EF10100 Electrophoretic KIT EXPRIME 72 ENG.
Contents
1. for reagents dilution PRECAUTIONS Reagent may contain not reactive and conservative components It is opportune to avoid contacts with the skin and do not swallow Perform the test according to the general Good Laboratory Practice GLP guidelines Pay attention to hazard symbols and R and S on the labels Consult the MSDS PREPARATION AND STORAGE OF REAGENTS The reagents not contaminated when stored at 4 30 C in the dark are stable until the expiration date on the label The Staining is supplied ready for use Buffer is provided concentrated 10 X Dilute 1 10 with distilled water The diluted buffer is stable 20 days at 15 25 C or 35 days at 2 8 C Destaining is provided concentrated 20 X Destaining can produce a precipitants that are redissolved by water bath at max 50 TC Dilute 1 20 with distilled water The diluted Destaining not contaminated is stable until expiry date stated on the label PROCEDURE Loading Reagents Place the bottle of Staining reagents on the instrument compartment 72 EXPRIME ensuring the inclusion of the loading pipe marked with the word STAIN The contents of the vial is sufficient for the correct color of 25 cellulose acetate membranes Fill the room electrophoresis instrument until to indicated level approx 150 ml with Buffer diluted previously prepared Replace the buffer diluted every 25 tests or in case of any mold Insert into the slots of the electropho2. Meridiagkhlealtincare TEST SUMMARY The proteins in the samples are separated in the appropriately buffered by electrophoretic migration The result is achieved by depositing a serum sample on a appropriate support of cellulose acetate with hard plastic material and application of electric current to a preset time The protein fractions are positioned individually in different areas of the strip according to their specific electrical charge Once correctly completed electrophoretic migration the strip should be treated with a reagent capable of highlighting the protein fractions and in this case you use dye Red Ponceau S At the end of the staining process is necessary to remove excess dye to make visible only the bands of protein fractions this is achieved by treating the strip with an appropriate destaining The strip is read by an optical scanner system The values are expressed as a percentage of the different fractions with the chart of the separation SAMPLES Serum not hemolized collected according to NCCLS H11 A3 document Stability 2 weeks at 2 8 C or 4 days at 15 25 C REAGENTS Buffer Tris Hippurate buffer Staining R P Ponceau S 0 2 p v Destaining Citric Acid 1 M I preservatives and stabilizers Electrophoretic stripes cellulose acetate 76 x 60 mm MATERIAL REQUIRED BUT NOT SUPPLIED EXPRIME 72 instrument Normal laboratory equipment Distilled water 5 liters tank or fit plastic containers
3. TED VALUES Albumin 3 6 4 9 g dl 55 64 Ot 0 2 0 4 g dl 2 2 4 5 Op 0 4 0 8 g dl 7 9 B 0 6 1 0 g dl 9 13 y 0 9 1 4 g dl 14 18 Every laboratory should establish own reference intervals in accordance with own population NOTES e Use only throwaway materials for to avoid contaminations e f the results are incompatible with clinical presentation they have to be evaluated within a total clinical study e Only for IVD use CALIBRATION QUALITY CONTROL It is suggested to perform an internal quality control with Control Serum of serum proteins TEST PERFORMANCE All the performance of this reagent were established on the instrument EXPIME 72 using reagents Methods comparison 46 samples of normal and pathological sera were analyzed by electrophoretic LTA KIT EXPIME on 72 and the results were compared with those obtained with a similar system commercially available The LTA has confirmed KIT electrophoretic paths equivalent to those of the reference system with a 100 agreement Interferences In Hemolyzed serum free hemoglobin bound by Haptoglobin interferes with the assay of Fraction Alpha 2 Are described in the literature various drugs and other substances that may interfere with the determination Precision Sample 1 B04 0422 416 Sample 2 o B 1079 o7 387 Ooo y o 2442 on7 29 WASTE DISPOSAL Product is intended for professional laboratorie
4. cniche di Chimica clinica p 672 MANUFACTURER laboratorio Meridian Healthcare srl Tel 39 095 725 68 69 Fax 39 095 725 44 54 info meridianhealthcare it www meridianhealthcare it SYMB OLS Only for IVD use Lot of manufacturing lt Code number Storage temperature interval Expiration date Warning read enclosed documents Read the directions e aa E Biological risk Mod 01 06 ver 1 3 24 01 2011
5. resis chamber the Felts included in the kit previously immersed in buffer for 1 minute ELECTROPHORETIC KIT EXPRIME 72 Qualitative and quantitative determination of serum proteins 5 fractions on EXPRIME 72 instrument Insert the intake hose necessary to Destaining into the tank containing the reagent previously diluted Make sure when using the level of the tank never falls below 500 ml Also make a 5 litres tank containing distilled water CLEAN required for cleaning tool and 1 empty tank necessary to drain WASTE Assemble the electrophoretic strip on the appropriate frame avoid handling the surface WARNING It is recommended to verify the correct and complete tightening of carrier strips Put the frame in the strips rack framed of the instrument starting from position 1 Place the filter paper into the slots For instruments equipped with automatic loading and unloading of reagents fill the pan with the respective reagents diluted For details see the user manual of the instrument Loading Samples Pipet 25 30 ul of fresh serum not hemolyzed each well of the base sample holder ensuring a homogeneous distribution and without bubbles Operations Parameters Constant current Optics Compensation IMBIBITION DRYING 1 MIGRATION STAINING DESTAINING 1 DESTAINING 2 DRYING 2 DEPOSITION TIME O 120 second o0 second 720 seconds 1 20 150 second a20 second 300 second s second 20 second EXPEC
6. s Waste products must be handled as per relevant security cards and local regulations PACKAGING CODE EF10100 400 TESTS Buffer 1x100mi liquid Destaining 2x250 ml liquid Staining 2x100ml liquid Electrophoretic stripes 2 x 25 pc Imbibition set 4 pc Paper set 10 pc CODE EF10110 200 TESTS Buffer 1 x 50 ml liquid Destaining 1x 250ml liquid Staining 1x100mi liquid Electrophoretic stripes 1 x 25 pc Imbibition set 2 pc Paper set 5 pc REFERENCES Young DS Effects of drugs on Clinical Lab Test 4 ed AACC Press 1995 Young DS Effects of disease on Clinical Lab Test 4 ed AACC Press 1999 M Paget e Coustenoble Microelectrophor se des prot ins du s rum sur acetate de Cellulose G latineux Ann Biol Clin 23 10 12 1209 1219 1965 Kenz Stato and Haruko Kasai Japan Electrophoresis of serum Proteins on gelatinized Cellulose Acetate Medicine and Biology 70 5 195 298 May 10 1965 Medicine and Biology 71 3 144 151 Spt 10 1965 B Colfs J Verheyden Rapid Method for Determination of Serum Haptoglobin Clin Chim Acta 12 470 472 1965 G Rapi V Frangini C Maggi D Pratesi Elettroforesi e Microelettroforesi R adiale su gel di acetato di Cellulosa Proteine e Emoglobine Riv Clin Pediatra Vol 78 n 6 785 800 1966 J Kohn abstracts of VI Inter Congr Clin Chemistry see page 203 204 S Karger A G Basel 1966 F Pasquinelli Diagnostica e Te
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