Five-Minute 8-plex Mouse mAb Isotyping Kit
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1. Five Minute 8 plex Mouse mAb Isotyping Kit AGRO Biosystems Cat E001 K004 A lateral flow assay for simultaneous determination of 4 mouse Ig heavy chain classes and 4 light chain types For Research Use Only Product Description The Five Minute 8 plex Mouse mAb Isotyping Kit provides a lateral flow assay for monoclonal antibody class and subclass and light chain type determination When a sample is loaded onto the cassette the antibodies will form soluble complexes with pre embedded gold conjugates These complexes are resolved and captured by class or isotype specific antibodies impregnated at different areas of the membrane As a result a red line will appear at the corresponding region with isotype indicated This kit can be used to assay for IgG1 IlgG2a IgG2b IgG3 IgA and IgM along with lambda and kappa light chain types Kit Components The kit contains 5 Cat E001 K004 5 or 20 Cat E001 K004 20 pouches with 2 cassettes in each pouch One cassette is for assessing IgG1 IgG2a IgG2b IgG3 and the other one is for Kappa Lambda IgA and IgM Please note that a control C line is included in both cassette Storage Please keep the strips in their original packaging at room temperature This product is stable for up to 18 months from the certified date Please do NOT freeze the strips Sample Preparation The minimum detectable concentration is 100 ng mL Below is our recommendation on sample preparat2. io 1 100 dilution in 0 5 2 ug mL in PBS optional PBS 1 10 000 in PBS Normally ascites fluid contains multiple Igs The kit can only be used to determine the relative distribution of the main isotypes Protocol 1 Place the cassettes on a flat surface 2 Add 100 uL sample directly to the well and wait for 5 minutes 3 A red line should appear on the membrane with specific isotypes indicated at the same position A red line should also appear at the C position NOTE 1 Prepare samples before removing the strips from the sealed pouch NOTE2 Do not read after 20 minutes NOTE3 The color of tissue culture medium may cause high background To address this issue additional 40 60 uL PBS can be added into the well to clear the background NOTE4 There is no cross reactivity among isotypes Multiple red lines would indicate the presence of more than one antibody types Data N N N e To s QG a Ctrl GI G2a G2b G3 Ctrl Serial dilutions of a mouse MAb were loaded onto the isotyping cards to allow determination of its isotype The images were taken five minutes afterward Page 1 1 Contact TechSupport acrobiosystems com http www acrobiosystems com
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