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Microplate Washer LP40/LP41 User manual

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1. Set Method interval with T or and the gt keys from 0 min 0 s up to 59 min 0 s DISPENSING YES Go to page 44 43 3 7 PROGRAMMING continued 3 7 4 ADD continued 3 7 4 1 ADD KIT continued Continued from page 43 3 7 4 2 ERRORS in KIT programming ERR 24 TOO MANY KIT PROGRAMMED YES ERR 32 KIT IMPOSSIBL Answer YES when no more methods ha ve to be added Select the Nr of times the kit has to be run with T or U keys up to 9 times When run more than once set Kit interval with T or and gt keys from 0 min O s up to 59 min 59 s The new kit is added to the end of the existing kit list Display is back to main menu 75 kits are already programmed You must first delete an existing kit programming a new one The name or the kit name you have entered is already existing Enter a new name to conti nue kit programming Memory is full because one or several kits are containing a large number of methods You must first delete an existing kit prior programming a new one 44 3 7 PROGRAMMING continued 3 7 4 ADD continued 3 7 4 3 ADD METHOD The method is added after the last method of the selected kit Use T and keys to scroll the available menu Press
2. Length Height Weight without accessories Operating conditions 368 mm LP40 683mm LP41 530 mm Plate carrier out LP40 amp LP41 483 mm LP40 443 mm LP41 11 0 Kg LP40 11 6 Kg LP41 15 30 C 15 85 R H 1 7 3 Hardware specifications Manifold LP40 LP41 Vacuum power LP40 LP41 Volume of WASTE bottle LPAO LP41 User interface 8 or 12 channel 8 2 8 3 8 12 or 2 12 channel 1 integrated vacuum pump maxi 8 L min 2 integrated vacuum pumps maxi 16 L min 2 litres 8 litres Flat keyboard with 5 diaphragm keys 2x20 characters LCD screen backlighted on LP41 13 1 7 Technical features continued 1 7 4 Software specifications Kits Method Soak time Repetition Wash mode Omit strip Plates Other features Up to 75 washing kits programmable Each kit is saved under its own name up to 15 characters and is a combination of methods repeated or not with or without soaking times in strip mode or in plate mode 6 single cycle washing methods Wash Aspiration Dispensing Bottom Washing Bottom Aspiration Agitation 4 two cycle washing methods Wash Aspiration Wash Bottom Aspiration Bottom Wash Aspiration Bottom Wash Bottom Aspiration 0 to 9 9 sec Strip mode 0 to 59 min 59 sec in Plate mode Every method can be repeated from 1 up to 9 times Strip mode or Plate mode Strips can be omitted at kit programmation Up to 10 plate
3. gt mz gt Ja msjm gt Method Code nW NW nw Nwta nW nA nD nw na nAg A a A Kit Parameter Value See def MODE STRIP or p 16 j i 3 gt y 8 i 2 7 PLATE CROS ASP YES or NO p 18 ii k x ASP TIME 0 1 to9 9s p 5 18 VOLUME 50 to 3000 ul p 19 i 5 ii OVERFLOW 1 0 to 12 9 mm p 19 s LIQUID Wash W1 to p 19 x x 3 Wash W9 FLOW 5 to 5 p 19 BOT WASH 10r2 p 22 NUMBER BOTTOM TIME 0 1t09 9 s p 22 E BOT ASP 10r2 p 23 3 NUMBER SHAKE TIME 7 to59 9s D 25 No OF CYCLES 1 9 p 17 SOAKING 0to 59 min in P 17 li d n E b x t PLATE mode 0 to 9 9 s in STRIP mode 3 7 4 4 ERRORS on METHOD programming Memory is full because one or several kits are containing a large number of methods You must first delete an existing kit or existing method prior programming a new one 46 3 7 PROGRAMMING continued 3 7 4 ADD continued 3 7 4 5 ADD PLATE ADD PLATE 7 4 YES ESC BOT SHAPI IN 2 0mm OUT 2 0mm ASP HOR POS 1 i T POS 13 5mm V YES ESC Go to page 48 The plate is added at the end of the existing plate list Use 1 and J keys to scrol
4. Back to kit edition see 3 7 7 1 56 3 7 PROGRAMMING continued 3 7 7 EDIT continued 3 7 7 2 EDIT PLATE y BOT T SHAPI i ES Select plate to be edited Every parameter of the selected plate can be modified by using M or keys Press YES to validate main pa rameters modification A Back to main menu see 3 3 57 3 7 PROGRAMMING continued 3 7 8 COPY 3 7 8 1 COPY KIT KIT KIT Nr 24 Select kit to be copied Select kit before which you want to pla ce copy Enter copy name select character with 1 and keys Then press key to move to the next character up to 15 cha racters ERR TAr UR uq No kit programmed PROGRAMMED Tr You must first ADD a kit See also ERR 24 ERR 25 ERR 32 On 8 3 7 4 2 58 3 7PROGRAMMING continued 3 7 8 COPY continued 3 7 8 3 COPY PLATE Select the plate to be copied Select the plate before which want to place copy Enter copy name select character with T and J keys Then press gt key to move to next character up to 8 characters BERG 2G Os cee No plate programmed PROGRAMMED Xp You must first ADD
5. step 0 1 s Time when dispensing at the Bottom Wash Vertical Position Plate parameters ASP HOR POS Aspiration Horizontal Position for flat bottom only 0 to 2 0 mm step 0 1 mm see also fig 8 at the end of 1 8 Aspiration Horizontal Position is applied for all Aspiration Bottom Wash and Bottom Aspiration sequences ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of S 1 8 0 1 is the highest needle position 15 0 is the lowest needle position Aspiration Vertical Position is applied for all Aspiration sequences B W VERT POS Bottom Wash Vertical Position 0 1 to 15 0mm step 0 1mm see also fig 8 at the end of 1 8 0 1 is the highest needle position 15 0 is the lowest needle position Bottom Wash Vertical Position is the height of the aspirating needle during the dispensing sequence of the bottom wash ASP DOWNW SPEED Aspiration Downward Speed 0 to 9 step 1 speed min 0 speed max 9 This speed is applied only at the first Aspiration sequence DISP UPW SPEED Dispensing Upward Speed 0 to 9 step 1 speed min 0 speed max 9 Dispensing Upward Speed is applied at the last Dispensing sequence BOT DOWNW SPEED Bottom Downward Speed 0 to 9 step 1 speed min 0 speed max 9 Speed of all downward movements for all bottom sequences wash and aspiration BOT UPWARD SPEED Bottom Upward Speed 0 to 9 step 1 speed min 0
6. 4 2 1 Cleaning the manifold The manifold is infectious Disinfect it prior to any further cleaning See disinfection procedure on next 4 2 2 Aspirate needle and chamber Remove the manifold Using a duster or a non scratching utensil thoroughly clean the outside of the dispense and aspirate needles Remove the silicon top seal of the manifold Using the big pin supplied in the maintenance kit clean the big aspirating needles Rinse out the aspirate chamber with deionized or distilled water Thoroughly clean the walls of the aspirating cavity Look through the aspirate needles to ensure that cleaning is adequate Put the silicon top seal back in its place Dispense needle and chamber Push the silicon cap from the chamber of the manifold using a stem or a screwdriver entered from the opposite end of the cylindrical chamber Using one of the small pin supplied in maintenance kit clean the small dispense needle Using the cylindrical brush supplied in maintenance kit thoroughly clean the small dispense chamber Thoroughly rinse dispense chamber with deionized water Ensure that all impurities are removed from the manifold Plug dispense chamber of the manifold with the silicon plug Place it as close as possible to the first needle however without blocking it Extra silicon caps are supplied in the maintenance kit Remount the manifold replace the tubing and reprime 63 4 2 Cleaning and disinfection continue
7. S Following message is displayed RAM INITIALIZATION YES Press YES to have the RAM reset 61 4 MAINTENANCE amp TROUBLESHOOTING 4 1Routine maintenance procedures 4 1 1 Daily maintenance Prior starting to wash It is recommended to pour an appropriate disinfection solution into the bottom of the WASTE bottle prior connecting it to the washer Thus the infected liquid coming from the washer and going into the WASTE bottle will be deactivated IMPORTANT Avoid bleach Fill the rinse bottle with deionized water Connect it to the washer Launch a Rinsing sequence and check that the pinch valve tube and manifold are not clogged Otherwise change the pinch valve tube and clean manifold see 4 2 1 hereafter When washing is terminated Rinse the washer Use only deionized water for rinsing Turn the washer off Tubes and manifold will be kept wet with water that will prevent them from clogging Empty the WASH bottle rinse it thoroughly with deionized water 4 1 2 Washer storage conditions The washer should never be stored with Wash solution inside dispensing tubes and manifold Otherwise the liquid pump the pinch valve tube and the manifold might be clamped if completely dry thus preventing any further dispensing of liquid In this case priming must be made with the help of a syringe filled with deionized water connected to the Rinse input of the washer 62 4 2 Cleaning and disinfection
8. YES or NO the aspiration needle is shifted close to the wall Aspiration Horizontal Po sition while going down to well bottom and aspirates once the aspiration time has elapsed the aspiration needle is going up to approx middle of well height and is shifted opposite end of wall no scratch on the bottom the needle goes down to bottom and a second aspiration sequence takes place ASP TIME Aspiration Time 0 1 to 9 9 s step 0 1 s Time during which the aspirating needle is kept at the well bottom and as pirates Plate parameters ASP HOR POS Aspiration Horizontal Position for flat bottom only 0 0 centered to 2 0 mm step 0 1 mm see also fig 8 at the end of 1 8 ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of S 1 8 0 1 is the highest needle position 15 0 is the lowest needle position ASP DOWNW SPEED Aspiration Downward Speed 0 to 9 step 1 speed min 0 speed max 9 18 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued D DISPENSING 5 The dispensing needle is placed over the well and dispenses a wash solu tion in the well When the dispensed volume is higher than well capacity the aspiration needle aspirates the overflow to prevent liquid spilling on next wells Programmed parameters Kit parameters VOLUME for one well 50 to 3000 ul step 50 ul the wel
9. a plate See also ERR 26 ERR 33 On 8 3 7 4 6 59 3 7PROGRAMMING continued 3 7 9 Plate Test Step by Step RUN The programmed kit can be used in a step by step mode i e manifold and plate carrier are stopped at each programmed positions Aspiration Horizontal Position Aspiration Vertical Position Overflow Position etc in order to check if these positions are correct If not correct the user must edit see 3 7 7 the tested kit or its assigned plate in order to modify position parameter s RUN KIT 08 1 i Use T or keys to select kit to be tested Press YES key to move to next programmed position Keep YES key pressed for a continuous motion preferably deactivate the beep The kit is processed on the whole plate 60 3 7 PROGRAMMING continued 3 7 10 RAM initialization Every programmed kit and programmed plate are saved on a RAM Random Access Memory The contents of this MEMORY can be erased by a RAM initialization thus deleting the whole set of kits and plates that have been programmed by the user Instrument software and DEMO kits and plates are not deleted as they are saved in an EPROM not affected by RAM initialization How to perform the RAM initialization Turn the unit ON while keeping those keys pressed A A A A A T Mu
10. minimize dripping In Main menu select CONFIGURATION available in firmware equal or more recent than 1 50 2Y for LP40 And 4 43 2Y for LP41 Pinch Valve Tube is leaking Change pinch Valve tube Spare supplied in Maintenance Kit Manifold connector with O rings is leaking Ensure O rings are correctly seated in manifold connector if still leaking change O rings Spare supplied in Maintenance Kit Contact your application specialist for advice 66 APPENDIX Revision December 1996 April 1997 September 1997 January 2000 November 2000 March 2003 April 2005 September 2005 Upgrade Description First official release of the user manual compatible for both LP40 and LP41 washers Implementation of the new motorised multiwasher selector Modified pages page 13 1 6 LP41 Hydraulic circuit page 38 3 6 2 Washer s configuration Implementation of the selection between the new multiwash selector MOTOR and the old one PINCH valve Page 39 modified accordingly and page 40 created Implementation of the new internal design with integrated blocking device of the manifold Modified pages page 28 2 installation Added Important Notice Modified page 4 New Adil logo e mail address ans website Modified Front page ADD V function Modified pages 46 48 49 50 Inversion of Aspiration horizontal position and Centering functions Modifi
11. the new plate has to be inserted Enter new method name Then same procedure as in parameter 3 7 4 5 ADD PLATE 52 3 7 PROGRAMMING continued 3 7 6 DELETE 3 7 6 1DELETE KIT KIT KIT Nr 24 YE KIT Nr 24 KIT KIT Nr 23 IN YE 3 ASPIRATIO ES ETHOD ASPIRATIO Select kit to be deleted Press YES key to confirm kit is dele ted or press ESC key to escape Select kit to which you want to de lete a method Select the method to be deleted Press YES key to confirm method is deleted or press ESC key to escape Note Deleting the method in a one method kit is equivalent to delete this kit 53 3 7 PROGRAMMING continued 3 7 6 DELETE continued 3 7 6 3 DELETE PLATE 3 7 6 4 ERROR in PLATE deletion Select plate to be deleted Press YES key to confirm plate is deleted or press ESC key to es cape The plate is still associated with one or several kits You must first associate an other plate to affected kits or deleted kits prior deleting this plate 3 7 PROGRAMMING continued 3 7 7 EDIT KIT Nr 24 4 KETS t Select k
12. 3 TEMPORARY KIT 51 Troubleshooting 65 U Unpacking 27 V Vertical Speed 48 VOLUME 19 W WASH 16 20 WASH ASP 16 Wash Aspiration 16 Wash Bottom Aspiration 16 WASH BOT ASP 16 2 8 CHANNEL 10 3 LIQUID INPUTS 10 8 CHANNEL 7 fax 056 260 270 befisher thermofisher com www be fishersci com fax 915159235 es fisher thermofisher com www es fishersci com fax 0388671168 fr commande thermofisher com www frfishersci com fax 02953 27 374 itfisher amp thermofisher com www it fishersci com fax 0204877070 nl info thermofisher com www fishersci nl fax 214253351 pt fisher thermofisher com www pt fishersci com fax 0566184141 info ch thermofisher com www ch fishersci com 69
13. 3 7 4 1 ADD KIT 43 3 7 4 8 ERRORS in KIT programming 44 3 7 4 8 ADD METHOD 45 3 7 4 4 ERRORS on METHOD programming 46 3 7 4 5 ADD PLATE 47 3 7 4 6 ERRORS on PLATE programming 50 3 7 5 INSERT 51 3 7 5 1 INSERT KIT 51 3 7 5 2 TEMPORARY KIT 51 3 7 5 3 INSERT METHOD 51 3 7 5 4 INSERT PLATE 52 3 7 6 DELETE 53 3 7 6 1 DELETE KIT 53 3 7 6 2 DELETE METHOD 53 3 7 6 8 DELETE PLATE 54 3 7 6 4 ERROR in PLATE deletion 54 3 7 7 EDIT 55 3 7 7 1 EDIT KIT 55 3 7 7 2 EDIT PLATE 57 3 7 8 Copy 58 3 7 8 1 COPY KIT 58 3 7 8 2 ERRORS in KIT copy 58 3 7 8 8 COPY PLATE 59 3 7 84 ERRORS in PLATE copy 59 3 7 9 PLATE TEST 60 3 7 10 RAM INITIALIZATION 61 4 MAINTENANCE amp TROUBLESHOOTING 62 4 1 ROUTINE MAINTENANCE PROCEDURES 62 4 1 1 DAILY MAINTENANCE 62 4 1 2 WASHER STORAGE CONDITIONS 62 4 2 CLEANING AND DISINFECTION 63 4 2 1 CLEANING THE MANIFOLD 63 4 2 2 DISINFECTION 64 4 3 TROUBLESHOOTING 65 4 3 1 HARDWARE AND SOFTWARE VERSION 65 RECOMMENDATIONS FOR OPTIMIZED WASHING CONDITIONS ON LP40 LP41 WASHERS 66 APPENDIX 67 REVISION UPGRADE DESCRIPTION 67 INDEX 68 IMPORTANT Please read carefully before using the PLATE WASHERS LP40 or LP41 This document is the copyright of Bio Rad and must not be copied or reproduced in any form without prior consent Bio Rad reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous program or product development This manual supersed
14. ATE mode 3W A on st strip 3W A on 2nd strip etc 3W A on last strip W on the whole plate W on the whole plate W on the whole plate A on the whole plate 16 1 8 Software parameters for Wash protocols continued 1 8 2 Methods contunued No OF CYCLES Number of Cycles n 1 to 9 step 1 number of elementary cycles that will be applied within the method SOAKING Soak time Omin Os to 59min Os step 15 in PLATE mode Omin Os to 9 9s step 0 1 s in STRIP mode Waiting time between two consecutive elementary cycle within one method MET INTER Method Interval Omin Os to 59min Os step 1 sec Waiting time between two consecutive methods 17 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions Parameters directly depending on the KIT or Wash protocol such as dispensing as pirating times liquids etc are called KIT Parameters Parameters depending on the microplate dimensions mainly POSITION and SPEED parameters are called PLATE parameters In the washer the elementary cycles are as follows A ASPIRATION ral ku ra The aspirating needle goes down and aspirates until reaching the well bot tom The needle is shifted close to wall Aspiration Horizontal Position for flat bottom well it remains centered for a curved bottom well Programmed parameters Kit parameters e CROSW ASP Crosswise aspiration CWA for flat bottom only
15. Default setting is MOTOR AL TYP MOTOR Use f and J keys to select PINCH T i YES ESC if the washer is equipped with a 4 pinch valve system old device A Return to the main menu Go to page 38 See 3 3 37 3 6 Configuration continued 3 6 2 Washer s configuration continued Continued from page 37 LP41 only Activation of the vacuum detection Use T and keys to set the vaccum sensing thershold Default valvue 20 points Use f and keys to activate YES or deactivate NO the vacuum detection Setting of the RS232 serial port for connection to PC BAUDS 9600 WAKE P Baud rate can be reduced in case of T 4 gt YES E transmission problem between PC and washer Use T and J keys to set the Baud rate Setting of Wake up function is ignored SEGUE TALR OUID Activation of the liquid detection in Wash and Waste bottles Use f and keys to activate YES or deactivate NO liquid level detector in Wash bottle then in Waste bottle Go to page 39 38 3 6 Configuration continued 3 6 2 Washer s configuration continued Continuation from page 38 GROUP Secured Stop 7 Y YE ESC FO SecurStop N VOI 7 Y gt Y
16. E Setting of dialogue interface parameters Use T and J keys to set language then gt key and and J keys to ac tivate Y or deactivate N the Beep Activation of a mini priming sequence before every wash protocol Use f and V keys to activate Y or deactivate N then gt key and 1 and to set the volume from 1 to 30ml Activation of the secured stop after every wash protocol to remove air from manifold Use T and keys to activate Y or deactivate N then gt key and f and to set the volume from 1 to 30ml Reboot all configurations s parameters Press Yes key to valid the reinitialisation Back to the main menu see 3 3 39 3 7 PROGRAMMING 3 7 1 Kit Structure KIT PROGRAMME Parameters depending on Kit reagents KIT 1 Kit NAME Plate Name MANIFOLD STRIP omitted METHOD MODE Crosswise Aspiration Aspiration Time Dispensed VOLUME OVERFLOW LIQUID FLOW compensation BOT WASH NUMBER BOTTOM Wash TIME BOT ASP NUMBER PLATE PROGRAMME Parameters depending on Plate dimensions PLATE 1 Name Bottom Form Aspiration Horizontal Position Centring Aspiration Vertical Position Bottom Aspiration Vertical P
17. I One or several dispensing needles must be cleaned Always ensure dispensing needles are clean Use metallic cleaning pin supplied in maintenance kit Owerflow position not set correctiy Adjust OVERFLOW position of the apsirating needle in order to aspirate the excess of dispensed VOLUME Kit parameter Often less volume in 1 strip than in the nextones Manifold dripping in rinsing tank when on standby before wash process La Maniflod dripping during Wash process High concentration of wetting agent in wash buffer Rubber stopper in manifold dispensing chamber not set correctly This might create an excessive air bibble inside the dispensing chamber of manifold leading to dripping Respect dilution factor Never use higher wetting agent concentration than required A dimension for Rubber Stopper mm Shape of rubber stopper Square Round Manifold channel 8 23 A lt 24 26 A x27 2 8 0 0 3 8 0 0 12 5 gt A 6 8 lt A lt 9 2 12 0 0 Rubber Stopper Jl Dispensing needles Manifold is dirty Clean manifold rinse and dry it completely Cleaning set supplied in Maintenance Kit Spray silicone grease onto dispensing needles Protect aspirating needles with adhesive tape Clean inside of dispensing needles with metallic pin supplied in maintenance Kit If still dripping Active MiniPrime and Secured Stop to compensate and
18. KIT 01 T 3 YES ESC RUN KIT75 1 af YES ESC LAST STRIP I 4 CONNECT THE WASH W9 ON A LES PUMP PRIMING RUN KIT 75 Strip 8 RUN KIT 75 WASH t ASP RUN KIT 75 T 4 Use the T and keys to select kit Use the 1 and keys to select last strip to be processed For LP40 only This message is dis played when the requested wash buffer is not connected Once you have connected the correct WASH bottle press YES and the pump will start to prime in order to ope rate with the correct WASH liquid For LP41 See ERR 21 on next page Summary of the selected item e Kit name e Number of strip Once the kit is launched the ongoing method is displayed on lower display li ne Press ESC to stop running the kit The kit is terminated Display is back to previous kit selection See 8 3 4 1 31 3 3 Select one KIT continued ERR 21 with LP41 RUN KIT 01 LAST STRIP 1 i ERR 21 WASH W9 NOT CONNECTE YES Error 21 indicates that Wash buffer W9 requested in KIT 75 is not connected Connect Wash buffer W9 at input 1 2 or 3 Then enter washer CONFIGURATION to modify the input assignment accordingly see 3 6 2 32 3 4 WASH interruption or Power Failure 3 4 1 WASH interruption When the ESC key is pressed whereas a kit is running the following message is di
19. Microplate Washer LP40 LP41 User manual User Manual code 40B030e Revision September 2005 English 3 rue Denis Papin 67300 Schiltigheim France tel 33 3 88 19 70 50 fax 33 3 88 62 52 98 TABLE OF CONTENTS ABOUT THIS MANUAL IMPORTANT NOTICE SAFETY INFORMATION SAFETY SYMBOLS 1 FEATURES AND SPECIFICATIONS 1 1 LP40 BASIC FEATURES 1 2 LP40 OUTSIDE DIMENSIONS mm 1 3 LP40 HYDRAULIC CIRCUIT 1 4 LP41 BASIC FEATURES 1 5 LP41 OUTSIDE DIMENSIONS mm 1 6 LP41 HYDRAULIC CIRCUIT 1 7 TECHNICAL FEATURES 1 7 1 ELECTRICAL DATA 1 7 2 PHYSICAL DATA 1 7 3 HARDWARE SPECIFICATIONS 1 7 4 SOFTWARE SPECIFICATIONS 1 8 SOFTWARE PARAMETERS FOR WASH PROTOCOLS 1 8 1 WASH PROTOCOL DEFINITION 1 8 2 METHODS 1 8 3 PARAMETER S DEFINITIONS 2 INSTALLATION 2 1 UNPACKING 3 OPERATING INSTRUCTIONS 3 1 RUN MODE 3 1 1 How To USE THE KEYBOARD 3 2 MICROPLATE LOADING 3 3 SELECT ONE KIT 3 4 WASH INTERRUPTION OR POWER FAILURE 3 4 1 WASH INTERRUPTION 3 4 2 POWER FAILURE 3 5 RINSE 3 5 1 RINSE WITH LP40 3 5 8 RINSE WITHLP41 3 5 4 X AUTOMATIC RINSING WHIT LP41 3 6 CONFIGURATION 3 6 1 MAIN MENU 3 6 2 o WASHER S CONFIGURATION 3 7 PROGRAMMING 10 11 12 13 13 13 13 14 15 15 16 18 27 27 29 29 29 30 31 33 33 33 34 34 35 35 36 36 37 40 3 7 1 KIT STRUCTURE 40 3 7 2 AccEss To PROGRAMMING MODE 41 3 7 3 PROGRAMMING SYNOPSIS 42 3 7 4 ADD 43
20. NGINEER In some operating modes e g dispensing fluids or purging the system particularly under automatic control it is possible to overfill the wells of a microplate This can result in spillage of liquids and or contamination with hazardous substances Care should be taken to ensure that overfilling does not occur If environmentally hazardous fluid is spilled the instrument must first be isolated from the electrical supply and physically isolated from the working environment by placing it in a fume cupboard or similar location Contact the nearest service centre for assistance advising them of the health hazards associated with the spilled materials OPERATING THIS EQUIPMENT IN WAYS OTHER THAN DETAILED IN THIS MANUAL MAY IMPAIR THE PROTECTION PROVIDED BY THE INSTRUMENT DO NOT operate this equipment with the covers removed potentially dangerous voltages are contained within DO NOT operate this equipment with the safety earth ground disconnected CAUTION DO NOT poke fingers pencils or other implements into plate carrier entry slot BE PREPARED for unexpected movement of the plate carrier and noise from the Washer pump when the Washer is controlled by external computer ENSURE that the main power cable is correctly wired Colour codes are as follows Europe United States BROWN LIVE BLACK LIVE BLUE NEUTRAL WHITE NEUTRAL GREEN YELLOW EARTH Ground GREEN GROUND ENSURE that all connections are tight and all blocking elem
21. OO 00000 Electro valve Liquid Pump Blue Vaccum Pump Hydrophobic Filter Fig 3 1 4 LP41 Basic features Aerosol Protection Cover 2x20 characters LCD Display and 5 soft Keys RS232 serial port for connection to PC 4 liquid inputs Manifold 8 2 8 3 8 Channel or 12 8 Mode 12 Mode Plate Carrier 2 12 Channel Removable Removable Autoclavable at 120 C 250 F Fig 4 1 5 LP41 Outside dimensions mm gt 174 OOO OO ER 280 325 _ _____ 277 5 4 525 5 Fig 5 193 gg 123 ERE 11 1 6 LP41 Hydraulic circuit Liquid Level Detection in Bottle Vacuum Sensing 2 8 Channel Manifold 2 Vaccum Pumps WASH or RINSE Solution Inputs Yellow Hydrophobic Filter WASTE bottle Fig 6 Depending on options 12 1 7 Technical features 1 7 1 Electrical Data Voltage Consumption Fuses Power Cord 1 7 2 Physical data 240 100 VAC 50 60 Hz 100 VA max 2 5 AT 2 fuses 5x20 mm on CEI socket Dimensions including bottles Width
22. PLATE PRG INSERT See 3 7 5 INSERT KIT INSERT METHOD INSERT PLATE PRG DELETE See 3 7 6 DELETE KIT DELETE METHOD DELETE PLATE PRG EDIT See 3 7 7 EDIT KIT EDIT PLATE PRG COPY See 3 7 8 COPY KIT COPY PLATE Fig 16 PRG PLATE TEST RUN KIT 75 See 3 7 9 42 3 7 PROGRAMMING continued 3 7 4 ADD 3 7 4 1 ADD KIT The kit is added at the end of the existing kit list Use 1 and keys to scroll the available menu Press YES key to initiate the displayed selection Press ESC key to escape and go back to former prompt Enter the kit name select character with T and keys then press gt key to move to next character up to 15 characters n Select the plate to be associated to kit MANIFOLD 8 Select manifold type 7 YES ESC LP40 8 or 12 channels LP41 8 2 8 3 8 or 12 2 12 channels Omit strip place cursor on strip with gt key then press T or key to have strip omitted Xx In RUN mode the omitted strips will never be processed STREP ST 8 910 X 12 ES ESC ETHOD WASH ASP ES ESC Select the first method of kit See method parameter selection on 3 7 4 3 Answer NO in order to add another method
23. RORS in KIT programming ERRORS in METHOD programming ERRORS on PLATE programming F FLOW Flow rate compensation H Hardware specifications Hardware version HORIZONTAL SPEED j IN INSERT INSERT METHOD INSERT PLATE INSERT KIT K Keyboard KIT KIT Parameter Kit parameters Kit Structure 16 44 62 53 54 64 19 19 19 68 L LIQUID LP40 Basic features LP40 Outside dimensions LP41 Basic features LP41 Outside dimensions M Main Parameters maintenance Manifold MEMORY MET INTER method Method Interval Methods Microplate loading Mini Priming 0 Omit Strip Out OVERFLOW Overflow Position P Physical data Plate Agitation Plate Carrier Plate Mode PLATE parameter Please RINSE Prime PROGRAMMING in Advanced Mode Programming Synopsis Pump Priming Belgique Belgi Espa a 5 2 ds France Fisher Scientific Italia Part of Thermo Fisher Scientific Netherlands Portugal Suisse Switzerland 15 t l 19 10 11 056 260 260 tfno91 515 92 34 t l tel tel tel t l 03 88 67 53 20 02 953 28 258 020 487 70 00 214253350 0566184111 R Routine maintenance 62 RS232 serial port for connection to PC 10 5 Secured Stop 39 safety information 5 SHAKE TIME 25 SHAKING AMPLITUDE 25 SHAKING SPEED 25 Soak time 17 SOAKING 17 Software parameters for Wash protocols 15 Software specifications 14 Software version 65 Strip Mode 16 T Technical features 1
24. YES key to initiate the displayed selection Press ESC key to escape and go back to former prompt KIT No 23 m e Select kit to which you want to add a me thod with the 1 and keys Set Method interval between last exis ting method and added method with f or L and gt keys from 0 min 0 s up to 59 min O s EU Se ee RE o es Select the method to be added Set kit parameters associated to selected method See table next page and kit parameter definition 1 8 3 Press V key to show the modification at OVERETON 3 0mm the real time To go out from this mode M V YES ESC just press V key again This mode can be kept during all the LIQUID WASH W9 steps of modifications T l YES ESC FLOW 3 1 l YES ESC Only the first cycle is played n times here twice for a two cycle method such as Wash Asp nW A 45 3 7 PROGRAMMING continued 3 7 4 ADD continued 3 7 4 3 ADD METHOD continued Summary of kit parameters associated to method METHOD n z o 05 9 als o Z gt Z lt n 0 Nj IT lt 0 lt Z EM oot ea gt B 0 AR o a a B gt gt 0 lt lo 2 o O
25. d 4 2 2 Disinfection The disinfection program applies to internal surfaces of tubing connectors manifold and manifold needles ECT DISINFECTION IN YES OUT Er DLS EN Es Use disinfection solution approved by your labora TESE tory Connect the tube s on rinse bottle cap Fill the RINSE bottle with the disinfection solution Ensure the plate carrier is not removed then press YES PUMP PRIMING DISINFECTION Pumps are stopped whereas dispensing circuits are 0 H30mn00s E filled with disinfection solution A countdown of 30 min is started after which you are prompted to perform a RINSE operation Remove disinfection solution and fill RINSE bottle with deionized water DiH2O Then press YES Then prior launching a Wash protocol assign the correct Wash solution to the wash input s in the CONFIGURATION menu or by priming the circuit with the PRIMING RINSING menu PUMP PRIMING A Return to the main IMPORTANT Avoid bleach in disinfecting the washer as a thorough rinsing of the tubes will not prevent traces of bleach from remain inside the tubing and manifold This can be enough to corrupt the reagents of next wash Disinfect the washer when it has not been used for more than one day When used daily disinfect it at least once a week Disinfect the washer prior to any servicing or handling action shipment or change of s
26. e programming 50 3 7 PROGRAMMING continued 3 7 5INSERT 3 7 5 1INSERT KIT The new kit is inserted before another kit Select kit before which you want to insert new kit Enter new kit name T M e Eee D Then same procedure as in 8 3 7 4 1 ADD KIT 3 7 5 2TEMPORARY KIT RUN TEMPORARY KIT T 4 YES ESC Power failure or erroneous OFF switching during KIT insertion will cause the kit to be lost unless the first method has been completely programmed In this case the com pletely programmed methods can be found in a kit named TEMPORARY KIT This kit is always inserted at the first place of the kit list It is then possible to COPY this Temporary Kit and write its correct name then continue with programming of next methods and kit repetition parameters Once saved under its correct name it is highly recommended to DELETE the TEM PORARY KIT 3 7 5 3 INSERT METHOD The new method is inserted before another method Select kit where a method has to be inserted Select method before which the new method has to be inserted YES METHOD DISPENSING Enter the new method name Then same procedure as in 3 7 4 3 ADD METHOD 7 4 YES 51 3 7 PROGRAMMING continued 3 7 4 INSERT continued 3 7 5 4 INSERT PLATE The new kit is inserted before another plate Select the plate before which
27. ed page 48 ADD reset config function Modified page 40 Manual User converted in Word format Adil instruments logo remplecement by Bio Rad s one Addition of NOTA on page 3 67 INDEX Symbols A Access to PROGRAMMING ADD KIT ADD METHOD ADD PLATE Aerosol Protection Cover AGITATION Agitation Amplitude Agitation speed Agitation Time ASP DOWNW SPEED ASP HOR POS ASP TIME ASP VERT POS ASPIRATION Aspiration Downward Speed Aspiration Horizontal Position Aspiration time Aspiration Vertical Position Autoclavage B BEEP B W VERT POS B WASH B ASP BOT ASP NUMBER BOT DOWNW SPEED BOT SHAPE BOT UPWARD SPEED BOT VERT POS BOT WASH NUMBER BOT WASH ASP Bottom Aspiration Vertical Position BOTTOM ASP Bottom Aspiration Number Bottom Downward Speed BOTTOM TIME Bottom Upward Speed Bottom Wash Number Bottom Wash Time Bottom Wash Vertical Position BOTTOM WASH C CENTERING Cleaning the manifold Compensation Contents COPY KIT Crosswise aspiration CROSW ASP cut off device CWA 16 16 16 23 16 47 19 2 58 18 18 18 D Daily maintenance DELETE DELETE METHOD DELETE PLATE DELETE KIT Disinfection DISP UPW SPEED DISPENSING Dispensing Upward Speed E EDIT METHOD END OF EDIT EDIT KIT Electric Shock hazard Electrical Data ERR 21 ERR 24 ERR 25 ERR 26 ERR 27 ERR 28 ERR 29 ERR 32 ERR 33 ERROR on PLATE deletion ER
28. eedles and clamp the electrovalve tube 35 3 6 Configuration 3 6 1 Main menu RUN See 3 1Run Mode PRIME RINSE See 3 5 RINSE DISINFECTION See 4 2 2 DISINFECTION CONFIGURATION See 3 6 2 Washer s configuration SERVICE VER See 4 3 1 Hardware and software version 36 3 6 Configuration continued 3 6 2 Washer s configuration Enter CONFIGURATION menu ECT CONFIGURATION IN YES OUT ROUP MANIFOLD A 4 YES ESC Selection of Manifold MANIFOLD A 2 7 4 YES ESC Use 1 and keys to set the type according to installed manifold x channel manifold LP41 only Assignment of Wash amp Rinse inputs on the rear panel Use f and 1 keys to select the input number P She eis ipm Use T and J keys to assign wash W1 to W9 or rinse solution to the selected input Note input 4 is mandatory assigned to Rinse GROUP RINSE T i Setting of countdown prior to launching automatic rinsing is t s x E nU Ge Use f and keys to set countdown from 0 to 240 minutes Automatic Rinse is deactivated when Rinse Time is set at O Selection of the multiwash selector type on rear panel
29. ents foams have been removed before switching on the Washer If there is any doubt or concern about the safety of the instrument contact the nearest approved service center SAFETY SYMBOLS The following alarm and information symbols can be found in several places in the washer Only a qualified personal aware of the security procedures is allowed to repair this instrument Please read this manual carefully before using this instrument pO eq v9 2 Direct current Alternating current Direct and alternating current Earth ground terminal Protective earth ground terminal On Off Caution see enclosed documents 1 FEATURES AND SPECIFICATIONS 1 1 LP40 Basic features Aerosol Protection Cover 2x20 characters LCD Display and 5 soft Keys Manifold 8 Channel or 12 Channel Removable 8 Mode 12 Mode Plate Carrier Removable Autoclavable at 120 C 250 F Fig 1 1 2 LP40 Outside dimensions mm pas 277 5 193 amd 174 Sy 5 P gt 6 525 5 d d OOO OO 325 Fig 2 1 3 LP40 Hydraulic circuit WASH or RINSE Solution Input 8 channel or 12 channel Manifold O
30. ertical Position dispensing at this position during a Bottom Wash Time Followed by a downward aspirating movement down to Aspiration Verti cal Position Aspiration at Aspiration Vertical Position during Aspiration Time Dispensing up to Overflow Position In case of a crosswise aspiration for flat bottom only each aspiration sequence close to the wall is immediately followed by another aspiration sequence to the opposite end of the wall as described on page 18 Programmed parameters Kit parameters e CROSW ASP Crosswise aspiration for flat bottom only YES or NO See details on page 18 e ASP TIME Aspiration time 0 1 to 9 9 s step 0 1 s See details on page 18 e VOLUME for one well 50 to 3000 ul step 50 ul the well volume is approx 370 uL Volume is applied at the last Dispensing sequence e OVERFLOW 1 0 to 12 9 mm step 0 1 mm 1 0 is the highest needle position 12 9 is the lowest needle position for the overflow Overflow is applied at the last Dispensing sequence See details on page 19 e LIQUID Wash W1 to Wash W9 See details on page 19 FLOW flow rate compensation 5 to 5 step 1 See details on page 19 21 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued Bottom Washing continued BOT WASH NUMBER Bottom Wash Number 10r2 Number of Bottom Wash Occurence BOTTOM TIME Bottom Wash Time 0 1 to 9 9 s
31. es all previous editions NOTA The LP40 41 microplate washers can be integrated into a system together with other instruments in order to make biologic medical assays About this manual This manual has been written for the laboratory technician and describes how to use the LP40 and LP41 plate washers This manual is designed to give you the necessary information you need to install the LP40 or the LP41 operate with the LP40 or the LP41 to suit your particular needs perform the LP40 or the LP41 basic maintenance procedures including disinfection This manual also describes all the features and specifications of the LP40 and LP41 hardware and configuration software Important Notice Before installing this unit please read carefully the installation instructions This instrument can only be used to wash 96 well microplates with 8 12 or 2 8 channel manifolds or 384 well micro plates with 1 6 channel manifold This washer is intended for clinical diagnostic and research laboratory use and must be operated only by specialized personnel aware of the potential risks connected to the chemical and bacteriological agents normally used with this unit The Aerosol Protection Cover must always be closed when the instrument is operated to reduce the risk of contamination from ambient air Since the unit is not completely sealed this risk is not completely eliminated and the operator should dress with appropriate safe
32. haken horizontally during the Agitation Time with an Agitation Amplitude and an Agitation Speed Programmed parameters Kit parameters e SHAKE TIME Agitation Time gt 0 1 to 59 9 s step 0 1 s Plate parameters e SHAKING AMPLITUDE Agitation Amplitude 0 to 9 step 1 e SHAKING SPEED Agitation Speed 0 to 9 step 1 25 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued The Identification and range of parameters related to the location of the needle in relation to the microplate well Graduations in this sketch are only for better understanding Set these parameters in real conditions with real plate Well dimension may va ry from one plate type to another one Aspiration Vertical Position Bottom Aspiration Vertical Position Bottom Wash Vertical Position 0 1to 15 0 mm Plate parameters Fig 8 Dispensing needle 0 HOME POSITION Overflow Position 1 0 to 12 9 mm Kit parameter Microplate well Aspirating needle Aspiration Horizontal Position 0 1 to 2 0 mm Plate parameter mm m 10 11 12 13 14 15 26 2 INSTALLATION 2 1 Unpacking Fig 9 Carefully unpack accessories and washer IMPORTANT Do not lift up the washer by pulling on the foam frames First remove accessories at the top and beside the washer Then hold the washer not the foams and
33. ion time 0 1 to 9 9 s step 0 1 s See details on page 18 VOLUME for one well 50 to 3000 ul step 50 ul the well volume is approx 370 ul OVERFLOW 1 0 to 12 9 mm step 0 1 mm 1 0 is the highest needle position 12 9 is the lowest needle position for the overflow See details on page 19 LIQUID Wash W1 to Wash W9 See details on page 19 FLOW flow rate compensation 5 to 5 step 1 See details on page 19 Plate parameters ASP HOR POS Aspiration Horizontal Position for flat bottom only 0 0 to 2 0 mm step 0 1 mm see also Fig 8 at the end of 81 8 ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of 1 8 0 1 is the highest needle position 15 0 is the lowest needle position e ASP DOWNW SPEED Aspiration Downward Speed 0 to 9 step 1 speed min 0 speed max 9 DISP UPW SPEED Dispensing Upward Speed 0 to 9 step 1 speed min 0 speed max 9 20 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued w BOTTOM WASH Bottom Washing The bottom of the well is thoroughly washed followed by a normal Wash W sequence Following sequences are applied Downward aspirating movement of the needle into the well down to Aspi ration Vertical Position Aspiration at Aspiration Vertical Position during Aspiration Time One or two upward dispensing movements up to Bottom Wash V
34. it to be edited Select MAIN PARAMETERS or ME THOD with 1 and J keys EDIT MAIN PARA T Y YE Go to page 56 E E Edit main parameters Every displayed parameter can be changed by using T or keys Example the plate associated to this kit can be changed KIT 7 INTE 4 Press YES to validate the main parame ters modification Press 1 or keys until END of EDIT message Press YES to quit EDIT mode Press 1 or keys to go back to Main Parameters or Method A Return to the main menu see 3 3 55 3 7 PROGRAMMING continued 3 7 7 EDIT continued 3 7 7 1 EDIT KIT continued Continued from page 55 ES Select method to be edited Then every parameter of the method can be modified by using 1 or 4 keys Note that the Liquid Wash Wx cannot be modified Press YES to validate method modifica tion Press 1 or keys until END OF EDIT message Press YES to quit EDIT mode Press 1 or J keys to go back to Main Parameters or Method A Back to main menu see 3 3 C
35. ite The final RINSING sequence must be performed in all cases after the disinfection procedure has been started even for demonstration purpose The software uses this final rinsing sequence to set the Rinse liquid at all 4 inputs The automatic rinsing will not be possible ERR 18 if rinsing is not done after disinfection 64 4 3 Troubleshooting 4 3 1 Hardware and software version 1 and J keys are deactivated on this prompt Hardware version Software version of the washer Default User Data version Kit list and Plate list programmed in the factory and saved on EPROM Default user data are not deleted by a RAM initialisation A Back to main menu see 3 3 65 Recommendations for optimized Washing conditions on LP40 LP41 washers Bad Washing condition Factor generating risks How to restore good washing conditions Comments Inadequate aspiration Adjust Aspiration Parameters Too much residual volume in the wells ied One neddle forgets temporarily to aspirate Aspiration Vertical Position not set correctly ASPiration VERTical POSition must be touching bottom of well to avoid aspiring needles blocked by foam Aspiration Horizontal Position not set correctly ASPiration HORizontal POSition must be as possible to well s wall Use CROSswise ASPiration Com
36. ith at least 5 RINSE operations see RINSE mode 3 5 The washer is ready for use 28 3 OPERATING INSTRUCTIONS 3 1 Run Mode 3 1 1 How to use the Keyboard VERSION 4 xx 2Y LP 41 A A A A A A A A Press the OUT key to set or remo ve microplate plate carrier moves out and is locked for 10 seconds to allow the microplate to be placed in carrier Press 1 or keys to scroll the main menu See 3 6 1 Main menu on page 36 Press the YES key to enter the selected mode Press IN key to move plate carrier in RUN KIT 75 WA SH ASP The function of the key is displayed on the Screen Depending on the displayed menu Some keys are activated some others have no function and are there fore deactivated 29 3 2 Microplate loading The plate carrier supports both 8 and 12 modes for processing 96 well microplates Place the microplate on the plate carrier according to the mode selected Washer configurated in 8 mode with 8 2 8 3 8 Channel Manifold One strip is made of 8 wells numbered A to H Number of strips 12 numbered 1 to 12 Fig 13 Washer configurated in 12 mode with 12 2 12 Channel Manifold One strip is made of 12 wells numbered 1 to 12 Number of strips 8 numbered A to H 30 3 3 Select one KIT SELECT RUN i 5 IN YES OUT RUN
37. l the availa ble menu Press YES key to initiate the displayed selection Press ESC key to escape and go back to former prompt Enter plate name select character with M and keys then press the key to move to next character up to 8 cha racters Indicate well bottom shape FLAT or CURVED Press V key to show the modification at the real time To go out from this mode just press V key again This mode can be kept during all the steps of modifications Centring of the uplate wells relatively to manifold needle in the In Out direction 2 0 mm IN to 2 0 mm OUT step 0 1 mm Important parameter when a crosswise aspiration must be made close to the wall of wells Aspiration Horizontal Position for FLAT bottom only See definition on para 1 8 3 Aspiration Vertical Position See definition on para 1 8 3 47 3 7 PROGRAMMING continued 3 7 4ADD continued 3 7 4 5 ADD PLATE continued Continued from page 47 ERT POS 11 8mm V YES E E HORIZONTAL SP 7 Y V EED 6 YES ESC ERTICAL SPEE ASP DOWNW SPEED 6 7 4 V YES ESC p Go to page 49 Bottom aspiration Vertical Position See definition on para 1 8 3 Bottom Wash Vertical Position See definition on para 1 8 3 Horizontal Speed In Out plate carrier speed during non wo
38. l volume is approx 370 ul OVERFLOW 1 0 to 12 9 mm step 0 1 mm see also fig 8 at the end of 1 8 This parameter sets the height of the aspirating needle and thus the height of the liquid in the well 1 0 is the highest needle position 12 9 is the lowest needle position for the overflow It is recommended to dispense a volume slightly higher than wished and have an overflow phase in order to obtain an equal dispensing of volume in each well The slight flow rate variations among dispensing needles are compensated by the overflow phase LIQUID Wash W1 to Wash W9 When launching a kit in the RUN mode the display will remind you to connect correct Wash bottle prior starting FLOW Flow rate compensation 5 to 5 step 1 The flow rate of the dispensing pump is adapted to the operating manifold However the flow rate can be slightly modified with this parameter lower flow rate whith negative value higher flow rate with positive value Plate parameters DISP UPW SPEED Dispensing Upward Speed 0 to 9 step 1 speed min 0 speed max 9 19 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued W WASH Du im Aspiration sequence followed by a Dispensing sequence Programmed parameters Kit parameters e CROSW ASP Crosswise aspiration for flat bottom only YES or NO See details on page 18 ASP TIME Aspirat
39. lift up Remove the foams remove plastic bag and place washer on a stable horizontal surface Slightly open aerosol cover and remove thin piece of foam pull it down Fig 10 27 2 1 Unpacking continued e In washer working area Fig 11 12 amp 13 Ea Remove adhesive tape blocking aerosol cover fel Remove adhesive tape blocking plate carrier to Ly bottom plate fig 11 e Pull and push plate carrier to check that it moves freely e Manifold Remove the tie blocking the manifold on the left side fig 12 push the tie to the left Remove Fig 11 the tie from the vertical shaft Turn blocking device as shown on fig 12 and ensure to not hinder the manifold Up Down motion e On rear panel Connect tubes on rear panel match colours e Vacuum pump protection Fig 12 Cut yellow tube at halfway between WASTE bottle and washer and place hydrophobic filter supplied in maintenace kit e Connect power cord Fuse installed 2 5 A Slow Blow Plug Power Cord to mains socket washer is equipped with universal input switch mode power supply 85 VAC to 264 VAC 47 Hz to 440 Hz e Turn the washer ON ON OFF switch on rear panel Whereas plate carrier and manifold are moving to their home position following message appears on display After initialization V ERSION 2 xxx 2Y LP41 A A A A A A Fill one RINSE bottle with demineralized water and prime the hydraulic circuit w
40. osition Bottom Wash Vertical Position Horizontal Speed Vertical Speed Aspiration Speed In Dispensing Speed Out Bottom Wash Speed In Bottom Wash Speed Out SHAKE TIME No OF CYCLES Agitation Amplitude SOAK time Agitation Speed END OF KIT NO MET hod INTER val Fig 15 METHOD MODE Crosswise Aspiration Etc As seen on 1 8 3 Parameter s definitions KIT and DA PLATE parameters directly depending on KIT such NR OF KIT as dispensing aspirating times liquids etc are cal KIT INTER val led KIT parameters Parameters depending on microplate dimensions mainly POSITION and SPEED parameters are cal led PLATE parameters At programming each kit is mandatory linked to one plate with the Plate number parameter Fig 15 This implies that the PLATE must be already existing or must be programmed prior the associated KIT is programmed Up to 10 PLATES can be programmed Up to 75 KITS can be programmed 40 3 7 PROGRAMMING continued 3 7 2 Access to PROGRAMMING mode SELECT RUN t IN A A A A Enter the programming mode press simultaneously the IN and OUT keys YES 2 td YES 41 3 7 PROGRAMMING continued 3 7 3 Programming Synopsis Programming Mode PRG ADD See 3 7 4 ADD METHOD ADD
41. parameter sets programmable Accepts flat or curved bottom microplates Vertical and horizontal speeds vertical and horizontal positions of the aspirating needle in relation with the wells are programmable Priming sequence of the hydraulic system at change of Wash solution Disinfection program of the hydraulic circuit User programming can be inhibited 14 1 8 Software parameters for Wash protocols This section describes the Wash protocols their methods and their software parameters It is useful to be read prior starting to programme new wash protocols or new kits 1 8 1 WASH protocol definition A WASH protocol also called a KIT is made of a succession of wash Methods There are 10 different wash methods available and every method is programmed with its own batch of specific parameters Also the Wash protocol is associated to a batch of plate parameters Positions that are set in respect to plate geometry flat U or V shaped well as well as Speeds needed for the wash process Plate 10 Positions and speeds Wash protocol 75 or Kit 75 Plate 1 Positions and speeds Method Method name Method 1 Single cycle method Wash Aspiration Dispensing Method 2 Bottom Washing Bottom aspiration Plate Agitation Method 3 Two cycle method Wash Aspiration Wash Bottom Aspiration Bottom Wash Aspiration Bottom Washing Bottom Aspiration 15 1 8 Software parameters for Wash p
42. pensable possible horizontal offset with CENTERING parameter Home Position Plate parameter Plate parameter Aspiration Vertical Position Kit parameter Plate parameter UIT 3 Aspiration Downward Speed not set correctly Reduce ASPiration DOWNward SPEED E Aspiration Plate parameter Horizontal position High concentration of wetting agent in wash buffer Respect dilution factor Never use higer wetting agent Hydrophobic needle might be blocked by foam concentration than required Filter Waste bottle is full Check waste bottle ensure no foam goes on the yellow tube Si Empty Waste Bottle prior starting to wash U Hydrophobic Filter is clogged Change Hydrophobic Filter Possibly install a trap vial Waste Bottle fag e Trap Vial Contact your application specialist for advice Inadequate dispensing Adjust Dispensing Parameters Too weak n Too strong Flow Rate is not set correctly Adjust FLOW rate compensation between 5 weak and 5 strong Kit parameter Not enough quantity Volume is not set correctly Adjust VOLUME between 50 to 3000 uL by step of 50 uL Always dispense slightly more than the awaited volume in the well and aspirate the volume in excess by setting the OVERFLOW position see below Kit parameter Not the same volume in every well ALL
43. rk movements movement to home position return movement from last to first strip etc 0 to 9 step 1 speed min 0 speed max 9 Vertical speed Up Down manifold speed during non work movements movement to home position etc 0 to 9 step 1 speed min 0 speed max 9 Aspiration Downward Speed See definition on para 1 8 3 48 3 7 PROGRAMMING continued 3 7 4ADD continued 3 7 4 5 ADD PLATE continued Continued from page 48 BOT UPWARD SPE ir Al V YE ED 6 S ESC T G AMPLIT 7 Y VY T UDE 2 ES ESC H Dispensing Upward Speed See definition on para 1 8 3 Bottom Downward Speed See definition on para 1 8 3 Bottom Upward Speed See definition on para 1 8 3 Shaking Amplitude See definition on para1 8 3 Shaking Speed See definition on para1 8 3 49 3 7 PROGRAMMING continued 3 7 4ADD continued 3 7 4 6 ERRORS on PLATE programming ERR 33 PL IMPOSSIBLE 10 plates are already pro grammed You must first dele te an existing plate prior to programming a new one You have entered no name or the plate name you have ente red is already existing You are prompted to enter a new name to continue plat
44. rotocols continued 1 8 2 Methods A method is a completely defined module made of one or two elementary cycles Every kit is made of a succession of methods In a method elementary cycles can be repeated n times n 1 to 9 For a method using two elementary cycles only the first cycle is applied n times When repeated several times the waiting time between elementary cycles is called soaking time The waiting time between two methods is called Method Interval Whatever the selected mode STRIP or PLATE see definition below a method is al ways completely finished on the whole plate prior to starting the next method Available methods Method Abbreviated name Method name on LCD display Single cycle method nw nA nD nw na nAg WASH Wash ASPIRATION Aspiration DISPENSING Dispensing BOTTOM WASH Bottom Washing BOTTOM ASP Bottom aspiration AGITATION Plate Agitation Two cycle method nW A WASH ASP Wash Aspiration ta WASH BOT ASP Wash Bottom Aspiration nw A BOT WASH ASP Bottom Wash Aspiration nw ta B WASH B ASP Bottom Washing Bottom Aspiration Kit parameters associated to method e MODE STRIP or PLATE Example Method 3W A STRIP mode the total method is applied on the strip prior processing the next strip STRIP mode PLATE mode v3 the elementary cycle is executed successively on all strips prior applying the next elementary cycle PL
45. should never be switched off with the WASH solution inside the tubing and manifold Solid crystals from the dried WASH solution could clog the dispense needles and clamp the electrovalve tube 34 3 5 RINSE continued 3 5 3 Rinse with LP41 E RINSE ES OUT Way is mandatory A on LP41 Press YES to continue Select washer input according to the rinsing or priming that you want to do INPUT 4 SO ba 3 RINSE Indication of the liquid connected to the 1 Y TEE selected input If you change the liquid use T or keys to modify input assignment same as in the washer configuration on page 37 Note Input 4 is mandatory RINSE PRIMING RINSING Back to the main menu See S 3 3 3 5 4 Automatic Rinsing whit LP41 PRIMING RINSING ESC One automatic rinsing sequence will take place as soon as the time set in RINSE PARAM see 3 6 2 Washer s configuration has elapsed after the last wash Note When the washer won t be used for several days it is recommended to connect all channels all inputs to rinse bottle and rinse every input as described above in order to clean the pinch valve tube of every input See also 4 1 2 Storage condition IMPORTANT The washer should never be switched off with WASH solution inside tubing and ma nifold Solid crystals from the dried WASH solution could clog the dispense n
46. speed max 9 Speed of all upward movements for all bottom sequences wash and aspi ration 22 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued a Bottom aspiration BOTTOM ASP The following sequence are applied Downward aspirating movement of the needle into the well down to Aspi Th ration Vertical Position Aspiration at Aspiration Vertical Position during Aspiration Time One or two upward aspirating movements up to Bottom Aspiration Posi tion followed by a downward aspirating movement down to Aspiration Ver tical Position Aspiration at Aspiration Vertical Position during Aspiration Time In the case of crosswise aspiration for flat bottom only every aspiration sequence close to the wall is immediately followed by another aspiration sequence to the opposite end of the wall as described on page 18 Programmed parameters Kit parameters e CROSW ASP Crosswise aspiration for flat bottom only YES or NO See details on page 18 ASP TIME Aspiration time 0 1 to 9 9 s step 0 1 s See details on page 18 BOT ASP NUMBER Bottom Aspiration Number 1 or 2 Number of Bottom Aspiration Occurence Plate parameters ASP HOR POS Aspiration Horizontal Position For flat bottom only 0 0 to 2 0 mm step 0 1 mm see also fig 8 at the end of 1 8 Aspiration Horizontal Position is applied for all Aspiration Bottom Wash and Bottom Aspiration seq
47. splayed ST STOP THE TE Oh 3min 17s The elapsed time since the kit has been stopped is displayed Press the RES key to resume the kit at the point where it has been stopped or press the ESC key to go back to main menu RE RUN KIT 75 WASH ASP KIT 75 Y RUN T A Back to the main menu see 3 3 3 4 2 POWER Failure When a power failure occurs whereas a kit is running the following message will be displayed when power is back POWER FAILURE 0 12 3 4 52s The elapsed time since the kit has been stopped is displayed Press the RES key to resume the kit at the point where it has been stopped or press the ESC key to go back to main menu RUN KIT 75 WASH ASP A Return to the main menu see 3 3 33 3 5 RINSE 3 5 1 Rinse with LP40 E RINSE ES OUT Connect blue tube on RINSE bottle ensure the plate carrier is not removed then press YES E Back to main menu See 3 3 3 5 2 Please RINSE with LP40 The message Is displayed to incite user to perform a RINSE operation when the washer has not been used during 10 minutes after a WASH operation Press YES Key to go back to main menu and launch a RINSE IMPORTANT The washer
48. ty protection when using hazardous substances or biological agents While the unit is connected to the power it is dangerous to operate without the protective cover Operation without the cover may be performed only by specialized and trained service personnel Read carefully the recommendations for optimized washing conditions provided in the appendix of this manual Contact your local representative if service is required SAFETY INFORMATION WARNING ELECTRIC S SHOCK HAZARD WARNING SPILLAGES WARNING INCORRECT OPERATION To operate the instrument must be mandatory connected to a wall socket equipped with earth The access to the mains supply input plug located on the instrument s rear panel must be kept free in order to use this plug if necessary as cut off device Although this instrument is fully insulated and grounded it is important for all users to be aware of the potential hazards of using liquids in close proximity to an electrical supply In the event of any spillage the instrument must be immediately isolated from the electrical supply and drained Spilt liquid should be removed as soon as possible Serious damage to the instrument may occur if fluid is allowed to penetrate the case The electrical supply MUST NOT be reconnected until the instrument has been fully inspected by a qualified technician or service engineer SERVICING MUST ONLY BE CARRIED OUT BY A QUALIFIED TECHNICIAN OR SERVICE E
49. uences ASP VERT POS Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of 1 8 0 1 is the highest needle position 15 0 is the lowest needle position Aspiration Vertical Position is applied for all Aspiration sequences 23 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued a Bottom aspiration continued BOTTOM ASP continued Plate parameters continued BOT VERT POS Bottom Aspiration Vertical Position 0 1 to 15 0 mm step 0 1 mm see also fig 8 at the end of 1 8 0 1 is the highest needle position 15 0 is the lowest needle position Bottom Aspiration Vertical Position is the height of the aspirating needle during the aspiration sequence of the bottom aspiration ASP DOWNW SPEED Aspiration Downward Speed 0 to 9 step 1 speed min 0 speed max 9 Aspiration Downward Speed is applied at the first Aspiration sequence BOT DOWNW SPEED Bottom Downward Speed 0 to 9 step 1 speed min 0 speed max 9 Speed of all downward movements for all bottom sequences wash and aspiration BOT UPWARD SPEED Bottom Upward Speed 0 to 9 step 1 speed min 0 speed max 9 Speed of all upward movements for all bottom sequences wash and aspiration 24 1 8 Software parameters for Wash protocols continued 1 8 3 Parameter s definitions continued Ag Plate Agitation AGITATION The microplate is s

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