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Sorvall Manual

1. CITY STATE ZIP INSTRUMENT SERIAL NUMBER ROTOR SERIAL NUMBER PART PART NUMBER HAZARDOUS CONTAMINANT S DECONTAMINATION DATE DECONTAMINATION METHOD S DECONTAMINATION CERTIFIER S SIGNATURE DATE DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing instructions on reverse PLEASE PRINT TITLE POSITION DECONTAMINATION CERTIFIED BY PHONE FAX DEPARTMENT INSTITUTION ADDRESS CITY STATE ZIP INSTRUMENT ROTOR PART SERIAL NUMBER SERIAL NUMBER PART NUMBER HAZARDOUS CONTAMINANT S DECONTAMINATION METHOD S DECONTAMINATION CERTIFIER S SIGNATURE DECONTAMINATION DATE DATE INSTRUCTIONS When an instrument that has been used with radioactive patho genic or otherwise hazardous materials requires servicing by Thermo personnel either at the customer s laboratory or at Thermo facilities the following procedure must be complied with to insure safety of our personnel 1 The instrument or part to be serviced shall be cleaned of all blood and other encrusted material and decontaminated prior to servicing by our representative No radioactivity shall be detectable by survey equipment 2 ADecontamination Information Certificate shall be completed and attached to the instrument or part If an instrument or part to be serviced does not have a Dec
2. 3 7 Controls Indicators and Displays SORVALL Centrifuges MENU Key and OPTIONS OPTION QC m RUN SLOW START SLOW wm STOP OFF STEP RUN The MENU key accesses advanced feature options listed above it plus other features such as saving parameters to memory After pressing MENU pressing 1 accesses a secondary screen to view or enter values plus confirm option selection pressing 0 deselects options pressing ENTER steps through options without changing selections or settings and on secondary screens enters values to select the option Pressing MENU again exits the options and returns to the screen without entering selecting a flashing value Automatic logging of QC RUN and daily run data is possible using WatchLog Network software packaged with the optional network computer interface package on a dedicated computer e QC RUN allows simple automatic quality control run speed temperature verification when used with the optional computer interface package The QC RUN feature will run and document the data from each set of parameters that has been saved in program memory When QC RUN is selected the SET screen will prompt you to prepare for the run and press START to begin the centrifuge will start running program number 1 and systematically run each program in order through program number 9 While the QC RUN is in progress the parameters of the program that is running will be in the
3. 4 Prepare two equal loads of test fluid similar in volume to normal run samples equilibrated to the desired sample temperature check the sample temperature using a cali brated thermometer such as a Fluke 51 K J or equivalent NOTE fluid must be compatible with aluminum and have a freezing point below the desired sample temperature 5 Load and balance the rotor according to the instructions in the rotor manual then close the chamber door 6 Press START during the run the RUN display tempera ture should be within 2 of the SET display temperature RC12BP WARNING Do not open the chamber door when the rotor is spinning In the event of a power failure or any failure where RUN speed does not register the brake will not operate and the rotor will coast to a stop from high speed deceleration to 0 could take as long as 30 minutes Before using the mechanical override to open the chamber door use the viewing port in the door to make sure that the rotor has stopped spinning Opening a chamber door when a rotor is spinning exposes hazardous energy contact with a spinning rotor could cause personal injury FRONT CABINET PANEL Maintenance 7 After termination immediately after the rotor stops and the door unlocks open the chamber door and measure the sample temperature using the same calibrated thermometer that was used earlier The measured sample temperature should be within 2 C of S
4. Before using the RC72BP for the first time we recommend that you read all WARNINGS and CAUTIONS as well as Chapter 3 to familiarize yourself with the centrifuge controls and features Turning the Centrifuge ON To turn the RC 2BP ON find the main POWER switch located on a small recessed panel in the upper right corner of the front cabinet panel then rock the POWER switch up to the I ON position watch the control panel LEDs to be sure that all segments light NOTE if any LED segments do not light note which ones and contact a local Thermo Service Representative Contin ued operation when the centrifuge is unable to display all run information correctly could mislead an unaware observer During start up in addition to lighting all LEDs the centrifuge microprocessor performs a short diagnostic routine and the SET display will appear as follows If an error is detected an error message will appear in the SET display If no error is found the SET display will automatically display the HOME screen that was displayed when the centrifuge was turned OFF OPTIONS selections will also be the same as when the centrifuge was turned OFF NOTE Do not attempt to open the door unless End is in the SPEED display Doing so when the interlock is to be moving could cause the interlock to bind requiring cy cling the main POWER switch several times 4 1 Operation SORVALL Centrifuges Rotor Installation Loading an
5. Thermo is not includes a ground wire with 50 A circuit protector on each positive responsible for incorrect installation power lead Installation SORVALL Centrifuges Single phase RC 2BP Centrifuges are equipped with a three wire cord with three prong connector to fit a NEMA 6 50P 50A 2 pole and earth 1 receptacle or on 230V 50Hz centrifuges a CEE 17 63A 2 pole and earth 10 receptacle 230V 50Hz polyphase RC1I2BP Centrifuges are equipped with a four wire cord with five pin connector to fit a CEE 17 32A 3 pole neutral and earth 30 receptacle This cord may be changed to meet local electrical code requirements the green and yellow wire is the ground and must be connected to the centrifuge frame 50 AMP CIRCUIT PROTECTOR WALL OUTLET MEASURED LINE VOLTAGE 50 AMP CIRCUIT PROTECTOR Figure 2 1 RCI2BP M Electrical Requirements Location NOTE RC12BP is to be installed in a Pollution Degree 2 environment at an altitude not exceeding 2000 meters 6560 feet and is a Class A product not intended for home use operation in a domestic environment could cause radio interference N CAUTION Locate the 2 a level floor For the centrifuge to function Locate the centrifuge on a properly ambient temperature and air circulation are very important level floor to avoid rotor imbalance To ensure free air circulation the centrifuge must be positioned so that during operation n
6. functionality so that set parameters cannot be changed START STOP and CLEAR are the only active keys e The lower position RUN ANY PROGRAM makes it so that users may only perform runs using run parameters recalled from program memory only RECALL the numeric keypad including ENTER and CLEAR START and STOP are active Use of the KEYPAD LOCK may offer a considerable advantage improving process control by reducing the possibility for operator error as well as restricting unauthorized run parameter or program memory changes 2 Controls Indicators and Displays Primary Function Keys and HOME Screen Fields The Primary Function Keys are the keys below the SET display Each key is positioned below a corresponding field in the HOME screen allowing simple direct access to basic run parameter controls ROTOR reserved for future use would be pressed to specify a different rotor than the one shown in the SET display which would be necessary if the rotor or bucket carrier system installed in the centrifuge were to change Because there is only one rotor that is compatible with the centrifuge at this time the ROTOR key presently has no function As future rotors are added they will be listed by rotor name and the ROTOR key would be pressed to scroll through the compatible rotors for selection Correct identification of an installed rotor is required to establish set SPEED limits correctly calculate RCF and accura
7. HOLD ACE field press TIME HOLD or ACE to select a run duration control method and if you did not select HOLD input a desired termination value in minutes and seconds up to 99 99 orin ACE up to 9 99e30 9 99 x 10 Always be sure of the control method and value before starting the run c Ifyourequirea change to the DEG C field press TEMP and input the desired sample temperature between 10 and 40 Read the sections regarding Temperature Control and Rotor Temperature Equilibration found on pages 4 11 and 4 12 4 Ifusing WatchLog Network run logging software be sure it is running then press START START indicator will blink then stay lit once the rotor starts to spin The rotor will accelerate to the set run parameters and continue until run termination At termination the STOP indicator will light and the rotor will decelerate to a stop After it stops START and STOP indicators will turn off and End will appear in the RUN TEMP display and remain there until the door is opened NOTE Do not attempt to open the door unless End is in the SPEED display Doing so when the interlock is to be moving could cause the interlock to bind requiring cy cling the main POWER switch several times Operation SORVALL Centrifuges Programmed Operation A Storing Selections to Program Memory 1 Establish all desired run parameters as explained in steps 1 and 3 of the previous section Normal Operation NOTE if you pl
8. battery has gone dead Press CLEAR turn the main power switch OFF and back ON to see if the fault recurs then contact Thermo Service Until the condition is repaired the centrifuge will not com municate to WatchLog Network logging run data will not occur but the centrifuge should still be operational If programmed operation is desired resave the run parameters but leave the main power switch ON until the condition is repaired so that saved programs are not lost When START was pressed a rapid increase in rpm was detected that could occur if no rotor was installed and the run was terminated immediately After the door unlocks install a rotor and restart the run In future use if rotors are added If a rotor was installed check to be sure that it was correctly identified in the SET display If not either change the rotor or change the setting so that they match then restart the run If a correctly identified rotor was installed it indicates either that the drive shaft is damaged or that a tachometer problem exists Remove all sample from the rotor unplug the centrifuge and contact Thermo Service WatchLog Network record NO ROTOR A momentary power failure occurred during the run and the rotor speed was still within 200 rpm of SET speed when the power was restored The automatic restart feature resumed the run in progress WatchLog Network does not docu ment this occurrence A power failure occurre
9. decontamination procedures if exposure occurs In the future if biocontainment products are added the use of sealed rotors buckets and or sample containers will offer increased protection from contamination during routine operation However these items will not guarantee protection from accidents resulting from damage to the rotor or buckets Do run hazardous materials in the centrifuge unless placed in a biohazard enclosed and operated using all appropriate safety precautions Never use any materials capable of producing flammable or explosive vapors or creating extreme exothermic reactions Use SORVALL rotors only Use of another manufacturer s rotor can cause rotor failure which could result in personal injury or centrifuge damage Never exceed the maximum rated speed of the installed rotor to do so can cause rotor failure Always reduce derate rotor speed as instructed in this manual whenever the rotor speed temperature combination exceeds the solubility of the gradient material and causes it to precipitate the compartment load exceeds the maximum allowable compartment load specified See Chapter 4 page 4 14 Failure to reduce rotor speed under these conditions can cause rotor failure Centrifuges routinely deal with high energy levels and could move suddenly in the unlikely event of rotor failure During centrifuge operation never lean or move the centrifuge keep the surrounding area clear of objects including all ha
10. rotor stops spinning and door unlocks Pressing START when a run is in progress resets run duration The red STOP key is used to terminate a run When stop is pressed or whenever a run terminates and deceleration begins the red LED status indicator light on the key will be ON and will remain ON until the rotor stops spinning and door unlocks When that happens the indicator light is turned OFF and End will appear in the RUN Temperature display to prompt the user to open the chamber door and remove sample NOTE The START and STOP keys are usable only when the HOME screen is in the SET display If any other screen is in the display the keys are disabled 3 13 Controls Indicators and Displays SORVALL Centrifuges 3 14 SET Display Advisory Messages When the RC 2BP detects a system fault or a situation requiring operator action an advisory message appears in the SET display and an alarm will sound to alert the operator In most cases pressing CLEAR shuts off the alarm and removes the message from the display if not in almost all other cases turning the main POWER switch OFF and back ON will do so The messages can be divided into two types Problem Conditions and Terminal Conditions Possible messages are listed here by message type sorted alphabetically with a description of the condition and the appropriate action Problem Conditions Problem Condition messages alert the user to a situation that will not
11. terminate a run in progress except for ROTOR IMBAL ANCE NO ROTOR or POWER FAILURE but may prevent a run from starting In many cases a Problem Condition will force action to be taken but can be remedied by the user START was pressed but the chamber door had not been opened since the previous run End will remain in the RUN Temperature display until the door has been opened The centrifuge will not start Open the door if you had not accessed the chamber since the last run remove previous sample and reload the rotor then close the door and press START If the message reappears when START is pressed it indicates a door switch failure remove all sample from the rotor unplug the centrifuge and contact Thermo Service F E START was pressed but the centrifuge did not detect that the chamber door was closed The centrifuge will not start Close the chamber door and press START If the message then appears when the door is closed it indicates a door switch failure In that case remove all sample from the rotor then unplug the centri fuge and contact Thermo Service A memory problem was discovered during the centrifuge startup routine All run information that had been saved to program memory has been lost and overwritten with SPEED 400 rpm 00 00 DEG C240 Options OFF RC12BP Controls Indicators and Displays A MEMORY FAILURE fault could indicate a processing problem but typically means that the
12. the rotor input 1500 rpm if you are preheating the rotor input the rotor s maximum rated speed the maximum rated speed of an H 12000 rotor is 4700 rpm 6 Press HOLD to select a continuous run 7 Press TEMP and enter the desired rotor temperature 8 Press START to begin rotor temperature equilibration The centrifuge will alert you that a sample overtemperature or under temperature condition exists and that pre cooling or pre heating is taking place press CLEAR 9 Monitor progress of the value in the RUN TEMPERATURE display and note the time when the displayed RUN temperature reaches the SET temperature From when it does allow the run to continue for an additional 30 minutes then press STOP 4 13 Operation SORVALL Centrifuges Running Hazardous Material WARNING The 2 Centrifuge is designed with a refrigeration system If using pathogenic toxic operates independently of the laboratory environment Because or otherwise hazardous materials in the centrifuge chamber 15 not designed for biocontainment some the RC12BP we recommend that vapors or aerosols released from uncapped leaking or broken tubes the centrifuge be located in a biohaz may leak from the chamber during operation Once a run is completed ard safety enclosure and operated the chamber door is opened the vapors or aerosols which have with all appropriate safety precau concentrated in the chamber will be released into the l
13. unsafe practice that could result in damage of equipment NOTE highlights essential information WARNING and CAUTION are accompanied by a hazard symbol and appear in the left sidebar near the information they correspond to IBM is a registered trademark of International Business Machines Corporation Pentium is a registered trademark of Intel Corporation Microsoft MS DOS Windows Windows 95 and Windows NT are registered trademarks of Microsoft Corporation 1998 1999 by Thermo Scientific RC12BP Table of Contents Important Safety Information Certain potentially dangerous conditions are inherent to the use of all centrifuges To ensure safe operation of this centrifuge anyone using it should be aware of all safe practices and take all precautions described below and throughout this manual N WARNING When using radioactive toxic or pathogenic materials be aware of all characteristics of the materials and the hazards associated with them in the event leakage occurs during centrifugation If leakage does occur neither the centrifuge nor the rotor can protect you from particles dispersed in the air To protect yourself we recommend additional precautions be taken to prevent exposure to these materials e g use of controlled ventilation or isolation areas Always be aware of the possibility of contamination when using radioactive toxic or pathogenic materials Take all necessary precautions and use appropriate
14. was Electrical Requirements NOTE The centrifuge should be connected to an Overvoltage Category II circuit and should have a means of power interruption at a remote location The RCJ2BP has specific power requirements and must be con nected to the correct supply for proper performance The nameplate on the back panel of the cabinet specify a voltage and frequency corresponding to one of the following AC supplies 200 V 50 Hz 50 A single 200 V 60 Hz 50 A single 0 220 V 50 Hz 50 A single 0 208 V 60 Hz 50 A single 230 V 50 Hz 50 A single 0 220 V 60 Hz 50 A single 0 230 V 50 Hz 32 A P poly 230 V 60 Hz 50 A single 240 V 50 Hz 50 A single 0 240 V 60 Hz 50 A single 0 The supply voltage should be checked with a voltmeter then you should verify that the voltage indicated on the nameplate on the back panel is in agreement with the measured line voltage CAUTION x T Ifthe measured line voltage is not within 10 of the voltage specified am Thecentiige onthe nameplate do not connect the power cord and operate the aged if it is connected to the wrong voltage Check the voltage listed on centrifuge or damage to the centrifuge may result Contact Thermo or the nameplate and measure the volt Your local representative of SORVALL products to see if the age at the power source before plug Centrifuge can be modified for your voltage The centrifuge system ging in the power cord
15. 0 or ENTER Min Value Set 2 ENTER CHANGE UNDERTEMPERATURE LIMIT 5 B or ENTER D0 NOT CHANGE gt ENTER MINIMUM TEMPERRTURE 868 0 or ENTER Max Ualue Set 2 ENTER SELECT STEP RUN C 1z2VES NOs ENTER DO NOT CHANGE ENTER STEP RUN SEQUENCE 062858 0 or ENTER 2 5 PROG dis ENTER SAVE TO PROGRAM MEMORY C 1 VES NO ENTER DO NOT CHANGE ENTER PROGRAM B CSINGLE RUNS 1 9 STEP RUNS 10 15 0 or ENTER ENTER NOTE Pressing MENU again will return to the HOME SCREEN from anywhere in the advanced feature option screen sequence Figure 4 1 Advanced Feature Option Screens 4 7 Operation SORVALL Centrifuges Use specific options as follows The QC RUN feature is considered usable only if the centrifuge is equipped with the optional computer interface package The connected computer should have WatchLog Network soft ware running before starting a QC RUN When QC RUN is selected the SET screen will prompt you to prepare for the run and press START to begin When START is pressed the centrifuge will start with program number 1 and systematically step through each successive set of saved param eters until it has run every program saved to PROG positions 1 9 programs will be skipped if they are programmed to factory settings SPEED 400 rpm TIME 00 00 and TEMP 40 QC RUN data will automatically be recorded by the WatchLog Netwo
16. CE ACTIVITY FREQUENCY Inspect for general cleanliness Once a week Clean the rotor chamber As required and immediately after a spill Wash the drive spindle When the rotor is removed Clean the cabinet panels As required and after a spill Defrost the rotor chamber When ice buildup occurs Check the door gas springs Once a month and brackets Check speed timer and Twice a year or as procedures dictate temperature controls Have the motor gyro inspected a year when centrifuge is serviced ground continuity tested the condenser cleaned the latch inspected and the imbalance detector checked Inspection and Cleaning a Inspection Inspect the centrifuge each week for encrusted biological deposits and general cleanliness b Cleaning Rotor Chamber The rotor chamber should be kept clean and if condensation builds up should be wiped dry to maintain efficient temperature control When necessary the rotor chamber may be washed using a mild nonalkaline detergent such as dishwashing liquid Rinse with water and dry with a soft absorbent cloth 5 1 Maintenance CAUTION Chlorides are extremely harmful to aluminum alloy rotors and can cause stress corrosion cracking Therefore if chlorides are used to disinfect the chamber be sure to rinse the chamber thoroughly with water to remove all of the chloride cleanser WARNING The door gas springs must be checked periodically for proper functioning I
17. ET temperature If required repeat this procedure at other speed temperature ranges commonly used in your run protocols If an out of range temperature 1 observed re equilibrate the chamber rotor and test fluid temperatures to match desired sample temperature and repeat the test run Emergency Sample Recovery If a power failure or malfunction that disables the door unlock function at zero speed occurs while the rotor is spinning a means to mechanically override the door interlock is provided to allow sample recovery This procedure should never be used routinely or while the rotoris spinning and is intended for emergency sample recovery only Read the WARNING To perform the mechanical override refer to Figure 5 1 1 BEZEL Turn the main power switch OFF unplug the centrifuge then use the viewing port and make sure the rotor has stopped spinning before proceeding Access the interlock motor located behind the bezel that frames Main POWER switch and KEYPAD LOCK panel in the upper right side of the front cabinet panel Remove the four screws that secure the bezel then remove the bezel MOTOR SHAFT OVERIDE KNOB SWITCH AND KEYPAD LOCK PANEL Figure 5 1 Location of Mechanical Door Override 5 5 Maintenance SORVALL Centrifuges WARNING Because of the charac teristics of the samples likely to be processed in this centrifuge biological or radioactive contamination may occ
18. P COOL If the temperature is not within 2 C asa program is completed the centrifuge will automatically repeat that step of the QC sequence That program will continue repeating until observed temperature is within 2 C As each program is completed WatchLog Network will record the observed conditions from the completed program in the database If STOP is pressed during the QC RUN sequence WatchLog Network will record the data from the program running at the time STOP was pressed but will label that data incomplete After the rotor decelerates to zero and the door is opened the QC RUN option will return to a deselected condition and the LED status indicator will no longer be lit This screen allows you to select a starting rotor acceleration rate to 250 rpm thatis more gentle than normal maximum accelera tion If SLOW START is selected when START is pressed the rotor will accelerate at a more gradual rate up to 250 rpm then transition to normal full acceleration to SET speed After selecting the SLOW START option entering 1 10 selects one of ten available starting rates with 1 being the slowest acceleration and each successive setting incrementally faster up to asetting of 10 After SLOW START selections are completed SELECT SLOW STOP will appear in the display This screen allows you to select a slow stop deceleration rate from 500 rpm that is more gentle than a normal full braking stop If SLOW STOP is selecte
19. Phase Polyphase Supply Power Receptacle Standard 230 V 50 Hz 10 230 V 50 Hz 30 Parts Supplied 517 kg 1139 16 83 8 cm 33 inch 106 7 cm 42 inch 112 8 cm 44 4 inch 244 cm 96 inch 21 8 cm 8 6 inch 170 4 cm 67 1 inch 90 2 cm 35 5 inch 66 8 cm 26 3 inch 200 V 60 Hz 10 208 V 60 Hz 10 220 V 60 Hz 10 230 V 60 Hz 10 240 V 60 Hz 10 200 V 50 Hz 10 220 V 50 Hz 10 230 V 50 Hz 10 CE 230 V 50 Hz 30 CE 240 V 50 Hz 10 50A 32 A phase NEMA 6 50R CEE 17 63A 2 pole and earth 10 CEE 17 32A 3 pole neutral and earth 30 Parts supplied with your RC 2BP Centrifuge include Operating Instructions Manual RC 2BPTM Condensed Operating Instructions RC 2BP Program Log Pad RC3BP amp RCI2BPTM Wrench Open End 9 16 inch Preinstallation Kit supplied separately Refer to Table 4 1 on page 4 2 for rotor information For more information refer to a current SORVALL Product Guide or contact Thermo or your local representative for SORVALL products Introduction amp Description SORVALL Centrifuges 1 6 Optional Computer Interface Package An optional Network Computer Interface Package Catalog No 77017 for 100 120Vac Catalog No 77018 for 200 240Vac is available for use of the QC RUN feature and automatic run data logging The requirements for the user supplied computer are A dedicated one per 16 centrifuges IBM compatible computer with Pe
20. QC RUN the message and alarm are disabled and the program is repeated until temperature is within 2 of set 3 10 HO T2BP M Controls Indicators and Displays e CHANGE UNDERTEMPERATURE LIMIT allows changing the minimum allowable sample temperature to establish a new undertemperature offset value The centrifuge calculates the difference between the set and the minimum temperatures and retains that value as an offset to apply to future runs until it is changed The retained offset will apply to any normal manual entry runs as well as to any recalled program runs specific undertemperature limit settings cannot be saved in run programs EXAMPLE See the EXAMPLE at left If you have a set temperature of 22 and set the minimum temperature to During a run if the calculated sample temperature in the RUN 20 an undertemperature alert will display goes below the minimum allowable sample temperature occur if the calculated sample a SAMPLE TEMPERATURE UNDER LIMIT message will temperature goes down to 19 If the i ispl d 1 d Thi B ttempor turee len changed 164 appear in the SET display and an alarm will soun is may fe indicate a condition requiring simple corrective action or it the minimum temperature setting will um automatically change to 2 based could indicate a refrigeration problem see SAMPLE the previously establishedoffset value TEMPERATURE UNDER LIMIT on page 3 16 This undertemperature alert i
21. RC 12BP User s Manual m SCIENTIFIC OPERATING INSTRUCTIONS SORVALE RC12BP High Capacity Lowspeed Refrigerated Centrifuge Thermo Scientific Asheville North Carolina S A SORVALL Issued February 2007 ii SORVALL Centrifuges This manual is a guide to the use of the SORVALL RC12BP High Capacity Lowspeed Refrigerated Centrifuge Information herein has been verified and is believed adequate for the intended use of the centrifuge Because failure to follow the recommendations set forth in this manual could produce personal injury or property damage always follow the recommendations set forth herein Thermo Scientific does not guarantee results and assumes no obligation for the performance of centrifuges or other products that are not used in accordance with the instructions provided This publication is not a license to operate under nor a recommendation to infringe upon any process patents Publications prior to the Issue Date of this manual may not contain data in apparent conflict with that provided herein Please consider all data in this manual to be the most current WARNING CAUTION and NOTE within the text of this manual are used to emphasize important and critical instructions WARNING informs the operator of a hazard or an unsafe practice that could result in personal injury affect the operator s health or contaminate the environment CAUTION informs the operator of an
22. SET display At the end of each program the data from that segment of the QC RUN sequence will be written to the QC RUN log If STOP is pressed while a QC RUN is in progress the data collected up through the last program completed before termination will be logged data from the program that was in progress will be logged and labelled incomplete If a system fault occurs while a QC RUN is in progress other than a computer failure disabling WatchLog Network the data would be logged as if STOP was pressed and a fault type will be recorded RC12BP Controls Indicators and Displays If during the QC RUN an observed RUN display temperature is not within 2 C of SET temperature as could be the case when there is a significant temperature change with a short run the centrifuge will continue to repeat that step of the QC RUN sequence until the observed temperature is within range The data will not be logged until the programmed run is completed successfully at which time the centrifuge will move on to the next set of programmed parameters NOTE if you plan on using the QC RUN feature consider the following as you save runs to program memory QC RUN programs should not use HOLD Programs with factory default settings of SPEED 400 rpm 00 00 and 40 will be ignored in the QC RUN sequence e Although you may choose otherwise to minimize the time it takes to perform a QC RUN we recommend the following
23. Starting with program 1 save runs to program memory in order sorted first by temperature highest first then if there is more than one run at a single temperature by speed lowest first FOR EXAMPLE The programs at right are listed in correct order for using QC RUN PROG 5 with factory default settings will be ignored by the centrifuge when performing the run program values shown are for example purposes only actual set parameters will vary While the QC RUN sequence is in progress the centrifuge will output to a connected computer the observed speed and temperature along with the SET speed and temperature for automatic data logging and simple performance verification e SLOW START chooses gentle acceleration from 0 to 250 rpm acceleration transitions to the normal maximum rate at 250 rpm with the slow start rate defined by selection of one of ten different acceleration profiles The profiles are numbered for ease of selection with number 1 being the slowest most gradual rate and each successive rate being incrementally faster up to number 10 e SLOW STOP chooses gentle deceleration from 500 to 0 rpm normal deceleration braking from set speed transitions to the more gradual rate at 500 rpm with the rate defined by selection of one of ten different deceleration profiles The profiles are numbered for ease of selection with number 1 being the slowest most gradual rate and each successive rate incorporati
24. W STOP selection will be ignored f the BRAKE OFF transition speed is set below 500 rpm the centrifuge will decelerate with full braking to 500 transition to the specified SLOW STOP rate then change to a coasting stop when the specified BRAKE OFF transition speed is reached The 12 has sample overtemperature protection that alerts the user if the calculated sample temperature in the RUN display exceeds SET temperature by more than a specified amount CHANGE OVERTEMPERATURE LIMIT allows you to specify a maximum allowable sample temperature as it relates to the current SET temperature to create a new overtemperature offset value that will be applied to future changes to set tempera ture The overtemperature limit cannot be set below 2 over the SET temperature The offset value will be retained until over written See CHANGE OVERTEMPERATURE LIMIT on page 3 10 for more information The 2 also has an automatic precool feature and this overtemperature alert feature is disabled when precooling is in progress The precool feature sounds an alarm and SAMPLE OVERTEMP PRE COOLING appears in the SET display if the calculated sample temperature in the RUN display is more than 2 C above the SET temperature either when START is pressed or during a STEP RUN when a new program s SET temperature is more than 2 lower than the RUN temperature When CLEAR is pressed the DEG C field inthe SET display will ch
25. ZBP Electrical Requirements 2 2 2 2 Centrifuge DIImietislons uoce ien itor eter rie ari edis 2 3 2 3 Front Locking Stabilizer Adjustment 2 4 3 Control Panel and Displays 3 1 4 1 Advanced Feature Option Screens 4 7 5 2 Location of Mechanical Door Override 5 5 List of Tables Table Page 4 Rotor Information 4 2 v vi HO T2BP M Introduction amp Description Chapter 1 INTRODUCTION amp DESCRIPTION This manual contains information required to operate and maintain the SORVALL 2 High Capacity Lowspeed Refrigerated Centrifuge If you require additional information regarding operation or maintenance please contact Thermo for assistance In the United States call toll free 1 800 522 7746 Outside the United States contact your local representative for SORVALL products Centrifuge Description The SORVALL RC 2BP 15 a microprocessor controlled high capacity lowspeed refrigerated centrifuge designed for use in regu lated environments such as those found in blood banking biotech nology or pharmaceutical arenas The control panel designed to be easy to use and easy to clean allows simple input through a positive feedback keypad with visual verification of set parameters and current run conditions The control panel also prompts users for correct entry and displays user messages The control pa
26. aboratory area tions For this reason when materials which are pathogenic toxic or otherwise hazardous in nature are to be run the centrifuge should be located in a biohazard safety enclosure and operated with all appro priate safety precautions Read the WARNING regarding hazardous materials found on the Safety Information Page in the front of this manual Use appropriate decontamination procedures should exposure to any hazardous material occur See Chapter 5 for the procedure to follow if a centrifuge or rotor that has been used with a hazardous material must be serviced by Thermo personnel or returned to our service facilities for repair Reducing Speed for Rotor Compartment Loads in Excess of Design Mass There is a maximum allowable compartment mass established for each centrifuge rotor see Rotor Information Table on page 4 2 To prevent rotor failure the total contents of any compartment including specimen tubes or bottles cover and any adapters or bucket liner future rotors if added may also include the mass of the bucket must not exceed the maximum allowable compartment mass unless rotor speed is reduced proportionately Strict adherence to the maximum allowable compartment mass or reduced speed see below is required to prevent rotor failure Read the WARNING on the Safety Information Page in front of this manual If the actual compartment mass total contents as explained above is more than that specified f
27. an increase of 109 or more from when the compressor first turned on The fault indicates a refrigeration system problem After the rotor stops and the chamber door is opened note if the chamber is warm if so the rotor may be too hot to touch Unplug the centrifuge and contact Thermo Service WatchLog Net work record COMPRESSOR While the refrigeration compressor was working to heat the chamber air temperature sensor detected a decrease of 10 or more from when the compressor first turned on The fault indicates a refrigeration solenoid valve problem Unplug the centrifuge and contact Thermo Service WatchLog Net work record COMP SOLENOID RC12BP Controls Indicators and Displays The refrigeration system s high side pressure sensor indi cated either an open circuit or an internal pressure above 450 psi The fault typically indicates a refrigeration system problem Possible causes include e user repairable items such as ambient air temperature too high centrifuge air inlet blocked or proper clearance not observed see page 2 2 Location e Thermo Service repairable items such as a blocked con denser condenser fan failure system overcharge or pres sure sensor malfunction Check user repairable causes before unplugging the centri fuge and contacting Thermo Service WatchLog Network record COMPRESSOR A run was in progress when the door closed switch opened indicating a circuit fail
28. an on using the QC RUN feature consider the following as you save runs to program memory QC RUN programs should not use HOLD Programs with factory default settings of SPEED 400 rpm 00 00 and 40 will be ignored in the QC RUN sequence Although you may choose otherwise to minimize the time it takes to perform a QC RUN we recommend the following Starting with program 1 save runs to program memory in order sorted first by temperature highest first then if there is more than one run at a single temperature by speed lowest first FOR EXAMPLE The programs at right are listed in correct order for using QC RUN PROG 5 with factory default settings will be ignored by the centrifuge when performing the run program values shown are for example purposes only actual set parameters will vary 2 Press MENU to access the advanced features screens then press ENTER seven times after first accessing the advanced features until the following screen is in the SET display 3 Input 1 The following screen will appear in the SET display NOTE Single digit program numbers 1 9 are for storing sets of standard run parameters double digit program num bers 10 15 are for storing step runs 3 Input a program number then press enter The SET display will then return to the HOME screen and that program number will appear in the PROG field as confirmation Record saved information on t
29. ange to P COOL which will remain in the display until the RUN temperature comes into range or the run terminates After selection CHANGE UNDERTEMPERATURE LIMIT will appear in the display 4 10 RC12BP Operation The 2 has sample undertemperature protection that will alert the user if the calculated sample temperature in the RUN display is below SET temperature by more than a specified amount CHANGE UNDERTEMPERATURE LIMIT allows you to specify a minimum allowable sample temperature as it relates to the current SET temperature to create a new under temperature offset value that will be applied to future changes to set temperature The undertemperature limit cannot be set higher than 2 C below the SET temperature The offset value will be retained until overwritten See CHANGE UNDER TEMPERA TURE LIMIT on page 3 11 for more information The 2 also has an automatic preheat feature and this undertemperature limit feature is disabled when preheating is in progress The preheat feature sounds an alarm and SAMPLE UNDERTEMP PRE HEATING appears in the SET display if the calculated sample temperature in the RUN display is more than 2 C below the SET temperature either when START is pressed or during a STEP RUN when a new program s SET temperature is more than 2 C higher than the RUN temperature When CLEAR is pressed the DEG C fieldin the SET display will change to P HEAT which will remain in the di
30. ay shows the last TIME input value HOLD shows the total MIN SEC value after deceleration to zero After an ACE controlled run ACE shows the total ACE value after deceleration to zero the termination value is in the SET display If the previous run was not controlled by ACE pressing ACE shows the ACE value at termination the SET display shows the last ACE input value Always be sure of the control method and value before pressing START TEMP is pressed to change desired calculated sample temperature C settable from 10 to 40 thatis controlled by the centrifuge during arun Thecentrifuge automatically considers the rotor selected rotor speed run time set temperature and measured temperature to calculate and maintain sample temperature during the run The calculation assumes that the sample temperature rotor temperature and SET temperature are all equal at the start of the run as they must be any time that temperature control is critical RECALL is pressed to access program memory Saved parameters are recalled by inputting a specific program number By pressing START you begin a run using the run parameters displayed Also programs can easily be recalled and modified to create new run parameters without fear of damaging the original program because information that is saved in memory cannot be overwritten by using the RECALL key modifying or replacing a program is reserved for the SELECT SAVE RUN option under the MENU key
31. backlit LCD SET display that prompts appropriate input and displays advisory messages option selection indicators and a separate large LED RUN display for across the room viewing e Programming capability allows saving to a battery backed up memory up to 9 sets of run parameters plus 6 step run se quences allowing simple recall and error free run reproducibil ity The SAVE RUN sequence alerts users before overwriting programs to eliminate inadvertent loss A convenient RECALL key allows access to saved parameters for selection or reference e STEP RUN capability allows the linking together of up to three sets of run parameters to automatically perform innovative step run protocols The step run sequences may be saved in program memory for future use e KEYPAD LOCK a3 position keyswitch with a removable key that is designed to limit control panel access Besides allowing full functionality the keyswitch can be set so that run parameters cannot be changed or so that only parameters from saved programs may be run in either case greatly reducing the chance for operator error or unauthorized changes e Runduration controlled by TIME minutes seconds by HOLD for continuous runs or by Accumulated Centrifugal Effect ACE Because rotor load differences fluctuations in voltage or slight mechanical differences affect how quickly centrifuges reach set speed ACE calculates the effect of speed in relation to time and adjusts r
32. d Balancing WARNING Install load and balance the rotor according to the instructions given If maximum compartment the rotor instruction manual mass is exceeded the maximum speed must be lowered see Reduc ing Speed for Rotor Compartment Loads in Excess of Design Mass page 4 14 Failure to do so can cause rotor failure which could result in per sonal injury or centrifuge damage Table 4 1 ROTOR INFORMATION Maximum Maximum Maximum Balance Critical Biohazard Speed RCF Compartment Margin Speed Containment Rotor rpm g force Mass grams grams rpm Available SWINGING BUCKET H 12000 1 Values reflectthe centrifuge s calculation rounding are based on the rotor s maximum radius and do not consider any tube bottle or adapter geometry 2 Maximum allowable at maximum speed see Reducing Speed for Rotor Compartment Loads in Excess of Design Mass page 4 13 3 Value includes the mass of the bucket adapter if used blood bags or bottle and sample value for contents only not including the bucket is 2160 grams Normal Operation Read the Safety Information Page at the front of this manual Check all OPTION LED indicators if selection changes required press MENU then press ENTER to access each option screen in succession see figure 4 1 on page 4 7 As you step through each screen you may select or deselect and change or accept the previous setting To check an existing selection setting S
33. d at run termination the rotor will decelerate under full braking to 500 rpm then transition to a more gradual deceleration rate to stop After selecting the SLOW STOP option entering 1 10 selects one of ten available stopping rates Entering 1 selects the slowest rate and each successive setting incorporates incrementally more braking up to a setting of 10 Particularly when establishing blood bank protocols for optimized braking with minimal resuspension we recommend starting at setting 5 and adjusting up or down as needed After SLOW STOP selections are completed SELECT BRAKE OFF will appear in the display Selection of BRAKE OFF will have an affect on SLOW STOP see the NOTE under SELECT BRAKE OFF Operation SORVALL Centrifuges This screen allows you to select a speed in rpm at which after termination deceleration will transition from braking to a coast ing stop The amount of time it takes for the rotor to stop depends on the transition speed windage and inertia of the rotor BRAKE OFF transition speed is set independently of set run speed and is not affected by changes to set run speed If the transition speed is set higher than the set run speed at run termination the centrifuge will coast to a stop from set speed After selection CHANGE OVERTEMPERATURE LIMIT will appear in the display NOTE if SLOW STOP and BRAKE OFF are both selected If the BRAKE OFF transition speed is set to 500 rpm or higher the SLO
34. d to program memory and can occupy program number 10 15 This screen allows pre programming of up to 15 different sets of run parameters 9 standard runs and 6 step runs for simple recall and error free run reproducibility of frequently used protocols To eliminate inadvertent loss of existing programs the storage sequence alerts users before overwriting The convenient RE CALL key allows browsing through existing programs for selection or reference NOTE plan to use the QC RUN feature specific guidelines should be considered when saving parameters to memory see QC RUN on page 3 8 To save arun all run parameter selections should be made before selecting this option After SAVE TO PROGRAM MEMORY selection and assignment of a program number the SET display will return to the HOME screen See Programmed Operation on page 4 4 Temperature Control The RCJ2BP controls calculated sample temperature during a run based on the rotor selected rotor speed run time SET temperature and measured chamber temperature The complex calculation the centrifuge performs must assume however that the sample and the rotor are at SET temperature at the start of the run The centrifuge makes precise calculated adjustments to control temperature of equilibrated components the control adjustments will not precisely compensate for the error introduced if a rotor or its contents are not at SET temperature at the start of a run although eve
35. d during the run and the rotor speed was not within 200 rpm of SET speed when power was restored disabling automatic restart The run in progress was terminated If power is restored before zero speed WatchLog Network record POWER FAIL If power is restored after zero speed there will be no record of the run Excessive rotor vibration was detected the run in progress was terminated Rebalance the rotor according to directions in the rotor instruction manual then restart the run WatchLog Network record IMBALANCE 3 15 Controls Indicators and Displays SORVALL Centrifuges 3 16 The RUN display temperature was more than 2 C above the SET temperature either when START was pressed or during a STEP RUN at a moment when the programmed SET temperature changed Press CLEAR to continue the run as a Precool run or press STOP to terminate the run to remove sample before precooling the chamber to SET temperature During a Precool run P COOL replaces the DEG C field header in the SET display until RUN temperature is within 2 C of SET value During a run when the pre cool feature was not active see SAMPLE OVERTEMP PRE COOLING above the RUN display temperature went above the maximum allowable overtemperature limit setting Check all possible causes a SET temperature too low for rotor speed combination b overtemperature limit too close to the SET temperature see page 3 10 CH c ambient a
36. dication of a drive fault May indicate a motor problem or if it occurs at same time as Tach Fault a tachometer problem Wait for the rotor to stop spinning this could take a few minutes then use the me chanical override to remove all sample from the rotor un plug the centrifuge and contact Thermo Service Read the WARNING on page 3 18 WatchLog Network record DRIVE The microcomputer detected either that the drive motor was not responding or that the tachometer circuit failed Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARNING on page 3 18 WatchLog Network record DRIVE DIT ees 5098 se i i H After run termination while the rotor is decelerating and the drive was working to reverse either the drive box stopped responding or a speed increase was detected Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARNING on page 3 18 WatchLog Network record DRIVE The chamber floor temperature sensor has indicated either a temperature above 55 C or an open circuit The fault indi cates a sensor failure or a refrigeration problem After the rotor stops and the chamber door is opened no
37. e or rotor must be returned to a Thermo facility 1 Contact your Thermo representative to obtain a Return Service Order Number RSO be prepared with the name and serial number of the centrifuge or rotor and the repairs required 2 Send item s with the RSO clearly marked on the outside of package to the address obtained from a Thermo representative NOTE United States federal regulations require that parts and instru ments must be decontaminated before being transported Outside of the United States check local regulations Ifa centrifuge or rotor to be serviced does not have a Decontamination Information Certificate attached and in Thermo s opinion presents a potential radioactive or biological hazard the Thermo representative will not service the equipment until proper decontamination and certification is complete If Thermo receives a centrifuge or rotor at its Service facilities which in its opinion is a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Disposition costs will be borne by the sender Decontamination Information Certificates are included with these instructions Additional certificates are available from the local Account or Service Representative In the event these certificates are not available a written statement certifying that the unit has been properly decontaminated and outlining the procedures used will be acceptable NOTE The Service Re
38. ed with the preinstalled hold down brackets 2 Securethe centrifuge to the brackets by installing a shackle and alocking bolt supplied with the kit through each tab hold down bracket eyelet and tightening 2 3 Installation SORVALL Centrifuges N CAUTION The locking stabilizers must be used as explained to lift the front caster off the floor The centrifuge can be damaged if itis operated when the stabilizers are not properly ad justed After adjusting the stabilizers make sure that the centrifuge is rea sonably level and that it does not rock on three of the four support points 2 4 3 By hand lower the two locking stabilizers in the front of the centrifuge until they lightly contact the floor Use the 9 16 inch wrench supplied with the centrifuge to rotate each stabilizer an additional two revolutions This will raise the front caster about 3 mm 1 8 inch off the floor Check that the centrifuge does not rock on its four support points the two front stabilizers and the two rear casters see figure 2 3 Read the CAUTION APPROX mm 1 1 8 inch OFF THE FLOOR LOCKING STABILIZER 9 16 inch HEX 14 mm am Figure 2 3 Front Locking Stabilizer Adjustment 4 Be sure the POWER switch is set to O then plug in the centrifuge power cord 5 Optional To be certain the centrifuge is working properly turn tothe Customer Control Inspection paragraph located in Chapter 5 Maintenance and
39. ements Location Installation eesceeeeeeen nennen Chapter 3 CONTROLS INDICATORS and DISPLAYS RUN Display SET ENSIS HOME BORO OT Main POWER and KEYPAD LOCK Primary Function Keys and HOME Screen Fields MENU Key and OPTIONS retten Numero Keypad cenae apt Oan RU RUM P START and STOP Keys Problem Conditions Chapter 4 OPERATION Turning the Centrifuge ON Rotor Installation Loading and Balancing Normal ODeratol Programmed Operation Using Advanced Features Options Temp rature Control nasi dade Rotor Temperature Equilibration SET Display Advisory Messages Terminal RC12BP Table of Contents OIII Page Running Hazardous Material 4 14 Reducing Speed for Rotor Compartment Loads in Excess of Design MASS sinisisi 4 14 Chapter 5 MAINTENANCE Insp cti n and 5 1 TUBAL OM ssion ee a UP Pe VOV DM Cd DUE SUPR Eai 5 2 Customer Control Inspection uu secedere 5 3 Emergency Sample RECOVERY 5 5 Parts Ordering Information vecta PERRA GIA RUNI SIQUIS 5 6 Service Decontamination Policy 5 7 APPENDIX Warranty Decontamination Certificates List of Illustrations Figure Page 1 1 Parts Location and Identification 1 2 2 1 RC
40. et value The centrifuge translates set RCF values to rom whole numbers for speed control purposes then calculates that rom whole number to the closest RCF value There is no cause for concern speed control accuracy is not compromised and the slight value difference will not affect the run The run duration from start to termination when deceleration begins setting or control method is changed by pressing one of the three grouped Primary Function keys e TIME is pressed to specify a length of time minutes and seconds toa maximum of 99 99 from run start to termination In the RUN display minutes and seconds begin counting down from the input value when START is pressed The run terminates and deceleration begins when the timer reaches zero e HOLD is pressed to specify a continuous run which once started runs until STOP is pressed terminating the run and initiating deceleration During a continuous run HOLD will be in the SET display and the time elapsed from when START was pressed will appear in the RUN display The timer will accumulate up to 300 minutes if a run continues beyond that displayed run time will no longerreflectelapsed time but will remain fixed at 300 00 Whenever it is possible for such an extended run to occur an external timing method is recommended TERMINATION e is pressed to specify an Accumulated Centrifugal Effect M SETPOINT value When ACE control is selected the centrifuge calculates t
41. f gas pressure is not suf ficient the door will not stay open and possible injury could result 5 2 SORVALL Centrifuges Use 70 ethanol to disinfect the rotor chamber or a 2 glutaralde hyde solution to sterilize it For general radioactive decontamina tion use a solution of equal parts of 70 ethanol water and 10 SDS Follow with ethanol rinses then deionized water rinses Dry with a soft absorbent cloth Dispose of wash solution in proper radioactive waste containers Read the CAUTION In normal operation it is not unusual for moisture in the air to condense on the cool walls of the rotor chamber so that the chamber must be wiped dry When the chamber gets colder frost can collect on the chamber walls and as it builds up act as an insulator between the chamber air and the refrigerant If frost collects on the chamber walls you should periodically defrost the chamber to maintain efficient cooling To defrost the cham ber remove the rotor close the chamber door and change the SET temperature to 25 When the displayed RUN temperature reaches 20 open the door and wipe all parts of the chamber dry Then wipe the rotor dry reinstall it close the chamber door and enter a desired SET temperature Drive Spindle Wipe the spindle with a soft cloth before a rotor is installed to reduce the chance of the rotor sticking to the spindle If neces sary to remove deposits the spindle may be washed with warm water make sure
42. fterthe centrifuge powerhas been set ON and the diagnostic test has been completed In addition if MENU is pressed to access the options screens pressing MENU again will return the HOME screen to the display The SET display is the primary interactive screen on the control panel and has a role in all aspects of centrifuge use Following is a detailed description of all HOME Screen fields as they relate to the Primary Function Key functionality followed by option selection screens and advanced feature functionality At the end of this chapter is a listing of the SET Display advisory messages what they mean and recommended action Controls Indicators and Displays SORVALL Centrifuges Main POWER and KEYPAD LOCK C N RUN SINGLE PROGRAM FULL FUNCTION RUN ANY PROGRAM KEYPAD LOCK The Main POWER and KEYPAD panel is located away from the control console in the top right corner of the front cabinet panel The POWER I O ON OFF switch is a 50 amp circuit breaker that turns the centrifuge ON and OFF by connecting or disconnecting the main supply power from all system circuitry The KEYPAD LOCK can be used to limit control panel function It uses a 3 position keyswitch with a key that is removable in any of the 3 positions Functionality is as follows e The middle position FULL FUNCTION allows use of all keys and features without restriction e The upper position RUN SINGLE PROGRAM minimizes
43. ged The retained offset will apply to any normal manual entry runs as well as to any recalled program runs specific overtemperature limit settings cannot be saved in run programs EXAMPLE See the EXAMPLE at left If you have a set temperature of 22 and set the maximum temperature to During a run if the calculated sample temperature in the RUN 24 an overtemperature alert will occur display goes above the maximum allowable sample temperature if the calculated sample temperature aSAMPLETEMPERATURE OVERLIMIT message will appear reaches 25 If the set temperature is then Ghantied 46 4 te maximum in the SET display and an alarm will sound This may indicate temperature setting will automatically acondition requiring simple corrective action or it could indicate change to 6 based on the previously arefrigeration problem see SAMPLE TEMPERATURE OVER calculated offset value LIMIT on page 3 16 This overtemperature alert is not activated and a precool mode begins if the RUN display temperature is more than 2 C over the SET value when START is pressed or when a temperature change between programs occurs ina STEP RUN In each case a SAMPLE OVERTEMP PRE COOLING message will appear and an alarm will sound Pressing CLEAR removes the message and shuts off the alarm allowing the run to continue as a precool run P COOL replaces DEG Cas the field header in the SET display during precool mode precool mode is automatic during a
44. gized when it should not have been or that the brake circuit is otherwise open Wait for the rotor to stop spinning remove sample from the rotor unplug the centrifuge and contact Thermo Service WatchLog Network record SHUNT OVERDRIVE During a run the tachometer signal was lost indicating that the tachometer circuit has failed or that the drive shaft is damaged Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARN ING on page 3 18 WatchLog Network record TACH FAULT The microprocessor has detected erroneous information or encountered a processing problem The run in progress may or may not be terminated the controls and displays might lock up Turn the main power switch OFF and back ON If the fault recurs turn the main power switch OFF Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARNING on page 3 18 WatchLog Network may not record UNEXPECTED ISR 3 21 3 22 HO T2BP M Operation Chapter 4 OPERATION This chapter provides the operating procedures for the RC72BPTM Centrifuge NOTE Before turning on the RC72BP for the first time be sure it is properly installed see Chapter 2 Installation
45. he effect of speed in relation to time the shaded area in the illustration at left adjusting run duration to account for acceleration variation This variationcan be attributed to rotor load configuration differences affecting inertia fluctuations in line voltage affecting motor power or slight mechanical differences including normal wear WhenSTARTis pressed therealized ACE value begins to accumulate TIME in the RUN display The run will terminate and deceleration will begin when the specified ACE value expressed as Jovdt Accumulated Centrifugal Effect controllable up to 9 99 x 10 displayed as 9 99e30 is reached calculating the area under the curve After termination the RUN display will continue to accumulate until the rotor stops this run total is for reference care should be taken not to confuse the RUN display s final accumulation with the controlling input timeout value foundin SET display Compared to control by time ACE selection provides a more advanced and relevant form of run duration control thereby improving separation consistency run reproducibility and dependability of results The integral value can be calculated deriving the following formula wedt w jdt At Where At Change in time seconds and w Angular speed 2n n 60 Speed rpm n 3 6 RC12BP Controls Indicators and Displays During a run to view in an alternative duration control
46. he Program Log Pad supplied RC12BP Operation NOTE To prevent accidental erasure of established programs if the entered program number has been previously assigned the following message will appear To review stored programs before overwriting refer to your Program Log Pad or press RECALL If you press RECALL you will have to go back and reestablish the run parameters before you can store them to memory B Performing a Programmed Run Read the Safety Information Page at the front of this manual 1 Press RECALL the HOME screen will change to display a program number flashing in the PROG field 2 Inputthe desired program number then press ENTER the SET display and options indicators change to reflect the run param eters that were saved to that program number 3 Prepare the rotor according to the rotor manual instructions 4 Openthe chamber door wipe the chamber and drive spindle dry if moisture is present and install the rotor making sure that the rotor is fully seated and locked to the drive spindle Install the rotor cover if any then close the chamber door NOTE Rotor names are not saved or recalled in future use if new rotors have been added be sure that the installed rotor is correct for the recalled program make sure that the SET rotor identifies the installed rotor 5 Ifusing WatchLog Network run logging software be sure it is running then press START START indicator will blink t
47. hen stay lit once the rotor starts to spin The rotor will accelerate according to the recalled parameters and continue until run termination At termination the STOP indicator will light and the rotor will decelerate to a stop After it stops START and STOP indicators will turn off and End will appear in the RUN TEMP display and remain there until the door is opened NOTE Do not attempt to open the door unless End is in the SPEED display Doing so when the interlock is to be moving could cause the interlock to bind requiring cy cling the main POWER switch several times Operation SORVALL Centrifuges Using Advanced Features Options The advanced features of the RCJ2BP allow for performing quality control runs changing speed or time control ranges changing acceleration deceleration or overtemperature alert settings linking together programmed run parameters and saving run parameters to program memory for simple recall Detailed description of each of the advanced features can be found in Chapter 3 Advanced feature selection is usually reflected by LED option status indicators when a feature has been selected the corresponding indicator will be lit although specific settings for several features can only be checked by reselecting the feature in the options screens If changes to advanced feature selection are required other than to RPM RCFor TIME HOLD ACE status which are accessible through primary function
48. hout any obligation whatever to make correspond ing changes to any instrument previously sold or shipped The foregoing obligations are in lieu of all other obligations and liabilities including negligence and all warranties of merchantability or otherwise expressed or implied in fact or by law and state our entire and exclusive liability and buyer s exclusive remedy for any claim or damages in connection with the sale or furnishing of goods or parts their design suitability for use installation or operation Thermo will in no event be liable for any special or consequential damages whatsoever and our liability under no circumstances will exceed the contract price for the goods for which liability is claimed DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing instructions on reverse PLEASE PRINT TITLE POSITION DECONTAMINATION CERTIFIED BY PHONE FAX DEPARTMENT INSTITUTION ADDRESS CITY STATE ZIP INSTRUMENT SERIAL NUMBER ROTOR SERIAL NUMBER PART PART NUMBER HAZARDOUS CONTAMINANT S DECONTAMINATION DATE DECONTAMINATION METHOD S DECONTAMINATION CERTIFIER S SIGNATURE DATE DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing instructions on reverse PLEASE PRINT TITLE POSITION DECONTAMINATION CERTIFIED BY PHONE FAX DEPARTMENT INSTITUTION ADDRESS
49. iderverwetet oder entsorgt werden Mehr Informationen ber die Einhaltung dieser Anweisungen durch Thermo Scientific die Verwerter und Hinweise die Ihnen n tzlich sein konnen die Thermo Scientific Produkte zu identizfizieren die unter diese RoHS Anweisung fallen finden Sie unter www thermo com WEEERoHS Conformit WEEE Questo prodotto deve rispondere alla direttiva dell Unione Europea 2002 96EC in merito ai Rifiuti degli Apparecchi Elettrici ed Elettronici WEEE E marcato col seguente simbolo Thermo Scientific ha stipulato contratti con una o diverse societ di riciclaggio smaltimento in ognuno degli Stati Membri Europei Questo prodotto verr smaltito o riciclato tramite queste medesime Ulteriori informazioni sulla conformit di Thermo Scientific con queste Direttive l elenco delle ditte di riciclaggio nel Vostro paese e informa zioni sui prodotti Thermo Scientific che possono essere utili alla rilevazione di sostanze soggette alla Direttiva RoHS sono disponibili sul sito www thermo com WEEERoHS Conformit WEEE Ce produit doit tre conforme la directive euro p enne 2002 96 des D chets d Equipements Electriques et Electroniques DEEE Il est merqu par le symbole suivant Thermo Scientific s est associ avec une ou plusieurs compagnies de recyclage dans chaque tat membre de l union europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Scie
50. ing run time min sec during a HOLD run displays elapsed run time min sec during an ACE run displays current Accumulated Centrifugal Effect coefficient and exponent ACE indicator lit PRIMARY FUNCTION KEYS These keys allow access to the corresponding fields of the SET display forthe specification of new run parameters ROTOR TEMP Future use To allow selec Allows changing desired cal tion of the rotor to be used culated sample temperature SPEED Allows Tinn jar selectin Allows changing desired 9 9 speed rpm or RCF gforce programmed parameters TIME HOLD ACE Allows changing desired run duration runs continue until speci fied min sec times out until STOP is pressed or until a specified Accumulated Centrifugal Effect is achieved SET DISPLAY Displays currently selected ba sic run parameters in the HOME screen advanced feature screens for selection of options or programming and user advi sory messages NUMERIC KEYPAD Inputs speed time and tem perature values selects programs and advanced features options program ming CLEAR removes an input value or a message from the SET display toggles between positive and negative for temperature value selection ENTER Places input values in memory Bypasses advanced features without selecting or deselecting them Figure 3 1 Control Panel and Displays ROTOR SPEED H12000 4700 e dg du MIN SEC TEMP PROG selecti
51. ir temperature at the centrifuge inlet 35 C d centrifuge air inlet blocked or proper clearance not observed see page 2 2 Location Check all possible causes before contacting Thermo Service WatchLog Network record OVERTEMP During a run when the preheat feature was not active see SAMPLE UNDERTEMP PRE HEATING below the RUN display temperature went below the minimum allowable undertemperature limit setting The limit setting may be too close to the SET temperature see page 3 11 CHANGE UNDERTEMPERATURE LIMIT try setting a lower undertemperature limit If the problem persists there may be a refrigeration system problem Contact Thermo Service WatchLog Network record TEMP UNDER LIMIT The RUN display temperature was more than 2 C below the SET temperature either when START was pressed or during a STEP RUN at a moment when the programmed SET temperature changed Press CLEAR to continue the run as a Preheat run or press STOP to terminate the run to remove sample before preheating the chamber to SET temperature During a Preheat run P HEAT replaces the DEG C field header in the SET display until RUN temperature is within 2 C of SET value RC12BP Controls Indicators and Displays Future use if rotors are added START was pressed but the centrifuge detected a set SPEED value that was too high for the SET rotor The centrifuge will not start This can occur if you change the SET rotor but
52. it is wiped dry before installing the rotor Cabinet The enameled cabinet panels may be cleaned with a household wax Cleaner A mild nonalkaline detergent and water may be used to clean the top deck and the chamber door Door Gas Springs Monthly check that the two door gas springs are functioning properly by opening the chamber door and making sure it remains open The gas springs counterbalance the weight of the door and hold it in the open position Also check that the connecting ends of each gas spring and the brackets they are connected to have not developed cracks If the chamber door will not stay in the open position or a gas spring or bracket is cracked contact your local Service Representative Lubrication All components are prelubricated and require no further lubrication The refrigeration unit is hermetically sealed and the ball bearings in the gyro action drive assembly and the centrifuge motor are perma nently lubricated RC12BP Maintenance Customer Control Inspection To keep your centrifuge in good working condition and ensure accurate and consistent results we recommend that you use the following procedures to check the speed control timer control and temperature control at least twice a year or more often as your procedures dictate If any of these control checks reveal a control inaccuracy contact Thermo Service or your local representative for SORVALL Products to arrange to have a qualified se
53. keys the features are accessed by pressing MENU and then pressing ENTER until the selection screen of the particular feature appears in the SET display Pressing ENTER steps through the options screen sequence without changing preexisting selection status unless a new value is input Selections are made by inputting 1 or 0 then entering any required setting values that may be prompted on a subsequent screen for that option After the last option in the options screen sequence SAVE TO PROGRAM MEMORY the HOME screen will reappear in the SET display If you wish to exit the sequence before that MENU may be pressed at any time to return to the HOME screen HO T2BP M Operation HOME SCREEN ROTOR SPEED TIMED DEG C PROG s H12800 2908 64 08 4 1 MENU START 1 PREPARE GC RUN PRESS START TO BEGIN SELECT C RUN C 1 VES or ENTER NO gt 0 or ENTER SELECT SLOW START 1 VES 8 NO EHTER DO NOT CHANGE gt ENTER ACCELERATION RRTE BO 51 10 1 SLOWEST gt 0 or ENTER ENTER SELECT SLOW STOP C1 VES 9 ENTER DO NOT CHANGE gt ENTER DECELERATION RRTE 88 0 or ENTER 51 16 1 SLOWEST gt ENTER SELECT BRAKE OFF NO3 ENTER D0 NOT CHANGE ENTER TRANSITION SPEED 88808 MAX VALUE 4708 RPM 0 or ENTER ENTER CHRNGE OUERTEMPERRTURE LIMIT C1 VES or ENTER D0 NOT CHANGE gt ENTER MAXIMUM TEMPERRTURE 00 k
54. l override to open the chamber door use the viewing port in the door to make sure that the rotor has stopped spinning Opening a chamber door when a rotor is spinning exposes hazardous energy contact with a spinning rotor could cause personal injury 3 18 SORVALL Centrifuges Terminal Conditions Terminal Condition messages alert the user to a serious fault condi tion Runs are disallowed or terminated These conditions typically cannot be remedied by the user the centrifuge should be removed from service until it is repaired by Thermo Service During a run the primary tachometer signal was indicating 2000 rpm or more while the secondary tachometer signal was indicating 0 rpm Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARN ING WatchLog Network record TACH FAULT The chamber air temperature sensor indicated either an open circuit or a temperature above 45 C The fault indicates a sensor failure or a refrigeration system problem After the rotor stops and the chamber door is opened note if the chamber is warm if so the rotor may be too hot to touch Unplug the centrifuge and contact Thermo Service WatchLog Network record AIR SENSOR While the refrigeration compressor was working to cool the chamber air temperature sensor detected
55. low Start Stop rates Brake Off speed Overtemperature Limit or Step Run sequence press to display the option s secondary screen then view the displayed value When finished with all changes press MENU to return to the HOME screen HO T2BP M Operation NOTE Do not attempt to open the door unless End is in the SPEED display Doing so when the interlock is to be moving could cause the interlock to bind requiring cy cling the main POWER switch several times 2 Prepare the rotor according to the rotor manual Open the chamber door wipe the chamber and drive spindle dry if wet and install the rotor making sure the rotor is fully seated and locked to the drive spindle Load the rotor install its cover and close the chamber door In future use if new rotors have been added make sure that the SET rotor specifies the installed rotor If not press ROTOR repeatedly until it does 3 Check the settings in all other fields in the SET display input changes to specific parameters as follows a Ifyourequireachangeto the SPEED rpm or RCF e force field press SPEED and input the desired value Values in excess of the maximum for the specified rotor will not be accepted the value will continue to blink To change mode from rpm to RCF or from to rpm press SPEED more than once an equivalent value will be calculated based on the maximum radius of the specified rotor and displayed b If you require a change to TIMED
56. mode for example to monitor accumulating TIME when an ACE run is in progress press one of the other two duration control keys once this changes the RUN display to show an accumulating value in that alternative mode without altering the way duration is controlled In this viewing condition the SET display field header does not change nor will the status of the ACE indicator both continuing to indicate the set control mode but the SET value will change to show the last value entered in the alternative mode If you press the same key a second time the run will then change to that control method using the value that was in the SET display as the controlling value until a different one is entered Pressing START resets the run NOTE if you change the duration control mode to TIME during a run time will begin to count down from the set value the moment the change is made unless the run was previ ously controlled by time and you are returning in which case the run resumes counting from the value that was remaining when TIME was exited If you change to ACE control the last set ACE value that is displayed the mo ment the change is made will control termination so that if you change to ACE when the run has already exceeded the value the run will terminate immediately Between runs the RUN display shows the previous run duration values viewable in each control mode Pressing TIME shows the MIN SEC value at termination the SET displ
57. nated as rpm 1 1 Introduction amp Description SORVALL Centrifuges The RC12BPTM has the following safety features a protective armor plate steel guard within the cabinet automatic shutoff of the drive motor for overspeed protection circuit breakers on the main power and the control panel circuits and a door interlock which prevents starting the drive while the chamber door is open or opening the door while the rotor is in motion Figure 1 1 identifies and locates parts of the 2 WatchLo 290 R BarTrace Neison RUN SCANNER CONNECTOR CONNECTOR DISPLAY VIEWING TACHOMETER PORT dL CONTROL PANEL wes RH COVER HOLDER ROTOR CHAMBER AIR OUT BOTH SIDES DRIVE SPINDLE CASTER POWER CORD AIR IN KEY SWITCH AND BREAKER LATCH OVERRIDE BEHIND BEZEL LOCKING STABILIZER Figure 1 1 Parts Location and Identification Specific RCJ2BP features include e optional Network Computer Interface Package which in cludes WatchLog Network M software allows automatic com puterized logging of centrifuge QC RUN and production run data via RS485 RS232 communication hardware e QC RUN feature allows simple automatic speed temperature verification optional Computer Interface Package required to record and print run data HO T2BP M Introduction amp Description e A user friendly easy to clean control panel with an interactive
58. nel introduces automation of quality control runs an optional computer interface package enables automatic on line quality control run and production run data logging The 2 operates at speeds up to 4700 rpm and is capable of producing relative centrifugal force up to 7333g It uses a balanced high torque brushless dc motor designed to deliver optimal performance with smooth quiet operation over its full speed range with long bearing life Advanced capabilities of this motor offer precise control of acceleration and deceleration regardless of rotor load for exceptional run reproducibility Directly connected to the motor is a heavy duty gyro action drive with a square spindle that accepts the SORVALL H 12000 high capacity swinging bucket rotor A viewing port in the chamber door permits speed confirmation using an optical tachometer Precisetemperature controlis made possible by a refrigeration system that is capable of delivering both cooling and heating The high capacity system is charged with environmentally friendly CFC free refrigerant The microprocessor controlled system consists of a low temperature evaporator a hermetically sealed thermally protected compressor and a fan cooled finned condenser Speedinrevolutions per minute rpm is related to angular velocity according to the following 2x rpm 2 rpm 0 10472 Where w rad s All further references in this manual to speed will be desig
59. ng incrementally more braking up to number 10 Particularly when 3 9 Controls Indicators and Displays SORVALL Centrifuges establishing blood bank protocols for optimized braking with minimal resuspension we recommend starting at setting 5 and adjusting up or down as needed Selection of BRAKE OFF will have an affect on SLOW STOP see the NOTE under BRAKE OFF e BRAKE OFF deactivates normal deceleration braking for a coasting stop from any specified speed in rpm between 4700 and 0 BRAKE OFF transition speed is set independently of set run speed and is not affected by changes to set run speed If the transition speed is set higher than the set run speed at run termination the centrifuge will coast to a stop from set speed NOTE if SLOW STOP and BRAKE OFF are both selected If the BRAKE OFF transition speed is set to 500 rpm or higher the SLOW STOP selection will be ignored f the BRAKE OFF transition speed is set below 500 rpm the centrifuge will decelerate with full braking to 500 transition to the specified SLOW STOP rate then change to a coasting stop when the specified BRAKE OFF transition speed is reached CHANGE OVERTEMPERATURE LIMIT allows changing the maximum allowable sample temperature to establish a new overtemperature offset value The centrifuge calculates the difference between the set and the maximum temperatures and retains that value as an offset to apply to future runs until it is chan
60. ntific ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Scientific qui peuvent aider le d tection des substances sujettes la directive RoHS sont disponibles sur www thermo com WEEERoHS CHINA Tel 86 21 68 65 45 88 86 10 58 50 35 88 AUSTRIA Tel 43 1 80 14 00 FRANCE Tel 33 1 69 18 77 77 33 2 28 03 20 00 NETHERLANDS Tel 31 7 65 7144 40 INDIA Tel 91 22 55 42 94 94 Tel 852 28 85 46 13 BELGIUM Tel 32 24 82 30 30 GERMANY Tel 49 61 84 90 69 40 49 61 03 40 81 012 SPAIN or PORTUGAL Tel 34 9 32 23 31 54 JAPAN Tel 81 454 53 92 20 OTHER ASIA PACIFIC COUNTRIES FINLAND Tel 35 89 32 91 00 ITALY Tel 39 02 95 05 91 SWITZERLAND Tel 41 1 4 54 12 12 UNITED KINGDOM Other EUROPE RUSSIA CIS IRELAND MIDDLE EAST Tel 7 09 52 25 11 15 Tel 44 87 06 09 92 03 AFRICA Tel 49 61 84 90 69 40 33 2 28 03 20 00 UNITED STATES of AMERICA Tel 1 800 522 77 46 1 866 984 37 66 CANADA or LATIN AMERICA Tel 1 800 522 77 46 1 866 984 37 66 anderen L ndern Wenden Sie sich an Ihren rtlichen SORVALLe vertreter In Other Countries Contact your local SORVALLe products distributor agent Dans les autres pays Contactez votre concessionnaire local des produits SORVALL ou leur representant E mail Technical Service Representative for SORVALLe brand products at techsupport led asheville thermofishe
61. ntium processor running at 75 MHz or faster with an available serial communications port The computer must have at least 100MB of free hard drive space and be running Microsoft Windows V3 1 or 3 11 with at least 8 RAM Windows 95 with at least 16MB RAM or Windows NT V4 0 with at least 16MB RAM The computer requires a monitor keyboard mouse and printer with Windows compatible driver NOTE A dedicated directly connected computer is required WatchLog Network software will not operate correctly as a network server based application Also running other software applications accessories or utilities while WatchLog Network is in use is not advised The 2 Centrifuge is compatible with WatchLog Network M systems revisions below 2 0 require a software upgrade but is not compatible with older WatchLog software RC12BP Installation Chapter 2 INSTALLATION This chapter contains instructions to prepare the SORVALL RC 2BP centrifuge for operation Inspection 1 When youreceive your centrifuge carefully inspect it for any signs of shipping damage If you find damage report it immediately to the transportation company and file a damage claim then notify Thermo 2 Check the parts received against the shipping list if any parts are missing contact Thermo see back cover 3 Remove all packing material and remove any remaining items from inside the rotor chamber where this manual
62. ntually components will equilibrate and control will be appropriate NOTE When temperature control is critical the sample SET temperature rotor body buckets adapters and cover and rotor chamber should all be at the same tempera ture when START is pressed 4 12 HO T2BP M Operation To equilibrate rotor temperature before operation either store the rotor in a controlled temperature environment such as refrigerator or cold room or precool preheat the rotor in the centrifuge chamber until the rotor is the same temperature as the sample and the required SET temperature Temperature control needs can vary with the application In many cases the RC 2BP M Centrifuge will be used to run large volumes of sample for short amounts of time Over the course of a short run if all components have been equilibrated it would be difficult to signifi cantly change the sample temperature even if the centrifuge s temperature control performance was reduced Rotor Temperature Equilibration To equilibrate the rotor temperature in the centrifuge chamber 1 Install the rotor in the rotor chamber place empty buckets in all positions and install the rotor cover 2 Close the chamber door 3 Turn off all options access with MENU key 4 Future use if rotors are added Check to be sure that the correct rotor name is in the ROTOR field of the SET display if not press ROTOR until it is 5 Press SPEED If you are precooling
63. o air vents are blocked allowing for its physical size see figure 2 2 plus an additional 15 cm 6 inches on each side and a minimum of 5 cm 2 inches behind the centrifuge 10 cm 4 inches or more is desired Ambient air temperature at the centrifuge air inlets must be between 5 C to 40 C 41 F to 104 F with relative humidity lt 90 non condensing for the centrifuge to operate Ideal ambient temperature to meet all performance specifications is between 20 C to 35 C 68 F to 95 F therefore avoid areas near heat sources for example heating pipes and radiators Also avoid close grouping of centri fuges or other heat producing laboratory equipment Generally the cooler the location the better the operating conditions will be for the centrifuge 2 2 RC12BP Installation POWER CORD 244 cm 96 inch 112 8 cm 44 4 inch 106 7 cm 42 inch 83 8 cm 33 0 inch Figure 2 2 Centrifuge Dimensions Installation A Preinstallation Kit Catalog No 22001 is supplied for UL CE compliance Install the Preinstallation Kit according to instructions supplied with the kit install the RC 2BPTM 1 Position the RC 2BP in an operating location that satisfies the criteria specified in the previous Location paragraph With the Preinstallation Kit installed the operating location is so that the two tabs under the front edge of the centrifuge are flush against and align
64. ol mode TIME shows the MIN SEC value at termination the SET display shows the last TIME input value HOLD shows the MIN SEC value after deceleration to zero After an ACE controlled run ACE shows the total ACE value after deceleration to 0 the SET display shows the termination value If the previous run was not controlled by ACE pressing ACE shows the ACE value at termination the SET display shows the last ACE input value e The run TEMPERATURE C field indicates the current calculated sample temperature in degrees Celsius based on rotor selected rotor speed run time set temperature and measured temperature the calculation assumes that the sample temperature rotor temperature and SET temperature are all equal at the start of the run SET Display The SET display is a 2 line LCD array located at the top of the control panel The SET display is used to input and display basic run parameters to select options or other advanced features and to display advisory messages The display s contents are referred to in this manual as a screen for example the HOME screen described below displays basic run parameters that correspond to the Primary Function keys below the display e The HOME screen pictured above displays currently selected values for the basic run parameters ROTOR SPEED RCF TIMED HOLD ACE DEG C and if displaying a recalled program PROG The HOME screen appears in the SET display a
65. on status If an indicator is lit the option is selected MENU Accesses the advanced feature screens for selection of options and programming OPTION ac SLOW mm START RUN 04 00 22 01 SLOW STOP Starts centrifuge run Indicator is lit when a run is in progress STOP Stops centrifuge run Indicator is lit during deceleration Controls Indicators and Displays SORVALL Centrifuges RUN Display SPEED TIME TEMPERATURE RPM MIN SEC C Lior aly 2121 ILIL I RCF LL The RUN display is to the left of the control panel and is comprised of three fields of large red LEDs that indicate the current measured calculated conditions during a run e The run SPEED RPM RCF field indicates either the current measured rotor speed in rpm or if RCF is selected RCF indicator lit the relative centrifugal force or g force that is currently being generated at the maximum radius of the selected rotor The rpm values are rounded to 10s above 200 rpm At run completion zero speed after the door has been unlocked the display will show the word End until the door is opened e The run TIME MIN SEC ACE field indicates If controlled by TIME the display counts down from set time showing the time remaining in minutes and seconds until the run terminates and deceleration begins Ifcontrolled by HOLD indicated in the control panel SET display the display during a
66. ontami nation Information Certificate attached to it and in our opinion presents a potential radioactive or biological hazard our represen tative will not service the equipment until proper decontamination and certification has been completed If an instrument is received at our Service facilities and in our opinion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Disposition costs will be borne by the sender Decontamination Information Certificates are included with these Operation Instructions Additional certificates are available from your local technical or customer service representative In the event these certificates are not available a written statement certi fying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable NOTE Thermo Service representatives will indicate on a Customer Service Repair Report if decontamination was required and if so what the contaminate was and what procedure was used If no decontamination was required it should be so stated 01 06 INSTRUCTIONS When an instrument that has been used with radioactive patho genic or otherwise hazardous materials requires servicing by Thermo personnel either at the customer s laboratory or at Thermo facilities the following procedure must be complied with to insure safety of our personnel 1 The instrument or par
67. or the rotor the reduced speed can be determined by using the following formula Meduced Maximum Maximum Compartment Mass Speed Rotor Speed X bon eo dic rpm rpm Actual Compartment Mass 4 14 RC12BP Maintenance Chapter 5 MAINTENANCE This section describes routine maintenance procedures that should be performed on a regular basis As the user itis your responsibility to make certain that these activities are followed when necessary To keep your centrifuge in good working condition and ensure accurate test results we recommend that you have the centrifuge serviced annually by a Thermo Service Representative or other Thermo qualified service personnel If further service is needed contact Thermo Service or your local representative for SORVALL products WARNING There are no user service able items inside the centrifuge Be cause of the high voltages in the cen trifuge anyone who is not properly trained in electronics must not test or repair the electrical circuits WARNING If hazardous materials have been processed in the centrifuge ob Serve all necessary precautions when cleaning or servicing the centrifuge to avoid contamination N CAUTION Before using any cleaning or decontamination methods other than those recommended by Thermo users should check with Thermo or the local representative of SORVALL9 products that the proposed method will not damage the equipment MAINTENAN
68. perform the procedures listed under Speed Control Timer and Temperature Control NOTE f the centrifuge is to be connected with the optional Network Computer Interface Package for automatic qual ity control and run logging capability Thermo or a local representative for SORVALL products will install the package components as described in the WatchLog Network User s Manual supplied in the package After the 2 satisfies the inspection performance criteria it can be considered ready for use HO T2BP M Controls Indicators and Displays Chapter 3 CONTROLS DISPLAYS and INDICATORS The RCJ2BP controls displays and indicators have been designed for ease of use the control panel figure 3 1 allows entry with visual verification of set parameters and current conditions prompts action if an entry is inappropriate remains blinking and communicates status with user messages An optional Computer Interface Package allows for automatic on line logging of quality control and daily run data SPEED e TEMPERATURE zy 0 EE prz IE o Le OPTION INDICATORS Lights show advanced feature RUN DISPLAY SPEED RPM TEMPERATURE Displays rotor speed rpm Displays the current calcu gforce duringarun lated sample temperature when RCF indicator is lit C during a run TIME MIN SEC ACE During a TIME run displays remain
69. presentative will note on the Customer Service Repair Report if decontamination was required and if so what the contaminant was and what proce dure was used If no decontamination was required it will be so stated 5 7 5 8 RC12BP Appendix APPENDIX Appendix SORVALL Centrifuges Warranty Thermo Scientific makes no warranty of any kind expressed or implied except as stated in this warranty policy The SORVALL RC 2BP Centrifuge is warranted subject to the conditions specified below and in the warranty clause of the Thermo terms and conditions of sale in effect at the time of sale to be free from defects in material and workmanship for a period of one 1 year from the date of delivery Thermo will repair or replace and return free of charge any part which is returned to its factory within said period transportation prepaid by user and which is found upon inspection to have been defective in materials or workmanship This warranty does not include normal wear from use it does not apply to any instrument or part which has been altered by anyone other than an employee of Thermo nor to any instrument which has been damaged through accident negligence failure to follow operating instructions the use of electric currents or circuits other than those specified on the plate affixed to the instrument misuse or abuse Thermo reserves the right to change alter modify or improve any of its instruments wit
70. r com Visit our web site at http www sorvall com or http www thermo com SORVALL Thermo SCIENTIFIC
71. rk software so that centrifuge performance with respect to the SET speed and temperature is documented and can be verified NOTE if you plan on using the QC RUN feature consider the following as you save runs to program memory QC RUN programs should not use HOLD Programs with factory default settings of SPEED 400 rpm 00 00 and 40 will be ignored in the QC RUN sequence e Although you may choose otherwise to minimize the time it takes to perform a QC RUN we recommend the following Starting with program 1 save runs to program memory in order sorted first by temperature highest first then if there is more than one run at a single temperature by speed lowest first FOR EXAMPLE The programs at right are listed in correct order for using QC RUN PROG 5 with factory default settings will be ignored by the centrifuge when performing the run program values shown are for example purposes only actual set parameters will vary To select the QC RUN feature press MENU input 1 install the correct rotor make sure WatchLog Network M is running on the connected computer and press START The QC RUN sequence will begin running program number 1 and the run status will be displayed on the WatchLog Network com puter screen RC12BP Operation If during a QC RUN the observed RUN temperature is more than 2 C over SET temperature the DEG C field in the SET display will change to
72. rowsing through existing programs for selection or reference NOTE if you plan to use the QC RUN feature specific guidelines should be considered when saving parameters to memory see QC RUN on page 3 8 3 11 Controls Indicators and Displays SORVALL Centrifuges Numeric Keypad a e The Numeric Keypad is used to input speed time and temperature values into the HOME screen and to select the advanced features The ENTER key enters newly inputted speed time temperature and RCF values into memory it also allows you to bypass options in the OPTIONS sequence without selecting or deselecting them The CLEAR key sets a blinking value to zero in the SET display clears entry errors or fault messages and if the barcoding accessory is used and the door is open deletes barcode data input after the last run was completed keypad lock must be set to FULL FUNCTION The key is used to toggle between positive and negative temperature values 3 12 HO T2BP M Controls Indicators and Displays START and STOP Keys START STOP The green START key is used to start the centrifuge run When the START key is pressed the green LED status indicator on the key will blink until the rotor begins to spin which could take up to 20 seconds if SLOW START is selected Once the rotor starts spinning the light on the key will remain ON through run termination during deceleration until the
73. run counts up showing the time elapsed expressed in minutes and seconds up to a maximum of 300 00 since START was pressed or controlled by ACE Accumulated Centrifugal Effect ACE indicator lit the RUN display shows the current calculated ACE value When the set ACE value is reached the run terminates and decelerates to a stop The displayed value continues to calculate the centrifugal effect during deceleration to zero to show total accumulation Monitoring run duration in alternative terms During arun if an alternative run duration key is pressed once for example pressing TIME during an ACE run the RUN display will change to show an accumulating value in terms of the newly selected method The SET display and ACE indicator will not change but will continue to show the control method and value that was in effect when START was pressed Run duration will be controlled by the SET display value regardless of the alternative value showing in the RUN display If that same alternative key is pressed a second time the SET display will change to show a flashing value from the previous run expressed in that control method entry will change duration control to that method and the RUN display will continue counting based on the new entry Pressing START resets the run 3 2 RC12BP Controls Indicators and Displays Between runs the RUN display shows the previous run duration value viewable in each contr
74. rvice technician recalibrate the controls Speed Controls 1 Prepare a sample of test fluid Load and balance the rotor according to the instructions in the rotor manual 2 Install the rotor in the chamber and close the chamber door 3 If the RCF or SLOW START indicators are lit press MENU then press ENTER until the correct selection screen appears Press 0 to deselect each option indicator will turn OFF then press MENU to return to the HOME screen 4 Set the run parameters for a commonly used protocol with SPEED controlled by rpm and run duration set to HOLD 5 Press START wait for the SPEED value in the RUN display to reach the specified SET speed then wait an additional 5 minutes for speed to stabilize 6 Using an optical tachometer check rotor speed through the viewing port in the center of the chamber door in rpm following instructions supplied with the tachometer The tachometer should indicate rpm equal to the SET value 20 rpm or 1 whichever is greater 2000 rpm and below 20 rpm above 2000 1 If necessary repeat this procedure at other speeds that are commonly used in your protocols Timer Controls 1 Prepare a sample of test fluid Load and balance the rotor according to the instructions in the rotor manual 2 Install the rotor in the chamber and close the chamber door 3 Settherun parameters fora commonly used protocol but set the run duration by TIME MIN SEC to 13 30 5 3 Main
75. s not activated and a preheat mode begins if the RUN display temperature is more than 2 C under the SET value when START is pressed or when a temperature change between programs occurs in a STEP RUN In each case a SAMPLE UNDERTEMP PRE HEATING message will appear and an alarm will sound Pressing CLEAR removes the message and shuts offthe alarm allowing the run to continue as a preheat run P HEAT replaces DEG C asthe field headerin the SET display during preheat mode A preheat mode is automatic during a QC RUN the message and alarm are disabled and the program is repeated until temperature is within 2 of set e STEP RUN allows the linking together of up to three sets of programmed run parameters to automatically perform step run protocols When step run is selected the specified programs must specify the same rotor at similar temperatures Step runs can be saved to program memory program numbers 10 15 for simple recall in future use see SAVE RUN below e SAVERUN allows pre programming of up to 15 different sets of run parameters 9 standard runs and 6 step runs for simple recall anderror free run reproducibility In addition to basic parameters all option selections and settings excluding rotor name overtemperature limit and undertemperature limit will also be saved to program memory To eliminate inadvertent loss of existing programs the save run sequence alerts users before overwriting The convenient RECALL key allows b
76. should be so stated 01 06 INSTRUCTIONS When an instrument that has been used with radioactive patho genic or otherwise hazardous materials requires servicing by Thermo personnel either at the customer s laboratory or at Thermo facilities the following procedure must be complied with to insure safety of our personnel 1 The instrument or part to be serviced shall be cleaned of all blood and other encrusted material and decontaminated prior to servicing by our representative No radioactivity shall be detectable by survey equipment 2 ADecontamination Information Certificate shall be completed and attached to the instrument or part If an instrument or part to be serviced does not have a Decontami nation Information Certificate attached to it and in our opinion presents a potential radioactive or biological hazard our represen tative will not service the equipment until proper decontamination and certification has been completed If an instrument is received at our Service facilities and in our opinion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Disposition costs will be borne by the sender Decontamination Information Certificates are included with these Operation Instructions Additional certificates are available from your local technical or customer service representative In the event these certificates are not available a written
77. splay until the RUN temperature comes into range or until the run terminates After selection SELECT STEP RUN will appear in the display This screen allows you to link together up to three sets of run parameters from program memory to create a step run If the desired parameters have not been entered into memory you must save each desired set of parameters to memory before continuing with step run creation To create a step run input a program number 1 9 for the first portion of the step run The SET screen will prompt for another number input a program number 1 9 for the second portion of the step run The SET screen will prompt for another number and youcan either press ENTER if you are creating a two stage step run or input a program number 1 9 for the third portion of your run NOTE future use if new rotors have been added when step run is selected the specified programs should all use the same rotor 4 11 Operation SORVALL Centrifuges So that runs are not inadvertently corrupted whenever STEP RUN has been selected indicator lit changes to run parameters will be ignored unless STEP RUN is deselected before START is pressed Because of this the only changes that could be made without deselecting would be if a different step run was recalled from program memory program numbers 10 15 After STEP RUN selection SAVE TO PROGRAM MEMORY will appear in the display For future use step runs may be save
78. statement certi fying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable NOTE Thermo Service representatives will indicate on a Customer Service Repair Report if decontamination was required and if so what the contaminate was and what procedure was used If no decontamination was required it should be so stated 01 06 Great Britain Deutschland 159 Italia 159 France 159 WEEE Compliance WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 It is marked with the following symbol Thermo Scientific has contracted with one or more recycling disposal companies in each EU Member State and this product should be disposed of or recycled through them Further information on Thermo Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detec tion of substances subject to the RoHS Directive are available at www thermo com WEEERoHS WEEE Konformittat Dieses Produkt muss die EU Waste Electrical amp Electronic Equipment WEEE Richtlinie 2002 96EC erfiillen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Scientific hat Vereinbarungen getroffen mit Verwertungs Entsorgungsanlagen in allen EU Mitgliederstaaten und dieses Produkt muss durch diese Firmen w
79. t to be serviced shall be cleaned of all blood and other encrusted material and decontaminated prior to servicing by our representative No radioactivity shall be detectable by survey equipment 2 ADecontamination Information Certificate shall be completed and attached to the instrument or part If an instrument or part to be serviced does not have a Decontami nation Information Certificate attached to it and in our opinion presents a potential radioactive or biological hazard our represen tative will not service the equipment until proper decontamination and certification has been completed If an instrument is received at our Service facilities and in our opinion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Disposition costs will be borne by the sender Decontamination Information Certificates are included with these Operation Instructions Additional certificates are available from your local technical or customer service representative In the event these certificates are not available a written statement certi fying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable NOTE Thermo Service representatives will indicate on a Customer Service Repair Report if decontamination was required and if so what the contaminate was and what procedure was used If no decontamination was required it
80. te if the chamber is warm if so the rotor may be too hot to touch Unplug the centrifuge and contact Thermo Service WatchLog Network record FLOOR SENSOR The microprocessor has detected that the feedback current returning from the drive motor was improbable based on the current sent to the motor The fault indicates a motor drive box power supply or tachometer problem Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARNING on page 3 18 WatchLog Network record DRIVE 3 20 RC12BP Controls Indicators and Displays switch or that the motor temp circuit is otherwise open Wait for the rotor to stop spinning remove all sample from the rotor unplug the centrifuge and contact Thermo Service WatchLog Network record MOTOR SENSOR Speed was more than 200 rpm higher than the rotor s maximum rated speed indicating improper speed control or a faulty tachometer circuit Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARNING on page 3 18 WatchLog Network record ROTOR OVERSPEED Indicates that high brake resistor temperature has tripped its thermal switch indicating that the resistor was ener
81. tectable by survey equipment The SORVALL Product Guide contains descriptions of com monly used decontamination methods and a chart showing method compatibility with various materials The centrifuge or rotor instruction manual contains specific guidance about clean ing and decontamination methods appropriate for the product it describes Clean and decontaminate your centrifuge or rotor as follows For the centrifuge a Remove rotor from the rotor chamber b Remove wash and decontaminate motor sealing gas ket and pad c Decontaminate lid rotor chamber and drive using an appropriate method d Remove all encrusted material from around the motor and drive assemblies RC12BP Maintenance For rotors Remove tubes bottles and adapters from the rotor and decontaminate rotor using an appropriate method If tubes or rotor caps are stuck in the rotor or the rotor lid is stuck notify Thermo representative be prepared with the name and nature of the sample so the Thermo Chemical Hazards Officer can decide whether to authorize the rotor s return to a Thermo facility 2 Complete and attach Decontamination Information Certificate in the back of your rotor or instrument manual to the centrifuge or rotor before servicing or return to Thermo facility If Certifi cate is not available attach a written statement verifying decon tamination what was contaminant and what decontamination method was used If the centrifug
82. tely control sample temperature If a rotor is able to use buckets carriers with different maximum radii each configura tion will be listed for selection to calculate the different RCF The ROTOR key is not active when a run is in progress NOTE the future only after new rotors have been added ensure that the installed rotor is correctly identified by changing the SET rotor name immediately after changing the installed rotor SPEED is pressed to specify a different rotor speed in revolutions per minute rpm or relative centrifugal force RCF also known as g force than the value shown in the SET display Speed is selectable from 150 to 4700 rpm the maximum rated speed for the H 12000 rotor or as an equivalent RCF calculated at the maximum radius of the rotor rotor geometry only Changing from rpm to RCF or RCF to rpm is done by repeatedly pressing SPEED pressing once causes the value to flash so a change can be made in the existing mode pressing again changes to the other mode and displays a value equivalentto the previous pressing again causes that value to flash so a change can be made and so on An entered value will continue to flash prompting input when it is out of range for the rotor The formula used to calculate RCF is 2 RCF 11 17 x radius in cm x 1000 Controls Indicators and Displays SORVALL Centrifuges NOTE When changing mode rpm RCF values may appear a digit or two off from the original s
83. tenance SORVALL Centrifuges 4 Press START Using a stopwatch begin timing precisely as the RUN TIME display counts down to 13 20 then stop timing precisely as the RUN TIME display counts down to 00 00 The stopwatch should read between 13 15 to 13 25 representing 13 20 0 5 4 seconds plus an additional second to allow for cumulative human error If necessary repeat this procedure at other time ranges that are commonly used in your protocols Temperature Controls When verifying temperature control all test run conditions TEMP SPEED TIME ROTOR and option selections should match your required protocol s Before the run it is important for the test fluid to be at the same volume and temperature as the actual sample and for the SET temperature the displayed RUN Temperature and the temperature of all parts of the rotor assembly to match also ideally all rotor components would have been stored in the same controlled temperature area overnight but if that is not possible you can precool or preheat the rotor as required 1 Choosing a commonly used protocol with the longest run time set the centrifuge run parameters 2 Equilibrate the rotor chamber and the temperature of all rotor parts include any tubes bottles or adapters to be used to the desired sample temperature 3 Make sure the rotor chamber is wiped dry then install the rotor in the chamber and keep the chamber door closed until you load the rotor
84. the SET speed is too high for the newly specified rotor or if you recall a program that was saved based on the use of a different higher speed rotor Make sure the SET rotor specified is the installed rotor then enter an rpm or RCF value that does not exceed the maxi mum allowable for the rotor or install and specify a different rotor and press START 896 Future use if rotors are added This can occur when RUN or STEP RUN is in progress and a program is encoun tered that has a higher set speed than the maximum speed of the SET rotor indicating that the program was saved based on the use of a different higher speed rotor The SET rotor cannot be used with the programs as saved Make sure the SET rotor is installed change to an acceptable rotor or review and change the programs saved before reattempting WatchLog Network record SET SPEED START was pressed with a set run duration value of zero The centrifuge will not start Press one of the three run duration control keys TIME HOLD ACE input a value then press START 3 17 Controls Indicators and Displays WARNING Do not open the chamber door when the rotor is spinning In the event of a tachometer failure or any failure where RUN speed does not register the brake will not operate and the rotor will coast to a stop from high speed deceleration to O could take as long as 30 minutes Before using the mechanica
85. un duration to account for differences in acceleration thereby improving separation consistency and run reproducibility run after run from centrifuge to centrifuge e Rotor SPEED controlled by RPM revolutions per minute or by the RCF relative centrifugal force or g force e SLOW START chooses gentle acceleration from 0 to 250 rpm at 250 rpm acceleration transitions to the normal maximum rate with the slow start rate defined by the selection of one of ten different acceleration profiles e SLOW STOP chooses gentle deceleration from 500 to 0 rpm normal braking from set speed transitions to a more gradual rate at 500 rpm with the rate defined by selection of one of ten different braking profiles 1 3 Introduction amp Description SORVALL Centrifuges NOTE Selecting BRAKE OFF will affect SLOW STOP e BRAKE OFF deactivates normal braking for a coasting stop from a user specified transition speed e Automatic sample temperature calculation considers the run speed run time set temperature and measured chamber tempera ture to estimate and maintain sample temperature e Automatic self test performed by the microcomputer each time the centrifuge power is turned on to ensure proper performance e Automatic diagnostics so that if a system malfunction occurs message appears in the SET display to alert you of the condition Centrifuge Specifications Speed Maximum Selectable Speed 4700 rpm Maximum Relati
86. ur Always be aware of this possibility and take normal pre cautions Use appropriate decon tamination procedures should exposure occur 3 Locate the interlock motor then find the motor shaft override knob extending down from the bottom of the motor casing it is covered with a plastic sleeve Spin the knob in either direction with your finger until the door interlock bar has been drawn back far enough to disengage the door latch brackets so that the door can be opened using the latch handle 4 Reinstall the bezel in the front cabinet panel then plug in the power cord Parts Ordering Information To order replacement parts telephone toll free 1 800 522 7746 in the United States Outside the United States contact your local distribu tor or agent for SORVALL products Be sure to provide a description of the part plus the centrifuge model and serial number Service Decontamination Policy If a centrifuge or rotor that has been used with radioactive or pathogenic material requires servicing by Thermo personnel either at the customer s laboratory or at a Thermo facility comply with the following procedure to ensure the safety of all personnel 1 Clean the centrifuge or rotor to be serviced of all encrusted material and decontaminate it see Maintenance Section of centrifuge or rotor instruction manual prior to servicing by the Thermo representative or returning it to the Thermo facility There must be no radioactivity de
87. ure Remove all sample from the rotor after it stops then unplug the centrifuge and contact Thermo Service WatchLog Network record DOOR A run was in progress when the door lock switch opened indicating a circuit failure Remove all sample from the rotor after it stops then unplug the centrifuge and contact Thermo Service WatchLog Network record DOOR At run completion the door lock switch did not open indi cating a circuit failure The interlock motor is de energized another run will not start Remove all sample from the rotor then unplug the centrifuge and contact Thermo Service After START was pressed the door lock switch did not close indicating a circuit failure The run will not start and the inter lock motor is de energized Remove all sample from the rotor unplug the centrifuge and contact Thermo Service The microcomputer detected that the drive box was not responding possibly indicating an error internal to the drive box electronics or a brake resistor overheat condition Wait for the rotor to stop spinning this could take as long as 30 minutes then use the mechanical override to remove all sample from the rotor unplug the centrifuge and contact Thermo Service Read the WARNING on page 3 18 WatchLog Network record DRIVE 3 19 Controls Indicators and Displays SORVALL Centrifuges the tachometer was not responding as power was applied to the drive motor without any in
88. ve Centrifugal Force 7333 g Speed Control Accuracy 1 or 20 rpm whichever is greater Time Maximum Selectable Time 99 minutes 99 seconds or HOLD for indefinite length of time timed up to 300 00 Maximum Selectable ACE Accumulated Centrifugal Effect 9 99 x 10 Jordt input as 9 99e30 Temperature Temperature Selection Range 10 to 40 C Temperature Control Accuracy sample temperature controlled within 2 C of set temperature Overtemperature Alert Maximum allowable temperature settable to within 2 C of set temp Maximum Noise Level lt 65 Maximum Average Heat Output 5 3 kW With sample rotor and chamber temperatures equal to SET temperature at the start of the run or during a run after those components reach equilibrium Control range is reducedif run speed runtime settemperature ambienttemperature variables combine to create an extreme condition beyond the temperature control system capacity Measured 3 feet from the front panel at a height of four feet using an H 12000 rotor at its top speed of 4700 rpm 1 4 RC12BP Introduction amp Description Weight uncrated without rotor Dimensions Width Depth Height top of control console Length power cord minimum Other Depth back of door to back panel Height top of open door Height rotor installation lift over Diameter chamber opening Supply Power Requirements Supply Configurations AC Recommended Supply Current Single
89. zardous materials and do not work on top of or next to the centrifuge Do not attempt to open the chamber door when the rotor is spinning never override or otherwise disable any of the safety systems of the centrifuge CAUTION Do notrun or precool preheat a rotor at the critical speed as this will have a detrimental effect on centrifuge component life See Chapter 4 Rotor Temperature Equilibration page 4 13 Do not operate the centrifuge with a rotor out of balance To do so can cause damage to the centrifuge drive assembly Do not operate centrifuge without a rotor properly installed and locked to the drive and the rotor cover if any must be properly installed See rotor instruction manual Locate the centrifuge on a level floor to avoid rotor imbalance during operation The centrifuge can be damaged if connected to the wrong voltage Check the voltage before plugging the centrifuge into a power source Thermo is not responsible for incorrect installation Always maintain the centrifuge in the recommended manner See Chapter 5 Maintenance iii Table of Contents iv Table of Contents Safety Information Page SORVALL Centrifuges Page ill Chapter 1 INTRODUCTION amp DESCRIPTION Centrifuge Description Centrifuge Specifications ixi eee Parts Suppliet Optional Computer Interface Package Chapter 2 INSTALLATION METRE Tm Electrical Requir

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