosure Weight 24 5 lbs 11 1 kgs Sound Enclosure Dimensions 10 L x 10 W x 16 5 H 25 5 cm x 25 5 cm x 42 cm Chiller Dimensions 7 5 L x 5 W x 7 H 19 cm x 12 7 cm x 17 6 cm Output Voltage 1000 V rms max Output Frequency 20 KHz Operating Environment Temperature 39 95 F 4 35 C Relative Humidity 20 90 Non Condensing For indoor use only Shipping Environment Temperature 35 120 F 2 49 C Relative Humidity 10 90 Non Condensing Ambient Pressure Extremes 40 000 ft 12 000 meters WEEE Directive on Waste Electrical and Electronic Equipment This product contains electrical or electronic materials which must be disposed of properly The end user should dispose this product in an environmentally friendly way through a separ
24. the probe tip Increase Decrease Used with the AMPL key when the unit is on stand by to set the amplitude of vibration at the probe Keys A or V tip Also used to increase or decrease the amplitude in small increments while the unit is running To accomplish this task depress the ENTER REVIEW key twice to display AMPLITUDE CONTROL then depress the A or V key as required PAGE 0 EPSON ies WWW EPIGENTEK COM INFO EPIGENTEK COM 1 POWERING ON The screen will display the power rating and frequency of the EpiSonic 2000 and the following control parameters Time Pulse 2 SETTING INTENSITY Desired amplitude must be set in order for the EpiSonic 2000 to be operational AMPL displays the percentage of amplitude that was previously selected Follow your application protocols for the optimal settings for your application As an example to set the ampli tude at 40 while the sonication is off press the AMPL key and the numeric or A or V keys to achieve a 40 reading on the screen and then press the ENTER REVIEW key To clear an incorrect entry press the CLEAR key The minimum amplitude setting is 20 The screen will display Time Pulse Ampl 40 For continuous operation press the START key to begin the sonication To end the sonication press the STOP key For programmed ON OFF cycle operation known as pulse mode or cycled operation refer to the SETTING TIME and SETTING
25. their paired fittings will build up residue over time resulting in possible clogging or even error messages on the Recirculating Chiller due to change in pump pressure Extensive use of the EpiSonic will increase the rate of such build up It is recom mended to replace the Flow Tubings and Fittings as well as the Water Release Connector Line Filter Release Connector and Filter Cartridge every 3 months Replacement Flow Tubings with Fittings Sets HEQC 2000 PFT Water Release Connector HEQC 2000 PWR Line Filter Release Connector HEQC 2000 PFR and Filter Cartridges EQC 2000 PFC can be purchased directly from Epigentek or can be supplied regularly with a service plan 7 Replace Racks The provided Tube Rack will warp over time especially during extended runs Warping may result in uneven sample processing due to changes in water elevation of your sample vessels Replacement Tube Racks HEQC 2000 PTR can be purchased directly from Epigen tek or can be supplied regularly with a service plan WWW EPIGENTEK COM INFO EPIGENTEK COM ACCESSORIES APPROVED CONSUMABLES 0 2 ml PCR Tube with Strip Caps 1000 box ChIP Buffer Filter Cartridges 4 pack REPLACEMENT PARTS Sonication Generator 100 120V Sonication Generator 220 240V Sample Processing Horn Soundproof Enclosure Ultrasonic Converter Recirculating Chiller Power Cord US Power Cord UK Power Cord Euro Flow Tubing with Fittings Set Set of 2 Water Release Con
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