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        IOP:011.0D INSTRUMENT OPERATING PROCEDURE
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1.    will appear if the sensor is not fully connected to the meter  and or an Err 882  message will appear if the RDO cap is missing or not properly installed     I     Il     IV     Calibration    A     Remove the end cap from the calibration sleeve and remove the sponge from the  sleeve  Saturate the sponge with distilled water and squeeze the excess water out of the  sponge     B  Replace the sponge and the end cap    C Turn power ON    D  Meter should now be in the measurement mode  press f1 to select cal    E  Use the up or down arrows to highlight Air and press f3 to select    F  Rinse probe with distilled water  blot dry with lint free tissue  and attach  the calibration sleeve    G  Wait about 5 minutes for the RDO sensor to equilibrate    H  Press f3  start     I  Wait for the DO reading on the meter to stabilize and stop flashing  Once the  reading is stable  the meter will display    Reading is stable  Accept auto calibration  value       J  Press f2  cal done     K  Press f1  meas  to get into measurement mode    Measurements   A  Make sure the meter is reading in mg l  This can be changed with the Mode button    B  Rinse probe with distilled water and blot dry    C  If measurements are to be taken directly from stream or lake  attach the stainless steel  protective guard  which also serves as a weight    D  Insert probe into sample or lower probe to desired depth and press Measure to start the  measurement    E  When the AR icon stops flashing  record the dissolved
2.  oxygen and temperature  Press  Measure again to start next measurement    F  When finished with final measurement  rinse probe with distilled water and return  probe to the calibration sleeve  And turn off meter    Storage   A  For short and long term storage  leave the RDO cap on the sensor  keep it in the    calibration sleeve  making sure to wet the sponge with distilled water  and keep the  sensor out of direct sunlight     MAINTENANCE   See instrument manual   REFERENCE     User Manual Thermo Scientific Orion Star A223 Portable RDO DO Meter Instruction Sheet and User  Guide     This procedure has been reviewed and approved by the undersigned representatives of the U S   Fish and Wildlife Service and Fisheries and Oceans Canada   me 1  N k l    4        Dea e  REVIEWED APPROVED_  gt           ME  Field Supervisor  U S      DATE_1   7 13          
3. IOP 011 0D    January 3  2013    U S  Fish and Wildlife Service  Marquette Biological Station  3090 Wright Street   Marquette  Michigan 49855  U S A     and    U S  Fish and Wildlife Service  Ludington Biological Station  229 South Jebavy Drive  Ludington  Michigan 49431    and    Fisheries and Oceans Canada   Sea Lamprey Control Centre   1219 Queen Street East   Sault Ste  Marie  Ontario P6A 2E5  Canada    INSTRUMENT OPERATING PROCEDURE    INSTRUMENT    Dissolved oxygen meter  MODEL    Orion Star A223    MANUFACTURER     Thermo Fisher Scientific Inc     SERIAL AND PROPERTY NOS                             Model Number Location Serial number Property number Identifying number  Orion Star A223 MBS 3205 1  PRECAUTIONS     POTENTIAL INTERFERENCES    Alcohols greater than 5  hydrogen peroxide  commercial bleach  gaseous chlorine  and gaseous sulfur  dioxide can affect readings  Static electricity may affect reading     Excessive water in calibration storage sleeve     Note  The RDO cap is only good for 365 days  The countdown begins with first reading taken  Err 881    will appear when the RCO cap has expired     SAFETY    No Special precautions       PROCEDURES     I  Meter preparation  A  Battery installation or replacement of four alkaline AA size batteries    l  Use a screwdriver to remove the 2 screws on the back cover of the meter    2  Remove the battery chamber cover    3  Install the batteries according to the inscribed battery symbols on the inside of  the battery chambe
4. r  Make sure batteries are in correct polarity    4  Close the battery chamber cover and secure cover with the 2 screws    5  Press and release the POWER button on the front of the instrument  The LCD  will come on  if not  the replacement batteries may be bad    6  A battery life indicator will appear in the upper  left corner of the display  showing the remaining battery power    7  To turn off the unit  press and hold the POWER button for three seconds    B  Initial Sensor Probe preparation   1  NOTE    1  Do not touch the bottom of the RDO cap  optical window or spring contacts  on the RDO sensor    2  The silicone grease that is supplied with the RDO sensor should only be  used to lubricate the two O rings on the RDO sensor  Do not fill the RDO  cap with the grease    3  The RDO cap has an internal clock that counts down the 365 day lifespan of  anew cap  This countdown begins with the first measurement taken    2  Remove the protective shipping cap from the end of the RDO sensor    3  Lubricate the two O rings on the RDO sensor with a thin film of the silicone  grease provided    4  Align the arrow on the RDO cap with the arrow on the RDO sensor    5  Slide and press the RDO cap onto the RDO sensor until the cap is flush with the  sensor body   Do not twist the RDO cap  Once the cap is installed  it should  not be removed until a new cap is needed or troubleshooting needs to be  performed     6  Connect the RDO sensor to the 9 pin input on the meter  An Err 880 message 
    
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