SensorTrace Photo Manual
Contents
1. algae mat coral Photo software The microsensor is connected via the LEMO connector to a pA channel on the front of the meter while the UNISENSE Light Sensor box is connected to an analogue input channel at the rear The UNISENSE Microsensor Mono or Multimeter is connected to a USB port of the computer The microsensor is placed in a micromanipulator that is used to position the sensor tip at the intended measuring site of the studied sample The tip of the light sensor is positioned i e with a lab stand within the light field but placed so that it is not interfering with the path of light towards the measuring site Thus the light sensor signal acts as an exact indicator for the time of light to dark transition 15 INSTALLING THE SOFTWARE Make sure that you are installing having the full administration rights Start the installation program exe file from the CD Follow the instructions given by the installation program This will install the SensorTrace Suite program including a version of this manual in the program group Unisense Instrument drivers and a configuration utility for the A D converter will also be installed This is called Instacal and will be placed in a program group called Measurement Computing To activate the full SensorTrace Suite program and gain access to Logger Profiling Photo and Rate enter the License Key supplied with the installation CD To access the dialog box for entering2. Unit Select an appropriate concentration unit for the sensor signal when calibrated 20 Range V Select the voltage range for the A D converter Select the smallest range possible to get the most out of the resolution of the A D converter matching the expected signal range of the sensors It is recommended not to select an unnecessary high range as this may cause a loss in resolution However the range should not be chosen so small that the signal gets beyond the selected range This will cause the A D converter to get saturated Name Write a name describing your sensor optional Comment Write a comment about your sensor optional Motors The number of connected motors detected by the program are shown at the bottom of the window If a 3 D motorized micro manipulator system is used motors Z X and Y should be recognized For a1 D stage only motor stage Z will be identified For each motor unit it is possible to adjust the settings for motor velocity and acceleration before the experiment is started If a manual micromanipulator is part ofthe experiment setup a manual motor can be included and the user will be prompted every time the sensor sould be moved between measurements After the sensor and motor settings have been checked and adjusted accordingly press the START EXPERIMENT button The software will subsequently make several tabs available The sensor and A D converter settings will be saved and more tabs will app
3. 630 411 28 05 2013 12 14 30 627 172 075 445 987 630 458 28 05 2013 12 14 30 829 171 901 445 624 630 505 28 05 2013 12 14 31 286 171 727 445 261 630 552 28 05 2013 12 14 31 500 171 553 444 898 630 599 28 05 2013 12 14 31 721 171 379 444 535 630 646 28 05 2013 12 14 31 925 171 205 630 693 8 05 2013 12 14 32 171 031 630 74 O O 0 0000 OO00 0 Please note that a is always used as a decimal delimiter EXPERIMENTAL DATA EXAMPLE This section gives an example of an experimental data set for photosynthesis rate measurements obtained using the light dark shift method The measurements were performed in a highly diverse microbial sediment community with high photosynthetic activity Light dark shift measurements in photosynthetic microbial mat In the exemplified study the photosynthetic rates were measured over a vertical micro gradient from 1000um above the sediment surface to a depth of 2000um Measurements were performed and at a spatial resolution of 100um A fast responding oxygen sensor with a tip diameter of 50um and a response time 0 5 seconds was used 40 Slope Steady state Slope Slopelcal s 0 0 049 Steady state 837 466 Ri 0 167 StDevical 0 209 21 807 Calibrated 2000 ms Example of rate measurement performed at 300um sample depth within the zone with very high photosynthetic activity In the presented study the photos
4. 7 32 bit 64 Windows 8 8 1 e min 200 MB free hard disk space e USBport s e min screen resolution 1280 x 800 min 4 GB RAM Other programs available in the full SensorTrace Suite SensorTrace Profiling For motor control activity calculations and profiling in 1 2 or 3 dimensions we recommend that you use SensorTrace Logger is the basic data acquisition program from the Unisense program SensorTrace Suite It offers timeseries datalogging and calibration features SensorTrace Rate For microrespiration experiments to measure the metabolic rates including respiration rates of small aguatic animals bacteria or oxygen production of phytoplankton we recommend that you use Bi LIGHT DARK SHIFT METHOD This paragraph gives a short description of the theory on which the light dark shift method is based The tip of an oxygen microsensor is placed at a certain position r in a photosynthetic system It measures at time t the oxygen concentration C r t The time dependency i e the derivative in time of the oxygen concentration can be described by Ot where Pis the volumetric gross photosynthesis rate of oxygen mol m 3 1 and R the volumetric oxygen respiration rate mol m3 5 1 respectively The volumetric transport rate T repre sents the changes in oxygen concentration due to transport phenomena mol m3 s 1 The type of transport is dependent on the specific sample under investigation It can be due to a
5. manually adjusted 30 4 Sensors and Motors Contains the elements for sen sor and motor control 31 MOTOR CONTROL The illustrated box is used for control of the motorized microma nipulator and hence it is used for exact positioning of the sensor tip during light dark experiments Actual Depth um 300 Go Home New Depth um Move by step um 100 TN The shown depth unit is always micrometers Actual Depth indicates the current depth position of the microsensor tip If you press GOTO the microsensor will be moved to a new depth as set under New Depth Under Move by step you can press the UP or DOWN keys will move the micro sensor one step up or down respectively as indicated by the set step size Further control options are the SET HOME tab which adjusts the current depth position of the sensor to zero and the GO HOME tab that will bring the sensor to an already defined zero depth position Access to the control of other connected motor stages is obtained by clicking the Motor X and Motor Y tabs respectively Identical control features are available for these motor stages as described for Motor Z 32 PERFORMING EXPERIMENT SETTINGS Before the actual measurement can be started some parameters have to be adjusted in the experimental window These were already described in the paragraph The Light Dark Shift Method Settings Samples s 20 Duration s Before swi
6. the license key click on the ENTER LICENSE KEY button in the low right corner for the main SensorTrace Suite window Press ACTIVATE when the key is fully entered Contact SALES UNISENSE COM to purchase a license key Enter license key Register product Enter the license key to active the product License key GETTING STARTED 1 Set the PC power management to ALWAYS And make sure that your PC does not enter sleep mode or stand by during measurements as this will inter rupt the connection to the instruments and it will be necessary to restart the program 2 Connect all instruments in your set up to the com puter 3 Start SensorTrace Suite it is placed in the program group Unisense The following main program window appears mn SensorTrace Suite UNISENSE Load Experiment Knowledge Base No 4 Choose either to make a new Photo experiment or load an old experiment a New Experiment When a new experiment is selected the user is asked to create a new experiment from the dialog box that appears Create the new experiment by naming experiment and researcher identitiy followed by an optional brief experimental description 17 18 Finally select an exsisting master experiment or create a new master experiment then press the CREATE button to create the new experiment The master experiment allows the user to group several sub experiments e g on the same sample
7. various parameters for the sensor s can be chosen The software will automatically start searching for connected instruments i e MultiMeters and motor controllers If no instru ments are recognized it is possible to manually repeat the scan In the settings window the registered sensor channels are found at the top For each sensor channel there are several setting options From left to right the user can adjust the following information The Settings tab Status of the sensor channel sensor type sensor measuring unit shown with all output range and sensor name Furthermore it is possible to add a menus down for an short comment for each sensor overview File Tools Windows Help Start Experiment Scan instruments and configure them Instrument Y Use Y Channel Y Type Y Unit Y Range Y SensorName Y Comment y ADC216 S N 4279 O pmol l MUI v O Sensorl ADC216 S N 4279 B Oz mg l 1 O Sensor 2 Multimeter S N 5016 m 1 25 pmol l 32 O Sensor 3 Multimeter S N 5016 2 He oma O Sensor 4 Multimeter S N 5016 3 meant ZW O Sensor 5 NO mmHg Multimeter S N 5016 4 5 O Sensor 6 pH a u Multimeter S N 5016 m 5 Redox mol l 35 O Sensor 7 Multimeter S N 5016 m 6 Temperature mol l 5 O Sensor 8 NO Sensor Mark the checkboxes for the channel sensors you want to view and record signals from Type Choose sensor type from the drop down menu ifthe default value is not appropriate
8. OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE UNISENSE SHALL NOT BE LIABLE FOR ANY DAMAGES OF ANY KIND INCLUDING INCIDENTAL SPECIAL PUNITIVE CONSEQUENTIAL AND SIMILAR DAMAGES INCLUDING WITHOUT LIMITATION LOSS OF PRODUCTION LOSS OF PROFIT LOSS OF DATA LOSS OF GOODWILL LOSS OF CONTRACTS OR BUSINESS INTERRUPTION This License Agreement and any dispute arising out of or in relation to this License Agreement shall be governed by and construed in accordance with the laws of Denmark exclusive of its choice of law provisions The venue for any such dispute shall be the Danish courts provided however that Unisense shall be entitled to instigate legal proceedings against the Customer before the courts with jurisdiction over the matter located in a country where the Customer has a place of business or is incorpo rated or organized OVERVIEW SensorTrace Photo For photosynthetic experiments using the light dark switch technique we recommend that you use SensorTrace Suite is compatible with all digital Unisense instru ments It supports up to 16 channels with an adjustable logging frequency of up to 50 samples per second depending on the Unisense amplifier connected The program automatically saves all data in an SOL database and all data can be exported in csv formatted files for subsequent data analysis SYSTEM REQUIREMENTS e A digital Unisense amplifier or A D converter unit e PC with Windows XP 2000 Vista Windows
9. OFILE WINDOWS 37 OUTPUT EIER een iio nana 38 EXPERIMENTAL DATA EXAMPLE 0 0 ccc ccc ccc NNN 40 CONGRATULATIONS WITH YOUR NEW PRODUCT SUPPORT ORDERING AND CONTACT INFORMATION If you wish to order additional products or if you encounter any problems and need scientific technical assistance please do not hesitate to contact our sales and support team We will respond to your inquiry within one working day E mail sales unisense com Unisense A S Tueager 1 DK 8200 Aarhus N Denmark Tel 45 8944 9500 Fax 45 8944 9549 Further documentation and support is available at our website www unisense com WARRANTY AND LIABILITY Unisense SensorTrace Suite software is checked and validated on the operating systems as given in the specification running English language settings It comes with lifetime updates Software must be installed under administrator rights Customer must ensure PC is fully updated and no conflicting third party software is installed Unisense do not warrant compliance with any other operating systems language settings or third party software For instrumentation and sensors please refer to our warranty conditions as given in the document General Terms of Sale and Delivery of Unisense A S found on www unisense com License agreement The following terms shall apply to the software provided by Unisense A S Unisense in connection with the simultaneous sale to you Customer of a Unis
10. UNISENSE UNISENSE SENSORTRACE SUITE SENSORTRACE PHOTO USER MANUAL SENSORT RACE SUITE V2 0 User MANUAL Copyright 2013 Unisense A S Version October 2013 SENSORTRACE PHOTO USER MANUAL UNISENSE 5 TABLE OF CONTENTS SENSORTRACE ma dead ne naeh 1 USER MANUAL ura e io a 1 CONGRATULATIONS WITH YOUR NEW PRODUCT 7 SUPPORT ORDERING AND CONTACT INFORMATION 7 WARRANTY AND LIABILITY 2 U rl 8 OVERVIE N s e dada cies es erent H EES eng 11 THE LIGHT DARK SHIFT METHOD u Ren N 12 MEASURING SET UP adedccutidainwedbetidics ses bah de onde neat S niva K ae UK 15 INSTALLING THE SOFTWARE 222 rer 16 G TARTEL 22 2 senses 17 SENSORTRACE PHOTO INTERFACE cere 19 SETTING VAR gx oer ia taa iaa 20 COMMENTS LA ia ro eo 22 CALIBR AMON WAG 022 ra anio ea druida iaa Aa 24 CALIBRATION PROCEDURE 25 Recalibration procedure 26 Retrieving a calibration 26 CALIBRATION OF LIGHT SENSOR 2 442432 850254 ae tern 27 TAHE LIVE DATA GRAPH dl dae ee 28 ESPERIMENT TAE TE a renta ras 30 MOTOR CONTROL 32 PERFORMING AN EXPERIMENT ws cu 33 SETTINGS 33 PHOTOSYNTHESIS RATE MEASUREMENTS 34 PR
11. dvection convection or diffusion The light dark shift method works for all types of transport For the light dark shift technique it is important that the oxygen concentration reaches a measurable steady state value under a constant illumination setting At steady state the above equation is equal to zero 5 gt T R P 0 Ot If now the illumination is switched off the steady state situation is disturbed The photosynthesis rate P is now zero whereas transport and respiration rates are assumed to remain unchanged within the next short time period Thus the time dependency of the oxygen concentration C immediately after the onset of darkness is given by T R Ot The latter two equations can be subtracted from each other which yield pt Ot Thus the volumetric gross photosynthesis rate P can be deter mined by the slope of the oxygen concentration C immediately after the light dark shift This is indicated in the following figure steady state light dark t Practically the measurements are realized by moving the oxygen microsensor tip to the measuring site with the help of a micromanipulator The sample of interest is illuminated with photosynthetically active light of a constant intensity Steady state is achieved when the microsensor reading is stable over time At this point the illumination is switched off It is essential that the switching event is fast lt lt 100 ms Hence just switching off the li
12. e Live Data The y axis scale can be modified by zooming like for the x axis Calibrated un calibrated The user can control whether the graphs show calibrated signals or raw signals for the sensors by checking the CALIBRATED checkbox for each graph If no calibration has been performed see CALIBRATION TAB section below and the checkbox is checked no signals will be plotted Chart legend At the top of the chart there is a sensor legend showing the graph colour for the associated sensors and their current signal concen tration value Datapoints The datapoints in the Live Data window can be cleared by pressing CLEAR This will NOT affect the stored data 29 EXPERIMENT TAB The main experimental window is used during photosynthesis rate measurements and is composed of a number of different elements J Calibrated 9 Steady state 2500 Ende 0000 EME Startjs 0 000 Endis 1500 The main display features are 1 SETTINGS In this window the experimental set tings are pre adjusted for the photosynthetic rate measurements 2 OVERVIEW This window contains two graphs that summarize the results of the performed ex periments by showing the measured steady state concentrations and slopes as a function of sample depth 3 MEASURING Displays the sensor signals during the last performed light dark measurement The values for steady state and slope are shown and the time interval for calculations can be
13. ear Calibration Note that settings cannot be changed after starting 21 COMMENTS TAB The COMMENTS TAB allows the user to enter notes and comments regarding the measurements in the file Any text that you want to save with your data e g a general description of your experiment can be entered W SensorTrace Logger Inisens erimen File Tools Windows Help Settings Calibration Data Logger ESTU i Sensors Time Y Type Y Comment y 06 05 2013 12 52 18 StartExperiment Logger demo experiment Current Experiment Use current time y O Sensor 1 152 60 mV IAE amp 2 O Sensor 3 0 46 mV amp Oz Sensor 7 3 54 mV O Sensor 8 2 65 mV l E Calibrated Show last 2000 00 Zen 12 57 10 12 57 20 12 57 30 12 57 40 12 57 50 12 58 00 Zoom Pause Picture may be common between ST Logger Photo Rate and Profiler SensorTrace Photo allows the user to make comments with a timestamp which will be displayed on both the Live Data graph and Logger graphs This enables the user to get a better overview of the course of an experiment Pressing ADD will add the comment along with a timestamp from the time the ADD button is pushed 22 The timestamp can be updated by entering the time manually Thus this feature allows the user to either 1 press the ADD button just when observing an unforeseen event and then write the appropriate text later or 2 write the t
14. ense SensorTrace Suite Software All rights title and interest in the software belong to Unisense Unisense grants to the Customer a royalty free non exclusive and non transferable license to use the software solely in connection with the Unisense Product purchased from Unisense simultane ously with the purchase of the software The Customer undertakes not to copy modify reverse engineer disassemble or de compile all or any part of the software or rent lease distribute or sell the software The Customer shall however be entitled to make one copy of the software for back up and recovery purposes for use solely in connection with the Unisense Products supplied by Unisense together with the software Nothing in this License Agreement or any other agreement between Unisense and the Customer shall be construed as an obligation for Unisense to provide to the Customer updates of the software This License Agreement shall automatically terminate if the Customer violates the terms of the license In case of termi nation of the license the Customer shall immediately destroy the software and any copy thereof CUSTOMER TAKES SOFTWARE AS IS UNISENSE MAKES NO WARRANTY OR REPRESENTATION CONCERNING THE SOFTWARE AND EXPRESSLY DISCLAIMS ALL OTHER WARRANTIES AND CONDITIONS EXPRESS OR IMPLIED STATUTORY OR OTHERWISE OF WHATEVER KIND OR NATURE INCLUDING BUT NOT LIMITED TO ANY AND ALL IMPLIED WARRANTIES INCLUDING IMPLIED WARRANTIES
15. ext first wait for an expected event and enter the time when the event occurs The events will be listed in the table to the right and an indication will be displayed in the graph in the Live Data window During logging an experiment you can also press the COMMENTS button and get the below comments dialog box Comment Use current time Y Enter dat O Logger comment In addition to the user generated comments the program will time stamp and show events for instance calibrations All comments and events are stored in the internal database file x 23 CALIBRATION TAB Calibrations are performed in the CALIBRATION TAB The individual sensor tabs at the top show whether the sensors are calibrated or not Uncalibrated XK O Sensor 1 Calibrated w 42 Sensor 1 i i Choose the sensor tab that you would like to calibrate The sensor name type and calibration unit is shown for each sensor The mV signal in the middle is the current raw sensor signal for the chosen sensor The sensor signal can also be followed continuously in the Live Data graph at the bottom of the tab 24 Kenn at Min CALIBRATION PROCEDURE 1 2 Prepare the calibration samples Choose the sensor you want to calibate Change the concentration in the concentration box according to the actual calibration solution For oxygen an automated procedure to calculate the atmospheric saturation as a function of tempera ture a
16. ght source will usually not work as most light sources exhibit an afterglow Normally sample shading will be the best method This can be done by either blocking the light path manually e g using a piece of cardboard or aluminium foil or by applying an electronic shutter 13 The computer samples the voltage readings of the microsensor at a frequency of 10 50 Hz The typical sampling interval starts 1 3 seconds before the switching event and stops 2 4 seconds after it The time interval before the switching event is used to calculate an average value of the steady state oxygen concentration The volumetric gross photosynthesis rate is obtained by fitting a linear slope to the data sampled immediately after darkening The user has to define the start and end time of the fitting interval relative to the switching event Typical values for the fitting interval are 0 0 5 and 0 5 2 seconds for the start and end times respectively As the Photo software has to know exactly when the switching took place a light sensor is placed within the light field near the measuring site Its signal is used by the computer as a trigger for the switching event MEASURING SETUP In order to realize ligh dark measurement the following compo nents are required for the experimental setup Fast responding O micro sensor Light sensor including amplifier box e Micromanipulator e Microsensor Monometer or Multimeter e Photosynthetic active sample i e
17. lt the sensor manuals RECALIBRATION PROCEDURE Sensors can be recalibrated at any time during an experiment The new calibration applies from the time of calibration onward To recalibrate the sensor 1 Press CLEAR ALL POINTS 2 Follow step 1 6 in the Calibration procedure All calibration data are stored in the SensorTrace Suite database RETRIEVING A CALIBRATION Pressing RETRIEVE CALIBRATION will retrieve the calibration of the sensor made the last time the program was running If you want to use the retrieved calibration remember to press APPLY CALIBRATION after retrieving a calibration Note When retrieving a calibration you are loading an older cali bration made previously Make sure that the retrieved calibration matches your current sensor in terms of signal size and units and that the calibration temperature etc are still valid 26 IMPORTANT When retrieving a calibration you will load the last calibration made the previous time the program was running Make sure that the retrieved calibration matches your current sensor in terms of signal size and units CALIBRATION OF LIGHT SENSOR During calibration of the light sensor it is recommended that the sensor is physically placed in the same position that is used for the actual measurements Press SET LIGHT ON during illumination Now switch the illumination off and press SET LIGHT OFF The actual voltage readings of the light sensor are shown in the boxes nex
18. n data includes the fitted lines in thick black for both steady state and slope intervals Both intervals can be manually adjusted following the measurement to optimize the curve fit The calculated values for steady state uM and slope uM O ms including statistical analysis of the data are shown in the box next to the graph These values will be automatically updated when the interval is adjusted The of the line fittings is a value between 0 and 1 and the closer the slopeline is to 1 the better the fit When a measurement has been completed the data is being saved to the file by clicking the SAVE button Alternatively if the signal data from the current measurement for some reason should not be saved i e if Photo did not detect the trigger signal or if the measured data was of poor quality the CLEAR button will erase the recorded data and the measurement can be repeated 35 After the measurement has been saved the sensor can now be moved from the current to the next position using the motor controls and a new light dark experiment can be performed Depending on the selected step size i e 100um this will generate a data set that at high spatial resolution describes the vertical distribution of the photosynthetic activity within the analyzed sample 36 PROFILE WINDOWS All measurements that are saved will be displayed in two separate graphs in the OVERVIEW window and these graphs will be updated grad
19. nd salinity can be invoked by pressing the button named CALCULATE O Conc The below dialog box will be shown Get O value CALCULATE OXYGEN AT AIR SATURATION Temperature C 20 00 Salinity o 0 00 Concentration 283 pmol l After entering the correct concentration add the calibration point by pressing ADD POINT Several points can be added for each concentration Change to another calibration standard and repeat points 3 4 It is possible to use several different standards and make a multi point calibration to verify linearity Ifa calibration point is not valid e g due to typing errors a single point can be cleared by selecting it with the mouse and pressing DELETE POINT All calibration points can be removed by pressing CLEAR ALL POINTS When you are satisfied with your calibration press APPLY CALIBRATION A linear regression will be per 25 formed based on the calibration and this regres sion will form the basis for converting signals to calibrated values Values are displayed in the table 8 Repeat 2 7 for other sensors The calibration table below the calibration graph shows the calibrations for the chosen sensor Each calibration will appear here with information on calibration number time of calibration linear regression data slope intercept and r as well as additional user comments that can be entered directly into the table For information on calibration of a specific sensors consu
20. or sample station New Experiment Settings Experiment Name Unisense experiment Researcher Name Unisense demo Experiment Description Logger demo experiment O Master Experiment 1 Master Experiment Create new Master experiment Load Experiment A dialog box appears were you can choose the experiment that you would like to open Press LOAD when the experiment is selected This mode is for working with old data settings and parameters cannot be changed and new measurements cannot be started SENSORTRACE PHOTO INTERFACE The SensorTrace Photo interface is divided into two main areas The upper area is divided into tabs which allow the user to access different functions of the program The lower area the Live DATA shows the sensor signals and event marks continuously to provide the user with an overview of the entire experiment The Live DATA is shown in all tabs of the program samperi x Steady State Sope ughe E o 3000 2000 1000 a 19 Reedy mare Hart 2500 trex 0000 PSE Su 0000 1360 In the right side area sensor selection and if available motor controls are found All components will be described in detail on the following pages 19 SETTINGS TAB After clicking the CREATE button in the experiment setup dialog box the first tab to appear in the upper area is the SETTTINGS TAB This tab will display the detected hardware and sensors In the Sensors table
21. ow figure an example data file has been loaded into Microsoft Excel ogger cs Microsoft Exce Insert Page Layout Formulas Data Review View Nitro Pro 8 Team B Calibri 2 General E Conditional Formatting gt 3 Insert v Er ay 3 B Fa Afa E Format as Table 3 Delete 3 gt Paste v Sort amp Find amp E A E ES E 5 Cell Styles BEY Format Zr Fier Selects Clipboard 3 m Number Time YYYY MM DD HH MM SS Time MS Sensor 1 mV Sensor 1 umol l Sensor 2 mV Sensor 2 pmol l 28 05 2013 12 14 26 273 175 207 452 521 629 612 28 05 2013 12 14 26 476 175 033 452 158 629 659 28 05 2013 12 14 26 683 174 859 451 795 629 706 28 05 2013 12 14 27 148 174 685 451 432 629 753 28 05 2013 12 14 27 414 174 511 451 069 629 8 28 05 2013 12 14 27 622 174 337 450 706 629 847 28 05 2013 12 14 27 824 174 163 450 343 629 894 28 05 2013 12 14 28 36 173 989 449 98 629 941 28 05 2013 12 14 28 322 173 815 449 617 629 988 28 05 2013 12 14 28 519 173 641 449 254 630 035 28 05 2013 12 14 28 753 173 467 448 891 630 082 28 05 2013 12 14 29 20 173 293 448 528 630 129 28 05 2013 12 14 29 269 173 119 448 165 630 176 28 05 2013 12 14 29 538 172 945 447 802 630 223 28 05 2013 12 14 29 753 172 771 447 439 630 27 28 05 2013 12 14 30 20 172 597 447 076 630 317 28 05 2013 12 14 30 230 172 423 446 713 630 364 28 05 2013 12 14 30 423 172 249 446 35
22. re may be common between SensorTrace Logger Photo Rate and Profiler Comments calibrations points and other events generated by the user or the program can be seen as colored marks in the Live Data window By holding the cursor over the mark the associated text is displayed next to the mark For further information on comments and events see also the section on the COMMENTS TAB 28 X axis scale By default the time scale x axis is controlled in the SHOW LAST drop down list where a number of preset time intervals can be chosen To have a look at a certain time span zoom in on this area by dragging a rectangle with the mouse from the upper left corner to the lower right corner of the area of interest The mouse wheel can also be used To un zoom rigth click and select ZOOM OUT or double click directly on the graph 0 Sensor 1 405 64 mV 2 O Sensor 2 4405 00 mV 61 O Sensor 3 0 40 mV Sensor 7 3 62 mV El O Sensor 8 2 68 mV _ Calibrated 0 0 9 Show last 2000 00 1min 7 ne es sivu 13 17 00 13 17 10 13 17 20 13 17 30 13 17 40 13 17 50 Zoom By clicking the PAUSE check box the auto updating is halted and zooming and scrollinng through the data is easier especially if you have selected a fast sampling interval Data is still logged and un checking the PAUSE will update the graph Y axis scale By default the y axis autoscales to accommodate the maximum and minimum signals that are shown in th
23. t to the buttons The calibration of the light sensor is now activated by pressing the apply calibration tab It is recommended to test that the light trigger will actually capture a change in light regime during light on off transitions by turning the light source repeatedly on and off a couple of times while observing the status of the sensor The software is pre programmed to respond to light threshold values that are somewhat lower higher that the recorded calibration values for light on and light off respectively It is further possible to adjust these readings i e the light on value downwards and the light off value upwards to further ensure that the light transition is captured during experiments The calibrated light signal is shown as RAW and the value repre sents the current signal in units of compared with the set signal value for light on 27 THE LIVE DATA GRAPH The Live Data graph is permanently visible in the lower part of the SensorTrace Suite interface It allows the user to view sensor signals continuously By default uncalibrated raw sensor signals are shown but ifthe CALIBRATED check box is checked calibrated values are plotted for calibrated sensors The Live Data holds a maximum of 1 million datapoints in total Thus as an example the Live Data graph can show 500 000 points for each of two sensors You can change the height ofthe window by dragging its upper border A CS KEN 00 Pictu
24. tch 2000 After switch 3 500 Slope interval Start s 0500 End s 1500 The sampling rate or sampling frequency is set in SAMPLES SEC The total sampling duration is set in BEFORE SWITCH and FTER SWITCH These parameters define the recorded time interval in seconds before the switching event and after it respectively The time interval used for the slope fitting is defined in START and END The values are given in seconds relative to the switching event Note that the fitting interval can be interactively changed for each experiment after the measurement has been made see below 33 PHOTOSYNTHESIS RATE MEASUREMENTS Now the actual measurement can be started The oxygen micro sensor tip is positioned at the measuring site of the illuminated sample The oxygen sensor reading in the Live data window has to be observed until the signal reaches a stable value which thus indicates that the system is in a steady state Now click the MEASURE tab On the screen the message Measuring will be shown which indicates that the measurement has started After a short time period as set in Before switch this message will change to Turn of the light signalling that it is time to darken the sample It is important to wait with the switch event for at least as many seconds as set under Before switch because the trigger signal of the light sensor can otherwise not be detected Keep the sample in the dark un
25. ter directly to your measuring set up with a wire going from the Ground connection of the sensor instrument to the liquid you are measuring in Ifyou encounter other problems and need scientific technical assistance please contact sales unisense com for online support we will answer you within one workday 42 E UNISENSE UNISENSE DENMARK www unisense com info unisense com
26. til the Measurement window is opened but not unnecessarily long as an extended darkening period will prolong the time period before a new steady state is reached and the next rate measurement can be made MS Simady da e Se 0 0000 Save Clear Eevee Suh 0508 Eadie 1408 7 34 The MEASUREMENT window displays two charts with recorded signal data for the light sensor and the oxygen sensor during the light dark transition The captured data are shown either as calibrated values or as raw signals The graph that shows the oxygen data includes the fitted lines in thick black for both steady state and slope intervals Both intervals can be manually adjusted following the measurement to optimize the curve fit The Measurement window displays two charts with recorded signal data for the light sensor and the oxygen sensor during the light dark transition The captured data are shown either as calibrated values or as raw signals The right hand graph that shows the oxygen data includes the fitted lines in thick black for both steady state and slope intervals Both intervals can be manually adjusted following the measurement to optimize the curve fit The Measurement window displays two charts with recorded signal data for the light sensor and the oxygen sensor during the light dark transition The captured data are shown either as cali brated values or as raw signals The right hand graph that shows the oxyge
27. ually as the experiment progresses The two graph plots show steady state values and calculated slopes respectively as a function of depth in the sample The y axis refers to the depth position um where the data points have been acquired The x axis refers either to the either the voltage value or to the calibrated value Steady State Slope The two plots give the user an overview of the measured distri bution of the photosynthetic activity within the studied sample From the combined data it is possible to clearly identify where in the sample that the photosynthetic activity is found 37 OUTPUT FILE All saved profiles and text entered and stored in your SensorTrace Photo program are logged to an internal SensorTrace Suite database However all data including raw data are accessible through data export in an Microsoft Excel compatible output file format CSV to facilitate processing and graphic representation of the data All you need is to make sure that you have a program that can view or import CSV files e g Microsoft Excel files Click the EXPORT button to save data E senor ace goer Te x Y ile Tools Windows 800 00 MotorX Motor Y Actual Depth um 0 se one New Depthlum o Move by stepium Calibrated 850 00 Show last 15 59 00 16 00 00 16 01 00 16 02 00 16 03 00 Oz Sensor 2 647 35 mV Calibrated Show last 800 00 5min Clear Zoom Pause 38 In the bel
28. ynthetic active zone spanned a layer about 1 mm wide from the sample surface The highest rate of photosynthesis was measured within a 200 400um deep section of the sample and the highest rates of activity coincided with the depth region where the highest oxygen levels were found Shope Summary of the results of the performed light dark shift measurements in the photosyn thetic mat environment 4 TROUBLESHOOTING Problem Possible cause 1 Possible cause 2 Solution Problem Possible cause 1 Solution Possible cause 2 Solution Instruments not found You have not connected your digital sensor instrument or A D converter An A D converter with different bit resolution has just been connected Open the configuration utility InstaCal InstaCal will clear any old A D converters not connected to your system and search for connected A D converters Noisy measurements Physical vibrations from other appliances on the table are causing movements of the sensor resulting in instability of experimental set up and disturbances in the measurements Remove all unrelated appliances from surfaces in contact with the experimental set up and ensure completely stable conditions for the sample Electric noise in the system Check that the system is properly grounded Connect the Ground connection on your sensor instrument to a ground source a waterpipe or similar Sometimes it can also help to ground the me
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