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Flow Cytometry User Guide:

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1. Details Project Test JG 040407 01 Requested By Any One on 4 7 2004 The MoFlo is equipped with a 5 watt Argon Laser 488 nm Work Requested a5 W Krypton laser 350 nm 414 nm 568 nm and a 35 mW HeNe laser 632 nm The instrument can measure 10 parameters simultaneously Account to be Any One 00 0 0000 Alzheimer AB and the Aged Cere Charged You can use the Printable Version link at the top of the Project Details table to display a pop up window of the project details Then use either the Printer icon in the browser menu bar or CTRL P to print Once you have printed the form and attached it to your samples you can submit them to the Flow Cytometry Lab as usual The Lab is located in Skirball 3rd floor Lab 8 3 Checking Your Project Status You can log back into the application as often as you d like to check on the status of your projects Once the lab receives and or begins work on your samples they may update the Est Comp Date and or Status columns of your Project Queue Once a project s status has been changed to Completed it will fall off your Project Queue You can view the completed projects in your queue by de selecting the Completed box in the top right corner of the Project Queue and clicking the Update List button
2. Flow Cytometry User Guide Lab Staff http tools med nyu edu Flowcytometrylab Direct laboratory questions to Direct application questions to Facility Manager Director of Applications Management John Hirst Ph D Andrea Azzolina Phone 212 263 5907 Phone 212 263 6998 Email hirst saturn med nyu edu Project Manager Erica Berliner Phone 212 263 6263 Email somit dbapp med nyu edu Getting Started 3 Easy Steps IN ORDER TO ACCESS THIS APPLICATION AND THE SELF SERVICE FACSCALIBER MACHINE PLEASE CONTACT YOUR PRINCIPAL INVESTIGATOR LAB HEAD TO MAKE SURE THAT YOUR ACCOUNT HAS BEEN ACTIVATED IF YOU PI NEEDS INSTRUCTIONS ON HOW TO ACTIVATE ACCOUNTS PLEASE DIRECT THEM TO THE PI USER MANUAL AT HTTP WWW MED NYU EDU NYUCI RESEARCHERS SHAREDCOREFACILITIES FLOW HTML 1 Log onto the application The Flow Cytometry Lab is best viewed in Internet Explorer on a PC The application is located at http tools med nyu edu Flowcytometrylab You will need to know your Kerberos ID in order to access the application For help with this information please contact School of Medicine Customer Support Services 263 HELP 4357 Leave the Password field blank and click the Logon button You will be prompted to choose your own password After doing so you ll need to repeat the login process this time with your newly established password 2 Submitting a Project The first screen you see when you log into the application is your Pr
3. oject Queue You can return to this screen at any time by clicking on the Projects tab located at the top of every screen Click on the Create New Project button and you will be directed to the online submission form A project is equivalent to a rack of test tubes each time you deliver a set of samples you should create a new project To submit your project you must 1 Enter a Project Name use something that you ll be able to easily associate with the specific samples you are submitting 2 Include instructions and or comments about the Work Requested You can cut and paste text from an existing document or simply type something in 3 Select a Debited Account to pay for the services Charges will be automatically sent to FAME once the project has been completed by the Flow Cytometry Lab If the account dropdown menu does not include an account contact your Principal Investigator Once you click the Save button the project will be added to your queue and a summary of your request will appear on the screen The project will also appear in the Project Queue of your Principal Investigator You must print this screen and attach it to the samples you are submitting Cancer Institute Flow Cytometry Order and Resulting System Microsoft Internet aepterg X ig SYS J gt x a A 7 Search she Favorites veda amp 6 gt Y X Las a NYU Cancer Institute Flow Cytometry Facility Project

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