two chamber place preference data collection
Contents
1. Last Column Element W495 W96 WASZ Wise Widg Profile in Editor CAMED PC R DatasTwo Chamber Place Pref Auto Doors mtp Create Table Create Records Create Freestyle Open nave To edit either the Header Titles or Header Elements click on the appropriate cell in the Edit Table Profiles window Rows and columns can be added to the file First select the desired location then right click to add either the desired row or column Use the right click option titled Paste an Identifier to include subject or session identifying information Note that when using the Paste an Identifier function Header Titles and Header Elements are edited and pasted automatically To save the edited MTP file select Save and create a new filename in the Data subfolder To use this newly edited and saved MTP file verify that the file is selected in the Table Transfer Profile display Figure 6 3 and then click Transfer2. sssssssssseeeeeee enhn hem hhem naeh nna arn nnn nnn 17 SiplslHo ec r LeeuusaNviuuUsaNGEEsseEaEEetenine 18 Beginning amp Running an Experiment sseeeeeeeeeeeee nennen nnns 18 Transtatihg the MED PC Mpe FI euseasasivex suxtbs i xx Rois den n ec i RR on ens Da b ica sees 18 Using the MED PC Load Wizard essossepez sxcuas UYSUCTISTESS NUT VEERIVEUF SSTETE FEM DWaa Rar DK abb WEdE 19 Viewing Changing Variable Values sess nnnm nnnm nennen 24 Mcierecqe m 26 Modifying the MedState Notation Code ceeseee mener 27 Chapter CREDIDI III IL 28 Understanding the Data Files cesses nn nnnm mme nnn 28 Sample Data File ceeesseseeeeeeeeee nennen nmn hne nnne namen enne nane nan 28 Chapter B ooueainivUUNUEENULEMDDEEENFERREREUUHUISESEEEEEXENEXESEEEERERRERTEFEGESNEENANMPNNUENKEUSE 31 Data Analysis Using MED PC to Excel eee enean enarrare arn na 31 Using a Pre Formatted Table Profile MTP file eeeseseeennn n mmn 31 PGi TAS V fll Ccsetescecaveencaumiaesnce E tuentewedaaases niaunceeses Vi UR PER TROP PEPV TRE RID EE 34 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION CHAPTER 1 Getting Started Introduction The purpose of this manual is to give an explanation of the MED State Notation Procedures that comprise the SOF 700RA 25 Two Chamber
3. Activity Any beam break within the current zone Movement A change in the beam broken in the current zone Exploration Breaking of the first beam in a zone Entrance Breaking of any beam beyond the first in a zone Zone Time The amount of time in seconds spent in the zone Figure 1 1 illustrates the two zones as well as the beam numbers and Table 1 1 helps to explain Entrance Exploration and Activity The leftmost column contains all of the beams on the Place Preference chamber The next two columns represent the two zones in the Place Preference chamber Wherever the animal is in the Place Preference Chamber refer to the corresponding column Figure 1 1 Place Prefence Chamber Zones and Beam Numbers Labeled For example if the animal is in the white zone refer to the White column in Table 1 1 The table indicates that any movement within that zone beams Whitel White6 is considered Activity If the animal breaks the first beam in the black zone Black10 it is counted as Exploration If the animal breaks the second beam in the black zone Black11 then it is considered an Entrance Now that the animal has entered the black zone refer to the Black column Table 1 1 Reference Table White1 White2 White3 White4 White5 White6 Activity Activity Activity Activity Activity Activity Entrance Entrance Entrance Entrance Entrance Exploration Black10 Exploration Activity Black11 Entrance Activity Blac
4. M Select All Deselect Al Figure 4 13 Displaying Named Variables from Box 1 Displaying Named Variables from Box 1 Adaption Time min Named Wars Test Time min Vars Bin Size sec Refresh ssue Close Help eeke Display Data from Box Additional Boxes to Update 1 Select All Deselect Al 25 Macros The simplest way to initially create a macro is to record keyboard functions while performing the steps manually Once the commands are in the macro it is easy to create a number of macros with the macro editor The following example illustrates the process of loading Box 1 and changing the Test Time to 30 minutes To begin open MED PC and going directly to the run time screen Close the load wizard if present Before loading or opening the procedure click Macro on the main menu and select Turn On Macro Recorder or click the 8th tool bar item with the cassette tape icon on top A note on the bottom of the display indicates that the recorder is running Open Two Chamber Place Pref Auto Door mpc by clicking Files Open Session Change the variables using any of the methods described above When all settings have been made turn the recorder off again by using the main menu or tool bar Save the macro with a distinctive name The example in Figure 4 14 was named Two Chamber PP 30minTest mac since the Test Time was changed to 30 minutes Figure 4 14 Two Chamber PP 3
5. Display Data from Box display and choose the chamber s to modify By clicking additional boxes in the Additional Boxes to Update section changes made to a single box are automatically loaded to all of the selected boxes Figure 4 11 Changing Variables Screen Displaying Variables from Box 1 E D E Hamed Vars A Array B Array p 000 0 000 o 000 F Gi H I d Marg 0 000 0 000 0 000 5 000 0 000 Refresh E L M MN D 0 000 0 000 0 000 0 000 0 000 F LH R 5 T 0 000 0 000 0 000 0 000 0 000 Issue 0 000 0 000 W Array p 000 0 000 Close n Z 0 000 Help Display Data from Box Additional Boses ta Update C from Box 1 m ra ooo t MES Select All Deselect All The value of any simple variable may be viewed from this screen by clicking an array on the table and each element in that array can be viewed as shown in Figure 4 12 To change a value simply highlight and replace the value in the lower right hand box or use the up down arrows to increment by 1 Click the Issue button for the change to take effect Click Named Variables to produce the display in Figure 4 13 Change variables here as needed MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION Figure 4 12 Displaying Array A from Box 1 Displaying Array A from Box 1 2 Named Wars Vars Refresh lssue Close Help AiD from Bax 1 5 000 A eeek Display Data from Box Additional Boxes to Update
6. 60 35 22 21 0 0 53 09 37 31 22 2 1 29 82 22 15 23 0 60 01 48 39 24 25 White Exploration Counts White Entrance Counts White Zone Time White Activity Counts White Movement Counts 26 12 8 134 57 160 130 27 5 2 25 51 34 21 28 1 1 14 36 18 15 29 1 1 12 12 22 21 Editing the MTP file The MTP file can be edited to customize the transfer process and display the data of most interest See the User s Manual for MPC2XL for explicit instructions about how to modify the MTP file using the Edit Table Profiles screen see Figure 6 6 Header Titles are user defined and can include any information that will help label the data listed below the title Header Elements are the data points that will get transferred from the raw data file into Excel The raw data file will list the elements that can be included in the MTP file e g A Z Figure 6 6 Edit Table Profiles Bl MED PC to Excel m File Help Transfer Data Edit Row Profiles Edit Table Protiles Edit Mode d 3 4 B Header Titles Header Elements subject StartDate IStartTime Experimer Group Header Titles Adaptation Test Time Bin Time Header Elements A A Az Column Titles Black Exe Black Ent Black Zon Black Act Black Mov 1st Column Element B B B2 Bid Bit Increment 5 5 5 5 5 Last Column Element 6 495 6496 Bio Biase B499 Column Titles White Exp White Ent White Zon White Act White Mos 1st Column Element ANTT ANT NEZ AMT VV A Increment 5 5 5 5 5
7. P2 8 will remain on at all times as there are no IR Beams associated with these inputs Figure 2 9 MED Test SuperPort Input Card Screen and DIG 713A Card SuperPort Input DIG f1 2 713 Port Address Offset value P1 Repeat Count P2 Repeat Count ON AT ALL TIMES o U Bb N gt d o 9 e o 2 o 9 o e Q o 2 mE O Oe CHAPTER 3 Software Installation Please refer to the MED PC User s Manual for a complete guide to installing the MED PC software building a valid Hardware configuration with the Hardware Configuration utility and opening and compiling a MSN procedure in the Trans IV utility To install the Two Chamber Place Preference Procedures insert the CD into the CD ROM drive and click Install Two Chamber Place Preference The procedures are copied into the indicated folder Backing Up the Software Med Associates strongly encourages creating backup copies of the programs in case of disk failure Having copies of the original programs may be useful in the future should modifications be made to the existing programs CHAPTER 4 Beginning amp Running an Experiment Translating the MED PC mpc File Programs written in MedState Notation must be translated using Trans IV before they can be executed in this application Open Trans IV icon and select Translation Translate and Compile Select the program s to use for the experiment and click Make Click OK to s
8. Place Preference Data Collection Procedures The files in this package can be found on the disk provided by MED Associates Inc These procedures are intended to be run in MED Associates MED PC software The latest version of MED PC gives researchers the ability to use pre programmed procedures such as these to make hardware control and data collection easy These pre programmed procedures can also be modified to meet the evolving demands of a research protocol Again it is the intent of this manual to explain exactly what these procedures implement and provide guidance into how to interpret what the program code achieves in order to let the user determine how to modify them to match their research protocol demands The manual provides some examples of editing and modifying the procedure s programming code The manual also defines the elements in the raw data file produced by these procedures In addition to this manual refer to the MED PC User s Manual for the installation of the MED Associates interface drivers the MED PC Software and the Delphi Compiler Also refer to the User s Manual for instructions on developing a Hardware Configuration Data file structure file saving format and other related options are also determined by the Hardware Configuration Running the Hardware Configuration software utility that accompanies MED PC sets the Hardware Configuration Its purpose is to assign the inputs and outputs on the interface cards in t
9. step performs the transfer and now the data has been sent to Microsoft Excel Figure 6 4 Specify Data Files to Transfer Specify Data Files to Transfer les 2006 09 01 Ji 12006 09 05 My Recent 12006 03 07 Documents I2006 D9 13 3 Es 2007 07 16 14h22m Subject D dat 8 2007 09 28_15h50m Subject Subject 1 csv Desktop Graphics Subjecti Experiment Graphics Window Subjecti Group SI_Experiment_Subjecti_Groupl Two Chamber Place Pref Auto Doors mtp R Two Chamber Place Pref Man Doors mtp r2008 08 22 dat My Documents My Computer File name 12008 08 22 dat Mu Network Files of type Any File Cancel Pl aces Open as read only 5 Maximize the Microsoft Excel screen and the data should appear in the following format Figure 6 5 Excel Spreadsheet File Edit View Insert Format Tools Data Window Help OSM A SRY BAS o0 amp rf5 z MB 10 Bara 710 BZ Al E Subject B C D 1 Subject StartDate StartTime Experiment Group Box Comment 2 Animal 1 8 22 2008 9 35 33 Experiment 1 Group 1 1 3 4 Adaptation Time Test Time Bin Time S 0 15 60 6 7 Black Exploration Counts Black Entrance Counts Black Zone Time Black Activity Counts Black Movement Counts 8 12 d 755 43 524 541 g 3 3 34 48 52 51 10 2 1 45 64 48 42 11 1 1 47 88 51 48 12 0 0 60 69 63 13 0 0 60 41 35 14 1 1 58 08 37 34 15 0 0 43 71 49 40 16 2 1 49 4 37 34 17 0 0 60 41 40 18 1 1 43 32 34 31 19 0 60 23 16 20 0
10. the following Data files may be opened with note pad word pad or any word processor however be sure they are always saved unformatted in the occasion a data extraction utility such as MPC2XL is used The header information should be self explanatory Data file formats are explained in detail in the MED PC User s Manual Breakdown of Sample File Simple Variables The following simple variables are shown immediately following the header information File C Program Files MED Associates MED PC IV DATA 2008 08 22 Start Date 08 22 08 Date that the program started End Date 08 22 08 Date that the program ended Subject Animal 1 Subject name Experiment Experiment 1 Experiment name Group Group 1 Group name Box 4 Box in MED PC that the program ran Start Time 9235733 Time that the program started End Time 10 43 06 Time that the program ended MSN Two Chamber Place Pref Man Door Name of the program that created this file Ce 1 00 Flag Animal in Maze when C 1 De 0 00 Not Used E 900 00 Elapsed Session Time in Seconds F 60 00 Elapsed Bin Time in Seconds G 2 00 Flag for which side the Animal is starting on H 0 00 Not Used Ls 75 00 Subscript for the Data Time Bins VE 0 00 Not Used K 0 00 Not Used L 0 00 Not Used M 14 00 Movement Flag N 0 00 Not Used O 0 00 Not Used Ps 0 00 Not Used Or 1400 Movement Flag R 0 00 Not Used 81 0 00 Not Used T 0 00 Not Used Us 0 00 Not Used V 0 00 Not Used X 0 00 No
11. 0minTest mac Two Chamber PP 3 minTest MAC Notepad Seles File Edit Format View Help LOAD BOX 1 SUBJ 0 EXPT GROUP PROGRAM TWO CHAMBER PLACE PREF AUTO DOORS A SET Test Time mind VALUE 30 MAINBOX 1 BOXES 1 Once this macro is built use the macro editor to make simple changes such as replacing reward or correct lever values Review the Help file on screen or the MED PC User s Manual for more information on macros and the features offered A START command or message box followed by a START command could be added to the macro it was left off here so changes could be verified before starting the procedure Modifying the MedState Notation Code Permanent changes to the Two Chamber Place Preference procedures can be made to the MedState Notation code To make the same change to the Test Time as shown above do the following Open Trans IV and select File Open to place Two Chamber Place Pref Auto Door mpc into the text editor Scroll down to approximately line 89 note the line counter in the lower right hand corner of the editor to reveal the code shown in Figure 4 15 Figure 4 15 Two Chamber Place Pref Auto Door mpc Line 89 ES TRANS IV C MED PC IVMPCYTwo Chamber Place Pref Auto Doors mpc SEE 18 File Edit Search Options Translation Window Help Bin Size 60 seconda i ECNCKCKCNCHCRCRCRCA RC NCNCNCHCHCACACA ACA RCKCKCKCHKCHChCh Ch ch h h A NK HOA Ch hh A i 2 59 1 X Set Default Values and Seal Data Arrays a
12. A DIG713A DIG713A DIG713A DIG713A DIG713A DIG 713A o MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION Wiring Instructions for Systems with Automatic Guillotine Doors Some of the wiring for the Two Chamber Place Preference system will be completed prior to shipping Complete the remaining wiring by following these steps NOTE Be sure that all hardware is unplugged prior to completing any wiring 1 Using the SG 210CP 2 cable connect the DIG 700F decoder card to the 28 VDC power card as shown below Super Super Super Super Super Super Port Port Port Port Port Port go ceo P e J ce eo amp SG 210CP 2 Cable 2 Using the SG 216B2 cable connect the 28 VDC power card to the DIG 709A 28 VDC power distribution card as shown below Red pin to red connector and black pin to black connector Output Super Super Super Super Super i Port amp Pot Port Port Port Input Input Input Poet 199 1 o pan f e00690090 p TA 000000000000 oo lt 000000000000 ooocQ ooo DIG 700F eu SG 216B2 Cable 3 Each SG 211F cable terminates at one end with a female 25 pin connector The other end terminates with eight tinned wires and two 080 pin connectors This cable is used to control and po
13. C Yertical Columns Table Transfer Profile NO PROFILE amp SSIGMED Transfer Label B Labels Wraneter Choose the MTP file in the Data subfolder that corresponds to the MED State Notation Procedure that was run and click Open For this example Two Chamber Place Pref Auto Door mtp will be used Figure 6 2 Select File to Open Open Two Chamber Place Pref Auto Doors rtp Y Two Chamber Place Pref Man Doors mtp hy Recent Documents Desktop My Documents T hy Computer File name Mu Network Files of type MED FC Table Profile Cancel Places Open as read only Note that MTP file is listed under the Table Transfer Profile Select Labels and Data because selecting these options will print data labels as well as import data Click Transfer Figure 6 3 Transfer Data Bl MED PC to Excel File Help Transfer Data Edit Row Profiles Edit T able Profiles Row Transfer Frafile NO PROFILE ASSIGNED Select Transfer Orientation Column Labels Horizontal Rows iv Data f Mertical Columns Table Transfer Profile CAMED PC P DataxTwa Chamber Place Pref Auto O oors mtp Transfer iv Labels Transfer 43 iw Data NAT rnm V i ILL FN NATA ni LT T T AN E DATA COLLECTION 4 Specify the raw data file to transfer Raw data files are listed by date and then click Open This
14. Chamber Place Preference system will be completed prior to shipping Complete the remaining wiring by following these steps NOTE Be sure that all hardware is unplugged prior to completing any wiring 1 Using the SG 210CP 2 cable connect the DIG 700F decoder card to any 28 VDC power connector on the back of the SG 6080D cabinet as shown below up 2 js s 50 Po EUER Po s e meu SG 6080D ing m H A D H 110 Volts Fuse 8 Amp 250V MINI FB D med aSSociates inc SG 210CP 2 CABLE 2 Using an SG 210TTL 20 cable connect each DIG 713A input card to the corresponding ENV 256C IR controller For example connect the DIG 713A card addressed as 1 to the ENV 256C IR controller associated with Chamber 1 med associates inc ad ENV 256C D Super 16 CHANNEL IR CONTROLLER Port Input P2 P1 1 00 2 00 3 4 eoe 5 00 6 eo 7 eo 8 Receivers Transmitters EHHIHEHI EHEHHI OUTPUT SG 210TTL 20 CABLE 12 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION 3 Using the DIG 700C ribbon cable connect the DIG 700F decoder card to the DIG 704PCI card on the back of the computer Decode gt 20 0 lt m lt nN co lt do DIG 700F 4 Connect the stimulus lights to the Lamp Output connectors on the ENV 226B Connect the light in the white chamber to Channel 1 and th
15. Next button 19 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION The Box Selection screen will appear next as shown in Figure 4 3 From this screen the researcher chooses which boxes will be used in the experiment Select the boxes that will run the experiment by clicking in the radio button next to the box number The figure shows that the Hardware Configuration included only 1 box which was selected Click Next to continue Figure 4 3 The Box Selection Screen The Select a Procedure screen appears next as seen in Figure 4 4 This is where the application to be run is selected The screen displays a list of all the currently compiled procedures Select the desired procedure and then click Next Figure 4 4 The Select a Procedure Screen TWO CHAMBER PLACE PREF AUTO DOORS TWO CHAMBER PLACE PREF MAN DOORS 20 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION The Enter Experiment Data Screen should display next as shown in Figure 4 5 The purpose of this screen is to allow annotations to be added to the data file that is produced by MED PC These annotations will help identify the Subject Experiment and Experiment Group upon which data was collected Comments can be added here as well and the data file can be given a customized file name to help identify it from other data files Enter the information desired and click Next Figure 4 5 Enter Experiment Data Screen Subject
16. R CONTROLLER o Input coc 0 Con eo000000 7 e00000007 Receivers Transmitters OUTPUT ls l 7 SG 2IU0TTL 20 CABLE 8 Using the DIG 700C ribbon cable connect the DIG 700F decoder card to the DIG 704PCI card on the back of the computer Decode e Q 700F MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION 9 Connect the stimulus lights to the Lamp Output connectors on the ENV 226B Connect the light in the white chamber to Channel 1 and the light in the black chamber to Channel 2 as shown below P 10 Using the included SG 216A cables connect the Power Operate connectors on the ENV 226B to the SG 215D3 I O connectors 2 and 3 as shown below Channel 1 Channel 2 Channel 3 10 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION 11 Connect the auto door to the SG 215D3 I O connector 1 as shown below UB BS i E i O72 1 0 3 1 04 eee1 Tove I O 1 mil associates mo SG 215D3 dg 12 Apply power to the SG 6510D Interface Cabinet and the computer The wiring for the system is now complete 11 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION Wiring Instructions for Systems with Manual Guillotine Doors Some of the wiring for the Two
17. SSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION CHAPTER 2 Hardware Hardware Guide The hardware included with the Two Chamber Place Preference system will depend on whether it is equipped with an automatic or manual guillotine door as well as the number of chambers in the system All Systems Figure 2 1 ENV 256C 16 Channel IR Controller med associates inc ENV 256C 16 CHANNEL IR CONTROLLER Receivers Transmitters OUTPUT 2 Figure 2 2 Two Chamber Place Preference Chamber with ENV 256C P i eee i fece Channel 1 Channel 2 Channel 3 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION Figure 2 4 Computer with DIG 704PCI Card T Systems with Automatic Guillotine Doors Figure 2 5 SG 6510D Cabinet with DIG 700F DIG 721 DIG 709A and DIG 713A Cards Output Pur p LJ E o Pott LJ Input F2 P1 1 00 oO o 00990000 9060000007 EY 3 986600003 CO A qe wr a E 0006060005 S6G8G00000 CO Cn pwn 0000600 6080000 e 62 e ceo p amp 49 mBHassucures ma SG 215D3 9 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION Systems with Manual Guillotine Doors Figure 2 7 SG 6080D Cabinet with DIG 700F and DIG 713A Cards e0000000 eooc00000 DIG 713A DIG 713
18. Subject 1 Experiment Experiment 1 Group opt Comments Two_Chamber_PP_Experiment Optional Custom Filename Two_ChamberPP_Subjl_Groupl d oe B re E e The next screen to appear is the Review Choices screen as seen in Figure 4 6 This is a method of confirming that the information received from the Box Procedure Selected is correct If it is not correct select Previous and edit the data If it is correct select Next Figure 4 6 Review Choices Screen Review the data below Box 1 Procedure TWO CHAMBER PLACE PREF AUTO DOOR Subject Subject 1 Experiment Experiment 1 Group Group 1 Comment Two Chamber PP Experiment Filename Two Chamber PP Subjl Groupl Click Next to finish loading the box or Previous to correct errors 21 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION The Alter Session Parameters Screen shown in Figure 4 7 is the next screen to appear and is an important screen for the researcher The Alter Session Parameters screen allows the researcher to alter the parameters by which a procedure executes The Send Start Command Screen appears next The options available on the screen vary depending upon how many boxes are described in the Hardware Configuration Figure 4 7 Alter Session Parameters Screen Adaption Time mir Test Time min Bin Size sec In this example only one box is described in the Hardware Configuration so Figure 4 8 will appear ne
19. TWO CHAMBER PLACE PREFERENCE DATA COLLECTION MED STATE NOTATION PROCEDURE SOF 700RA 25 Manual DOC 215 Rev 1 4 Copyright 2010 All Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com Trademarks MedState Notation MED Associates Inc Registered Trademarks MED PC MED Associates Inc Delphi Borland International Inc MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION TABLE OF CONTENTS Chapter eee 1 STING SLOPE ases mv nd Bep EI RPRN Pari e Ber Weird Eplics n Fn uM De tee Ear M Ped KelUCPEEATE EU Ed 1 OOU CON MT T m 1 Definitions of Data Values crvscsnctavesiinesaternaienaelatasmisieet vetadiengueierdiasaiekaysneeneyaaut 2 Overview OF the PEOCSGQUIFS ausockdexid nuage or IRE da d ek Hoa are br e cte Fact E EEN 3 CHAD CSP 2 m e RI 4 gb e 4 lal Wale lUo T 4 Wiring Instructions for Systems with Automatic Guillotine Doors see 7 Wiring Instructions for Systems with Manual Guillotine Doors esses 12 Hardware Test Proc dure cisissvitaakks terian veia iA 33d oak Gag dE VERI UNE CR da awa VERA add 15 CIUSDEST Sa COP EX EEREEREEVILNERREEERINEEFOE KE ER EE EE nee 17 DOW ANC e E Tr REM 17 TO CALON TEE 17 Backing Up the Software
20. ault 15 minutes and the subsequent elements contain the data for each time bin default 60 seconds B 0 B 1 B 2 B 3 B 4 o Exploration vance Zone Tine Activity Couns Movement 0 12 00 8 00 134 57 160 00 130 00 5 5 00 2 00 25 51 34 00 21 00 10 1 00 1 00 14 36 18 00 15 00 T5 1 00 1 00 12 12 22 00 21 00 20 0 00 0 00 0 00 0 00 0 00 CHAPTER 6 Data Analysis Using MED PC to Excel Using a Pre Formatted Table Profile MTP file MED PC to Excel MPC2XL is a program that helps to import data from MED PC the raw data file format previous section to a spreadsheet program such as Microsoft Excel MPC2XL needs to be installed separately from MED PC Please refer to the User s Manual for MPC2XL for installation instructions Once MPC2XL is installed open the folder that the program was saved to and the MTP files have been saved in a subfolder named Data Follow the step by step instructions below for importing data 1 Open Microsoft Excel and then minimize the window Open MED PC to Excel and the display shown in Figure 6 1 will appear The uppermost file display should be titled Transfer Data Under the Table Transfer window at the bottom of the screen click on Select Figure 6 1 Table Transfer MED PC to Excel a il x Eile Help Transfer Data Edit Row Profiles Edit Table Profiles Row Transte Profile NO PROFILE ASSIGNED Transfer Orientation Column Labels Horizontal Rows TIT TET Data
21. e light in the black chamber to Channel 2 as shown below Repeat this step for each chamber em eee Channel 3 e e 13 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION 5 Using an SG 226B 25 cable connect the Power Operate connectors on the SG 226B associated with Chamber 1 to a 28 VDC power connector on the back of the SG 6080D cabinet SG 6080D 28 VOLTS B f POWER IN H 110 Volts Fuse 8 Amp 250V MINI FB Mis m ed associates int 5G 226B 25 CABLE Lamp Intensity Power em em Channel 1 Channel 2 6 6080D e TERS IN 110 Volts Fuse 8 Amp 250V MINI FB gt D e Med associates inc SG 226B 25 SG 226B 12 op lt gt ee CABLE Channel 1 Channel 2 Channel 3 e Channel 1 Channel 2 Channel 3 e CHAMBER 1 CHAMBER 2 7 Apply power to the SG 6080D Interface Cabinet and the computer The wiring for the system is now complete 14 Hardware Test Procedure Once the wiring is complete and power has been applied to the system the hardware can be tested using the MED Test software application If further information is necessary regarding the use of MED Test please refer to the MED Test User s Manual If the system fails any portion of these tests verify that the system is wired properly and run the test again If t
22. he failure persists it should be reported to MED Associates Technical Support Output Card Lights Auto Door From the MED Test main screen select Standard Card Output Card DIG 720 721 722 and the screen shown below will appear Figure 2 8 MED Test Standard Output Card Screen Standard Output Card DIG 720 7271 7 22 Port Address 780 Offset salue Toggle Bits AutaTest Clear Output Exit If the system is equipped with an Automatic Guillotine Door activating Output 1 should raise the door Activating Output 2 should turn on the stimulus light in the white chamber and Output 3 should turn on the stimulus light in the black chamber When Outputs 2 and 3 are activated adjust the Lamp Intensity knobs on the ENV 226B to verify that the light intensity can be adjusted Be sure to test each Output Card by incrementing the Port Address Input Card IR Beams From the MED Test main screen select SuperPort Input Card DIG 712 713 and the screen shown below will appear Break each of the IR Beams and verify that the corresponding indicator lights up on the MED Test screen When this is complete click Clear Card and allow MED Test to run overnight When testing is complete there should not be any counts recorded on MED Test Be sure to test each Input Card by incrementing the Port Address NOTE The indicator lights on the MED Test screen and the LEDs on the DIG 713A card that correspond with P1 7 8 P2 1 and
23. he interface cabinet for each task controlled by MED PC The particular type of interface card that is supplied in the interface cabinet may vary please refer to the User s Manual provided for instructions on how to configure the type of card that is in the cabinet A valid Hardware Configuration must exist in order for MED PC to interface correctly with the MED Associates Inc hardware This means that one should take the time to create a valid Hardware Configuration before attempting to run the procedures included in this package Should there be any problems the staff at MED Associates Inc is available to answer any questions that may arise Please e mail us at support med associates com with a detailed description of the problem or desired goals so that concise and detailed information may be provided The Two Chamber Place Preference Data Collection procedures are designed to be as easy to use as possible MED Associates Inc understands that researchers do not have the time to devote to programming and hardware design and for that reason we have undertaken that burden for you We sincerely hope that you are satisfied with the products and services we provide and look forward to meeting your future experimental needs as your research program evolves Definitions of Data Values The Two Chamber Place Preference Data Collection procedures generate data files that contain values for Activity Movement Exploration Entrance and Zone Time
24. k12 Entrance Activity Black13 Entrance Activity Black14 Entrance Activity Black15 Entrance Activity Data File Arrays The data file will have arrays B and W which correspond to the Black and White zones Refer to Chapter 5 Understanding the Data Files for a sample data file The first row of each array contains the Totals for the entire session Each row that follows is the data for each time bin which is defined at the beginning of the experiment in the Named Variables Overview of the Procedures If the Two Chamber Place Preference Test chamber is equipped with an auto guillotine door then Two Chamber Place Pref Auto Door mpc should be run Both lights will be on when the procedure is loaded so that the user may make any necessary adjustment or perform testing When the start command is issued all lights are turned off Following the acclimation period the auto guillotine door is opened and the lights turned on At the end of the session the door is closed and lights turned off If the Two Chamber Place Preference Test chamber is equipped with a manual guillotine door then Two Chamber Place Pref Man Door mpc should be run This program uses the computer s speaker to signal the end of the acclimation and test periods The default duration for the audible alert is 5 seconds however the duration of the audible alarm may be modified or the alarm may be turned off using the menu item Shut Off Beep under the Configure menu MED A
25. l 0 001 SET cclimat 0 Al Session Em Li BinSize 60 SET I 5 W I 5 9587 987 BiI 5 387 987 ON WhiteLight BlackLight S2 Change A Session 15 to A Session 30 and save the changes with the same or a new file name such as Two Chamber PP 30minTest mpc Remember if creating a new mpc file name and are using a macro to load boxes the file name in the macro also must be changed Translate and compile the new or changed file as described previously and run MED PC Use the Change Variables screen to view confirm the new values CHAPTER 5 Understanding the Data Files Data can be saved manually by selecting FILE SAVE DATA MANUALLY or FILE SAVE DATA FLUSH The file name that is used to save the data in depends on the option that was chosen in the Hardware Configuration Utility and may also be dependent on the Subject Experiment and Group name provided in the MED PC load wizard Within each data file the headings are created for each Subject Experiment Group Box etc see below Data files may be opened with note pad word pad or any word processor or spreadsheet however be sure they are always saved unformatted in case a data extraction utility such as MED PC to Excel might ever be used Data file formats are explained in detail in the MED PC User s Manual Sample Data File Select Annotated on the file options page during hardware installation to produce a raw data file similar to
26. t Used yd 0 00 Not Used zs 0 00 Not Used Array A Array A contains the control variable values The values are preset to default values in State Set 1 State 1 They are displayed as named variables and can be changed by any of the methods described previously Once a session is started these values should not be changed to insure the integrity of the experiment A 0 A 1 A 2 Row Acclimation Test Time Bin Time Marker Time min min sec 0 Q000 15 00 60 00 Array B Array B contains the data values for the Black Zone The first five elements contain the total counts for the entire test default 15 minutes and the subsequent elements contain the data for each time bin default 60 seconds B 0 B 1 B 2 B 3 B 4 Exploration Entrance n Movement aa o Counts ZONE INS AGE Counts Counts 0 12 00 9 00 765 43 624 00 541 00 5 3 00 3 00 34 48 52 00 51 00 10 2 00 1 00 45 64 48 00 42 00 15 1 00 1 2500 47 88 51 00 48 00 20 0 00 0 00 60 00 69 00 63 00 25 0 00 0 00 60 00 41 00 35 00 30 1 00 1 00 58 08 29 09 34 00 35 0 00 0 00 ATT 49 00 40 00 40 2 00 1 00 49 40 37 00 34 00 45 0 00 0 00 60 00 41 00 40 00 50 100 1 00 3 32 34 00 31 00 55 0 00 0 00 60 00 23 00 16 00 60 0 00 0 00 60 00 35 00 29 00 65 0 00 0 00 53 09 37 00 31 00 70 2 00 1 00 29 82 22 00 15 00 75 0 00 0 00 60 01 48 00 39 00 Array W Array W contains the data values for the White Zone The first five elements contain the total counts for the entire test def
27. tart the translator and it will automatically parse the MedState Notation and then open to a DOS screen to compile the Pascal code Depending on the speed of the computer each of these steps may not be seen If any problems are encountered during this process refer to the on screen help menu or the MED PC Version User s Manual or contact MED Associates Inc for assistance Figure 4 1 Trans IV Control Panel for Translating and Compiling MedState Notation Code i Specify Files to Translate Two Chamber Place Pret Auto Doorz mpc Two Chamber Place Pref Man Doorz mpc MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION Using the MED PC Load Wizard MED PC is designed to help the researcher run an experiment by guiding selection choices through its Experiment Loading Wizard This section will describe how to initiate the Two Chamber Place Pref Auto Door mpc application however the following steps that will also apply to all other mpc procedures Open MED PC and the MED PC Experiment Loading Wizard s Welcome screen shown in Figure 4 2 will appear Figure 4 2 The MED PC Loading Wizard Welcome Screen To avoid this load wizard deselect the checkbox labeled Run this experiment automatically when starting MED PC Close this screen by clicking the Close button Closing this screen immediately reveals the MED PC Run Time Screen shown in Figure 4 9 If the choice to continue with the Loading Wizard is made then click the
28. wer the automatic guillotine door The tinned wires connect to the DIG 721 output cards and the 080 pin connectors connect to the DIG 709A 28 VDC power distribution card The figure and table below illustrate the locations of each of these wires Output 5 00 o 00 Pt O O o9 O99 7 DIG 721 Output o gt Su re Oo o0 O O Gray amp Purple DIG 709A Red scu O O Distribution n DIG 721 DIG 709A C 2 C 2 4 Connect the other end of the SG 211F cable to the male end of and SG 210A cable 5 Connect the female end of the SG 210A cable to the SG 215D3 that corresponds to the DIG 721 card it is connected to For example connect the card addressed as 1 to Chamber 1 C Output O0 O O O OQ g 1 05 1 0 6 1 0 7 1 0 8 ae I il fl of joo o B E 5 Of o ja o s 1 0 1 1 0 2 1 0 3 1 0 4 o oO Medassocates ino SG 215D3 9 Be SG 211F DIG 709A CABLE CO SG 210A CABLE MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION 6 Repeat Steps 2 5 for each additional DIG 721 output card 7 Using an SG 210TTL 20 cable connect each DIG 713A input card to the corresponding ENV 256C IR controller For example connect the DIG 713A card addressed as 1 to the ENV 256C IR controller associated with Chamber 1 MEd associates inc ENV 256C Super 16 CHANNEL I
29. xt If more than one box is in the Hardware Configuration then Figure 4 9 will appear Figure 4 8 Send Start Command Screen for Single Box Configuration 22 MED ASSOCIATES INC TWO CHAMBER PLACE PREFERENCE DATA COLLECTION Figure 4 9 Send Start Command Screen for Multiple Box Configuration In both cases Figure 4 8 and Figure 4 9 the screens are where the researcher decides to either load more boxes send a start signal to boxes that are already loaded or enter the MED PC run time environment without sending a start signal by selecting I am finished with the wizard This option results in the screen shown in Figure 4 10 Figure 4 10 The MED PC Run Time Screen MED PC IV B x File Configure View Macros Help AGO alal B Em i el Box 5 Subject IE E perment IG Group IL Load IP Program Subject 1 Esperment 1 Group 1 11 05 Two Chamber Place Pret Acclimation Time Test Time 15 00 Bin Size 60 00 SHOW S amp 10 SHOWS 11_15 SHOWS 16 20 1 26 2009 11 05 38 Abl File EA Wizard For Loading Boxes 23 Viewing Changing Variable Values Before a start command has been issued any variable may be changed on the MED PC run time screen Simply highlight the value to change and then enter the new value Once a session is in progress change variables by selecting Configure Change Variables or click the 4th tool bar item AX In the lower left hand corner of the Change Variables window find the
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