ÄKTAdesign Buffer Selection Kit
Contents
1. Bjorkgatan 30 SE 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Bucki GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 GE Healthcare Bio Sciences KK nghamhire HP7 9NA UK Piscataway NJ 08855 1327 USA Sanken Bldg 3 25 1 Huakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 e Austria Tel 01 57606 1619 Fax 01 57606 1627 Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 800 463 5800 Fax 800 567 1008 Central East amp South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 Denmark Tel 45 16 2400 Fax 45 16 2424 e Finland amp Baltics Tel 358 0 9 512 39 40 Fax 358 0 9 512 39 439 France Tel 01 69 35 67 00 Fax 01 69 41 96 77 Germany Tel 0761 4903 490 Fax 0761 4903 405 Italy Tel 02 27322 1 Fax 02 27302 212 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 9600 687 Fax 30 210 9600 693 Netherlands Tel 0165 580 410 Fax 0165 580 401 Norway Tel 815 65 555 Fax 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 South East Asia Tel 60 3 8024 2080 Fax 60 3 80242. 2090 Spain Tel 93 594 49 50 Fax 93 594 49 55 e Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 12 Fax 0848 8028 13 UK Tel 0800 616928 Fax 0800 616927 USA Tel 800 526 3593 Fax 877 295 8102 imagination at work User Manual 71 5029 21 AC 03 2006 E Elanders Tofters 2005
3. CAUTION Make sure AKTAdesign is switched off before installing the optional components The mains power to the system must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables Buffer Selection Kit User Manual 71 5029 21 Edition AC 10 2 Safety Buffer Selection Kit User Manual 71 5029 21 Edition AC Unpacking 3 3 Unpacking Unpack the optional components and check against the supplied packing ist Inspect the items for obvious damage that may have occurred during transportation CAUTION Read the following information carefully as well as all the additional instructions supplied with the components to ensure that the AKTAdesign optional equipment is installed correctly Buffer Selection Kit User Manual 71 5029 21 Edition AC 11 12 3 Unpacking Buffer Selection Kit User Manual 71 5029 21 Edition AC Pre requisites 4 4 Pre requisites The general procedures for creating and editing methods are described in the UNICORN User Manuals Note The system configuration delay volume must be recalculated and reset when the standard configuration after the UV flow cell is changed KTArr c AKTApurifier and AKTAbasic respectively must be installed and fully tested before the optional components are installed See the Installation Guide for your AKTAdesign chromatography system WARNING Ensure that the entire system has been flushed thoroughly with distilled w
4. GE Healthcare AKTAdesign Buffer Selection Kit User Manual O AKTA Important user information All users must read this entire manual to fully understand the safe use of Buffer Selection Kit WARNING The WARNING sign highlights instructions that A must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be col
5. atch Other Delete Ready NUM 3 Click the Flowpath radio button 4 From the Instructions list select BufferValveA The valve instruction parameter Position allows you to change all eight positions of the buffer valves individually Buffer Selection Kit User Manual 71 5029 21 Edition AC Operation 7 5 Define a variable name for the buffer selection instruction parameters e g BufferValveA_pos This variable allows you to turn the position of the buffer valve to the position you want Variable name definition Variable name BufferValve _Pos Cancel Clear Help 6 Click OK The defined variable is inserted in the method 7 Return to the Variables page by selecting View Run setup to set the variable BufferValveA_pos to the desired position 8 Save the method 7 3 AKTArrLc AKTApurifier AKTAbasic UNICORN 4 0 4 12 7 3 1 Using the buffer selection function in a run To use a valve in a method select the desired valve options in the Method Wizard when creating the new method The buffer valve instruction is designated BufferA In the Run Data pane they are designated V5_BufferA for KTArp c and V6_BuffA for AKTApurifier and AKTAbasic Buffer Selection Kit User Manual 71 5029 21 Edition AC 23 7 Operation 7 4 AKTApurifier UNICORN 5 10 or higher 7 4 1 Using the buffer selection function in a run To use a valve in a method select the desired valve options in the Method Wizard when creating the n
6. ater before removing any capillaries or components CAUTION Make sure that the AKTAdesign instrument is switched off before installing the optional components The mains power to the AKTAdesign instrument must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables Buffer Selection Kit User Manual 71 5029 21 Edition AC 13 14 4 Pre requisites Buffer Selection Kit User Manual 71 5029 21 Edition AC Mounting of brackets 5 5 Mounting of brackets CAUTION Make sure that the AKTAdesign instrument is switched off before installing the optional components The mains power to the AKTAdesign instrument must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables Optional equipment can be mounted on the system When selecting mounting location for the optional equipment it is important to choose a location which minimize the length of the capillaries used to connect the optional components to the rest of the system Many components that are attached to the mounting rails uses a snapin bracket The bracket is supplied separately with the component and needs to be fitted as shown below before the component can be attached 2 Fit the attached mounting bracket with the two screws Inserting components 1 Loosen the two screws Removing components Buffer Selection Kit User Manual 71 5029 21 Edition AC 15 5 Mounting of bracke
7. ew method 1 In the Method Editor select File Method Wizard and start creating a new method 2 Inthe Buffer Inlets dialog select A11 under Buffer Valve Inlet 3 Click Finish The buffer valve instruction is designated Buffer_A1 In the Run Data pane it is designated V6_Buffer Valve A1 for AKTApurifier 24 Buffer Selection Kit User Manual 71 5029 21 Edition AC www gehealthcare com lifescience GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweeden Drop Design KTA KTArp c KTApuri er AKTAbasic and UNICORN are trademarks of GE Healthcare companies GE imagination at work and GE monogram are trademarks of Genera Microsoft and Windows are either trad Electric Company lemarks or registered trademarks of Microsoft Corporation in the United States and or other countries All goods and services are sold subjec to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regu required to make changes in specifica discontinue the product described at any time without notice or obligation atory and contractual approval if tions and features shown herein or Contact your local GE Healthcare representative for the most current information 2005 General Electric Company Al rights reserved GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Bio Sciences AB
8. her e AKTApurifierTM with UNICORN Software 3 21 AA or higher e AKTAbasic with UNICORN Software 3 21 AA or higher Buffer Selection Kit User Manual 71 5029 21 Edition AC Safety 2 2 Safety IMPORTANT AKTA Buffer Selection Kit intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes e The components are designed for indoor use only e Do not use in a dusty atmosphere or close to spraying water e Operate in accordance with local safety instructions WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken WARNING Ensure that the entire chromatographic system has been flushed thoroughly with distilled water before removing any capillaries or components WARNING Always disconnect the power supply before attempting to replace any item on the equipment during maintenance WARNING If there is a risk that large volumes of spilt liquid may penetrate the casing of the equipment and come into contact with the electrical components immediately switch off the chromatographic system and contact an authorized service technician WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system gt Pe ee
9. ion valve you have installed by checking the box or by clicking ADD Click OK twice and then Close Mark the INV 908 valve with A Check that the ID code switch is set to 6 AKTAFPLC to 5 Mount the bracket and attach the valve to the system rack see chapter 5 Cut and mount the required capillaries Use a capillary with the correct i d as required by your system i d 1 6 mm and i d 2 9 mm Buffer Selection Kit User Manual 71 5029 21 Edition AC 19 20 6 Installation Buffer Selection Kit User Manual 71 5029 21 Edition AC 7 Operation Operation 7 7 1 Preparing for operation 1 The buffer selection valve is automatically recognized by UNICORN at system start up Check that the added valve is indicated in the Flow Scheme in UNICORN Verify that the valve is functioning properly by issuing manual commands from UNICORN as follows In System Control select Manual FlowPath Select instruction BufferValveA Set BufferValveA to position A4 in UNICORN 5 10 to position A14 Manually run Pump A with distilled water at a flow rate of approximately 2 ml min and check that water is drawn through port 4 in BufferValve A Buffer Selection Kit User Manual 71 5029 21 Edition AC 21 22 7 Operation 7 2 AKTAFPLC AKTApurifier AKTAbasic UNICORN 3 2 3 21 7 2 1 Using the buffer selection function in a run The buffer valve instruction is designated BufferValveA In the Run Data pane it is designated V5_BufferA fo
10. lected separately Please contact an authorized ME E resentative of the manufacturer for information concerning the decommissioning of equipment WARNING This is a Class A product In a domestic enuironment this product may cause radio interference in which case the user may be required to take adequate measures Contents Contents 1 Introduction Li Dl 7 12 Optional buffer selection valve 7 2 Safety 3 Unpacking 4 Pre requisites 5 Mounting of brackets Bel GIVE A ON 16 5 2 UniNet CONNECTION nn nee 17 6 Installation 6 1 Preparation for USE 19 7 Operation 7 1 Preparing for operation 21 7 2 AKTAreic AKTApurifier AKTAbasic UNICORN 3 2 S ld ds 22 7 2 1 Using the buffer selection function in a Fun 22 7 3 AKTAre c AKTApurifier AKTAbasic UNICORN 4 0 4 T2 nn 23 7 3 1 Using the buffer selection function in a FUN 23 7 4 AKTApurifier UNICORN 5 10 or higher 24 7 4 1 Using the buffer selection function in a FUN 24 Buffer Selection Kit User Manual 71 5029 21 Edition AC 5 Contents Buffer Selection Kit User Manual 71 5029 21 Edition AC Introduction 1 1 Introduction 1 1 General KTAdesign standard system configurations can be changed to optional configurations This flexibility in AKTAdesign system strategies allows the user to enhance already used purification methods and also to develop new more complex methods When optional equipment is connected to a standard chro
11. matographic system new sets of instructions to control the optional equipment become accessible for the user Optional configurations are selected installed and put into operation by the user An optional configuration consists of both hardware components and software instructions Optional configurations are monitored and controlled via methods run by the UNICORN control system in the same way as AKTAdesign standard system configurations 1 2 Optional buffer selection valve An optional buffer valve allows selecting automatically between eight additional buffers This functionality enables the following e Automated buffer reagent screening for purification optimization e Moving inlet tubing by hand is no longer necessary and the risk of introducing air into the system is removed e A solution of nickel sulphate connected through the buffer valve enables automated charging of metal ion affinity columns e Buffers for cleaning can be added and stored in place for different runs e Large volumes and multi sample applications can be run through one or several additional buffer valves Buffer Selection Kit User Manual 71 5029 21 Edition AC 7 1 Introduction ine filter Injection Valve V1 Pump A Pump B Buffer Valve A Fraction Collector Restrictor The optional buffer selection valve code no 18 1108 42 is available for e AKTAFPLC with UNICORN Software 3 2 or hig
12. r AKTArpic and for AKTApurifier KTAbasic it is V6_BuffA1 To use a valve in a method proceed as follows 1 Inthe Main Menu select File New Method to create a new method Select System Technique Template and For column Click OK 2 Select View Text instructions to display the text instruction editor Double click on the instruction block where you want to add the general function valve instruction e g Start_Conditions_zz Highlight the instruction below to insert the valve instruction Method Editor UNTITLED M Ex File Black View Adviser Help oa ala ls aj fal E Main 0 00 Base CV 0 10 Column ml Any E W 0 00 Block Start_Conditions Start_Conditions 0 00 Base SameAsMain Alarm_Pressure Enabled 4 0 Pressure_Limit MPa 0 00 MPa 0 00 AveraginglimeUY 2 60 UV_Averaging_time W 0 00 Block Initial_Eluent_Conditions 0 00 End_block W 0 00 Block Column_Equilibration W 0 00 Block Flowthrough Fractionation W 0 00 Block Sample_Injection W 0 00 Block Wash Out Unbound_Sample M 0 00 Block Eluate_and_Peak_Fractionation a a a 0 00 Block Gradient_Elution 0 00 Block Clean_After_Gradient 0 00 Block Reequilibration 0 00 End_ method 20000000 Click here to def ne a variable name for the instruction parameter Instructions InjectionValve Pasition Kat 7 C Pump Insert oo je roman E eva u War hs InjectionMark Var e NK Change C Frac Beplace C W
13. termination plug can be moved to the last component furthest away from the system pump if motivated by cable routing considerations WARNING Only use mains cables delivered or approved by GE Healthcare To PC controller board UniNet 2 Fraction chain collector Termination plug PC running UNICORN UniNet 1 chain Use the attached UniNet 2 cables to connect the valve to the UniNet 2 communication link The valve can be connected anywhere between the system pump and the termination plug connected to the outlet valve Check that all capillary and electrical connections are carried out correctly WARNING Do not block the rear panel of the system The mains power switch must always be easy to access Buffer Selection Kit User Manual 71 5029 21 Edition AC 17 18 5 Mounting of brackets Buffer Selection Kit User Manual 71 5029 21 Edition AC 6 Installation Installation 6 6 1 Preparation for use To start your AKTAdesign optional configuration system 1 10 Switch on the AKTAdesign instrument with the mains switch located at the front left on the system base Check that the computer and printer are switched on Log in see the instrument user manuals In UNICORN Main Menu Manager select Administration System Setup Select System and then click Edit Click Component From the Component list select the optional buffer select
14. ts 5 1 Valve ID The buffer selection valve has a unique ID code which identifies it to the UNICORN control system at system start up This ID code should be checked before installation The ID code for the buffer selection valve and the corresponding instructions used in UNICORN to control it are as follows Valve function ID code Valve type Instruction in UNICORN Buffer selection A KTAPPLC 5 INV 908 BufferValveA KTApurifier 6 INV 908 BufferValveA1 AKTAbasic 6 INV 908 BufferValveA1 Components are connected to the UNICORN control system using either UniNet 1 or UniNet 2 cables Both the UniNet 1 and the UniNet 2 data communication chain in standard configuration is routed from the rear of the system pump via their respective components to the last component in the chain where it is terminated with a plug Frac 920 Frac 950 A 900 and A 905 are connected via the UniNet 1 chain All other optional components are connected via the UniNet 2 chain Note When Pump P 960 is used it is always installed as the last component in the UniNet 2 chain Since the pump has an internal termination no termination plug is needed Buffer Selection Kit User Manual 71 5029 21 Edition AC Mounting of brackets 5 5 2 UniNet Connection Both the UniNet 1 and the UniNet 2 chain can be interrupted anywhere between the system pump and the termination plug to interconnect the optional components in the chain The
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