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Gel Logic 212 PRO User`s Guide

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1. 0002000 2 9 Exposure Section of the GL212 PRO Acquire Window esses 2 11 Buttons Section of the GL212 PRO Acquire Window 000200 2 13 Lens Section of the GL212 PRO Acquire Windows esses 2 13 Illumination Section of the GL212 PRO Acquire Windows 04 2 14 Emission Filter Section of the GL212 PRO Acquire Windows 2 15 The GL212 PRO Preferences Dialog Box 0 ireren eese 2 16 Setting Up Your System leeele eere 3 1 Environmental Requirements sseeeeeeeee ee 3 2 Blectrical Requirements sss esrin oes ASA CERE ERIS iR REC Rc dA 3 2 Space Requirements sss seprana bee aaah hae ieee eae 3 2 Installing the Carestream Molecular Imaging Software Windows 3 3 Uninstalling a Previous Version of MI or 1D Software Windows 3 3 p o h e ie 2 e 0 et 7 Carestream Molecular Imaging Software Installation Windows 3 5 Copy Protection Device Installation Windows 0 0 00 eese 3 6 Windows Power Settings 2o necesse geh ned dc ee OR e qu eae eae da 3 7 Installing the Carestream Molecular Imaging Software Macintosh 3 8 Uninstalling a Previous Version of MI or ID Software Macintosh 3 9 Carestream Molecular Imaging Software Installation Macintosh 3 10 Copy Protection Device Installation Macintosh
2. System Overview This section provides an overview of the Carestream Gel Logic 212 PRO Imaging System GL212 PRO You will review the principles of operation and get a better understanding of the critical components the Carestream GL212 PRO Imaging Cabinet with accessories and the Carestream GL212 PRO Acquire Software dp lt o 6 E O c E lt 6 Z 2 1 2 2 The Carestream GL212 PRO Imaging Cabinet The Carestream GL212 PRO Imaging Cabinet provides a a light tight environment for imaging It is equipped with built in white light epi illumination for imaging opaque samples like plates and colorimetric blots The built in UV transillumination provides broadband UV ideal for imaging fluorescent samples like ethidium bromide stained gels The White Light Transilluminator Accessory optional is an excellent way to image Coomassie gels or for densitometry of films In addition the Epi illumination Modules provides the illumination needed to image thin layer chromatography plates and fluorescent stained blots The cabinet accommodates samples up to 24 5 cm x 33 cm GL212PRO on A The GL212 PRO Camera is mounted in the top of the imaging cabinet The camera captures 1392 x 1032 pixel images at 12 bits per channel and image file accumulations greater than 14 bits B The Indicator Display indicates on off status of the system and what illumina tion source is operating C The Automated Filter Whe
3. 0 0 000000 ee eee 3 11 Macintosh Power Settings i a esee ed pede dae inek e eed Re 3 12 Launching Carestream Molecular Imaging Software for the First Time 3 13 Carestream MI Image Database 00 cece eee eee 3 16 Installing the Carestream Gel Logic 212 PRO Imaging System 3 20 Installing the GL212 PRO Imaging Hardware 000 0000 e eee 3 20 Carestream Gel Logic 212 PRO Accessories Installation 3 23 Installing the White Light Transilluminator Module Optional Accessory 3 23 Installing the UV Epi illumination Light Module Optional Accessory 3 25 Installing the UV Safety Shield 2 22 c0s sci sddsdsr eee ccee mesa e ees 3 27 Launching Carestream Molecular Imaging Software with your Carestream Gel Logic 212 PRO Imaging System 0 00 0 eee eee eee eee 3 28 Installing Emission Filters oec Sees ea sue acd ew age were ee 3 30 Focusing the Cameras 9 oe ov eiekie Hacked P RETO Reb D e P S 3 32 Capturing ImageS xu wes x 65 An R Ras tun can eR e RR m a n 4 1 Launching Carestream Molecular Imaging Software and the Gel Logic 212 PRO Acquire WindOW 2 sic ds a6 e b ERRERRE I RR LEER RAF RR aden es 4 2 Preparing for Image Capture sssseeeeeeeeeeee ee 4 4 Previewing and Capturing Images essas recio eiris pi ids eee 4 5 Custom Capture Settings cs ipee ke ti a aa e a doe EE ra d RR de 4 9 Saying Custom USer Settings r c cdd perde embark
4. The Exposure Time defines the exposure The minimum and maximum exposure times are 0 001 seconds per frame and 16 seconds per frame respectively The minimal increment is 0 001 second The Coarse and Fine Exposure arrows allow you to adjust the exposure time in either coarse or fine increments This eliminates the need for you to use your keyboard The nterval is the time interval between the beginning of one exposure and the beginning of the next exposure The minimum and maximum intervals are 5 seconds and 24 hours respectively Stop After determines when to stop time lapse captures You can set stop criteria based on experimental duration or by the number of frames The maximum duration time is 200 hours The maximum number of image frames that may be captured is 100 T To capture an image click Capture or Quick Print The Capture button initiates image acquisition Once acquired files can be analyzed annotated and printed The Quick Print button sends the image directly to your Quick Print printer and no copy of the image is saved Custom Capture Settings You can speed up the capture process by creating custom capture settings for the various types of experiments or specific personal preferences Once saved Carestream MI remembers the camera settings which include v Type v Exposure time v Number of exposures v Illumination correction v Image orientation V Preferences sele
5. Carestream Gel Logic 212 PRO User s Guide 1B5438180 Rev A 0409 Copyright Carestream Health Incorporated 2009 All rights are reserved No section of this manual may be photocopied reproduced translated to another language stored in a retrieval system or trans mitted in any form without the prior written consent of Carestream Health Incorporated The information contained in this manual is subject to change without notice Carestream Health Incor porated makes no warranty of any kind with regard to this written material Carestream Health Incor porated assumes no responsibility for any errors that may appear in this document Disclaimer While the Carestream Gel Logic system can be used for molecular imaging of materials a purchaser should be aware that the methods of preparing and viewing the materials for molecular im aging may be subject to various patent rights Apple FireWire Macintosh Mac Mac OS and OS X are registered trademarks and Safari is a trade mark of Apple Computer Incorporated Coomassie is a registered trademark of Imperial Chemical Industries Limited IEEE 1394 is a trademark of The Institute of Electrical and Electronics Engineers Standards Associa tion Internet Explorer Windows Windows Vista and Windows XP are registered trademarks of Microsoft Corporation Norton Utilities and Norton Antivirus are registered trademarks of Symantic Corporation SYPRO and SYBR are trademarks
6. Ce Problem Probable Cause Solution UV Transilluminator UV Epi lights are not operating No power The cabinet door safety switch is not engaging Either the bulbs need replacement or a ballast problem Check that the power cord on the back of the instrument is properly attached and that the outlet is supplying power Check that the main power switch on the back panel is set to the ON position Verify that the door is firmly closed and safety switch is engaged Test whether the bulbs or the ballast needs replacement replace the UV bulbs with similarly sized white light bulbs If the bulbs flicker the ballast needs changing If the bulbs don t flicker the UV bulbs need replacement Common Image Problems Problem Probable Cause Solution Image is black or too dark Not enough light for a good contrast exposure Increase the aperture setting using the Aperture button or increase the exposure time See Chapter 4 Capturing Images Image is white or too light Too much light during the exposure Decrease the aperture setting using the Aperture button or decrease the exposure time See Chapter 4 Capturing Images Image is blurred Image is not in focus Adjust the focus using the Focus buttons Run the Autofocus procedure See Using the Focusing Tool in Chapter 4 Capturing Images Dirty or cracked filter Capture an im
7. Ex NOTE With the software running select About MI under the Help menu Windows or select About MI under the Apple menu item Macintosh UY The type of computer you are using make model VY Operating system software version ES NOTE Check your operating system version by right clicking on the My Computer icon and then on Properties Windows or select About This Mac under the Apple menu item Macintosh UV The type of image you are capturing or analyzing w The problem you are having and what you were doing when the problem occurred Please note the exact wording of any error messages including any error numbers displayed Carestream Molecular Imaging Technical Support Contact Carestream Molecular Imaging Technical Support by Y Utilizing our World Wide Web support pages at mi carestreamhealth com w Calling Carestream Molecular Imaging Technical Support at In US and Canada 877 747 4357 or world wide 203 786 5657 between the hours of 8 00 a m and 6 00 p m Eastern Standard Time Monday through Friday w E mailing Carestream Molecular Imaging Technical Support at molecular support carestreamhealth com v Faxing Carestream Molecular Imaging Technical Support at 203 786 5656 Common Instrument Problems Problem Probable Cause Solution Cannot access the camera capture system Camera Computer Interface Cable and or Serial Cable are not properly connected Check the connections on t
8. or in the U S A contact the Electronics Industry Alliance web site at www eiae org The sound pressure level LA is less than 70 dB This Class A digital apparatus meets all requirement of the Canadian Interference Causing Equipment Regulations Cet appareil num rique de la classe A respecte toutes les exigences du R glement sur le mat riel brouilleur du Canada For Use in European Union WARNING This is a class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures 3 o gt m lt In the European Union this symbol indicates that when the last user wishes to discard this product it must be sent to appropriate facilities for recovery and recycling Contact your local representative or refer to http recycle carestreamhealth com for additional information on the collection and recovery programs available for this product For Use in Japan For Use in Taiwan a ur ERI EAA Med EEN REE Re gt BAR RE RATE ALTA T HFA Gk RR BAR o Appendix A Parts and Accessories The following is a listing of parts and accessories related to your Carestream Gel Logic 212 PRO Imaging System GL212 PRO For information contact Carestream Molecular Imaging Sales or your Carestream Molecular Imaging dealer Contact Carestream Molecular Imaging Sales by Y Utilizing our World Wide Web support pages at mi carestreamheal
9. which locks the bulb in place 9 Proceed with steps 7 and 8 above for the other bulbs 10 Replace the top Platen on the transilluminator base and reinstall the four 4 screws 11 Plug the power cord into the AC MAINS outlet and turn on the cabinet switch 5 5 5 6 Replacing the White Light Epi Illumination Bulbs Tools required v 2 5 mm Allen wrench Replacement parts v GL212 PRO White Light Bulb Kit 1 1 Turn off the cabinet switch 2 Remove the power cord from the AC MAINS outlet WARNING The power cord is the primary disconnect device from the AC MAINS outlet Always turn the switch the cabinet off and disconnect the unit from the AC MAINS outlet before performing any service 3 Open the GL212 PRO Imaging Cabinet door 4 Remove the bottom screw on the side of the bulb housing and rotate the retaining plate 180 clockwise 5 Slide the white diffuser cover towards the front of the cabinet to remove 6 Push the red button while simultaneously rotating the bulb counter clockwise and lift out to remove it from the bulb holder 7 Install a new bulb by pushing the base into the bulb holder until it snaps into place 8 Reinstall the diffuser cover by sliding it back into the frame 9 Rotate the retaining plate back into position 10 Install the retaining screw on the side of the housing 11 Repeat Steps 4 through 10 to replace the bulbs in the second light fixture 12 Plug the
10. 0 or greater web browser 5i e x je Q o ie Minimum System Requirements Macintosh WY Intel Macintosh with a USB port and IEE 1394 FireWire port v Mac OS 10 5 or greater wep NOTE Check your operating system version by selecting About This Mac under the Apple menu item v 17 in display 1280 x 1024 resolution v 2 GB recommended v Minimum of 10 GB of available hard disk space Y CD drive CD RW drive recommended v TCP IP VY Safari 3 0 or greater web browser 1 5 Technical Support For technical support contact Carestream Molecular Imaging Technical Support or your Carestream Molecular Imaging dealer For up to date dealer information visit our WEB site at mi carestreamhealth com When contacting technical support please have the following information available v The serial number of your GL212 PRO located on the back of the unit v The serial number of your GL212 PRO Camera press Control and T buttons simultaneously when the GL212 PRO Acquire window is displayed v The serial number and version number of your Carestream MI software Ex NOTE With the software running select About MI under the Help menu Windows or select About MI under the Apple menu item Macintosh v The type of computer you are using make model v Operating system software version ES NOTE Check your operating system version by right clicking on the My Computer icon and then on Propertie
11. Green SYBR Gold and Coo massie Blue Microtiter plate assays Colony plates Plaque screening Thin layer chromatography plates Y Generate a quick print of your image S pe r Z 5 Ke 3 D D 0 v Annotate analyze and print your images 4 1 Launching Carestream Molecular Imaging Software and the GL212 PRO Acquire Window Once you have set up your GL212 PRO and positioned your sample using the framing masks you are ready to launch Carestream MI to communicate with the camera and capture an image If you have not yet set up your system see Chapter 2 Setting Up Your System 1 Launch Carestream MI software by clicking on the MI icon found on the desktop 2 Choose Select Digital Camera from the File menu Choose GL212 PRO as the Digital Camera Select Digital Camera Digital Camera eco 2PRO MA Port Connection Speed 3 Carestream MI systematically searches the computer s ports for a digital camera v If you previously used a camera Carestream MI searches for that camera in the port last used v If you are a new user the software will first search for a IEEE 1394 FireWire port W If no camera is found the Select Digital Camera dialog box appears allowing you to manually specify the camera Make sure that the camera is connected Use the Digital Camera pop up menu to select your digital camera and click OK ES NOTE The Port and Connection Speed pop up menus will
12. SYBR Follow the destaining protocol of your dye s manufacturer or use MgSO 1mM to detain gel Sample not sufficiently stained Follow the manufacturer s staining and destaining protocol Fluorescent dye stain is expired or has been photobleached Check by placing a drop of the dye stain on the platen and preview with appropriate illumination source Exposure time not sufficient Increase Exposure Time Camera aperture too low Increase Aperture Sample overexposed all pixels are white or appear red on screen of saturation checkbox is selected Decrease Exposure Time and or Aperture Common Software Problems Problem Probable Cause Solution iChat opens when the Gel Logic camera is connected iChat preferences designate that iChat should automatically open when cameras are connected Open iChat from the Dock or by clicking Applications from the Go menu bar item and the iChat icon in the iChat folder Click Preferences from the iChat menu item Click the Video tab and uncheck the Automatically open iChat when camera is turned on checkbox My Macintosh computer is going to sleep mode System Display sleep mode set incorrectly Under Control Panel Energy Saver set the sleep setting to Never My Windows computer is going to sleep mode System Display sleep mode set incorrectly Click the Power Options icon from the Start men
13. amp FireWire 8683336 Desktop Macintosh w 20 Flat Panel Monitor amp FireWire 1296409 Sony UP D897 Thermal Printer 8650582 High Density Glossy Thermal Black and White Paper User s Guides 8848137 Gel Logic 212 PRO User s Guide English 1865476 Gel Logic 212 PRO User s Guide French 8701419 Gel Logic 212 PRO User s Guide German Software Upgrades 8177446 Molecular Imaging Software Standard 5 X to Network Upgrade 1537893 Molecular Imaging Software Standard 5 X to Regulatory Upgrade 1274596 Molecular Imaging Network Licenses 5 X Service Contracts 1624923 Gel Logic 212 On Site Premium Service Contract 1 year 1892884 Gel Logic 212 On Site Basic Service Contract 1 year 8903601 Gel Logic 212 Depot Service Plan 1 year 1091958 Gel Logic 212 Preventative Maintenance Service Plan 1 year Replacement Parts 1861848 Gel Logic PRO Epi White Light Bulb Kit 1 pk 1920362 Gel Logic 306 nm UV Transillumination Bulb Kit 4 pk 8295727 Gel Logic 365 nm UV Transillumination Bulb Kit 4 pk 1125632 Gel Logic PRO Platen Replacement Kit 8480899 Gel Logic PRO Filter Tool 1209964 Gel Logic PRO Leveling Feet 4 pk 1573054 Gel Logic PRO FireWire Cable 1733823 Gel Logic PRO Serial Communication Cable Index A Acquire Software installation 2 6 2 19 3 3 3 12 launching 3 13 3 19 4 2 4 3 Preferences 2 16 2 19 windows 2 6 2 19 4 2 4 14 Acquir
14. copy protection device is configured for a newer version of MI You are using an older version of MI you may want to use a newer version instead Error 12002 The copy protection device is from an older version of MI Shut down your computer and attach the copy protection device that came with the newest version of Carestream MI Restart your computer Error 12002 A new copy protection device was expected but not found Shut down your computer and attach the copy protection device that came with the newest version of Carestream MI Restart your computer Error 12003 The copy protection device was disabled during the upgrade process Shut down your computer and attach the upgrade copy protection device that came with the upgrade package Restart your computer Return the disabled copy protection device for recycling Error 12004 Upgrading your MI requires both your existing and new copy protection devices Connect both your existing copy protection device and the upgrade copy protection device to your computer and restart the computer Warranty Warranty Time Period Carestream Health Inc warrants the Carestream Gel Logic 212 PRO Imaging System GL212 PRO to be free from malfunctions and defects in both materials and workmanship for one year from the date of purchase If a computer was purchased with the GL212 PRO it is covered under separate warranty pro
15. eee ee babes gee kode aes 6 5 Common Software Problems 0 00 c eee cece eee eee 6 7 Warranty Rm W 1 Limited Warranty Period crede RE UR ER GR b RR ages W 1 Days and Hours of Coverage W 1 Warranty Repair Coverage sse teretne ass doad eee ete nee W 1 Limitations etch AONE SORES AUREUS RH EE EES AERE W 2 How to Obtain Service 55 43 e RUDI PEERS oe oh ee See en EDS W 3 General Instructions for Cleaning and Decontamination For Return of the GL212 PRO 4 ilk e Er pre EE meri dnb ix ne W 4 General Cleaning Proc dure ees e rera RR cade CORREA W 4 Radiological Decontamination Procedure ssllsseeee eese W 4 Repackaging the GL212 PRO set baa Rb RE an EF GRE W 6 Regulatory Informati n esses eresi os iieii ostaria VE e W 7 Appendix A Parts and Accessories A 1 p o h e o m et gt e 7 Introduction Thank you for purchasing the Carestream Gel Logic 212 PRO Imaging System GL212 PRO The GL212 PRO in combination with Carestream Molecular Imaging Software Carestream MI is specially designed for gel documentation and analysis The IEEE 1394 FireWire 1 4 mega pixel CCD camera with an automated lens provides real time imaging for increased throughput in your laboratory The scientific grade CCD provides a 12 bit data file which can be accumulated up to 14 bit image files for improved sensitivity and data accuracy The GL212 PRO fully automa
16. for Turn off monitor Turn off hard disks System standby and System hibernates functions become Never 4 Click OK and proceed to Launching Carestream Molecular Imaging Software for the First Time Windows Vista Select System and Maintenance from the Start menu and the Control Panel 2 Click Power Options select to Create a power plan and choose High Performance from the list of protocols 3 Proceed to Launching Carestream Molecular Imaging Software for the First Time 3 7 3 CD F Ke c Ke 3 8 Installing the Carestream Molecular Imaging Software Macintosh Any prior versions of MI 1D or camera software that are loaded on the computer must be uninstalled prior to installation If you are a new user proceed to Carestream Molecular Imaging Software Installation Macintosh Macintosh OS X installations may require authentication of permissions If you have purchased the Carestream Molecular Imaging Software Network Edition Carestream MI NE to use with your Carestream Gel Logic 212 PRO Imaging System v Follow the instructions in the Carestream Molecular Imaging Software Network Edition Administrator s Manual to install Carestream MI NE Software v Then proceed to Launching Carestream Molecular Imaging Software for the First Time later in this chapter If you have purchased the Carestream Molecular Imaging Software Regulatory Edition with Network Licensing Carestream MI RE to use with your GL212
17. iadaaa 4 9 Updating Custom User Settings ereire es reete emen e eee hee 4 10 Deleting Custom User Settings esee seh ee ents ele mh rmt 4 10 Using the Focusing Tool ssseeeeeeeeeeee eee 4 11 Applying an Illumination Reference 0 0 0 0 eese 4 12 Adding an Annotation Bar to the Printed Image 0004 4 13 Using the Transilluminator for Non Imaging Applications 4 14 Maintaining Your System 16 0 00 ore ahhh hmc mcm 5 1 Maintaining Your Carestream GL212 PRO Imaging Cabinet 5 2 Cleaning the Cabinet ioter eR nt EE EE E WE d ebrei esE Y 5 2 Cleaning Filt ts 2 22 ep EEUU RE ERER MBPS PERI DR 5 3 Cleaning the White Black Epi illumination Pads lesse lessen 5 3 Repairing Your Carestream Gel Logic 212 PRO Imaging System 5 4 Replacing the UV Transilluminator Bulbs 0 0 0 0 cece eee eee 5 4 Replacing the White Light Epi Illumination Bulbs 00 005 5 6 Replacing the UV Epi Illumination Module Bulbs Optional Accessory 5 8 Replacing the UV Transilluminator Platen llle eese 5 10 Troubleshooting the System L sun 6 1 Technical Support i iusti be ake Shae PS PR Aedes RE RN 6 2 Carestream Molecular Imaging Technical Support 0 000000000 6 2 Common Instrument Problems 00 00 02 cece eee eee eee 6 3 Common Image Problems 05 0 2 46408 sare
18. image capture E The Lens section contains the FOV Aperture and Focus settings The FOV buttons adjusts the field of view The Aperture regulates the amount of light reaching lens for an exposure The Focus enables you to adjust focus for vary ing sample types F The llumination sections contains the pop up menu in which you select your illumination type White light epi illumination and UV transillumination are provided as standard illumination modes White light transillumination and UV epi illumination modules are available as optional accessories G The Emission Filter section selects the filter you wish to use A 590 nm filter is included in the package It is especially designed to eliminate light other than 590 nm wavelength produced by ethidium bromide or other similar emission wavelength Additional filters including 440 nm 535 nm 570 nm and 670 nm are available from Carestream Molecular Imaging or your Carestream Molec ular Imaging dealer See Appendix A Parts and Accessories dp lt z 6 O c E lt 6 2 7 The GL212 PRO Image Preview When the GL212 PRO dialog box is open the preview initiates the live stream of single capture images in the Image Preview window The Progress bar become active and provide feedback on the current capture The Preview ON OFF is a toggle switch Preview is used primarily for optimizing your capture conditions including sample placement aperture f
19. power cord into the AC MAINS outlet 13 Turn on the cabinet power switch 5 7 5 lt gm 5 sj 3 e 5 8 Replacing the UV Epi illumination Module Bulbs Optional Accessory The transilluminator consists of two 2 bulbs We recommend replacing all bulbs when servicing the light source Tools required Y 2 5 mm Allen wrench Replacement parts V GL212UV Epi illuminator Bulb Kit 2 bulbs 1 Turn off the cabinet switch 2 Remove the power cord from the AC MAINS outlet MAINS outlet Always turn the switch the cabinet off and disconnect the unit from the AC MAINS outlet before performing any service 3 Open the GL212 PRO Imaging Cabinet door WARNING The power cord is the primary disconnect device from the AC 4 Unplug the power connector from the receptacle inside the cabinet above the door on the left side 5 Loosen the thumbscrews that are holding the Epi illumination Module in the cabinet door 6 Remove the Epi illumination Module from the door and place on a flat surface 7 Remove the four 4 screws from the bottom and sides of module 8 Remove the cover plate 9 Remove the bulb by rotating 90 degrees in either direction This allows the pins to be lifted out of the slots at the top of the bulb holder 10 Insert a new bulb into the slots and rotate the bulb until a click is heard which locks the bulb in place 11 Proceed with steps 9 and 10 above for the second bu
20. the image Type Recommended UV Transillumination 3 seconds White Light Epi Illumination 0 1 second White Light Transillumination 0 005 ES NOTE The aperture controls the amount of light that passes through the lens while the exposure time controls how long the CCD is exposed Improve resolution by using the smaller aperture with a longer exposure time Time 0 003 mu Type an exposure time in the Exposure Time text edit box Use the Auto Exposure button for automated exposure determination Q Use the Coarse and Fine Exposure arrows to adjust the o exposure time in either coarse or fine increments This eliminates the need for you to use your keyboard Fine o ES NOTE The minimum and maximum exposure times are 0 001 second per exposure and 16 seconds per exposure respectively The time units can be set as seconds or minutes The minimum increment is 0 001 seconds for short exposures Adjust the aperture using the Aperture buttons located Aperture o in the Lens section of the GL212 PRO Acquire window 4 Adjust the F O V buttons to fill the entire frame with the sample image or features of interest This maximizes the number of pixels within your sample image rv O wer NOTE The F 0 V setting relates directly to the image resolution The GL212 PRO CCD camera has a pixel resolution of 1280 x 960 pixels At the smallest field of view 48 mm the number of pixels a
21. 4 11 FOV 2 13 4 7 Launching Carestream MI Software 2 6 3 13 3 15 3 16 3 22 4 2 M Macintosh Requirements 1 5 Software installation 3 8 3 12 maintenance 5 1 5 10 Molecular Imaging Software Carestream installation 3 3 3 12 launching2 6 3 13 3 15 3 16 3 22 4 2 Macintosh install 3 8 3 12 Preferences 2 16 2 19 Package contents 1 4 System requirements 1 5 Windows 3 3 3 7 multiple exposures 2 1 1 2 12 4 8 P Package Contents 1 4 Preferences image capture 2 16 2 19 Preview 2 6 2 16 2 19 4 5 4 8 Q Quick Print 2 6 2 13 R reference file applying 2 6 2 14 4 12 registering 1 7 regulatory information W 6 repacking W 5 repairing 5 4 5 10 epi white light bulbs 5 6 5 7 epi UV light bulbs 5 8 5 9 UV transilluminator 5 4 5 5 UV Transilluminator Platen 5 10 requirements electrical 3 2 environmental 3 2 space 3 2 system 1 5 S sample orienting 2 16 2 17 saturation 2 1 1 2 12 2 19 4 6 4 7 Saving settings 2 9 2 10 2 16 2 19 4 9 4 10 single exposure 2 11 2 12 4 7 software installation 3 3 3 12 launching2 6 3 13 3 15 3 16 3 22 4 2 Macintosh install 3 8 3 12 Preferences 2 16 2 19 Package contents 1 4 System requirements 1 5 Windows 3 3 3 7 space requirements 2 2 standard capture 2 11 2 12 4 7 Stop Preview button 2 6 2 16 2 19 4 5 4 8 SYBR Green 3 31 4 4 SYBR Gold 3 31 4 4 SYPRO Orange 3 31 4 4 System GL212 PRO component
22. Accessories are available for use with your Carestream Gel Logic 212 PRO Imaging System to expand your system s capabilities v The White Light Transillumination Module optional accessory provides white light transillumination that is ideal for imaging translucent samples e g Coomassie stained gels or plate assays dn bunes OD v The UV Epi illumination Light Modules are optional accessory The light accessory is easily installed inside the GL212 PRO Imaging Cabinet door Wavelengths available include 370 nm 306 nm or 254 nm v The UV Safety Shield optional accessory is designed to protect you from UV when using the UV transilluminator for non imaging applications such as viewing gels or cutting bands Installing the White Light Transilluminator Module Optional Accessory The White Light Transilluminator Module is an optional accessory The light accessory is easily installed inside the GL212 PRO Imaging Cabinet 1 Unpack the module and carefully examine it to determine that the imaging surface is not broken or scratched 2 Open the cabinet door 3 Insert the White Light Transilluminator Module into the cabinet engaging the tabs into the retaining clips mounted in the back of the cabinet 3 23 WARNING Cover the Platen with a soft cloth to protect it during the A installation 4 Connect the White Light Transilluminator Connector the port located on the right side of the cabinet ceiling 5 Use the
23. PRO v Follow the instructions in the Carestream Molecular Imaging Software Regulatory Edition Network Administrator s Manual to install Carestream MI RE Software v Then proceed to Launching Carestream Molecular Imaging Software for the First Time later in this chapter Uninstalling a Previous Version of MI or 1D Software Macintosh Remove your MI or 1D copy protection device from your computer 2 Inactivate any virus protection software ES NOTE Norton Utilities and Norton Antivirus software must be deactivated before you uninstall the software The software might not uninstall properly with the virus protection left running After installation you can restart your virus protection software 3 p t ot o 3 Close all software applications that may be running on your computer ES NOTE Cameras should not be connected to the computer while uninstalling the software 4 Move any customized standards or templates and any projects from their respective subfolders in your existing MI X X or ID X X folder to a temporary folder outside the folder If you are currently using the MI database also move the database folder Click the Applications folder and locate the database folder labeled GMPDB located in the MI folder and the KodakAdminPortal subfolder 5 Place the MI X X or 1D X X folder in the Trash ES NOTE If uninstalling MI version 4 5 or greater double click the uninstall applicat
24. S DURING PRODUCT SHIPMENT THIS WARRANTY DOES NOT COVER CIRCUMSTANCES BEYOND CARESTRAM HEALTH S CONTROL SERVICE OR PARTS TO CORRECT PROBLEMS RESULTING FROM THE USE OF ATTACHMENTS ACCESSORIES OR ALTERATIONS NOT MARKETED BY CARESTREAM HEALTH INC SERVICE REQUIRED AS THE RESULT OF UNAUTHORIZED MODIFICATIONS OR SERVICE MISUSE ABUSE FAILURE TO FOLLOW CARESTREAM HEALTH S OPERATING MAINTENANCE OR REPACKAGING INSTRUCTIONS OR FAILURE TO USE ITEMS SUPPLIED BY CARESTREAM HEALTH INC SUCH AS ADAPTERS AND CABLES CARESTREAM HEALTH INC MAKES NO OTHER WARRANTIES EXPRESS IMPLIED OR OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE FOR THIS EQUIPMENT OR SOFTWARE REPAIR OR REPLACEMENT WITHOUT CHARGE ARE CARESTREAM HEALTH S ONLY OBLIGATION UNDER THIS WARRANTY CARESTREAM HEALTH INC WILL NOT BE RESPONSIBLE FOR ANY SPECIAL CONSEQUENTIAL OR INCIDENTAL DAMAGES RESULTING FROM THE PURCHASE USE OR IMPROPER FUNCTIONING OF THIS EQUIPMENT REGARDLESS OF THE CAUSE SUCH DAMAGES FOR WHICH CARESTREAM HEALTH INC WILL NOT BE RESPONSIBLE INCLUDE BUT ARE NOT LIMITED TO LOSS OF REVENUE OR PROFIT DOWNTIME COSTS LOSS OF USE OF THE EQUIPMENT COST OF ANY SUBSTITUTE EQUIPMENT FACILITIES OR SERVICES OR CLAIMS OF YOUR CUSTOMERS FOR SUCH DAMAGES Depending on your geographical location some limitations and exclusions may not apply How to Obtain Service If the instrument does not function properly during the warranty period contact Car
25. TE If no shortcut is found navigate to Programs from the Start menu and selecting Carestream MI SE from the Carestream Molecular Imaging menu Windows or by clicking on the Carestream MI icon found in the Carestream Molecular Imaging folder Macintosh 2 Choose Select Digital Camera from the File menu Choose the GL212 PRO from the Digital Camera pop up menu The software systematically searches the appropriate computer port s If the selected imaging device is not found one of several messages appear on screen depending upon the imaging system selected Select Digital Camera Digital Camera eunt 2PRO Y Port Connection Speed 3 Click OK ES NOTE If you fail to communicate and have followed the suggestions in the dialog box please contact Carestream Molecular Imaging Technical Support for further assistance GL212PRO It was not possible to establish a connection to the GL212PRO Please check the Following 1 Is the GL212PRO plugged in and turned on The green power light should be on dn uias Q3 2 Have you connected the communications cable from the back of the GL212PRO to a serial communications port on this comupter 3 Have you connected the Firewire cable from the back of the GL212PRO to a Firewire port on this computer 4 Is your camera system a different model and not a GL212PRO Look for a label on the front of the unit If you are still not able to establish a connection after checki
26. age with a different filter selected Contaminated optics Contact Carestream Molecular Imaging Technical Support Image appears with many speckles in a UV illuminated image Dust or dirt on the Platen See Preparing for Imaging in Chapter 4 Capturing Images Image partially blocked Filter not centered over the lens Check that the filter is lined up properly properly Lines on the image Scratched Platen Contact Carestream Molecular Imaging Technical Support for further instructions The background intensity of the image is uneven in the Project window Improper Illumination Reference File was used for correction Be sure the proper Illumination Reference File is used See Applying an Illumination Correction in Chapter 4 Capturing Images Bright imaging artifacts not coming from the sample Light leak Ensure that the door is securely closed e e iex 0 n pop 9 9 cu Ce Problem Probable Cause Solution Sample is not visible Sample not in field of view Appropriate Illumination source not selected Wrong filter is being used Adjust FOV to find sample To aid in positioning use the White Epi Illumination source while previewing Select the correct illumination source in the GL212 PRO Acquire window Check filter Insufficient sample loaded Increase sample loaded Sample overstained EtBr
27. allation is occurring complete your Carestream MI Software Registration Card and return the card by mail This process takes only a few minutes to complete and ensures that you receive information regarding new software releases periodic maintenance releases and technical bulletins 6 The installer notifies you when the installation is complete Click Restart T Proceed to Copy Protection Device Installation Macintosh Copy Protection Device Installation Macintosh Carestream Molecular Imaging Software is copy protected using a device that plugs into the USB port of your computer Carestream MI will not launch unless this device is attached to your computer If you are installing the system for the first time locate and install the copy protection device according to the instructions below If you are upgrading your system your package may or may not contain a new copy protection device To install the copy protection device 3 CD F Ke c Ke 1 Plug the copy protection device into a USB port of your computer Please make sure that the connection is secure ES NOTE If you are upgrading from a previous version of the MI or 1D Software and received a new copy protection device you must attach both the old and new copy protection devices to your computer After you launch the newest version for the first time your old copy protection device is deactivated Remove and discard the old key 2 Proceed to Macinto
28. allation of Carestream MI comes with a single user with the User Name Admin and the Password password Use this User name to gain access to Carestream MI Image Database to set up additional users 1 Select Database from the Navigation panel 2 Your web browser launches and displays the Image Database window opens Carestream Molecular Imaging Software 3 Select the default Username Admin type the Password password No quotation marks and click Login 4 Click the Users tab to access the User Administration page where you can add users Select Add Users arestream Image Database 3 CD F Ke c T 5 The User Information page appears Fill out the information including First Name Last Name Username enter a temporary password Password Job Function Administrator Principal Investigator Research Scientist an e mail address Click Create E3 NOTE New users are automatically added to the default Investigation and default Study 6 You may want to take some time to click on the Server Setup button to give your database server a name and specify a SMTP mail server arestream Image Database 3 17 3 18 v Server ID is a unique name for your machine The Server ID can either be a name or number Y Administrator E mail this indicates the e mail address of the Administrators s Y Days until password expire allows administrators to determine how freq
29. allergy and allergic reactions Medications or cosmetics may increase your sensitivity to ultraviolet radiation Consult physician before operating this product if you are using medications or have a history of skin problems or believe yourself especially sensitive to sunlight For Laboratory Use Only DANGER Ultraviolet radiation emitted from this product Avoid exposure FEN 3 p t ot o The UV source does not operate when the door is open Do not attempt to override this feature Discontinue use if the Platen is damaged or broken Open the front door of the GL212 PRO Imaging Cabinet 2 Use the handle to pull out the UV transilluminator until it stops 3 Fully insert the lower edge of the safety shield in the slots in the two 2 black support brackets we NOTE The Gel Tray provides a cutting surface for excising bands from your gel when using the optional UV Safety Shield ES NOTE The UV Transilluminator illuminates when UV Trans is selected in the GL212 PRO Acquire window and you press the switch on the front of the Transilluminator 4 If you do not have any other optional accessories proceed to Launching Carestream Molecular Imaging Software with your Carestream Gel Logic 212 PRO System 3 27 3 28 Launching Carestream Molecular Imaging Software with your Carestream Gel Logic 212 PRO Imaging System 1 Launch the software by clicking on the desktop application icon shortcut E3 NO
30. als contaminated with radiological substances decontaminate with a radioactive decontamination detergent following manufacturer s instructions and your institutional safety protocols for decontaminating and handling radioactive waste WARNING Never use harsh or abrasive cleaners or organic solvents on the any parts of the system WARNING When handling or using ammonia based spray cleaners follow instructions noted in their Material Safety Data Sheet MSDS WARNING When cleaning your GL212 PRO we recommend that you wear impervious gloves e g latex or nitrile v To dry use a soft cloth paper towel or allow to air dry Cleaning Filters Open the cabinet door 2 Select Preferences from the GL212 PRO Acquire window The Preferences window appears 3 Click the Emission Filter Change button The Edit Filters dialog box opens 4 Click Load button for the filter you want to remove to clean 5 Using the Filter Tool provided with your instrument insert the filter in the filter wheel with the open end of the tool 6 Retrieve the filter by pressing firmly on the handle of the tool 7 Continue pressing as you lift the filter out of the filter wheel and release your grip as you gently place the filter onto a flat surface 8 Wipe gently with a soft lint free cloth or an untreated lens cleaning tissue E WARNING Do not use cleaning solutions unless they are designed specifically for camera lenses Do not wi
31. ance v To keep from damaging your GL212 PRO Camera Assembly avoid exposure to moisture and extreme temperatures MAINS outlet Always turn the main power switch off and disconnect the unit WARNING The power cord is the primary disconnect device from the AC from the AC MAINS outlet before performing any service A WARNING When handling or using ammonia based spray cleaner follow instructions noted in the Material Safety Data Sheet MSDS A WARNING When cleaning your GL212 PRO we recommend that you wear impervious gloves e g latex or nitrile 5 1 5 lt E 5 B 3 5 2 Maintaining Your Carestream Gel Logic 212 PRO Imaging Cabinet The GL212 PRO Imaging Cabinet is fabricated to facilitate easy cleaning We recommend that you clean your cabinet and accessories regularly to keep them in good working order WARNING The power cord is the primary disconnect device from the AC MAINS supply Always turn the switch the cabinet off and disconnect the unit from the AC MAINS outlet before performing any maintenance Cleaning the Cabinet The cabinet is designed for easy care v For material not contaminated with biological or radiological substances wipe with a non abrasive cleaner v For materials contaminated with biological substances prepare a 5 bleach solution in water Wipe down the cabinet with the bleach solution using a clean cloth or sponge Wipe clean with water WY For materi
32. ation place the sample on the black side of the Epi White Black Backdrop Screen positioned in the Gel Tray ES NOTE Special care should be taken if your sample is dry Ensure a dry surface by a quick ethanol wipe amp NOTE Do not use metal utensils or tweezers since they may scratch and permanently damage the glass surface 3 Choose an optical filter appropriate for your imaging type using the Emission Filter pop up menu Refer to the Filter Selection Table below Dye Stain Filter nm Coomassie Blue 590 or none Ethidium Bromide 590 Fluorescein 535 Oregon Green 535 Gel Star 535 SYPRO Orange 590 SYBR Green 535 SYBR Gold 535 Previewing and Capturing Images The Preview mode offers you a way to view what the camera will image In preview mode you can optimize the sample placement aperture field of view and exposure time Launching the Acquire window initiates the live stream of single capture images The Progress bars are active providing feedback on the current capture 1 Once the sample is in place close the GL212 PRO Imaging Cabinet door The Preview is initiated If the sample is not being displayed click Preview Gel Logic 212 PRO a o s enne Illumination Settings Current Session X User Name x Annotation Exposure I oan 90 S pe et Z 5 Ke 3 D on ue NOTE Preview is used primarily for optimizing
33. be disabled because these are not user defined for FireWire devices WY Once a GL212 PRO is found the Status bar and the Camera Access button reflect the current camera selection 4 2 4 The GL212 PRO Acquire window appears Gel Logic 212 PRO Capture Settings Current Session j User Name z 5 You are ready to begin imaging Proceed to Preparing for Image Capture S O e EL 5 Ke 3 D Q D 0 4 3 4 4 Preparing for Image Capture Your GL212 PRO should be installed if it is has not yet been installed see Chapter 3 Setting up the System Be sure that the imaging surface is clean prior to any sample placement Dust particles or scratches on the surface may introduce artifacts in your data Extra care should be taken when cleaning the surface to avoid scratches 1 Open the GL212 PRO Imaging Cabinet Door 2 Place the sample in the center of the appropriate imaging surface v For white light transillumination using the optional accessory place the sample on the fold down White Light Transilluminator Module inside the cabinet v For UV transillumination place the sample on the UV Transilluminator Platen v White light epi illumination place the sample on the Epi White Black Backdrop Screen positioned in the Gel Tray Place the sample on the black or white side to achieve the best contrast i e Coomassie stained gels are best imaged on the white side v UV epi illumin
34. binet has been designed to maintain the proper focal distance from the camera to your sample to minimize the need to focus If you should you need to adjust the focus use the Focus buttons If your samples vary in thickness the software assisted focusing tool is available for use The software determines camera focus by using the sharpness of edges within the image To adjust focus 1 2 3 4 5 6 Place the focus target or your sample in the GL212 PRO Imaging Cabinet Select the Focus checkbox Adjust the exposure time and f stop to best represent your image in the Preview Adjust the F O V to fill the field of view with the focus target or your sample Click the Focus Camera checkbox A selection rectangle appears on screen in the center of the image Adjust the Focus ring to maximize the numerical value appearing in the text box v Increasing values indicate focus is sharpening whereas decreasing values indicate blurring of the image V A green or red indicator light provides a visual indicator As you are improving focus the indicator light signals green Moving the focus in the wrong direction the indicator light signals red To exit the Focusing mode uncheck the Focus Camera i9 S pe r c ef 5 Ke 3 D on Applying an Illumination Reference Improve the quality of your data by applying field illumination correction to images captured The illumination non uniformity is highly repr
35. ctions we IMPORTANT Settings will NOT save the Aperture Field of View Focus or User Name or Annotations Saving Custom User Settings 1 Make all your selections from within the GL212 PRO Acquire window 2 Choose the Save Settings from the Settings pop up menu A File Name dialog box appears 3 Enter a name to describe your custom settings i9 xe Lu E 5 e 3 D oj D o 4 Click OK The settings are saved and will appear in the list of available settings ES NOTE If you choose a custom acquire setting and then make modifications to the custom settings they will not be saved unless you update the custom setting by selecting Update Settings from the Settings pop up menu ES NOTE Custom settings are saved as individual files in C Program Files Molecular Imaging MIApplicationGL 212 XGL 212 Settings Windows or in the Molecular Imaging MIA pplicationMI Extensions GL 212 Settings Macintosh subfolders 4 9 4 10 Updating Custom User Settings 1 Select a custom setting from the Settings pop up menu 2 Modify settings as desired 3 Choose Update Settings in the Settings pop up menu E3 NOTE The GL212 PRO factory defaults cannot be updated Deleting Custom User Settings 1 Select a custom setting from the Settings pop up menu 2 Choose Delete Settings Ex NOTE The GL212 PRO factory defaults cannot be deleted Using the Focusing Tool The GL212 PRO Imaging Ca
36. ctions Medications or cosmetics may increase your sensitivity to ultraviolet radiation Consult physician before operating this product if you are using medications or have a history of skin problems or believe yourself especially sensitive to sunlight For Laboratory Use Only DANGER Ultraviolet radiation emitted from this product Avoid exposure A The Safety Shield must be in place when the door is open and the UV Transilluminator is activated UV will not be emitted at the Platen surface if the UV Safety Shield is not in place Replace the UV Safety Shield if cracked or damaged Personal Protective Wear If your hands are in close contact with the Platen surface or anywhere behind the UV Safety Shield to prevent injury it is imperative that appropriate gloves be worn i e nitrile Latex gloves are also effective however latex may present an allergic reaction in some individuals In addition long sleeved lab coat or other tightly woven material will be effective in reducing UV exposures Protective eye wear should be worn by users when the Platen surface is pulled out 4 When finished remove the UV Safety Shield to disengage the UV transilluminator and turn off UV illumination 5 Remove the sample from the cabinet or proceed with an imaging protocol 4 14 Maintaining Your System Your Carestream Gel Logic 212 PRO Imaging System GL212 PRO is a delicate piece of photographic equipment requiring care and mainten
37. d uninstall features on some systems may not remove all previous program elements Check drives for residual folders and files Manual deletion of these folders and files may be necessary Restart your computer Proceed to Carestream Molecular Imaging Software Installation Windows Carestream Molecular Imaging Software Installation Windows Carestream Molecular Imaging Software is installed like most Windows application programs and requires administrator privileges 1 Inactivate any virus protection software ES NOTE Norton Utilities and Norton Antivirus software must be deactivated before you install the software The installation might not run properly with the virus protection left running After installation you can restart your virus protection software Close all software applications that may be running on your computer E3 NOTE Cameras should not be connected to the computer while installing the software ES NOTE Remove any copy protection devices attached to your computer during installation Insert the Carestream Molecular Imaging Software Version 5 X CD into your CD drive and double click on the Carestream MI SE Installer exe icon to launch the installer Ex NOTE To install Carestream MI 5 X on Windows Vista right click on the Installer and select Run as Administrator In the User Account Control pop up menu select All to obtain administrator privileges to allow the installer to run Th
38. drawer out far enough to allow access to the four 4 Phillips head screws on the sides of the Platen top Remove the four 4 screws that attach the Platen top Lift the Platen from the UV Transilluminator and carefully set it aside Position the new Platen on the transilluminator base and reinstall the four 4 screws Plug the power cord into the AC MAINS outlet Turn on the cabinet power switch Troubleshooting the System In this chapter common questions are addressed The questions are divided into three sections Instrument Image and Software If you still have questions after reading this section and the corresponding information in the User s Guide contact Carestream Molecular Imaging Technical Support Please have your serial number and any technical information available fe c O D a 5 O o ch 5 Ke 6 1 6 2 Technical Support For technical support contact Carestream Molecular Imaging Technical Support or your Carestream Molecular Imaging dealer For up to date dealer information visit our WEB site at mi carestreamhealth com When contacting technical support please have the following information available w The serial number of your GL212 PRO located on the back of the unit w The serial number of your GL212 PRO Camera press Control and T buttons simultaneously when the GL212 PRO Acquire window is displayed UY The serial number and version number of your Carestream MI
39. e capture the exposure time and the illumination source The text appears on the long axis at the top of the page All items in the Annotation bar are separate objects and can be displayed in the Annotations window This allows you to edit the text change the font style color or size prior to printing The contents are grouped therefore you must ungroup these objects prior to editing pee NOTE Each time you capture an image the Annotations text edit box is cleared Annotations are not saved with Custom Settings E3 NOTE Display or hide the Annotation bar by selecting the Show Annotation bar from within the Annotations panel Exposure Section of the GL212 PRO Acquire Window You can define the exposure parameters using the Exposure section of the GL212 PRO Acquire window Select the type of exposure exposure time and number of exposures Exposure Type Single Time 0 003 aa ae o No Exposures z Expt Duration 0 003 sec Fine o OQ The Exposure Type Options Type Single Single Multiple Time Lapse The Type pop up menu defines the type of exposure You can choose to take a single multiple or time lapse exposure Using these exposure modes the minimum and maximum exposure times are 0 001 second and 16 seconds respectively v Single Exposure takes one 12 bit image After capture the image file automatically opens as an Carestream MI project v Multiple Exposure Final Accu
40. e installer leads you through the installation process Make sure to select the Complete Setup to install the software in the default directory to ensure full functionality of the system ES NOTE The Select Components window allows you to install Carestream MI without the database Only install the database in the location that you want to maintain images acquired from the system While the software installation is occurring complete your Carestream MI Software Registration Card and return the card by mail This ensures that you receive information on new software releases periodic maintenance releases and technical bulletins A dialog box appears when the installation is complete Select the option to restart the computer and click Finish Proceed to Copy Protection Device Installation Windows 3 5 3 p t ot o 3 6 Copy Protection Device Installation Windows Carestream MI is copy protected using a device that plugs into the USB port of your computer It will not launch unless this device is attached to your computer If you are installing the system for the first time locate and install the copy protection device according to the instructions below If you are upgrading your system your package may or may not contain a new copy protection device To install the copy protection device 1 Plug the copy protection device into a USB port of your computer Please make sure that the connection i
41. e sample work area is 35 cm by 47 cm how ever the UV image area is limited to 24 5 cm x 33 cm DANGER Ultraviolet radiation emitted from this product Avoid exposure AAN ALWAYS WEAR PROTECTIVE CLOTHING EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER ALWAYS WEAR PROTECTIVE EYEWEAR FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURY TO EYE Never look directly into the lamp Exposure can cause eye and skin allergy and allergic reactions Medications or cosmetics may increase your sensitivity to ultraviolet radiation Consult physician before operating this product if you are using medications or have a history of skin problems or believe yourself especially sensitive to sunlight For Laboratory Use Only The UV source does not operate when the door is open Do not attempt to override this feature wm lt o 6 O lt S S 6 Discontinue use if the Platen is damaged or broken G The UV Switch turns on the UV transilluminator only when the optional UV Safety Shield is in place and UV Trans is selected in the GL212 PRO Acquire window Use this feature to visualize or excise bands 2 3 2 4 H The UV Safety Shield not shown optional accessory is designed to limit your exposure to UV when using the UV transilluminator for non imaging applica tions such as viewing gels or cutting bands ES NOTE The optional UV Safety Shield engages an inter lock feature and allows A safe vie
42. e window 2 7 2 20 4 2 4 3 4 8 4 16 Buttons section 2 6 2 13 Capture section 2 6 2 9 2 10 Emission Filter section 2 2 2 6 2 17 3 30 3 31 4 4 Exposure section 2 6 2 11 2 12 Illumination section 2 6 2 14 4 5 4 6 Lens section 2 6 2 13 Preferences section 2 6 2 16 2 19 Preview 2 8 Annotations bar 2 6 2 10 4 13 Aperture 2 13 4 7 C Cabinet Imaging installation 3 20 3 33 overview2 2 2 5 Cable Connector Camera 2 2 2 3 3 21 3 22 camera about 1 1 2 2 2 5 computer interface cable 2 2 2 3 3 21 3 22 installation 3 20 3 22 Camera Cable Connector 2 2 2 3 3 21 3 22 Capture Annotation bar 4 13 Custom settings 2 16 2 19 4 0 4 10 Focusing Tool 4 11 Illumination Reference 4 12 images 4 1 4 14 Launching software 4 2 Preparing 4 4 4 5 Previewing 4 5 4 8 Charged Coupled Device CCD 1 1 2 2 3 1 cleaning Cabinet 5 2 Epi illumination pads 5 3 Filters 5 3 conventions user s guide 1 2 Coomasse Blue 3 31 4 4 copy protection device 3 3 3 6 3 9 3 11 correction illumination 2 14 4 12 Custom Capture settings 2 16 2 19 4 9 4 10 D deleting setting 4 10 digital camera 2 2 2 3 X pu selecting 2 6 3 28 3 29 4 2 decontamination W4 W 5 E electrical Requirements 2 2 Emission filters 2 2 2 17 3 30 3 31 4 4 environmental Requirements 2 2 epi illumination installing 3 20 3 22 3 25 3 26 overview 2 3 2 4 2 14 4 4 using 4 5 4 8
43. el facilitates the use of up to six 6 filters A 590 nm filter is included in the package Additional filters including 440 nm 535 nm 570 nm and 670 nm are available from Carestream Molecular Imaging or your Carestream Molecular Imaging dealer See Appendix A Parts and Accesso ries D The maging Cabinet Door accesses the area under the camera which accom modates the imaging of samples up to 24 5 x 33 cm E The UV Epi illumination Light is an optional accessory The light accessory is easily installed inside the GL212 PRO cabinet door Wavelengths available include 370 nm 306 nm or 254 nm DANGER Ultraviolet radiation emitted from this product Avoid exposure Ler ALWAYS WEAR PROTECTIVE CLOTHING EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER ALWAYS WEAR PROTECTIVE EYEWEAR FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURY TO EYE Never look directly into the lamp Exposure can cause eye and skin allergy and allergic reactions Medications or cosmetics may increase your sensitivity to ultraviolet radiation Consult physician before operating this product if you are using medications or have a history of skin problems or believe yourself especially sensitive to sunlight For Laboratory Use Only The UV source does not operate when the door is open Do not attempt to override this feature F The UV Transilluminator provides a uniform intense 306 nm peak emission source of ultraviolet radiation Th
44. er 590 z Timelapse gt Gy iaculi Quick Print 0 100 Sec Coane Q 10 000 Sec Preferences 2 00 EE 7 Click and drag to position the rectangle to contain the center of the focus target and then choose Auto Focus or adjust the Focal Plane using the buttons ES NOTE Hold down the Escape key if you find that the fine focus has passed through the best focus value Auto Focus will use the best focus value up to the time you pressed the Auto Focus button again 8 You are now ready to begin capturing images To learn about capturing images with the GL212 PRO proceed to Chapter 4 Capturing Images 3 CD F c Ke 3 33 3 34 Capturing Images Once your Carestream Gel Logic 212 PRO Imaging System GL212 PRO has been set up and Carestream Molecular Imaging Software Carestream MI is installed you are ready to capture high quality digital images The GL212 PRO supports several image capture methods including single capture exposure multiple capture exposure accumulated into a single file or as separate files and time lapse exposures Using these exposure modes the minimum and maximum exposure times are 0 001 second and 16 seconds respectively v Using GL212 PRO you can document your laboratory experiments with floating point accuracy and analyze images DNA RNA and protein electrophoresis gels including gels stained with ethidium bromide SYPRO Orange SYBR
45. er s Guide is divided into the chapters listed below Y Chapter 1 Introduction gets you started by describing system components and requirements WY Chapter 2 System Overview details the instrument and software related to GL212 PRO Y Chapter 3 Setting Up Your System walks you through the installation process v Chapter 4 Capturing Images describes how to use the GL212 PRO to capture images v Chapter 5 Maintaining Your System describes how to care for and maintain your GL212 PRO v Chapter 6 Troubleshooting offers tips if you encounter any problems with your system al v Warranty reviews the warranty and repair coverage provided by Carestream Health Inc v Appendix A Related Products provides you information on accessories and replacement parts for your GL212 PRO S x 9 Q en 2 ct o 1 3 1 4 Package Contents When unpacking your Carestream Gel Logic 212 PRO Imaging System please take a moment to ensure that all the necessary parts have been received Your package should contain Carestream Gel Logic 212 PRO Hardware 1 Y Carestream GL212 PRO Imaging Cabinet with the Carestream GL212 PRO Camera v GL PRO Camera Computer Interface FireWire Cable v Serial Communication Cable v 590 nm Filter 52 mm v Filter Tool Y Carestream GL212 PRO User s Guide v White Black Epi Illumination Pad Set v Gel Tray v UV Shield optional accessory v UV Epi illumination Modules 370
46. es image acquisition Once acquired files can be analyzed annotated and printed The Quick Print button sends the image directly to your Quick Print printer and no copy of the image is saved Preferences The Preferences button opens the GL212 PRO Preferences window is where you can customize certain Doe capture parameters The GL212 PRO Preferences window is described in more detail later in this chapter The Done button closes the GL212 PRO Acquire window and returns you to Carestream MI Lens Section of the GL212 PRO Acquire Window The GL212 PRO camera lens is computer controlled wO O m O O rO O The Field of View F O V buttons adjusts the field of view setting of the lens The Aperture buttons regulates the amount of light reaching the sensor for an exposure The Focus buttons adjusts the focus of the lens The Focus checkbox accesses the software assisted focusing aid that is described in Using the Focusing Tool in Chapter 4 Capturing Images wm lt o 6 O c E lt 6 Z 2 13 2 14 Illumination Section of the GL212 PRO Acquire Window After selecting the type of exposures you want to take from the Type pop up menu the exposure setting section updates with the appropriate text and checkboxes for that type of capture The GL212 PRO can image up to a 24 5 x 33 cm area Illumination wm 10 Apply Correction Illumination Pop Up Menu White Lig
47. estream MI and your computer Proceed to Installing the Carestream Gel Logic 212 PRO Imaging System 3 19 3 20 Installing the Carestream Gel Logic 212 PRO Imaging System The GL212 PRO has been designed to eliminate the need to take pictures in a darkroom The set up is designed to only take a few minutes So let s begin WARNING Do not plug the Camera Computer Interface Cable or the Communication Cable into the computer unless Carestream Molecular Imaging Software has been installed See Installing the Software Windows or Installing the Software Macintosh later in this chapter WARNING The power cord is the primary disconnect device from the AC MAINS outlet Do not connect the unit to the AC MAINS outlet until installation is complete Installing the GL212 PRO Imaging Hardware 1 Remove the base GL212 PRO Imaging Cabinet and GL212 Camera Module from the shipping container Remove packing materials Verify components listed in Package Contents in Chapter 1 Introduction of this User s Guide Position the base cabinet next to your computer Use the wrench provided to level the unit on your lab bench Verify that the cabinet door clears the table Open the door of the GL212 PRO Imaging Cabinet Carefully set the GL212 PRO Camera Module on top of the imaging cabinet Secure the GL212 PRO Camera Module to the cabinet by tightening the four 4 thumbscrews installed on the ceiling of the cabinet tw
48. estream Molecular Imaging Technical Support or your local dealer to arrange for service If your unit needs to be returned for any reason please contact your local dealer to obtain a return authorization All returned units must be decontaminated prior to their return as described below No returns will be accepted without a return authorization and proper decontamination documentation When contacting technical support please have the following information available w The serial number of your GL212 PRO located on the back of the unit v The serial number and version number of your Carestream MI Ex NOTE With the software running select About MI under the Help menu Windows or select About MI under the Apple menu item Macintosh w The type of computer you are using make model v Operating system software version Ex NOTE Check your operating system version by right clicking on the My Computer icon and then on Properties Windows or select About This Mac under the Apple menu item Macintosh 3 o gt m lt VU The type of image you are capturing or analyzing Y The problem you are having and what you were doing when the problem occurred Please note the exact wording of any error messages including any error numbers displayed Contact Carestream Molecular Imaging Technical Support by Y Utilizing our World Wide Web support pages at mi carestreamhealth com Y Calling Carestream Molecular Imaging Tech
49. ethidium bromide 3 31 4 4 Exposure 2 6 2 1 1 2 12 4 5 4 8 Multiple 2 11 2 12 4 8 Single 2 11 2 12 4 7 Standard 2 11 2 12 4 7 Time Lapse 2 1 1 2 12 4 8 F File capture 1 1 2 1 Illumination Reference 2 6 2 14 4 12 filter 440 nm2 2 2 17 3 30 3 31 4 4 535 nm 2 2 2 17 3 30 3 31 4 4 570 nm 2 2 2 17 3 30 3 31 4 4 670 nm 2 2 2 17 3 30 3 31 4 4 applying2 17 3 30 3 31 4 4 choosing 4 4 popup menu 2 2 2 17 3 30 3 31 4 4 wheel 2 2 2 17 3 30 3 31 4 4 FireWire camera cable connector 2 2 2 3 3 21 3 22 Focusing tool 4 11 H Hardware GL212 PRO electrical requirements 3 2 environmental requirements 3 2 overview 2 2 2 5 setup 3 20 3 31 space requirements 3 2 I IEEE 1394 port camera cable connector 2 2 2 3 3 21 3 22 Illumination choosing 2 14 4 5 4 6 Reference File 2 6 2 14 4 12 UV Epi 2 14 4 5 4 6 UV Trans 2 14 4 5 4 6 White Light Epi 2 4 2 5 4 6 Image area 2 3 4 4 4 7 capture 4 1 4 14 illumination correction 2 6 2 14 4 12 orientation 2 16 2 17 Preview 2 16 2 19 saturation 2 1 1 2 12 2 19 4 6 4 7 Imaging Cabinet about 2 2 2 5 setting up 3 20 3 33 Installation Carestream Molecular Imaging Software 3 3 3 19 hardware 3 20 3 33 UV Transilluminator 3 20 3 21 UV Epi Illumination Light 3 25 3 26 UV Safety Shield 3 27 White Light Transilluminator 3 20 Lens 2 13 Aperture 2 13 4 7 Focusing 2 13 4 7
50. fault orientation Rotate the image 90 counterclockwise Rotate the image 180 Rotate the image 90 clockwise s E e 2 16 Ex NOTE The Rotation buttons do not change the orientation of the preview image The rotation occurs during submission of the image to Carestream MI ES NOTE Rotation events are documented in the History tab of the Lane Information window since rotations are destructive to the original data file v Options You can choose to open images after capture in either Carestream MI Analysis panel or Annotations panel Open In Annotations du pen In Analysis NN E v Camera Delay configures the GL212 PRO camera to take a picture with 5 or 10 second delay This delay occurs after the capture command is executed and before the actual picture is taken Choose the time in the Camera Delay pop up menu UD _ 10 seconds E3 NOTE This option is useful in situations when light tables take several seconds to become completely illuminated WY Emission Filters Change button access the Edit Filters dialog box where you can load or change position of filters Edit Filters Filter Position A nm Label 4 Open Open Load1 440 440 Load 2 535 535 Load 3 so v a sx so geeads dp lt o 6 O lt S lt 6 eo so Ee on 2 17 2 18 White Light Transillumination Intensity Tran
51. g If the area still has a greater than 200 dpm cm continue the cycle of swipes and washing until you achieve a reading of less than 200 dpm cm Once the unit has been determined to be radiation free 200 dpm cm remove all the hazardous and radioactive labels from the unit If the labels cannot be removed deface them Failure to do so may result in significant delay or a refusal of repair If your unit has unremovable decontamination detectable with a GM meter and not with paper swipes or detectable with paper swipes but after continued washing the dpm cm remains constant above 200 of a short half life isotope such as p the unit may be stored for 10 half lives of isotopic decay and the decontamination procedure repeated ES NOTE Units contaminated with unremovable long half life isotopes may not be returned 3 o gt m x Repackaging the GL212 PRO Inappropriate packing will void the GL212 PRO warranty Follow these directions when packing the GL212 PRO for shipment 1 2 o oc n Decontaminate the GL212 PRO as described above Remove the power cord Camera Computer Cables and Serial communication from the rear of the GL212 PRO Remove the Filters optional White Light Transilluminator and or UV Epi illumination Modules from the imaging cabinet Unscrew and remove the Camera Assembly from the imaging cabinet Package the Camera Assembly in foam padding Place the components in their original plas
52. he back of the GL212 PRO and the computer The Camera Computer Interface Cable and Serial Cable that came with the system was not used Make sure that the cables being used is the one you received with your GL212 PRO No power to the camera Verify that the port is a 6 pin IEEE 1394 FireWire If you are using a laptop computer the 6 pin PMCIA Card Adaptor must be powered by an external power source No power to the system Verify that the Line Cord is connected to the AC MAINS and that the power switch is in the ON Position The green light on the front panel should be illuminated Wrong camera selected Verify that you selected GL212 PRO from the Digital Camera pop up menu in Digital Camera submenu of the File menu The on screen image is fractured or split into parts IEEE 1394 FireWire port malfunctioning the Add on FireWire card is improperly seated or the camera is defective Move the Camera Computer Interface Cable to another FireWire port if available Reseat Add on FireWire card UV Transilluminator lights are flickering Either the bulbs need replacement or a ballast problem Test whether the bulbs or the ballast needs replacement replace the UV bulbs with similarly sized white light bulbs If the bulbs flicker the ballast needs changing If the bulbs don t flicker the UV bulbs need replacement e c iex 0 2 pop 9 9 cu
53. he login windows and open to a Carestream MI Project window Proceed to Step 6 to enter your User Name Company and Carestream MI Software serial number 5 If you selected Login Required in the Carestream MI Security Setup dialog box you will be prompted to contact database administrator Click OK to open Carestream MI Future launches of Carestream MI will require both Username and Password to gain access to Carestream MI and the Carestream MI database 6 Type your Name and Organization in the boxes provided 3 14 T Enter your Serial Number exactly including dashes as provided on your registration card or serial number card included in your Carestream MI package ES NOTE The serial number is required when installing Carestream MI or when contacting Carestream Molecular Imaging Technical Support Keep the serial number in a safe location 8 Click OK The Carestream MI Project window appears Your installation is complete 3 CD F Ke c Ke E3 NOTE Previous MI or 1D users may move any customized standards or templates any projects and database folders back into the new MI folder ES NOTE If you selected Login Required in the Carestream MI Security Setup dialog box when Carestream MI was first launched you can set up User Names and Passwords for each Carestream MI user Proceed to Carestream MI Image Database later in this chapter 3 15 3 16 Carestream MI Image Database The initial inst
54. ht Trans Light Trans B White Light Trans using the White Light Transilluminator Module White Light Trans an optional accessory is ideal illumination for non opaque samples UV Trans plates and visible dye stained gels White Light Epi UV Epi UV Trans is ideal illumination for providing illumination of fluorescently stained images i e ethidium bromide SYBR Green White Light Epi is ideal illumination for imaging samples such as plates and blots UV Epi is ideal illumination for imaging samples like TLC plates This is an optional accessory Wavelengths available include 370 nm 306 nm and 254 nm Apply Correction Checkbox Apply Correction Use the Apply Correction checkbox to generate and apply an illumination correction To correct for uneven illumination and improve the quality of your data an illumination reference can be used The nonuniformity in the illumination generated by the UV bulbs or white light is highly reproducible and may be corrected by simply dividing the image by an illumination reference image The exposure settings used to capture the image must match those used to create an illumination reference image Please note that the illumination reference image cannot contain saturated pixels Emission Filter Section of the GL212 PRO Acquire Window Selecting a filter may optimize the fluorescent signal by discriminating against background and or differentiating between multiple fluoroch
55. ield of view FOV and exposure time While previewing a capture the focus options are accessible See Using the Focusing Tool in Chapter 4 Capturing Images Gel Logic 212 PRO Lens rov OQ 90 Focus Illumination Settings Current Session X White Light Trans User Name Y T Apply Correction Annotation Emission Filter E re 440 x Type Single c Quick Print Time 0 003 Sec ee o OQ maem No Exposures M Preferences aaa OO mum ES NOTE If you have selected a rotation preference the preview will not reflect the rotation setting The rotations are performed upon image capture and display after submission to Carestream MI The preview will continuously take and display images until you click the Capture or Quick Print button v The Capture button captures an image subject to the user exposure and zoom settings The image opens as a new Carestream MI project v The Quick Print button sends the captured image directly to the default printer without saving to disk 2 8 Capture Section of the GL212 PRO Acquire Window The Capture section of the GL212 PRO window contains the Settings User Name and Annotation options Let s review each option Capture Settings Current Session X Settings You can speed up the capture process by creating custom capture settings for the various types of experiments or for specific personal preferences Once saved these setti
56. imary disconnect device from the AC MAINS supply Always turn the Illumination Selector off and disconnect the unit from the AC MAINS source before performing any service I The White Light Epi illumination floods the chamber with light and is ideal for imaging opaque samples e g colorimetric stained blots or plate assays J The White Light Transillumination Module optional accessory provides white light transillumination that is ideal for imaging translucent samples e g Coomassie stained gels or plate assays K The Camera Cable Connector not shown couples the GL212 PRO camera to your computer s IEEE 1394 FireWire with a special Camera Computer Interface Cable This cable also provides power to the camera 1 amp 7 NOTE The GL212 PRO requires a 6 PIN IEEE 1394 FireWire connection If you are using a laptop computer with a PMCIA card the IEEE 1394 card must be powered by an external power source WARNING Use only the provided Camera Computer Interface Cable to attach A the GL212 PRO to your computer When plugging in the IEEE 1394 cable into the computer be sure to orient the camera cable connector properly with the narrow end pointing correctly to avoid damage to the circuitry and voiding the warranty WARNING We recommend connecting the GL212 PRO as the only device on the IEEE 1394 FireWire bus L The Serial Communication Cable Connector not shown couples the GL212 PRO Cabinet to your computer s serial
57. ion in the Applications folder and the MI subfolder Follow the on screen instructions to uninstall MI 6 Restart your computer 7 Empty the Trash 8 Proceed to Carestream Molecular Imaging Software Installation Macintosh 3 9 3 10 Carestream Molecular Imaging Software Installation Macintosh 1 Inactivate any virus protection software ES NOTE Norton Utilities and Norton Antivirus software must be deactivated before you install the software The installation might not run properly with the virus protection left running After installation you can restart your virus protection software 2 Close all software applications that might be running on your computer E3 NOTE Cameras should not be connected to the computer while installing the software ES NOTE Remove any copy protection devices attached to your computer during installation 3 Insertthe Carestream Molecular Imaging Software Version 5 X CD into your CD drive and double click the Carestream MI SE Installer mpkg icon to launch the installer application 4 The installer leads you through the installation process Make sure to select the Easy Install to install the software in the default directory and ensure full functionality of the system ES NOTE The Select Components window allows you to install MI without the database Only install the database in the location that you want to maintain images acquired from the system 5 While the software inst
58. ith your GL212 PRO v Follow the instructions in the Carestream Molecular Imaging Software Network Edition Administrator s Manual to install Carestream MI NE Software v Then proceed to Launching Carestream Molecular Imaging Software for the First Time later in this chapter If you have purchased the Carestream Molecular Imaging Software Regulatory Edition with Network Licensing Carestream MI RE to use with your Gel Logic 212 PRO Imaging System v Follow the instructions in the Carestream Molecular Imaging Software Regulatory Edition Network Administrator s Manual to install Carestream MI RE Software v Then proceed to proceed to Launching Molecular Imaging Software for the First Time later in this chapter Uninstalling a Previous Version MI or 1D Software Windows 1 Remove your MI or 1D copy protection device from your computer 2 Inactivate any virus protection software ES NOTE Norton Utilities and Norton Antivirus software must be deactivated before you uninstall the software The software might not uninstall properly with the virus protection left running After installation you can restart your virus protection software 3 Close all software applications that may be running on your computer ES NOTE Cameras should not be connected to the computer while uninstalling the software 3 3 3 p t ot o 8 4 Move any customized standards or templates and any projects from their
59. lb 12 Reinstall the cover plate with the retaining screws 13 Reinstall the module in the cabinet door see Installing the UV Epi illumination Module in Chapter 3 Installing Your System 14 Tighten the thumbscrews 15 Plug the power connector into the receptacle inside the GL212 PRO Imaging Cabinet above the door on the left side 16 Close the door making sure that the door is not pinching the power cord when it is closed If it is being pinched unplug the cable and rotate the connector 360 degrees to eliminate any twist that is in the cable mae NOTE The UV Epi illumination Module illuminates when UV Epi is selected in the GL212 PRO Acquire window 5 lt E m 5 3 ae 17 Unplug the power connector from the receptacle inside the cabinet above the door on the left side 18 Plug the power cord into the AC MAINS outlet 19 Turn on the cabinet power switch 5 9 5 10 Replacing the UV Transilluminator Platen Tools required v No 2 Phillips screwdriver Replacement parts O ON Oo v GL212 PRO UV Transilluminator Platen Kit Turn off the cabinet power switch Remove the power cord from the AC MAINS outlet WARNING The power cord is the primary disconnect device from the AC MAINS outlet Always turn the Illumination Selector off and disconnect the unit from the AC MAINS outlet before performing any service Open the GL212 PRO Imaging Cabinet door Pull the transilluminator
60. lumination after 5 minutes Click OK when you have finished setting your preferences 2 19 dp lt z 6 E O c E lt 6 Z 2 20 Setting Up Your System In this chapter you will learn how to set up your system so that you can begin taking pictures UY The GL212 PRO digital CCD camera provides mega pixel resolution that allows you to resolve closely spaced bands In addition the scientific grade sensor can integrate frames provides outstanding dynamic range and increase sensitivity 3 p t ot o v The GL212 PRO Acquire Software is integrated into Carestream MI and controls the camera during image capture and printing Once acquired images can be analyzed annotated and saved from within Carestream MI UY The GL212 PRO Imaging Cabinet may be equipped with both transillumination and epi illumination for both white and UV light The cabinet maintains a light tight environment and optimal focal length to image a wide variety of samples v The GL212 PRO automated zoom lens achieves proper focus and field of view for gels ranging from 4 25 x 6 0 cm to 24 5 x 33 cm v The 590 nm filter is specially designed to eliminate light other than 590 nm wavelength produced by ethidium bromide or other similar emission wavelength stained samples It permits maximum sample detection sensitivity by minimizing background v White Black Backdrop Screen positioned in the Gel Tray
61. ly examine it to determine that the glass filter is not broken A slight rattling sound is normal caused by the movement of small particles in the fluorescent bulbs 2 Open the cabinet door 3 With the filter glass facing you and the wire at the top of the module position the slotted arms over the thumbscrews 4 Tighten the thumbscrews 3 25 3 26 5 6 Plug the power connector into the receptacle inside the cabinet above the door on the left side Close the door making sure that the door is not pinching the power cord when it is closed If it is being pinched unplug the cable and rotate the connector 360 degrees to eliminate any twist that is in the cable pee NOTE The UV Epi illumination Module illuminates when UV Epi is selected in the GL212 PRO Acquire window If you do not have any other optional accessories proceed to Launching Carestream Molecular Imaging Software with your Carestream Gel Logic 212 PRO Imaging System later in this chapter Installing the UV Safety Shield The UV Safety Shield is designed to limit your exposure to UV when using the UV transilluminator for non imaging applications such as viewing gels or cutting bands ALWAYS WEAR PROTECTIVE CLOTHING EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER ALWAYS WEAR PROTECTIVE EYEWEAR FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURY TO EYE Never look directly into the lamp Exposure can cause eye and skin
62. mulation adds successive captures into a single accumulated image The maximum number of images accumulated is 32 generating up to a 14 bit image file The final accumulated image is automatically opened as an Carestream MI project These accumulated images expand the dynamic range of signal you can capture for more quantitative accuracy while also improving detection sensitivity v Time Lapse Exposure provides exposures at set intervals You can set the exposure time the time lapse interval the total exposure time and the total number of images Each file is automatically saved to your hard drive Type umm Time 0 100 Interval 10 000 Sec Stop After 10 000 Frame wm lt o 6 O lt lt 6 2 12 The Exposure Time defines the exposure The minimum and maximum exposure times are 0 001 seconds per frame and 16 seconds per frame respectively The minimal increment is 0 001 second The Coarse and Fine Exposure arrows allow you to adjust the exposure time in either coarse or fine increments This eliminates the need for you to use your keyboard The nterval is the time interval between the beginning of one exposure and the beginning of the next exposure The minimum and maximum intervals are 5 seconds and 24 hours respectively Stop After determines when to stop time lapse captures You can set stop criteria based on experimental duration or by the number of frame
63. n order to determine if a bulb is not functioning move the filter wheel to an open position and capture a short duration image with the UV Transilluminator turned on The resultant image shows bright bands of light at the location of each of the four 4 bulbs If a bulb is not working there will be a bright band missing ES NOTE The transilluminator consists of four 4 bulbs We recommend replacing all all four 4 bulbs when servicing the light source Tools required v No 2 Phillips screwdriver Replacement parts YW GL212 UV Transilluminator Bulb Kit 4 bulbs 1 Turn off the cabinet switch 2 Remove the power cord from the AC MAINS outlet WARNING The power cord is the primary disconnect device from the AC MAINS outlet Always turn the Illumination Selector off and disconnect the unit from the AC MAINS outlet before performing any service 3 Open the GL212 PRO Cabinet door 4 Pull the transilluminator drawer out far enough to allow access to the four 4 Phillips head screws on the sides of the Platen top 5 Remove the four 4 screws that attach the Platen top 6 Liftthe Platen from the UV Transilluminator and carefully set it aside 7 Remove all the bulbs from the housing by rotating 90 degrees in either direction This allows the pins to be lifted out of the slots at the top of the bulb holder jew Ol D 2 a 8 Insert a new bulb into the slots and rotate the bulb until a click is heard
64. ng these things and restarting your computer please call Carestream Molecular Imaging Technical Support at 1 877 747 4357 4 Choose the GL212 PRO Acquire window using the Select Your System dialog box 5 The GL212 Pro Acquire window appears Your installation is complete 6 Proceed to Installing Emission Filters 3 29 Installing Emission Filters The Automated Filter Wheel facilitates the use of up to six 6 filters A 590 nm filter is included in the package Additional filters including 440 nm 535 nm 570 nm and 670 nm are available from Carestream Molecular Imaging or your Carestream Molecular Imaging dealer See Appendix A Parts and Accessories To install the filters Carestream MI software must be running as the software controls the position of the Emission Filter Wheel WARNING To avoid damaging the automated Emission Filter Wheel do not move the wheel manually 1 Open the GL212 PRO Imaging Cabinet door 2 Fromthe GL212 PRO Acquire window select the Preferences button The Preferences window appears 3 Click the Emission Filter Change button The Edit Filters dialog box opens Edit Filters Filter Position A nm Label 4 Open Open Load 1 m j A 535 535 Load 3 v 50 a sso 9 TS so e706 4 Click the Load button of the filter position you want to install The Emission Filter Wheel moves to the correct loading position 3 30 5 Carefully position the filte
65. ngs can be conveniently applied to future image captures You can make modifications to the settings i e change exposure time or delete a setting using the Update Settings Update Settings or Delete Settings option respectively Delete Settings The Settings pop up menu also contains a number of factory defined settings useful for common laboratory imaging applications Coomassie Blue SYBR Green Colony Counting Microtiter Plate EtBr gel Swells Saved settings include v Type v Exposure time 2 o lt m D 3 o v Number of exposures v Illumination correction MdIAJOA V Image orientation V Preferences selections YW Saturation levels E3 NOTE The Settings option does not save the user name or annotations 2 9 2 10 pee NOTE Custom settings are saved as individual files in C Program Files Molecular Imaging MIApplicationGL 212 AGL 212 Settings Windows or in the Molecular Imaging MI Application MI Extensions GL 212 Settings Macintosh subfolders User Name User Name Use the User Name text edit box to type in your name To save your name for future experiments choose Add User from the pop up menu The name appears in the menu Names can be deleted in the same manner by choosing Delete User Annotations Annotation You can tag your image with an Annotation bar with up to 48 characters of text In addition the Annotation bar lists the time and date of the imag
66. nical Support at In US and Canada 877 747 4357 or world wide 203 786 5657 between the hours of 8 00 a m and 6 00 p m Eastern Standard Time Monday through Friday VY E mailing Carestream Molecular Imaging Technical Support at molecular support carestreamhealth com UV Faxing Carestream Molecular Imaging Technical Support at 203 786 5656 General Instructions for Cleaning and Decontamination For Return of the GL212 PRO All returned material must be cleaned and decontaminated prior to shipping To meet Federal and State Regulatory and Safety standards please follow the decontamination procedure given here if radioactive materials are used with this product or are used in the vicinity of where this apparatus has been used or stored General Cleaning Procedure For materials not contaminated with biological or radiological substances components may be gently washed using water or an ammonia based spray cleaner using soft lint free cloth or lens paper WARNING Do not expose Platen to strong chemical solvents such as ketones A and hexanes This may permanently damage the Platen and will void your warranty Radiological Decontamination Procedure WARNING We cannot accept return of products which are contaminated with A any radioactivity For beta emitting isotopes such as 32P use a GM type radioactivity meter calibrated in counts per minute CPM to determine the background readings for your work area Wearing latex glo
67. nm 302 nm or 254 nm optional accessories v White Light Transilluminator Module optional accessory Carestream Molecular Imaging Software 1 v Carestream Molecular Imaging Software CD 1 v Carestream Molecular Imaging Software User s Guide 2 WY Copy Protection Device 2 v Registration Card or v Carestream Molecular Imaging Software Network Edition Custom Package Carestream Molecular Imaging Software Network Edition CD 1 Carestream Molecular Imaging Software Network Edition Administrator s Manual 1 Carestream Molecular Imaging Software User s Guide 2 System Requirements These are minimum specifications however we cannot ensure that all hardware and software systems are compatible For optimal performance we strongly recommend dedicating a computer exclusively for use with your imaging system Minimum System Requirements Windows v Personal computer with a USB port and IEE 1394 FireWire port v Pentium IV or equivalent processor greater than 2 GHz is recommended v Windows XP Service Pack 3 or greater or Windows Vista Business Service Pack 1 or greater operating system software mh ES NOTE Check your operating system version by right clicking on the My Computer icon and then on Properties v 17 in display 1280 x 1024 resolution VY 2 GB recommended VY Minimum 10 GB of available hard disk space YW CD drive CD RW drive recommended v TCP IP v Internet Explorer 7
68. o shown T Attach the Transilluminator Cable Connector larger connector into the keyed connection on the left side of the ceiling in the imaging cabinet 3 e o E3 Ke c E 8 Attach the two 2 Epi White Light Cable Connectors smaller connector into the keyed connections on both sides of the ceiling in the cabinet 9 Connect the Camera Computer FireWire Interface Cable to the back of your GL212 PRO Imaging Cabinet 3 21 10 Attach the IEEE 1394 FireWire plug into your computers IEEE 1394 FireWire port WARNING Your computer should be turned off when connecting your A camera to your computer 11 Connect the GL PRO Serial Communication Cable to the Serial Port to the back of your GL212 PRO Imaging Cabinet 12 Attach the GL Serial Communication Cable to the Serial Port on your computer 13 Insert the Line Power cord in the IEC power input and connect into the AC MAINS source 14 Tum the power switch located on the back of unit to the ON position 15 The hardware installation of the base system is complete v If you do not have any optional accessories proceed to Launching Carestream Molecular Imaging Software with your Carestream Gel Logic 212 PRO Imaging System later in this chapter v If you have purchased accessories proceed to Carestream Gel Logic 212 PRO Accessories Installation 3 22 Carestream Gel Logic 212 PRO Accessories Installation
69. oducible and may be corrected by dividing the sample image by an illumination reference image It is important to capture a reference image of the illumination field using the same camera settings f stop zoom used at the time you are capturing the image to be corrected Prior to capturing the image select the Illumination Reference checkbox 2 Capture an image as described in Capturing Images earlier in this chapter E3 NOTE Do not adjust the f stop zoom exposure time or placement of the Epi Illumination pads Upon completion of the capture the illumination correction dialog box appears Remove the sample from the imaging cabinet and clean the surface Enter a name in the Output Name text edit box ao a Oo Click Continue The illumination reference is applied and the output file opens as a new Carestream MI project Ex NOTE You can not do an illumination reference correction if you have saturated pixels in your images Reduce the exposure time of your image to eliminate the risk of saturating the illumination reference image Adding an Annotation Bar to the Printed Image You can tag your image with an Annotation bar which contains the time and date of the capture of the image the exposure setting sample type and up to 48 characters of text The text will appear on the long axis at the top of the image in 12 point Helvetica Bold as white text on a black background ES NOTE All items in the Annotation ba
70. of Molecular Probes Incorporated All other products or name brands are trademarks of their respective holders Should you have any questions concerning this product contact Technical Support Carestream Mo lecular Imaging Carestream Health Incorporated 4 Science Park West New Haven CT 06511 USA For laboratory research use only Not suitable for human or animal diagnostic or therapeutic use Table of Contents IntFOGUCLODI 4a kh caca CE RR ea C CR RC ek QE RC CR RC RR CR Ben 1 1 About the Users Guides sis 44 05 m E REX gees gute Rr e EEQUPOE Sd ee 1 2 CONVENTIONS 2d e Gre el en ween eed e edt o e E bebe eae e 1 2 Navigating Through the User s Guide 0 00 00 eee eee eee 1 3 Package Contents sete cena P ete NARA RRO E Bae Sars ARE SH 1 4 System Requirements 0 20 cece cece eee tener hh he rrr 1 5 Minimum System Requirements Windows 0 000 c cece eese 1 5 Minimum System Requirements Macintosh l l 1 5 Technical Support 22e EE RIDE RR CE P NR Eee 1 6 Carestream Molecular Imaging Technical Support 0 0 000002000 1 6 Registering Your Systems sigs euren EFIE a Rond 1 7 System OVBIVIBW JJ aa vacantem e ROCCO ROCA Rom sere s 2 1 The Carestream GL212 PRO Imaging Cabinet esses 2 2 The Carestream GL212 PRO Acquire Software llllsleslelesesn 2 6 The GE212 PRO Image Preview 12k ova de nage ves ERR ERR ER M 2 8 Capture Section of the GL212 PRO Acquire Window
71. pe the camera lens with chemically treated eyeglass lens tissue because it may scratch the lens 9 Reinstall the filter See Installing Emission Filters in Chapter 3 Setting up Your System Cleaning the White Black Epi illumination Pads The White Black Epi illumination Pads are easy to clean VY For material not contaminated with biological or radiological substances the pads may be washed with a non abrasive detergent and rinsed with deionized water VY For materials contaminated with biological substances prepare a 5 bleach solution in water Wipe down with the bleach solution using a clean cloth or sponge Rinse thoroughly with deionized water 5 lt E 5 B 3 e v For materials contaminated with radiological substances decontaminate with a radioactive decontamination detergent following manufacturer s instructions and your institutional safety protocols for decontaminating and handling radioactive waste v To dry use a soft cloth paper towel or allow to air dry 5 3 5 4 Repairing Your Carestream Gel Logic 212 PRO Imaging System Periodically some parts of the GL212 PRO may need to be replaced This section details the user serviceable repairs WARNING The power cord is the primary disconnect device from the AC MAINS outlet Always turn the switch the cabinet off and disconnect the unit from the AC MAINS outlet before performing any service Replacing the UV Transilluminator Bulbs I
72. port using a special Serial Communi cation Cable 6 lt o 6 O c E lt 6 2 5 2 6 The GL212 PRO Acquire Software The GL212 PRO Acquire window supports a wide variety of image capture methods including single capture exposure multiple capture exposure accumulated into a single file or as separate files and time lapse exposures Using these exposure modes the minimum and maximum exposure times are 0 001 second and 16 seconds respectively The GL212 PRO Acquire window is accessed from within Carestream MI Choose Select Digital Camera from the File menu and GL212 PRO from the Digital Camera pop up menu The GL212 PRO Acquire window appears Let s review Gel Logic 212 PRO Capture Settings Current Session Type Singe Time 0 003 Sec No Exposures fi Y Expt Duration 0 003 sec A The Image Preview window displays continuous successive live captures B The Capture section contains options to apply predefined settings record your name and add annotations on the image C The Exposure section allows you to select the various exposure options neces sary to capture images including the auto exposure mode You can adjust exposures using the course and find adjustment buttons D The Buttons section contains the Stop Capture Preferences Quick Print and Done buttons and the Progress bar and Elapsed Time text boxes used to navi gate through an
73. provides good non reflective surfaces for capturing epi illuminated images v The Gel Tray supports the White Black Backdrop Screen and also provides a gel cutting surface for excising bands from your gel when using the optional UV Safety Shield v The White Light Transillumination Module optional accessory provides white light transillumination that is ideal for imaging translucent samples e g Coomassie stained gels or plate assays v The UV Epi illumination Light Modules are optional accessory The light accessory is easily installed inside the GL212 PRO Imaging Cabinet door Wavelengths available include 370 nm 306 nm or 254 nm v The UV Safety Shield optional accessory is designed to protect you from UV when using the UV transilluminator for non imaging applications such as viewing gels or cutting bands 3 1 3 2 Environmental Requirements The GL212 PRO designed to operate effectively within the temperature and humidity ranges typically found in laboratories For effective operation the temperature and relative humidity should be v Temperature 18 to 28 C v Relative Humidity 7596 non condensing v Altitude This product is designed for use at altitudes up to 2000 meters This product is designed for indoor use only This product meets Pollution Degree 2 standards in accordance with IEC 664 Electrical Requirements There are several versions of the GL212 PRO available v 120VAC 60Hz 2A
74. r thread side down into the Filter Tool while pressing firmly on the Filter Wheel Tool handle Be careful not to touch the filter element Gently release your grip as you position the filter in the filter wheel The filter will drop into position 6 Click OK T Enter the wavelength and a label for the filter you have loaded in the Wavelength and Label text edit box 3 CD F Ke o 8 Repeat Steps 4 7 for all the filters that you want to install 9 Click OK The table below defines the appropriate filter for many common stains and dyes Dye Stain Filter nm Coomassie Blue 590 or none Ethidium Bromide 590 Fluorescein 535 Oregon Green Gel Star 535 SYPRO Orange SYBR Green 535 SYBR Gold 535 Proceed to the next section Focusing the Camera 3 31 3 32 Focusing the Camera Place a target directly on the Platen A ruler or business card is a good target to use Select White Light Epi from the Illumination Source pop up menu Select the Emission Filter position to Open Do not select an emission filter 1 2 3 4 Setto view entire test target 5 Click Preview The GL212 PRO Preview window appears 6 Click Focus checkbox A selection rectangle appears Gel Logic 212 PRO r musususmsuma Lens FOY fo 90 Ge go T Illumination Current Session White Light Epi kod gt Apply Correction Emission Filt
75. r are separate objects once submitted to Carestream MI and displayed in the Annotations window You can change the font style color or size prior to printing however the items are grouped You must ungroup the items prior to editing 1 Type text up to 48 character in the Add Annotation text edit box Annotation 2 The Annotations bar is appended to the image upon printing E3 NOTE Each time you take a picture the Add Annotation text edit box are cleared ES NOTE You can display or hide the Annotation bar by selecting the Show Hide Annotation bar from the Annotations menu i9 S xe et 5 Ke 3 D Q D a 4 13 Using the Transilluminator for Non Imaging Applications The UV Transilluminator provides broadband UV ideal for visualizing or cutting out bands from fluorescent samples like ethidium bromide stained gels 1 Place the sample on the UV transilluminator Platen VY For UV transillumination place the sample on the Platen 2 Insert the UV Safety Shield into the Safety Shield slots in the front of the UV transilluminator 3 Press the UV transilluminator button located on the front of the transilluminator ALWAYS WEAR PROTECTIVE CLOTHING EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER ALWAYS WEAR PROTECTIVE EYEWEAR FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURY TO EYE Never look directly into the lamp Exposure can cause eye and skin allergy and allergic rea
76. re spread over a 4 25 x 6 cm area At the largest field of view setting 8 mm the pixels are spread over a 24 5 x 33 cm area 46 microns pixel An important consideration is the number of pixels that are applied to the smallest feature of interest 5 If the preview is out of focus click on the Focus checkbox use the Focus buttons to adjust the focus 00 ES NOTE If you have problems focusing a software assisted focusing tool can be used See Using the Focusing Tool later in this chapter i9 S pe r c ef 5 Ke 3 D on 6 Choose your Exposure Type v Single Exposure takes one 12 bit image After capture the image file automatically opens as a Carestream MI project 4 7 v Multiple Exposure Final Accumulation adds successive captures into a single accumulated image The maximum number of images accumulated is 32 generating up to a 14 bit image file The final accumulated image is automatically opened as a Carestream MI project These accumulated images expand the dynamic range of signal you can capture for more quantitative accuracy while also improving detection sensitivity v Time Lapse Exposure provides exposures at set intervals You can set the exposure time the time lapse interval the total exposure time and the total number of images Each file is automatically saved to your hard drive ve a Time 0 100 Sec Interval 10 000 Sec Stop After 10 000 Frame
77. respective subfolders in your existing MI or1D X X folder to a temporary folder outside the MI or 1D folder If you are currently using the MI database also move the database folder Uninstall previous version s of MI or 1D Software v Uninstall MI by choosing Control Panel from the Start menu and Add Remove Programs Windows XP or Uninstall a Program from the Program menu Windows Vista Scroll to locate MI X X Click to select Click the Add Remove button Windows XP or Uninstall Change Windows Vista and follow the on screen instructions to uninstall You can also uninstall the application using your MI CD The installer shield automatically detects that you have the software loaded and offers an uninstall option A window informs you as each software has successfully been removed v Uninstall ID by choosing Programs from the Start menu and selecting Remove 1D X X from the 1D X X submenu v Uninstall MI NE or ID NE following the instructions provided in your Network Administrator s Manual Uninstall additional software Windows XP by choosing Control Panel from the Start menu and Add Remove Programs or Uninstall a Program from the Program menu Windows Vista Scroll to locate Sentinel System Driver Click to select Click the Add Remove button Windows XP or Uninstall Change Windows Vista and follow the on screen instructions to uninstall A window informs you as each software has successfully been removed I NOTE Automate
78. retaining clips on the right side of the cabinet to retain the White Light Transilluminator Connector Cord E3 NOTE The White Light Epi illumination module illuminates when White Light Epi is selected in the GL212 PRO Acquire window 6 If you do not have any other optional accessories proceed to Launching Carestream Molecular Imaging Software with your Carestream Gel Logic 212 PRO Imaging System later in this chapter 3 24 Installing the UV Epi illumination Light Module Optional Accessory The UV Epi illumination Light Accessory is an optional accessory The light accessory is easily installed inside the GL212 PRO Imaging Cabinet door ALWAYS WEAR PROTECTIVE CLOTHING EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER ALWAYS WEAR PROTECTIVE EYEWEAR FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURY TO EYE Never look directly into the lamp Exposure can cause eye and skin allergy and allergic reactions Medications or cosmetics may increase your sensitivity to ultraviolet radiation Consult physician before operating this product if you are using medications or have a history of skin problems or believe yourself especially sensitive to sunlight For Laboratory Use Only DANGER Ultraviolet radiation emitted from this product Avoid exposure A dn umas OO WARNING The UV source does not operate when the door is open Do not attempt to override this feature 1 Unpack the module and careful
79. romes within the experimental sample The Filter pop up menu selects the emission filter used during capture The system includes a 590 nm band pass filter The 590 nm filter is specially designed to eliminate light other than 590 nm wavelength produced by ethidium bromide or other similar emission wavelength stained samples It permits maximum sample detection sensitivity by minimizing background Additional filters including 440 nm 535 nm 570 nm and 670 nm are available from Carestream Molecular Imaging or your Carestream Molecular Imaging dealer See Appendix A Parts and Accessories 2 15 dp lt z 6 E O c E lt 6 The GL212 PRO Preferences Dialog Box You can choose a variety of options using the GL212 PRO Preferences dialog box Let s review the dialog box options GL212PRO Preferences Image Capture Submit Rotated R Options O EEVEE Camera Delay None j Emission Filters Change White Light amp c5 Trans Intensity aan Options Print Immediately Print Image Actual Size iv Show image saturation jw Turn off illumination source after 5 minutes of inactivity Deun M Image Capture Preferences The Image Capture Preferences allows you to select v Submit Rotated provides the option of automatically rotating a newly captured image to a desired orientation as they are downloaded from the camera Rotate PR e ul The image is in the de
80. s Intensity The White Light Transillumination Intensity allows you to set the intensity of light used in the illuminator Print Preferences The Quick Print feature is used when a printed copy is all that you require When you click Quick Print an image is captured and sent directly to your printer The Printing Preferences allows you to control the various print options from within the GL212 PRO window The Options pop up menu allows you to select Print Immediately Print Immediately bypasses the standard Print dialog box and immediately prints the image on the current default printer Once printed the file is deleted Open Print Dialog opens the Print dialog box where you can select print parameters Once printed the file is deleted The Print Image pop up menu sets the image size By 3x 5 inches Alternatively chose an image size of 3 x 5 in 4 x 6 in 5 x 7 in or Full Page 4 x 6 inches 5x 7 inches Ful P Show Saturation Checkbox JV Show Saturatio Click the Show Saturation checkbox to turn image pixels red when the pixel is saturated Saturated pixels adversely affect the quality of your image analysis since the signal readings do not accurately measure intensity Use this option to aid you in selecting the ideal exposure conditions aperture and exposure time Warning Preference You can set a reminder to display on screen to remind you to turn off your illumination M Reminder to turn off il
81. s The maximum duration time is 200 hours The maximum number of image frames that may be captured is 100 The Exposure Time Time 0 003 Sec The Exposure Time text edit box defines the exposure The minimum and maximum exposure times are 0 001 second per exposure and 16 seconds per exposure respectively The time units can be set as seconds or minutes The minimum increment is 0 001 second for short exposures Use the Auto Exposure button for automated exposure determination c 90 Use the Coarse and Fine Exposure arrows to adjust the exposure Fine o time in either coarse or fine increments This eliminates the need for you to use your keyboard No Exposures E When you have selected Multiple Exposure Type you can accumulate up to 32 captures into a single image This is especially useful when you have faint signal You select the number of exposures by typing in a value or by selecting one of the predefined options from the pop up menu The Expt Duration indicates the total capture duration in hours The software will automatically calculate the total duration of the exposure based on the number of exposures and the time of each exposure Buttons on the GL212 PRO Acquire Window The buttons on the GL212 PRO Acquire window execute functions Let s review The Preview Stop button initiates the Preview mode When the Preview is running the button toggles to the Stop button The Capture button initiat
82. s Windows or select About This Mac under the Apple menu item Macintosh v The type of image you are capturing or analyzing v The problem you are having and what you were doing when the problem occurred Please note the exact wording of any error messages including any error numbers displayed Carestream Molecular Imaging Technical Support Contact Carestream Molecular Imaging Technical Support by v Utilizing our World Wide Web support pages at mi carestreamhealth com v Calling Carestream Molecular Imaging Technical Support at In US and Canada 877 747 4357 or world wide 203 786 5657 between the hours of 8 00 a m and 6 00 p m Eastern Standard Time Monday through Friday v E mailing Carestream Molecular Imaging Technical Support at molecular support carestreamhealth com v Faxing Carestream Molecular Imaging Technical Support at 203 786 5656 1 6 Registering your System It is important for you to register your Carestream Molecular Imaging System Once registered you will receive information on maintenance releases upgrades and exciting new products Register by filling out and returning the registration card included with your software package You can also register any time on line If your computer connects to the Internet select Register MI from the Help menu Windows or Register Online from the Apple menu item Macintosh 1 7 ucl 5 x 9 Q 2 ct o 1 8
83. s 1 4 installation 3 1 3 33 maintaining 5 1 5 8 X pu overview 1 1 2 1 2 19 registering 1 7 regulatory information W 6 repacking W 5 repairing 5 4 5 8 requirements 1 5 T Technical Support 1 6 6 2 W 1 W 3 Time lapse exposure 2 11 2 12 4 8 troubleshooting 6 1 6 8 U uninstalling software3 3 3 4 3 9 Updating settings 2 9 2 10 2 16 2 19 4 10 User name option 2 9 2 10 2 16 2 19 4 10 User s Guide about the 1 2 Carestream MI 1 2 conventions 1 2 navigating 1 3 User section 2 9 2 10 2 16 2 19 4 10 UV Transilluminator 3 20 3 21 UV Epi Illumination Light 3 25 3 26 UV Safety Shield 3 27 use of 4 14 W warranty W1 W 5 coverage W 1 decontamination W 4 W 5 limitations W 2 registration 1 7 regulatory W 6 service W 3 time period W 1 white light epi illumination 2 4 2 5 4 6 white light transillumination 2 4 2 5 4 6 Windows requirements 1 5 software installation 3 3 3 7
84. s secure pee NOTE Windows XP Users A Welcome to the Found New Found Hardware Wizard dialog box appears on screen You will be asked if you want the Windows update to search for software Click No not at this time and advance through the installation wizard ES NOTE Windows Vista Users Installing Device Driver Software dialog box will appear in the lower right hand corner Upon completing installation a message appears informing you that Rainbow USB Superpro Device Driver Software installed successfully E3 NOTE If you are upgrading from a previous version of the MI or 1D Software and received a new copy protection device you must attach both the old and new copy protection devices to your computer After you launch the newest version for the first time your old copy protection device is be deactivated Remove and discard the old key pee NOTE If your computer has multiple USB ports you can plug the copy protection device into any of them The software when launched checks all available USB ports 2 Proceed to Windows Power Settings later in this chapter Windows Power Settings The GL212 PRO requires that your computer Energy Saver features be disabled This process by may differ depending upon the Windows software installed Windows XP 1 Click the Power Options icon from the Start menu and the Control Panel 2 Click Power Schemes tab and select Always On from the Power Schemes pop up menu 3 The settings
85. sh Power Settings Macintosh Power Settings The GL212 PRO requires that the Energy Saver settings on your computer be disabled To disable the system sleep features 1 Choose Energy Saver from the File menu and the System Preferences submenu or from System Preferences on the Dock The Energy Saver window opens Energy Saver Sleep Schedule Options L Put the computer to sleep when it is inactive for I 1 min 15 min lhr 3hrs Never V Put the display to sleep when the computer is inactive for 1 min 15 min lhr 3hrs Never Never letting your display sleep may shorten its life _ Put the hard disk s to sleep when possible Ja JF Click the lock to prevent further changes 2 Click the Sleep tab and set the Computer and Display sleep sliders to Never Ensure the Put the hard disk s to sleep when possible checkbox is unchecked 3 Proceed to Launching Carestream Molecular Imaging Software for the First Time 3 12 Launching Carestream Molecular Imaging Software for the First Time 1 Launch Carestream MI software by clicking on the MI icon found on the desktop 2 The Carestream MI Security Setup dialog box appears You must select either No Login or Login Required from the pop up menu Click Continue Carestream MI Security Setting B Please choose a security level for this software Security Level Noton e Ex NOTE The selected Carestream MI Security feature applies to
86. ted cabinet integrates both transillumination and epi illumination for both UV and white light excitation allowing you to image a wide variety of fluorescent and colorimetric samples The broadband UV excitation peak at 306 nm provides for the detection of a broad range of fluorescent dyes including ethidium bromide SYBR Green and SYBR Red The white light illumination is ideal for illumination of protein gels blots and plates The GL212 PRO Acquire Software module running within Carestream MI allows you to v Image electrophoresis gels microtiter plates and colony and plaque assays v Analyze your image for molecular weight mass optical density and intensity measurements v Annotate and prepare your images for publication v Generate hard copy prints at a fraction of the cost of instant photography v Easily share or transmit image files over local or worldwide networks Carestream Molecular Imaging is dedicated to providing cutting edge imaging product systems for scientific research applications For more information on screen products for scientific imaging applications please visit us on the worldwide web at mi carestreamhealth com 1 1 d S e Q O eX e 2 1 2 About the User s Guide The Carestream Gel Logic 212 PRO User s Guide provides you with all of the information you need to capture images It is designed to be used in conjunction with the Carestream Molecular Imaging Soft
87. th com w Calling Carestream Molecular Imaging Sales at In US and Canada 877 747 4357 or world wide 203 786 5657 between the hours of 8 00 a m and 6 00 p m Eastern Standard Time Monday through Friday For up to date dealer information visit our WEB site at mi carestreamhealth com Catalog Number Description Gel Logic 212 PRO Emission Filters 8103004 440 nm Filter 52 mm 8456998 535 nm Filter 52 mm 8420325 570 nm Filter 52 mm 1192871 590 nm Filter 52 mm 8519027 670 nm Filter 52 mm Accessories 8056244 Gel Logic PRO 306 nm UV Epi Illumination Module 110VAC 1672450 Gel Logic PRO 306 nm UV Epi Illumination Module 220 240VAC 8257222 Gel Logic PRO 254 nm UV Epi Illumination Module 110VAC 8034662 Gel Logic PRO 254 nm UV Epi Illumination Module 220 240VAC 8105777 Gel Logic PRO 370 nm UV Epi Illumination Module 110 VAC 1282466 Gel Logic PRO 370 nm UV Epi Illumination Module 220 240VAC 8737678 Gel Logic 212 Pro White Light Table U o T Qo gt Q O D fe A 1 A 2 Catalog Number Description Accessories continued 1661669 Gel Logic 212 Pro UV Shield 8406761 Gel Logic Gel Tray Accessory 1941442 Gel Logic Fluorescent Ruler 1411701 Gel Logic Field Flattening Screen 1693803 Mobile WorkStation 4 foot Computer amp Printing Accessories 1903426 Desktop PC w 17 Flat Panel Monitor
88. tic wrapping Repack the GL212 PRO components in their original boxes If you have discarded the original packing material call Carestream Molecular Imaging Technical Support to arrange for delivery of new packing material Regulatory Information This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Any changes or modifications not expressed by the party responsible for compliance could void the user s authority to operate the equipment Where shielded interface cables have been provided with the product or specified additional components or accessories elsewhere defined to be used with the installation of the product they must be used in order to ensure compliance with FCC regulation Disposal of these materials may be regulated due to environmental considerations For disposal or recycling information please contact your local authorities
89. u and the Control Panel Click Power Schemes tab and select Always On from the Power Schemes pop up menu The settings for Turn off monitor Turn off hard disks System standby and System hibernates functions become Never The Gel Logic camera appears as an Unknown Device in Windows Device Manager The Gel Logic driver must be replaced Right click My Computer Properties Hardware tab Select the Device Manager Find and expand imaging devices or camera Right click on Gel Logic Imaging System and click Uninstall Close windows and restart machine On restart Found New Hardware Wizard appears Select No not this time Select Install from a list or specific location Select Don t search I will choose driver to install Select Gel Logic system Click Finish when wizard finishes copying files oO em iex 0 n F 9 e iml gt ie Problem Probable Cause Solution Cannot capture images using Macintosh system Older version of Quicktime software not compatible Update the Quicktime software to the most recent version Error communicating with Serial connection problem Make sure that the serial cable is GL212 PRO camera using connected through the serial to Macintosh OS X USB cable accessory Error 20005 The copy protection device is not Connect the copy protection found device and restart your computer Error 12001 The
90. uently passwords expire When a user password expires you will be prompted to enter a new password the minimum is 2 days v SMTP Server This mail server can be used for sending out e mails and broadcasting messages This is a mandatory field that must be entered The SMTP mail server is required when users click the Forgot Password button v If the checkbox Enable the forget password button for administrator is checked A user with system administrator privileges can use the forget password function An additional feature of Carestream MI Image Database is the ability to broadcast e mail messages to users Administrators can enter a subject and message in the fields provided and click Send All users receive the broadcast message To send receive a message the user must have a valid e mail address entered Carestream Image Database Molecular Imaging Software V eicome investigations Search Users JobFunctons MyAccount BroadcastMessaoe GenerGetup Logout yourname yourcompany com 30 must be more than 2 days Outgoing Email Server mail yourcompaey com Enable the Forgot Password button for system adrrenisirators 8 Click Logout to exit Carestream MI Image Database On the next restart of Carestream MI new users must enter their username and password The user will then be prompted to access the web portal to change their password Carestream o t 2 Molecular Imaging Software s re 9 Shut down Car
91. v 230VAC 50Hz 2A Supply voltage fluctuations should not to exceed 10 This product is designed to withstand transient over voltage according to Installation Category II Refer to your computer operators s manual for its electrical requirements Space Requirements The GL212 PRO overall weight and dimensions are as follows VW 54 6 cm 21 5 inches length and 42 cm 16 5 deep and 62 6 cm 24 6 inches high v 57 1 kg 125 6 pounds We recommend that the instrument and computer be placed on a bench or table that is level and capable of supporting 68 kg 150 pounds located not more than 3 feet 91 cm from an electrical outlet The GL212 PRO including computer CPU monitor and keyboard requires a minimum space 48 inches wide 20 inches deep and 35 inches high 122 cm x 51 cm x 69 cm These dimensions do not allow for a printer or other peripheral device Additional space adjacent to above or behind the GL212 PRO will allow for easier operation Installing the Carestream Molecular Imaging Software Windows Any prior versions of MI 1D and or camera software that are loaded on the computer must be uninstalled prior to installation If you are a new user proceed to Carestream Molecular Imaging Software Installation Windows Windows Vista and Windows XP installations may require administrator privileges If you have purchased the Carestream Molecular Imaging Software Network Edition Carestream MI NE to use w
92. ves survey the unit to be returned with the GM meter If any part of the unit is found to show readings higher than background wash the area using Count Off PerkinElmer Life Sciences or another similar commercially available detergent and paper towels If none are available a Formula 409 like solution or a mild detergent will do As you clean discard liquid and solid waste gloves and paper towels according to your local and institutional regulations for radioactive material disposal Continue washing until the GM meter reading for the contaminated area s is equal to or below background To decontaminate units where a GM meter is not useful for detection such as with 3H or 355 it will be necessary to perform swipes of the unit and detect using a scintillation counter The unit should be dry Wipe surfaces with dry paper circles these are commercially available or you can make your own Areas can be charted so that individual swipes can be done on different surfaces to better isolate areas of contamination These swipes are then placed into individual scintillation vials with an appropriate fluor and then analyzed on a properly programmed scintillation counter If contamination above 200 disintegrations per minute dpm 200 cm dpm CPM efficiency is found wash the area as described above for beta emitting isotope decontamination After cleaning the area swipe it a second time to determine the amount of contamination remainin
93. vided by the computer manufacturer Days and Hours of Coverage Arrangements for service through Carestream Molecular Imaging Technical Support can be made Monday through Friday 8 00 a m to 6 00 p m EST in the United States except for locally observed holidays Hours of coverage outside the United States may vary Contact your local Carestream Molecular Imaging dealer for hours of coverage Warranty Repair Coverage 3 o gt m lt If the equipment does not function properly during the warranty period due to defects in either materials or workmanship Carestream Health Inc will at its option either repair or replace the equipment without charge subject to the conditions and limitations stated herein Such repair service will include all labor as well as any necessary adjustments and or replacement parts If replacement parts are used in making repairs these parts may be remanufactured or may contain remanufactured materials If it is necessary to replace the entire system it may be replaced with a remanufactured system Carestream Health Inc will also provide telephone assistance during the warranty period Limitations WARRANTY SERVICE WILL NOT BE PROVIDED WITHOUT DATED PROOF OF PURCHASE PLEASE RETURN THE WARRANTY REGISTRATION CARD WITHIN 30 DAYS OF PURCHASE THIS WARRANTY BECOMES NULL AND VOID IF YOU FAIL TO PACK YOUR INSTRUMENT IN A MANNER CONSISTENT WITH THE ORIGINAL PRODUCT PACKAGING AND DAMAGE OCCUR
94. ware User s Guide If you are like most users you will want to get started right away but before you do you need to have basic computer skills including v Launching applications v Using a mouse v Using pop up menus v Selecting and editing text v Saving and printing your work v Dragging and dropping objects Refer to your computer manual to become familiar with the skills listed above Conventions This User s Guide utilizes the following conventions v Menus and dialog boxes are displayed using Windows XP and may appear differently on your screen Any significant differences in commands between Macintosh and Windows platforms are noted in the text UW Menu commands tool names and window names are shown capitalized v Warnings tips and notes appear in the text like this ES NOTE Carestream Molecular Imaging provides maximum performance products V Important safety warnings appear in the text as follows WARNING This symbol is used in the User s Guide to designate a warning or caution statement WARNING This symbol is used in the User s Guide to designate where electrical shock is possible WARNING This symbol is used in the User s Guide to designate when there is a potential exposure to hazardous ultraviolet light WARNING This symbol is used on the instrument indicates protective earth D e D D Navigating Through the User s Guide The Carestream Gel Logic 212 PRO Imaging System Us
95. wing of the cabinet interior during UV illumination DANGER Ultraviolet radiation emitted from this product Avoid exposure ALWAYS WEAR PROTECTIVE CLOTHING EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER ALWAYS WEAR PROTECTIVE EYEWEAR FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURY TO EYE Never look directly into the lamp Exposure can cause eye and skin allergy and allergic reactions Medications or cosmetics may increase your sensitivity to ultraviolet radiation Consult physician before operating this product if you are using medications or have a history of skin problems or believe yourself especially sensitive to sunlight For Laboratory Use Only The Safety Shield must be in place when the door is open and the UV Transilluminator is activated UV will not be emitted at the Platen surface if the UV Safety Shield is not in place Replace the UV Safety Shield if cracked or damaged Personal Protective Wear If your hands are in close contact with the Platen surface or anywhere behind the UV Safety Shield to prevent injury it is imperative that appropriate gloves be worn i e nitrile Latex gloves are also effective however latex may present an allergic reaction in some individuals In addition long sleeved lab coat or other tightly woven material will be effective in reducing UV exposures Protective eye wear should be worn by users when the Platen surface is pulled out WARNING The Line Cord is the pr
96. your capture conditions including sample placement aperture field of view FOV and exposure time You may want to use the white light epi illumination setting or ambient light to aid in positioning your sample E3 NOTE The Show Saturation feature Preferences displays image pixels red when the pixel is saturated Saturated pixels adversely affect the quality of your image analysis since the signal readings do not accurately measure intensity Use this option to aid you in selecting the ideal exposure conditions aperture and exposure time 4 5 4 6 2 Select your illumination type from the Type pop up menu the exposure time defaults to a typical exposure time White Light Trans Y white Light Trans v White Light Trans using the White Light Transilluminator Module an optional accessory is ideal illumination for non opaque samples plates and visible dye stained gels Y UV Trans is ideal illumination for providing illumination of fluorescently stained images i e ethidium bromide or SYBR Green v White Light Epi is ideal illumination for imaging samples such as plates and blots v UV Epi is ideal illumination for imaging samples like TLC plates using the optional accessory UV Epi illumination Module s Wavelengths available include 370 nm 306 nm and 254 nm Adjust the Exposure Time and or Aperture to display the desired features without saturating pixels displayed in red on screen the pixels in
97. your new Carestream MI installation for all users v In No Login Mode all users gain access to Carestream MI bypassing the login screen v In Password Mode tequires you to enter your User Name and Password every time Carestream MI starts up ES NOTE The initial installation of Carestream MI comes with a single user with the User Name Admin and the Password password Use this User name to gain access to Carestream MI Image Database to set up users 3 13 3 CD 4 c Ke 3 The Login window appears Enter Admin as the Username and password as the Password The Host Name default value will cause Carestream MI to search your local drive for the Carestream MI database If the Carestream MI database is located on another machine or file server enter the IP address of that machine in the Host Name field Click Login Username Password B Cancel Login Host Name 127 0 0 1 Port 8080 Context E3 NOTE The initial installation of Carestream MI comes with a single user with the User Name Admin and the Password password Use this User name to gain access to Carestream MI Image Database to set up users E3 NOTE If no Carestream MI database is detected at the Host Name location you will be asked if you would like to continue without connection to a database 4 If you selected No Login in the Carestream MI Security Setup dialog box future launches of Carestream MI will skip t

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