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NOTE - Harvard Apparatus

1. 76 IB cse E dores PE MR 76 CAD Mea hod Expottcand IDOE arma iio a Pte ema Punp Cono aneia i suptinmbetarant tia lease tacos cercos 79 natal Oho gles ha cial Koga o Loc A Nm 79 ME Tt Uca tt seca osetia 81 Usine the PAD ULTRA Pamp Chain OM as torpe 81 no 82 LOs a ETA T re eee ne nr E ee eee 83 A A E E E EAE EE AEE MR sinew EIA A 86 Rie ON e n T EAA E E neon 87 e Command naar 89 Tune e A PR PC E INE Det AET 90 D ALO area Eo A EE 92 rs Dn MA E A E A E S 93 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Table of Contents 5 APO ICO aa 94 Appendix A Sytinee Volume Diameter Reference Table 94 Appendix Bx sinless Siel STINGES secerni inihi pii pia Ei i Aaa ei in 95 Appendix Ci Miny Max Flow Rate sedes 96 P nA Since Holder O A Cen E E O E E A EIUMOD 97 Mountno a 4x140 Multi RACK atera pita ie 97 Monadas d cy TO Mula ebria bio podio iia 99 Syringe Loading 4 x 140 amp 6 10 Mall RACKS auisseietqittitqptdid idiot Deas atesttes ttes etas 101 Mounting a Microltet Mald RaCk erro Meat RIVERA SUPER M npe tu pM UI EX OV RU RUE 103 Sytinee Loading Microliter Multi Rack usse rr betrert pb tit irc 105 ovine e Loading Pus Pol NIC AH SED prada 106 ppendi 1 Pta Leone ela esa io aran nere E eee 108 User VO Connector SpeciNCa ONS ias bald otero 108 alos AU slap iia 109 b a sate EE
2. 110 RSS PO CAOS A T AEE AEE 112 rs E E A A A E E IIA 113 liri T neta eke Ur O H 114 Remote Gradient gt cd tetitas 115 Connectine a Remote Satellite Pump eones 115 Setting Addresses on Remote Satellite Units nenas 116 Setting the Address on the Control Box Master Uan nasa 116 os AA oo E a i 118 E A II 118 use Replace DEDE o Duis auesniis astu das tuatia MIA oe 119 DattercoBeplacei I emo o E E T E 119 Te UDEO CPP oo NEEISREDIBNEMIURRNRERUEI CH CENE ENSIS DePDININ EN USO SUD IN 119 Tron mi BOO ERE RR m 120 Uperadino 1 24 PRO ota datada on ao 121 PHD WR Display Brot Messages ausiai a divinos 122 DDpeHde Ga ate e DOSES enreda eb rere renee eee eee Cee 123 Master sh colt vem Contato enano llena aeaaea 124 Optional PG t0 Pump Conti utadions siaina aai 125 feti rentaibdiuM esto come ch 06 RTT A 126 o e En E uM ce ee EUN 128 Dppendis To toc Pall VAS cases UNT EA OMA QUEM PU pUNES 129 I Pe X H 129 appeal Ordene ornato 130 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Quick Guide 6 Quick Guide 10 THINGS YOU NEED TO KNOW TO USE THE PHD ULTRA Since many of our users are already familiar with the operation of a syringe pump we ve created this one page summary of important tips for using the PHD ULTRA Operations Display this section of the screen is used to display Message Area this section is u
3. 4 eeeeeeeeeeeee eere nenne nennen nnne 43 Schn A Time Delay For Method Opcion meat act 45 Cranc an EA PRO New Me bolsones R E EOE 46 Modifying 4 Preloaded User Defined MethOdessissrisisorissadissasnssascssiinsinnasiannn ekses 48 Changing The Order ol Stepi ma Method serros 48 Delete a Siep Foma Metodos 48 IDupheating a otep lna Me od sanan ion 49 Pennine Method Stop POI TeTS anan AA AE EARN EN 49 PPOP ci Pro E aae aaa 50 Constante Rate POE ono NA A A A EE EO OOE 5l I ia rou Me e A E E T o E 22 Biebbed PIOHIS s uenenum entrare Me EE dI Hed D tr te 54 EA RR po es essa sede de deems 56 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 3 Table of Contents 4 Dolus Pro A ice 58 Concentration Pro le roba 59 radica Proteatro plan A A 62 oe A En E o y eo 65 Incorporating Advanced Options Commands and I O Into A Method 67 ors et 67 Ip e 68 e a E E ees 69 Era ei A e O RE O ES ESSE ESE OA 70 vo PEREA ER Un e on E E A T TS 70 MOY MENG THOS 0 O o 71 POT AAA o cee rere EeTeEerN ETN me MEATS reer Teer 71 DOS castes eee 13 A A PP o Un EREE 74 Marac no PRO Melo ds amnesia pea fs Sima a OL IT aria napa 75 PRS EN TO M c ENEE A OEE 19 ojo rote late WU Ts dato a e
4. TO CREATE A NEW USER DEFINED METHOD 1 From the Method Main or Quick Start screen choose the Method Select button to enter the Method Selection screen 2 Select New Method then choose Accept or press New Method again Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 47 3 Onthe Method Name screen type the name of your Method up to 15 characters using the onscreen keyboard Choose the Symbols Numbers button 123 to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters mode choose the Letters button ABC FS EE E ERN E ERR ERE Ll Li Li Li i New Method Name Entry screen showing letter keys To Be 18 E e E GUESS co number mos choose tbe 123 button in 2 20 6 1 8 5 M1 e mer 2123 space ello 4 When you have entered the desired name for your Method choose the Accept button to return to the Method Main screen Your new Method will now be listed in the Method Select button 5 Choose the Syringe Select button and complete the selection of the syringe by following the procedure outlined previously in the Syringe Selection section of this Guide 6 To begin creating Pump Operation Profile Steps choose the Step Definition button to enter the Method Steps Summary screen 7 To add a Step to your Method choose the Add Step button to access the Select Prof
5. NOTE Refer to Appendices for additional instructions on assembly of the Harvard BZ Apparatus Multi Rack Accessory 1 Raise or remove Clamp Bar 7 by unscrewing thumb screw 2 Loosen T Knobs 8 and slide the Retaining Bracket 4 on the Syringe Holder 3 open 3 Adjust the Pusher Block 2 to the approximate length of the syringe s by squeezing the Release Tabs 1 and sliding the Pusher Block into position 4 Loosen the three thumb screws on the Anti Syphon Bar 5 to provide enough room for the plunger flange of the syringe s If using the 6 x 10 rack loosen the two thumb screws on the outside of the Plunger Holder 11 Flip the plunger holder open 5 Place the syringe s on the Syringe Holder 3 ensuring that the barrel flanges are within the Syringe Holder s Retaining Bracket 4 and the plunger flanges are between the Pusher Bar 6 and Anti Syphon Bar 5 NOTE If using fewer syringes than the maximum number of syringes try to place them 2 symmetrically about the lead screw to present a balanced load 6 Push the Syringe Holder s Retaining Bracket 4 tight against the barrel flanges and tighten into place using the two T Knobs 8 7 Tighten the Anti Syphon Bar 5 using the three thumb screws If using the 6 x 10 rack flip the Plunger Holder 11 back into place and secure by tightening thumb screws 8 Lower or replace the Clamp Bar 7 and tighten thumb screw until Clamp Bar is firm against syringe s
6. Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 85 Using The PHD ULTRA DIM Sets or displays the backlight level in percent Valid range is O to 100 Note that specifying O will turn the backlight off so that the screen will not be visible Command format dim 0 100 Query response If Backlight is set to lt cr gt Ift prompt ECHO Sets or displays the RS232 USB echo state Valid states are on or off Command format echo on off Query response If Echo is OFF lt cr gt It prompt Of If Echo is ON lt cr gt It prompt FREE Displays the number of free method steps left on the disk Command format free Query response Ift steps used lt cr gt If steps free lt cr gt If total steps lt cr gt It lt prompt gt The number of steps displayed is a four digit number right justified and padded with spaces FORGE Sets or displays the infusion force level in percent Valid range is 1 to 100 Command format force 1 100 Query response lt lf gt lt cr gt lt lf gt lt prompt gt Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 84 Using The PHD ULTRA 85 METRICS Displays the pump metrics The operation of this command is covered in the PHD ULTRA Configuration document
7. Release Tabs 4 Bracket Clamping Knob 7 Retaining Bracket 9 T Knob Syringe Holder Pusher Block 5 Thumbscrew Cap 10 Bumper u Syringe Holder Retaining Bracket 6 Screw Cap 11 Clamp Bar Assembly Pusher Block Front view of PHD ULTRA showing controls for microliter multi rack syringe loading Squeeze Release Tabs 1 and adjust the Pusher Block 2 to the approximate length of the syringe s Loosen the Retaining Bracket 3 on the Pusher Block 2 by unscrewing the Bracket Clamping Knob 4 Loosen the Retaining Bracket 7 on the Syringe Holder 8 by unscrewing the two T Knobs 9 pu O qw x Create room for the syringe s by unscrewing the Screw Caps 6 until the Bumpers 10 are at their maximum height 5 Slide the syringe under the Bumper 10 placing the barrel flange within the Syringe Holder s Retaining Bracket 7 and the plunger flange within the Pusher Block s Retaining Bracket 3 NOTE If you are using syringes larger than 1 ml it may be easier to unscrew one of Y the Thumbscrew Caps 5 and swing the Clamp Bar Assembly 11 to the side making more room to load the syringe s After the syringe s are in place swing the Clamp Bar back over the Syringe Holder 8 and reattach the Thumbscrew Cap 5 Lower the bumpers 10 until they are firm against the syringe s by screwing in the Screw Cap 6 Push the Syringe Holder s Retaining Bracket 7 tight against the barrel flange and tighten into place usin
8. Satellite RS 485 Remote 0 25 0 05 0 5 ul 140 ml 1 56 pl min 215 8 ml min N A Stores all settings N A IEEE 1394 6 pos Type B 15 pin D Sub Connector Option 34 kg 75 Ibs Y 100 Force Selection 0 9 Stepper Motor Controlled Microprocessor with 1 16 microstepping 12 800 27 5 sec ustep 26 usec ustep 0 18 um min 190 80 mm min 12 30 VDC 5 Input Power at RS 485 N A 11 75 x 5 5 x 6 5 in 29 8 x 14 0 x 16 5 cm 4 8 kg 10 49 Ibs 4 C to 40 C 40 F to 104 F 10 C to 70 C 14 F to 158 F 20 to 80 RH non condensing Continuous Class 1 lI Harvard Apparatus 84 October Hill Road Holliston MA 01746 CE ETL UL CSA WEEE EU RoHS amp CB Scheme ANSI UL 61010 1 2004 Ed 2 Rev 2005 CAN CSA C22 2 No 61010 1 2004 Ed 2 IEC 61010 1 2001 Ed 2 Corrigendum 1 2002 Corrigendum 2 2003 CENELEC EN 61010 1 2001 CB Scheme CENELEC EN 61326 1 2006 Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 12 SPECIFICATIONS Setting Up The PHD ULTRA With EZ PRO PHYSICAL VIEWS The following diagrams show the important components of the PHD ULTRA pump Fuse Holder Connector for Remote Mechanism option Analog Control Connector option lt Allen wrench RJ11 Connectors for RS 232 option Main Power Switch AAT a de User I O Connector Serial Number Label PS j a Footswitch Input E switch sold s
9. Secure the syringe clamp 3 and tighten using the Thumb Nut 8 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 107 PHD ULTRA showing syringes installed on both sides of the Pusher Block NOTE For Syringes lt 10ml you will need to remove the Thumb Nut 8 and then yz remove and flip over the Syringe Clamp 3 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual APPENDIX E EXTERNAL CONNECTIONS User I O Connector Specifications All Inputs are pulled high to 5 Vdc through a 10k resistor All Outputs are at TTL Logic Levels Trigger 1 Out Trigger 2 Out Footswitch In Sync Out Event In Direction S Birsetiondh Valve S 34 Run Indicator Run Indicator or Voltage User I O Connector Specifications Publication 5419 002 Rev E Appendices 108 Pin Assignments 1 Direction control input Rising edge sets pump to infuse Falling edge sets pump to refill 2 Trigger Input Event trigger falling edge triggers a program event 3 Footswitch Input Momentary switch closure to ground or TTL Logic Low falling edge toggles between states run stop 4 Trigger 1 Output TTL Logic Output Default Low Control thru method or serial comm 5 Trigger 2 Output TTL Logic Output Default Low Control thru method or serial comm 6 Sync Output TTL Logic Output Rising Edge Start Infuse Falling Ed
10. Spacer Micro Clamp 2 5015 035 T Knob 2 5100 05 Spring Washer 2 Micro Holder 5110 003 _ 2401 080 Micro Retainer 2401 081 Bracket Syringe Clamp 2401 050 Knob Syringe Clamp Dual Syringe Holder 2401 01 Microliter Multi Rack Syringe Holder Assembly Remove the four Set Screws from Syringe Holder using provided hex key Remove Syringe Clamp and Knob from the Syringe Holder First assemble the Retaining Bracket to the Micro Holder using the two T Knobs Place the assembled Micro Holder onto the syringe holder and screw in the two Standoff Bars c A e a Place the Clamp Bar Assembly onto the Micro Holder by screwing the Thumbscrew Caps into the Standoff Bars Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 104 Retaining Ring 5007 038 Shaft Bracket Clamp 2401 028 Bracket Anti Syphon 2401 025 Wosher Flat 2 5110 038 Knob Bracket Clamp 2401 031 Ext Tooth Lockwasher a 5174 003 Bracket Micro Ant syphon 2401 079 Microliter Multi Rack Pusber Block Assembly 5 Remove the Pusher Blocks Retaining Bracket by unscrewing the Bracket Clamping Knob completely and removing the Clamp Shaft 6 Attach Anti Syphon Bracket using the Clamp Shaft and Bracket Clamping Knob Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 105 Syringe Loading Microliter Multi Rack
11. Syringe Pump Series User s Manual Appendices 98 Ext Tooth Lockwasher 2 5174 003 Shaft Bracket Clamping 2401 028 Retaining Ring 5007 038 Bracket Anti Syphon 2401 02 e a X Thumbscrew 3 5100 012 Washer Flat 2 5110 038 Knob Bracket Clamping 2401 031 Pusher Bar 2401 057 Spring a 5009 164 S Washer Flat 5143 003 Bracket Anti Syphon 2401 069 Flat Head Screw 2 5137 015 4x140 Multi Rack Pusher Block Assembly 1 Remove Dual Syringe Anti Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft 2 Attach the Pusher Bar assembly to the Pusher Block using two flat head screws Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Mounting a 6 10 Multi Rack Knob Mulfi Syringe Clamp 2401 0 Post Clamp Guide 2 2401 078 Knob Syringe Clam 2401 051 T Knob 2 5100 059 6 10 Multi Rack Syringe Holder Assembly poe ues Multi Holder Publication 5419 002 Rev E Appendices 99 ong Bar 2401 072 810 32x 1 2 Screw 4 5101 087 Multi Holder 2401 073 Multi Retainer 2401 075 Bracket Syringe Clamp 2401 050 Lock washer 2 5174 003 ee Flat Washer 2 j 5110 038 Dual Syringe Holder 2400 238 Remove the four Set Screws from Syringe Holder using the provided hex key Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob Place the Multi Ho
12. Command format vduty 5 80 Query response It 96 cr If prompt VER Displays the short version string Command format ver Query response If PHD Ultra cr It prompt VERSION Displays the full version string Command format version Query response lt lf gt Firmware v cr If RTOS FreeRTOS v http www freertos org lt cr gt lt lf gt Pump address lt cr gt lt lf gt lt prompt gt RUN COMMANDS IRUN Runs the pump in the infuse direction Quick Start mode only Command format irun RRUN Runs the pump in the opposite direction Quick Start mode only Command format rrun RUN Simulates a key press of the run button located in the lower right corner of the screen Command format run Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 86 Using The PHD ULTRA RUN COMMANDS CONTINUED STOP STP Stops the pump Command formats stop stp WRUN Runs the pump in the withdraw direction Quick Start mode only Command format wrun RATE COMMANDS CRATE Displays the current rate that the motor is running at A valid response is returned only in dynamic situations while the pump is running Quick Start mode only Command format crate Query response lt If gt Infusing at xl xxx lt cr gt If prompt O
13. HARVARD APPARA TUS 04 28 09 1 56 40 PM Enter your settings to the left and press the RUM or Run Preview HA steel 4 851 mm 2 5 ml button Make sure to select the appropriate syringe EO Quick Start Infusion Only The Time Date area turns red when the battery is low 3 525 ml min Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 123 APPENDIX G SATELLITE BOXES Allen wrench Locking Spring for Din Rail Mounting Groove for Din Rail Mounting Back view of Satellite box Guide Rods Half Nut Release Pusher Block 224 screw Syringe Barrel Clamp Syringe Holder Vertical Foot Support Bracket Clamp Knobs Mechanical Stops Front Top view of Satellite box Run LED ag User I O Connector DIGITAL VO Option a USB Serial Input USB kes JO For communication from PC RS 485 Connectors For pump to pump communication Close up of Satellite box connections Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 124 Master Satellite Configurations PC optional STAND ALONE STAND ALONE STAND ALOME Mote 1 MASTER SATELLITE SATELLITE ADDR t ADDR 01 ADDR 07 AC Power R 485 RS 495 R5 485 OUT IN OUT IN MUST BE ADDA ADDRESSES CAN BE 01 99 Master Satellite operation using stand alone master units as satellites P
14. HARVARD APPARATUS 04 28 09 1 48 28 PM Enter the step settings Step Definition screen for a Bolus Delivery Profile Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 59 TO SET UP EDIT A BOLUS DELIVERY PROFILE 1 From the Step Definition screen for a Bolus Delivery profile choose the Set Target Volume button 2 On the Set Target Volumes screen enter the desired value using the keypad 3 Ifthe Units of Measure are not correct for your application choose the Units button to enter the Units selection screen Select the unit of measure button ml ul nl pl applicable for your application then choose the Accept button to return to the Set Target Volumes screen 4 When the Volume parameters are correctly set choose the Accept button to return to the Step Definition screen 5 Select the Target Time button On the Set Target Times screen enter the desired value using the keypad If the Units of Time are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 8 When the Time parameters are correctly set choose the Accept button to return to the Step Definition screen The pump will automatically calculate the flow rate based on the entered Target Volume and Time CONCEN
15. If the Units of Volume associated with Volume 1 or Volume 2 are not correct for your application choose the Units button associated with either parameter to enter the Units selection screen Select the unit of measure button ml ul nl pl applicable to your application then choose the Accept button to return to the Set Pulse Volumes screen When both Volume parameters are set to the correct values choose the Accept button to return to the Step Definition screen Select the of Pulses button to enter the Set Pulses screen On the Set Pulses screen enter the desired value using the keypad then choose the Accept button Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 58 BOLUS PROFILE The Bolus Delivery profile is used to infuse a defined dose of fluid controlled by the total volume and the total time the pump operates Bolus profiles are used for infusion only In a bolus profile you set both the time the pump operates and the total volume to be delivered The pump automatically calculates the flow rate based on the syringe selected The flow rate is constant during a Bolus Delivery profile Bolus Delivery Pump Operation Profile Time Volume When creating or editing a Bolus Delivery profile you will define the following operating parameters e The Target Volume to be infused e The Target Time value which is the total time the pump will operate
16. If the pump address is nonzero the one or two digit pump address precedes the command without any separator For example to set the infuse rate for pump 12 the command would look like 12irat 3 2 u m If the pump address is one digit it doesn t have to be preceded by a 0 Note that if the USB port is used on a pump with a nonzero address the pump address doesn t need to be used Every line of the response from a pump with a nonzero address is prefixed by the two digit pump address prefixed with a 0 if necessary followed by a colon No colon is used to separate the pump address from the prompt however In the command list below the following convention is used U Required parameter Optional parameter Separator between parameter choices Numeric value without preceding zeros HHH Numeric value with preceding zeros HA A range of values Cr Carriage return Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 82 PHD ULTRA Pump Chain Commands continued If Line feed sp Space prefix Pump address prefix in the format if the pump address is not zero prompt Prompt see below The following prompts are returned after a command is executed The pump is idle gt The pump is infusing lt The pump is withdrawing The pump stalled T The target was reached If the pump is in poll mode an XON character is added after the prompt If the
17. Syringe Pump Series User s Manual Using The PHD ULTRA 66 TO SET UP EDIT AN AUTOFILL CYCLING PROFILE 1 10 11 12 13 14 15 16 From the Step Definition screen for an Autofill Cycling choose the Set mode button to toggle between the Withdraw Infuse WD INE and Infuse Withdraw INF WD modes Withdraw Infuse begins by withdrawing into the syringe following by infusing into the target Choose the Set Rates button to enter the Set Infuse Withdraw Rate screen Select the Infuse Rate it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired rate using the keypad or select the Minimum Rate or Maximum Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pb and the unit of time sec min hr as appropriate then choose Accept to confirm your selections Select the Withdraw Rate and enter the desired rate using the keypad or select the Minimum Rate or Maximum Rate button When all parameters are correctly set on the Set Infuse Withdraw Rate screen choose Accept to confirm your settings and return to Step Definition screen Choose the Set Volume per Cycle button to enter the Set Volume per Cycle screen Enter the Volume using the keypad If the Units of Measure associated with Volume are not correct for your application choose th
18. ml min 100 205 nl min Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 10 11 12 13 14 15 16 L7 18 Using The PHD ULTRA 61 Select the Infuse Rate it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired rate using the keypad or select the Min Rate or Max Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pb and the unit of time sec min hr as appropriate then choose Accept to confirm your selections Select the Concentration button and enter the concentration of the active ingredient in the infusion fluid in mg ml When all parameters are correctly set on the Set Rate and Concentration screen choose Accept to confirm your settings and return to Step Definition screen Choose the Set Dose or Duration button to enter the Set Dose or Duration screen On the Set Dose or Duration screen select either the Dose button if you wish to enter the target dosage value in which case the PHD ULTRA will calculate the required pumping duration or Duration if you wish to infuse for a specific time period in which case the PHD ULTRA will calculate the delivered dose As appropriate enter either the Dose in mg ml or the Duration using the keypad If the Units of Time ass
19. 3 Choose the File Options button to enter the File Options screen 4 Choose Append to access the Method to Append screen 5 Choose press twice the Method you would like to Append attach to your base Method The pump will return you to the File Options screen and display a confirmation that the Method was copied 6 Choose Back to return to the Method Selection screen Deleting A Method Follow these instructions to delete a previously saved Method 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want to delete then choose the File Options button to enter the File Options screen 3 Choose Delete Method The application will display a warning message in the Message Area asking you to confirm your deletion request 4 Choose Delete Method again to confirm the deletion and return to the Method Selection screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 71 PHD ULTRA Method Export and Import The PHD ULTRA pump permits the daisy chaining of multiple pumps as well as integration with control by an external computer through HyperTerminal To facilitiate operation in these modes the PHD ULTRA application software includes a variety of commands designed to simplify the export import of Methods between the pump and external d
20. Command format metrics POLL Sets or displays the polling mode state In polling mode prompts are inhibited if they aren t generated by a typed command Valid states are on or off Command format poll on off Query response If Polling mode is OFF lt cr gt Ift prompt Of lt If gt Polling mode is ON lt cr gt It prompt TILT Calibrates the tilt sensor Command format tilt TIME Sets or displays the date and time Command format time mm dd yy hh mm ss Query response lt If gt 4 4 HEHEHHE M lt cr gt lt lf gt lt prompt gt The date order format is dependent on the settings made when setting the date from the touch screen The time format is 24 hours VALVE Displays or sets the valve state When the valve is set to auto the motor direction determines the valve state Valve defaults to Auto on power up Command format valve on off auto Query response Ift Infuse cr Ift prompt Of lt lf gt Refill lt cr gt lt lf gt lt prompt gt Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA VDUTY Sets or displays the valve duty cycle in percent Valid range is 5 to 80 Default value is 50 6 If a valve actuates but does not hold in actuated position vduty command should be used to increase duty cycle percentage to allow valve to hold
21. NOTE For Infuse Withdraw Methods this parameter only permits the setting of the 2 Target Volume parameter TO SET THE TARGET VOLUME OR TIME 1 From the Quick Start screen choose the Target Volume Time Select button 2 Onthe SetTarget Volume or Time screen select the Volume or Time button as desired 3 Enter the desired value using the keypad or select the Maximum Volume button 4 If the Units of Volume Time are not correct for your application choose the Units button to the right of the parameter entry box For the Units of Volume this choice takes you to the Units Selection screen For the Units of Time option this choice will toggle the button between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 5 If you are changing the Units of Volume on the Units Selection screen select the unit of measure iml ul nl or pb as appropriate then choose Accept to confirm your selection 6 When all parameters are correctly set on the Set Target Volume or Time screen choose Accept to confirm your settings and return to the Quick Start screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 42 USING AN EZ PRO PRELOADED USER DEFINED METHOD The Preloaded Methods and User defined Methods each use the PHD ULTRA Ss integrated tools that guide users throu
22. Review the Touch Screen Button Reference on Page 17 of this manual to familiarize yourself with the onscreen navigation commands Once you know the buttons you ll be able to navigate freely in the system 2 Select and Choose Menus on the PHD ULTRA that require selection from a list use a persistent double touch to select an item That means that you will touch the desired item once to highlight it then a second time to confirm your choice Most other commands only require that you touch one time to choose that function 3 Home The Home screen in the PHD ULTRA software is the Method Main or Quick Start screen This screen displays the currently selected Method along with its current parameter settings To change Methods choose the Method Select button 4 RunningA Method To run a Method choose the Run button It will point left or right depending on whether the first operation is a Withdraw left or Infuse right Step If all the required parameters for the Method are not set the Run button will be inactive grayed out 5 Lost If you start navigating and suddenly you feel lost choose the Cancel button or the Back Button Do that enough and eventually you will be back to the Method Main screen or Quick Start screen 6 Watch the Force The PHD Ultra generates up to 75 lbs of force enough to damage delicate syringes A Force Limit command in the Settings menu allows you to control the maximum force value 7 GetThe
23. or choose the Cancel button to cancel your changes and return to the Method Main screen 4 Review the Pump Operation Profile Steps described in the Step Definition button Note that the software will display the first three Steps in the Method in the main button area If the Method uses more than three Steps the last Step in the Method will be displayed in the box beneath the Step Definition button 5 To review the Method Steps in detail or to update modify any of the stored Pump Operation Profile Parameters choose the Step Definition button to enter the Method Steps Summary Screen The name of the Method is listed at the top of the screen and the complete list of Steps is listed in a series of buttons beneath the Method Name HARVARD APPARA TUS 04 28 09 1 13 35 PM Ramp m Select desired step You can add 3 295 ml rin 3 335 nl rmin delete move up down or set time delay Press the Back button Step Definition Screen to return to the previous screen showing the list of Steps currently defined for tbe Metbod 3 285 ml min 6 To review details for any individual Step in the Method select the Step you wish to review then press the Step button again to enter the Step Definition screen for that Step NOTE To edit the existing profile press the left side of the step button To replace this Ze step with a new profile press the right side of the button on the picture 7 To edit the parameters for any Pump Operation P
24. 9 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the right until the syringe plunger is located as far into the syringe as it will travel Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 102 10 Using the Allen Wrench located in its holder in the rear of the Pulley Cover 12 loosen the Infusion Mechanical Stop 9 and slide it to the left until it is in contact with the Pusher Block 2 Use the Allen Wrench to tighten it in this position 11 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the plunger is located just to the left of the maximum volume allowable Use the Allen Wrench to loosen the refill Mechanical Stop 10 and slide it to the right until it is contact with the Pusher Block 2 Tighten the Withdraw Mechanical Stop 10 and then return the Allen Wrench to its holder 12 4 x 140 Multi Rack 6 10 Multi Rack Multi Rack Configurations NOTE When multiple syringes are loaded it may be difficult to manually move the EP Pusher Block in order to set the Mechanical Stops Instead use a single empty syringe to measure the proper lengths and set both the Infuse and Withdraw Mechanical Stops first Then use the above procedure to load your syringes Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 105 Mounting a Microliter Multi Rack Clamp Bar Assembly 2400 290
25. Group 1 Class A EMC and Safety compliance were evaluated by Intertek ETL Semko Reference test report file numbers 3177745 BOX 001 003 004 005A 0064 007B 319599B BOX 005a I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date May 15 2009 A tl p C Signature HARVARD E APPARATUS VP Engineering Operations Position
26. October Hill Road Holliston MA 01746 CE ETL UL CSA WEEE EU RoHS amp CE ETL UL CSA WEEE EU RoHS amp CB Scheme ANSI UL 61010 1 2004 Ed 2 Rev 2005 CAN CSA C22 2 No 61010 1 2004 Ed 2 IEC 61010 1 2001 Ed 2 1 2004 Ed 2 IEC 61010 1 2001 Ed 2 Corrigendum 1 2002 Corrigendum 2 2003 CENELEC EN 61010 1 2001 CB Scheme CENELEC EN 61326 1 2006 Introduction To The PHD ULTRA 11 Remote 0 25 0 0596 0 5 ul 140 ml 1 56 pl min 215 8 ml min 4 3 WQVGA TFT Color Display w Touch Screen Stores all settings 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 34 kg 75 lbs Y 100 Force Selection 0 9 Stepper Motor Controlled Microprocessor with 1 16 microstepping 12 800 27 5 sec ustep 26 usec ustep 0 18 um min 190 80 mm min 100 240 VAC 50 60 Hz 75 W 0 5 A fuse 12 0 x 8 5 x 4 0 in 30 5 x 21 6 x 10 2 cm 11 0 x 5 3 x 6 5 in 27 9 x 13 5 x 16 5 cm 6 1 kg 13 4 Ibs 4 C to 40 C 40 F to 104 F 10 C to 70 C 14 F to 158 F 20 to 80 RH non condensing Continuous Class 1 Harvard Apparatus 84 October Hill Road Holliston MA 01746 CB Scheme ANSI UL 61010 1 2004 Ed 2 Rev 2005 CAN CSA C22 2 No 61010 Corrigendum 1 2002 Corrigendum 2 2003 CENELEC EN 61010 1 2001 CB Scheme CENELEC EN 61326 1 2006 Fan option is required if external operating ambient is expected to be gt 35 C Publication 5419 002 Rev E
27. RATE PROFILE The Constant Rate profile is used to run the pump at a defined flow rate and for a specific pumping mode Gnfuse or withdraw up to a target volume or for a defined time period Target Time or Total Volume Point Constant Rate Pump Operation Profile Time Volume When creating or editing a Constant Rate profile you will define three primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw e The Flow Rate to define the rate of uptake or dispensing e The Target Volume or Target Time that determine how long the pump will operate at the set rate When creating or editing a Constant Rate profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse and Withdraw Rate screens HARVARD APPARA TUS Enter your settings to the left and then press the Enter button 0 ml min green check mark to accept or press the Cancel button red X to return to the previous screen Step Definition Screen for a O ml Constant Rate Profile TO SET UP EDIT A CONSTANT RATE PROFILE 1 From the Step Definition screen for a Constant Rate profile choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation 2 Choose th
28. The first integer is the current rate in femtoliters per second The second integer is the infuse time in clock cycles One clock cycle is 1 60 000 000 second The time has a granularity of 1 millisecond The third integer is the infused volume in femtoliters All three values are for the current direction The flag field consists of six flags Flag one is the motor direction and will be I if the pump is infusing and w if the pump is withdrawing If the letter is lower case the pump motor is idle If upper case the pump motor is running Flag two is the limit switch status If the infuse limit switch was hit I is displayed If the withdraw limit switch was hit W is displayed If no limit switch was hit or the pump does not have limit switches Is displayed Flag three is the stall status and will be S if the pump has stalled Otherwise it will be Flag four is the trigger input state and will be T if high and if low Flag five is the direction port state and will be I for infuse and W for withdraw Flag six is the target reached status It will be T if the target time or volume was reached and if not Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 94 Appendices APPENDIX A SYRINGE VOLUME DIAMETER REFERENCE TABLE SGE Scientific Glass Hoshi Engineering Cadence Science Inc Harvard Apparatus F
29. User s Manual Using The PHD ULTRA 68 DELAY The Delay Command is used to insert a time delay in a procedure You can program a time delay between 0 2 seconds up to 99 99 99 99 hours 99 minutes 99 seconds TO INSERT A TIME DELAY IN A METHOD 2 es X d m From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the time delay to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen Choose Delay to enter the Set Delay screen HARVARD APPARATUS Time scs 04 28 09 1 40 53 PM Enter time delay and then press the Enter button green check mark ta accept ar press the Cancel button red X ta cancel our settings and return ta the Advanced Options Set Delay previous screen screen Enter the desired Time using the keypad If the Units of Time associated with Time are not correct for your application select the Units button to toggle between sec seconds 0 2 seconds minimum allowable value and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon You can enter a value up to 99 99 99 When your Time entry is correct choose the Accept button to insert the Delay Step in the Method and return to the Method Steps Summary screen Publication 5419 002 Rev E
30. ar Run Preview button Press the Method Delay button clock on paper to set a delay o e ic oo o gt us TTI 4 5 6 MEAE Lo s HARVARD APPARATUS 04 28 09 1 17 31 PM Select format enter date immidd and time then press the Enter button green check mark to accept or press the Cancel button red X ta cancel vour settings L1 SETTING A TIME DELAY FOR METHOD OPERATION 1 Set Delay Parameters button on Quick Start screen Set Delay Parameters button on Method screen Set Delay Date and Time screen From the Method Main or Quickstart configuration screen choose the Set Delay Parameters button to enter the Set Delay Date and Time screen To enter the date select the Date button and type the desired date using the onscreen number pad Select the Date Format button to toggle between Month Day Year m d y Day Month Year d m y and Year Month Day y m d formats Enter your values in two digit format for each value For example April 3 2009 would be entered as 04 03 09 in m d y format To enter the time select the Time button and type the desired time using the onscreen number pad Select the Time Period button to toggle between AM PM and 24 hours settings Time should be entered using hours minutes seconds hh mm ss Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 46 4 When the desired values are en
31. completed the required entries for Advanced Options choose the Accept button to save your changes and return to the Method Steps Summary screen 11 Repeat items 7 10 in this procedure to include additional Steps in the Method NOTE When adding a Step to a Method the Step is added after the currently selected A Step i e the Step that is highlighted in purple 12 When you are done adding all required Steps to the Method choose the Back button to return to the Method Main screen Modifying a Preloaded User Defined Method Once you have created a Method or have reviewed a Preloaded Method you may have a need to modify the structure of the Method i e the types of Steps and Commands included not just changes in the operating parameters for a specific Step The following procedures will help you to efficiently modify a stored Method CHANGING THE ORDER OF STEPS IN A METHOD 1 From the Method Main screen choose the Step Definition button to enter the Method Steps Summary screen 2 Highlight select with a single touch the Step for which you want to change the order 3 Choose the Move Step Up or Move Step Down button as required to move the Step into the position that you want it executed NOTE If there are Method Commands e g Go To that link to the Step moving Steps BZ can result in unexpected results as the Command may be linked to the Step number You should always recheck all Method Commands after adding deleting or
32. device The application software provides convenient selection of common syringe models from a Syringe Lookup Table as well as insertion of custom syringe specifications The system is then able to calculate the cross sectional area of the syringe selected and calibrate the flow rate and volume accumulation displaying real time operating characteristics via on screen graphics PUMP MODELS The PHD ULTRA Syringe Pump is available in three models designed for different operating environments and varying degrees of operational flexibility e Infusion Only This model supports infusion operations at user definable flow rates and with selectable target volume or time values to control the total infusion volume The entry level Infusion Only model does not include programmable user defined methods e Infusion Withdrawal This model supports infusion and withdrawal operations at user definable flow rates and with selectable target volume or time values to control the total volume pumped for both the infusion and withdrawal portions of a procedure This model provides access to many of the advanced method programming options of the PHD ULTRA but only supports a single stored Method e Infusion Withdrawal Programmable This model supports both infusion and withdrawal operations and can use both simpified pumping profiles or the more advanced pumping profiles and I O settings that permit interactions with external devices In addition to supporting
33. enter the Event Step Definition Screen Select either Input High Input Low or User Press as appropriate cu ME s GONN Le uem Choose the Accept button to save your changes and return to the Method Steps Summary screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 74 I O EVENT LINK IN The I O Event Link in command is used to have the pump jump to a linked method if the signal is received The external signals are communicated via Pin 2 on the 15 pin I O Connector HARVARD APPARA TUS Select which type of event the step will look for and then press Motor Stall the Enter button green check mark ta accept or press the Advanced Options I O Event Cancel button fred X to return withdraw to the previous screen Link in Selection Screen The following options are available when setting an I O Event Link in command This command requires that you have a method set with the specific actions steps you want to perform if the event occurs so that you can choose the Method name being linked by this command Input High to Low Will link to the desired method upon a logic low input signal falling edge Input Low to High Will link to the desired method upon a logic high input signal rising edge e User Press Will link to the desired method upon a manual input Motor Stall Will link to the desired method upon a Motor Stall event e Withdraw Will
34. is used to define a programmable address that can be used in external software applications that will call each PHD ULTRA pump individually TO MODIFY THE PUMP ADDRESS 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu choose the Pump Address button to enter the Set Pump Address screen 3 Type the desired address value using the onscreen number pad Valid entries are 00 99 4 When you have entered the desired address value choose Accept to save your changes and return to the Settings menu Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 24 Setting the Pump Baud Rate For attaching the PHD ULTRA to an external device using RS 232 or RJ 11 serial communications you can easily adjust the baud rate to optimize communications TO ADJUST THE PUMP BAUD RATE 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu choose the Baud Rate button to enter the Set Baud Rate screen 3 Select the desired preset level value 9600 19200 38400 57600 115200 HARVARD APPARA TUS 04 28 09 11 59 39 AM Select a baud rate and then press the Enter button green check mark ta accept ar press the Cancel button red X to cancel The Set Baud Rate screen your settings and return to the witb tbe 115200 rate value selected previous screen o 115400 4 Whe
35. item or moving the button focus on the touchscreen Selecting an item prepares it for an action and requires a second touch on the touchscreen to confirm the selection For example when you select the Syringe Manufacturer Brand on the Syringe Selection screen it is first highlighted in magenta Pressing the selected choice a second time confirms accepts the selection You can also press the Accept button to confirm the selection We will use the term select to refer to this double press or press confirm action throughout this guide Illustrations Unless otherwise indicated the values in the illustrations of this manual are examples only They are not intended to indicate the exact values you will see or to suggest the values you should use Special Messages Callouts The following special messages and callouts appear throughout the guide to indicate information that requires special attention personal injury or damage to the PHD ULTRA Pump during operation Notes provide helpful instructions that can help you make better use of Warnings or hazard instructions provide information to help you avoid e the PHD ULTRA pump Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 7 General Information WARRANTY AND REPAIR INFORMATION CAUTION Refer to safety information and setting up the PHD ULTRA before plugging in PHD ULTRA pump Manual Description This manual is designed to pr
36. measure ml ul nl or pb as appropriate then choose Accept to confirm your selection 12 When all parameters are correctly set on the Set Target Volume or Time screen choose Accept to confirm your settings and return to the Step Definition screen RAMP PROFILE The Ramp Rate profile is used to create a linearly increasing or decreasing flow rate for a single pumping mode infuse or withdraw over the course of a defined time period Target Time Ramp Rate Pump Operation Profile When creating or editing a Ramp Rate profile you will define three primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw e The Flow Rate at the beginning and end of the pumping cycle e The Target Time that determines the total length of time over which the ramp will be calculated During a Ramp Rate profile the PHD ULTRA varies the flow rate linearly between the starting rate and ending rate over the full time period entered A Ramp Rate profile can be applied to an increasing or decreasing rate application When creating or editing a Ramp Rate profile you can set the rate in ml ul nl or pl units of volume using sec or h m s hours minutes seconds as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse and Withdraw Rate screens Publication
37. moving Steps in a previously created Method DELETING A STEP FROM A METHOD 1 From the Method Main screen choose the Step Definition button to enter the Method Steps Summary screen 2 Highlight select with a single touch the Step that you want to delete 3 Choose the Delete Step button The application will display a warning message in the Message Area asking you to confirm your deletion request HARVARD APPARA TUS 04 28 09 1 22 38 PM 5 Seconds 100 2 nlimin 106 ml rin Delete Method Step 50 ml min showing confirmation message on the screen 4 To confirm the deletion choose the Delete Step button again The Step is removed and you are returned to the Method Steps Summary screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 49 DUPLICATE A STEP IN A METHOD Use this procedure to insert a copy of a Step with all its associated parameter settings into a Method 1 From the Method Main screen choose the Step Definition button to enter the Method Steps Summary screen 2 Highlight select with a single touch the Step that you want to copy 3 Choose the Duplicate Step button The application will insert a copy of the highlighted Step to the bottom of the Method i e the last Step in the Method DEFINING METHOD STEP PARAMETERS This section of the User Guide describes each of the available functions available for use in PHD ULTRA Preloa
38. the export is complete Note that long Methods may take a few seconds to export Before disconnecting the pumps verify that the Method was successfully exported by choosing Method Select from the Method Main screen on the destination pump then browse the available Methods to verify that the exported Method is shown in the menu Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 78 EXPORTING A METHOD TO AN EXTERNAL COMPUTER Follow these instructions to export a Method to an external computer 1 2 ve IL od GA e 10 Connect the pump to the external computer via an RS 232 or USB cable On the computer open the HyperTerminal application and verify that the computer can communicate with the pump On the PHD ULTRA from the Method Main screen choose Method Select then choose the Method you would like to export Choose the File Options button In HyperTerminal choose Transfer Capture Text Enter the File Name that you want to save the Method under then choose Start Choose Export Method to PC The pump will display a confirmation message in the Message Area on the screen when the export is complete Note that long Methods may take a few seconds to export At the destination computer in HyperTerminal when the Method is done transferring the message mend will appear onscreen In HyperTerminal choose Transfer Capture Text Stop Your Meth
39. valve on PC command actuate the valve Load the tubing into the outside slot Tube set is included Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 129 APPENDIX J AUTO FILL VALVES There are two Auto Fill Valve models available Catalog No Product 55 7002 Autofill Valve Box Normal Pressure 30 psi Supplied with 1 8 inch ID 1 4 inch OD silastic tubing and connector to syringe 55 7004 Autofill Valve Box High Pressure 200 psi Supplied with Swagelok fittings 70 4005 Adapter PHD Digital I O Setting Up the Valve Set up consists of three phases Voltage selection 110 or 220V Pump to valve electrical connection and Valve tubing to syringe hook up 1 Power Connection Connect the line cord to the power supply included Attach the power supply to the input jack on the valve box 2 Pump to Valve Electrical Connection The cable fitted with a male 9 pin D sub connector must be connected to the female D sub connector on the rear of the PHD ULTRA Syringe Pump using the Digital I O Adapter Cat No 70 3105 Power can now be switched on with the illuminated power On Off switch on the front valve housing 3 Pump to Valve Tubing Connection Swagelok fitting should be finger tight plus 1 1 4 turn Tubing is 304 stainless steel 1 4 inch O D wall thickness 0 035 inch i a aes Silicon tubing 1 4 inch OD 1 8 inch ID Refill lt j Refill ul e Syringe In
40. 419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual PHD ULTRA Specifications Accuracy Reproducibility Syringes Minimum Maximum Flow Rate Minimum 0 5 pl syringe Maximum 140 ml syringe Display Non Volatile Memory Connectors RS 232 RS 485 USB 1 0 amp TTL Linear Force Max Drive Motor Motor Drive Control Motor Micro Steps per one rev of Lead Screw Step Rate Minimum Maximum Pusher Travel Rate Minimum Maximum Power Dimensions Control Box Remote Box Weight Atmospheric Specifications Operating Temperature Storage Temperature Humidity Mode of Operation Classification Pollution Degree Installation Category Supplier Name Supplier Address Regulatory Certifications Safety Declarations EMC Declaration Stand Alone 0 25 0 05 0 5 ul 140 ml 1 56 pl min 215 8 ml min 4 3 WQVGA TFT Color Display w Touch Screen Stores all settings 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 34 kg 75 Ibs Y 100 Force Selection 0 9 Stepper Motor Controlled Microprocessor with 1 16 microstepping 12 800 27 5 sec ustep 26 usec ustep 0 18 um min 190 80 mm min 100 240 VAC 50 60 Hz 50 W 0 5 A fuse 120x8 5x7 0in 30 5 x21 6x 17 8 cm N A 4 5 kg 10 lbs 4 C to 40 C 40 F to 104 F 10 C to 70 C 14 F to 158 F 20 to 80 RH non condensing Continuous Class 1 Harvard Apparatus 84
41. 5 remote system 1 On the control box connect the RS 485 cable to RS 485 OUT connector 2 On the remote unit connect the RS 485 cable to RS 485 IN connector Legacy Remote System To connect a legacy remote system 1 Connect the male end of the cable to the control box 2 Connect the female end of the cable to the remote unit Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 14 NAVIGATING THE PHD ULTRA EZ PRO SOFTWARE The PHD ULTRA uses a high resolution touchscreen with an icon enabled graphical user interface and an advanced methods architecture to streamline simple to complex tasks The PHD ULTRA Workspace The PHD ULTRA touchscreen interface is divided into three basic areas iai Operations Display this section of the screen is used to display pump operations method information general parameter settings gt and the real time display of data while the pump is running e o o Message Area this section is used to Y gt display the pump name the time date Pd display and the message box which 7 contains operational messages while the HARVARD APPARATUS ns istunning 10 27 09 11 35 03 PM Enter your settings to the left and press the RUN or Run Preview BD plastic 50 ml 26 594 mm button Make sure to select the d appropriate syringe qe Navigation Buttons this area of the touchscreen contains the b
42. 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA TO SET UP EDIT A RAMP RATE PROFILE 1 From the Step Definition screen for a Ramp Rate profile choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation HARVARD APPARA TUS Enter your settings to the left and then press the Enter button Start 0 ml min End 0 ml min green check mark to accept or press the Cancel button red X mn to return to the previous screen Step Definition screen for a Ramp Rate profile O secondis 2 Choose the Set Start amp End Rate button to enter the Set Start amp End Rates screen HARVARD APPARA TUS Start Rate o 04 28 09 1 24 36 PM Enter your rates and units and naa HLE then press the Enter button green check mark to accept or press the Cancel button red X E to cancel your settings and return Set Start and End Rates 105 935 to the previous screen screen ml min 100 205 A v Almin 3 Select the Start Rate button Gt should be selected by default as indicated by the active data entry box to the right of the button Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button 4 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pD and the unit of time sec min hr as appropriate the
43. A Syringe Pump Series User s Manual Using The PHD ULTRA 71 PO EVENT TRIGGERS The PHD ULTRA pump permits easy connection to external I O devices via a variety of interfaces including a built in 15 pin D sub connector The PHD ULTRA software contains simple commands for incorporating I O controls into your pump Methods Integrated commands for control of an external valve multi pump synchronization and general trigger signals are integrated in the software The following procedure describes the use of the Advanced Options I O commands Following the procedure is a description of the selection menu options for each of the I O command types Details for the electrical connection controls and pin out description for the 15 pin User I O Connector are provided in Appendix E of this manual The PHD ULTRA manages trigger events through specific pins on the I O Connector port on the back of the pump The following details define pin logic e Triggers are communicated via Pin 4 or Pin 5 Pump synchronization commands are communicated via Pin 6 e Event signals are communicated via Pin 2 O TRIGGER OUT The I O Trigger out command is used to send a signal to an external device when the Step in the Method is reached HARVARD APPARA TUS Select which trigger port the EH signal will be sent aut af NEM Advanced Options I O Trigger out Selection Screen EI The following options are available when setting an I O Trig
44. D ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 57 TO SET UP EDIT A PULSE FLOW PROFILE 1 10 11 12 13 14 I5 16 L5 18 From the Step Definition screen for a Pulse Rate Flow choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation Choose the Set Rates button to enter the Set Pulse Rates screen Select the Rate 1 button it should be selected by default as indicated by the active data entry box to the right of the button Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections Repeat Steps 3 to 5 for the second flow rate by selecting the Rate 2 button and continuing data entry When all parameters are correctly set on the Set Pulse Rates screen choose Accept to confirm your settings and return to Step Definition screen Select the Volume or Time button to determine how the pump cycle length will be managed Volume calculates each cycle stage by pumping for the length of time needed to pump the specified volume Time manages each cycle stage by pumping for the amount of time specified Depending on whether you have selected the Volume or Time button in
45. Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA REPEAT STEP The Repeat Command is used to repeat Steps in a procedure a defined number of times To use this Command you will tell the application what Steps you want to Repeat and how many times you want them repeated The Repeat Step command creates a jump back to a specific Step in a Method that executes the defined Step plus all following Steps up to the Repeat command i e it creates a Method loop When defining a Repeat jump in a Method the Command targets the Step sequence position and not the function that currently occupies that position For example if you create a Repeat jump to Step 4 in a Method currently a Bolus Pump Operation Profile and subsequently move a Constant Rate profile into the Step 4 position the Repeat jump will then connect to the Constant Rate operation and not to the Bolus operation TO INSERT A STEP REPEAT SEQUENCE IN A METHOD From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the repeat step to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen Choose Repeat to insert the Repeat Step function into the Method p S A qw ee Select the Step s you would like to repeat from by selecting the Step button twice or selecting onc
46. HyperTerminal is a Windows application designed to support the external control of devices such as the PHD ULTRA pump through an RS 232 or USB connection The following instructions describe the configuration of the HyperTerminal application 1 Select Start All Programs Accessories Communications HyperTerminal Connection Description lt PHD_Ultra_Cornrl 2 Enter a name for a New Connection i e PHD Ultra Comm then click OK Connect To 3 Select the Virtual Comm Port from the Connect using drop down list Click OK If the Virtual Comm Port is not known use Device Manager to find it Instructions on setting up the Virtual CommPort Driver are supplied in Appendix E COM8 Properties 115200 v 4 Setup the Port Settings as shown above and click OK Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 80 PHD_Ultra_Comm Properties 5 Verify the Settings are as shown above ASCII Setup 6 Choose ASCII Setup and select Echo typed characters locally then click OK to complete the setup Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 81 PUMP CHAIN COMMANDS The Pump Chain commands allow all pump control information to be managed from an external computer source These commands can also be used to control a series of pumps u
47. LE AUDIBLE ALARMS 1 Inthe Audible Alarms menu select the specific alarm or alarms that you wish to set On the Settings screen select Touch Screen Lock Enable Note that a checkbox on the button will be checked to indicate that you have selected an option but the button will not be displayed in magenta as with other select options in the software 2 If you select the Near End of Run Alarm choose the Percentage Set button to access the Near End of Run Select menu that is used to define at what point in the Method the alarm will sound Select a preset value 75 80 85 90 95 or choose Set Other Value and Enter the desired Percent Done value using the keypad If you choose Set Other Value choose the Accept button when finished When you have made your selection on the Near End of Run Select screen choose the Accept button to complete your changes HARVARD APPARA TUS 04 28 09 11 46 29 AM Select an existing percentage or select set other value and then press the Enter button green Set Audible Alarms Near check mark to accept or press End of Run Select screen pe dede DC do el and tbe Set Other Value button Set Other Value E Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Locking the Touchscreen Setting Up The PHD ULTRA 20 A touchscreen lock function can be enabled to prevent accidental operation or interruption of operation Unlocking the touchscreen requires a
48. Link Method to the previous screen Command selection screen TO LINK TO A STORED METHOD From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the Link Command to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen Choose Link to enter the Link Selection screen and display a list of the saved Methods available a MP dee Sow dee Ge Double tap the Method that you want to link to in the current Method If the desired Method is not listed on the screen use the Scroll Up Scroll Down or Page Up Page Down buttons to scroll through the Method List Upon selection you are returned to the Method Steps Summary screen STOP The Stop Command is used to instruct the pump to stop operation This Command can be used in conjunction with I O Events to control pump operation based on external events TO INSERT A STOP COMMAND IN A METHOD From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the Stop Command to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen pt Ie M d es Choose Stop to insert the Stop Command into the Method Publication 5419 002 Rev E Harvard Apparatus PHD ULTR
49. Message The Message Area on every screen provides a summary of what you are supposed to do and many screens offer warnings or cautions if you do something wrong 8 Set Your Syringe One of the most basic selections on the system is choosing the syringe that is loaded This setting is persistent across all Quick Start Methods the ones we loaded to help you get started fast That means it will be the default syringe choice in every Quick Start Method once you set it up However User Defined Methods save their own syringe settings If you are creating a New Method the default syringe will be the syringe selected for Quick Start Methods 9 Get Connected If you are using the PHD Ultra in conjunction with external devices ones you want to trigger or ones that you will respond to there are commands available to simplify the setup of these connections The Advanced Options in the Method Steps Profiles section of this manual describe these functions 10 Manual Pumping Buttons for manual control of the pump for fast infusion or fast withdrawal action are available on many screens in the application Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual General Information General Information CONVENTIONS USED IN THIS GUIDE This guide describes basic procedures for operating the PHD ULTRA Syringe Pump including both hardware operations and software operations accessed through the pump s touchscr
50. PHD ULTRA Syringe Pump Series User s Guide PHD ULTRA Series MAI 70 3xxx PATENTS PENDING HARVARD APPARATUS Publication 5419 002 Rev E Table of Contents 2 Table of Contents QUICK GUIAS risa iia 6 10 Tiiges You Need To now To Use The PHD ULTRA aora 6 General Information T P 7 Conventions Used Tri Pis dE its 7 Warran ana Repair TIO AOU ias 8 MUELA HEB ie AA A E o aint A A E E esata an 8 b ccis FT A 8 Repar Races ana ARES n 8 o A T A E 8 CDE OE eaa E PO A 8 S EE Ea ino a Marne mee OPS pere 9 Introduction To The PHD ULTRA Pump creer ese rr cansar ranenranearanranss 10 Product Overview Theoy ol Cet AO sss et ideo oeste 10 AA bt ree ie on A 10 DeLee Sei ales olan NE UE Um 11 Setting Up The PHD ULTRA With EZ PRO ccssscssessessesssseseessessenseeensesseseenees 12 PTS Me C NER OO TO 12 Power Connecions and Pnp Sari Dereser laneros 13 Remote Pump Parar CONE CONS MT arn erase 15 Nica The PHD CETRA EZ PRO SO lat n aang 14 Tee PHD DEN Works ro Nc 14 Selecting An Ttem From A Menu List rss ii 15 lioro Waa 6 S52 GE a EN N A EAA AEE R E 16 EZ PRO Touch Screen Button Reference miesies nii eE b reris adeat inda 17 SAA A A o ea ER Eara 18 Sete tie Mole T DEDOS pst a 18 Locas The Toluca SOrschl ansiada ere da caiste die rT 20 Seno ESI AE a E Oo ne
51. RJ 11 Standard Pin 1 to Pin 1 Telephone Cable Cat 55 7760 or 72 2478 RJ 11 PUMP IN ADDRn RJ 11 PUMP IN ADDR 1 Non Standard Telephone Cable Cat 55 7760 or 72 2478 NOTE Pumps 1 n also have RJ 11 telephone jacks NOTE When daisy chaining with RS 232 each pump must be self powered If a pump in the RS 232 daisy chain is powered down all pumps down stream from it will lose Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 114 PUMP TO PUMP CONNECTION RS 485 CAUTION Do not connect to firewire ports on a PC Damage may ocur to pump and or PC Mating Face RS 485 In Signal RS 485 Out Signal Notes PWR IN PWR OUT 430V Protected by resettable fuse GND GND RS 485 A TIED TO PIN 4 100 Q Ye w to RS 485 B TIED TO PIN 3 NEE ANOR TIED TO PIN 6 RS 485 A TIED TO PIN 5 RS 485 B RS 85 In Out IEEE 1394 Sockets USBor USBor PUMP RS 485 RS 485 PUMP RS 485 RS 485 PUMP IN RS 232 D subg ADDR OUT eres IN ADDR1 UP EEE1394 ADDEN D Sub 9 Cable Cable F F Cable RS 232 IN Daisy chaining via RS 485 NOTE When daisy chaining with RS 485 at minimum every fourth pump must be self powered i e another stand alone unit This can be affected by Force Selection X NOTE When applying power to pumps in a daisy chain apply power to the last group and work backwards hs NOTE When removing power be sure to remember that power r
52. SS Sets or displays the pump address Valid range is O to 99 Command format address 0 99 Query response It Pump address is lt cr gt It lt prompt gt Notes Pumps with an address of O are masters and pumps with an address between 1 and 99 are slaves BAUD Sets or displays the RS232 port baud rate Valid baud rates are 9600 19200 38400 57600 115200 128000 230400 256000 460800 and 921600 Note that some computers may not be able to handle baud rates above 115200 Command format baud 9600 19200 38400 57600 115200 128000 230400 256000 460800 921600 Query response lt lf gt baud lt cr gt Ift prompt Notes If this command is entered via RS232 the prompt is displayed at the previous baud rate and then the baud rate is changed CATALOG Displays a catalog of methods stored in the pump Command format cat Query response If Method name Size lt cr gt lt lf gt z Cr If method name lt cr gt This line is repeated for each file on the disk It 1f file s using steps lt cr gt It prompt Notes The file size is the number of steps in the method plus one for the file information header The last line shows the number of files and the total number of steps being used on the disk DELMETHOD Deletes a method stored in the pump Command format delmethod method
53. TRATION PROFILE The Concentration Delivery profile is used to manage infusion of a defined concentration of drug or other liquid to a set dosage level based on the subject s weight Animal Weight Drug Concentration Doses Time Concentration Deliver Between y Pump Operation Profile Doses Dose Time When creating or editing a Concentration Delivery profile you will define the following operating parameters e The Weight of the target subject e The Flow Rate to be used during infusion e The concentration of the drug or other liquid being infused e Either the target dosage or the target infusion time the pump automatically calculates whichever value you do not enter e If you wish to infuse multiple doses to the target you can specify the number of doses along with a delay between dosings Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 60 During a Concentration profile the PHD ULTRA operates at a constant infusion rate and automatically stops when the target dosage rate is reached When creating or editing a Concentration flow profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Rate and Concentr
54. ULTRA 57 9 To start a pump Method choose the Run button While a Method is running the Pump Run LED will be lit The Run Method Run screen will provide real time feedback on the progress of the Method operation as shown below Quick Start Method Preloaded User Defined Methods Current flow direction Total time elapsed for Method infuse withdraw Volume withdrawn infused and volume remaining Total Volume Infused across all Method steps Previous Step Information Time elapsed Next Step Information Time remaining Total volume infused HARVARD APPARA TUS change Current settings 08 94 09 7 30 12 AM Infuse Withdraw HA Steel 28 60 mm 50 00 ml Infusion started Press the Target 50 ml Change Current Settings box to Infuse 122 578 ml min change your settings or press the withdraw 122 578 ml min red Stop button to stop the Flow pump Direction A I z 20 384 ol 00 00 10 Time Elapsed Time Remaining 00 00 38 Total Inf Volume 19 884 ml e 4 O Force Level 100 HARVARD APPARATUS 3 stepis Force Level 100 08 24 09 7 33 34 AM Method started Press the red BD plastic 50 ml 26 594 mm sion button ta ston the pam Constant Rate Rate 50 ml min Tm Target 5 ml Time Elapsed 00 00 10 Total Volume Dispensed 1 087 ml Quick Start Method Run screen after a Metbod bas been started User Defined Metbod Run Screen after a method bas been started Publication 5419 002 Rev E H
55. Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 5 When all parameters are correctly set on the Set Infuse Rate screen choose Accept to confirm your settings and return to the Quick Start or Method Main screen TARGET VOLUME TIME SELECT The Target Volume Time Select function is used to control how long the PHD ULTRA will operate at the set flow rate The run time can be controlled by either pumping to a total volume pumped or the total length of time pumped Quick Start single function Methods Infuse Only or Withdraw Only permit controlling the pump for either target time or volume Quick Start composite Methods Infuse Withdraw or Withdraw Infuse only permit control by Target Volume When setting this parameter you can choose between the following units of volume ml ul nl or pl and the following units of time sec h min sec The system automatically calculates a Maximum Volume value based on the syringe selected or custom defined for the Method HARVARD APPARA TUS Volume 04 28 09 1 10 59 PM e 5 ED Enter your target volume ar time Sing and then press the Enter button green check mark to accept or Set Target Volume Time for press the Cancel button red X ta cancel your settings and return setting pumping duration to the previous screen in a Quick Start Infuse Only Metbod
56. Withdraw Rate screens NOTE The following instructions describe setting the Infuse Rate The instructions are BZ the same for Withdraw Rate On an Infuse Withdraw combination Method you can set both rates on a single screen by toggling between the two rate options using the Infuse Rate or Withdraw Rate buttons at the top of the screen Infuse Rate Infuse Rate Withdraw Rate nl min Publication 5419 002 Rev E HARVARD APPARATUS 04 28 09 1 08 54 PM Enter your flow rate and units and then press the Enter buttan green check mark ta accept ar press the Cancel button red X to cancel your settings and return to the previous screen HARVARD APPARA TUS 04 28 09 1 09 37 PM Enter your flow rate and units and then press the Enter button green check mark to accept or press the Cancel button red X ta cancel your settings and return to the previous screen Set Infuse Rate screen in a Quick Start Infuse Only Metbod Set Infuse and Witbdraw Rates in a Quick Start Infuse Witbdraw combined Metbod Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 41 TO SET THE INFUSE RATE INFUSE ONLY METHOD 1 From the Quick Start or Method Main screen choose the Infuse Rate Select button 2 Onthe Set Infuse Rate screen enter the desired rate value using the keypad or select the Minimum or Maximum Rate button 3 Ifthe Units of Rate are not correct for your application choose the
57. Z PRO User Defined Methods User Defined Methods are created by the user through the application To create a custom Pump Control Method you select New Method After naming the method select the syringe then define the infuse or withdraw operations desired using the available Pump Operation Profiles User Defined Methods can be stored on the PHD ULTRA up to the maximum memory capacity of approximately 800 program steps programmable model only The user can manage the library of stored Methods using convenient touchscreen controls Y NOTE Method Header takes up 1 step of memory Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 31 RUNNING THE PUMP Controlling The Pump The software provides Fast Forward Infuse and Fast Reverse Withdraw buttons that can be used to manually control the Pusher block and either Withdraw contents into the syringe or Infuse contents from the syringe Use this button to advance the Pusher Use this button to advance the Pusher bar in the reverse withdraw direction Bar in the forward infuse direction NOTE Operating the Fast Forward and Fast Reverse will not update the Volume or Time counters in the Method Run screens Basic Method Operations The following operations are common across all Method types VIEWING CURRENT METHOD PARAMETERS As described later in this Guide PHD ULTRA pump operation is controlled by Meth
58. all of the advanced programming tools available on the PHD ULTRA this model also allows users to create and store multiple user defined Methods on the pump NOTE This manual has been written to accommodate Infuse Withdraw Programmable e configurations Where features capabilities are not available in the Infuse Only or Infuse Withdraw basic models we have identified that in the manual PUMP CONFIGURATIONS The PHD ULTRA Syringe Pump is available in the following configurations e Standard Pump This configuration is the most common It consists of a single unit with the syringe pumping mechanism attached to the top of the control box e Remote Pump This configuration is a two piece unit with the syringe pumping mechanism and the control box separated by a 30 foot cable Two meter one meter and 0 5 m cables are available for purchase separately for RS 485 remotes A 6 foot cable is available for the Legacy remotes e Push Pull Pump This configuration consists of a single unit with the syringe pumping mechanism attached to the top of the control box The pump can hold up to four syringes 2 on either side of the pusher block The pump can simultaneously infuse and withdraw the exact same amount e Satellite Pump Units Satellite units consist of a syringe pumping mechanism and control electronics but do not have a user interface They are powered and controlled from a master stand alone PHD ULTRA via the RS 485 cable Publication 5
59. are v1 3 1 amp Version Number RTOS Freek TOS v6 0 1 Pump address O Disk space free 790 method steps 9 Verify that the version number has been updated in the Power Up screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 122 PHD ULTRA Display Error Messages Motor Stalled This indicates that the pusher block travel has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing occlusion syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and or increase the force setting then press RUN The pump will continue the interrupted procedure where it stopped Out of Range A value was entered or encountered in a pump program that was beyond the pump s limits Power Failure Notification If power to the pump is interrupted during operation an alarm will sound to alert the user to the interruption When power is restored an onscreen message is displayed to indicate the interruption HARVARD APPARATUS 10 2709 12 03 41 PM Quick Start Infuse Only HA rca 100 ml 34 9 mm The PHD ULTRA Pump will notify you of a power failure 182 529 k min Sail EHI lp Low Battery Indication Time Date area will turn red when battery is low See page 119 for instructions on changing the battery
60. arvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 38 10 To Pause a Method during operation choose the Method Stop button HARVARD APPARATUS 3 step s Force Level 100 08 24 09 7 35 59 AM BD plastic 50 ml 26 594 mm Method paused Press the AER Run button to resume pumping Delay press the Clear button orange Method Run screen with a TN TM OR arrow to reset or press the Back j Delay 5 secs button to return to the previous User Defined Method paused screen on Step 2 Note the currently active Step is bigbligbted Constant Rate Rate SO ml min Target 5 ml Time Elapsed 00 00 10 Total Volume Dispensed 1 021 ml 11 To Resume a Method that is paused choose the Run button 12 To Cancel a Method that is paused choose the Clear button The Method is reset to its initial parameters when Clear is pressed 13 Upon completion of a Method operation choose the Re Run button to clear all counters and timers and repeat the Method Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 39 USING AN EZ PRO QUICK START METHOD Quick Start Methods are designed to help you quickty and conveniently use the PHD ULTRA for simple infusion or withdrawal applications Each Quick Start Method requires a minimal number of user entries to operate Basic Operation The following flow diagram describes the use of a Qui
61. ation screen The ingredient concentration is entered in mg ml HARVARD APPARA TUS 04 28 09 1 29 16 PM Enter your information and then press the Enter button green check mark to accept or press the Cancel button red X to Step Definition screen for a E cancel your settings and return to Concentration Deliver Set Dose or Duration the previous screen Y O imagikg O second s profile D grams a marl 0 Cose s O second s TO SET UP EDIT A CONCENTRATION DELIVERY PROFILE 1 From the Step Definition screen for a Concentration profile choose the Animal Weight button to enter the Set Animal Weight screen HARVARD APPARA TUS Weight grams o o 04 28 09 1 31 43 PM Enter the animal weight and then press the Enter button green check mark ta accept ar press E the Cancel button red to cancel your settings and return to Set Animal weight screen in El E 6 the previous screen Concentration profile 2 Enter the Weight in grams then choose the Accept button to return to the Step Definition screen 3 Choose the Set Rate and Concentration button to enter the Set Rate and Concentration screen HARVARD APPARA TUS Infuse Rate jse 04 28 09 1 34 16 PM Enter your information and then press the Enter button green check mark to accept or press the Cancel button red X to Set Rate and Concentration cancel your settings and return to screen in Concentration profile 105 985 the previous screen
62. b 2 Unscrew and remove the Retaining Nut and slide the clamp bar off the Syringe Barrel Clamp Knob 3 Flip the bracket over reassemble the Retaining Nut and screw the assembly back onto the pump Large diameter syringe clamping Large vs small diameter syringe clamping Small diameter syringe clamping Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 29 Using The PHD ULTRA PUMP OPERATION OVERVIEW The following diagram provides an overview of basic operation for the PHD ULTRA STEP 1 Select A Method Choose an operating Method which defines the syringe being used the direction infuse or withdraw and rate of flow as well as any dynamic control of external devices If one does not exist you will create a new Method STEP 2 Enter Operating Parameters Enter the operating parameters for each step in your method including specifics about the syringe syringes being used the type of flow operations you want to use that rate of flow and any details that define the pumping operation STEP 3 Preview Your Method Check all settings using the Method preview function Change any required parameters as necessary STEP 4 Run Your Method Method Monitor real time status detailing the progress of the Method OVERVIEW OF METHODS To operate the PHD ULTRA you will define all the required parameters for withdrawing or infusing liquids through a Pu
63. cept HARVARD APPARA TUS 10 27 09 11 55 37 AM Enter your total time and number of steps and then press the Enter button green check mark to accept or press the Cancel button Gradi adient Time and Type fred X to cancel and return to ve the previous screen Entry Screen NOTE For a Constant rate E or Ramp Va profile choose O steps For a Stepped profile Ll select the number of steps desired NOTE When running a gradient step ensure that no unused pumps are included in e the pump chain Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 65 AUTOFILL CYCLING PROFILE The Autofill Cycling Profile is an automated Infuse Withdraw or Withdraw Infuse step that operates at constant but separately defined flow rates for the withdraw and infuse operations The Autofill profile is designed to work in conjunction with an autofill valve that controls the switching of flows from a reservoir for refilling the syringe to the target infusion subject NOTE Pin 8 of the 15 pin I O Connector adapter on the rear of the PHD ULTRA will p actuate to control a Harvard Apparatus Valve Box used in conjunction with an Autofill Pump Operation Profile Cycles or Autofill Cycling Pump Operation Profile Total Volume Withdraw Time Volume When creating or editing an Autofill Cycling profile you will define the following operating parameters e The Flow M
64. ck Start Method Select Method Type Select Syringe Model OR Create New Syringe Type Quick Start Method operation flow diagram Enter Flow Rate Parameters Infuse Only Withdraw Only or Infuse and Withdraw Enter Target Volume or Time All the required parameter options are accessed from the Quick Start screen The following sections provide a detailed overview of operation for a Quick Start Method STEP BY STEP OPERATION EXAMPLE QUICK START INFUSE WITHDRAW METHOD The following procedure describes operation using the Quick Start Infuse Withdraw Method HARVARD APPARA TUS 10 28 09 11 45 17 AM Enter your settings ta the left and then press the green Run or BD plastic 50 ml 26 594 mm Run Preview button when finished Make sure to set a target volume Quick Start Infuse Withcdraw Infuse Witbdraw Quick Start Metbod screen EA EPA 1 From the Quick Start screen the Infuse Withdraw Quick Start Method should be displayed in the Method Select button If that Method is not displayed select the correct Method using the procedure outlined in Running The Pump 2 Choose the Syringe Select button to enter the Syringe Selection screen 3 Follow the instructions in the Syringe Selection section of this Guide to complete the selection of a syringe from the built in Syringe Lookup Table or to create a new syringe When finished choose the Accept button to save your changes or choose the Cancel button to cancel
65. ck to return to the Method Selection screen Renaming A Method Follow these instructions to rename a previously saved Method 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want to rename then choose the File Options button to enter the File Options screen 3 Choose Rename Method to access the Method Name Entry screen The current Method Name will be displayed in the text entry box 4 On the Method Name screen type the new name for your Method using the onscreen keyboard Choose the Symbols Numbers button 123 to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters mode choose the Letters button ABC 5 When finished entering the new Method Name choose Accept to save your changes and return to the File Options screen 6 Choose Back to return to the Method Selection screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 76 Appending A Method Follow these instructions to Append a previously created Method into a new Method 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want as the base Method
66. d format ivolume Query response If xl lt cr gt It prompt SVOLUME Sets or displays syringe volume Quick Start mode only Command format svolume TVOLUME Sets or displays the target volume Quick Start mode only Command format tvolume target volume volume units Query response If 2 Target volume not set lt cr gt It prompt Of If xl xxx lt cr gt It prompt WVOLUME Displays the withdrawn volume Quick Start mode only Command format wvolume Query response It xl lt cr gt Ift prompt TIME COMMANDS CITIME Clears the infused time Quick Start mode only Command format citime Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 90 TIME COMMANDS CONTINUED CTIME Clears both the infused and withdrawn times Quick Start mode only Command format ctime CTTIME Clears the target time Quick Start mode only Command format cttime CWTIME Clears the withdrawn time Quick Start mode only Command format cwtime ITIME Displays the infused time Quick Start mode only Command format itime Query response If seconds lt cr gt It prompt Of If cr It prompt TTIME Sets or displays the target time Quick Start mode only Command format ttime target time Query respo
67. ded and User Defined Methods The information in this section is designed to help you set the individual parameter values in each of the Method Step options and to properly use the Advanced Options commands The information that follows is organized into two sections e Pump Operation Profiles e Advanced Options Commands and I O Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Pump Operation Profiles Using The PHD ULTRA 50 The PHD ULTRA Preloaded Methods and all User Defined Methods use Pump Operation Profiles as part of the Methods controlling the pump These profiles are combined with a syringe selection and any Commands or Input Output triggers to create a complete Method The following sections describe each profile along with instructions for setting up the profile The following Pump Operation Profiles are available on the PHD ULTRA CONSTANT RATE Time Volume STEPPED RATE Time Volume BOLUS DELIVERY Time Volume GRADIENT RAMP RATE PULSE FLOW Pulses Time Volume CONCENTRATION DELIVERY AUTOFILL Infuse Flow Rate Withdraw Pump Operation Profiles available on the PHD ULTRA Publication 5419 002 Rev E Animal Weight Drug Concentraton Doses Time Lag Dose Time Cycles or Total Volume Time Volume Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 51 CONSTANT
68. draw 15 898 ml min return to the previous screen un screen Showing Lol T Gang 4 syringes gt BEE Time Elapsed 00 00 00 A IIS Force Level 100 NOTE If syringe Gang number 1 the value will not appear on any screen Only displays for Gang 2 Controlling The Pump Using A Stored Method The PHD ULTRA Methods contain the detailed parameters required to control the pump for infuse or withdraw operation This section describes the basic operation of the pump using a stored Method SELECTING A METHOD 1 From either the Quick Start or the Method Main screen choose Method Select to access the Method Selection screen HARVARD APPARATUS 10 27 09 10 08 05 AM Select an existing method or uickStart E enel Hei E Aen ane Method Selection screen QuickStart check mark showing a listing of Methods in an Infusion Witbdrawal QuickStart Programmable model PHD ULTRA pump QuickStart Method Method From the list of available Methods double tap the desired Method If the Method is not displayed in the list you can use the Scroll Up Down buttons or Page Up Down buttons to advance the list up down as needed Be sure to confirm your selection by pressing the button containing the Method name a second time or choosing Accept You will be returned to the Quick Start or Method Main screen with the Method Select button now displaying the desired Method CONFIRMING METHOD PARAMETERS D With the desired Method dis
69. ds 1 master pump plus 2 satellites Description Upgrade Infuse Only to I W t Upgrade Infuse Only to Programmable t Upgrade I W to Programmable t Requires Return to Factory T Not available on satellite boxes For additional items such as Syringe Heaters Spill Sensors Temperature Controllers Nano Fluidic Accessories and much more please visit our website www harvardapparatus com or call 800 272 2775 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Declaration of Conformity Manufacturer Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 U S A Phone 508 893 8999 We herewith declare that the following product Product Name PHD ULTRA Syringe Pump Series Model No PHD ULTRA Catalog 70 3xxx To which this declaration relates is in conformity with the applicable EC Directives harmonized standards and other normative requirements Application of Council Directive s 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive 2002 95 EC RoHS Directive Standard s to which conformity is declared Safety EN 61010 1 2001 2nd Edition Emmissions Immunity EN 61326 2006 05 01 EN 61000 4 2 1995 A1 1998 A2 2001 EN 61000 4 3 2002 EN 61000 4 4 2004 EN 61000 4 5 1995 A1 2001 EN 61000 4 6 2007 EN 61000 4 8 1993 A1 2001 EN 61000 4 11 2004 EN 61000 3 2 2000 EN 61000 3 3 1995 A1 2001 CISPR11 2003 A1 2004
70. e and then choosing Accept HARVARD APPARA TUS 05 01 09 1 29 35 PM te E Select the desired step ta repeat 182 5 ml min fram and then press the Enter button green check mark to Advanced Options Repeat 100 ml min accept or press the Cancel button Step screen Choosing Repeat fred X to cancel your settings brings you to a listing of the available steps in the Method and return to the previous Repeat 7 Enter the number of repeats using the keypad then choose the Accept button to save your changes and return to the Method Steps Summary screen This command creates a loopback to the designated Step in a Method executing the selected Step plus all subsequent Steps up to the point of insertion of the Repeat command For example in the above figure if the Repeat command is set to repeat Step 2 one time the system will loop back to Step 2 and execute both Steps 2 and 3 one additional time Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 69 Using The PHD ULTRA 70 LINK METHOD The Link Command is used to insert a link to another stored Method into the operating sequence of a Method You can Link a Method any place in the Method sequence HARVARD APPARA TUS 04 28 09 1 42 33 PM Select the method to link to and Method then press the Enter button green check mark to accept or Method press the Cancel button red X ta cancel your settings and return Advanced Options
71. e the Method Step that you want the I O Trigger Out to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Select Advanced Options screen Choose the I O Trigger out button to enter the Trigger Step Definition Screen Set your Trigger settings by choosing the appropriate option from the following sub procedures a To send a Trigger signal to an external device via Pin 4 select the Trigger 1 button To send a Trigger signal via Pin 5 select the Trigger 2 button Then select whether the signal will be High or Low by selecting the appropriate button b To send a trigger signal to an external valve select Valve Then select whether the trigger will actuate On button or close Off button by selecting the appropriate button This overrides the Valve Auto setting which means that the valve is controlled by a direction change The valve will default back to auto after leaving the Method Run screen c To send a synchronization signal to a connected pump select Synchronize Then select whether you want to start an infusion action Start Infuse button or a withdraw action Start Withdraw button by selecting the appropriate button Choose the Accept button to save your changes and return to the Method Steps Summary screen NOTE When using triggers the first step in your method should define the trigger e starting level High or Low You may then use the trigger command t
72. e Set Rate button to enter the Set Rate screen 3 Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button 4 If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 6 When all parameters are correctly set on the Set Rate screen choose Accept to confirm your settings and return to Step Definition screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 52 Choose the Set Target Volume or Time button to enter the Set Volume or Time screen On the Set Volume or Time screen select the Volume or Time button as desired Enter the desired value using the keypad or select the Maximum Volume button 10 If the Units of Volume Time are not correct for your application choose the Units button to the right of the parameter entry box For the Units of Volume this choice takes you to the Units Selection screen For the Units of Time option this choice will toggle the button between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 11 If you are changing the Units of Volume on the Units Selection screen select the unit of
73. e Units button to enter the Units selection screen Select the unit of measure button ml ul nl pD appropriate for your application then choose the Accept button to return to the Set Target Volumes screen When all parameters on the Set Volume per Cycle screen are correct choose the Accept button to return to the Step Definition screen Choose the Set Total Volume or Cycles button to enter the Set Total Volume or Cycles screen Select either Total Volume or Cycles to define how the pump will control pumping activity Total Volume will cycle the pump the correct number of times to deliver the total volume according to the Volume per Cycle setting For example if you set a 10 ml Volume per Cycle and set 100 ml in Total Volume the pump will cycle 10 times If you select Total Volume enter the volume desired using the keypad If you select Cycles enter the total number of Infuse Withdraw or Withdraw Infuse cycles that you wish to perform If the Units of Measure associated with Volume are not correct for your application choose the Units button to enter the Units selection screen Select the unit of measure button ml ul nl pD appropriate for your application then choose the Accept button to return to the Set Target Volumes screen When all parameters on the Set Total Volume or Cycles screen are correct choose the Accept button to return to the Step Definition screen NOTE Using maximum rate may not be appropriate during th
74. e Withdraw stage of an Autofill profile since air can be drawn into the syringe or a high vacuum pressure can be produced depending on the syringe and or tubing being used or the fluid viscosity Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 61 Incorporating Advanced Options Commands and I O Into A Method PHD ULTRA Methods can incorporate a variety of commands that expand the versatility of the system by permitting convenient looping chaining of events sending signals to external devices or accepting inbound signals from external devices These Advanced Options controls are described in this section of the Guide To access the Advanced Options choose Advanced Options from the Select Profile screen HARVARD APPARA TUS Delay 04 28 09 1 39 16 PM Select desired function or press O out sbowing available command Trigger options the Cancel button red X to Repeat C Link co return to the previous screen Method Step Profile Selection Advanced Options screen 1 0 in Event GO TO STEP The Go To Command is used to instruct the PHD ULTRA to jump to a particular Step in a Method This can be used to help you loop back or jump forward in a Method protocol and can be used in conjunction with I O Events to make the pump respond to a trigger from an external device When defining a Go To jump in a Method the Command targets the Step sequ
75. e available in 2 5 8 20 50 100 and 200 ml sizes with removable replacement tips The 20 to 200 ml stainless steel syringes offer SWAGELOK fittings in 1 16 1 8 and 1 4 inch sizes A luer lock fitting is also available The tips are interchangeable for 20 to 200 ml syringes The 2 5 ml is available with a 1 16 inch SWAGELOK tip only The 8 ml offers SWAGELOK fittings in 1 16 or 1 8 inch sizes All stainless steel syringes are constructed entirely of 316 stainless steel The 20 to 200 ml stainless steel syringes come standard with two Viton O rings for the barrel and one for the tip These Viton O ring seals insures against leakage These syringes are guaranteed to be leak free for pressures up to 750 psi The 2 5 ml stainless steel syringe contains one Perfluoroelastomer O ring seal on the tip and one Bal Seal on the barrel The 8 ml stainless steel syringe contains two Perfluoroelastomer O ring seals and two Teflon O Ring seals on the barrel and a Perfluoroelastomers O ring on the tip These syringes were designed to utilize the high forces available in our syringe pumps to produce pressures up to 7500 psi and 1500 psi respectively All syringes are supplied with inside diameter dimensions for use with Harvard microprocessor controlled pumps and rate charts for use with older classic pumps Replacement Viton O rings are available as are more chemically resistant Perfluoroelastomer O rings High Pressure Stainless Steel Syring
76. e wizard Appendices Browse for the proper driver installation file and then click next It can be found on the CD supplied with the PHD ULTRA Pump Windows XP or Vista PHDUltra XPVista inf Windows 2000 If a warning message regarding Windows Logo testing is displayed choose Continue Anyway to proceed with the installation The Virtual Commport software will be installed When the software installation is complete click Finish to close the wizard Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 111 Appendices 112 RS 232 Specifications RS 232 9 Pin D Sub Male Baud Rates 9600 19200 38400 57600 115200 Word size 8 Parity None Stop Bits 2 RS 232 Specifications Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 113 Legacy RJ 11 Option Transmit Ground Receive Transmit Ground Pumps with Legacy RJ 11 Option D Sub 9 F F Cable Cat 70 4004 OR D Sub 9 F to Telephone Cable Cat 70 2022 PUMP RJ ADDR OUT RS 232 IN D Sub 9 Non Standard Pin 1 to Pin 1 Telephone Cable Cat 55 7760 or 72 2478 PUMP IN ADDR Y RJ 11 OUT RJ 11 Non Standard Telephone Cable Cat 55 7760 or 72 2478 Daisy Chaining with Legacy RJ 11 Option Y y communications Publication 5419 002 Rev E RJ 11 IN ADDR1 Nan IN Receive PUMP ADDRn PUMP
77. eck mark ar press the Cancel button fred X to return to the previous screen Syringe Dimension Entry screen Enter the diameter of your syringe using the keypad Choose the Volume button and enter the total volume of your syringe If the Volume unit of measure is not correct choose the Unit of Measure button E b que On the Units Selections screen select the Units button appropriate to your syringe then choose Accept You are automatically returned to the Syringe Dimension Entry screen 8 When all parameter entries are correct choose the Accept button to return to the Quick Start or Method Main screen NOTE Each custom method is capable of using and storing a different custom syringe but these custom syringe selections are not saved anywhere that can be recalled A CAUTION Be sure to lower the force value when using glass syringes CAUTION See Setting Force Limits section if using Master Satellite configuration Gang Feature The Gang feature allows multiple syringes to be combined into one common output Multiple syringes combined into a common output 1 From the Configuration screen choose syringe select Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Syringe Stainless Steel 2 3 Enter the number of syringes being used then press the Accept button Quick Start Infuse Withdraw hang 4 syringes Custom 10 3 mm 3219 35 ul min pe
78. ection for DC idi Source recommended mate connector and cable eturn Recommended connection circuit for analog control of motor speed Connect cicuit as shown in the above figure Turning the Potentiometer CW will increase the motor speed pin 4 is 10 VDC and turning the Potentiometer CCW will decrease the motor speed pin 4 is 0 VDC 2 NOTE Refer to the accessories section for recommended mate connector and cable p NOTE Not available on satellite units Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual USB Mating Face 1 5V Data 2 3 Data 4 GND USB VIRTUAL COMMPORT DRIVER INSTALLATION Appendices USB Type B Female When you connect the PHD Ultra pump to a computer via USB Windows will seek to install a driver for communication The following section details the installation of the Virtual CommPort Driver supplied with the PHD Ultra pump 1 A Publication 5419 002 Rev E Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Can Windows connect to Windows Update to search for software O Yes this time only Yes now and every time connect a device No not this time Click Next to continue On connection of the pump to the c
79. ee 121213 4 HARVARD APPARA TUS 10 27 09 11 30 44 AM Select an existing syringe manufacturer or select custom syringe and then press Enter green check mark to accept or press the Cancel button red X to return to the previous screen HARVARD APPARA TUS 10 27 09 11 31 05 AM Enter a value between 1 and 10 and then press the Enter button green check mark to accept or press the Cancel button red X ta cancel your settings and return ta the previous screen HARVARD APPARA TUS 10 27 09 11 31 51 AM Enter your settings to the left and then press the green Run or Run Preview button when finished Make sure to set a target volume Select your syringe manufacturer and size as previously instructed Using The PHD ULTRA Syringe Selection Screen showing Gang button Gang count entry screen Quick Start screen showing gang number equal to 4 syringes NOTE Possible flow rates and target volumes will change depending on the number of Y syringes in the gang Previously entered parameters may need to be reset Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 34 Using The PHD ULTRA 35 HARVARD APPARA TUS change Current settings 16 57 69 11 32 29 AM Infuse Withdraw Gang 4 syringes Custom 10 30 mm 100 00 ml Ready to infuse Press the green Target 5 ml Run button to start the infusion Infuse 3219 35 ul min or press the back button to ons with
80. een display This section discusses important conventions used in this guide Capitalization All menus commands and dialog box option names appear with initial capital letters whether or not they are completely capitalized in the user interface This includes navigation buttons and menu selection buttons selection lists on the touchscreen display This will help you to distinguish these items from narrative or procedural text Terminology Throughout the PHD ULTRA documentation the following terms are used to refer to program elements and the actions that you perform to carry out tasks e Method A Method is the collection of steps and parameters that constitute the operating instructions to the pump while being used to infuse or withdraw liquids e Choose The terms choose and select indicate actions that you perform using the touchscreen interface Choose represents carrying out an action associated with a parameter or navigation button Choose will be used when the choice you make is automatically executed For example Choose the Method Select button automatically brings you to the Method Selection screen on the touchscreen When you are likely to use the touchscreen to enter alphanumeric data e g a Method name or a parameter numerical value through a simulated keyboard or number pad the specific key is mentioned or we indicate you should enter your desired values e Select The term select refers to highlighting an
81. emoved from a stand alone unit will also cause power to be removed from all RS 485 powered units deriving power from it NOTE Power may be removed from any group of pumps except the first group without affecting communications down stream ds Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 115 Remote Gradient Systems Remote systems consist of a control box and remote unit attached via an RS 485 cable Gradient systems consist of up to two pumps satellites or stand alones attached to a master pump via RS 485 cables Gradient System Connecting a Remote Satellite Pump When a remote pump is connected using an RS 485 cable to a control box or stand alone ULTRA a pump control button representing the remote unit will be displayed on the Method Main or Quick Start Main screen In this button is a number that represents the remote s address Gradient systems with two satellite units attached through an RS 485 cable will have two buttons displayed one for each satellite HARVARD APPARA TUS 03 30 10 02 12 58 PM Select the desired syringe and Method Main screen Sherwood 140 ml 37 95 mm sbowing an active remote witb an address of 01 Stepped 7 Constant Rate HARVARD APPARATUS 1 step s Force Level 100 03 30 10 02 22 52 PM Method Main screen BD plastic 60 ml 26 59 mm showing a Gradient system with 2 active satellites addressed e afo
82. ence position and not the function that currently occupies that position For example if you create a Go To jump to Step 4 in a Method currently a Bolus Pump Operation Profile and subsequently move a Constant Rate profile into the Step 4 position the Go To jump will then connect to the Constant Rate operation and not to the Bolus operation TO INSERT A GO TO JUMP From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the Go To jump to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen 2 oe 2e due x Choose Go To to insert the Go To Step and view an updated list of all current Steps in the Method HARVARD APPARA TUS 04 28 09 1 40 25 PM tep 2 Select the desired step to ga to 100 2 nl rnin and then press the Enter button green check mark to accept or Advanced Options Go To Step 50 ml min press the Cancel button red X screen Choosing the Go To ta cancel your settings and return Command brings you to a listing of the available steps in the Method to the previous screen 6 Double tap the Step that you want to jump to with the Go To Command The system automatically returns to the Method Steps Summary screen which now shows the Go To Step inserted in the Method Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series
83. eparately Universal Power Input RS 485 Connectors RS 232 Serial Input USB Serial Input For pump to pump communication For communication from PC Rear view of the PHD ULTRA pump showing important connections Half nut Release Knobs Pusher Block Guide Rods Syringe Barrel Clamp Vertical Foot lt amp Syringe Holder Support Pp Bracket Clamp Knobs Mechanical Run LED Stops ES User Interface A Touch Screen Lead screw HARVARD Front view of the PHD ULTRA pump showing important components and controls Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 13 POWER CONNECTIONS AND PUMP STARTUP CAUTION Do not connect to firewire devices Damage may occur to pump or device The operating voltage range for the PHD ULTRA is 100 240 VAC 50 60 Hz Use only the specified line cord for this product and make sure line cord is certified for country of use Plug the power cord into the Universal Power Input connector on the rear of the PHD ULTRA Turn on main power switch located in the upper left corner of the rear panel 3 The PHD ULTRA touchscreen display will illuminate and display the startup screen while performing initial self diagnostics When complete the unit will display either the Quick Start screen or the Method Main screen REMOTE PUMP PHYSICAL CONNECTIONS RS 485 Remote System To connect an RS 48
84. er has been set The pump will start automatically on the day time selected Run Preview Access the Method Preview screen that shows a summary of steps and operating parameters Run Resume Used to Run or Resume a Method Re Run Clears counters timers and runs the selected method from the beginning Stop Used to stop running a Method Clear Clears the counters timers Minimum Value Allowed Used to select minimum value allowed Maximum Value Allowed Used to select maximum value allowed pe e lt gt o Ed E R Setting Up The PHD ULTRA 17 Page Down Used to page down in a display list Page Up Used to page up in a display list Scroll Up Used to scroll up in a display list Scroll Down Used to scroll down in a display list Insert Step Used to insert a new Step in a Method while editing creating the Method The new Step is inserted after the Step that is currently selected Delete Step Used to delete the selected otep in a Method while editing creating the Method To confirm deletion you must select the Delete Step button a second time Move Step Up Moves the currently selected Step in a Method up one position in the Method Order e g Step 3 becomes Step 2 Move Step Down Moves the currently selected Step in a Method down one position in the Method Order e g Step 2 becomes Step 3 Copy Step Creates a duplicate copy of the currently selected Step in a Met
85. erior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the clear display window For more efficient cleaning use a soft cloth dampened not soaked with water an aqueous solution of 75 isopropyl alcohol or a mild detergent Chemical Compatibility Chart Overlay Overlay Chemical Concentration Cover Adhesive Chassis gt gt gt Acetonitrile Methyl alcohol Hydrochloric Acid Bleach Windex Isopropyl Alcohol Dish Soap Motor Oil Simple Green Gasoline Ethylene Glycol Antifreeze Mineral Oil Ammonia Magnalube Superlube Acetone Soda Coca Cola Sulfuric Acid Dimethyl Sulfoxide Sodium Hydroxide Nitric Acid Chloroform UU FO UD m50l5m5l5ID55 5D D r UU OD USF USF FKFPrFPrFVSF FFU SS gt UO Ba Ba Ba Ba Ba a Ba ea Ue The chemical resistance data provided is for reference only The data is based on the manufacturer s recommendations and is not a A Satisfatory D Severe Effects guarantee of chemical compatibility for your test conditions Wipe B Fair Minor Effects not tested any spills immediately C Poor Moderate Effects Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 118 Appendices 119 Fuse Replacement Key Information 1 Make sure the power cord is disconnected from the main supply before servicing the fuse 2 Use only Type 3AG 1 4 x 11 4 amp 250 volt Type T time delay fus
86. es Syringe Size 2 5 ml 8 ml 20 ml to 200 ml With SWAGLOK Diameter 1 16 inch With Luer Lock Diameter 1 4 inch Diameter 1 8 inch 70 2269 10 2267 70 2251 70 2255 70 2259 70 2263 Replacement Tips Furnished with Sealing O Ring 70 2246 70 2246 10 2247 Publication 5419 002 Rev E N A 70 2268 70 2252 70 2256 70 2260 70 2264 N A 70 2245 70 2248 Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual N A N A 10 2253 102257 70 2261 70 2265 N A N A 70 2249 N A N A 70 2254 70 2258 70 2262 70 2266 N A N A 70 2250 APPENDIX C MIN MAX FLOW RATE Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on syringe manufacturer Syringe Size 1 pl 2 pl 5l 10 pl 25 pl 50 ul 100 ul 250 pl 500 pl 1000 ul 1 ml 2 5 ml 3 ml 5 ml 8 ml 10 ml 20 ml 30 ml 50 ml 100 ml 140 ml Syringe ID 0 103 mm 0 146 mm 0 206 mm 0 343 mm 0 485 mm 0 729 mm 1 030 mm 1 457 mm 2 304 mm 3 256 mm 4 608 mm 4 699 mm 4 851 mm 8 585 mm 11 989 mm 9 525 mm 14 427 mm 19 050 mm 21 590 mm 26 594 mm 35 700 mm 37 948 mm Publication 5419 002 Rev E Minimum Rate 1 500 3 000 6 000 16 620 33 300 75 240 150 300 300 720 752 100 1 502 3 008 3 128 3 4 13 29 490 51 418 66 043 100 205 180 575 204 033 Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Units pl m
87. es Harvard Apparatus Part No 5146 037 or equivalent Turn off power and remove power cord from power module Use a straight blade screw driver to pry open the access door Remove the fuse holder and then remove the fuses from this holder as shown in figure below Replace fuses and then replace the fuse holder Battery Replacement 1 Remove screw and swing cover to the side 2 Slide battery out from under clip 3 Install Lithium Coin Battery 3V 16mm CR1620 Harvard Part No 5155 288 or equivalent by sliding under clip with positive side facing out CAUTION Use only same type amp rated battery Observe polarity when installing yz NOTE Does not apply to Remote Satellite units Tilt Calibration Pumps with software versions prior to 1 2 1 will require a calibration of the tilt sensor after a software upgrade Steps to calibrate tilt sensor 1 Place the pump on the horizontal orientation 2 Type tilt from hyperterminal 3 Upon completion the pump will display a value no action is required with this value it is for internal use only Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 120 Troubleshooting Infusion Accuracy To ensure infusion accuracy always use new syringe s and measure syringe bore diameter and enter actual dimensions in millimeters mm using the Custom Syringe entry option Additionally make sure that the g
88. et Other value enter the desired force setting as a percentage value 1 100 then choose Accept to confirm your setting 5 When you have selected or entered the desired force value choose Accept to return to the Settings menu Suggested Force Level Settings for Common Syringes Syringe Type Material Capacity Force Setting Common Manufacturer s Plastic Syringes 20ml 50 BD plastic Sherwood Airtite Terumo 20ml 10096 Stainless Steel All 100 Harvard Apparatus Glass Glass 20ml 20 Cadence Popper 20ml 3096 Glass Plastic lt 1000ul 30 Hamilton SGE 5ml 5096 5ml 10096 Suggested Force Level Settings for Common Syringes Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 22 Setting the Time and Date The PHD ULTRA displays the date and time on each screen in the user interface Built in battery backups maintain the date and time even if the pump loses power or is unplugged for a period of time If it is necessary to set the date and time values follow the instructions below TO SET THE DATE AND TIME DISPLAY 1 PA 3 From the Quick Start or Method Main screen choose the Settings button On the Settings screen choose the Date Time Display box to enter the Set Date and Time screen To change the Date select the Date button and enter the Date values using the keypad in the format defined by the da
89. evices This section outlines the use of these commands hs NOTE Do not attempt to Export Import Methods while the pump is running Do not change screens while Importing Exporting a method BZ NOTE For additional details on the physical interfaces used to connect the PHD ULTRA to external devices refer to the External I O Connections section in the Appendices BZ NOTE Before downloading or transferring a method to the pump verify the number of steps available on the pump information screen HARVARD APPARATUS 04 28 09 1 44 48 PM The method has been exported Export Method confirmation screen showing successful PRESS TO CLEAR MESSAGE export TRANSFER A METHOD BETWEEN PUMPS Follow these instructions to transfer a Method between two PHD ULTRA pumps 1 Connect two pumps together via a RS 485 firewire type cable and set the address on the first pump to 00 using the Set Pump Address command in the Settings menu Set the address on the second pump to something other than 00 using the Set Pump Address command in the Settings menu On the first pump from the Method Main screen choose Method Select then choose the Method you would like to export Choose the File Options button then choose the Pump button and enter the address of the pump you would like to receive the Method Choose Export Method to Pump The pump will display a confirmation message in the Message Area on the screen when
90. f If Withdrawing at xl xxx lt cr gt Ift prompt DIAMETER Sets or displays the syringe diameter in mm Quick Start mode only Command format diameter syringe diameter Query response If 7 mm lt cr gt It prompt GANG Sets or displays the syringe count Quick Start mode only Command format gang syringe count Query response If syringes lt cr gt If prompt Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA RATE COMMANDS CONTINUED IRAMP Sets or displays the target infusion rate while ramping Quick Start mode only Command format iramp start rate start units end rate end units ramp time in seconds Query response If Ramp not set up lt cr gt If prompt Of If Starting rate xl xxx lt cr gt lt If gt Ending rate xl xxx lt cr gt It 4 Ramp time seconds lt cr gt If prompt NOTE When using the iramp command rate time remaining on display will not reflect A actual pump rate time remaining during ramping IRATE Sets or displays the infusion rate Quick Start mode only Command format irate rate rate units max min Query response lt lf gt xI xxx lt cr gt lt lf gt lt prompt gt WRAMP Sets or displays the target withdraw rate while ramping Quick S
91. f the defined syringe the system will prompt you that parameters are out of range The system also prompts users if required values are not entered NOTE The pump will check the validity of parameters entered for each Step in the Method but will not review the Method in its entirety to verify that it is logical and can be fully executed Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 44 STEP BY STEP OPERATION EXAMPLE USING A PRELOADED OR USER DEFINED METHOD The following steps describe operation using a Preloaded or User Defined Method In the following description we are assuming the use of a Method that is already saved on the PHD ULTRA Note that if you make any changes to the Method s operating parameters and choose Accept on the page where the changes are made the changes automatically become part of the stored Method for future use 1 From the Method Main screen the Method you wish to use should be displayed in the Method Select button If the Method is not displayed select the correct Method using the procedure outlined in Running The Pump 2 Choose the Syringe Select button to enter the Syringe Selection screen 3 Follow the instructions in the Syringe Selection section of this Guide to complete the selection of a syringe from the built in Syringe Lookup Table or to create a new syringe When finished choose the Accept button to save your changes
92. f the pumping cycle e The Target Time that determines the total length of time over which the stepped profile will be calculated e The Number of Steps that will be created in the profile During a Stepped Rate profile the PHD ULTRA calculates a series of equal rate increases that will vary the flow from the starting value to the ending value You enter the Number of Steps you want to use The Target Time defines the total length of pumping time with the Target Time value divided equally into each stepped rate A Stepped Rate profile can be applied to an increasing or decreasing rate application When creating or editing a Stepped Rate profile you can set the rate in ml ul nl or pl units of volume using sec or h m s hours minutes seconds as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 55 TO SET UP EDIT A STEPPED RATE PROFILE 1 From the Step Definition screen for a Stepped Rate profile choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation 2 Choose the Set Start amp End Rate button to enter the Set Start and End Rates screen HARVARD APPARA TUS Enter your settings to the left and then press
93. fea 62 sant OF Ramp OP ily is being controlled by the master Total Yolume Dispensed O ml E 4 Dm Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 116 The pump control button s background color indicates the status of the remote unit e Blue Background Connected Motor Stopped e Red Background Comm Error e Yellow Background Motor Stall e Green Background Motor Running The user can select which pump to control by pressing the button with its corresponding address The currently selected pump s button will contain an interior white box When a satellite pump is being controlled the Fast Forward Fast Reverse buttons on the master will move the satellite s pusher block HARVARD APPARATUS 03 30 10 02 13 48 PM showing a comm error with the remote pump addressed O1 Sherwood 140 ml 37 95 mm Stepped Constant Rate Setting Addresses on Remote Satellite Units To change the address of a remote or satellite unit you need to communicate directly to the pump through a USB cable 1 Connect the Remote Satellite unit to a PC via a USB cable 2 Launch HyperTerminal 3 Set address using the appropriate Pump Chain Commands p NOTE Remote units are set at a default address of 1 Setting the Address on the Control Box Master Unit The address of the control box or master unit must always be set to O zero 1 On the pump s touch
94. force changed at Master e will not change Force Setting at Satellite Must set thru USB if different Force is desired at the Satellite s NOTE Make all cable connections then power up the satellite pump before powering up the master pump TO SET UP EDIT A GRADIENT PROFILE 1 From the Step Definition screen for Gradient choose Set Combined Flow Rate to enter the Set Rate screen HARVARD APPARA TUS 10 2709 11 54 15 AM Set Addresses and Flow percentage information Gradient Step Definition Screen DO seconds Ramping Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 63 2 Enter the desired combined flow rate value for all pumps using the keypad or select the Minimum Rate or Maximum Rate button HARVARD APPARA TUS N 10 27 09 11 54 35 AM Enter your rate and units and then press the Enter button green check mark to accept or O ys press the Cancel button red X Combined Flow Rate Entry ta cancel your settings and return 220 975 to the previous screen screen d ml min 212 792 ml min 3 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 5 When all parameters are correctly set on the Set Rate screen choose Accept to confi
95. fuse D CD E Syringe Pump to Valve Tubing Connection Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 130 APPENDIX K ORDERING INFORMATION Standard Version Description Stand Alone Remote Satellite PC Box System System PHD ULTRA Infuse Only 70 3005 70 3305 N A N A PHD ULTRA Infuse Withdraw 70 3006 70 3306 70 3406 70 3506 PHD ULTRA Infuse Withdraw Programmable 70 3007 70 3307 N A N A Push Pull Versions Description Stand Alone Remote Satellite PC Box System System PHD ULTRA w Push Pull Mechanism 70 3008 70 3308 70 3408 70 3508 PHD ULTRA Programmable w Push Pull Mechanism 70 3009 70 3309 N A N A Rack Options Catalog Description 70 3020 6 10 Multi Rack Holds up to 10 syringes from 0 5 pl to 20 ml and up to 6 syringes from 30 ml to 60 ml 70 3021 4x140 Multi Rack Holds four 60 ml or 140 ml plastic syringes only 70 3022 Microliter Multi Rack Independently holds 4 syringes from 0 5 ul to 10 ml 70 3023 Anti Siphon Kit Options Catalog Description 70 3030 PHD ULTRA RS 232 RJ 11 Connectors Optiont 70 3031 PHD ULTRA Internal Pinch Valve Option 70 3036 PHD ULTRA Remote Satellite Internal Pinch Valve Option 70 3032 PHD ULTRA Internal 3 way Isolation Valve Option 70 3037 PHD ULTRA Remote Satellite Internal 3 Way Isolation Valve Option 70 3033 PHD ULTRA Analog Control Input Opti
96. g the T knobs 9 Then push the Pusher Block s Retaining Bracket 2 tight against the plunger flange and tighten using the Bracket Clamping Knob 4 Y NOTE Mechanical stops are set using the same steps as a standard 2 syringe holder Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 106 Microliter Multi Rack Configuration Syringe Loading Push Pull Mechanism Thumb Nut Syringe Holder Adjustable Syringe Holder Syringe Clamp Bracket Clamping Knobs Syringe Holder Bracket Clamping Knob Pusher Block Retaining Bracket Syringe Holder Retaining Bracket Pusher Block Thumb Nut HARVARD Syringe Clamp PHD ULTRA showing Pusb Pull Mecbanism installed To load syringes on the push pull pump 1 Loosen Thumb Nut 1 to release the Adjustable Syringe Holder 2 2 Loosen the Retaining Bracket 7 on the Pusher Block using the Bracket Clamping Knob 5 3 Loosen the Retaining Bracket 6 on the Adjustable Syringe Holder 2 note that there are bracket clamping knobs 4 on both the front and rear of this assembly that need to be loosened 4 Extend Plunger on syringe s to maximum capacity Loosen Thumb Nut 8 and lift Syringe Clamp 3 Place the syringe on the Adjustable Syringe Holder 2 extend as necessary then lock into place by tightening the Thumb Nut 1 Lock the syringe flanges into place by tightening the Retaining Brackets 6 7
97. g use Over time you may want to manage previously created Methods to keep your Method List easy to navigate In addition the PHD ULTRA allows you to export Method information to an externally connected pump or to a computer that will be used to control operation This section of the Guide describes the file management facilities available on the PHD ULTRA Y NOTE The method management procedures outlined in this section are not available V tor Quick Start or Analog Mode Methods Saving A Copy Of A Method Follow these instructions to save a copy of a Method under a new name 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want to save a copy of then choose the File Options button to enter the File Options screen 3 Choose Save As Copy to access the Method Name Entry screen The current Method Name will be displayed in the text entry box 4 Onthe Method Name screen type the new name for your Method up to 15 characters using the onscreen keyboard Choose the Symbols Numbers button 123 to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters mode choose the Letters button ABC 5 When finished entering the new Method Name choose Accept to save your changes and return to the File Options screen 6 Choose Ba
98. ge Start Refill p Direction Output TTL Logic Output High Infuse Low Refill 8 Valve Output TTL Logic Output High Valve Actuated Low Valve Off NOTE Valve follows direction change when set to auto 9 13 Signal Return Ground 14 Run Indicator Voltage 5V through a 470 ohm resistor connect to LED Anode 15 Run Indicator Output TTL Logic Output active low low run connect to LED Cathode Electrical Specifications Inputs Vin gt 2V L lt 20pA Va lt 0 4V I lt 0 5mA Outputs V 3 8V I 6mA Vo s 0 4V I lt 6mA Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 109 Analog Input Option HARVARD APPARA TUS lia AS Welcome to the Analog Control Screen Press Enter button green check mark to accept or press the Cancel button red X to return to the previous screen Flow j Analog Control Screen Direction E a Speed 0 Signal Description GND Ground IN SIG RTN Signal RTN N C IN ANALOG IN 0 10 V Analog In ANALOG IN SIG 0 to 10V OVERVOLTAGE RATING Absolute Max Min 15V Max 2 5V Min 4 Pin miniDIN Female Analog control of the motor speed can be achieved in many ways The following circuit is recommended A DC voltage 10 VDC max will be needed to supply the excitation voltage to the circuit DC Voltage Source Mating 10 VDC max Connector Multi turn Potentiometer Refer to the accessories s
99. ge Type Material Capacity Force Setting Common Manufacturer s Plastic Syringes 20ml 50 BD plastic Sherwood Airtite Terumo 20ml 10096 Stainless Steel All 10096 Harvard Apparatus Glass Glass 20ml 20 Cadence Popper 20ml 30 Glass Plastic lt 1000ul 30 Hamilton SGE lt 5ml 50 5ml 10096 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 95 APPENDIX B STAINLESS STEEL SYRINGES Cat No Product Replacement Parts 20 to 200 ml 9013 089 9013 090 9013 091 9013 092 9013 109 72 2472 72 2473 72 2474 72 2475 72 2616 72 2617 Chemraz O Ring 20 ml Chemraz O Ring 50 ml Chemraz O Ring 100 ml Chemraz O Ring 200 ml Chemraz Tip Seal O Ring sizes 20 200 ml Replacement Viton O Ring 20 ml pkg of 10 Replacement Viton O Ring 50 ml pkg of 10 Replacement Viton O Ring 100 ml pkg of 10 Replacement Viton O Ring 200 ml pkg of 10 Replacement Viton Tip Seal O Ring sizes 20 200 ml pkg of 20 Stainless Steel Plunger Button to Adapt Syringe for Use with PHD 22 2000 HVP with Triple 5 Clamp Required for 50 ml and 100 ml syringes only Harvard High Pressure Stainless Steel Syringes Harvard offers a complete line 2 5 to 200 ml of Stainless Steel Syringes intended for high pressure applications with good resistance to most aggressive liquids Wetted parts are 316 stainless steel or Viton Syringes ar
100. ge of motion pull the handle out and twist counter clockwise then allow the handle to click back into place 2 Once both handles have been loosened tilt the DIN Rail to your desired angle and tighten into place using the Tilt Adjust Handles NOTE Ensure that the DIN Rail is tightened securely using the Tilt Adjust Handles to prevent any movement when the pump is attached Mounting a Pump 1 The rear side of a Satellite Remote pump has a Groove with a Locking Spring for DIN Rail mounting Position the pump s top groove and locking spring over the top edge of the DIN Rail 2 Push the top of the pump down against the spring while pushing the bottom of the pump back until the bottom groove locks under the DIN Rail Dismounting a Pump 1 Holding both sides of the pump push down on the top of the pump to depress the Locking Spring 2 Tilt the bottom of the pump out to release the bottom Groove from the DIN Rail 3 Lift the pump off of the DIN Rail Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 128 Appendix I Valve Option ULTRA showing the internal 3 way isolation valve option catalog no 70 3032 Port size is 4 28 Three 1 16 barb fittings are included ULTRA remote unit showing the internal pinch valve option catalog no 70 3031 Loading tubing into an internal pinch valve While valve is not actuated load tubing into the inside slot Then using the
101. ger out command Trigger 1 Specifies output via the Trigger 1 Pin 4 port When selected you must also specify whether the signal to be sent is High Signal or Low Signal High Signal Sets the output signal to logic high Low Signal Sets the output signal to logic low e Trigger 2 Specifies output via the Trigger 2 Pin 5 port When selected you must also specify whether the signal to be sent is High Signal or Low Signal High Signal Sets the output signal to logic high Low Signal Sets the output signal to logic low e Synchronize Select this to send a signal via Pin 6 to a connected pump to trigger activity When selected you must define whether the desired action is to start an Infuse activity or a Withdraw Refill activity Start Infuse Defines the rising edge signal to trigger an infusion action Start Withdraw Defines the falling edge signal to trigger a withdraw refill action e Valve Select automatic or manual valve control automatic is controlled by pumping pin 8 Valve On Activates the valve Valve Off De Activates the valve Valve Auto Returns the pump to Automatic valve control Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 72 TO INSERT AN I O TRIGGER OUT COMMAND 1 2 3 4 5 6 From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select onc
102. gh creation editing of a custom Pump Control Method Pump Control Methods consist of the following set of operating parameters The Method Name e Syringe Information e The set of Pump Operation Profile steps that describe how the pump will control the flow of material for infusion or withdrawal operations e Any additional commands to control the execution of the steps e Any input output I O triggers responses used to control external devices or respond to input signals from external devices Both the Preloaded and User Defined Methods offer the user the flexibility to apply pumping profiles other than continuous rate infusion withdrawal The PHD ULTRA offers a menu of advanced pumping profiles including e Constant Rate a single continuous rate of flow e Ramped Rate a linearly changing flow rate between a starting and ending flow rate e Stepped Rate an evenly stepped changing flow rate between a starting and ending flow rate e Pulsed Flow an oscillating flow rate varying between a starting and a target rate e Bolus Delivery an infusion operation to occur for a specified volume and time The Infusion Rate is automatically calculated e Concentration Infusion a measured flow rate based on delivery of a specific dosage to a recipient based on the recipient s weight e Gradient Combined flow streams from mutiple pumps into a common output stream e Autofill Cycling continuous infuse withdraw or withdraw infuse operatio
103. hod and inserts it as the last Step in the Method being created edited Touch Screen Unlocked Indicates that the Touch Screen is currently unlocked Pressing the button twice locks the Touch ocreen Touch Screen Locked Indicates that the Touch Screen is currently locked Press the button and enter password to unlock the Touch Screen File Options Access the File Options menu that is used to manage the list of Methods and to export Method information to an externally linked pump or to a connected computer Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA SYSTEM SETTING ADJUSTMENTS The PHD ULTRA Settings menu is used to control basic system operations This section describes the options available in this section of the application software HARVARD APPARA TUS ade 10 27 09 12 00 05 PM Change Logo Select the desired setting and Audible Pump then press the Enter button Te green check mark to accept or press the Cancel button red X Baud Rate to return to the previous screen Tbe Settings screen 115200 Address O Set Password a Running g Y Setting the Audible Alarms The PHD ULTRA pump incorporates an Audible Alarm feature that can be used to warn the operator about various operating conditions errors and power outages The following alarms are user configurable in the Settings section of the application e End of Run An alarm will sound when a Method r
104. i optional STAND ALONE Mate 1 MASTER SATELLITE SATELLITE ADOR 00 ADOR 01 ADDR 02 AC Power RS 4a5 OUT RS 485 R3 485 IH OUT IH MUST BE ADDR ADDRESSES CAM BE 01 99 Master Satellite operation using satellite remote units PE optional CONTROL R5 485 REMOTE RE 485 REMOTE R5 465 REMOTE Mote 1 Box SATELLITE SATELLITE SATELLITE ADDR 00 ADDR 02 ADDR 03 euros RS 486 E R5 425 R5 405 OUT E IH OUT IH MLIST BE ADDR D ADDRESSES CAN BE 01 99 Gradients using remote system NOTE PC Control via HyperTerminal application Limited command set p irun wrun stop stp When using stand alone units as satellites do not connect the line cord and leave the power switch in the OFF 0 position Connect units using an RS 485 cable OUT to IN Power up Master Unit Master and Satellites will all power up and Satellites should indicate Satellite XX on their displays where XX represents pump address When using remote units as satellites power switches all operate as indicated turn ON each pump on the RS 485 network NOTE If it is desired to power a Stand Alone unit as a Satellite by using the line cord p and internal power supply connect line cord and set power switch to ON 1 position NOTE If a satellite powers up but displays the Main GUI screen instead of Satellite XX verify that the address of the Satellite is not set to 00 hs Y NOTE Each satellite must have a uniq
105. ibration is suggested once per year so choosing Reset will modify the Calibration due date to one year from the current date 5 The pump will display the total run time since the last reset of the Lubrication timer To reset the time choose the Lubrication Reset button 6 When you are finished choose Accept to return to the Settings menu Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 26 Power Up Running The Power Up Running Setting is designed to allow the pump to restart pump operation automatically after a power outage when operating in a continuous mode The Power Up Running selection is only valid under the following conditions e When operating in a Quick Start Infuse Only or Withdraw Only method e When the pump is running at the time of a power loss e When no target Volume Time is set TO SET POWER UP RUNNING 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu select the Power Up Running button The checkbox on the Power Up Running button will be checked to indicate that the function is on To toggle the function off select the button again 3 When the Power Up Running button is set as desired choose Accept to save your changes and return to the Quick Start or Method Main screen NOTE Counter Timer information is reset when power resumes Counter Timer Y information prior to p
106. ile Menu screen The available options include a Constant Rate c Stepped e Bolus g Gradient i Advanced Options b Ramp d Pulse f Concentration h Autofill HARVARD APPARA TUS 10 27 09 11 54 21 AM Select the desired step profile or press the Cancel button red X to return to the previous screen Method Steps Profile Menu screen Advanced Options 8 Choose the Method Step type that you want to add to the Method If you choose a pumping profile e g Constant Rate Ramp etc you will be taken to the Step Definition screen for the selected Method If you choose Advanced Options you will taken to the Select Advanced Options screen 9 Complete the parameter entry applicable to the selected Pump Operation Profile follow the detailed instructions supplied in the Pump Operation Profiles Preloaded and User Defined Methods section later in this Guide If you selected Advanced Options follow the instructions supplied in the Incorporating Advanced Options Into a Method section later in this manual gy NOTE When setting a Target Time for a method step the minimum length for a stand alone ULTRA is 0 2 seconds When using a remote system the minimum length for a method step is 0 5 seconds to allow for communication Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 48 10 When you have completed all parameter entries on the Step Definition screen or
107. in pl min pl min pl min pl min pl min pl min pl min pl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min Maximum Rate 1 589 3 180 6 358 17 630 35 249 79 640 158 984 318 126 795 51 1 588 3 181 3 308 3 52 11 044 21 539 13 58 31 190 54 383 69 852 105 985 190 992 215 803 Appendices Units pl min pl min pl min pl min pl min pl min pl min pl min pl min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min 96 Appendices 91 APPENDIX D SYRINGE HOLDER OPTIONS Mounting a 4x140 Multi Rack Clamp Bar Knob Multi Syringe Clamp 2401 071 2401 076 t 10 32 x 1 2 Screw 4 5101 087 Multi Holder 2401 074 Multi Retainer 2401 070 Lock Washer 2 Bracket Syringe 5174 003 Clamp 2401 050 Flat Washer 2 5110 038 T Knob 2 5100 05 Dual Syringe Holder 2402 007 4x140 Multi Rack Syringe Holder Assembly Remove the four Set Screws from Syringe Holder using the provided hex key Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob Place the Multi Holder assembly on the Dual Syringe Holder and attach using four 10 32 x Screws ps e e ue Attach Clamp Bar by screwing the Multi Syringe Clamp Knob into the threaded bar attached to the Multi Holder Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA
108. inge flanges then tighten the retaining brackets using the Bracket Clamping Knobs 7 8 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the right until the syringe plunger is located as far into the syringe as it will travel Using the Allen Wrench located in its holder in the rear of the Pulley Cover 11 loosen the Infusion Mechanical Stop 9 and slide it to the left until it is in contact with the Pusher Block 2 Use the Allen Wrench to tighten it in this position Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the plunger is located just to the left of the maximum volume allowable Use the Allen Wrench to loosen the refill Mechanical Stop 10 and slide it to the right until it is in contact with the Pusher Block 2 Tighten the Withdraw Mechanical Stop 10 and then return the Allen Wrench to its holder 11 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 28 F Mechanical Stop Mechanical Stop Syringe fully mounted and locked in place Note mechanical stops set in position to prevent excess travel of pusher block CAUTION Be sure the mechanical stops are postioned properly to prevent the plunger from bottoming out on glass syringes Changing the bracket for small lt 30ml vs large gt 30ml syringes 1 Remove the Syringe Barrel Clamp Assembly by completely unscrewing the Syringe Barrel Clamp Kno
109. ion provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use The operating voltage range for the PHD ULTRA is 100 240 vac 50 60 Hz See Note 1 Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded See Note 1 Make Proper Connections Make sure all connections are made properly and securely Any signal wire connections to the unit must be no longer than 3 meters except RS485 pump to pump communication cable Observe All Terminal Ratings Review the operating manual to learn the ratings on all connections Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Avoid Pinch Hazard A pinch hazard may exist between the pusher block and end blocks Avoid placing fingers between these points while the pump is running Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qualified service personnel to perform inspection Orient the Equipment Properly Do not orient the equipment so that it i
110. lder assembly on the Dual Syringe Holder and attach using four 410 32 x Screws Attach Clamp Bar by screwing the Multi Syringe Clamp Knob into the threaded bar attached to the Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 100 Watcher Flat 2 5 10 038 Retaining Ring Shaft Brocket Clamp 5007 038 2401 023 Bracket AntiSyphon CC Thumbsorew 3 2401 025 Y 100 012 Ext Tooth Lockwasher 2 2 TS usher Bar 5174 003 2401 066 Knoo Bracket Clamp 2401 031 Spring 3 5009 164 Bracket Ant Syphon 2401 065 Washer Flat 3 5 43 003 bracket Hold Down 2401 048 Thumbscrew 2 51 lat Head Screw 2 5137 015 6 10 Multi Rack Pusber Block Assembly 1 Remove Dual Syringe Anti Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft 2 Attach the Pusher Bar assembly to the Pusher Block using two flat head screws Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 101 Syringe Loading 4 x 140 amp 6 10 Multi Racks Helease Tabs Pusher Block Syringe Holder Retaining Bracket Syringe Holder Anti Syphon Bar Pusher Bar Clamp Bar T Knobs Mechanical Stop Infuse Mechanical Stop Withdraw Plunger Holder Pulley Cover Allen Wrench Holder Front view of PHD ULTRA with 6 10 Multi Rack showing controls for multi rack syringe loading
111. link to the desired method upon a Withdraw activity Infuse Will link to the desired method upon an Infuse activity TO INSERT AN I O EVENT LINK IN COMMAND From the Method Main screen choose Step Definition to enter the Method Summary screen Highlight select once the Method Step that you want the I O Event Link in command to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Select Advanced Options screen Choose the I O Event Link in button to enter the Event Link Step Definition Screen po uM eme X cs ed Select the desired Event type that you want to have the event associated with from the options available Input Low Input High User Press Motor Stall Withdraw Infuse 7 After selecting the desired option in the Event Link screen choose Accept to access the Method Steps Summary selection screen Choose the Method name to jump to To use this command you must have defined a linkable Method 8 Choose the Accept button to save your changes and return to the Method Steps Summary screen NOTE Since the Event Link will cause a jump link to a different method be cautious Le not to use embedded links in your method programming Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA T5 MANAGING EZ PRO METHODS All Preloaded and User Defined Methods are stored in the Method List for ongoin
112. mp the PHD ULTRA requires that each method include accurate setting of the syringe diameter and volume All Methods Quick Start Preloaded User Defined require a syringe definition either by selecting from the installed syringe specifications list or by defining a new Custom syringe in the application The built in Syringe Lookup Table provides a convenient lookup function that allows users to select from a wide variety of syringes from major manufacturers including Air Tite Becton Dickinson Hamilton Harvard Apparatus Cadence Science SGE Scientific Glass Engineering Sherwood Monoject and Terumo This Guide also includes a reference table of major syringe diameter and volume parameters in Appendix A Syringe Volume Diameter Reference Table The following parameters must be set for every syringe selection e Syringe Diameter mm e Syringe Volume ml or pl high viscosity liquids you should adjust the Force value in the Settings j WARNING When using low volume small diameter syringes or pumping menu to avoid accidental damage to the syringe NOTE For Master Satellite configurations changing diameter at the Master will Y change the diameter at all satellites TO SELECT A SYRINGE USING THE SYRINGE LOOKUP FUNCTION 1 From the Quick Start or Method Main screen choose the Syringe Select button HARVARD APPARA TUS Select an existing syringe HS Norm Ject manufacturer or select custom syringe and then press Enter Gla
113. mp Control Method The Pump Control Methods will be commonly referred to as Methods A Method defines e Details about the syringe being used for the application e How rapidly you will be dispensing or withdrawing the target fluids e A target volume or target time for the dispensing or withdrawing operation e The pumping pattern you want to employ e g pump at a continuous rate in a ramped fashion using variable rate steps etc e Outbound triggers to external devices e Responses to signals from external devices The PHD ULTRA is shipped with a set of pre installed Methods that are designed to help you to quickly use the pump in common applications These pre installed Methods include Quick Start Methods which are simple infuse withdraw or combination infuse and withdraw Methods that operate in a single step and at a constant rate of flow e Preloaded Methods which use more complex Method programming capabilities to describe commonly encountered applications NOTE What Methods are available on your pump depends on the specific model you AS are using An Infusion Only model supports only the Quick Start Infuse Method The Infusion Withdrawal model supports all Quick Start Methods and one custom Method The Infusion Withdrawal Programmable model supports all Methods described in this manual In addition users of the Infusion Withdrawal Programmable model PHD ULTRA can quickly create custom Methods to support more complex di
114. n In addition you have access to a variety of Advanced Options that allow the PHD ULTRA to repeat loop back or jump forward in a Method as well as send signals to external devices or react to signals received from external devices This package of pumping profiles and commands provides users with an extremely broad array of applications that can be easily configured without the use of complex external programming The Preloaded Methods provide a starting set of Methods that define some commonly used application protocols These Methods can be edited and saved under a new name or simply edited to fit your specific requirements Creating a new User Defined Method permits you to leverage all the options available on the PHD ULTRA Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Preloaded User Defined Method Workflow Using The PHD ULTRA 43 The following flow diagram describes the use of an existing Preloaded or user Defined Method Select Method Select Syringe Model OR Create New Syringe Type Review Method Steps Pump Operation Profiles Enter Pump Operation Profile Parameters Validate Commands and l O Operations Preloaded User Defined Metbod workflow As with Quick Start Methods the EZ PRO software provides the user with a variety of prompts and warnings to help guide the setup of methods For example if a pump Method incorporates steps that exceed the capacity o
115. n or press the back button to return to the previous screen a HARVARD APPARA TUS 08 18 09 1 32 03 PM Method paused Press the green Run button to resume pumping press the Clear button orange arrow to reset or press the Back button to return to the previous screen Using The PHD ULTRA 36 To Preview a Method press the Method Preview button to enter the Run Quick Start Methods or Method Run Preloaded and User Defined Methods screen Quick Start Run screen for a Infuse Withdraw Method Method Run screen for Preloaded and User Defined Methods The Run Method Run screens contain a summary of the operating parameters as described below Quick Start Run Screen e Method type e Syringe information e Target Volume e Summary of Infuse and Withdraw rates as applicable Initial flow direction Total time for the Method e Force Level setting Preloaded User Defined Metbod Run Screen Method name e Total number of steps in the Method e Syringe information e Summary flow parameters for the previous step current step and next step to be performed in the method e Force level settings 8 Tochange any of the Method parameters choose the Back button on either the Run or Method Run screen or choose the Change Current Settings button on the Quick Start Run screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual RUN THE METHOD Using The PHD
116. n choose Accept to confirm your selections Repeat Steps 3 to 5 for the End Rate by selecting the End Rate button and continuing data entry When all parameters are correctly set on the Set Rate screen choose Accept to confirm your settings and return to Step Definition screen Choose the Set Target Time button to enter the Set Time screen Enter the desired Time value using the keypad 10 Ifthe Units ofTime are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 11 When all parameters are correctly set on the Set Target Time screen choose Accept to confirm your settings and return to the Step Definition screen Y NOTE Ramps lt 2 seconds from min to max rates may cause motor stalls Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 53 Using The PHD ULTRA 54 STEPPED PROFILE The Stepped Rate profile is used to create an increasing or decreasing flow rate that varies in a series of equal steps between a starting and ending flow rate Stepped Rate Pump Operation Profile Time Volume When creating or editing a Stepped Rate profile you will define four primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw e The Flow Rate at the beginning and end o
117. n you have selected the desired baud rate choose Accept to save your changes and return to the Settings menu Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Pump Information Setting Up The PHD ULTRA 25 The PHD ULTRA monitors usage and provides convenient reminders for periodic maintenance or calibration You can adjust the settings for the reminders to accommodate your specific operating conditions Harvard Apparatus recommends that system calibration checks adjustments be performed at least once per year on the pump and that lubrication should be performed once every 100 hours of operation Model PHD Ultra Type Programmable Software Version Program Steps Available 804 807 Calibration Due Date 01 01 71 Maintenance Due 98 hours Pump Address Remote Address HARVARD APPARA TUS 01 20 10 01 03 38 PM Reset the Calibration Date or the Maintenance Due as needed Pump Information screen Y NOTE Free RTOS V6 0 1 www freertos com TO RESET THE CALIBRATION AND LUBRICATION DATE AND TIME REMINDERS From the Quick Start or Method Main screen choose the Settings button From the Settings menu choose the Pump Information button to enter the Calibration screen 1 2 3 Note that the next date for suggested calibration is displayed on this screen at all times 4 Choose Calibration Reset to set the next suggested Calibration due date based on the current date Note that cal
118. nse If Target time not set lt cr gt Ift 4 lt prompt gt or If seconds lt cr gt If prompt Of Ift 7 cr Ift prompt Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 91 Using The PHD ULTRA 92 TIME COMMANDS CONTINUED WTIME Displays the withdrawn time Quick Start mode only Command format wtime Query response If seconds lt cr gt It prompt Of If cr It prompt DIGITAL I O COMMANDS INPUT Reads and displays the trigger input port status Command format input Query response It The trigger input is low lt cr gt It prompt Of It The trigger input is high lt cr gt It prompt DIGITAL I O COMMANDS CONTINUED OUTPUT Sets the level on one of the output ports Command format output 1 2 high low SYNC Sets the level on the sync port Command format sync high low Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 95 INTERNAL COMMANDS STATUS Displays the raw status for use with a controlling computer Command format status The output is in three integer fields and one flag field all separated by spaces and terminated by a carriage return linefeed pair
119. o change the level as desired for your Method Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 15 I O EVENT IN The I O Event in command is used to have the pump pause and wait for a signal from an external device or for a manual user input at the touchscreen These external signals are communicated via Pin 2 on the 15 pin I O Connector port HARVARD APPARA TUS Input High to Low Input Low to High Select which type of event the step will wait forand then press the Enter button green check mark to accept or press the Cancel button red X to return Advanced Options menu I O to the previous screen Event in Selection Screen The following options are available when setting an I O Event in command Input High to Low Sets the pump to wait for a logic low input signal falling edge Input Low to High Sets the pump to wait for a logic high input signal rising edge e User Press Sets the pump to wait for manual input from the user at the touchscreen screen touch TO INSERT AN I O EVENT IN COMMAND From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the I O Event in to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Select Advanced Options screen Choose the I O Event in button to
120. ociated with Duration are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon When all parameters on the Set Dose or Duration screen are correct choose the Accept button to return to the Step Definition screen Choose the Set Doses amp Time Between Doses button to enter the Set Doses amp Time Lag screen HARVARD APPARA TUS oo 04 28 09 1 35 46 PM ae Sees Enter the dose and time info and nus then press the Enter button green check mark to accept or E press the Cancel button red X Set Doses and Time Lag to cancel your settings and return screen in Concentration EI E 6 to the previous screen profile Select the Doses button it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired number of doses using the keypad If the Doses is greater than one select the Time Lag button and enter the desired length of time between individual doses If the Units of Time associated with Time Lag are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon When all parameters on the Set Doses amp Time Lag sc
121. od is now saved on the external computer IMPORTING A METHOD FROM AN EXTERNAL COMPUTER Follow these instructions to import a Method from an external computer 1 A de cep cues Connect the pump to the external computer via an RS 232 or USB cable and set the Address value to 00 using the Set Pump Address command in the Settings menu On the computer open the HyperTerminal application and verify that the computer can communicate with the pump In HyperTerminal click on the Properties icons and choose the Settings tab on the pop up box that is displayed Click on the ASCII Setup button In the Line Delay field enter 500 This will set a 500 millisecond delay after each line is transferred during the import process Click OK twice to return to the HyperTerminal Main Menu screen In HyperTerminal choose Transfer Send Text File Select the Method that you want to import to the PHD ULTRA pump and click Open Before disconnecting verify that the Method was transferred by choosing Method Select from the Method Main screen at the destination PHD ULTRA pump then browse the Method menu to confirm that the new Method is now saved at the pump Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 79 EXTERNAL PUMP CONTROL This section of the PHD ULTRA manual describes the control of the pump using an external computer device Setting Up HyperTerminal
122. ode defining the cycle Infuse Withdraw INF WD or Withdraw Infuse WD INE e The Flow Rates to be used during infuse withdraw operations e The infusion volume to be delivered during each pump cycle The total volume to be delivered during all cycles or the total number of Infuse Withdraw or Withdraw Infuse cycles that you want the pump to execute For example if you want to pump 100 ml using a 10 ml syringe the pump will execute 10 Infuse Withdraw or Withdraw Infuse cycles depending upon the chosen flow mode During an Autofill profile the PHD ULTRA operates at a constant pump rate during the infusion and withdrawal stages although the rate can be different for each direction i e infuse vs withdraw When creating or editing an Autofill Cycling profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Rate and Concentration screen HARVARD APPARA TUS 04 28 09 1 38 44 PM Enter your settings to the left and then press the Enter button INF O ml min WD D ml rnin green check mark to accept or press the Cancel button red X to return to the previous screen Step Definition screen for an Cycle Autofill cycling profile 50 ml per t Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA
123. ods which organize all the commands needed to control the infusion withdrawal process The current settings for the pump are displayed in the selection buttons on the Method Main or Quick Start screens HARVARD APPARA TUS 10 27 09 10 03 47 AM Enter your settings to the left and then press the green Run or Quick Start Infuse Withcdraw acri Run Preview button when Quick Start screen sbowing A See ee sae finished Make sure to set a the Infuse Withdraw target volume Method buttons with current parameters highlighted For Preloaded or User Defined Methods with multiple steps the Method Main screen displays the first three steps and the last step in the Method operation when all steps do not fit on the screen In the following sample screen the first three steps Delay Ramp Constant Rate are displayed in one box button and the last step Ramp is displayed beneath them HARVARD APPARATUS METHOD Select the desired syringe define steps and then press the green i y DN Run or Run Preview button Press Sherwood 140 ml 38 4 mm the Method Delay button clack Method Main screen on paper to set a delay sbowing a sample Metbod with 10 steps Delay 0 m c Ramp Constant Rate io oro gt us Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 32 SYRINGE SELECTION In order to accurately control the flow rate or volume delivery of the pu
124. omputer via USB the Found New Hardware Wizard will launch and ask how you wish to locate the driver Choose No not this time and click Next Found New Hardware Wizard This wizard helps you install software for Syringe Pure y Es If your hardware came with an installation CD 42 or floppy disk insert it now What do you want the wizard to do O Install from a list or specific location Advanced Click Next to continue Choose Install from a list or specific location Advanced and click Next Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 110 4 de Publication 5419 002 Rev E Found New Hardware Wizard Please choose your search and installation options PHDUltra w2k inf Hardware Installation The software you are installing For this hardware Syringe Pump Pista has not passed Windows Logo te gc with Windows lt P Tell me why this testing is import vartant Continuing your installation of this software may impair or destabilize the correct operation of pour system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for J Syringe Pump XP Vista Click Finish to close th
125. on 0 10v T 70 3034 PHD ULTRA Internal Fan Option 70 3035 PHD ULTRA Digital I O Option Only available with IAW and Programmable Models Only available with Programmable Models T Not available on satellite boxes Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Accessories Catalog 70 4000 70 4021 70 4001 70 4020 70 4002 70 4003 70 4004 55 7760 72 2478 5113 001 70 4014 70 4015 70 4005 70 4006 70 2215 55 7002 55 7004 2401 086 5012 005 70 4013 70 4019 Upgrades Catalog 70 4010 70 4011 70 4012 Appendices 151 Description RS 485 Cable for Pump to Pump Communication 0 5 m RS 485 Cable for Pump to Pump Communication 1 m RS 485 Cable for Pump to Pump Communication 2 m RS 485 Cable for Pump to Pump Communication 30 ft USB Cable for PC to Pump Communication 2 m USB Cable for PC to Pump Communication 5 m RS 232 Cable for PC to Pump Communication 9 pin D sub 2 m Cable Assy Daisy chain Legacy RS 232 RJ 11 2 ft Cable Assy Daisy chain Legacy RS 232 RJ 11 7 ft Line Cord US 115 v Cable for Analog Control Option Adapter Analog Cable to Terminal Block Adapter PHD Digital I O Adapter D sub 15 to Term Blk Footswitch w Phono Plug Auto Fill Valve Box Normal Pressure 30 psi Auto Fill Valve Box High Pressure 200 psi Adapter for 25ml 50ml 100ml Hamilton GasTight syringes Hex Key 3 32 Lubricant SuperLube 1cc Stand hol
126. onditions during pump operation The Message Area is displayed in yellow when one of these warning messages is displayed An example of an informational warning is shown below HARVARD APPARA TUS PUER 04 28 09 11 43 30 AM Withdraw Rate 3 225 The Set Infusion and Witbdrawal Rates screen sbowing an out of range 2 setting condition in the Message Area Tbe informational warning is displayed in yellow ml min Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual EZ PRO Touch Screen Button Reference The following buttons are part of the PHD ULTRA software El E E3 EX os Z r00 08 E Publication 5419 002 Rev E Settings Access the settings menu used to adjust the following parameters Date and Time Audible Alarms Pusher Block Force Backlighting RS 232 Communications etc Fast Forward Advances the Pusher Block in the forward infuse direction Fast Reverse Advances the Pusher Block in the reverse withdraw direction Accept Used to accept the parameters settings on a screen and advance to next screen in the menu Cancel Used to cancel any changes entries on a screen and return to the previous screen in the menu Back Used to go back one screen Set Delay Parameters Used to set the date and time values for delayed operation This permits the pump to start automatically at a preset date and time Set Delay Parameters Indicates the Delay Tim
127. ormerly Popper amp Sons Stainless Steel Size Diameter 2 5 mi 4 851 mm 8 9 525 20 19 13 50 28 6 100 34 9 Air Tite HSW Norm Ject Size Diameter 4 69 mm 9 65 12 45 15 9 20 05 22 9 29 2 1 ml Diameter 100 0 343 mm 0 485 0 728 1 03 1 457 2 303 3 257 4 606 mm 7 284 10 301 14 567 23 MICRO MATE Glass Size Diameter 0 25ml 3 47 mm 0 5 3 62 4 82 8 91 8 91 11 71 14 65 19 56 22 7 28 02 35 7 ml Diameter 6 50 mm 9 10 10 00 12 60 15 10 20 45 22 50 25 60 34 00 27 5 35 Becton Dichinson Plasti pak Diameter 4 699 mm 8 585 11 989 14 427 19 05 21 59 26 594 26 594 Terumo Diameter 4 70 mm 8 95 mm 13 15 8 20 15 23 1 29 1 Hamilton Glass All Types Size 0 5 pl Diameter 0 103 mm 0 146 0 206 0 343 0 485 0 729 1 03 Becton Dickinson Glass All Types i Diameter 4 64 mm 4 64 8 66 11 86 14 34 19 13 22 7 28 6 34 9 Natsume Size 0 25 ml 0 50 Diameter 2 5 mm 1 457 2 304 3 256 4 608 mm 5 151 7 285 10 3 14 567 23 033 32 573 32 573 Sherwood Monoject Plastic Diameter 4 674 mm 8 865 12 600 15 621 20 142 23 571 26 568 37 948 Diameter 6 40 mm 9 30 13 10 15 3 21 0 23 0 29 0 Suggested Force Level Settings for Common Syringes NIPRO Size Diameter 1 ml short 6 6 mm 1 mllong 4 7 2 5 ml 9 0 13 0 15 8 20 1 23 2 29 1 Syrin
128. ote system Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 126 APPENDIX H REMOTE STAND The Remote Stand accessory allows the user to maximize bench space by mounting two satellite remote or PC controlled units with a Master Stand Alone pump at the stand s base DIN Rail Satellite 2 Tilt Adjust Handles Satellite 1 Pump Rest Master Adjusting DIN Rail Height 1 Loosen both Height Adjust Knobs located on the sides of the Pump Rest 2 Slide the Pump Rest to your desired height 3 Tighten both Height Adjust Knobs securely NOTE If adjusting the height with a pump attached loosen one Height Adjust Knob By then use one hand to support the pump while loosening the second Height Adjust Knob NOTE Slot covers provided as stops to prevent the Pump Rests from colliding are Ge located in the slots on the side of the stand The stops can be trimmed to provide more traveling room for the Pump Rests To trim pop out the cover using a screw driver cut to desired length with scissors then snap back into place Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 127 DIN Rail Grooves for Locking DIN Rail Spring j Mounting Locking Spring Hight Adjust Knob Tilt Adjust Handle Adjusting DIN Rail Tilt 1 Loosen both Tilt Adjust handles by turning clockwise To increase the handles ran
129. ovide all operational and program information required to operate and maintain the PHD ULTRA series pumps The functions and features are described in the Technical Specifications section Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original consumer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 using our toll free number 1 800 272 2775 or outside the U S call 508 893 8999 Email Address is bioscience harvardapparatus com Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service depa
130. ower loss is lost Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA SYRINGE LOADING Helease Tabs Pusher Block Syringe Holder Block p e s o Retaining Bracket Pusher Block 5 Retaining Bracket Syringe Holder Block 6 Syringe Barrel Clamp Knob 7 8 Bracket Clamping Knobs 9 Mechanical Stop Infuse 10 Mechanical Stop Withdraw 11 Pulley Cover Allen Wrench Holder 12 Syringe Barrel Clamp Front view of PHD ULTRA sbowing important controls for syringe loading Adjust the Pusher Block 2 to the approximate length of the syringe s by squeezing the Release Tabs 1 and sliding the Pusher Block 2 into position Loosen the Retaining Brackets 4 5 on both the Pusher Block 2 and Syringe Holder Block 3 by unscrewing the Bracket Clamping Knobs 7 8 Lift the Syringe Barrel Clamp 12 by unscrewing the Syringe Barrel Clamp Knob 6 Place the syringe s on the Syringe Holder Block 3 Ensure that the barrel flange is within the Syringe Holder Block s Retaining Bracket 5 and the plunger flange is within the Pusher Block s Retaining Bracket 4 Close up of syringe positioning witb plunger inserted in retaining bracket of pusher block Lower the Syringe Barrel Clamp 12 by screwing down the Syringe Clamp Knob 6 until the clamp is tight against the syringe barrel Push the Retaining Brackets 4 5 tightly against the syr
131. p to 100 from a single computer interface PHD ULTRA commands are communicated to the pump via the RS 232 or USB port interfaces through a terminal program such as HyperTerminal In using the Pump Chain commands you will need to assign each pump in the pump chain a unique address using the Set Pump Address command in the Settings menu The address range is from 00 to 99 This address value is used to identify which pump is to receive a command and which pump is responding The first pump in the chain the one connected to the computer must use address 0 zero Configure each pump with its assigned address and baud rate as described in the Pump Settings section of this Guide NOTE Once communication is established if you manipulate the touchscreen you must reestablish communication CR hs NOTE When daisy chaining using the RJ 11 option all pumps must use the same baud rate Values above 38400 are recommended ds NOTE If you are using the optional RJ 11 connections for daisy chaining the connection from the computer must also come through the RJ11 connectors ds NOTE System commands and start stop commands can be executed from method or Quickstart screens For parameter commands it is necessary to run from Quickstart screen hs Using PHD ULTRA Pump Chain Commands Commands may be abbreviated to the first four letters i e address would be abbreviated addr A space must follow the command if arguments are included
132. password defined by the user Change Logo Upgrade Software Audible Alarms Pump Information Baud Rate 115700 Address O Power Up Running Quick Start Infuse Withdraw BD plastic 60 ml 26 59 mm 75 ml min 88 28 ml min EXE ii Method Select Quick Start Infuse Withdraw Syringe Select BD plastic 60 ml 26 59 mm Infuse Rate Select 75 ml min Withdraw Rate Select 88 28 ml min HARVARD APPARA TUS 10 27 09 12 00 05 PM Select the desired setting and then press the Enter button green check mark to accept or press the Cancel button red X to return to the previous screen HARVARD APPARA TUS Enter the settings Make sure to set a target volume gm ale HARVARD APPARA TUS 01 21 10 09 34 37 AM Enter the Passweord up to E digits L1 HARVARD APPARA TUS 04 28 09 12 21 28 PM The screen is lacked Press the lock button to unlock it Target Volume Select Lj 60 ml SETTING THE PASSWORD To set the Password go to the Settings screen Press the Set Password button Enter your desired password up to 8 digits and press the enter button TURNING THE TOUCHSCREEN LOCK ON Press the Lock button twice to lock the touchscreen Methods can be started and stopped while the touchscreen is locked Method parameters can not be changed while the touchscreen is locked Password Lock Button TURNING THE TOUCHSCREEN LOCK OFF Press the Lock button to b
133. played in the Method Select button verify that the proper syringe information is set The currently defined syringe settings are displayed in the Syringe Select button If the syringe information is not correct follow the procedure for selecting a syringe defined previously in the Syringe Selection section of this manual Verify that the Rate and Volume Time settings are properly set If changes are required refer to the procedures described later in the Quick Start Method Variables section of this manual With all Method parameters set as desired check to see if the Run button is active If any required parameters are not set or are improperly set e g a value is out of range based on syringe selection or other condition the Run button will be inactive grayed out You must have all required parameters validly defined before the Method can be started Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual PREVIEW THE METHOD s HARVARD APPARA TUS change Current settings 55 15 09 1 29 18 PM Infuse Withdraw SGE 1 03 mm 50 00 ul Target 50 ul Infuse 158 984 ul min Withdraw 156 984 ul min l 5 ee le LE 0 000 ul Time Elapsed 00 00 00 Time Remaining 00 00 36 Total Inf Volume O ul Force Level 100 1 step s Force Level 100 SGE 50 ul 1 03 mm Time Elapsed 00 00 06 Total Volume Dispensed 3 981 ul Ready to infuse Press the green Run button to start the infusio
134. pump address is nonzero the pump address is prefixed to the prompt without a colon In the following list of commands The pump address is prefixed to every response line followed by a colon It prompt is the response unless otherwise noted Error messages Error messages are displayed if the entered command cannot be executed for some reason The error message will take up two lines with the first line being the message type and the second line describing the error itself The second line may be up to 80 characters long COMMAND ERRORS Command errors are displayed when the command is unrecognized entered in the wrong mode or the state of the pump keeps the command from executing i e using the IRUN command if a limit switch is active The command error has the following format lt If gt Command error lt cr gt It sp sp sp error message lt cr gt It prompt ARGUMENT ERRORS Argument errors are displayed when a command argument is unrecognized or out of range The argument in question will be displayed except in the case of missing arguments The argument error has the following format If Argument error bad argument cr lt lf gt lt sp gt lt sp gt lt sp gt error message cr If prompt Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA System commands ADDRE
135. r OO al octne the Tie and M Dj ce NNNM Um 22 VE SIs e gsc suet es 22 na ld AA on 23 SU Pamp Address A o Pe ia aAA E 23 Sete hie Pump Data Rate uranio 24 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Table of Contents Pomp ot DEAD AAA NE Oo o fone Med NUI I Me RT EIE UU UUEE 25 Power Up RONGO e cO 26 E un 2 Ghaneine the bracket for small 50 m D vs large 50ml SVIDPEST assaisonner 28 Using The PHD ULTRA llle ere ILI LLL c erre eres crearan renacer ns sns nnmnnn nne 29 lio Ojo ue rra T 2 Over EE JVISEDOUS ica 29 lA E E A E O E ee 30 LAPRO Oac Stt NANOS eaan n a cata 30 EZ PRO Preloaded Met HOGS sinnnrnssnntaaaiadiaicda 30 EZ PRO User Dolined Methods anabel 30 Pannor Tho D UN o OOOO OEI Cuero E E E errr TT terre ter cree oe 31 Conroe Toe TU eee AEE E EA EAEE EEE E A EEA Jl Basie ED EE a asian A E A E A E ree ent rere eee 31 Miesung Current Meihod Parmele esinaine ai UM m iU 3l a E E 0 ANUN II E A T E E EE 2 ayunas Gano occ irons 35 Controlling The Pump Usina A Stoted Method eur 35 Sri Ai EZPRO Quick tute Metodos 39 pecu sociae aao a oo A aU Quick start Meihod Vaart biota 40 Infuse Rate Select Withdraw Rate Select is 40 Taroet Volume Bins ceo cll CE emos 41 Using an EZ PRO Preloaded Uset Defined Method eene 42 Preloaded User Defined Method Workflow
136. r methods periodically by uploading them to a PC Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 121 Upgrading EZ PRO Software 1 Upload the latest software version to your desktop format is filename dfu 2 Connect the pump to PC using a USB cable 3 Press settings button then press Upgrade Software button twice The pump s screen will be blank and the light should be flashing Y NOTE To exit bootloader mode without upgrading the software power cycle the pump NOTE First time users will need to install the PHD ULTRA USB driver as well as Y the bootloader driver file PHDUltra Bootloader inf Reference USB Virtual Comport Driver Installation 4 Disconnect then reconnect the USB cable 5 From the CD provided with the pump open the PHD ULTRA Flash Upgrade Application ime Pump Flash Upgrade Application Select Pump To Upgrade File PHO Ultra v1 1 4 dfu Verify after download Optimize Upgrade duration Remove some FFs File correctly loaded 6 Check Verify after download then click browse Browse to the file previously saved on your desktop and click open 7 Click upgrade The previous software will first be erased the new software will be loaded and finally the software will be verified 8 Disconnect the USB cable and power cycle the pump HARVARD APPARATUS PHD ULTRA PUMP TYPE INFUSE WITHDR AW PROGRAMMABLE Pump firmw
137. reen are correct choose the Accept button to return to the Step Definition screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 62 GRADIENT PROFILE Available in firmware v1 2 2 and later The Gradient profile is used to combine multiple flow streams from different pumps into a common output stream The flow profiles can be flat of ramped smooth flowing or can execute in a stepped format Gradient Delivery Pump Operation Profile When creating or editing a Gradient profile you will define 4 primary operating parameters The combined Flow Rate e The Target Starting and Ending Percentages for each pump in the system e TheTarget Time that determines the total length of time over which the Gradient profile will be calculated e The Number of Steps that will be created in the profile NOTE See Appendix E External Connections for instructions on setting up a Gradient System and controlling satellite pumps NOTE Master ADDRESS must be set at 00 Satellite ADDRESSES range from 01 to 99 and must be set thru USB While the actual satellite s address is not zero ensure that the Remote Address field under the settings screen is set to 0 when running a gradient step e e CAUTION All pumps must use the same syringe size and type If there is a syringe change at Master then same change must occur at all Satellites 1 CAUTION For firmware versions prior to 1 3 0
138. ring up the Enter the Pump Password screen Enter your password and press the Accept button NOTE If you forget your password contact Harvard Apparatus Technical Support at 508 893 8999 Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 21 Setting the Force Limits The PHD ULTRA motor generates up to 75 Ibs of linear force in operation This force is sufficient to damage delicate low volume syringes as well as many standard syringes if pumping viscous fluids The application software provides controls to allow you to set a maximum force value calculated as a percentage of the maximum value HARVARD APPARA TUS 04 28 09 11 51 09 AM Select an existing percentage or select set other value and then press the Enter button green check mark to accept or press the Cancel button red X to return to the previous screen fO o 20 NOTE For firmware versions prior to 1 3 0 when Satellite units are connected to Master Force Setting only affects force limit on Master Satellites retain last set value Default force 50 TO ADJUST THE MAXIMUM FORCE SETTING 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu choose the Force button to enter the Set Force Level screen 3 Select the desired preset level value or use the Set Other Value button to enter a custom force level 4 If you select S
139. rm your settings and return to the Step Definition screen Choose the Set Percentages button for pump 00 Set the Start and End Percentages for pump 00 then choose Accept Choose the Address button and then set the address of the second pump Choose Accept to return to the Step Definition screen HARVARD APPARATUS Pump number o 10 27 09 11 55 17 AM Enter the number of the pump and then press the Enter button green check mark to accept or press the Cancel button red X f 9 to cancel your settings and return Gradient Pump Address E E O to the previous screen Entry Screen 9 Choose the Set Percentages button for the second pump and enter your desired percentage then press the accept button 10 Choose the second Address button and then set the address of the third pump Choose Accept to return to the Step Definition screen 11 The pump will calculate the remaining percentages for the third pump NOTE If performing binary gradients you do not have to set the address for the third Y pump Verify that the calculated values for this non existent pump are equal to zero NOTE To unassign a pump from a gradient step set pump address to zero in the Set Pump Number screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 64 12 Choose the Set Time amp Steps button Enter the total amount of time and the number of steps desired and then choose Ac
140. rofile follow the instructions supplied in the applicable Pump Operation Profile description contained in the Setting Method Parameters section later in this Guide 8 To return to the Method Steps Summary screen when finished reviewing or editing the Step parameter details choose the Accept button to save your changes or choose the Cancel button to cancel any changes you made 9 When you are finished reviewing the operating details for all Steps choose the Back button to return to the Method Main screen 10 To preview the Method choose the Preview button or to run the Method choose the Run button Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting A Time Delay For Method Operation Using The PHD ULTRA 45 You can set the PHD ULTRA to run a Method at a specific date and time in the future This delayed run function allows you to set up all the parameters for operation then have the pump start unattended at the required time Quick Start Infuse Withdraw BD plastic 50 ml 26 594 mm HARVARD APPARA TUS 10 28 09 11 45 17 AM Enter your settings to the left and then press the green Run ar Run Preview button when finished Make sure to set a target volume ETS E JARA panem ed 2172103 METHOD Sherwood 140 ml 38 3 mm Delay Ramp Constant Rate HARVARD APPARATUS 10 27 09 10 14 46 AM Select the desired syringe define steps and then press the green Run
141. rtment The customer is responsible for shipping charges for non warranty repairs Please allow a reasonable period of time for completion of repairs or replacement If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using a part number obtained from our Technical Support department If practical enclose a sample part or sketch We offer a complete reconditioning service Serial Numbers All inquiries concerning our product should refer to the serial number of the unit located on the rear panel Calibrations All electrical apparatus are calibrated at rated voltage and frequency While the flow and volume will stay calibrated the peak pressure may vary Harvard Apparatus recommends an annual calibration of the pump N CAUTION For research use only Not for clinical use on patients Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 8 General Information SAFETY INFORMATION Please read the following safety precautions to ensure proper use of your syringe pump If the equipment is used in a manner not specified the protect
142. s and return to the Settings screen NOTE To reset the Logo value back to Harvard Apparatus backspace in the Change Logo text entry box until the field is blank then choose Accept Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 23 Setting Backlight Level You can adjust the backlighting level on the PHD ULTRA touchscreen to optimize viewing in your specific lighting conditions On the Settings screen the Backlight button displays the current setting on the button HARVARD APPARA TUS 04 28 09 11 53 05 AM Select an existing percentage ar select set other value and then press the Enter button green check mark ta accept ar press the Cancel button red X to Set Backlight Level screen return to the previous screen 70 o 20 TO MODIFY THE BACKLIGHT LEVEL 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu choose the Backlight button to enter the Set Backlight Level screen 3 Select the desired preset level value or use the Set Other Value button to enter a custom level 4 If you select Set Other value enter the desired setting as a percentage value 10 100 then choose Accept to confirm your setting 5 When you have selected or entered the desired backlighting value choose Accept to save your changes and return to the Settings menu Setting Pump Address Value The Pump Address value
143. s difficult to manage the connection and disconnection of devices Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal NOTE 1 Satellite and Remote units are DC powered through the RS 485 cable Earth ground connection is through this cable for these units Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 9 Introduction To The PHD ULTRA 10 Introduction To The PHD ULTRA Pump PRODUCT OVERVIEW THEORY OF OPERATION The PHD ULTRA Syringe Pump series is a family of high accuracy microliter and milliliter compatible pumps designed for versatile technical use including mass spectroscopy calibration drug and nutritional infusions microdialysis dispensing chromatography and LC HPLC The PHD ULTRA incorporates a microprocessor controlled small step angle stepping motor that drives a lead screw and Pusher Block Advanced micro stepping techniques are employed to further reduce the step angle to eliminate flow pulsation The pump is engineered to provide flow accuracy within 0 35 6 and reproducibility within 0 05 The PHD ULTRA uses a touchscreen interface and advanced software to control operation External I O interfaces permit external control via an independent computer or
144. screen go to the Settings menu 2 Press the Address O Remote X button HARVARD APPARA TUS Upgrade 03 30 10 02 03 28 PM oftware Change Logo Select the desired settings Audible Pump Alarms Information Settings screen showing r 3 Baud Rate the Address button 115200 Address O Remote 1 Set Password Running g E Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 117 3 Setthe Pump Address to O 4 Press Remote Address and enter the address of the remote unit 5 Press Accept HARVARD APPARA TUS 03 30 10 02 06 42 PM Enter a value between D and 99 Set Pump Address screen Pump Address Remote address NN E 6 E L1 NOTE For gradient systems the Remote Address must also be set to O in the Settings A menu Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices APPENDIX F MAINTENANCE Maintenance PHD ULTRA series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material Refer to the chemical compatibility chart below for specific chemical resistance information The two guide rods and the lead screw should be sparingly lubricated periodically every 200 hrs the pump will remind you The guide rods and the lead screw should be lubricated with Super Lube Synthetic Grease provided with the pump To clean the ext
145. sed to display the pump operations method information general parameter settings pump name the time date display and the message Y and the real time display of data while the pump is running y box which contains operational messages while the pump is running HARVARD APPARATUS Run Preview Access the Method Preview 10 27 09 11 31 07 PM all screen that shows a summary of steps and Quick Start Infuse Withdraw operating parameters Enter your settings to the left Run Resume Used to Run or Resume a and then press the green Run or gt Method acti Run Preview button when BD plastic 50 ml 26 594 mm finished Make sure to set a Fast Forward Advances the Pusher Block target volume in the forward infuse direction Fast Reverse Advances the Pusher Block in the reverse withdraw direction Back Used to go back one screen Na Touch Screen Unlocked Indicates that the Touch Screen is currently unlocked Pressing the button twice locks the Touch Screen A Settings Access the settings menu used to adjust Date Time Audible Alarm Pusher Block Force Backlighting etc Navigation Buttons Set Delay Parameters Used to set the date this area of the touchscreen contains the and time values for delayed operation This buttons used to navigate through the software permits the pump to start automatically on menus and to start stop pump operations the date time selected 1 Navigation Buttons
146. spensing scenarios including e Multiple infusions e Ramped or stepped infusion rates e Periodic dispensing loops e Combination infuse and withdraw profiles e External system control or response to external signals Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 30 METHOD TYPES EZ PRO Quick Start EZ PRO Quick Start Methods are designed for simple infusion or withdrawal applications pumping at a constant flow rate for either a predetermined time period until a preset volume is pumped or running in continuous operation until stopped The PHD ULTRA is supplied with the following pre installed Quick Start Methods depending on the model of PHD ULTRA pump you are using Infuse Only All Models The Infuse Only Quick Start Method is designed to pump liquids from the syringe at a set flow rate Users can set a Target Volume or Time period for operation If no Target Volume or Time value is entered the pump runs continuously until the Mechanical Stop is reached or the user presses the Method Stop button e Withdraw Only Infusion Withdrawal and Infusion Withdrawal Programmable Models The Withdraw Only Quick Start Method is designed to withdraw liquids into the syringe at a set flow rate Users can set a Target Volume or Time period for operation If no Target Volume or Time value is entered the pump runs continuously until the Mechanical Stop is reached or
147. ss all types green check mark to accept or press the Cancel button red X Plasti pak to return to the previous screen Syringe Selection screen with currently selected Micro Mate Glass syringe Glass all types Stainless Steel 2 From the Syringe Selection menu double tap the Manufacturer Model button for the desired syringe to enter the Syringe Size Selection screen HARVARD APPARATUS AOT 04 28 09 12 22 56 PM Select the desired syringe size aa TID 2 5 ml and then press Enter button green check mark to accept or Syringe Size Selection 9 525mm 8ml press the Cancel button red X screen sbowing barrel to return to the previous screen dimensions and syringe 19 13 mm 20 ml capacity 28 6 mm 50 ml 34 9 mm 100 mi 3 Double tap the dimensions capacity button for the syringe you will be using Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 33 TO CREATE A CUSTOM SYRINGE OPTION 1 From the Quick Start or Method Main screen choose the Syringe Select button 2 From the Syringe Selection menu double tap the Custom Syringe option at the top of the syringe selection menu 3 On the Syringe Dimension Entry screen the Diameter mm entry box should be highlighted by default If it is not choose the Diameter mm button HARVARD APPARA TUS 04 28 09 12 23 50 PM Enter your settings to the left and then press the Enter button green ch
148. tart mode only Command format wramp start rate end rate rate units ramp time in seconds Query response If Ramp not set up lt cr gt If prompt Of If Starting rate xl xxx lt cr gt If Ending rate xl xxx lt cr gt If 4 Ramp time seconds lt cr gt If prompt Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA RATE COMMANDS CONTINUED WRATE Sets or displays the withdraw rate Quick Start mode only Command format wrate rate rate units max min Query response It xI xxx lt cr gt Ift prompt Y NOTE For commands requiring rate units use the following format m u n p h m S Ex m m milliliter minute n s nanoliter second VOLUME COMMANDS CIVOLUME Clears the infused volume Quick Start mode only Command format civolume CTVOLUME Clears the target volume Quick Start mode only Command format ctvolume CVOLUME Clears both the infused and withdrawn volumes Quick Start mode only Command format cvolume CWVOLUME Clears the withdrawn volume Quick Start mode only Command format cwvolume Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 89 Using The PHD ULTRA VOLUME COMMANDS CONTINUED IVOLUME Displays the infused volume Quick Start mode only Comman
149. te Format button to the right of the Date entry box You can toggle between m d y Month Day Year d m y Day Month Year and y m d Year Month day display by successive selection of the Date Units button To change the Time select the Time button and enter the current time in hh mm ss hours minutes seconds format Select the Time Format button to toggle between AM 24 Hour and PM values for the Time When the Date and Time values are correct choose the Accept button to save your changes and return to the Settings screen Change Logo The PHD ULTRA displays a descriptive name termed logo in the application on the screen The default value for the logo is Harvard Apparatus This name is editable by the user to allow you to customize the pump for your department organization TO MODIFY THE PUMP LOGO 1 2 2 From the Quick Start or Method Main screen choose the Settings button On the Settings screen choose the Change Logo box to enter the Change Logo screen Using the onscreen keyboard type the name that you want to have displayed The display is limited to 15 characters HEN 15 CHAR MAX o w e r Tv o P 8 18 1294 15818 CO e E ZEEN m Eras sace OO To type numbers or symbols select the 123 button to toggle the keyboard to numbers symbols mode To return to letters mode press the ABC button When finished entering the new name choose the Accept button to save your change
150. tered choose the Accept button to save your Changes and return to the Method Main screen When a Time Delay value is active the Time Delay Parameters button is displayed in Red on the screen to alert the user that a delay has been set 5 Press the Run button to activate the Delay HARVARD APPARA TUS crne NN 10 28 09 11 52 21 AM Infuse Withdraw Et pr eee UU SN Pump will run at the following epu Sl mi a date and time 10 28 09 Infuse 5 ml min 12 00 00 PM Press withdraw 5 ml min e DEA ent button to cancel delay Quick Start metbod witb a Flow Direction EG PRESS TO CLEAR MESSAGE Time Delay set pc SEES 00 00 00 Time Elapsed Time Remaining 00 20 00 Total Inf Volume O ml e 4 O Force Level 100 CREATING AN EZ PRO NEW METHOD If none of the Methods stored on the PHD ULTRA match your requirements you can create a new Method and store the Method for use The following flow diagram outlines the basic steps you will follow to create a new Method Choose Syringe Select Screen Method Main Syringe Select Y Choose Step Definition Y Method Selection Method Steps Summary Screen y Add Step y New Method Step Definition Screen New User Defined Method creation workflow Complete Parameter Entry Choose Accept Add More Steps Name Method Steps Summary Screen
151. the Enter button Start D ml min End 0 ml min green check mark to accept or press the Cancel button red X to return to the previous screen Steppe d Profile definition screen D secondis D Step s HARVARD APPARA TUS Start Rate o eec 04 28 09 1 24 36 PM Enter your rates and units and DIRE JO then press the Enter button green check mark to accept or E rN press the Cancel button red X Set Start and End Rate screen to cancel your settings and return 105 985 to the previous screen El E 6 mil min nil min 3 Select the Start Rate button it should be selected by default as indicated by the active data entry box to the right of the button Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button 4 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections Repeat Steps 3 to 5 for the End Rate by selecting the End Rate button and continuing data entry When all parameters are correctly set on the Set Rate screen choose Accept to confirm your settings and return to Step Definition screen Choose the Set Time and Steps button to enter the Set Time amp Steps screen Select the Total Time button it should be selected by default as indicated by the active data entry box to
152. the preceding step choose the Set Time or the Set Volume button to enter the Set Pulse Times or the Set Pulse Volumes screens HARVARD APPARA TUS deco me Enter your times and then press E the Enter button green check mark to accept or press the Set Pulse Times screen used E Cancel button red X to cancel defi he 1 p your settings and return to the to define the length of time previous screen the pump will operate at 4 E 6 each pulse step level If you selected Set Time continue with Step 11 If you selected Set Volume proceed to Step 14 On the Set Pulse Times screen select the Time 1 button it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired value using the keypad Repeat for Time 2 If the Units of Time associated with Time 1 orTime 2 are not correct for your application select the Units button associated with either parameter to toggle between sec Seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon When both Time parameters are set to the correct values choose the Accept button to return to the Step Definition screen On the Set Pulse Volumes screen select the Volume 1 button Gt should be selected by default as indicated by the active data entry box to the right of the button Enter the desired value using the keypad Repeat for Volume 2
153. the right of the button Enter the desired value using the keypad 10 If the Units of Time are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 11 Select the Number of Steps button and enter the desired value using the keypad 12 When all parameters are correctly set on the Set Target Time screen choose Accept to confirm your settings and return to the Step Definition screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 56 PULSE PROFILE The Pulse Flow profile is used to create an oscillating infusion or withdrawal profile that varies between a starting flow rate and a second rate that can be either higher or lower than the start rate The flow rate is constant within each segment of the pulsed flow profile and the user defines the total number of repetitions pulses for the oscillating pattern Each segment of the profile must be set to run for either a specific length of time or to deliver a set volume Pulses Pulse Flow Pump Operation Profile Time Volume When creating or editing a Pulse Flow profile you will define four primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw The Flow Rate to be used at each level of the p
154. the user presses the Method Stop button e Infuse Withdraw Sequence Infusion Withdrawal and Infusion Withdrawal Programmable Models The Infuse Withdraw Quick Start Method is designed to cycle through an infusion operation followed by a withdraw operation The user can set separate flow rates for both the infusion and withdrawal sequences In addition the user must set a Target Volume for the Method e Withdraw Infuse Sequence Infusion Withdrawal and Infusion Withdrawal Programmable Models The Withdraw Infuse Quick Start Method is designed to cycle through a withdraw operation followed by an infusion operation The user can set separate flow rates for both the withdrawal and infuse sequences In addition the user must set a Target Volume for the Method EZ PRO Preloaded Preloaded Methods have been designed by Harvard Apparatus engineers to accommodate commonly employed pump control delivery protocols These applications include infuse withdraw and combined infuse and withdraw protocols Preloaded Methods can be edited on Infusion Withdrawal and Infusion Withdrawal Programmable models of the PHD ULTRA They are not available on the Infuse Only model The Preloaded Methods are continuously updated by Harvard Apparatus Engineers so the Methods installed on different pumps may vary For details on specific use of these Methods refer to the applicable instructions in the Quick Start or User Defined Methods sections of this manual E
155. ue address Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 125 Setting Addresses on Satellite Remote Units 1 Connect Satellite Remote unit to PC via a USB cable 2 Launch HyperTerminal 3 Set address using the appropriate Pump Chain Commands Setting Forces on Satellite Remote Units 1 Connect Satellite Remote unit to PC via a USB cable 2 Launch HyperTerminal 3 Setforce to desired level in percent using the appropriate Pump Chain Commands NOTE For software versions prior to 1 3 0 the Force level needs to be set from a PC Y via a USB cable With software versions 1 3 0 and newer the Force Level will be sent down to the remote satellite units directly from the master Optional PC to Pump Configurations POWER STAND ALONE PC CONTROLLED PLIMP ADOR 1 USB POWER STAND ALONE OF PO CONTROLLED PLIMP ADDR 02 STAND ALONE PC CONTROLLED PLIMP POWER ADDR n2 STAND ALONE PC CONTROLLED PLIMP ADOR 04 ADDRESSES CAM BE 01 99 POWER PC Control configuration option CONTROL BOX 1 RS 485 REMOTE HE SIDES SATELLITE k ADDR 01 OPTIONAL R 5 485 R5 485 OUT IH CONTROL BOX n R 485 REMOTE _ SATELLITE AOS ADDR 01 RS 485 RS 485 OUT IH PC Control configuration option Y NOTE Control Box must be set at address 0 Y NOTE Unique comports must be selected for each rem
156. uide rods and lead screw are properly lubricated RS 232 Difficulties Verify that the baud rates data framing parameters data bits parity and stop bits on all devices are the same Verify flow control handshaking is set to None If an address is set to other than address make sure commands and queries are prefixed with the address Make sure straight through pinning is used for the cables do not use null modem cables Erase Clear Disc There may be an instance where you want to delete all of your custom methods from the pump There may also be instances where you may need to delete clear the disc in order to power up the pump To do so e Turn the pump on When the start up screen appears press the 4 corners of the display in a clockwise order starting at the top left After each corner is correctly pressed it will turn dark gray e Press yes to confirm or wait 5 seconds to allow the pump to start normally without clearing the disc e There is a 5 second time limit for each press of the screen If the time limit expires the pump will start normally without clearing the disc HARVARD APPARATUS PHD ULTRA Press the 4 corners of the start up screen display in a clockwise order to delete clear the disc PUMP TYPE INFUSE WITHDRAYW PROGRAMMABLE Pump firmware v1 3 1 RTOS FreeRTOS v6 0 1 http www freertos org ap address O r Disk space free 790 method steps 1 ZB NOTE It is recommended that you save you
157. ulsed profile within the pumping cycle The length of Time that each segment of the profile will run or the specific Volume to be infused withdrawn during each segment of the profile The Number of Pulses repetitions that will be executed during the pumping cycle During a Pulse Flow profile the PHD ULTRA calculates a series of equal length pumping stages at two user defined flow rates a starting rate and a pulse rate You enter the Number of Pulses repetitions that you want to use along with how you want the system to calculate the length of each stage by time or by volume pumped A Pulse Rate profile can be applied to pulses that are higher in rate or lower in rate than the starting flow rate When creating or editing a Pulse Flow profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Pulse Rates screens HARVARD APPARA TUS Enter your settings to the left and then press the Enter button a Rate 1 0 ml min Rate 2 0 ml min green check mark to accept or Step Definition screen for a press the Cancel button red X Pulse Rate Profile with to return to the previous screen Volume mode selected D ml a ml D Pulse s Publication 5419 002 Rev E Harvard Apparatus PH
158. un is completed Touch anywhere on touchscreen to silence the alarm e Power Up An alarm will sound if power to the PHD ULTRA is lost while the pump is running Pressing any button on the screen silences this alarm Motor Stall An alarm will sound to notify the operator of any motor stall e Keypad Clicks A soft beep indicates a positive touch on any button on the screen Near End of Run This alarm 3 beeps can be custom configured to notify the operator that a Method is near completion The user can select from preset values of 75 80 85 90 or 95 completion or can set a custom value using the touchscreen e Calibration Reminder This alarm is used to inform the user that the system has reached its scheduled Calibration date It is checked at each power up Mute All Selecting this option mutes all audible alarms When selected the Mute All button is indicated with a checkmark and the remaining buttons on the screen are greyed out HARVARD APPARA TUS 04 28 09 11 45 30 AM Select the desired alarmis and then press the Enter button Set Audible Alarms screen green check mark or press the Cancel button red X to return to the previous screen showing the available options and with Mute All selected The remaining alarm options are greyed out in this example Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 18 Setting Up The PHD ULTRA 19 TO SET THE USER CONFIGURAB
159. uttons used to navigate through the software menus and The PHD ULTRA Touch Screen Display The screen shown is the Quick Start display Quick Start Infuse Only The PHD ULTRA touchscreen with EZ PRO Software allows you to navigate through all menu selections and data entry by gently touching the onscreen buttons with a finger or the tip of a soft non sharp object such as a pencil eraser The software is organized into three main navigational branches e Quick Start Operations Preloaded User Defined Methods e Systems Settings The Method Main or Quick Start screens are the primary home for the applications From those screens you can access all the commands needed to operate the PHD ULTRA as well as the main system settings From anywhere in the applications you can return to the Quick Start or Method Main screens by e Choosing the Cancel or Home Back buttons one or more times until you reach the Method Main or Quick Start screens e Choosing Method Select and making a selection from the Method options available Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 15 The PHD ULTRA supports touchscreen orientation auto rotation landscape portrait for permitting easy use of the pump when positioned upright on its side This configuration and the associated touchscreen display are shown in the following figure Quick Start Infuse Onl
160. y Sherwood 38 4 mm 140 ml 220 975 ml min HARVARD APPARATUS 05 05 09 4 23 55 PM Enter your settings to the left and press the RUN or Run Preview button Make sure to select the appropriate syringe pe liaa p gt Eu P Tbe PHD ULTRA pump sbown in its vertical uprigbt orientation and tbe associated Quick Start menu screen sbowing tbe application auto rotated into portrait mode SELECTING AN ITEM FROM A MENU LIST Some sections of the PHD ULTRA software display a list of choices from which you select These menus require that you first select the item that you want When you do this the item you have selected is highlighted in magenta on the screen To confirm your selection you must choose the Accept button or touch the item a second time An example of the two step selection process with a button color displayed in purple is shown below HARVARD APPARATUS 10 2709 11 37 51 PM Select an existing method or create a new method and then He AA The EZ PRO Method Selection screen showing a button highlighted after a single touch Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 16 INFORMATION WARNING MESSAGES The Message Area of the touchscreen is used to display helpful instructions for the currently displayed screen It is also used to display error or warning messages to indicate problem conditions in a Method or error c
161. your changes and return to the Quick Start screen Publication 5419 002 Rev E Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 40 Choose the Infuse Rate Select button to access the Set Infuse and Withdraw Rates screen Enter the required parameters on the Set Infuse and Withdraw Rates screen Details on setting the Infuse and Withdraw Rates are provided in the Quick Start Method Variables section of this Guide When finished choose the Accept button to save your changes or choose the Cancel button to cancel your changes and return to the Quick Start screen Choose the Target Volume Select button to enter the Set Target Volume screen Enter the required data on the Set Target Volume screen as described in the Quick Start Method Variables section of this Guide Quick Start Method Variables INFUSE RATE SELECT WITHDRAW RATE SELECT The Quick Start Infuse Rate and Withdraw Rate Select functions define the rate of pumping while infusing or withdrawing liquids respectively The two functions are controlled identically so we have described the operation here only once When adjusting the Infuse Rate you can set the rate in ml ul nl or pl units of volume and use sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse and

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