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1. b 2mini sized gels or 1 midi sized gel RO IN OT Ul1 2 2 c 3mini sized gels NO Ul lt d 4mini sized gels or 2 midi sized gels Pre Programmed Methods BACK Select gel number and size Thermo Scientific Thermo Scientific Pierce G2 FastBlotter 21 Section 3 Pre Programmed Transfer Methods 22 Thermo Scientific Pierce G2 Fast Blotter 0 Choose program Std Semi dry constant parameter in box HL 60 00 min NO Ul lt d Std Semi dry 1 0A H Note For Std Semi dry program d use Towbin transfer buffer or other conventional transfer buffer Choose Program 1 mini gel Std Semi dry 1 0A BACK 1 5mm Gel 25V 10 00 9 Select the Start button to begin transfer Transfer Ready EE Std Semi dry 25v 10A 60 00 _ Constant Limit Time N A After running at high current the anode and cathode plates can become hot Use caution when separating the gels and stacks from the plates Thermo Scientific Thermo Scientific Custom Methods Section 4 Custom Methods Modifying Pre Programmed Methods P re programmed methods can be modified before Start by selecting the Modify button Im 10A 60 00 _ Constant Limit Time h 1 Pressing the Select Constant V or A button will toggle the constant variable parameter from amps to volts or volts to amps Hody rae 60 00 1 0A 60 00 Constant me Select Const2. When continuously processing multiple samples at 5A for a maximum of 2 hours allow the cassette to cool for 30 minutes or use multiple cassettes to avoid excessive cassette heating Thermo Scientific Pierce G2 Fast Blotter 19 Section 3 Pre Programmed Transfer Methods 20 Thermo Scientific Pierce G2 Fast Blotter Traditional Semi Dry Transfer Method Simultaneously transfer one to four mini sized gels or one to two midi sized gels in 45 60 minutes Gels simultaneously transferred must have the same formulation l Equilibrate gel s for 15 minutes in Towbin transfer buffer 25mM Tris 192mM glycine 20 methanol Cut two extra thick 2 48mm thick sheets of Western blotting filter paper and one sheet of nitrocellulose or PVDF membrane to the same size as gel s Note The transfer stack should contain one sheet of 2 48mm thick filter paper on the bottom anode followed by membrane gel and one sheet of 2 48mm thick Western blotting paper on top Do not exceed 2 48mm total filter paper thickness for the bottom of the stack or 2 48mm total filter paper thickness for the top of the stack Use the appropriate Thermo Scientific filter paper Product No 88605 2 48mm thick 7 0cm x 8 4cm mini size Product No 88610 2 48mm thick 8 5cm x 9 0cm Product No 88615 2 48mm thick 8 0cm x 13 5cm midi size Product No 88620 2 48mm thick 20 0cm x 20 0cm Equilibrate filter paper and membrane in Towbin transfer buffer for
3. the power switch to the Off position 14 Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific Thermo Scientific Pre Programmed Transfer Methods Section 3 Pre Programmed Transfer Methods Fast Blotting Constant Amps Surface Area Fast blotting methods such as the Pierce G2 Fast Blotter require a high current power supply and an optimized high ionic strength transfer buffer such as Pierce 1 Step Transfer Buffer By increasing the current excellent transfer efficiency can be achieved in a much shorter time Amperage is held at a constant rate based on the surface area of the transfer stack s 22 23mA cm2 and voltage is limited to a maximum of 25V Pierce G2 Fast Blotter 1 mini gel 1 3A constant 25V limit 1 20 1 00 0 80 Volts o Q 0 60 amp Amps 0 40 Time minutes Figure 3 1 Fast blotting protocol volts vs amps Thermo Scientific Pierce G2 Fast Blotter 15 Section 3 Pre Programmed Transfer Methods Traditional efficient semi dry transfer protocols use constant voltage 25V and low ionic strength transfer buffer Towbin to generate low amperage for an extended length of time 30 60 minutes Traditional Semi Dry 1 mini gel 25V constant 1 0A limit m CTO 1 00 0 80 Volts o Q 0 60 amp Amps 0 40 0 20 0 00 Time minutes Figure 3 2 Traditional semi dry blotting protocol volts vs amps 16 Thermo Scientific Pierce G2 Fast Blotter Thermo Sci
4. PVDF membrane was not pre wetted with methanol or ethanol Air bubbles trapped between gel and membrane Filter paper used in the fast transfer exceeded 1 8mm Inefficient transfer of Inefficient binding of some low molecular weight low molecular weight proteins to PVDF proteins lt 25kDa to PVDF membrane 32 Thermo Scientific Pierce G2 Fast Blotter Solution Rinse the cassette top and bottom under warm water while removing any sticky salt residue with a gloved hand Briefly rinse with deionized water and place ina rack to dry For more thorough cleaning immerse the unassembled cassette in warm water use a gloved hand or clean sponge to remove any sticky salt residue Rinse with deionized water and place perpendicular in a rack to dry NOTE Failure to keep cassette top cathode and bottom anode clean will result in the sticking of moving parts and lead to poor transfer efficiency Equilibrate membrane and filter paper in Pierce 1 Step Transfer Buffer before transfer for a minimum of 5 minutes Use a sufficient amount of buffer for the equilibration step Increase transfer time from 7 10 minutes to 10 12 minutes Wet PVDF membrane with methanol or ethanol and equilibrate for 10 15 minutes in Pierce 1 Step Transfer Buffer before transfer When assembling transfer stack use a roller or pipette to remove any air bubbles between the gel and the membrane Use filter paper lt 1 8mm thick Combine ethanol an
5. Scientific Pierce G2 Fast Blotter 11 Section 2 Instrument Blotter Images with Feature Locations Color LCD Touchscreen Cassette Guide Rails SCIENTIFIC Control Unit Contact Points Figure 2 1 Blotter Control Unit front and side views detailing main components of control unit Cathode Anode Cassette Contact Points Figure 2 2 Cassette view detailing the upper cathode the lower anode and the electrical contacts that insert into the control unit contacts 12 Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific Instrument Section 2 Cassette Control Unit Figure 2 3 Contol unit with cassette inserted USB Power Switch Power Cord Connector Cooling Fan Figure 2 4 Rear view of control unit with cassette inserted Thermo Scientific Thermo Scientific Pierce G2 Fast Blotter 13 Section 2 Instrument Start Up Turn the power switch located atthe bottom rear of the blotter panel to the On position The power switch provides power to all of the blotter components The on board computer boots up the touchscreen displays the Thermo Scientific logo followed by Welcome to the Pierce G2 Fast Blotter and then automatically moves to the Main Menu The blotter is now ready for operation Shut Down On the Main Menu screen touch the Shut Down button When prompted Are you sure you wantto shut down select Yes When notified that It is now safe to turn off the device turn
6. cleaning product 1 Spray a soft cloth or paper towel with cleaning solution e g general purpose cleaner containing water and mild detergent Isopropanol or 70 ethanol Avoid abrasives and organic solvents 2 Wipe the exterior surfaces of the control unit and cassette Use caution to avoid touching the LCD touchscreen with anything other than a screen cleaning wipe 3 Ensure that the cooling fan vent is free of dust and debris Thermo Scientific Thermo Scientific Maintenance Section 5 Cleaning the Anode and Cathode Plates IMPORTANT Always wear gloves when touching the anode and or cathode Note Pierce 1 Step Transfer Buffer is a highly concentrated salt solution Build up of buffer salts will reduce cassette function and prevent the cassette from properly opening and closing 1 After each use thoroughly rinse the anode and cathode under warm water while removing any sticky salt residue with a gloved hand If the cathode has excessive salt build up use a mild acetic acid solution 596 to remove the residue 2 Rinse with deionized water and stand parts in a rack to dry Thermo Scientific Pierce G2 FastBlotter 31 Section 5 Maintenance Troubleshooting Problem Possible Cause Cassette is difficultto Salt deposited on moving open parts inside cassette Inconsistent transfer Membrane or filter paper was insufficiently equilibrated in Pierce 1 Step Transfer Buffer Insufficient transfer time
7. guidelines for proper set up before installation Locate the imager away from water solvents corrosive materials strong magnetic fields and vibration sources Space Requirements The blotter requires a stable laboratory bench or table Provide a minimum clearance of 3 Inches 7 62cm at the rear of the blotter to allow for adequate ventilation for cooling and access to the power cord and switch In addition allow sufficient space at the front of the blotter for docking and removing the cassette with from the control unit Table 2 1 Control unit dimensions Dimensions Inches Centimeters Width 6a Table 2 2 Cassette dimensions Dimensions Inches Centimeters Dim 13 39 Thermo Scientific Thermo Scientific Pierce G2 FastBlotter 9 Section 2 Instrument 10 Thermo Scientific Pierce G2 Fast Blotter Electrical Requirements Note A grounded circuit capable of delivering the appropriate current and voltage is required for installation Electrical requirements can be located on the rear panel of the blotter The blotter electrical system will adjust to the proper voltage for the respective country Connect the power cord to the rear left side panel of the device and plug into a grounded power outlet WARNING After running at high current the anode and cathode plates can become hot Use caution when separating the gels and stacks from the plates When continuously processing multiple samples at 5A
8. SCIENTIFIC Version 1 May 2013 2013 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Scientific Thermo Scientific Contents SSCUON Bi pP 5 Oa CU ani cen Cre cre cece DIRE CEDE CEDE EE CD REEF EC E EHE YD KE 5 Contacting Thermo Fisher Scientific 5 instrument OV SI VIOW Unpacking and Setup INS UOS oops E c Up dann QU rar ORBE Vs urs c SO iei P M MM Ne 9 Ins UMEN sisanra EC DD E HEP aE 9 Installation CRI Ts Tes NER RITE 9 S pace Requirements nennen 9 Electrical Requirements EDT TOT 10 Environmental Requirements eeeeeennnnnnnnnnnnnnennn enn 11 Blotter Images with Feature LOCationS usicusnatutturerh harsbid Scoto iE Ar mE RUND FERE DENS 12 indc 14 eibi 14 S Lori ON i t 15 Pre Programmed Transfer Methods nennen 15 Fast Blotting Constant Amps S urface Area sseeeeennnennnennnnnnnnnnnnnnn 15 Pierce 1 Step Transfer Buffer coesicesesin tnter nb ati x Ee rv KE Pru RUE rer uad dri ERA 17 Traditional Semi Dry Transfer MetfOQ usce toe p a ome oreste reped ea r
9. Start Guide Blot Roller Power Cord with C13 Connector Included Accessories Cassette Pierce G2 Fast Blotter Cassette User Interface The user interface consists of a color LCD menu touchscreen on the top of the control unit From the Main Menu five selections are available Touching the lower right Down arrow allows you to see the last two methods and the Setup button Main Menu D Shut Down W Pre Programmed Methods C Recent Methods Custom Methods Main Menu C Recent Methods Custom Methods 6 Setup Thermo Scientific Pierce G2 Fast Blotter 7 Section 1 Overview 1 Pre Programmed Methods This feature allows you to quickly access the recommended amperage voltage and time for the size and number of gels that you intend to simultaneously transfer The blotter is only intended for use with the following consumables e Pierce 1 Step Transfer Buffer e Bio Rad Trans Blot Turbo Transfer Stacks e Towbin Transfer Buffer for Standard Semi Dry Transfer only 2 RecentMethods Access to recently run methods listed from most to least recent 3 Custom Methods Create run and save a custom transfer method 4 Setup Control audio settings view software and hardware versions and install software updates to the user interface 5 Shutdown Safely shuts down the device 8 Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific section 2 Instrument Installation Guidelines IMPORTANT Review and implement
10. ant V or A Y v Done 2 Highlight the variable for change and press the Up or Down arrow to raise or lower the selected variable s value Thermo Scientific Pierce G2 Fast Blotter 23 Section 4 Custom Methods 3 After the program is modified press the Done button Modify Method Modify 1 0A 60 00 poy 1 1A 60 00 o mme Select m EE 4 The Review Method screen is now displayed Press Run Without Saving or Save a Run Without Saving will prompt the Transfer Ready Screen Press Start to begin the modified method b Ifyou wish to save the modified method press Save and use the alphanumeric key pad to enter up to 15 characters to identify the new custom method Press Done and then Start to begin the method The custom method will be saved in Custom Methods in the Main Menu Review Method 25V 2 0A 5 00 Limit Constant Run Without Saving HI Review Method 25V imi Constant Run Without Saving iL iL 24 Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific Custom Methods Section 4 im Sink ois WEN Transfer Read zF Electrol l I Main Menu 25V 5 00 Creating a New Custom Method Custom methods are typically used when transferring non standard gel sizes i e not mini or midi sized For rapid transfer protocols using Pierce 1 Step Transfer Buffer measure the total surface area of the gel s th
11. at least 5 minutes Note PVDF membrane must be wetted with methanol or ethanol before equilibration in Towbin transfer buffer Note Filter paper and membrane stacks can be prepared ahead few hours to overnight and stored at 4 C Assemble stack on anode as depicted in Figure 3 4 Center stack s on anode to ensure even pressure Use blot roller to remove any trapped air bubbles Note Removal of trapped air bubbles is essential for high quality transfer Two firm passes with the included blot roller is typically sufficient Note If assembling more than one stack on the anode surface evenly space and center the sandwiches so the top cathode surface applies even pressure to the surface areas of the stack s Ensure there is a 1cm space around all stack edges to allow any gases to escape during transfer Thermo Scientific Pre Programmed Transfer Methods Section 3 Cathode 1 sheet of pre wet filter paper thickness not to exceed 2 48mm Gel Membrane 1 sheet of pre wet filter paper thickness not to exceed 2 48mm Anode Figure 3 4 Orientation of filter paper membrane and gel in transfer stack for standard semi dry protocol 5 Gently press down top of cassette cathode to lock into place Slide cassette into the control unit 6 Select Pre Programmed Methods in the Main Menu 7 Selectthe number of gels and gel size mini or midi you wish to transfer NO lt a lmini sized gel 5
12. at you are transferring and multiply by 23mA cm2 For example if transferring a 12cm x 15cm non standard gel measuring the gel and not the cassette then use you would use the following formula 12cm x 15cm x 23mA cm 1000mA A 4 1A constant The voltage limit will be set at 25V The amperage will be constant and set at 4 1A The time will be set at 7 minutes for mid range molecular weight proteins 25 150kDa Note Five amps is the maximum current setting allowed If the gel area exceeds 220cm2 set the amperage to 5A and compensate by increasing the transfer time by 1 2 minutes For standard semi dry protocols using Towbin buffer voltage is held constant at 25V amperage is limited to 1 0A and time is set to 30 minutes to 1 hour Standard semi dry programming does not take surface area into consideration 1 Tocreate a custom method press the Custom Method button on the Main Menu 2 Select the Create Method button and press Select Constant V or A to toggle the constant variable parameter from amps to volts or volts to amps Thermo Scientific Thermo Scientific Pierce G2 Fast Blotter 25 Section 4 Custom Methods 26 Thermo Scientific Pierce G2 Fast Blotter Custom Methods Done 3 Highlight the variable for change and press the Up or Down arrow to raise or lower the selected variable s value New Method Done 5 The Review Method screen is now displayed Select Run Without Saving or Sa
13. d Pierce 1 Step Transfer Buffer in a 15 85 ratio before equilibrating filter paper and membrane e g 15mL of ethanol with 85mL of Pierce 1 Step Transfer Buffer Thermo Scientific Find us on Facebook THERMOSCIENTIFIC COM PIERCE 2023 THERMO FISHER SCIENTIFIC INC ALL RIGHTS RESERVED FACEBOOK IS A REGISTERED TRADEMARK OF FACEBOOK INC ALL OTHER TRADEMARKS ARE THE PROPERTY OF THERMO FISHER SCIENTIFIC INC AND ITS SUBSIDIARIES LIFE SCIENCE RESEARCH AFRICA BELGIUM EUROPE M NETHERLANDS 076 SU 32 IDDLE EAST 560 32 53 65 72 8 SWITZERLAND 0800 SL Sl FRANCE D 80D 50 amp s 15 5D GERMANY 02328 923225550 UK D8DD 252 285 EMAIL PERBIO EUROMARKETINGGSTH ERMOFISHER COM 3 lotter THERMOSCIENTIPIC COM PI EMAIL ERCE PIERCE CS THERNOFISHER con FOR OTHER REGIONS THERMOSCIENTIFIC COM PI VISIT ERC THERMOSCIENTIFIC COM PI ERCEDISTRIBUTORS USA 825 958 D757 OR 500 875 3723 CUSTOMER ASSISTANCE 2162527 0 05 13 Printed in China Thermo Scientific Thermo SCIENTIFIC Part of Thermo Fisher Scientific
14. e number of gels and gel size mini or midi you wish to transfer Pre Programmed Methods Back Select gel number and size Thermo Scientific Thermo Scientific Pre P rogrammed Transfer Methods Section 3 8 Choose the appropriate program to run constant parameter in box a Low MW 25kDa 25V 5 00 min b Mixed Range MW 25 150kDa 25V 7 00 min c High MW gt 150kDa 25V 10 00 min e 1 5mm thick gels or unknown size gels 25V 10 00 min Note For fast blotting programs a b c and e use Pierce 1 Step Transfer Buffer Transfer time may be increased to 12 minutes for extremely high molecular weight proteins or for slow transferring gels Do not use the Std Semi Dry transfer program d with Pierce 1 Step Transfer Buffer Choose Program 1 mini gel Low MW 25V 5 00 Mixed Range MW 25V 1 3A 7 00 High MW 25V 3A 1000 V 9 Selectthe Start button to begin transfer i a 25V 13A 5 00 Limit Constant Time N Modify Start 10 Upon transfer completion remove the transfer stack from the cassette s and thoroughly rinse the top and bottom section of the cassette Note Build up of buffer salts will reduce cassette function and prevent the cassette from properly opening and closing Rinse cassette s after every use A WARNING After running at high current the anode and cathode plates can become hot Use caution when separating the gels and stacks from the plates
15. entific Pre Programmed Transfer Methods Section 3 Pierce 1 Step Transfer Buffer Simultaneously transfer one to four mini sized gels or one to two midi sized gels in 5 10 minutes Gels simultaneously transferred must have the same formulation Mini gels are defined as 46cm and 60cm2 Midi gels are defined as 110cm Note that these dimensions are the surface area of the gel with the gel fingers removed not of the cassette 1 Cutfour sheets of 0 83mm thick Western blotting filter paper and one sheet of nitrocellulose or PVDF membrane to the same size as the gel s Note The transfer stack should contain two sheets of 0 83mm thick filter paper on the bottom anode followed by membrane gel and two sheets of 0 83mm thick Western blotting filter paper on top If 0 83mm thick filter paper is not available do not exceed 1 8mm total filter paper thickness for the bottom of the stack or 1 8mm total filter paper thickness for the top of the stack Use the appropriate Thermo Scientific filter papers Product No 84873 0 83mm thick 7 0cm x 8 4cm mini size Product No 84874 0 83mm thick 8 0cm x 13 5cm midi size Product No 88600 0 83mm thick 8 0cm x 10 5cm 2 Equilibrate filter paper and membrane in Pierce 1 Step Transfer Buffer for a minimum of 5 minutes Use sufficient buffer to completely cover the filter paper and membrane Note PVDF membrane must be wetted with methanol or ethanol before equilibration in Pierce 1 Step Trans
16. er and increasing the current amps A cm flowing through the transfer stack The system has been verified to work with commonly used pre cast and homemade SDS PAGE gels The Pierce G2 Fast Blotter can also be used for standard semi dry transfer protocols with Towbin buffer Contacting Thermo Fisher Scientific For technical or service assistance on the Pierce G2 Fast Blotter U S Customers 1 800 874 3723 Fax 1 800 842 5007 International Customers 1 815 968 0747 Fax 1 815 968 4736 Thermo Scientific Pierce G2 Fast Blotter 5 Section 1 Overview Technical Support email for U S and international customers outside Europe pierce ts thermofisher com Technical Support email for European customers perbio eurotech thermofisher com For more information about the Thermo Scientific P rotein Biology product line please visit thermoscientific com pierce 6 Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific Thermo Scientific Overview Section 1 Instrument Overview Unpacking and Setup Instructions Unpack your new Pierce G2 Fast Blotter Control Unit and or Cassette Verify that all of the accessories listed below are included with your blotter package Please note that the control unit and the cassette can be purchased separately or combined Your blotter purchase includes the following accessories each quantity 1 Included Accessories Control unit Pierce G2 FastBlotter Control Unit Quick
17. fer Buffer After electrophoresis remove gel from cassette s and briefly place in a tray containing deionized water or transfer buffer This will ensure even wetting facilitate proper gel placement and improve contact with the membrane 3 Assemble stack centered on the bottom half of the cassette anode as depicted in Figure 3 3 Use a blot roller to remove any trapped air bubbles Note Removal of trapped air bubbles is essential for high quality transfer Two firm passes with the included blot roller is typically sufficient Note If assembling more than one stack on the anode surface evenly space and center the sandwiches so the top cathode surface applies even pressure to the surface areas of the stack s Ensure there is a 1cm space around all stack edges to allow any gases to escape during transfer Thermo Scientific Thermo Scientific Pierce G2 Fast Blotter 17 Section 3 Pre Programmed Transfer Methods 18 Thermo Scientific Pierce G2 Fast Blotter Cathode 2 sheets of pre wet filter paper combined thickness not to exceed 1 8mm Gel Membrane 2 sheets of pre wet filter paper combined thickness not to exceed 1 8mm Anode Figure 3 3 Orientation of filter paper membrane and gel in transfer stack 4 Gently press down top of cassette cathode to lock into place 5 Slide cassette into the control unit 6 Select Pre Programmed Methods in the Main Menu 7 Using the touchpad select th
18. for a maximum of 2 hours allow the cassette to cool for 30 minutes or use multiple cassettes to avoid excessive cassette heating WARNING Blotter use outside of the workflows described in this manual may put the operator at risk of dangerous exposure to electrical shock Do not use this instrument for any purposes or in any configurations not described in this manual WARNING The blotter control unit can contain dangerous electricity and is not designed to be opened by the user Disconnect all power to the control unit before maintenance by a qualified technician WARNING Do not overfill the cassette with liquid Excess liquid can overflow into the control unit and possibly cause electric shock Follow the appropriate instructions for reagent amounts and empty any remaining liquid in the cassette upon run completion Table 2 3 Blotter electrical ratings Blotter Electrical Rating Parameter Supply Voltage VAC 120 240 Frequency Hz 50 60 Maximum Power Rating 168 W Fuse Power Center T4AL 250V 4A Thermo Scientific Thermo Scientific Instrument Section 2 Table 2 4 Blotter electrical requirements for major countries Region VAC Frequency Hz Amps A US and Canada Environmental Requirements For optimal performance maintain a constant laboratory temperature range of 15 25 C 59 77 F with relative humidity between 20 70 Do not place blotter in direct sunlight Thermo
19. tts hour 1 Pierce 1 Step Transfer Buffer is intended to rapidly transfer protein from gel to membrane using a high transfer current based on transfer area and a short time 5 10 minutes a Do notexceed 12 minute transfer times with rapid transfer protocols b Donotexceed 22 23mA cm current surface area with rapid transfer protocols 2 Conventional transfer buffers such as Towbin buffer 25mM Tris 192mM glycine 20 methanol are low ionic strength buffers and transfer time ranges from 45 60 minutes Current normally spikes and then quickly drops to a very low level while voltage reaches its maximum 25 volts Thermo Scientific Custom Methods Section 4 Thermo Scientific Thermo Scientific Pierce G2 Fast Blotter 29 Section 5 Maintenance 30 Thermo Scientific Pierce G2 Fast Blotter Maintenance Cleaning the Blotter LCD Touchscreen IMPORTANT Use of ammonia based cleaners to clean the touchscreen is NOT recommended Do not spray cleaner directly onto the LCD screen IMPORTANT Do not press hard while cleaning the screen Do not leave excess cleaning solution on the LCD screen as this may cause long term damage 1 Usea pre moistened LCD screen cleaning tissue to clean the surface of the touchscreen Cleaning the Blotter Housing and the Cassette IMPORTANT When using cleaning products with the blotter consult the appropriate material safety data sheet MSDS to confirm compatibility and proper usage of the
20. v b ennt bos n 20 SOC CON D FQ OREL ROREUERERUTEUTE E ER E V REX 23 CUSTOM MEUOUS oca niri pd OCDE REC EOD E GU OE EE RECO OO ERE 23 Modifying P re P rogrammed Methods eeeennnnnnnnnnnnnnnnnnnnne 23 Creating a New Custom Method nennen 29 Recommended Maximum Watts Hour Limit ennn nnn 28 SSC OND wid pd SERNEHOR DOGS TREEDG DC ER REDARU POESIE ERR D DIR 30 Mallitenalie nsn nic eda OR NoD DEDE XE EE OE on DVD FEEDER 30 Cleaning the Blotter LCD Touchscreen aiasodesadusiv verant del EVEN LY E E 30 Cleaning the Blotter Housing and the Cassette nnn 30 Cleaning the Anode and Cathode Plates 31 Troubleshooting assctuvepresrttd apre vlt Rouen Dentro cR UE RED EEUS 32 Thermo Scientific Pierce G2 Fast Blotter 3 Thermo Scientific Overview The Thermo Scientific Pierce G2 Fast Blotter is designed for rapid semi dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes Traditional Western blotting techniques require a transfer of one hour to overnight to achieve good transfer efficiency When used in conjunction with Thermo Scientific Pierce 1 Step Transfer Buffer the Pierce G2 Fast Blotter is designed to provide transfer efficiency in 5 10 minutes that is equivalent to or better than traditional blotting techniques without the need for gel pre equilibration This significant reduction in protein transfer time is accomplished by optimizing the ionic strength of the transfer buff
21. ve d Run Without Saving will prompt the Transfer Ready screen Press Start to begin the modified method If you wish to save the modified method press Save and use the alphanumeric keypad to enter up to 15 characters to identify the new custom method Press Done and then Start to begin the method The custom method will be saved in Custom Methods in the Main Menu Thermo Scientific Custom Methods Section 4 i 2 L 4 i E s k i i Modify Thermo Scientific Thermo Scientific Pierce G2 Fast Blotter 27 Section 4 Custom Methods 28 Thermo Scientific Pierce G2 Fast Blotter Recommended Maximum Watts Hour Limit To prevent damage to the cassette or control unit custom methods are limited to 25 watts hour when modifying pre programmed methods and or creating a new method Any voltage or amperage adjustments that result in exceeding the maximum 25 watts hour will bring up an alert triangle E amp A on the Up arrow key Continuing past this value will eventually result in a Watt Hour Limit warning window Modify Method 55V 104A 60 00 25 V EI 60 00 Select Constant V or A Ay Watt Hour Limit Increasing current will exceed the watt hour limit Please reduce voltage or time value s before increasing current If the Watt Hour Limit warning window appears select the Okay button to return to the previous screen and decrease appropriate number to return to a watt hour value less than 25 wa
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