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Harvard Apparatus Pump 11 Elite

1. ES Ca Creating a New Custom Method sssee mme nenne 34 EE To Create a New User Defined Method ccc ccc eeccccnecceeeeeeeeeeeeeneeeeans 34 E Modifying an Existing User Defined Method seseeemmRHRR 36 L Changing the Order of Steps in a Method ssssssemHHRRe 36 a Deleting a Step from a Method sses ens 36 I Defining Method Step Parameters ssssssssse eee 36 P C Pump Operation Profiles astantes ted tin dROs Dev hustubs cet utinipei edades sU Ua aNE 37 I Constant Rate Profile 38 M SZT omae MEET TIE 39 EITS FOTW RN 40 VASE er re aro igo MEE 42 Incorporating Advanced Options Into a Method sssssseR 43 pc 44 Repeat P 44 vo 44 HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Table of Contents 4 SUBJECT PAGE NO VO aee RT 45 V O Trigger OUT 45 Het avd E E E E E EEA eenonctial 46 Managing Pump 11 Elite Series Vietnodsa RH 47 Savin a Copy Of a Method cessit toten utto arteo iaiaaeaia 47 i i UWS qii sees NER TOR 47 Appending a Method sssssssssssenm HII RI nm nnne 47 Deleting a Method e HI mnm nnnm
2. Enter the desired value using the keypad or select the Maximum Volume button If the Units of Volume Time are not correct for your application choose the Units button to the right of the parameter entry box For the Units of Volume this choice takes you to the Units Selection screen For the Units of Time option this choice will toggle the button between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon If you are changing the Units of Volume on the Units Selection screen select the unit of T 4 measure ml ul nl or pl as appropriate then choose ACCEPT to confirm your selection When all parameters are correctly set on the Set Target Volume or Time screen choose ACCEPT to confirm your settings and return to the Constant Rate Definition screen Choose ACCEPT again to return to the Step Definition screen phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Ramp Profile The Ramp Rate profile is used to create a linearly increasing or decreasing flow rate for a single pumping Method infuse or withdraw over the course of a defined time period Target Time Flow Rate Ko Time Ramp Rate Pump Operation Profile When creating
3. To enter the settings screen press EN web WWW harvardapparatus com Quick Start HARVARD APPARATUS Method Select Quick Start Infuse Withdraw Enter the settings Make sure to set a target volume Infuse Rate Select 75 ml min Withdraw Rate Select 88 28 ml min Target Volume Select 60 ml phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series 20 Setting the Audible Alarms Settings HARVARD APPARATUS In the Settings screen press Audible Alarms L Change Logo Software Select the desired settings Select the alarms a check mark will Audible Pump Press Alarms Information indicate the alarms selected Baud Rate Force 100 If these alarms are selected the pump 115200 will provide an audible alarm Backlight 10096 Address 0 TILT CC Accept the changes made by pressing aye and go back to the Settings screen iem LES EJ Cancel the changes made by pressing and go back to the Settings screen Power Up Alarm Keypad Clicks When the when pump is keypad is touched the unit power cycled while Will provide audible feedback running HARVARD APPARATUS End of Run Alarm when m Endof Run m Power Up the Method is finished Select the desired alarm s Stall Motor stalls u Stall m Keypad Clicks Near End of Run When the volume dis m Near End
4. event 3 Footswitch Input Momentary switch closure to ground or TTL Logic Low falling edge toggles between states run stop 4 Trigger 1 Output TTL Logic Output Default Low Control thru Run Indicator or Voltage Method or serial comm 9 13 Signal Return Ground User I 0 Connector Specifications 14 Run Indicator Voltage 5V through a 470 ohm resistor connect to LED Anode 15 Run Indicator Output TTL Logic Output active low low run connect to LED Cathode Electrical Specifications Inputs VIH Z 2V IH lt 20pA VIL lt 0 4V liL lt 0 5mA Outputs VOH 2 3 8V lOH lt 6mA VOL x 0 4V IOL lt 6mA E 72 EE EX n lt gt A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m 72 m E v2 lt gt A em gt KO HARVARD APPARATUS Utilizing the USB Interface Mating Face USB VIRTUAL COMMPORT DRIVER INSTALLATION When you connect the Pump 11 Elite Series pump to a computer via USB for the first time Windows will seek to install a driver for communication The following section details the installation of the Virtual CommPort Driver supplied with the Pump 11 Elite Series Pump Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by loo
5. Choose Stop to insert the Stop Command into the Method phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m 72 m E v2 lt gt A em gt Ip HARVARD APPARATUS Operating the Pump 11 Elite Series I O EVENT TRIGGERS 1 The Pump 11 Elite Series pump permits easy connection to external I O devices via a variety of 2 interfaces including a built in 15 pin D sub connector The Pump 11 Elite Series software incorporates simple commands for incorporating l O controls into your pump 3 Methods General trigger signals are integrated in the software The following procedure describes the use of 4 the Advanced Options I O commands Following the procedure is a description of the selection menu options for each of the I O command types Details for the electrical connection controls and pin out description for the 15 pin User I O Connector are provided in Appendix C of this manual The Pump 11 Elite Series manages trigger events through specific pins on the I O Connector port on the back of the pump The following details define pin logic e Trigger is communicated via Pin 4 6 e Event signal are communicated via Pin 2 I O OUT Trigger The I O OUT Trigger command is used to send a signal to an external device when the Step in the Method is reached HARVARD APPARATUS Select which trigger por
6. RAO a E e 63 Legacy rta MEG eue REM 64 Appendix KT E eee ee t qubd teme imr er M tU bEUR UM EU EU NM 65 Ws ese T 65 Upgrading Pump 11 Elite Series SorWware cccccecceeeeceeecteeeeeaeeeenenes 66 WOOO SSO OT uaseueseitesiu te udepatehensev aulae tdi iab map EU UM NE UE 67 Appendix E Ordering 1nrtormrmation eene 68 m Qn EE E v2 lt D A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Table of Contents General Information SAFETY INFORMATION Please read the following safety precautions to ensure proper use of your syringe pump If the equipment is used in a manner not specified the protection provided by the equipment may be impaired Io Prevent Hazard or Injury USE PROPER POWER SUPPLY The pump is supplied with an approved power supply and line cord To maintain the safety integrity of the device use only the following power supplies Cui Inc Globtek Inc Model 3A 242DB24 Model GT 41062 T3 Output 24V 1 0A Output 24V 0 75A Input 100 240V 50 60 Hz 0 8A Input 100 240V 50 60 Hz 0 6A USE PROPER LINE CORD Use only the specified line cord for this product and make sure line cord is certified for country of use The operating voltage range for the Pump 11 Elite Series is 12 30 VDC The universal power supply operating voltag
7. gt 20ml 50 Glass Plastic lt 250ul 30 Hamilton SGE lt 5ml 50 5ml 10096 The Pump 11 Elite can accommodate NOTE The Pico Plus Elite can accommodate syringes ranging from 0 5 pl to 60 ml single syringes ranging from 0 5 pl to 10 ml syringe and 0 5 ul to 10 ml dual syringe HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices Do APPENDIX B PUMP 11 ELITE MIN MAX FLOW RATES Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on syringe manufacturer Maximum Rate Minimum Rate Units Syringe Size Syringe ID 0 5 ul 0 103 mm pl min ul min 1 ul 0 146 mm l pl min pl min 2 pl 0 206 mm pl min l min 5 pl 0 343 mm i pl min ul min 10 pl 0 485 mm pl min l min 25 pl 0 729 mm pl min ul min 50 pl 1 030 mm pl min ul min 100 ul 1 457 mm pl min ul min 250 U 2 304 mm nl min ul min 500 ul 3 256 mm nl min ml min 1000 ul 4 608 mm nl min ml min 1 ml 5 151 mm nl min ml min 3 ml 8 585 mm nl min ml min 5 ml 11 99 mm nl min ml min 10 ml 14 43 mm nl min ml min 20 ml 19 05 mm nl min ml min 30 ml 21 59 mm nl min ml min 50 ml 26 59 mm nl min ml min m Qn EE EZ v2 lt gt A em gt KO 60 ml 26 59 mm nl min ml min HARVARD phone 508 893 8999 fax 508 429 5732 e mail bi
8. 60 ml 26 59 mm e Infuse Only D 3 T EIN i marly D o i O a E Uo e Withdraw Only Infuse Rate Select 75 ml min e Infuse Withdraw withdraw Rate Select e Withdraw Infuse 00 28 ml min e Custom Method Target Volume Select 60 ml The Method Selection Screen will be displayed The Method Selection HARVARD APPARATUS available Methods will be displayed based on the New Method model of syringe pump aust QuickStart create a new method Press the Method to select withdraw Only QuickStart Infuse Withdraw QuickStart Withdraw Infuse QuickStart IWANVA S d4Sf HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 T EIN i marly D o a a E uo IWOANVA S d4Sf HARVARD APPARATUS Configuring the Pump 11 Elite Series Syringe Selection 1 In the Quick Start Screen select the syringe button 2 Select the manufacturer type Then the available syringe sizes will be displayed In this example we have selected a Becton Dickinson Syringe 3 For a Custom Syringe use this selection if syringe manufacturer is not listed Custom Syringe Definition Display THE FORCE VALUE WHEN USING GLASS SYRINGES I CAUTION BE SURE TO LOWER 16 Quick Start HARVARD APPARATUS Method Select Quick Start Infuse Only x
9. Enter the settings Make sure to Syringe Select select the right syringe BD plastic 60 ml 26 59 mm Infuse Rate Select HARVARD APPARATUS Target Volume Time Select 60 ml Syringe Selection Custom Syringe Air Tite HSW Norm Ject Becton Dickinson Glass all types Select an existing syringe manufacturer or select Custom Syringe Cadence Science Inc Micro Mate Glass Hamilton Glass all types Harvard Apparatus Stainless Steel Syringe Size Selection Becton Dickinson Plasti pak DIS HARVARD APPARATUS Select the desired syringe size Syringe Selection Custom Syringe Air Tite Select an existing syringe HSW Norm Ject manufacturer or select Custom Becton Dickinson Syringe Glass all types P HARVARD APPARATUS Cadence Science Inc Micro Mate Glass Hamilton Glass all types Harvard Apparatus Stainless Steel Syringe Dimension Entry roe 1 HARVARD APPARATUS Enter the syringe settings Rc phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series a Flow Rate Selection Quick Start HABRVARD APPARATUS 1 In the Quick Start Screen enter the flow Bader npn eee rate s In this example we want to enter p Syringe Select 75 ml min BD plastic 60 ml 26 59 mm Infuse Rate Select 75 ml min Target Volume Select 60 ml 2
10. Publication 5420 002 Rev B Configuring the Pump 11 Elite Series 24 Setting Display Backlight Settings HARVARD APPARATUS The backlight can be adjusted G l Select the desired settings In the Settings screen select Backlight Audible Tuum pem Information Select the Backlight For example if you select ETT 80 the brightness of the display will be 80 of Force 100 E binio EA e Accept the changes made by pressing z Power U and go back to the Settings screen Running Ed the changes made by pressing and go back to the Settings screen For a Custom Value Set Backlight Level HARVARD APPARATUS Use this selection if the 96 is not listed Select an existing percentage or Press Set Other Value to enter a custom Enter the percentage Accept the changes made by pressing and go back to the Settings screen the changes made by pressing and go back to the Settings screen SetBacklightLevel HARVARD APPARATUS Backlight 100 Enter the backlight percentage D 3 T EIN i marly D o a a E uo TER o e EJ IVWANVA S d4Sf HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series 29 Setting the Pump Address Settings HARVARD APPARATUS Up to 100 pumps can be linked together Each pump Upgrade ETT
11. 47 Me TOO B rga o e Ru edm eo ERR 48 Exporting a Method to an External Computer eeesesssessss 49 Importing a Method from an External Computer cece ccceeeeeee neces 49 External PUMO SOO MM 50 Setting up Hyper Terminal tttm tnt tut e ctus tutu but ce ER Cue tbid 50 Puro cust lt vaT aper S gate crete scat tanec aside cuis i atendtscenu iun E EA 51 Using the Pump 11 Elite Series Pump Chain Commands ssc 51 Sr de 52 Song iae eye FR mS 52 G Set st LS ol 52 i System COMMAaNdS essemus ebat abate cendres ret tr dl e it sided wins 52 RUN 8 e eals NETTE E EET UELET 54 Z STR NANG T M 54 D Volpe CONAIS sena E E EEE E EEO E 55 M RE ereas e E E E E LU NAE 56 D Digital V O Commande ausus ustimrsrtrttut keia EAEEREN 57 in IF tenia aae TTT 57 pella orae NOS necs cpi RREN SAE Luv RREA 57 HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Table of Contents D SUBJECT PAGE NO ADDEBUICBS sirere NEEE UN BUUMIM dE DINEM T SUR EE CREATE QUE GM oM RET M AUSR 58 Appendix A Syringe Volume Diameter Reference Table ssusssse 58 Appendix B Min Max FOW r 59 Appendix C External Connections sssssssssse emnes 60 User VO Connector Specifications sucesos ausatiskete xps perd ierann neern ed 60 Julang VSB MEn e EIER 61
12. Block Lead Screw Yar mm Qu p X T B Prr Guide Rod Syringe Holder Bracket Clamp Knobs Run LED Half Nut Button User Interface Screen with Protective Antiglare and D EMI Shield p 00 00 00 oa Pump Il Elite Universal Power Input RJ 11 Option Main Switch 1 0 Connector Power Source Foot Switch When powering a 1 pumpin a chain us through an RS 485 l us Q cable toggle I switch to RS 485 OO Only one pump can USB Serial Input 9S9 bepowered from Type B 1 OCINE master through RS 485 Connection E RS 485 Pump to Pump I Programmable pumps only RS 485 D I L I POWER CONNECTIONS 1 Plug the power cord in The Pump 11 Elite Series has a universal power input connector on the rear of the unit The operating range for the Pump 11 Elite Series series is 12 30 Vdc 2 Turn on the main power switch CAUTION DO NOT CONNECT TO FIREWIRE PORTS ON A COMPUTER DAMAGE MAY OCCUR TO THE PUMP OR COMPUTER Best performance guaranteed when using 24V power supply phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com web www harva rdappa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 T EIN i marly D o i a E uo IWANVA S d4Sfn SYRINGE LOADING 9 The Pump 11 Elite Series can accommodate up to two syringes Dependin
13. Depending on the Method the appropriate set rate screen will be displayed To change the units press the units Set Infuse Rate HARVARD APPARATUS Enter the rate s and units D 3 5 u marly D o a a E uo 3 In the Units Selection Screen select the volume Units Selection l HARVARD APPARATUS and the time base Current selection Select the unit IWOANVA S d4Sf HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series 18 Target Volume Time Selection Quick Start HARVARD APPARATUS S Method Select 1 In the Quick Start Display enter the target Duck bat Infued Only Enter the settings Make sure to Syringe Select select the right syringe volume or time In this example we want a volume of 60 ml Infuse Rate Select 75 ml min Target time is only allowed in infuse only or withdraw only Methods NOI Target Volume Time Select 60 ml 2 Depending on the Method either Target Volume I W or W I will appear or Target Volume or Target Time Selection I only or W only For time enter h m s or press again to select secs Time is accurate to 0 01secs Remember to enter the NOT 3 To change the units press the units Set Target Volume or Time HARVARD APPARATUS Enter the target volume or time
14. Method while the pump is running 6 Choose Export Method to Pump 7 The pump will display a confirmation message in the Message Area on the screen when the export is complete Note that long Methods may take a few seconds to export t Do not change screens while Importing Exporting a Method 8 Before disconnecting the pumps verify that the Method was successfully exported by choosing Method Select from the Method Main screen on the destination pump then browse the available Methods to verify that the exported Method is shown in the menu For additional details on the physical interfaces used to connect the Pump 11 Elite Series to external devices refer to the External I O Connections section in the Appendices HARVARD APPARATUS Method option screen m Qn EE EX Qn lt D A em gt EA HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m 72 EE EX v2 lt D A m gt TTS HARVARD APPARATUS EXPORTING A METHOD TO AN EXTERNAL COMPUTER Follow these instructions to export a Method to an external computer 1 Connect the pump to the external computer via USB cable 2 On the computer open the HyperTerminal application and verify that the computer can communicate with the pump 3 Atthe pump from the Method Main s
15. bracket Tighten the mechanical stop collars 9 using allen wrench 12 to prevent excess travel of the pusher block The syringe barrel clamp 10 may need to be flipped depending on your syringe size To do so completely unscrew and remove the syringe barrel clamp knob from the syringe block Then remove lock nut 11 Flip the bracket and reassemble CAUTION BE SURE MECHANICAL STOP COLLARS ARE POSITIONED PROPERE Opie EN PLUNGER FROM BOTTOMING OUT UN GLASS SYRINGES HARVARD phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series 15 Configuring the Pump 11 Elite Series A Method is easy to set up The Pump 11 Elite Series is easily configured When the unit is powered up the Quick Start Quick Start HARVARD APPARATUS Screen will be displayed Method Select Quick Start Infuse Withdraw Enter the settings Make surg to Syringe Select set a target volume BD plastic 60 ml 26 59 mm Infuse Rate Select 75 ml min Withdraw Rate Select 88 28 ml min Shall a Target Volume Select 60 ml Method Selection Quick Start HARVARD APPARATUS Method Select In the Quick Start Screen select Method Quick Start Infuse Withdraw A Enter the settings Make sure to available Methods depend on the pump model EE BD plastic
16. down if needed From Step 1 3 times 36 DELETING A STEP FROM A METHOD 1 From the Method Main screen choose the Step Definition button to enter the Method Steps Summary screen 2 Highlight select with a single touch the Step that you want to delete 3 Choose the Delete Step button The application will display a warning message in the Message Area asking you to confirm your deletion request 4 To confirm the deletion choose the Delete Step button again Defining Method Step Parameters This section of the User Guide describes each of the functions available for use in Pump 11 Elite Series Existing and User Defined Methods The information in this section is designed to help you set the individual parameter values in each of the Method Step options and to properly use the Advanced Options commands The information that follows is organized into two sections Pump Operation Profiles e Advanced Options Commands and lO phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 37 PUMP OPERATION PROFILES The Pump 11 Elite Series Existing Methods and all User Defined Methods use Pump Operation Profiles as part of the Methods controlling the pump These profiles are combined with a syringe selection and any Commands or Input Output trig
17. is interrupted during operation an alarm will sound if alarm is activated to alert the user to the interruption When power is restored an onscreen message is displayed to indicate the interruption Quick Start Infuse Only BD plastic 60 ml 26 59 mm 75 ml min 5 Overload Protection The Pump 11 Elite incorporates resettable fuses for overload protection In the event of an overload power to the pump may be disconnected Try to determine and remove the source of the overload wait a few minutes The Pump 11 Elite Series Pump will notify you of a power failure and then re apply power to the pump with a message when power is restored m Qn EE E v2 lt D A am gt AQ HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 09 APPENDIX E ORDERING INFORMATION Standard Version Description Single Syringe Dual Syringe Pump 11 Elite Infusion Only 70 4500 70 4501 Pump 11 Elite Infusion Withdrawal Programmable 70 4504 70 4505 Pump 11 Pico Plus Elite Infusion Withdrawal Programmable 70 4506 Options Description Catalog Number RS 232 RJ 11 Connectors Option 70 3030 Only available with Programmable Models If needed must be ordered at the same time as the Elite pump Accessories Description
18. or editing a Ramp Rate profile you will define three primary operating parameters The Flow Method defining the direction of flow infuse or withdrawl e The Flow Rate at the beginning and end of the pumping cycle The Target Time that determines the total length of time over which the ramp will be calculated During a Ramp Rate profile the Pump 11 Elite Series varies the flow rate linearly between the starting rate and ending rate over the full time period entered A Ramp Rate profile can be applied to an increasing or decreasing rate application When creating or editing a Ramp Rate profile you can set the rate in ml ul nl or pl units of volume using sec or h m s hours minutes seconds as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse and Withdraw Rate screens TO SET UP EDIT A RAMP RATE PROFILE E 72 EE EX n lt gt A em gt 1 From the Step Definition screen for a Ramp Rate profile choose the Set Mode button to toggle the Flow Method between Infuse and Withdraw operation HARVARD APPARATUS Infuse Enter the step settings Start 0 ml min End 0 ml min D second s Rc Step Definition screen for a Ramp Rate profile HARVARD APPARATUS web WWW harvardapparatus com 2 10 11 12 Operating
19. return to the Method Selection screen phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 48 PUMP 11 ELITE SERIES METHOD TRANSFER A METHOD BETWEEN PUMPS EXPORT AND IMPORT Follow these instructions to transfer a Method between two Pump 11 Elite Series pumps The Pump 11 Elite Series pump permits the daisy chaining 1 Connect two pumps together via a RS 485 of multiple pumps as well as integration with control by firewire type cable and set the address on the an external computer through HyperTerminal To facilitiate first pump to 00 using the Set Pump Address operation in these Methods the Pump 11 Elite Series command in the Settings menu application software includes a variety of commands designed to simplify the export import of Methods between the pump and external devices This section outlines the use of these commands 2 Set the address on the second pump to something other than 00 using the Set Pump Address command in the Settings menu 3 From the Method Main screen choose Method Select then choose the Method you would like Import will only work when the destination to export pump has 0 or 1 Method stored 4 Choose the File Options button 5 Choose the Pump button and enter the address for the pump you would like to receive the Do not attempt to Export Import Methods
20. save your changes and return to the Step Definition screen phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B MANAGING PUMP 11 ELITE SERIES METHODS All Existing and User Defined Methods are stored in the Method List for ongoing use Over time you may want to manage previously created Methods to keep your Method List easy to navigate In addition the Pump 11 Elite Series allows you to export Method information to an externally connected pump or to a computer that will be used to control operation This section of the Guide Pump 11 Elite Series Quick Start Methods Infuse only Withdraw only I W or W I Saving A Copy Of A Method Follow these instructions to save a copy of a Method under a new name 1 From Quick Start screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want to save a copy of then choose the File Options button to 3 Choose Save As Copy to access the Method will be displayed in the text entry box 4 On the Method Name screen type the new name for your Method using the onscreen keyboard Choose the Symbols Numbers numbers and symbols that can be included in the Method Name To switch the keyboard back to letters Method choose the Letters button ABC 15 cha
21. while ramping Clears both the infused and withdrawn volumes Quick Quick Start mode only Start mode only Command format Command format wramp start rate end rate rate units ramp cvolume time in seconds CWVOLUME ind response Clears the withdrawn volume Quick Start mode only It gt R t set up lt cr lt If gt Ramp not set up lt cr gt Command format If B prompt cwvolume or IVOLUME SIL Hae Staring tales er eT Displays the infused volume Quick Start mode only lt lf gt Ending rate xl xxx cr CGonmand fort lt lf gt Ramp time seconds lt cr gt ivolume lt lf gt lt prompt gt Query response WRATE If Bi xl lt cr gt Sets or displays the withdraw rate Quick Start mode lt lf gt lt prompt gt only Command format SVOLUME TERI MEET Sets or displays syringe volume Quick Start mode only Command format Query response lt f gt xl xxx cr NOUM lt lf gt lt prompt gt TVOLUME Sets or displays the target volume Quick Start mode only Volume commands Command format CIVOLUME tvolume target volume volume units Clears the infused volume Quick Start mode only Query response Command format lt lf gt Target volume not set lt cr gt civolume lt lf gt lt prompt gt CTVOLUME or Clears the target volume Quick Start mode only elf gt xlboocecr Command format lt lf gt lt prompt gt ctvolume WVOLUME
22. 3 8999 fax 508 429 5732 e mail bioscience pharvardapparatus com APPARATUS web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 67 Upgrading Pump 11 Elite Series Software First time users will need to install the Pump 11 Elite Series USB driver as well as the bootloader driver file Pump 11 Elite Series Bootloader inf Reference USB 2 Connect the pump to PC using a USB cable Virtual Compact Driver Installation 1 Upload the latest software version to your desktop format is filename dfu 3 On the Settings screen press the Upgrade Software button twice to enter Boot Loader Mode The pumps screen will be blank and the light should be flashing im Pump Flash Upgrade Application Select Pump To Upgrade To exit boot loader mode without upgrading Eie Rump ih elf s eli N _ the software power cycle the pump V erifu after download Optimize Upgrade duration Remove some FFs 4 Disconnect then reconnect the USB cable Upgrade Verify 5 From the CD provided with the pump open the Pump 11 Elite Series Flash Upgrade File correctlyloaded Application 6 Check Verify after download then click Aes browse Browse to the file previously saved on your desktop and click open 7 Click upgrade The previous software will first be erased the new software will be loaded and finally the software will be verified 8 Dis
23. 5 7 iLe ILo EINEN REE EID Li 21 phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 5 u marly D o i O a E Uo IWANVA ES ENIS HARVARD APPARATUS Configuring the Pump 11 Elite Series Locking the Touch Screen A touch screen lock function can be enabled to prevent accidental operation or interruption of operation Unlocking the touch screen requires a password defined by the user Setting the Password To set the Password go to the Settings screen Press the Set Password button Enter your desired password up to 8 digits and press the ACCEPT button Turning the Touch Screen Lock On Press the Lock button twice to lock the touch screen Turning the Touch Screen Lock Off Press the LOCK button to bring up the Enter the Pump Password screen Enter your password and press the ACCEPT button NOI If you forget your password contact Harvard Apparatus Technical Support at 508 893 8999 HARVARD APPARATUS Upgrade Change Logo Sa Select the desired settings Audible Pump Alarms Information Baud Rate Force 100 115200 Backlight 100 Address 0 G re E Power Up Set Password a Running Ed Quick Start HARVARD APPARATUS Method Select Quick Start Infuse Withdraw Enter the settings Make sure to Syringe Select set a tar
24. 93 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m Qn m Er v2 lt D A m gt TTS HARVARD APPARATUS Operating the Pump 11 Elite Series I O IN Event The I O IN Event command is used to have the pump pause and wait for a signal from an external device or for a manual user input at the touch screen These external signals are communicated via Pin 2 on the 15 pin I O Connector port HARVARD APPARATUS Select which type of event the step will wait for Rc The following options are available when setting an I O IN Event command e Input High to Low Sets the pump to wait for a logic low input signal falling edge e Input Low to High Sets the pump to wait for a logic high input signal rising edge e User Press Sets the pump to wait for manual input from the user at the touch screen screen touch 1 46 TO INSERT AN 1 0 IN EVENT COMMAND From the Method Main screen choose Step Definition to enter the Step Definition screen Highlight select once the Method Step that you want the lO IN Event to follow Choose the ADD STEP button to access the Method Steps Menu screen LJ Choose the I O IN Event button to enter the Event Step Definition Screen Select either Input High Input Low or User Press as appropriate Choose the Accept button to
25. ARATUS Messages There are different types of messages Error messages are shown in yellow e Critical e Run mode e Method e Settings Notices alarms confirming and verified messages are show in green e Alarms e Confirm e Verified Entering Values Entering new values is easy Use the keypad to enter the number Backspace key Decimal point Cancel and go back to the previous screen Accept the value and go back to the previous screen Quick Start HARVARD APPARATUS Method Select Quick Start Infuse Only POWER FAILURE Syringe Select BD plastic 60 ml 26 59 mm PRESS TO CLEAR ERROR Infuse Rate Select 75 ml min Target Volume Time Select 60 ml Method Main HARVARD APPARATUS Method Select HMM Syringe Select BD plastic 60 ml 26 59 mm Step Definition Step 1 Constant Rate Step 2 Autofill Step 3 Ramp Step 4 Repeat Syringe Dimension Entry Diameter rnm 26 59 Enter the syringe settings CNN 7 ILs JLo EIE EEE L Lo ie HARVARD APPARATUS Lid DE phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com HARVARD APPARATUS Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m 72 m Er Qo lt gt A Gi gt E HARVARD APPARATUS Product Overview NE Retaining Brackets Syringe Barrel Clamp 1 OPERATING INSTRUCTIONS Pusher
26. ARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B General Information WARRANTY AND REPAIR INFORMATION CAUTION REFER TO SAFETY INFORMATION AND SETTING UP THE HARVARD APPARATUS PUMP 11 ELITE SERIES BEFORE PLUGGING IN THE PUMP Manual Description This manual is designed to provide all operational and Method information required to operate and maintain the Pump 11 Elite Syringe Pumps The functions and features are described in the Technical Specifications section Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original consumer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitat
27. Catalog Number RS 485 Cable for Pump to Pump Communication 0 5 m 2 ft 70 4000 RS 485 Cable for Pump to Pump Communication 1 m 3 ft 70 4021 RS 485 Cable for Pump to Pump Communication 2 m 7 ft 70 4001 RS 485 Cable for Pump to Pump Communication 9 m 30 ft 70 4020 E USB Cable for PC to Pump Communication 2 m 7 ft 70 4002 da USB Cable for PC to Pump Communication 5 m 16 ft 70 4003 T Cable Assy Daisy chain Legacy RS 232 RJ 11 0 5 m 2 ft 55 7760 J Cable Assy Daisy chain Legacy RS 232 RJ 11 2 m 7 ft 72 2478 Y Line Cord US 115 v 5113 001 n Adapter Digital I O 70 4005 gt Adapter D sub 15 to Term Blk 70 4006 a Footswitch w Phono Plug 70 2215 D Adapter for 25ml 50mI Hamilton GasTight syringes 55 8000 A Hex Key 3 32 5012 005 Lubricant SuperLube 1cc 70 4013 For additional items such as Syringes Tubing Syringe Heaters Spill Sensors Temperature Controllers Nano Fluidic Accessories and much more please visit our website www harvardapparatus com or call 800 272 2775 HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Declaration of Conformity Manufacturer Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 U S A Phone 508 893 8999 We herewith declare that the following product Product Name Pump 11 Elite S
28. D 3 5 u marly D o a a E uo 4 In the Units Selection Screen select the Units Selection HARVARD APPARATUS volume units Current selection IVWANVA S d4Sf HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 T EIN i marly D o a a E uo IVWANVA S d4Sf HARVARD APPARATUS Configuring the Pump 11 Elite Series Previewing the Quick Start It is simple to view the Pump Quick Start on the RUN display Press to view Pump Quick Start L After you have verified the configuration Press D to run the displayed configuration ig Quick Start HARVARD APPARATUS Enter the settings Make sure to set a target volume Method Select Quick Start Infuse Withdraw Infuse Rate Select 75 ml min Withdraw Rate Select 88 28 ml min Target Volume Select 60 ml Press to preview configuration Run HARVARD APPARATUS Infuse Only BD plastic 26 59 mm 60 00 ml Target 60 ml Ready to infuse Infuse 75 ml min Flow 3 Direction Time Elapsed 00 00 00 Time Remaining 00 00 48 Total Inf Volume O ml Force Level 100 Configuring the Pump s General Parameters The pump has basic parameters that should be configured during initial start up Once these parameters are entered they probably won t be changed
29. D phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 42 Autofill Cycling Profile HARVARD APPARATUS The Autofill Cycling Profile is an automated 04 28 09 1 38 44 PM Infuse Withdraw or Withdraw Infuse step that operates Enter your settings to the left and then press the Enter button at constant but separately defined flow rates for the INF 0 ml min WD 0 ml min green check mark to accept or TTE press the Cancel button red X withdraw and infuse operations The Autofill profile is toretum to te area Sacer designed to work in conjunction with an autofill valve 50 ml per Cycle that controls the switching of flows from a reservoir for refilling the syringe to the target infusion subject 0 ml Infuse Cycles Flow Rate L e an ian ea i ni De i Rn n in n ar Total Volume Withdraw Time Volume When creating or editing an Autofill Cycling profile you will define the following operating parameters The Flow Mode defining the cycle Infuse Withdraw INF WD or Withdraw Infuse WD INF The Flow Rates to be used during Infuse Withdraw operations e The infusion volume to be delivered during each pump cycle e The total volume to be delivered during all cycles or the total number of Infuse Withdraw or Withdraw Infuse cycles that you want t
30. Displays the withdrawn volume Quick Start mode only m Qn m E v2 lt gt A em gt KO For commands requiring rate units use the Command format N _ following format m u n p h m s ex m m milliliter minutes ex n s nanoliter second wvolume Query response lt If gt xl lt cr gt lt lf gt l lt prompt gt HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m Qn m Er v2 lt D A m gt TTS HARVARD APPARATUS Operating the Pump 11 Elite Series Time commands CITIME Clears the infused time Quick Start mode only Command format citime CTIME Clears both the infused and withdrawn times Quick Start mode only Command format ctime CTTIME Clears the target time Quick Start mode only Command format cttime CWTIME Clears the withdrawn time Quick Start mode only Command format cwtime ITIME Displays the infused time Quick Start mode only Command format itime Query response Ift HHE HE seconds cr If B prompt Or If HE JHH HH HH CER If prompt 56 TTIME Sets or displays the target time Quick Start mode only Command format ttime target timej Query response lt lf gt Target time not set lt cr gt lt lf gt lt prompt gt or If HE HE seconds lt
31. HyperTerminal Main Menu screen In HyperTerminal choose Transfer Send Text File Select the Method that you want to import to the pump and click Open Before disconnecting verify that the Method was transferred by choosing Method Select from the Method Main screen at the destination pump then browse the Method menu to confirm that the new Method is now saved at the pump phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B EXTERNAL PUMP CONTROL This section of the Pump 11 Elite Series manual describes the control of the pump using an external computer device If communicating via the USB Port see Appendix C for one time installation instructions of USB Driver Setting Up HyperTerminal HyperTerminal is a Windows application designed to support the external control of devices such as the Pump 11 Elite Series pump through a USB connection The following instructions describe the configuration of the HyperTerminal application 1 Select Start All Methods Accessories Communications HyperTerminal 2 Enter a name for a New Connection i e Pump 11 Elite then click OK Connection Description ab New Connection Enter a name and choose an icon for the connection Name Pump 11 Elite Icon 3 Select the Virtual Comm Port from the Connect u
32. In using the Pump Chain commands you will need to assign each pump in the pump chain a unique address using the Set Pump Address command in the Settings menu The address range is from 00 to 99 This address value is used to identify which pump is to receive a command and which pump is responding The first pump in the chain the one connected to the computer must use address 0 zero Configure each pump with its assigned address and baud rate as described in the Pump Settings section of this guide oT If the pump address is nonzero the one or two digit pump address precedes the command For example to set the infuse rate for pump 12 the command would look like 12irat 3 2 u m If the USB port is used on a pump with a nonzero address the pump address does not need to be used Every line of the response from a pump with a nonzero address is prefixed by the two digit pump address prefixed with a O if necessary followed by a colon No colon is used to seperate the pump address from the prompt however In the command list below the following convention is used U Required parameter Optional parameter Separator between parameter NOTE When daisy chaining using the choices optional RJ 11 connectors all pumps must use the same baud rate Values above 38400 Numeric value without preceding are recommended eer HHH Numeric value with preceding zeros NOTE If you are using t
33. Press Power U es Set Password B ately Ed Puma Information HARVARD APPARATUS Model Pump 11 Type Programmable Software Version MARES Reset the Maintenance Due as needed Maintenance Due 93 hours Pump Address 0 Maintenance Reset D 3 5 u marly D o a AL E Uo Confi gu rin ga New Custom Method Method Main HARVARD APPARATUS To display the Method Selection screen press Method ienn syringe and Syringe Select BD plastic 60 ml 26 59 mm Step Definition Step 1 Constant Rate Step 2 Autofill Step 3 Ramp Step 4 Repeat The Method Selection screen will be displayed Method Selection HARVARD APPARATUS IVWANVA S d4Sf Infuse Only Select an existing method or View Methods up a page QuickStart create a new method withdraw Only QuickStart QuickStart View the Methods down a page n r1 Views up the Method n Views down the Method M HARVARD phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series ou Configuring Custom Methods 15 CHAR MAX The first thing to do on a custom Method is to name the Vei ej re s m v U OVP iini T s I SILT EI EJESGIMIIEILILC Go back to the Method Selection screen Ed D Select the Syringe manufacturer and Size HARVARD APPARATUS To define the steps for the custom Method press St
34. Programmable E E Software Version BBH Reset the Maintenance Due as Confirm the Maintenance Reset by pressing the needed Maintenance Reset again Maintenance Due 93 hours Pump Address 0 Accept the changes made by pressing and go back to the Settings screen Reset the changes made by pressing and go back to the Settings screen E Ed IVWANVA S d4Sf WE Free RTOS v6 0 1 www freertos com HARVARD phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series 27 Tilt HARVARD APPARATUS The touch screen can be tilted for ease of use when the Upgrade Software Change Logo pump is placed on its side Select the desired settings Pum Information Baud Rate Audible Alarms 0 Force 100 115200 Address 0 TILT cc Power Up Running In the Settings screen Press to rotate the touch screen Backlight 100 counter clockwise Set Password Press to rotate the touch screen clockwise Press E to return the touch screen to its 2 i Quick Start Infusion Only original orientation Sherwood 38 4 mm 140 ml R Le IS 220 975 ml min A e HARVARD APPARATUS Enter your settings to the left and press the RUM or Run Preview button n m Qn m Er v2 lt D A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscien
35. ai Pump 11 Elite amp Pump 11 Pico Plus Elite USER S MANUAL rvardapparatus com Harvard Apparatus Pump 11 Elite amp Pump 11 Pico Plus Elite Series Publication 5420 002 Rev B HARVARD os APPARATUS emai bioscience harvardapparatus com Table of Contents Table of Contents SUBJECT PAGE NO General Information sss sese cesses eee eto Uam eee 6 eal et Iai sepse e sarrerei titre pn E ea r a eE iai 6 Warranty and Repair letest e 7 Ete ent ee 7 UO sn ATT 7 Repair FACIES ana H n 7 Serial Numbers 7 GADEA TE EE E E 7 DEE EES N N A AE A E E E A ETE 8 Product Overview css s esse eee e eee 9 xu odo 10 Pump 11 Elite Series Touch Screen Button Reference 11 Selecting an Item from a Menu US 12 PS OS R n 12 sai od TT 12 Operating InstF ctlOfiSseseretixt tutte metae bine na et o FH t epbb E Dub tst SH RU eta DOR bent le Ca o we Rent ee MEET M 13 i Se mme o aio NER RENTE 14 da Configuring the Pump 11 Elite Series sss esse ee ees eee eee enen 15 a Method SECU 9 8 MR IRE RM 15 gt SV TINGS e Le 16 FOW ene a T 17 gt Target Volume Time Selection sssssssssse mm enn 18 ib Previewing the Quick SUAE Lasautedusensanmasutubtcntveqidein guests qoM Med Eie 19 Configuring the Pump s General Parameters ssssssseeH ee 19 Setting the Audible Alarms ssssssesee e Imm mmn nnns 20 For Near Ena of R n se
36. and installation options ey Search for the best driver in these locations Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy CD ROM IV Include this location in the search c USER BHE Projects HarvApp SyringePumps P Browse C Don t search will choose the driver to install Choose this option to select the device driver from a list Windows does not guarantee that the driver you choose will be the best match for your hardware 4 lf a warning message regarding Windows Logo testing is displayed choose Continue Anyway to proceed with the installation Hardware Installation The software vou are installing for this hardware Syringe Pump P Vista has not passed windows Logo testing to verify its compatibility with Windows amp P Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the Future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway eo id RT Wil a 5 The Virtual Commport software will be _ installed When the software installation is Completing the Found New complete click Finish to close the wizard Har
37. and select Echo typed characters locally then click OK to complete the setup ASCII Setup ajx ASCH Sending Send line ends wih ine feeds Maseskesuusuususkueuusu eu suse u eu uua s ssa ses sisse nura M Echo typed characters locally Ke een nns Line delay 10 Character delay o milliseconds ASCII Receiving Append line feeds to incoming line ends Force incoming data to 7 bit ASCII v wrap lines that exceed terminal width NOTE To determine if your pump is connected correctly in Hyperterminal s main window press Enter a should appear If not connected properly close Hyperterminal turn off the pump unplug then re plug the USB cable turn on the pump and re open Hyperterminal HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience gharvardapparatus com APPARATUS web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B E 72 EE EX n lt D A m gt AQ Operating the Pump 11 Elite Series PUMP CHAIN COMMANDS The Pump Chain commands allow all pump control information to be managed from an external computer source These commands can also be used to control a series of pumps up to 100 from a single computer interface Pump 11 Elite Series commands are communicated to the pump via a USB port interface through a terminal Method such as Harvard Apparatus Method Manager or HyperTerminal
38. are identified with a 15 character alphanumeric name for easy identification and can be stored on the computer and downloaded at future dates Extensive programming options allow the user to duplicate configurations append one configuration to another rename Methods delete Methods export Methods to a PC or export a Method to another pump This feature can ensure consistency and accuracy of multiple pumps Preprogrammed profiles are selectable from Constant Rate Ramp Autofill and Gradient These standard profiles make configuration easy to set up If more complexity is needed the user can select from advanced Method programming options including Time delays e Repeating steps e Stopping the pump e Triggering the pumps TTL output e Accepting an event input in such as a user touch or motor stall Events can trigger the pump to withdraw or infuse Product Overview 10 The pump Information settings screen shows the pumps software version maintenance interval and pump address The pump s alarm configurability includes alarms for near end of run user selectable completion of run power up keypad clicks and stall detection Pump Models Infusion Only single and dual syringe models These two models support infuse only Quick Start Methods with user defined flow rates and selectable target volume or target time values to control the total infusion time Infusion Withdrawal Programmable single and dual syrin
39. ates are selectable with user selected engineering parameters on an easy to view run screen The touch units from ml ul nl pl and hours minutes and screen lock feature prevents accidental configuration seconds changes ensuring the Methods integrity is retained l l l l Up to 16 kg 35 Ibs of linear force is available This force A green led light on the front panel makes it easy to see is user adjustable to ensure the right force is applied for if the pump is running Once the pump starts to run the the various syringe sizes display shows the pumps is running and the LED turns on This will make it easy to see the pumps status Te HEE p TGSREMBIUSS LUDUM ingressing into the sealed display or electrical The pumps are versatile and can be interconnected connections through the RS 485 interface Pump 11 Elite Series models can be mixed and matched in the daisy chain offering maximum flexibility Up to 99 pumps can be linked together through the RS 485 interface This interface is easy to use provided each pump is assigned its own unique pump address For limited laboratory space the Pump 11 Elite Series can be placed on its side to reduce the footprint by 5596 The display can also be tilted to allow the user to operate the pump vertically E 72 m ue n lt gt A m gt Ip Notifications and error messages are displayed for the user to acknowledge eliminating any guesswork about problems Flow performance is opti
40. can be given a unique address Select the desired settings In the Settings Screen press Pump Address fuam SUES ES Valid entries are 00 to 99 aT Enter t pump andress Backlight 100 TILT fror CC E Accept the changes made by pressing and go back to the Settings screen odds qaia Ed the changes made by pressing and go back to the Settings screen Set Pump Address HARVARD APPAR ATUS Enter a value between 0 and 99 Pump Address o Rc Setting the Baud Rate Settings HARVARD APPARATUS The Pump 11 Elite Series have an RJ 11 option for serial communications Select the desired settings To adjust the baud rate from 9600 to 115 200 in the fame a Settings screen press Baud Rate Accept the changes made by pressing and go back to the Settings screen Running the changes made by pressing and go back to the Settings screen D 3 5 u marly D o a a E uo Set Baud Rate HARVARD APPARATUS LI 9600 Select a baud rate e 19200 IVWANVA S d4Sf e 38400 e 57600 HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series 26 Power Up Running ings HARVARD APPARATUS If Power Up Running is selected the pump will power Upgrade Change Logo Software itis dodod satt up running when power resumes a nN pes Audible Pump Alarm
41. ce Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 T EIN i marly D o i O a E Uo IWANVA S d4Sf HARVARD APPARATUS Operating the Pump 11 Elite Series Operating the Pump The Pump 11 Elite Series is easy to operate because all the pump parameters are shown on the RUN Screen Depending on the Method of the pump different parameters will be shown on the display The Pump 11 Elite Series Run Screen is shown below Infuse Only BD plastic 26 59 mm 60 00 ml Target 60 ml Infuse 75 ml min Flow Direction Time Elapsed 00 00 00 Time Remaining 00 00 48 Total Inf Volume O ml Force Level 100 28 HARVARD APPARATUS Ready to infuse olla i ias gt Running the Pump Simply press the gt button to start the pump When the pump is running the button Is shown Press the m button to pause the Method Press the g resume running the pump Press the 9 to clear the counters and timers The fast forward and fast reverse key are not active when the pump Is running Run HARVARD APPARATUS Infuse Only BD plastic 26 59 mm 60 00 ml Target 60 ml Ready to infuse Infuse 75 ml min Flow Direction ECT Taw Time Elapsed 00 00 00 Time Remaining 00 00 48 Total Inf Volume O ml Force Level Run HARVARD APPARATUS Infuse Withdraw BD pla
42. connect the USB cable and power cycle the pump m 72 EE E v2 lt D A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 68 TROUBLESHOOTING 1 Infusion Accuracy To ensure infusion accuracy always use new syringe s and measure syringe bore diameter and enter actual dimensions in millimeters mm using the Custom Syringe entry option Additionally make sure that the guide rods and lead screw are properly lubricated v Itis recommended that you save your N Methods periodically by uploading them to a PC Pump 11 Elite Series Display Error Messages 2 Motor Stalled This indicates that the pusher block travel has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing occlusion syringe plunger binding incorrect positioning of mechanical stop collars or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and or increase the force setting then press RUN The pump will continue the interrupted procedure where it stopped 3 Out of Range A value was entered or encountered in a pump Method that was beyond the pumps limits 4 Power Failure Notification HARVARD APPARATUS If power to the pump
43. cr gt lt lf gt lt prompt gt or If 2 AH HH HER CE lt lf gt lt prompt gt WTIME Displays the withdrawn time Quick Start mode only Command format wtime Query response lt if gt seconds lt cr gt lt lf gt lt prompt gt Or If HE IHH HH H lt CE lt lf gt lt prompt gt Digital I O commands INPUT Reads and displays the trigger input port status Command format input Query response lt lf gt The trigger input is low lt cr gt lt lf gt lt prompt gt or lt lf gt The trigger input is high lt cr gt lt lf gt lt prompt gt phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series D OUTPUT sets the level on the output port The Pump 11 Elite does not contain limit _ switches so will be displayed Command format output 1 high low Flag three is the stall status and will be S if the pump has stalled Otherwise it will be Internal commands Flag four is the trigger input state and will be T if high and if low STATUS ae l Displays the raw status for use with a controlling rad no ul Etudier i toe infuse and W for withdraw computer Flag six is the target reached status It will be T if the Command format l o target time or volume was
44. creen choose Method Select then choose the Method you would like to export 4 Choose the FILE OPTION button 5 In HyperTerminal choose Transfer Capture Text 6 Enter the File Name that you want to save the Method under then choose Start 7 Choose Export Method to PC 8 The pump will display a confirmation message in the Message Area on the screen when the export is complete Note that long Methods may take a few seconds to export 9 Atthe destination computer in HyperTerminal when the Method is done transferring the message mend will appear onscreen 10 In HyperTerminal choose Transfer Capture Text Stop Your Method is now saved on the external computer Operating the Pump 11 Elite Series 49 IMPORTING A METHOD FROM AN EXTERNAL COMPUTER Follow these instructions to import a Method from an external computer 1 Connect the pump to the external computer via a USB cable and set the Address value to 00 using the Set Pump Address command in the Settings menu On the computer open the HyperTerminal application and verify that the computer can communicate with the pump In HyperTerminal click on the Properties icons and choose the Settings tab on the pop up box that is displayed Click on the ASCII Setup button In the Line Delay field enter 500 This will set a 500 millisecond delay after each line is transferred during the import process Click OK twice to return to the
45. d the versatility of the system by permitting convenient looping chaining of events sending signals to external devices or accepting inbound signals from external devices These Advanced Options controls are described in this section of the Guide phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 T EIN i marly D o i a c Uo IWANVA S d4Sfn HARVARD APPARATUS Operating the Pump 11 Elite Series Delay The Delay Command is used to Insert a time delay ina procedure You can set a time delay between 0 2 seconds up to 99 99 99 99 hours 99 minutes 99 seconds TO INSERT A TIME DELAY IN A METHOD 1 From the Method Main screen choose Step Definition to enter the Step Definition screen 2 Highlight select once the Method Step that you want the time delay to follow 3 Choose the Insert Step button to access the Method Steps Menu screen 4 Choose Delay to enter the Set Delay Time screen Set Delay Time secs HARVARD APPARATUS Enter the time delay LI Advanced Options Set Delay Time screen 5 Enter the desired Time using the keypad 6 If the Units of Time associated with Time are not correct for your application select the Units button to toggle between sec seconds 0 2 seconds minimum allowable value and h m s hours minute
46. dware Wizard The wizard has finished installing the software for E Syringe Pump XP Vista m 72 m E V2 lt gt A Gi gt KO Click Finish to close the wizard HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 64 RS 485 CAUTION DO NOT CONNECT TO FIREWIRE PORTS ON A PC DAMAGE MAY OCCUR TO PUMP AND OR PC Mating Face RS 485 In Signal RS 485 Out Signal Notes PWR IN PWR OUT 30V Protected by GND GND resettable fuse RS 485 A TIED TO PIN 4 7100 QO w to GND termination RS 485 B TIED TO PIN 3 TIED TO PIN 6 RS 485 A TIED TO PIN 5 RS 485 B RS 485 In Out IEEE 1394 Sockets PUMP RS 485 RS 485 PUMP RS 485 RS 485 PUMP ADDR OQ OUT IEEE 1394 IN ADDR 1 OUT EEE o IN ADDR n Cable Cable Daisy Chaining via RS 485 MASTER PUMP RS 485 RS 485 PUMP RS 485 ADDR OUT IEEE 1394 IN ADDRX OUT Cable Gradient Connections via RS 485 When daisy chaining with RS 485 at Power may be removed from any group of minimum every fourth pump must be self pumps except the first group without powered i e another stand alone unit affecting communications down stream This can be affected by Force Selection a Qn EE E n lt gt A em gt When applying power to pumps in a dats
47. e Netherlands TL 31 0 594 769026 www instechlabs com Pumps syringe
48. e range is 100 240 VAC 50 60 Hz GROUND THE PRODUCT This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded MAKE PROPER CONNECTIONS Make sure all connections are made properly and securely Any signal wire connections to the unit must be no longer than 3 meters OBSERVE ALL TERMINAL RATINGS Review the operating manual to learn the ratings on all connections AVOID EXPOSED CIRCUITRY Do not touch any electronic circuitry inside of the product AVOID PINCH HAZARD A pinch hazard may exist between the pusher block and end blocks Avoid placing fingers between these points while the pump is running DO NOT OPERATE WITH SUSPECTED FAILURES If damage is suspected on or to the product do not operate the product Contact qualified service personnel to perform inspection ORIENT THE EQUIPMENT PROPERLY Do not orient the equipment so that it is difficult to operate the disconnection device PLACE PRODUCT IN PROPER ENVIRONMENT Review the operating manual for guidelines for proper operating environments OBSERVE ALL WARNING LABELS ON PRODUCT Read all labels on product to ensure proper usage E 72 EE E n lt gt A em gt KO CAUTION Protective Refer to Manual Ground Terminal HARV
49. eat to insert the Repeat Step function into the Method Select the Step s you would like to repeat from by selecting the Step button twice or selecting once and then choosing Accept HARVARD APPARATUS Step 1 Constant Rate 88 28 ml min Step 2 Ramp 170 2 nl min 88 28 ml min 6 NOTE This command creates a loopback to the s selected Step plus all subsequent Steps up Select desired step You can add delete move up down or set time delay Advanced Options Repeat Step screen Choosing Repeat brings you to a listing of the available steps in the Method Enter the number of repeats using the keypad then choose the Accept button to save your change and return to the Step Definition screen designated Step in a Method executing the to the point of insertion of the Repeat command For example if the Repeat command is set to repeat Step 2 one time the system will loop back to Step 2 and execute both Steps 2 and 3 one additional time Stop The Stop Command is used to instruct the pump to stop operation This Command can be used in conjunction with I O Events to control pump operation based on external events TO INSERT A STOP COMMAND IN A METHOD 1 From the Method Main screen choose Step Definition to enter the Step Definition screen Highlight select once the Method Step that you want the Stop Command to follow Choose the ADD STEP button to access the Method Steps Menu screen
50. en choose Accept to confirm your settings and return to the Ramp Definition screen Choose Accept again to return to the Step Definition screen _ Ramps lt 2 seconds from min to max rates may cause motor stalls phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 40 Gradient Profile TO SET UP EDIT A GRADIENT PROFILE The Gradient profile is used to combine multiple flow 1 From the Step Definition screen for Gradient streams from different pumps into a common output choose Set Combined Flow Rate to enter the stream The flow profiles can be constant ramped Set Rate screen or stepped Step 1 Gradient HARVARD APPARATUS Set Combined Flaw Rate Talie Set addresses and flow ercentage information Address Set percentage informatio of ii 50 Flow Address Rate E 50 Set Time amp S Steps 4 0 seconds Ramping 2 Enter the desired combined flow rate value for both pumps using the keypad or select the Minimum Rate or Maximum Rate button Time When creating or editing a Gradient profile you will define 4 primary operating parameters e The combined Flow Rate HARVARD APPARATUS Enter the rate and units The Target Starting and Ending Percentages for each pump in the system The Target Time that determines the total length of time over which the Gradient
51. ep Definition Below are some predefined steps including Constant Rate a single continuous rate of flow ny aa E Fy d 3 Ramped Rate a linearly changing flow rate pn n MEN between a starting and ending flow rte rot gt gt eae E Autofill continuous infuse withdraw or s withdraw infuse operation Gradient combined flow streams from multiple pumps into a common output stream In addition to these you can repeat steps or set a delay within a Method as well as send signals to external devices or react to signals received from external devices This package of pumping profiles and commands provides users with an extremely broad array of applications that can be easily configured with complex external programming Methods can be edited and saved under a new name simply edited to fit another application Steps can be copied moved inserted or deleted E 72 m EX n lt gt A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 33 Setting A Delay Quick Start HARVARD APPARATUS A Method Select You can set the Pump 11 Elite Series to run after a Quick Start Infuse Only delay This delayed run function allows you to set up all d mu E sure to Syringe Select the parameters for operation then have the pum
52. g on the mechanism any type of syringe glass plastic or stainless steel can be used The Pump 11 Elite can accommodate from 0 5 ul to 60 ml single syringe and 0 5 ul to 10 ml dual syringe The syringe barrel clamp should be flipped for syringes smaller than 30 ml For syringes larger than 30 ml the syringe clamp should be used as shown 1 Press the release button 1 and move the pusher block 2 to the approximate length of the syringe 2 Unscrew the bracket clamping knobs 7 8 to loosen the retaining brackets 4 5 on the pusher block 2 and the Syringe holder block 3 3 Unscrew the syringe barrel clamp by unscrewing the syringe barrel clamp knob 6 4 Place the syringe on the syringe holder block 3 Ensure the barrel flange is with in the syringe holder s block s retaining bracket 5 and the plunger flange is in the pusher block s retaining bracket The Pico Plus Elite can accomodate from 0 5 ul to 10 ml WT Product Overview 14 Screw down the syringe clamp knob 6 until the syringe barrel clamp 10 is tight against the syringe barrel Do not overtighten especially when using glass syringes Secure the syringe flanges by pushing the retaining brackets 4 5 tightly against the syringe flanges using the bracket clamping knobs 7 8 The syringe plunger should be secured by the pusher block retaining bracket and the flange of the syringe should be secured by the syringe holder block retaining
53. ge models These two models support infuse only withdraw only infuse withdraw and withdraw infuse Quick Start Methods with user defined flow rates and selectable target volume or target time values to control the total infusion time These models also support two user defined Methods up to 50 steps each Two Methods can be stored Pico Plus Infusion Withdrawal Programmable dual syringe only Supports all four Quick Start Methods and two user defined Methods up to 50 steps each phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Product Overview 11 PUMP 11 ELITE SERIES TOUCH SCREEN BUTTON REFERENCE The following buttons are part of the Pump 11 Elite Series software Settings Access the settings menu used to adjust the following parameters Audible Alarms Pusher Block Force Backlighting Baud Rate etc Page Down Used to page down in a display list Page Up Used to page up in a display list Fast Forward Advances the Pusher Block in the forward infuse direction Scroll Up Used to scroll up in a display list Fast Reverse Advances the Pusher Block in the reverse withdraw direction Scroll Down Used to scroll down in a display list Accept Used to accept the parameters settings on a screen and i Insert Step Used to insert a new Step in advance to next screen in
54. gers to create a complete Method The following sections describe each profile along with instructions for setting up the profile The following Pump Operation Profiles are available on the Pump 11 Elite Series CONSTANT RATE RAMP RATE Time Volume Cycles GRADIENT AUTOFILL Infuse Cycles Flow Rate or Total Volume Withdraw Time Volume Pump Operation Profiles available on the Pump 11 Elite Series m 72 EE E n lt D A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B E 72 EE EX n lt gt A em gt Ip HARVARD APPARATUS Operating the Pump 11 Elite Series Constant Rate Profile The Constant Rate profile is used to run the pump at a defined flow rate and for a specific pumping mode infuse or withdraw up to a target volume or for a defined time period Target Time or Total Volume Point Flow Rate Time Volume Constant Rate Pump Operation Profile When creating or editing a Constant Rate profile you will define three primary operating parameters The Flow Method defining the direction of flow infuse or withdraw The Flow Rate to define the rate of uptake or dispensing The Target Volume or Run Time that determine how long the pump will operate at the set rate When creating o
55. get volume BD plastic 60 ml 26 59 mm Infuse Rate Select 75 ml min Withdraw Rate Select 88 28 ml min Target Volume Select 60 ml Enter The Pump Password HARVARD APPARATUS Enter the Password up to 8 digits Pump Password LI Quick Start HARVARD APPARATUS Method Select Quick Start Infuse Withdraw s The screen is locked Press the Syringe Select lock button to unlock it BD plastic 60 ml 26 59 mm Infuse Rate Select 75 ml min Withdraw Rate Select 88 28 ml min Target Volume Select 4 60 ml pa phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Configuring the Pump 11 Elite Series Ke Setting the Force Limits Settings HARVARD APPARATUS The maximum linear force of the syringe pump is 35 Ibs TREE or 16 kgs This force is sufficient to damage delicate select the desired settings Audible Pump low volume syringes as well as many standard syringes MIT if pumping viscous fluids The of maximum force applied by the pusher block can be changed Force 100 spe In the settings screen press Force Backlight Address 0 TILT ror E Set Passw E ee D A minimum value of approximately 20 is required to overcome friction forces in the mechanism You may have to set the force level to a higher value depending on the back pressure friction in your system Set F
56. he pump to execute For example if you want to pump 100 ml using a 10 ml syringe the pump will execute 10 Infuse Withdraw or Withdraw Infuse cycles depending upon the chosen flow mode During an Autofill profile the Pump 11 Elite operates at a constant pump rate during the infusion and withdrawal stages although the rate can be different for each direction i e infuse vs withdraw E 72 m E n lt D A m gt Ip When creating or editing an Autofill Cycling profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Rate and Concentration screen HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B E 72 EE EX n lt gt A am gt AQ HARVARD APPARATUS Operating the Pump 11 Elite Series TO SET UP EDIT AN AUTOFILL CYCLING PROFILE 1 From the Step Definition screen for an Autofill Cycle choose the Set Mode button to toggle between the Withdraw Infuse WD INF and Infuse Withdraw INFAWD modes Withdraw Infuse begins by withdrawing into the syringe following by infusing into the target 2 Choose the Se
57. he optional RJ 11 T A range of values connections for daisy chaining the cr Carriage return connection from the computer must also come through the RJ 11 connectors lt la Line feed sp Space prefix Pump address prefix in the format NOTE Once communication is established _ If you manipulate the touch screen you must reestablish communications to the PC lt CR gt will do this NOTE System commands and start stop commands can be executed from Method or Method configuration screens However for parameter commands rate dia vol etc it is necessary to execute from the Quick Start configuration screen Using the Pump 11 Elite Series Pump Chain Commands The following instructions will help you to utilize the Pump Chain commands feature on the Pump 11 Elite Series pump Commands may be abbreviated to the first four letters i e address would be abbreviated addr A space must follow the command if arguments are included if the pump address is not zero prompt Prompt see below The following prompts are returned after a command is executed The pump is idle The pump is infusing The pump is withdrawing i The pump stalled T The target was reached If the pump is in poll mode an XON character is added after the prompt If the pump address is nonzero the pump address is prefixed to the prompt without a colon In the following list of commands The p
58. highlighted in magenta When you are done adding all required Steps to the Method choose the BACK button to return to the Method Main screen 5 phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m 72 EE EX v2 lt D A m gt TTS HARVARD APPARATUS Operating the Pump 11 Elite Series Modifying an Existing User Defined Method Once you have created a Method or have reviewed an Existing Method you may have a need to modify the structure of the Method i e the types of Steps and Commands included not just changes in the operating parameters for a specific Step The following procedures will help you to efficiently modify a stored Method CHANGING THE ORDER OF STEPS IN A METHOD 1 From the Method Main screen choose the Step Definition button to enter the Method Steps Summary screen HARVARD APPARATUS METHOD Select the desired syringe and define steps BD plastic 60 ml 26 59 mm Constant Rate AutoFill Ramp Repeat 2 Highlight select with a single touch the Step for which you want to change the order 3 Choose the Move Step Up or Move Step Down button as required to move the Step into the position that you want it executed HARVARD APPARATUS 88 28 ml min Select desired step You can add 170 2 nl min 88 28 ml min delete move up
59. information The two guide rods and the lead screw should be sparingly lubricated periodically every 100 hrs the pump will remind you The guide rods and the lead screw Chemical Compatibility Chart Chemical Concentration Acetonitrile Methyl alcohol Hydrochloric Acid Bleach Windex Isopropyl Alcohol Dish Soap Motor Oil Simple Green Gasoline Ethylene Glycol Antifreeze Mineral Oil Ammonia Magnalube Superlube Acetone Soda Coca Cola Sulfuric Acid Dimethyl Sulfoxide m 72 m E V2 lt gt A Gi gt En Sodium Hydroxide Nitric Acid Chloroform Appendices 66 should be lubricated with Super Lube Synthetic Grease provided with the pump To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the clear display window For more efficient cleaning use a soft cloth dampened not soaked with water an aqueous solution of 7596 isopropyl alcohol or a mild detergent Overlay Cover Overlay Adhesive gt gt U gt gt FOF E D U U gt Ege I NE Dg p ELI D E DE b E EE D U gt ON EN ELI DE D The chemical resistance data provided is for A Satisfatory reference only The data is based on the B Fair Minor Effects manufacturer s recommendations and is not a C Poor Moderate Effects guarantee of chemical compatibility for your test D Severe Effects conditions Wipe any spills immediately Not tested HARVARD phone 508 89
60. ion may not apply to you If a defect arises within the warranty period promptly contact Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 using out toll free number 1 800 272 2775 or outside the US call 1 508 893 8999 Email address is bioscience harvardapparatus com Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges for non warranty repairs Please allow a reasonable period of time for completion of repairs or replacement If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using a part number obtained from our Technical Support department If practical enclose a sample part or sketch We offer a complete reconditioning service Serial Numbers All inquiries concerning our product should refer to the serial number of the unit located on the rear panel E 72 EE EX n lt gt A em gt KO Calibrations All electrical apparatus are calibrated at rated voltage and frequency While the flow and volu
61. king on your computer on the hardware installation CD or on the Windows Update Web site with your permission 4 Can Windows connect to Windows Update to search for software Yes this time only Yes now and every time connect a device No not this time Click Next to continue lt Back Cancel Found New Hardware Wizard This wizard helps you install software for Syringe Pump E If your hardware came with an installation CD lt gt or floppy disk insert it now What do you want the wizard to do Install from a list or specific location Advanced Click Next to continue Appendices 62 1 On connection of the pump to the computer via USB the Found New Hardware Wizard will launch and ask how you wish to locate the driver Choose No not this time and click Next 2 Choose Install from a list or specific location Advanced and click Next phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 63 USB VIRTUAL COMMPORT DRIVER INSTALLATION CONTINUED 3 Browse for the proper USB driver installation folder in the USB folder and then click next It can be found on the CD supplied with the Pump 11 Elite Series Pump USB Driver WindowsXP or Vista USB Driver Windows2000 Found New Hardware Wizard Please choose your search
62. lee OPI essemus eemana donat san ennt epa Cines Ue tete RETEN ente Spes 21 Eor a CUSTOM MERERI RUDEM 21 LOCKING 1he TOUCH 6E oestevadeeedendtanee idrico En et EOE eE ev oci once 22 HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Table of Contents 3 SUBJECT PAGE NO SENO We ee 22 Turning the Touch Screen Lock Qn 22 Turning the Touch Screen Ree OFT sues sesccatas Pass Gus ad atatim dela pO aeo trame t asinis 22 Setting the Force Limits sesssssssse eene 23 Seting Display Backlight stecteraeactnretersevnteceenriertone enictoctten tendeasa tauren etenimedenetadtocentents 24 Setting the Pump Address 25 Setting the Baud Rate oo cccccc cece cceeeeeeeeeee eee nennen nennen 25 meris m Bo RUNDO PRI ER RET ES 26 Setting and Viewing Pump Information essssee seen 26 D T cess 27 Operating the Pump 11 Elite Series esse ss sees sese sees eenn eenn 28 CUNT Me PUMP NORRIS m 28 GUSTON Method VOKTO ST 29 Contouring CUsStom SOC eersten raden aE EE e aE NANen 31 Configuring 9 New Custom Meo sss vs vs x caos coaz 9 Sx utes ttes iab naO VR d 31 Configuring Custom Methods ssepe satestomttdresenitus oo Se ar vg Toa oaoa esaea 32 OS Oe DOY aeaee EEE E EAE
63. les button to enter the Set Total Volume or Cycles screen 13 Select either Total Volume or Cycles to define how the pump will control pumping activity Total Volume will cycle the pump the correct number of times to deliver the total volume according to the Volume per Cycle setting For example if you set a 10 ml Volume per Cycle and set 100 ml in Total Volume the pump will cycle 10 times 14 If you select Total Volume enter the volume desired using the keypad If you select Cycles enter the total number of Infuse Withdraw or Withdraw Infuse cycles that you wish to perform 15 If the Units of Measure associated with Volume are not correct for your application choose the Units button to enter the Units selection screen Select the unit of measure button ml ul nl pl appropriate for your application then choose the Accept button to return to the Set Target Volumes screen 16 When all parameters on the Set Total Volume or 4 Cycles screen are correct choose the Accept button to return to the Step Definition screen Using maximum rate may not be appropriate during the Withdraw stage of an Autofill profile since air can be drawn into the syringe or a high vacuum pressure can be produced depending on the syringe and or tubing being used or the fluid viscosity Incorporating Advanced Options Commands and I O Into A Method Pump 11 Elite Series Methods can incorporate a variety of commands that expan
64. lite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 5 u E D o i a E Q IWANVA ES ENIS HARVARD APPARATUS Operating the Pump 11 Elite Series STEP BY STEP OPERATION EXAMPLE USING A CUSTOM METHOD The following steps describe operation using a Custom Method In the following description we are assuming the use of a Method that is already saved on the Pump 11 Elite Series Note that if you make any changes to the Method s operating parameters and choose Accept on the page where the changes are made the changes automatically become part of the stored Method for future use 1 From the Method Quick Start screen the Method you wish to use should be displayed in the Method Select button If the Method is not displayed select the correct Method using the procedure outlined in Running The Pump 2 Choose the Syringe Select button to enter the Syringe Selection screen 3 Follow the instructions in the Syringe Selection section of this Guide to complete the selection of a syringe from the built in syringe definition list or to create a new syringe definition When finished choose the Accept button to save your changes or choose the Cancel button to cancel your changes and return to the Method Quick Start screen 4 Review the Pump Control Profile Steps described in the Step Definition button Note that the software will display the first three Steps in the Method i
65. locked Pressing the Stop Used to stop running a Method button twice locks the touch screen Tm E 72 EE E n lt gt A am gt AQ Touch Screen Locked ndicates that the Pump set up screens are locked from editing Press the button and enter minimum value allowed Clear Clears the counters timers File Options Access the File Options menu that is used to manage the list of Methods and to export Method information to an externally linked pump or to a connected computer Maximum Value Allowed Used to select maximum value allowed 1 DEDE E 0 HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 T3 EIN E marly D o i a uo IVANVW 5 9251 Selecting An Item From a Menu List Selecting items is easy with the Pump 11 Elite Series Touch the item to select highlight the item then touch again to accept it or press Canceling a Selection Canceling selections is easy i E Product Overview Syringe Selection Custom Syringe Air Tite Select an existing syringe HSW Norm Ject manufacturer or select Custom Becton Dickinson Syringe Glass all types Becton Dickinson Plasti pak Cadence Science Inc Micro Mate Glass Hamilton Glass all types Harvard Apparatus Stainless Steel HARVARD APP
66. me will stay calibrated the peak pressure may vary Harvard Apparatus recommends an annual calibration of the pump CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail biosclence harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m 72 m E V2 lt D A m gt EA Specifications Accuracy Reproducibility Syringes Min Max Flow Rate Minimum 0 5 pl syringe Maximum 10 ml syringe Maximum 60 ml syringe Display Non Volatile Memory Connectors RS 485 USB 1 O amp TTL Footswitch Linear Force Max Drive Motor Motor Drive Control Number of Microsteps per one rev of Lead Screw Step Resolution Step Rate Minimum Maximum Pusher Travel Rate Minimum Maximum Input Power Input Power Connection Power Supply Dimensions Weight Atmospheric Specifications Operating Temperature Storage Temperature Humidity Method of Operation Classification Pollution Degree Installation Category Supplier Name Supplier Address Supplier Phone Number Regulatory Certifications Safety Declarations EMC Declaration Pump 11 Elite 0 5 ss 0 05 0 5 ul 60 ml 10 ml dual 1 28 pl min 25 99 ml min 88 28 ml min 4 3 WQVGA TFT Color Display with Touch Screen Stores all settings IEEE 1394 6 pos Type B 15 pin D Sub Connector
67. mini phono jack 16 kg 35 lbs 10096 Force Selection 0 9 Stepper Motor Microprocessor with 1 16 microstepping 15 300 0 069 um ustep 27 5 sec ustep 26 usec ustep 0 15 um min 159 00 mm min 12 30 VDC 2 bmm ID x 5 5mm OD male plug 100 240 VAC 50 60 Hz 8 Watts Universal Power Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 22 6 x 17 78x 15 cm 9 x 7 x 6 in 2 1 kg 4 6 Ibs 4 C to 40 C 40 F to 104 F 10 C to 70 C 14 F to 158 F 20 to 80 RH non condensing Continuous Class 1 II Harvard Apparatus 84 October Hill Rd Holliston MA 01746 508 893 8999 CE ETL UL CSA WEEE EU RoHS amp CB Seheme ANSI UL 61010 1 2004 ED 2 Rev 2005 CAN CSA C22 2 No 61010 1 2004 Ed 2 IEC 61010 1 2001 Ed 2 Corrigendum 1 2002 Corrigendum 2 2003 CENELEC EN 61010 1 2001 CB Scheme CENELEC EN 61326 1 2006 General Information Pico Plus Elite 0 35 0 05 0 5 ul 10 ml 0 54 pl min 11 70 ml min N A 4 3 WQVGA TFT Color Display with Touch Screen Stores all settings IEEE 1394 6 pos Type B 15 pin D Sub Connector mini phono jack 16 kg 35 lbs 100 Force Selection 0 9 Stepper Motor Microprocessor with 1 16 microstepping 20 480 0 031 um ustep 27 5 sec ustep 26 usec ustep 0 02 um min 71 55 mm min 12 30 VDC 2 5mm ID x 5 5mm OD male plug 100 240 VAC 50 60 Hz 8 Watts Universal Power Supply Use Only a Harvard Apparatus Appro
68. mized with a small step angle stepping motor that drives a precision lead screw and pusher block The syringe mechanism is easy to use and securely holds the syringes for smooth flow performance HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B E 72 EE EX n lt D A am gt g HARVARD APPARATUS Easy external connections to a computer or other control devices are made through USB interface Simple ASCII commands make communication with the pump easy For direct control of the pump the user can use the I O interface 15pin Dsub One trigger output and a trigger input in for external events such as a process parameter are available The footswitch input will allow the control of the pump through an external device The unit also has an output for run indication allowing connection to a remote LED The Basic and Programmable 11 Elite Pumps are available with single and dual syringe options Complete flexibility is offered with the ability to use ANY manufacturers syringes ranging from 0 5 ul to 60 ml single syringe and 5 ul to 10 ml dual syringe The programmable models offer maximum flexibility and capability for configuring and running different Methods Methods can be configured and stored in the unit and recalled quickly with the touch of the screen The Methods
69. ml min Flow Direction gt Time Elapsed 00 00 00 Time Remaining 00 01 28 Total Inf Volume O ml RL Force Level 100 IVANVW 5 9251 Quick Start run screen with a Time Delay Set HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience pharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 34 Creating a New Custom Method If none of the Methods stored on the Pump 11 Elite Series match your requirements you can create a new Method and store the Method for use The following flow diagram outlines the basic steps you will follow to create a new Method Choose Syringe Select penetra Syringe Select Screen Y Choose Step Definition Method Selection Program Steps Summary Screen Y Add Step New Method Step Definition Screen Name Method Complete Parameter Entry Choose Accept Add More Steps Steps Summary Screen New User Defined Method creation workflow TO CREATE A NEW USER DEFINED METHOD ee 15 CHAR MAX 1 From the Method Quick Start or Method Main screen choose the Method Select button to Li w E Li T M IJ 1 LJ U enter the Method Selection screen a T Y 2 Select New Method then choose Accept or A E LJ F g H U L p
70. n screen 10 To preview the Method choose the Preview Method button or to run the Method choose the Method Run button HARVARD APPARATUS 4 stents Force Level 100 Syringe Ready to run method Step 1 Constant Rate Rate 88 28 ml min Target 15 secs Step 2 AutoFill 20 ml per Cycle Target 200 ml Total Volume Dispensed 0 ml E 4 GS D HARVARD APPARATUS 4 step s Force Level 100 Syringe BD plastic 60 ml 26 59 mm PT S Step 1 Constant Rate Rate 88 28 ml min Target 15 secs Step 2 Autofill 20 ml per Cycle Target 200 ml Step 2 Ramp Fy 170 2 nl min to 88 28 ml min for 5 secs Time Elapsed 00 00 19 Total volume Dispensed 23 16 ml 30 phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series KE Configuring Custom Methods The Pump 11 Elite Series Programmable Models offer the flexibility of configuring custom Methods including ings HARVARD APPARATUS multistep Methods The Methods and steps can also be f Change Logo triggered to start and stop with events and external I O Software t Select the desired settings and user defined inputs Audible Alarms Information To determine if your pump is programmable go NECI to the pump information screen The Model Type Debt cal should say Programmable Backlight 100 Address 0 TILT
71. n the main button area If the Method uses more than three Steps the last Step in the Method will be displayed in the box that is still part of the button beneath the Step Definition button 5 To review the Method Steps in detail or to update modify any of the stored Pump Control Profile Parameters choose the Step Definition button to enter the Method Steps Summary Screen The name of the Method is listed at the top of the screen and the complete list of Steps is listed in a series of buttons is on the left of the screen METHOD HARVARD APPARATUS Step 2 Ramp Select the desired step to repeat 170 2 nl rmin from Lid Step Definition Screen showing the list of Steps currently defined for the Method 6 To review details for any individual Step in the Method select the Step you wish to review then press the Step button again to enter the Step Definition screen for that Step 7 To edit the parameters for any Pump Control Profile follow the instructions supplied in the applicable Pump Operation Profile description contained in the Setting Method Parameters section later in this Guide 8 To return to the Method Steps Summary screen when finished reviewing or editing the Step parameter details choose the Accept button to save your changes or choose the Cancel button to cancel any changes you made 9 When you are finished reviewing the operating details for all Steps choose the Back button to return to the Method Mai
72. nc Engineering Formerly Popper amp gias Samers malina anii Sons MICRO MATE 1 ml 6 50mm 25bml 4 851 mm Glass 9 10 8 9 525 0 343 mm Si Di 10 00 20 19 13 0 485 IE ameter 12 60 50 28 6 0 728 0 2bml 3 47 mm 15 10 1 03 0 5 3 62 20 45 Air Tite 1 457 4 82 22 50 HSW Norm Ject 2 303 8 91 25 60 Size Diameter mud ae 4 606 mm 11 71 Natsume 1 ml 4 69 mm 14 65 l 7 284 Size Diameter 2 5 9 65 10 301 19 56 12 45 14 567 22 7 0 25 ml 2 5mm 15 9 23 28 02 0 50 20 05 27 5 1 a Becton Dickinson Hamilton Glass All Types Bean Dickinson Glass All Types Size Diameter Plasti pak Size Diameter 0 5ml 4 64 mm Size Diameter 0 5 ul 0 103 mm 5 Size Diameter 1 ml 4 699 mm o 11 86 1 ml 6 40 mm 8 585 0 343 14 34 2 5 9 30 11 989 0 485 19 13 13 10 14 427 0 729 22 7 15 3 19 05 1 02 28 6 21 0 21 59 1 457 23 0 Ca 26 594 2 304 Sherwood Mono 29 0 Un 26 594 d UE TI ACE lect NIPRO EU Terumo 5151 ud Plastic Size Diameter Size Diameter 7 285 Size Diameter 1 ml short 6 6 mm Y 1 ml 4 70 mm 10 3 Imi 4674mm 1 mllong 4 7 8 95 mm 14 567 83 865 2 5 ml 9 0 n 13 23 033 12 600 13 0 15 8 32 0578 15 621 15 8 gt 20 15 20 142 20 1 zu 23 1 23 571 29 2 all 29 26 568 29 1 Suggested Force Level Settings for Common Syringes Syringe Type Material Capacity Force Setting Common Manufacturer s Plastic Syringes lt 5ml 50 BD plastic Sherwood Airtite Terumo gt 5ml 100 Stainless Steel All 100 Harvard Apparatus Glass Glass 20ml 3096 Cadence Popper
73. of Run pensed is reached for the specified Mute All No audible sounds a Mute All m Qn EE E v2 lt D A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B D 3 5 u marly D o a a E uo IVWANVA S d4Sf HARVARD APPARATUS Configuring the Pump 11 Elite Series For Near End of Run Selection Select the volume dispensed For example if you select 80 the pump will sound an alarm 3 short beeps when 80 of the volume is dispensed Accept the changes made by pressing and go back to the Settings screen the changes made by pressing and go back to the Settings screen For a Custom Use this selection if the is not listed Press Set Other Value to enter a custom Enter the percentage Accept the changes made by pressing and go back to the Settings screen the changes made by pressing and go back to the Settings screen Near End of Run Select HARVARD APPARATUS Select an existing percentage or select Set Other Value EJ Near End of Run Select e se e o e se 80 HARVARD APPARATUS Select an existing percentage or select Set Other Value O 75 Set Other Value EI Set Near End of Run Alarm HARVARD APPARATUS Enter the percentage Percent Done 7
74. orce Level HARVARD APPARATUS O 100 Select an existing percentage or 90 Select the Force Level Accept the changes made by pressing and go back to the Set Force screen the changes made by pressing and go back to the Set Force screen SRL Diber vak Ed For a Custom Force 96 Press Set Other Value to enter a custom force Enter the precentage D 3 T EIN i marly D o i O a E Uo Set Force Level HARVARD APPARATUS Accept the changes made by pressing Force 100 and go back to the Set Force screen the changes made by pressing x and go back to the Set Force screen E m Hag J llls o oje US E Suggested Force Level Settings for Common Syringes Gl Syringe Type Material Capacity Force Setting Common Manufacturer s D Plasti 20ml 50 BD plastic Sherwood astic Syringes Airtite T gt 20ml 100 Irtite lerumo otainless Steel All 100 Harvard Apparatus lt 20ml 20 Glass Glass Cadence Popper 20ml 30 lt 1000uI 30 Glass Plastic lt 5ml 50 Hamilton SGE Sil 100 The Pump 11 Elite can accomodate syringes ranging from 0 5 pl to 60 ml single syringe and 0 5 ppl to 10 ml dual syringe NOI NOT The Pico Plus Elite can accomodate syringes ranging from 0 5 ul to 10 ml HARVARD phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com APPARATUS web Pump 11 Elite Series Syringe Pump User s Manual
75. oscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 60 PICO PLUS ELITE MIN MAX FLOW RATES Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on syringe manufacturer Maximum Rate Minimum Rate Units Syringe Size Syringe ID 0 5 ul 0 103 mm pl min nl min 1 pl 0 1457 mm pl min ul min 2 pl 0 206 mm pl min ul min 5 pl 0 343 mm pl min l min 10 pl 0 485 mm pl min l min 25 pl 0 729 mm pl min ul min 50 pl 1 030 mm pl min ul min 100 ul 1 457 mm pl min ul min 250 U 2 304 mm nl min pl min 500 ul 3 256 mm nl min pl min 1000 ul 4 608 mm nl min ml min 1 ml 4 699 mm nl min ml min 3 ml 8 585 mm nl min ml min 5 ml 11 989 mm nl min ml min 10 ml 14 430 mm nl min ml min m Qn EE E v2 lt gt A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail biosclence harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 61 APPENDIX C EXTERNAL CONNECTIONS User I O Connector Specifications resistor All Outputs are at TTL Logic Levels 1 Direction control input Rising edge sets pump to infuse l Trigger 1 Out Footswitch In Falling edge sets pump to refill trigger In 2 Trigger Input Direction In Event trigger falling edge triggers a Method b
76. p start BUR IU REN unattended after a specified amount of time Infuse Rate Select 75 ml min SETTING A DELAY FOR METHOD OPERATION Target Volume Time Select 60 ml 1 From the Quick Start or Method Main screen choose the Set Delay Parameters button to enter the Set Delay 2 Enter the desired Time using the keypad Method Main HARVARD APPARATUS 3 Ifthe Units of your Time are not correct for Select the desired syringe and define steps your application select the Units button to S Syringe Select toggle between sec seconds and hh mm ss hours minutes seconds To use hh mm ss option enter your time value in hours Step Definition minutes and seconds separating each value Step 1 Constant Rate with a colon You can enter a value up to Step 2 AutoFill 99 99 99 Step 3 Ramp Step 4 Repeat 4 When the desired values are entered choose the Accept button to save your Changes and return to the Method Quick Start screen When a Time Delay value is active the Time Delay Parameters button is displayed in Red on the screen to alert Set Delay HARVARD APPARATUS the user that a delay has been set Time EE he time delay 5 Press the Run button to activate the Delay E dg SIG EJ Set Delay Time screen D 3 T3 EIN M marly D o i a uo Run HARVARD APPARATUS Infuse Withdraw BD plastic 26 59 mm 60 00 ml Target 60 ml Infuse 75 ml min Withdraw 88 28
77. profile will be calculated 88 28 ml min The Number of Steps that will be created in the profile D 3 T EIN i marly D o a a E uo LI See Appendix C RS 485 for physical gradient connections 3 If the Units of Rate are not correct for your application choose the Units button 4 On the Units Selection screen select the unit Master address must be set to 00 Ensure that the satellites address matches the address set in the gradient step of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 5 When all parameters are correctly set on the Set Rate screen choose Accept to confirm CAUTION ALL PUMPS MUST USE your settings and return to the Step Definition THE SAME SYRINGE SIZE AND screen TYPE IF THERE IS A SYRINGE 6 Choose the Set Percentage button for pump 00 CHANGE AT MASTER THEN SAME CHANGE MUST OCCUR AT ALL 7 Set the Start and End Percentages for pump 00 IWANVA S d4sfn then choose Accept SATELLITES 8 Choose the Address button and then set the address of the second pump Choose Accept to return to the Step Definition screen NOTE Make all cable connections then power up the satellite pump before powering up the eee master pump Set Pump Number Pump number o ziee nii EJ EJ HARVARD phone 508 893 8999 fax 508 429 5732 email bio
78. r editing a Constant Rate profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse and Withdraw Rate screens HARVARD APPARATUS Enter the step settings 0 ml min Constant Rate Profile Setup Screen 1 10 11 12 13 38 TO SET UP EDIT A CONSTANT RATE PROFILE From the Step Definition screen for a Constant Rate profile choose the Set Mode button to toggle the Flow Method between Infuse and Withdraw operation Choose the Set Rate button to enter the Set Rate screen Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of v4 time sec min hr as appropriate then choose ACCEPT to confirm your selections When all parameters are correctly set on the Set Rate screen choose ACCEPT to confirm v your settings and return to Constant Rate Definition screen Choose the Set Target Volume or Time button to enter the Set Volume or Time screen On the Set Volume or Time screen select the Volume or Time button as desired
79. racters maximum 5 When finished entering the new Method Name choose Accept to save your changes E 72 EE EX n lt gt A Gi gt Selection screen 2 Renaming A Method Follow these instructions to rename a previously saved Method s 1 From the Quick Start screen choose the Method Select button to enter the Method selection screen 2 From the list of available Methods select 4 press once the Method you want to rename then choose the File Options button to enter the File Options screen HARVARD APPARATUS web WWW harvardapparatus com describes the file management facilities available on the 5 The Method management procedures 6 outlined in this section are not available for 1 enter the File Options screen 4 Name Entry screen The current Method Name 5 button 123 to display the list of available 6 Operating the Pump 11 Elite Series 4 3 Choose Rename Method to access the Method Name Entry screen The current Method Name will be displayed in the text entry box On the Method Name screen type the new name for your Method using the onscreen keyboard Choose the Symbols Numbers button 123 to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters Method choose the Letters button ABC 15 characters maximum When finished entering the new Method Name choose Accept to save your change
80. reached and if not Status Serial Command Notes The output is in three integer fields and one flag field all separated by spaces and terminated by a carriage return linefeed pair The first integer is the current rate in femtoliters per second The second integer is the infuse time in clock cycles One clock cycle is 1 56 000 000 second The time has a granularity of 1 millisecond The third integer is the infused volume in femtoliters All three values are for the current direction The flag field consists of six flags Flag one is the motor direction and will be I if the pump is infusing and w if the pump is withdrawing If the letter is lower case the pump motor is idle If upper case the pump motor is running Flag two is the limit switch status If the infuse limit switch was hit I is displayed If the withdraw limit switch was hit W is displayed If no limit switch was hit or the pump does not have limit switches is displayed E 72 EE E n lt gt A m gt TTS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 56 Appendices APPENDIX A SYRINGE VOLUME DIAMETER REFERENCE TABLE Harvard Apparatus SGE Scientific Cadence Science Hoshi Stainless Steel Glass I
81. ress New Method again v G EAIESIEAIEAILIILIILUIE New Method Name Entry screen showing letter keys To view numbers symbols choose the REY use button in the lower left corner 3 On the Method Name screen type the name of your Method using the onscreen keyboard Choose the Symbols Numbers button KES to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters Method choose the Letters button ABC n T E V2 lt gt A Gi gt TS HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m Qn m E v2 lt gt A m gt AQ HARVARD APPARATUS Operating the Pump 11 Elite Series TO CREATE A NEW USER DEFINED METHOD CONTINUED 4 When you have entered the desired name for your Method choose the Accept button to go to the Method Main screen Your new Method will now be listed in the Method Select button 5 Choose the Syringe Select button and complete the description of the syringe by following the procedure outlined previously in the Syringe Selection section of this Guide 6 To begin creating Pump Control Profile Steps choose the Step Definition button to enter the Method Steps Summary screen 7 To add a Step to your Method choose the Add Step button to access the Me
82. ress of 0 are masters and pumps with an address between 1 and 99 are slaves Query response lt lf gt Backlight is set to lt cr gt lt lf gt lt prompt gt HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m Qn EE EZ v2 lt D A am gt AQ HARVARD APPARATUS Operating the Pump 11 Elite Series ECHO Sets or displays the USB echo state Valid states are on or off Command format echo onloff Query response Ift HE Echo is OFF lt cr gt If B prompt Or lt lf gt Echo is ON lt cr gt If prompt FORCE Sets or displays the infusion force level in percent Valid range is 1 to 100 Command format force 1 100 Query response If 285 496 cr If prompt METRICS Displays the pump metrics The operation of this command is covered in the 11 Elite Configuration document Command format metrics POLL Sets or displays the polling mode state In polling mode prompts are inhibited if they aren t generated by a typed command Valid states are on or off Command format poll onloff Query response lt lf gt Polling mode is OFF lt cr gt lt lf gt lt prompt gt or lt lf gt Polling mode is ON lt cr gt lt f gt lt prompt gt od TIME Sets or display
83. s and return to the File Options screen Selection screen Choose BACK to return to the Method E n sci m Appending A Method Follow these instructions to Append a previously created Method into a new Method From the Method Main or Quick Start screen choose the Method Select button to enter the Method Selection screen From the list of available Methods select press once the Method you want as the base Method Choose the File Options button to enter the File Options screen Choose Append to access the Method to Append screen Choose press twice the Method you would like to Append attach to your base Method The pump will return you to the File Options screen and display a confirmation that the Method was copied Choose BACK to return to the Method Selection screen Deleting A Method Follow these instructions to delete a previously saved and return to the File Options screen Method 6 Choose BACK to return to the Method 1 From the Method Main or Quick Start screen choose the Method Name button to enter the Method Selection screen From the list of available Methods select press once the Method you want to delete then choose the File Options button to enter the File Options screen Choose Delete Method The application will display a warning message in the Message Area asking you to confirm your deletion request Choose Delete Method again to confirm the deletion and
84. s Information In the Settings screen select Power Up Runnin F Baud Rate p g Force 100 a Accept the changes made and go back Backlight 100 Address 0 TILT Cw d to the previous screen before Settings Method was selected Power Up Set Password Running Cancel and go back to the previous ___ ain ae screen before Settings Method was selected NOTE Elapsed Timers and Total Infused Volume will be reset when power resumes Counters and timers information prior to loss of power are lost WE This feature is only valid in Infuse Only or _ Withdraw Only Methods This feature will s only execute if the pump is running at the time when power ts lost and was running with no target set Setting and Viewing ings HARVARD APPARATUS Pump Information TEE Change Logo Software i Select the desired settings The information on the pumps parameters are displayed ES f f udible ump in the Pump Information Screen Alarms Information A In the Settings screen select Pump Information Force 100 The current information on the pump will be displayed Backlight 100 TT The Model type SE Set Password e The Software Version E D e The Maintenance Due D 3 5 u marly D o i a E Q The Current Pump Address Press Maintenance Reset to reset the of hours Pump Information HARVARD APPARATUS for maintenance This will reset it to 100 hours Model Pump 11 Type
85. s seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon You can enter a value up to 99 99 99 7 When your Time entry is correct choose the Accept button to insert the Delay Step in the Method and return to the Step Definition screen Repeat Step The Repeat Command is used to repeat Steps in a procedure a defined number of times To use this Command you will tell the application what Steps you want to Repeat and how many times you want them repeated The Repeat Step command creates a jump back to a specific Step in a Method that executes the defined Step plus all following Steps up to the Repeat command i e it creates a Method loop When defining a Repeat jump in a Method the Command targets the Step sequence position and not the function that currently occupies that position For example if you create a Repeat jump to Step 4 in a Method currently a Ramp Pump Operation Profile and subsequently move a Constant Rate profile into the Step 4 position the Repeat jump will then connect to the Constant Rate operation and not to the Ramp operation 44 TO INSERT A STEP REPEAT SEQUENCE IN A METHOD 1 From the Method Main screen choose Step Definition to enter the Step Definition screen Highlight select once the Method Step that you want the repeat step to follow Choose the ADD STEP button to access the E Method Steps Menu screen Choose Rep
86. s the date and time Command format time mm dd yy hh mm ss Query response If HE HHERHEHHE HH HH HH SM lt cr gt lt lf gt lt prompt gt 24 hour time format VER Displays the short version string Command format ver Query response lt lf gt 11 Elite lt cr gt lt lf gt lt prompt gt VERSION Displays the full version string Command format version Query response lt if gt Firmware v 4 lt cr gt lt lf gt RTOS FreeRTOS v http www treertos org lt cr gt lt lf gt Pump address lt cr gt lt lf gt lt prompt gt phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B m Qn EE EZ v2 lt gt A m gt Ip HARVARD APPARATUS Operating the Pump 11 Elite Series Run commands IRUN Runs the pump in the infuse direction Quick Start mode only Command format irun rrun Runs the pump in the opposite direction Quick Start mode only Command format rrun RUN Simulates a key press of the run button located in the lower right corner of the screen Command format run STOP STP Stops the pump Command format stop stp WRUN Runs the pump in the withdraw direction Quick Start mode only Command format wrun Rate commands CRATE Displays the current rate that the motor is r
87. science harvardapparatus com APPARATUS web HARVARD APPARATUS Enter the pump address Enter O to remove a pump Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 41 9 The pump will calculate the remaining Running a Gradient Profile percentages for the second pump The pump control button s background color indicates 10 Choose the Set Time and Steps button Enter the status of the satellite unit the total amount of time and the number of steps desired and then choose Accept Blue Background Connected Motor Stopped Red Background Comm Error Yellow Background Motor Stall Green Background Motor Running HARVARD APPARATUS HARVARD APPARATUS Enter the total time and number Number of Steps o of steps 1 steps Force Level 100 BD plastic 60 ml 26 59 mm Method started U Pump Control button showing the tellite Time Elapsed 00 00 01 Rer ES Total Volume Dispensed 0 ml address For a Constant rate or Ramp 7 profile When running a Gradient step the satellite choose 0 steps For a Stepped pumps screen will display Satellite profile L select the number of steps followed by its pump address A stop button desired will be active on the satellite when the pump is running When running a gradient step ensure that no unused pumps are included in the pump chain m n m E V2 lt D A m gt TTS HARVAR
88. sing drop down list Click OK If the Virtual Comm Port is not known use Device Manager to find it Instructions on setting up the Virtual CommPort Driver are supplied in Appendix E Connect To S Pump 11 Elite Enter details for the phone number that you want to dial E 72 m E n lt D A em gt En Country region ied States 1 Area code 5E Phone number Connect using iv To determine the proper comport in your PCs Systems Properties screen under the Hardware tab select Device Manger Expand the ports COM amp LPT section and you should see Syringe Pump The comport is listed next to this in parenthesis Operating the Pump 11 Elite Series 50 4 Set up the Port Settings as shown below and click OK COMG Properties E x Port Settings Bits per second 115200 Data bits e lt Parity None Stop bits 2 lt Flow control N one lt Restore Defaults Cancel Apply 5 Verify the Settings are as shown below PHD Ultra Comm Properties xj Connect To Settings Function arrow and ctrl keys act as E Terminal keys C Windows keys Backspace key sends Cu amp H C Del C CH Space Ctrl H Emulation auto detect Terminal Setup Telnet terminal ID AN SI Backscroll buffer lines 500 ES Play sound when connecting or disconnecting Input Translation 6 Choose ASCII Setup
89. stic 26 59 mm 60 00 ml Target 60 ml Infusion started Infuse 75 ml min Withdraw 88 28 ml min Flow E Direction gt K EET Time Elapsed 00 00 05 Time Remaining 00 01 23 Total Inf Volume 6 513 ml Force Level 100 phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com web Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series 29 Custom Method Workflow The following flow diagram describes the use of a Custom Method Select Method Select Syringe Manufacturer amp Size OR Create New Syringe Type Review Method Steps Pump Operation Profiles Enter Pump Operation Profile Parameters Validate Commands and UO Operations The software provides the user with a variety of prompts and warnings to help guide the setup of Methods For example if a pump Method incorporates steps that exceed the capacity of the defined syringe the system will prompt you that parameters are out of range The system also prompts users if required values are not entered The pump will check the validity of parameters entered for each Step in the Method but will not review the Method in its entirety to verify that it is logical and can be fully executed m 72 EE EX v2 lt gt A Gi gt KO HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience Qpharvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 E
90. t lt sp gt lt sp gt error message lt cr gt Command founat If B prompt cat Query response Argument Errors lt if gt Method name Size cr Argument errors are displayed when a command If lt cr gt argument is unrecognized or out of range The argument in question will be displayed except in the case of missing arguments lt lf gt Method name lt cr gt This line is repeated for each Tile on the disk l lt lf gt lt lf gt file s using steps lt cr gt The argument error has the following format lt lf gt lt prompt gt lt lf gt Argument error bad argument lt cr gt ea ror aes auee Notes The file size is the number of steps in the a P P 9 Method plus one for the file information header If B9 prompt l The last line shows the number of files and the total number of steps being used on the disk System commands DELMETHOD ADDRESS Deletes a Method stored in the pump Sets or displays the pump address Valid range is 0 to Command format 99 delMethod Method Command format DIM address 0 99 Sets or displays the backlight level in percent Valid range is O to 100 Note that specifying O will turn the Query response Mn backlight off so that the screen will not be visible m Qn EE EZ v2 lt gt A am gt AQ lt lf gt Pump address is cr Command format lt lf gt lt prompt gt dim 0 100 Notes Pumps with an add
91. t Rates button to enter the Set Infuse Withdraw Rate screen 3 Select the Infuse Rate it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired rate using the keypad or select the Minimum Rate or Maximum Rate button 4 If the Units of Rate are not correct for your application choose the Units button 5 On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 6 Select the Withdraw Rate and enter the desired rate using the keypad or select the Minimum Rate or Maximum Rate button 7 When all parameters are correctly set on the Set Infuse Withdraw Rate screen choose Accept to confirm your settings and return to Step Definition screen 8 Choose the Set Volume per Cycle button to enter the Set Volume per Cycle screen 9 Enter the Volume using the keypad 10 If the Units of Measure associated with Volume are not correct for your application choose the Units button to enter the Units selection screen Select the unit of measure button ml ul nl pl appropriate for your application then choose the Accept button to return to the Set Target Volumes screen 11 When all parameters on the Set Volume per 43 Cycle screen are correct choose the Accept button to return to the Step Definition screen 12 Choose the Set Total Volume or Cyc
92. t the signal will be sent out of and whether it will be high or low Low Signal BE 1 0 OUT Trigger Advanced Options menu The following option is available when setting an I O OUT Trigger command Trigger 1 Specifies output via the Trigger 1 Pin 4 port When selected you must also specify whether the signal to be sent is High Signal or Low Signal High Signal Sets the output signal to logic high Low Signal Sets the output signal to logic low 45 TO INSERT AN 1 0 OUT TRIGGER COMMAND From the Method Main screen choose Step Definition to enter the Step Definition screen Highlight select once the Method Step that you want the I O OUT Trigger to follow Choose the ADD STEP button to access the Method Steps Menu screen Choose the O OUT Trigger button to enter the Trigger Step Definition Screen Set your Trigger settings by using the following procedure a To send a Trigger signal to an external device via Pin 4 select the Trigger 1 button Then select whether the signal will be High or Low by selecting the appropriate button selection will highlight in white Choose the Accept button to save your changes and return to the Step Definition screen When using triggers the first step in your Method should define the trigger starting level High or Low You may then use the trigger command to change the level as desired for your Method phone 508 8
93. the Pump 11 Elite Series dU Choose the Set Start and End Rate button to enter the Set Start amp End Rates screen HARVARD APPARATUS NN Xm Enter the rates and units 7 s 9 Z 88 28 ml min 170 2 nl min Set Start and End Rates screen Select the Set Start and End Rate button it should be selected by default as indicated by the active data entry box to the right of the button Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose ACCEPT to confirm your selections Repeat Steps 3 to 5 for the End Rate by selecting the End Rate button and continuing data entry When all parameters are correctly set on the Set Rate screen choose ACCEPT to confirm your settings and return to Ramp Definition screen Choose the Time button to enter the Set Time screen Enter the desired Time value using the keypad If the Units of Time are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon When all parameters are correctly set on the Set Target Time scre
94. the menu a Method while editing creating the Method The new Step is inserted after the Step that is currently selected 2 D 2 Q Cancel Used to cancel any changes entries on a screen and return to the previous screen in the menu Delete Step Used to delete the selected Step in a Method while editing creating the Method To confirm deletion you must select the Delete Step button a Set Delay Parameters Used to set the second time time values for delayed operation This permits the pump to start automatically after a preset time La Back Used to go back one screen aH EJ E3 FX E Move Step Up Voves the currently selected Step in a Method up one position in the Method Order e g Step 3 becomes Step 2 m Set Delay Parameters ndicates the Delay Timer has been set The pump will start automatically after the preset delay expires led Move Step Down Moves the currently selected Step in a Method down one position in the Method Order e g Step 2 becomes Step 3 Ed Run Preview Access the Method Preview screen that shows a summary of steps and operating parameters Copy Step Creates a duplicate copy of Run Resume Used to Run or Resume a the currently selected Step in a Method Method and inserts it as the last Step in the Method being created edited Re Run Clears counters timers and runs the selected Method from the beginning Touch Screen Unlocked ndicates that the Pump is un
95. thod Steps Menu screen The available options include Constant Rate Ramp I O in Event Gradient I O out Trigger Autofill i Stop Repeat Delay HARVARD APPARATUS 88 28 ml min 170 2 nl min 88 28 ml min Select desired step You can add delete move up down or set time delay From Step 1 3 times Method Steps Profile Menu screen 8 Choose the Method Step type that you want to add to the Method If you choose a pumping profile e g Constant Rate Ramp etc you will be taken to the Step Definition screen for the selected profile 9 10 11 12 oO Complete the parameter entry applicable to the selected Pump Operation Profile follow the detailed instructions supplied in the Pump Operation Profiles Existing and User Defined Methods section later in this Guide _ Ifyou do not complete the required parameter entry for a Step subsequent Step additions may be ignored You should enter all required parameters for a Step when creating it to ensure that the application software can assemble your Method without errors When you have completed all parameter entries on the Step Definition screen choose the ACCEPT button to save your changes and return to the Method Steps Summary screen Repeat items 7 10 in this procedure to include additional Steps in the Method When adding a Step to a Method the Step is added after the currently selected Step i e the Step that is
96. ump address is prefixed to every response line followed by a colon e lt lf gt lt prompt gt is the response unless otherwise noted phone 508 893 8999 fax 508 429 5732 email bloscience pharvardapparatus com web WWW harvardapparatus com HARVARD APPARATUS Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series D Error Messages BAUD Sets or displays the baud rate Valid baud rates are 9600 19200 38400 57600 115200 128000 230400 256000 460800 and 921600 Note that some computers may not be able to handle baud rates above 115200 Error messages are displayed if the entered command cannot be executed for some reason The error message will take up two lines with the first line being the message type and the second line describing the error itself The second line may be up to 80 characters long Command format baud 9600 19200 38400 57600 115200 Command Errors 128000 230400 256000 460800 921600 Command errors are displayed when the command is unrecognized entered in the wrong mode or the state of the pump keeps the command from executing i e lt lf gt baud lt cr gt using the IRUN command If a limit switch is active Query response lt lf gt lt prompt gt The command error has the following format CATALOG If tt Command error lt cr gt Displays a catalog of Methods stored in the pump lt lf gt lt sp g
97. unning at A valid response is returned only in dynamic situations while the pump is running Quick Start mode only Command format crate Query response If HE Infusing at xl xxx cr lt lf gt lt prompt gt Or lt lf gt Withdrawing at xl xxx lt cr gt If prompt 54 DIAMETER Sets or displays the syringe diameter in mm Quick Start mode only Command format diameter syringe diameter Query response lt it gt AHHH mm cr lt lf gt lt prompt gt IRAMP Sets or displays the target infusion rate while ramping Quick Start mode only Command format iramp start rate start units end rate end units ramp time in seconds Query response lt lf gt Ramp not set up lt cr gt lt lf gt lt prompt gt Or lt lf gt Starting rate xlxxx cr lt lf gt Ending rate xl xxx cr lt lf gt Ramp time seconds cr lt lf gt lt prompt gt IRATE Sets or displays the infusion rate Quick Start mode only Command format irate rate rate units max min Query response lt lf gt xI xxx lt cr gt lt lf gt lt prompt gt phone 508 893 8999 fax 508 429 5732 e mail bioscilence harvardapparatus com web WWW harvardapparatus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Operating the Pump 11 Elite Series DD WRAMP CVOLUME Sets or displays the target withdraw rate
98. ved Power Supply and Line Cord 22 6 x 17 78 x 15 cm 9 x 7 x G in 2 1 kg 4 6 lbs 4 C to 40 C 40 F to 104 F 10 C to 70 C 14 F to 158 F 20 to 80 RH non condensing Continuous Class 1 II Harvard Apparatus 84 October Hill Rd Holliston MA 01746 508 893 8999 CE ETL UL CSA WEEE EU RoHS amp CB Scheme ANSI UL 61010 1 2004 ED 2 Rev 2005 CAN CSA C22 2 No 61010 1 2004 Ed 2 IEC 61010 1 2001 Ed 2 Corrigendum 1 2002 Corrigendum 2 2003 CENELEC EN 61010 1 2001 CB Scheme CENELEC EN 61326 1 2006 HARVARD phone 508 893 8999 fax 508 429 5732 email bioscience harvardapparatus com APPARATUS web WWW h arva rd a D pa rat us com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Product Overview Product Overview The Harvard Apparatus Pump 11 Elite Series is the Advanced microstepping techniques are employed to next generation of syringe pumps The series offers further reduce the step angle to eliminate flow unparalleled ease of use through the high resolution pulsation The accuracy is 0 596 0 3596 for Pico Plus color touch screen user interface The full touch screen Elite A wide dynamic flow range from 1 28 pl min to interface enables the user to quickly create 88 28 ml min 0 54 pl min to 11 70 ml min for the Pico configurations and recall them for easy use The TFT Plus Elite can be programmed into the pump Flow color display presents all the pump operating r
99. y chain apply power to the last group and work backwards When removing power be sure to remember that power removed from a stand alone unit will also cause power to be removed from all RS 485 powered units deriving power from it HARVARD phone 508 893 8999 fax 508 429 5732 e mail biosclence harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B Appendices 65 Legacy RJ 11 Option Transmit Ground Receive Transmit Ground Receive Port 2 Port 1 out in Pumps with Legacy RJ 11 Option RJ 11 PUMP HJ 11 RJ 11 PUMP RJ 11 PUMP IN ADDR OUT N ADDR1 OTT IN ADDRn D Sub 9 F to Non Standard Mon Stangard Telephone Telephone Cable Cat CableCat 70 2022 55 7760 or 72 2478 Telephone Cable Cat 55 7760 or 72 2478 Daisy Chaining with Legacy RJ 11 Option Pumps 1 n also have RJ 11 telephone jacks m Qn EE E v2 lt gt A m gt Ip HARVARD phone 508 893 8999 fax 508 429 5732 e mail bioscience harvardapparatus com APPARATUS web www harva rda ppa ratus com Pump 11 Elite Series Syringe Pump User s Manual Publication 5420 002 Rev B APPENDIX D MAINTENANCE Maintenance Pump 11 Elite Series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material Refer to the chemical compatibility chart below for specific chemical resistance
100. yringe Pump Series Model No Pump 11 Elite Catalog 70 45xx To which this declaration relates is in conformity with the applicable EC Directives harmonized standards and other normative requirements Application of Council Directive s 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive 2002 95 EC RoHS Directive Standard s to which conformity is declared Safety EN 61010 1 2001 2nd Edition Emissions Immunity EN 61326 2006 05 01 EN 61000 4 2 1995 A1 1998 A2 2001 EN 61000 4 3 2002 EN 61000 4 4 2004 EN 61000 4 5 1995 A1 2001 EN 61000 4 6 2007 EN 61000 4 8 1993 A1 2001 EN 61000 4 11 2004 EN 61000 3 2 2000 EN 61000 3 3 1995 A1 2001 CISPR11 2003 A1 2004 Group 1 Class B FCC Part 15 Class B EMC and Safety compliance were evaluated by Intertek ETL Semko Reference test report file numbers 100071222 BOX 002 004a 007 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date April 22 2010 C Signature HARVARD E APPARATUS VP Engineering Operations Position Harvard Apparatus pumps are distributed on a non exclusive basis by I 1S TECH Instech Laboratories Inc Headquarters 5209 Militia Hill Road Plymouth Meeting PA 19462 TL 800 443 4227 TL 610 941 0132 FX 610 941 0134 European sales office P O Box2 9950 AA Winsum Th

Harvard Apparatus Pump 11 Elite

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