Ultrospec 2100pro User Manual
Contents
1. Ultrospec 2100 pro User Manual English Deutsch Fran ais Espa ol Italiano RUDE lochrom Biochrom Ltd Certificate No 890333 Declaration of Conformity This is to certify that the Ultrospec 2100 pro UV Visible Spectrophotometer Part number 80 2112 21 22 27 28 Serial number 79000 onwards manufactured by Biochrom Ltd conforms to the requirements of the following Directives 73 23 EEC amp 89 336 EEC Standards to which conformity is declared EN 61 010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use EN 61326 1998 Electrical equipment for measurement control and laboratory use EMC requirements Signed Dated 23 October 2002 David Parr Managing Director Biochrom Ltd Postal address Telephone Telefax Biochrom Ltd 44 1223 423723 44 1223 420164 22 Cambridge Science Park Milton Road e mail enquiries biochrom co uk website http Awww biochrom co uk Cambridge CB4 OFJ England Registered in England No 974213 Registered Office 22 Cambridge Science Park Milton Road Cambridge CB4 4FJ England CONTENTS UNPACKING POSITIONING AND INSTALLATION Essential Safety Notes OPERATION Introduction Keypad and display Basic Modes 1 Absorbance 1 1 Transmission 1 2 Factor Concentration 1 3 Applications 2 Wavescan 2 1 Simple Kinetics 2 2 Reaction Rate 2 3 Standard Curve 2 4 Multiwave and Equation Entry 2 5 Nuc2. u Insert samples as required and press repeat as necessary The assay is shown graphically as it proceeds and reverts to show The result total change in absorbance over the reaction time as defined by the intercepts multiplied by the factor slope and the line quality a coefficient of determination of gt 95 is expected if the assay was carried out over a linear section The slope is always presented as Abs min even in seconds mode Start and final absorbances as well as absorbance difference To see the assay on the whole display press Graph F3 to return press OK F3 Data points can be viewed by pressing Data F1 moving the cursor F2 and F1 To go back and change the parameters press Method F1 Reaction Rate Reaction Rate 0 000 0 004 0 206 Delta A Slope Quality 0 206 0 004 97 3 Method Reference Issue 06 12 2003 Ultrospec 2100 pro English 9 Standard Curve 2 4 The construction of a multi point calibration curve from standards of known concentration in order to quantify unknown samples is a fundamental use of a spectrophotometer a common example is the Bradford determination for proteins This instrument has the advantage of being able to store this curve as a method The procedure to construct the standard curve is as follows Press Standards F3 followed by New F1 and confirm F3 this step is not necessary if this mode is being used for the first time Enter app
3. Introduction Your UV Visible spectrophotometer is a stand alone simple to use instrument with a high resolution liquid crystal display LCD and a comprehensive range of spectrophotometry measurements can be undertaken It works on the basis of light from the xenon lamp being directed by a fixed mirror through the monochromator inlet slit This passes through one of several dependent on wavelength selected filters mounted on filter quadrant the filtered light is then directed onto the holographic grating which produces light of the selected wavelength The light then leaves the monochromator via the exit slit and mirrors focus and direct the light into the sample compartment This passes through your cell containing the sample of interest and then a defocusing lens to a solid state detector unit The resulting signal is then filtered and displayed Your spectrophotometer has the following capabilities e Basic Modes for Absorbance Transmission Factor Concentration e Application Modes for Wavescan Wavelength Scanning Simple Kinetics Reaction Rate Standard Curve Multiple Wavelength Multi Wavelength Equation Entry e Stored parameters for Nucleic Acid quantification and purity checking DNA RNA Oligonucleotide e 18 user defined methods in 3 groups of 6 Methods A B C e Print results from the instrument display in graphical format e Download of results directly to Excel for manipulation and archiving via
4. Issue 06 12 2003 Ultrospec 2100 pro English 17 ACCESSORIES If an accessory is changed press the accessory button on the home page F2 to initialise the instrument in order that the appropriate accessory can be identified Depending on the accessory type a list of options is presented Multiple Cell Holder Accessories e Install by removing accessory in place replacing with the new one turning the central mounting screw until it is finger tight and pressing the accessory button on the home page e All multiple cell holders have the option of being used as a single cell holder This means that there will be no rotation after pressing run 4 position cell changer 80 2106 01 Accommodates cells 10 5Ommm in pathlength 8 position water heated 80 2109 70 cell changer 18 Ultrospec 2100 pro English Requires a water circulating bath Locate round extension of tube restrainer into top of cell changer thumb screw Thread tubes through the tube guide and attach this to the instrument base using the screws provided Replace the front blanking plug on the cell compartment lid with the new one that is provided 6 position Peltier heated 80 2106 04 Requires Temperature Control Unit cell changer 80 2105 49 Insert into socket 3 8 position cell changer 80 2108 01 Spare if required Issue 06 12 2003 Single Cell Holder Accessories e Install by removing accessory in place replacing if necessary the bas
5. composition Extracting nucleic acids from cells is accompanied by protein and extensive purification is required to separate the protein impurity The 260 280 ratio gives an indication of purity it is only this however and not a definitive assessment Pure DNA and RNA preparations have expected ratios of 2 1 8 and 2 2 0 respectively deviations from this indicate the presence of protein impurity in the sample but care must be taken in interpretation of results An elevated absorbance at 230 nm can indicate the presence of impurities as well 230 nm is near the absorbance maximum of peptide bonds and also indicates buffer contamination since Tris EDTA and other buffer salts absorb at this wavelength When measuring RNA samples the 260 230 ratio should be gt 2 0 a ratio lower than this is generally indicative of contamination with guanidinium thiocyanate a reagent commonly used in RNA purification and which absorbs over the 230 260 nm range Background correction at a wavelength totally separate from the nucleic acid and protein peaks at 260 and 280 nm respectively is sometimes used to compensate for the effects of background absorbance The wavelength used is 320 nm and it can allow for the effects of turbidity high absorbance buffer solution and the use of reduced aperture cells The instrument calculates concentration displays 260 280 and 260 230 ratios and compensates for dilution and use of cells that do not have 10mm pathlength
6. appears Press 0 to enter 0 u Press OK F3 to confirm the name entry To enter the equation Abs511 12 5 Abs 720 0 3 100 Use lt to remove any entries still there Press F2 twice to enter Press F1 1 to enter the first absorbance Al wavelength value is defined later Press F1 3 to enter the sign Enter numerical factor 12 5 using the keypad press F3 Press F2 to close the first bracket Press F1 2 to enter the minus sign Press F2 to enter Press F1 2 to enter the second absorbance A2 wavelength value is defined later Press F1 3 to enter the sign Enter numerical factor 0 3 using the keypad press F3 Press F2 twice to close the brackets Press F3 to confirm the equation is correct The two wavelengths for Al and A2 now have to be defined enter 511 and 720 when prompted The dilution factor 100 now has to be entered enter 100 24 Ultrospec 2100 pro English Issue 06 12 2003 Good Laboratory Practice Good laboratory Practice GLP concerns being able to trace experimental results to an instrument an operator and the time the result was obtained so that a laboratory can prove that the instrument was functioning correctly or not Laboratory operator and internal instrument reference names can be entered on the spectrophotometer If the GLP option is on during calibration or re calibration the instrument self checks
7. its integrity for GLP purposes The GLP test of this instrument is essentially a confidence test that it is performing as it was when manufactured and tested For absolute measurements an annual certification service agreement with your supplier is recommended The integrity of the instrument for GLP purposes is quantified from e the calibration status of the instrument the bandwidth this is assessed during calibration by measuring the zero order beam width the wavelength accuracy by comparing to the 881 9 nm xenon emission line the values of built in absorbance filters compared to when the instrument was manufactured or last serviced by an accredited engineer the instrumental stray light The expected values are given in parentheses on the GLP print out after calibration the range of acceptance is defined by the technical specification of the instrument In the unlikely event that the instrument fails calibration or goes out of specification a message will appear on the display In this event the following should be checked is the cell compartment lid closed properly is asample in the light beam if so remove it e is the baseplate plug in place single cell accessory e is the in fill panel at the front of the cell compartment in place Pressing OK after the message GLP Calibration Fail appears confirms that you have accepted the instrument status If you are working in a regulated environment
8. such as a drug discovery laboratory that generates data for GLP GMP activities or reports you should not use the instrument and contact your local service engineer Issue 06 12 2003 Ultrospec 2100 pro English 25 Least squares regression analysis and linearity The slope or best straight line and intercept in a kinetics assay or standard curve determination is calculated from a least squares linear regression of the data The following equations are used where n is the number of data points DD n 2 xy gt x gt x n gt x Intercept gt y gt g slope n Slope Linearity is an estimate of the goodness of fit of the least squares linear regression analysis a perfect fit being 100 It is used in both the Reaction Rate and Standard Curve modes and is expressed by a coefficient of determination r calculated using the following equation oap ee 22 2a SPECIFICATION AND WARRANTY Wavelength range 190 900nm Monochromator 1200 lines mm Aberration corrected concave grating Maximum scanning speed 3000 nm minute Spectral bandwidth lt 3nm Wavelength accuracy Inm Wavelength reproducibility 0 5nm Light source xenon lamp Detectors Photometric range Photometric accuracy Photometric reproducibility two silicon photodiodes 3 000 to 3 000A 9999 to 9999 concentration units 0 1 to 200 T 0 5 or 0 003A to 3 000A at 546 nm whichever is the larger within 0 5 of absorbance v
9. A wavelength scan of a sample can also be obtained for visual inspection of integrity The procedure is as follows for DNA 3 1 RNA 3 2 and oligo 3 3 Enter pathlength of cell 10mm 1 5mm 2 2mm 3 Imm 4 or 0 5mm 5 Select units ug ml 1 ne ul 2 or ug ul 3 e Selectif background correction at 320 nm is required Select if sample scan is required scans 220 to 330 nm with autoscaling e Enter dilution factor Oligo 3 3 only enter conversion factor If not known use 33 e Insert reference and press green run key e This reference scan is used for subsequent samples until changed Insert samples as required and press repeat as necessary 12 Ultrospec 2100 pro English Issue 06 12 2003 e To go back and change the parameters press Method F1 e Press Graph to view the sample spectrum Cell 2 14 27 A Al Concentration ng ul 230 0 173 260 0 098 98 280 0 046 260 230 0 57 260 280 2 14 Method Reference Methods A 4 B 5 and C 6 After defining parameters in any of the applications and prior to measuring a sample a method can be saved To save a method e press stop to return to the home page select one of the three method banks 4 5 or 6 press save F1 and choose an unfilled method by pressing the appropriate number enter the method name see below and press OK F3 A stored method is available as an option directly on the instrument menu To change parame
10. Issue 06 12 2003 Download to Spreadsheet Results can be downloaded directly to Excel when the PC has the Spreadsheet Interface Software installed 80 2110 73 and the two are linked with the serial cable 80 2105 97 detailed instructions are supplied with the software Thus absorbance wavelength data comprising a scan for example can be picked up as columns of numbers and converted to a more conventional graph using the spreadsheet results can then be formatted or manipulated as appropriate prior to inclusion in reports or archiving saving to hard disk Results from all modes of use on the instrument can be output in this way Output is automatic when the key is pressed Messages Most messages are self explanatory and relate to use of the instrument Others relate to the calibration of the instrument on switch on This instrument has One or more of the parameters tested for during GLP failed 1 or more GLP calibration is out of specification see Appendix You can tests accept this status and continue to use the instrument as normal but you may to contact your local service engineer Failed to find Abs Failed to calibrate properly contact local service engineer Failed to find Ref 1 Failed to calibrate properly contact local service engineer Failed to align filters Failed to calibrate properly contact local service engineer Failed to align grating Failed to calibrate properly contact local service engineer
11. a serial interface lead to a PC e Self test diagnostics for GLP purposes A range of accessories further enhances the capability of the instrument The home page provides access to user modes system utilities and accessory identification and set up 4 Ultrospec 2100 pro English Issue 06 12 2003 Keypad and display Ultraspec 2100 pro Uvivisible Spectrophotometer B Basic Modes B Applications B Nucleic Acids GB Methods A B Methods B G Methods C mur a ra Press the soft key on the keypad directly below the corresponding option on the display F1 F2 and F3 to select that option For example on the home page above e press F1 to take you to System Utilities e press F2 to identify the type of cell changer holder that has been fitted e press F3 to toggle the display back light on off display contrast can be changed within System F1 Press to print result if auto print is off or to re print result if auto print is on to back space in order to correct text and characters in appropriate boxes to start making measurements and print results green run key Oot to stop making measurements or entering parameters and return to the home page use as an escape mechanism red stop key Press the corresponding number on the keypad to enter the user mode choices for example 1 followed by 1 is Absorbance mode whereas 2 followed by 4 is Standard Curve Mode Issue 06 12 2003 Ultrospec 2100 pr
12. above The GLP calibration details can be printed out for record purposes by pressing F2 if required note they are printed automatically depending on the specified GLP calibration interval see below Set up To adjust the contrast of the display to suit lighting conditions press Contrast Y or Contrast to decrease or increase F1 or F2 respectively Clock 1 Press OK F3 to cycle through year month day hour minute and use F1 or F2 to adjust the parameter down or up as appropriate Customise 2 Instrument description for example asset number operator name and replacement group names for Methods A B and C for example application types or operator name if a multi user environment can be entered here To enter a name press appropriate key on keypad to cycle through options of lower case letter numbers and upper case letters for example pressing key cycles through abc2 ABC Preferences 3 Set your preferences as follows u Sample number prompt no yes enables entry of sample number between 1 999 prior to running an experiment rather than starting from Sample 1 again Autoprint on off if off results can be printed manually using key e Printer Default graph scale 0 3 0 2 0 1 0 0 5 and Autoscale e Confirm exit from application no yes Key click on off GLP 4 Refer to Appendix for more information This option determines whether GLP is on or off in terms of printing
13. alue to 3 000A at 546 nm Stability Stray light Digital output Sample compartment size Dimensions 0 001A per hour at 340nm at 0A lt 0 05 T at 220nm using Nal and lt 0 05 T at 340nm using NaNO2 9 pin serial and Centronics parallel 210 x 140 x 80mm 510 x 350 x 160mm Weight Power requirements 13kg 100 240V AC 10 50 60Hz 80 VA Safety Standard EN61010 1 EMC emissions EN 61326 2 3 Generic emissions EMC immunity EN 61000 4 6 Generic immunity part 1 Mains harmonics EN 61000 3 2 Quality System Designed and manufactured in accordance with an British Design Registration No US Design Patent No ISO9001 approved quality system 2097049 D479 879S Specifications are measured at a constant ambient temperature and are typical of a production unit As part of our policy of continuous development we reserve the right to alter specifications without notice Warranty Your supplier guarantees that the product supplied has been thoroughly tested to ensure that it meets its published specification The warranty included in the conditions of supply is valid for 12 months only if the product has been used according to the instructions supplied They can accept no liability for loss or damage however caused arising from the faulty or incorrect use of this product Issue 06 12 2003 Ultrospec 2100 pro English 27 This product has been designed and manufactured by Biochrom Ltd 22 Cambridge S
14. and F1 this enables the identification of slope start and end times for example e To go back and change the parameters press Method F1 NOTE This mode should be used to check sample stabilisation prior to kinetics studies for example since the xenon lamp is not a continuous output source unlike deuterium and tungsten lamps Reaction Rate 2 3 Reagent test kits are routinely used for the enzymatic determination of compounds in food beverage and clinical laboratories by measuring NAD NADH conversion at 340 nm The change in absorbance over a specified time period can be used to provide useful information when an appropriate factor defined in the reagent kit protocol is applied Note that reaction rate and enzyme activity can be calculated if the factor used takes account of the absorbance difference per unit time as opposed to the absorbance difference per se The correlation quality of line fit is calculated from 10 equally spaced absorbance time points during the course of the experiment The procedure is as follows Enter appropriate wavelength and press OK F3 Select time units seconds 1 or minutes 2 Enter delay time or lag time if applicable and press OK F3 Enter the duration of the assay and press OK F3 Enter factor required to convert slope to meaningful units and press OK F3 Insert reference and press green run key This reference value is used for subsequent samples until changed
15. and reporting the results the calibration interval for GLP however is always on and can be done automatically at pre defined time intervals always on daily weekly monthly quarterly If GLP is on the results are printed automatically after calibration they can also be printed on demand using Print F2 on the System page Note that the GLP print out will show the date for when the full calibration was done Calibrated and that this can be different to the date of instrument operation Date this is shown on the example below If the date is the same Calibrated shows the time that it was done instead Press More F3 on the system page to view the GLP results on the instrument display Ultrospec 2100 pro GLP Report Instrument Ultrospec 2100 pro Operator A T Dadd Date 22 September 2000 Time 10200817 Serial No 79500 Version 4190 V1 0 Calibrated 21 September 2000 Instrument Life 25 6 Hours Service 10 September 2000 Bandwidth 2 0 3 0nm 229 PASS Wavelength Accuracy 881 9nm 1 nm 881 9 PASS Absorbance Accuracy 220nm 1 763 1 781A 1 772 PASS 340nm 1 633 1 665A 1 649 PASS 500nm 1 477 1 491A 1 484 PASS Stray Light 220 nm lt 0 05 0 021 PASS Language 5 Select language for the display and print out Service 6 This is for accredited service engineers only and requires the entry of a pass code Issue 06 12 2003 Ultrospec 2100 pro English 15 Output to Print
16. are holder Temp range 20 105 C Used for DNA RNA denaturing studies Requires Temperature Control Unit 80 2105 49 Insert into socket 3 Issue 06 12 2003 Ultrospec 2100 pro English 19 Other Accessories consumables etc Description Part number Comments 80 2112 15 Use if a large number of samples for single readings is required Requires single cell holder 80 2106 05 or 80 2106 13 10mm flowcell and tubing supplied together with separate user instructions Temperature 80 2105 49 Required to supply the extra power Control Unit required by the 6 position Peltier heated cell changer 80 2106 04 and Tm Programmable heated cell holder 80 2106 14 For portable PC Printer stand 80 2112 13 For Seiko DPU 414 thermal printer Dust cover 80 2106 19 Spare Consumables and other items Pump head tubes 6 for Sipper 80 2080 74 PTFE flowcell tubing with connectors 80 2055 13 Replacement flowcell including tubing 80 2080 60 Autosampler Interface kit 80 2 104 96 Serial interface cable for connection to PC 80 2 105 97 D9 male instrument to D9 PC Spreadsheet Interface Software 80 2110 73 Centronics parallel printer interface cable 80 207 1 87 Separate information giving details on serial and parallel interface connections if required is available from a Service Engineer with your local supplier whom you should contact for further details SWIFT H Applications Software SWIFT II comprises appli
17. avelength into a concentration by a simple multiplication of absorbance x factor The procedure is as follows e Enter appropriate wavelength and press OK F3 Enter known factor range 0 01 9999 and press OK F3 e To enter a negative factor press F1 the reference should have a higher absorbance than the samples e Insert reference and press green run key e This reference value is used for subsequent samples until changed e Insert samples as required and press e repeat as necessary e To go back and change the wavelength or factor press Method F1 6 Ultrospec 2100 pro English Issue 06 12 2003 Applications 2 Wavescan 2 1 An absorption spectrum can be obtained from your instrument this enables simple identification of peak height and position A reference scan has to be obtained first The procedure is as follows Enter start wavelength range 190 890nm and press OK F3 Enter end wavelength range 200 900nm and press OK F3 Select scan speed as appropriate slow 1 medium 2 fast 3 or survey 4 The scan speed depends on the wavelength range due to the wide range in baseline energy and this in turn affects data interval so the figures are nominal Select if the peak check table Is required if selected a table of waveleneths and absorbance maxima for up to 20 peaks can be printed out Nominal scan speed nm min e Insert reference and press green run key to obtain referenc
18. calibrated test equipment e Approved to ISO 9001 standard Choice of agreement apart from break down coverage can include Preventative maintenance Certification When using calibration standard filters insert such that the flat surface is facing away from the spring end of the cell holder User maintenance is restricted to changing the mains fuse For any other maintenance operation including fitting a replacement xenon lamp contact your local supplier Fuse Replacement 1 Switch off the instrument and disconnect the power supply cord The fuse holder can only be opened if the power supply plug has been removed and is located in the power input socket on the back panel of the instrument 2 Slide the fuse holder open by pulling at the notch 3 Place fuses 1 0A 5mm x 20mm FST into the fuse holder and slide back into position 4 Reconnect the power supply cord and switch on the instrument Fuses are not normally consumed in an instrument s lifetime If they blow repeatedly contact your supplier 22 Ultrospec 2100 pro English Issue 06 12 2003 Cleaning and General Care External cleaning Switch off the instrument and disconnect the power cord Use a soft damp cloth Clean all external surfaces A mild liquid detergent may be used to remove stubborn marks Sample compartment spillages Switch off the instrument and disconnect the power cord The cell holders baseplate and sample compartment are all coated i
19. cation modules for wavelength scanning reaction kinetics quantification multi wavelength time drive and fraction analysis and can be used to enhance the software already included on the spectrophotometer Specific application packages consist of groups of modules for general analytical purposes biochemistry and molecular biology SWIFT II LAB and for method development and quality control SWIFT II METHOD 80 2108 26 SWIFT II LAB for general analytical purposes Wavelength Scanning Reaction Kinetics Quantification Time Drive 80 2108 31 SWIFT II METHOD for method development Wavelength Scanning Reaction Kinetics Quantification Time Drive Multi Wavelength Fraction Analysis Recommended PC for proper operation For optimum performance an IBM compatible 486 or greater personal computer running Microsoft Windows 95 98 or NT is required The PC should have a minimum of SMB RAM 200Mb hard disk a 1 44 MB 3 5 inch floppy disk drive a serial mouse installed and free COMMS serial port and VGA graphics Any printer supported by Microsoft Windows 95 can be used Contact your supplier for further information Issue 06 12 2003 Ultrospec 2100 pro English 21 MAINTENANCE After Sales Support We supply support agreements that help you to fulfil the demands of regulatory guidelines concerning GLP GMP Calibration certification using filters traceable to International standards e Certificated engineers and
20. cience Park Milton Road Cambridge CB4 OFJ UK 28 Ultrospec 2100 pro English Issue 06 12 2003 Issue 06 12 2003 Ultrospec 2100 pro English 29
21. e spectrum e This reference spectrum is used for subsequent samples until changed e Insert samples as required and press repeat as necessary Press Data F3 to access data points these can be viewed by moving the cursor F2 and F1 a peak is indicated by a flag symbol e For rapid movement press 4 6 to go to left right side of the graph or 5 to go the centre Press 2 to zoom in 8 to zoom out e To go back and change the parameters press Method F1 Wavescan Issue 06 12 2003 Ultrospec 2100 pro English 7 Simple Kinetics 2 2 Simple kinetics studies to investigate the shape of an assay curve can be readily performed The wavelength of interest is entered together with the time interval at which absorbances are to be read the results are displayed graphically simulating a chart recorder output The procedure is as follows e Enter appropriate wavelength and press OK F3 Select time units seconds 1 or minutes 2 e Enter the duration of the assay and press OK F3 Enter the time interval minimum 2 maximum 60 seconds e Select if the actual absorbance time data should be printed with the results Insert reference and press green run key This reference value is used for subsequent samples until changed e Insert samples as required and press repeat as necessary To see the assay on the whole display press Data F3 to return press OK F3 e Data points can be viewed by moving the cursor F2
22. eplate plug supplied and positioning the single cell holder so that the arrow is on the front face and it locates in place Then push the finger locks backwards so that they lock into position Press the accessory button on the home page Description Cell holder 10mm pathlength 80 2106 05 S Cell holder for sample 80 2108 10 Requires magnetic flea and controller stirring Cell holder 50mm pathlength 80 2106 07 Cell holder 100 mm 80 2107 14 pathlength Ultramicrovolume cell holder 80 2106 06 Use with 5 yl cell 80 2103 68 and 70 ul cell 80 2103 69 Microvolume cell holder 80 2106 09 Cylindrical cell holder 80 2106 10 Up to 100 mm pathlength cylindrical cells Water heated cell holder 80 2106 08 10 40 mm pathlength Requires a water circulating bath Replace the front blanking plug on the cell compartment lid with the new one that is provided HPLC cell holder 80 2106 11 Flowcell volume is 8 u1 pathlength is 2 5mm Thread wires through one hole of the tube guide and attach this to the instrument base using the screws provided Replace the front blanking plug on the cell compartment lid with the new one that is provided Peltier cell holder 80 2106 13 Set required temp in range 20 49 C Insert into socket 2 Electrical cell holder 80 2106 12 Set required temperature off 25 30 37 C Insert into socket 2 Tm Programmable heated cell 80 2106 14 Supplied with SWIFT Tm softw
23. er The graphics capability of the instrument means that the following requirements for printer compatibility should be fulfilled e The printer must not be USB only style parallel Centronics is required e The printer must not be designed to work with MS Windows only GDI type these are less expensive printers and can only function when connected to a PC with the appropriate driver installed If in doubt check with the printer manufacturer Note that printer output is always in black and white even on colour printers Seiko DPU 414 1 If obtained in your country it should already be configured properly If not set software DIP SW2 to American character set Epson FX 80 Epson 9 pin 2 Includes Epson FX 850 and similar Text printer no graphics 3 Use for any class of parallel printer no graphics or accents on text are printed HP PCL 3 4 Intended for printers such as HP LaserJet IVIII 4 HP DeskJet 500 HP DeskJet 690C The printer must be HP PCL level 3 or greater HP DeskJet 700 820 and 1000 series printers do not fulfil this requirement and cannot be used Use for letter or A4 sized paper European Epson 24 pin ESC P 5 For use with Epson 24 pin dot matrix printers and older inkjet printers such as the Stylus 400 Output is automatic when the key is pressed and auto print in Preferences is on If auto print is off results can be printed on demand using the key 16 Ultrospec 2100 pro English
24. erference effects in several applications By using the equation entry facility post measurement calculations can be done automatically and the end result displayed for the operator This is a very powerful facility indeed for the busy industrial QC or environmental testing laboratory Up to 5 absorbances at different wavelengths can be measured and factors applied to them an overall dilution factor can be applied to the completed equation The procedure is as follows and is best described using an example e Write the equation out in front of you ensuring there are no syntax errors u Enter the title this will be shown with the result on the display and print out so should be descriptive see Appendix e Enter the equation see Appendix Insert reference and press green run key This reference value is used for subsequent samples until changed e Insert samples as required and press repeat as necessary e To go back and change the parameters press Method F1 Multiple Wavelength Sample 1 A Ald Copper 10 511 0 009 e Method Issue 06 12 2003 Ultrospec 2100 pro English 11 Nucleic Modes 3 Nucleic acids can be quantified at 260 nm because it is well established that a solution of DNA or RNA with an optical density of 1 0 has a concentration of 50 or 40 ug ml respectively in a 10mm pathlength cell Oligonucleotides as a rule of thumb have a corresponding factor of 33 ug ml although this does vary with base
25. leic Modes 3 Methods A 4 B 5 and C 6 System Utilities Output to Printer Seiko DPU 414 1 Epson FX 80 Epson 9 pin 2 Text printer no graphics 3 HP PCL 3 4 Epson 24 pin ESC P 5 Download to Spreadsheet Messages ACCESSORIES Multiple Cell Holder Accessories Single Cell Holder Accessories Other Accessories consumables etc SWIFT II Applications Software MAINTENANCE After Sales Support Fuse Replacement Cleaning and General Care APPENDIX Text entry Good Laboratory Practice Least squares regression analysis and linearity SPECIFICATION AND WARRANTY Issue 06 12 2003 Ultrospec 2100 pro English DSDONNANARDAAUN FS WN Unpacking Positioning and Installation e Inspect the instrument for any signs of damage caused in transit If any damage is discovered inform your supplier immediately e Ensure your proposed installation site conforms to the environmental conditions for safe operation Indoor use only Temperature 10 C to 40 C Maximum relative humidity of 80 up to 31 C decreasing linearly to 50 at 40 C e The instrument must be placed on a hard flat surface for example a laboratory bench or table which can take its weight 13 kg such that air is allowed to circulate freely around the instrument e Ensure that the cooling fan inlets and outlets are not obstructed position at least 2 inches from the wall e This equipment must be connected to the power supply with the power cord supplied and
26. must be earthed grounded It can be used on 90 240V supplies e Switch on the instrument and check that the display works see Operation e To enter laboratory name operator name instrument asset number details and current date time refer to System Utilities If this equipment is used in a manner not specified or in environmental conditions not appropriate for safe operation the protection provided by the equipment may be impaired and instrument warranty withdrawn 2 Ultrospec 2100 pro English Issue 06 12 2003 Kssential Safety Notes There are a number of warning labels and symbols on your instrument These are there to inform you where potential danger exists or particular caution is required Before commencing installation please take time to familiarise yourself with these symbols and their meaning Caution refer to accompanying documents Background colour is yellow symbol and outline are black AN WARNING AN WARNING UV RADIATION UV RADIATION IS HARMFUL TO YOUR EYES HOT If power is restored with this cover removed eye protection must be worn Accessories e Care should be taken when handling all heated accessories e Ensure that the cell compartment lid is closed when operating cell changers and the sipper e It is essential that the baseplate plug supplied with single cell accessories is fitted to optimise air flow and to prevent light ingress Issue 06 12 2003 Ultrospec 2100 pro English 3 OPERATION
27. n a chemical resistant finish Strong concentration of sample however may affect the surface and spillages should be dealt with immediately Observe all necessary precautions if dealing with samples or solvents that are hazardous There is a small drain hole in the sample compartment to allow excess liquid to drain away Liquids will drain onto the bench or table under the spectrophotometer or if preferred this drain hole can be connected to waste using suitable tubing Remove the cell holder and clean separately Use a soft dry cloth to mop out the sample compartment Replace the cell holder Reconnect the power cord and switch on the instrument Issue 06 12 2003 Ultrospec 2100 pro English 23 APPENDIX Text entry The following example shows how to enter a title and equation in Multiwave The principles are identical however for other text entry options such as Method Names e To enter the title Copper 10 Use lt to remove any text still there Press 2 repeatedly until C appears oe 99 Press 6 repeatedly until o appears bbn Press 7 repeatedly until p appears Press F2 to move to next place 6699 Press 7 to enter a second p 66 99 Press 3 repeatedly until e appears Press 7 repeatedly until r appears Press 1 to initiate entry of a space Press F2 to move to next place then F2 again to enter the space Press 1 repeatedly until 1
28. o English 5 Basic Modes 1 Absorbance 1 1 Absorbance mode measures the amount of light that has passed through a sample relative to a blank this can be air The procedure is as follows e Enter appropriate wavelength and press OK F3 e Insert reference and press green run key The cell changer if fitted automatically moves to position 2 and displays the result for the reference measurement 0 000 Xenon lamp based instruments are press to read whereas deuterium tungsten lamp instruments measure continuously Thus to monitor sample stabilisation the simple kinetics mode must be used e This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary e To go back and change the wavelength press Method F1 Transmission 1 2 Transmission mode measures the amount of light that has passed through a sample relative to a blank this can be air but displays the result as a percentage The procedure is as follows e Enter appropriate wavelength and press OK F3 e Insert reference and press green run key e This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary e To go back and change the wavelength press Method F1 Factor Concentration 1 3 Concentration mode is used when a conversion factor is known and converts the absorbance measurement for a sample at a specific w
29. ropriate wavelength and press OK F3 Select Curve Fit method Single Point 1 Linear Regression 2 or Linear Interpolation 3 Enter number of standards 2 12 and press OK F3 Enter number of replicates 1 3 and press OK F3 Enter concentration of first standard and press F3 To include a zero concentration standard include this in the number of standards to be entered and enter 0 00 for concentration use a blank when required to enter standard 1 Enter concentrations of other standards as prompted Insert reference and press green run key This reference value is used for subsequent samples until changed Insert standards as required and press followed by OK F3 repeating as necessary to construct the standard curve Values can be written down if required Press Standards F3 to see the standard curve press OK F3 to return If im linear regression mode the values for the slope intercept and coefficient of determination are printed out Insert reference and press green run key This reference value is used for subsequent samples until changed u Insert samples as required and press repeat as necessary To go back and change the parameters press Method F1 Standard Curre CHEMA Concentration 41 91 Multiwave and Equation Entry 2 5 The measurement of Absorbance values at specific wavelengths and combining these with appropriate factors is a means of overcoming int
30. ters the method must be deleted first To delete a method e press stop to return to the home page e selectone of the three method banks press delete F2 and select the required method by pressing the appropriate number you are asked to confirm this Entry of alphanumeric characters for print outs and method names e Remove default characters if necessary using e Press appropriate key on keypad to cycle through options of lower case letter numbers and upper case letters for example pressing key 2 cycles through abc2ABC Note that a space is entered using key 1 which cycles between 1_1_ Press another key to move to next letter To enter a doubled letter eg AA or number eg 00 press gt F2 and then the appropriate key again e Delete incorrect characters using Complete entry by pressing OK F3 e An example of name entry is given in the Appendix Issue 06 12 2003 Ultrospec 2100 pro English 13 System Utilities Date 10 November 2003 GLP Calibrated 15 54 45 Time 15 55 Instrument Life 164 3 Hours Operator Service 27 February 2002 Serial Number 887654 Software Version 4190 V1 9 Copyright 1999 2001 Biochrom Ltd fi A Software developed for Biochrom Ltd by Calibration Anarksoft Ltd and P M Dickerson M A After selecting the system option F1 on the home page there is initial information including the calibration status of the instrument and the date of the last full GLP calibration see
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