Perfusion Valve Controller User`s Manual
Contents
1. Perfusion Valve Controller Model VC 6 WARNER INSTRUMENTS A Harvard Apparatus Company Publication 5710 001 REV B WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes2. an adjusted by raising or lowering the reservoir holder as well as by adjusting the height of each reservoir within the holder The table below lists the approximate flow rates for a reservoir at the specified height with the supplied Tygon tubing Reservoir height Approximate flow rate 61 cm 24 in 14 ml min aema em n Rear Panel e The Event Marker output produces a 500 ms logic level output 5 V each time a valve is turned on Atall other times the Event Marker output is low 0 V e A 15 pin D type female connector is used to connect the VALVE BRACKET and valves to the CONTROLLER A polarized 3 conductor IEC320 CEE 22 connector is used for line mains power input to the instrument A removable cordset terminated with a NEMA 5 15P connector is standard An alternate cordset may be supplied when local circumstances dictate different mains voltages and connections Contact our offices for details A fuse holder contains a protective fuse in series with the high side brown or black wire of the mains The holder accepts 1 4 x 1 1 4 inch 6 35 x 31 8 mm fuses of the type indicated Publication 5710 001 REV B Perfusion Valve Controller Model VC 6 Warner Instruments Maintenance Cleaning Do not use alcohol aromatic hydrocarbons or chlorinated solvents for cleaning They may adversely react with the plastic materials used to man ufacture the system If salt solution spills on the valve as
3. as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 2 Perfusion Valve Controller Model VC 6 Warner Instruments Table of Contents INTOQUCION ELA as 2 Operating DIrectionS serescereseeresee recensisce ne seenenee 3 OUI CENERI RE aaasta E A A ORTA 3 AO COEN id 5 Instructions for Use naaa ra 6 Flow AdjUSIMONE oia 6 Fear Panel iia ia 6 Maintenant enisinia iiia aiaia 7 A er e ES AA a 7 Watlanyi catiganaian iii 8 Sp cifications 1 8 Publication 5710 001 REV B Perfusion Valve Controller Model VC 6 Warner Instruments Introduction The VC 6 Valve Control System lies at the heart of a multi valve perfusion sys tem designed to automate and control the delivery of solutions to Warner Instruments imaging and recording chambers However its flexible design allows the system to be used in many applications not using Warner equip ment The basic system includes the valve controller a valve bracket with valves con necting cable C Flex tubing and an MP Series manifold The complete system includes all of the above plus a ring stand six 60 cc syringes 20 feet of Tygon tubing and an assortment of tubing connectors The controller can independently regulate the function of up to six valves The system can be purchased in a two or four valve configuration and can be easi ly expanded by simply adding more valves Individual valves can be cont
4. ctors 1 16 ID tube to tube Y connectors 1 16 ID Luer to female barb connectors and stopcock with Luer connector Connectors are shown in order below Plastic tubing can be attached to these connectors with little effort Basic Systems are supplied with a manifold only Setup Figures la and 1b below illustrate two configurations wherein the perfusion system operates in either a stopped flow or continuous flow to waste mode In general the shortest response time of the system to selec tion of a solution will be achieved by keeping the tubing length between the MANIFOLD and sample as short as possible 4f Figure 1a Syringe M Stopcock _ Tygon Tubing Tube to Tube connector pa C Flex tubing Pinch Valve Normally Open Side Su to Tube connector Normally Closed Side Tygon tubing PE 160 Tubing e Fits inside Tygon tubing Manifold Publication 5710 001 REV B Luer Female to Barb Connector I Normally Closed Side Figure 1b Syringe Stopcock Luer Female to Barb Connector Tygon Tubing Y Connector C Flex tubing Pinch Valve Normally Open Side Tube to Tube connector Tygon tubing This line flows to waste PE 160 Tubing a Fits inside Tygon tubing Manifold 2 Perfusion Valve Controller Model VC 6 Warn
5. cts on valve end and 15 pin male D type connector on controller end 60 cc capacity syringes Delrin Holds six syringes with thumb screws for each reservoir Manual Off or External 5 V TTL compatible BNC Connector Logic level pulse 500 ms nominal rear panel BNC connector 2 1 4 1 or 6 1 nominal dead space 110 130 or 200 250 VAC single phase 50 60 Hz 20 watt 10 40 C 50 104 F 89 x 203 x 305 mm 3 5 x 8 0 x 12 in 3 7 kg 8 lb 4 6 kg 10 Ib
6. er Instruments Operating Directions conta The VC 6 Valve Controller is initially assembled by first attaching the VALVE BRACKET to the RING STAND as shown in Figure 2 below This is followed by insertion of C Flex tubing into the pinch valves Depending on the flow arrangement you re using cut six 6 or twelve 12 pieces of C Flex tubing to length Attach a tube to tube connector to each end if using the system shown in Figure 1a Attach a tube to tube connector to only the lower end if using the system shown in Figure 1b Mount the tubing to the valves as shown in Figure 1 NOTES 1 A slight stretch of the tubing while mounting will facilitate insertion into the valve Keep the length of C Flex tubing as short as is feasible 2 The solenoid valves will not transition between open and closed states prior to insertion of the C Flex tubing syringes syringe holder Tygon tubing C Flex tubing valve bracket valve controller ring stand Next attach the SYRINGE HOLDER to the RING STAND Affix a stopcock with Luer connector to each syringe and mount into the SYRINGE HOLDER Now cut six 6 pieces of Tygon tubing just long enough to run from the stopcock to the C Flex tubing Attach a Luer to female barb connector to the top end and a tube to tube connector to the valve end and make attach ments to the syringe and for each channel Finally attach a tube to tube connector to the C Flex tubing on the out board e
7. nd of the valve and attach a short piece of Tygon tubing Insert a short length of PE 160 tubing into the Tygon tubing and make a connec tion to the manifold Publication 5710 001 REV B s Perfusion Valve Controller Model VC 6 Warner Instruments Operating Directions conta AC Conversion ATTENTION PLEASE READ BEFORE APPLYING POWER TO YOUR UNIT The unit has been set to be used with 120 VAC If the VAC needs to be changed to 220VAC enclosed you will find a kit power cord 1 and fuses 2 to be used to convert the unit from 120VAC to 220 VAC The unit uses only one fuse the second one is sent as spare Follow these instructions to change the unit s from 120VAC or 220VAC Power Entry Module Fuse holder side view Push out drawer to access N the Spare Fuse i Active Fuse Step 1 Depending on AC Voltage being used turn VAC selector switch to 110VAC or 220 VAC Publication 5710 001 REV B Step 2 Carefully pry open the fuse holder from the inside by using a small flat screwdriver Replace fuse according to voltage being used Item 4630130 Model VC 6 and VC 6M use For 120 VAC 0 50 Amp 5x20mm Slow Blow For 220 VAC 0 25 Amp 5x20mm Slow Blow 2 Perfusion Valve Controller Model VC 6 Warner Instruments Operating Directions Instructions for Use Flow Adjustment Flow rates c
8. rolled via a manual switch or an external digital TTD signal An event marker pulse generated each time a valve is switched on is provided at the rear of the instrument for recording into your acquisition system Valve transitions opened or closed occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions Pinch valves are supplied as standard equipment They are the simplest from a maintenance standpoint since solutions do not come into contact with the valve and the tubing is easily exchanged Pinch valves are dual acting with both normally open and normally closed sides If the valve input line is split using a Yconnector solution can flow to waste while the valve is in the off position These pinch valves are designed to work with silicone type tubes C Flex or other similar type tubes All systems are supplied with a small quantity of C Flex tubing Response times for the valves varies with solution viscosity and tubing however typical times are 20 50 ms NOTE Minimizing the length of C Flex or silicone tubing used will reduce the gas permeability of the system Publication 5710 001 REV B s Perfusion Valve Controller Model VC 6 Warner Instruments Operating Directions Before beginning setup take inventory of the plastic fittings supplied with the complete system These fittings include six 6 each of 1 16 ID tube to tube conne
9. sembly it should be cleaned as soon as possible with a soft cloth dampened with a mild solution of detergent and water The exterior of this instrument may be cleaned periodically to remove dust grease and other contamination It is not necessary to clean the inside Use a soft cloth dampened with a mild solution of detergent and water and avoid abrasive cleaners Publication 5710 001 REV B gt Perfusion Valve Controller Model VC 6 Warner Instruments Warranty The VC 6 is warranted to be free from defects in materials and workman ship for a period of two years from the date of shipment If a failure occurs within this period we will repair or replace the faulty component s at our discretion This warranty does not cover failure or damage caused by phys ical abuse or electrical stress e g exceeding specified input limits Shipping charges to the factory are the customer s responsibility Return shipping of the repaired unit will be paid by Warner Instruments Inc Specifications Valves Tubing Valve Bracket Connection Cable Reservoirs Reservoir Holder Valve Controller Switch Selection External Input Event Marker Manifold Power Operating Temperature Dimensions H x W x D Weight Shipping Weight Publication 5710 001 REV B 12 VDC 0 25 A to maintain pinch 1 32 ID x 3 32 OD tubing Delrin Mounts on 3 8 or 1 2 ring stand 2 4 meter 8 ft connecting cable terminated with quick disconne
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