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The LONESTAR System User Manual

1. l l al 0 5 0 0 5 1 Field Intensity o The required field intensity can be set by dragging the slide bar within the Field Intensity box to the Compensation Yoltage Logging To 7 NOT LOGGING a 4 4 _ a uge n h uU M e l l l l l EE vo vero I I l 1 1 1 1 1 I I l I I I I I I i z0 30 4 50 6 0 70 J0 100 110 iz 130 140 150 bo oO 180 150 z sert Alarm State Switch to Ton current row 4 U i 0 25 O 2 Bis 0 25 Figure 24 Continuous Mode required value If required the field intensity can also be changed whilst data is being gathered Once the pressure and flow have been set and are stable click on the green Start button to begin gathering data A pop up window will appear into which you can enter a filename for the data clicking on OK will begin the run The Logging To text box will be updated and display the currently defined filename 2010 OWLSTONE Ltd Proprietary and Confidential Page 23 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc 12 07 10 OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 If the Cancel button is clicked instead of the OK button the run will still start but the data will not be saved To stop the run in this instance click on the red Cancel button System Status The system status screen displays active detailed information about the LONESTAR syst
2. CD and ultra violet radiation UV A comparison of ionization FOUTE TA hanya OF ON 2 pecs a l set CV sources is presented in Table 1 Ni 63 undergoes beta decay generating energetic electrons where as CD ionization strips electrons from the surface of a metallic structure under the influence of a strong electric field The electrons generated interact with the carrier gas air to form stable intermediate ions called reactive ion peak RIP with positive and negative charges These RIP ions then transfer their charge to neutral molecules through collisions thus both Ni 63 and CD are known as indirect ionization methods lonisation Source Mechanism Chemical Selectivity Nie beta emitter creates a positive negative RIP Charge transfer Proton electron affinity UV Photons Direct ionisation First ionisation potential Corona discharge plasma creates a positive negative RIP Table 1 FAIMS ionisation source comparison Charge transfer Proton electron affinity For the positive ion formation N e gt No e primary e secondary N 2N gt Na N Nat H2O gt 2N gt F H O H O H2O H 0 OH H30 HO N H H20 2 N H H20 H2O N H H20 N For the negative ion formation O te Oz B H2O O O H20 B B H O F O2 H20 lt gt O H20 2 B 2010 OWLSTONE Ltd Proprietary and Confidential Page 43 of 48 The Owlstone logo OWLSTONE and LONESTAR are tradema
3. PTFE gasket 2010 OWLSTONE Ltd Proprietary and Confidential Page 31 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Before removing the external filter assembly the following two steps must be followed 1 Stop gathering data by clicking on the Stop button This can be found on the main navigation bar in the LONESTAR system software 2 Next switch the Filter Heater off and allow the filter body to cool The filter heater is turned off by clicking on the Filter Heater button which can be found on the Settings tab Once this is done the temperature can be monitored on the Status screen Instrument Set Up OFF ON AUTO Pump O OFF Gas Purge CON Sensor Heater On m Filter Heater Figure 31 Filter Heater option The filter body will be extremely hot and should not be handled until it has cooled 3 Whilst the filter body is cooling switch the LONESTAR system gas flow into Purge mode This is done by clicking the Gas Purge button which can be found on the Settings tab Doing this will redirect the gas flow so that it flows out of the sample inlet preventing any particulates being able to enter the LONESTAR system whilst the filter has been removed 4 When the filter has cooled remove the filter assembly Using a 5 8 inch spanner on the outlet body part 1 Figure 29 turn the filter anticlockwise until it can be re
4. Before handling the Hydrocarbon refill please consult the Carbon Refill Material Safety Data Sheet MSDS supplied 1 Begin by removing the scrubber from the LONESTAR instrument by pressing down on the scrubber cartridge release Figure 35 Scrubber removal 2 Next unscrew the scrubber cap in an anticlockwise direction and remove it from the scrubber body 2010 OWLSTONE Ltd Proprietary and Confidential Page 35 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Scrubber cap Scrubber body YAL st lt eee A len ea o o r 3k ae Sie ae iba Ka PN g6 y gt aA Pet seats er gt Hae rig Figure 36 Scrubber cartridge 3 Pour the hydrocarbon media in the lower section of the scrubber into a container taking the necessary precautions detailed in the MSDS 4 Refill the lower section of the scrubber with the new hydrocarbon media up to the internal fill line approximately 3 cm from the top edge of the scrubber body 5 Ensure that the hydrocarbon particulates have settled by gently tapping the scrubber body Top up the hydrocarbon media if the level has dropped 6 Finally screw the scrubber cap back onto the scrubber body until tight Ensure that the o ring seal between the cap and body is still intact after tightening Any damage to this o ring could cause a leak to occur If the o ring is damaged
5. Compensation Yoltage Figure 16 DF Matrix review The DF Matrix for positive and negative mode responses is displayed at the top of the screen The ion current at a single DF sweep can be seen in the graphical view below the positive and negative mode DF Matrix images The ion current can be rescaled so that more detail can be seen in the DF Matrix This is done by adjusting the Rescale slide bars to the right of the DF Matrix Review DF Matrix File 100317 File ee i pa Positive Mode DF Matrix OFF e Smoothing Negative Mode DF Matrix OFFA Smoothing o 90 0 90 0 80 0 w Pi FADE Ds S 60 0 5 50 0 2 40 0 Ww z 30 0 I l l I l l 4 0 2 0 0 0 2 0 4 0 6 0 i 6 4 2 0 0 0 2 0 4 0 Compensation oltage 3 Compenstation oltage DF cursor 63 67 DF cursor 63 66 Positive Mode C Sweep Negative Mode CY Sweep 0 7 A A 0 02 0 6 ay 0 01 0 5 0 4 0 3 0 2 0 1 0 0 1 ie 0 01 0 02 0 03 0 04 0 05 0 06 G a Ion Current A U Ion Current A U 3 Compensation oltage Figure 17 Rescaled DF Matrix 2010 OWLSTONE Ltd Proprietary and Confidential Page 19 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 The ion current at a single DF sweep can be seen in the graphical view below the positive and negative mode DF Mat
6. Low Voltage Directive EN 61010 1 2001 Changes that you make to your system may void compliance with one or more of these EMC and safety standards Changes to your system include replacing a part or adding components options or peripherals not specifically authorized and qualified by OWLSTONE Ltd To ensure continued compliance with EMC and safety standards replacement parts and additional components options and peripherals must be ordered from OWLSTONE Ltd or one of its authorized representatives 2010 OWLSTONE Ltd Proprietary and Confidential Page 4 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 e FCC Compliance Statement This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area Is likely to cause harmful interference in which case the user will be required to correct the interference at his or her own expense Safety Notice Caution Read these operating
7. Proprietary and Confidential Page 29 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc 12 07 10 OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN Maintenance Basic checks e Regularly check that the main LED indicator glows when power is applied The unit must NOT be operated if the indicator fails and must be returned for servicing The fuse in the mains cable plug must only be replaced with a 5A BS1362 type UK only e f a replacement fuse fails immediately contact your local service agent DO NOT replace with a higher rated fuse e The mains adaptors are designed to comply with BS EN 60950 1 and can be flash tested Do not attempt any maintenance whilst the unit is powered on or connected to any gas lines 2010 OWLSTONE Ltd Proprietary and Confidential Page 30 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 External Filter Every precaution should be taken to keep the filter parts clean It is recommended that gloves are worn when handling the filter assembly Any contact with fingers can leave greasy deposits which will cause contamination Figure 29 External filter assembly Figure 30 Filter components 1 Outlet body 5 Stainless steel screen 2 Locking ring 6 PTFE filter membrane 3 Inlet body 7 Stainless steel screen 4 PTFE gasket 8
8. and LOCATION ccccseeseeeeeeseeeeeees 9 EXTERNAL FILTER c cssssseeeeeeeeeceesseeeees 31 Befor SLANG csocsi nnna 10 AES sacaissice sea vnce uc eeene nea vencuescenseusssven versenseaens 10 SE Peg TODE Taa Exchanging the filter cartridge 00000 37 THE LONESTAR SYSTEM SOFTWARE GUIDE TERE E E E tees isco E A N 11 CASEWORK 38 Starting the SOftWALE cccceeessssseeeeeeeeeeeees 11 SERVICING assesses sinsisiceceteavsciaisneececesseciacetedences 39 User interface Orientation ccccccsssseeseeeseeees 11 DF SWCD dala sssr ninnaa a 13 TROUBLESHOOTING sssssrreerrrererrreeerreseerrenees 40 Reviewing DF Matrix files sceeeeeeee 18 TECHNICAL SPECIFICATION 000000 41 Exporting a single DF Matrix 0000 20 Exporting a complete directory D4 TECHNOLOGY AT A GLANCE 000000 42 Configuring and gathering continuous mode Carrier GaS eigenen cetcee ca tvenveesceteneceeescanaeaeesees 43 dala ais 22 Mobility ccccceeeeesseeeeeeececeeesnseeeeeeeeceensneeeeees 45 System Status ccc eecseeeeeeeeeseeeeeceesseeeseeeenneees 24 pete ee Detection and Identification cccceseseeee 46 FH Momi an ctw asa ceansntesedeecundecaccuent convusetesarts 26 BUI GAS aces ane deecisaeecicecepnncicnewesuasecucceevesaceveeceroavees 26 2010 OWLSTONE Ltd Proprietary and Confidential Page 2 of 48 Th
9. differential mobility spectrometry DMS is a gas detection technology that separates and identifies chemical ions based on their mobility under a varying electric field at atmospheric pressure Figure 40 is a schematic illustrating the operating principles of FAIMS RF waveform Ee EN eee eel SLEEN DEON EENE EN EE ov re PkV lonisation source Lf lon count r eae LA B E ey o y H Detector Sample lonization lon separation based on mobility Detection Exhaust Preparationand introduction Air carrier gas flow direction Figure 40 FAIMS schematic The sample in the vapor phase is introduced via a carrier gas to the ionisation region where the components are ionised via a charge transfer process or by direct ionisation dependent on the ionisation source used It is important to note that both positive and negative ions are formed The ion cloud enters the electrode channel where an RF waveform is applied to create a varying electric field under which the ions follow different trajectories dependent on the ions intrinsic mobility parameter A DC voltage compensation voltage is Swept across the electrode channel shifting the trajectories so different ions reach the detector Which simultaneously detects both positive and negative ions The number of ions detected is proportional to the concentration of the chemical in the sample FAIMS can be used to detect volatiles in aqueous solid and gaseous matrices and
10. field z axis The plot on the right is cv the one that is more frequently used and is referred Figure 47 Example CV spectra Six different to as a 2D color plot The compensation voltage chemical species with different mobilities are pd electric field are on the x and y axes and the filtered through the electrode channel by 6n current is represented by the color contours scanning the CV value P y lon current 2010 OWLSTONE Ltd Proprietary and Confidential Page 46 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Electric Field mi Jom Coron ATi ugga Tampoi Yaiergs Compensation voltage Figure 48 Two different examples of FAIMS dispersion field matrices with the same reactive ion peaks RIP and product ion peaks PIP In the waterfall plot on the left the z axis is the ion current this is replaced in the right more frequently used colorplot by color contours With these data rich DF matrices a chemical fingerprint is formed in which identification parameters for different chemical species can be extracted processed and stored Figure 49 shows one example here the CV value at the peak maximum at each of the different electric field settings has been extracted and plotted to be later used as a reference to identify the same chemicals In Figure 50 a new sample spectrum has been compared to the reference
11. in the hardware manual supplied with the LONESTAR system e Motherboard An indication of the operating temperature of the LONESTAR motherboard e Ambient The ambient temperature within the LONESTAR body All of these parameters can be plotted using the dual axis graph feature displayed on the right hand side of the screen Flow Rate Limin w Inline Temperature C w z 54 55 fe ae Sensor Temperature Motherboard Temperature C 69 41 68 40 Figure 26 Dual Graph To display a parameter on the graph click on the drop down menu and select the one required The scales will be automatically adjusted 2010 OWLSTONE Ltd Proprietary and Confidential Page 25 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 File Menu The File menu item provides access to the Configuration menu The configuration feature allows you to create a system configuration which can then be saved a reloaded multiple times This saves time when setting up the system and ensures that a consistent system set up is applied each time data is collected Three configuration options are available e New Configuration e Load Configuration e Save Configuration To access these menu items click on File in the toolbar and then choose the required option Plugins Help New Configuration l Stat Load Configuration Save Configuration ion Exit
12. instructions fully before use and pay particular attention to sections containing this symbol Always observe the following safety precautions Only connect to an earthed supply socket THE MAINS ADAPTOR IS CLASS 1 CONSTRUCTION AND MUST BE EARTHED GROUNDED Ensure the mains outlet is easily reached to disconnect the unit Use only the supplied mains adaptor and leads supplied The equipment is heavy use the handle provided to lift and carry the unit Always disconnect the equipment from the mains supply before moving This equipment is for use in moderate climates only NEVER use the equipment in damp or wet conditions Avoid excessive heat humidity dust amp vibration Do not use where the equipment may be subjected to dripping or splashing liquids Harmful substances The LONESTAR system can be used with a wide range of samples some of which could be toxic or harmful The LONESTAR system is not a fully sealed unit therefore for this reason it is essential that the user conduct a risk assessment for the substances to be used in the LONESTAR and establish safety protocols to cope with the release of such materials under the normal operation of the unit These protocols must include suitable installation e g in a fume cupboard provision of extraction etc and operational procedures to protect the operator The LONESTAR system is not designed to be a fully sealed unit Therefore when used with any toxic or harmful compound appropri
13. ir 4 Mi T ere r Ion Current A U Compensation Yoltage Compensation Yoltage Figure 12 DF screen Before the run can be started the system pressure and gas flow must be set by pressurising the LONESTAR system and adjusting the exhaust restriction until the desired flow is reached The optimal pressure and flow settings can be found in the application notes supplied with your instrument Once the pressure and flow have been set and are stable click on the green Start button to begin gathering data A pop up window will appear into which you can enter the filename of the DF Matrix file clicking on OK will begin the run The Logging To text box will be updated and display the currently defined filename 2010 OWLSTONE Ltd Proprietary and Confidential Page 16 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 If required a prefix can be entered that will be appended to the original filename This must be entered into the Prefix text box on the screen before the DF Matrix file has reached is end value If the Cancel button is clicked instead of the OK button the run will still start but the data will not be saved To stop the run in this instance click on the red Cancel button As the run begins you will begin to see the DF Matrix being constructed in both the positive and negative mode areas File P
14. it must be replaced 2010 OWLSTONE Ltd Proprietary and Confidential Page 36 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 O ring seal Figure 37 O ring seal Before disposing of any used hydrocarbon media please read the Hydrocarbon MSDS supplied with the LONESTAR system Exchanging the filter cartridge 1 Begin by removing the scrubber from the LONESTAR instrument by pressing down on the scrubber cartridge release Figure 35 2 Next unscrew the Scrubber cap in an anticlockwise direction and remove it from the scrubber body 3 Finally unscrew the filter cartridge assembly by holding onto the cylinder cap and turning the filter assembly in an anticlockwise direction Filter SER i cartridge y assembly Scrubber cap Figure 38 Filter Assembly 2010 OWLSTONE Ltd Proprietary and Confidential Page 37 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 4 Screw the new filter cartridge assembly into the scrubber cap taking care not to over tighten the threads 5 Finally screw the scrubber cap back onto the scrubber body until tight Ensure that the o ring seal between the cap and body is still intact after tightening Any damage to this o ring could cause a leak to occur If the o ring is damaged it must be replac
15. of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 System Optimization Due to the versatility and dynamics of the LONESTAR system there may be some instances when the spectral output can become crowded and difficult to read This is a result of many variables but various adjustments can be made to optimize the spectral output No Peak If a system produces no peak at all a number of issues could be the cause and can show itself in a number of ways which are listed below e When running the system at 0 DF if there is simply a noise line at or around 0 au in height with no sign of a peak this indicates there are no ions passing through the chip to the detector To rectify first check the main pump or regulator is on and flow is present in the system Second check whether the mode of operation requires some fixtures to be removed or replaced In an extreme situation the particulate filter could be blocked and may need replacing e Sometimes when running at 0 DF there is a peak but when running at elevated DF values the peaks are no longer observable This is due to ion attenuation and is a perfectly normal phenomenon related to the size of the sample analyte passing through the device and its concentration Large molecular ions will give a response over the full range of DF values whereas small ions will only have a short range To overcome these problems try focussing the DF value from 25 to 45 in
16. order to observe the smaller ions e When running the system at any DF value if the response is a flat line at or around 10 au then an OWLSTONE technician should be contacted This may indicate that the FAIMS chip is damaged although this is extremely rare System saturation this occurs when there too many ions striking the detector plate and as a result the peak s may have the top chopped off or clipped this is caused by the ion count overwhelming the detecting electronics Another example of saturation can be observed from peak height and width if either of these are excessively large then saturation has occurred e Clipping When running the system at 0 DF with no sample flow if the only peak is clipped then the flow rate is too high The needle valve on the LONESTAR exhaust controls the flow rate and this should be adjusted until the correct flow is displayed in the software The pressure within the LONESTAR will also change as the flow is decreased check the pressure reading and adjust to the required set point Clipping When running the system at 0 DF with the sample flow on if the only peak present is clipped this may indicate a very high concentration of sample vapour entering the system Check the sample and reduce to the lowest setting lowest flow in steps until the signal no longer clips e Excessive peak size When operating the system at DF values of 30 and above if any peak within the positive se
17. peaks and the new samples About OWLSTONE OWLSTONE is developing and commercializing innovative new technologies to address the critical need for compact dependable and cost effective chemical and biological detection solutions for a wide range of markets We were formed through the recognition of the opportunities created by the application of micro and nano technology to develop improved sensing solutions OWLSTONE is focused on the innovation of detection technologies to address unmet needs We develop solutions that are flexible enough to target a range of markets with the potential for growth by enabling new application opportunities From homeland security to home safety OWLSTONE is working with leading manufacturers and integrators across a range of markets to develop products incorporating our microchip chemical sensing solution OWLSTONE is headquartered in the United States and has laboratory facilities in the United Kingdom We were founded in 2003 with a seed investment of two million dollars from Advance Nanotech Inc a New York based company specializing in the investment in and commercialization of nanotechnologies Contact US Office UK OWLSTONE Nanotech Inc 127 Cambridge Science Park 400 Rella Boulevard Milton Road Suite 160 Cambridge Suffern NY 10901 CB4 OGD Tel 1 845 533 4225 Tel 44 0 1223 428 200 Fax 1 845 533 4232 Fax 44 0 1223 428 201 Email info OWLSTONEnanotech com Web www OWLSTONEnanotec
18. purchaser is exempt from any regulatory requirements For transport purposes the LONESTAR system complies to UN2911 standards for radioactive sources and is provided with an identification certificate High humidity and acidic conditions can potentially damage the Nickel 63 source if used in this manner or if these conditions could potentially occur please consult an OWLSTONE service engineer about the use of a secondary external downstream filter to prevent spread of contaminated material No person shall intentionally or recklessly misuse or without reasonable excuse interfere with the radioactive substance contained within the LONESTAR system The source is enclosed in a non user serviceable housing with tamper seals 2010 OWLSTONE Ltd Proprietary and Confidential Page 6 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Setup Guide System Contents The LONESTAR system is shipped with the following LONESTAR process monitor User guide Radiation source certificate Mains power adaptor Mains power cable Keyboard Peli transport case Accessories pack If any of these items are missing or damaged then contact OWLSTONE Ltd immediately Please retain all items and packaging including the Peli Case which is required to return the unit to OWLSTONE for annual service No responsibility is accepted by OWLSTONE for damage arisin
19. F Matrix Interval sec 142 9 AS oo Field Intensity Min CY Sweep Interval sec Linear Custom Agi Start Average CV Sweeps Number of Lines ey Figure 8 DF Matrix settings The DF Matrix settings options provide the ability to configure your run to focus on all or part of the DF Matrix DF Matrix Settings DF Matrix Length sec Min DF Matrix Interval sec as aye Field Intensity Min CY Sweep Interval sec Linear Custom Ay Start o Average CY Sweeps J Ti i End o 100 Sumber of Lines Figure 9 DF Matrix Settings 2010 OWLSTONE Ltd Proprietary and Confidential Page 14 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Setting the DF Sweep range Linear The range over which the DF sweeps will be gathered can be set by adjusting the set points for the start and end DF values To obtain a full DF Matrix set the start point to 0 and the end point to 100 Once your target analyte has been identified you can adjust these options at a later date to only gather data between two new points over a smaller range e g if your target analyte can be seen at 60 DF you could gather data between 30 and 80 instead Setting the DF Sweep range Custom Rather than gathering DF sweeps linearly it is possible to configure the software to gather D
20. F sweeps at custom intervals Start by clicking on the Custom tab under the Field Intensity area Field Intensity Linear Custom The Custom tab allows Field Intensity values to be entered into the cells io W These values will be used by the LONESTAR system to gather a series of CV sweeps at those defined values To enter values click inside the next available cell and enter the required Field Intensity Repeat this until all the required values have been entered SohjeA Figure 10 Custom Field Intensity Field Intensity Linear Custom o E 10 Once these values have been entered they will be used when gathering 20 DF Matrix files To return to gathering data using the linear configuration 50 click on the Linear tab button 70 p Fa 75 E 100 o Figure 11 Custom Field Intensity Setting the Number of Lines The number of lines selected will affect the resolution of the DF Matrix A commonly used rule is to divide the DF range by 2 and add 1 however any number of lines can be chosen In Figure 9 the number of lines is set to 51 for a range of 0 to 100 The number of lines also affects the time taken to complete a full DF Matrix Average CV Sweeps The number of averages for each DF sweep can be changed for increased resolution of the peaks However this will increase the run time for each DF sweep and the overall time taken to gather 1 DF Matrix Min DF Matrix Interval sec Setting this value p
21. OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 The LONESTAR System User Manual 2010 OWLSTONE Ltd Proprietary and Confidential Page 1 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 About this Manual This user manual contains all the information you will need to initially install and run the OWLSTONE LONESTAR process chemical monitoring system Additional information and updates are available on the owlstonenanotech com website under the support tag It is essential that this user manual be read and understood before commencing any work with the system Read and understand the various precautionary notes signs and symbols contained inside this manual pertaining to the safe use and operation of this product before using the device Using the LONESTAR system in a way that is not specified in this manual could be harmful to health of the operator and co workers This symbol is used to highlight a section explaining particularly important safety considerations Contents NOTICES smirim 3 SYSTEM OPTIMIZATION ssccseeeeeeeeees 27 SETUP GUIDE snennnnnnonnunnnoennnnnnonnnnnnnnnnnnnnennnnnnen 7 EXPANSION PORTS ccccssssseeeeseseseeeseeeees 29 System Contents ccccceeeeeeesesssseeeeeeeeeeeeenenes 7 MAINTENANCE ococcccocccocccecccccceccccceecececccc 30 Installation
22. Prefix Figure 27 Configurations Configuration Options e New Configuration Clicking on this option will clear the existing configuration being used on the LONESTAR system and reload the system default configuration file The configuration identifier in the toolbar will be updated to reflect your selected configuration e Load Configuration This option allows you to reload any saved configuration When selected a navigation window will be displayed to locate the required file Once located either double clicking on the configuration file or selecting it and then clicking OK will load it into the LONESTAR system software The configuration identifier in the toolbar will be updated to reflect your selected configuration e Save Configuration Once the LONESTAR system software has been optimized to your requirements you can save the configuration directly from the LONESTAR system software by clicking this option All of your LONESTAR system settings will be exported into a configuration file which can be saved in the chosen Plugins The Plugins menu item provides access to the Matrix review software and Easyspec To access these menu items click on Plugins in the toolbar and then choose the required option DF OCReview tings Stat mums CEas Spec P Lor Calibrate fection Prefix Figure 28 Plugins 2010 OWLSTONE Ltd Proprietary and Confidential Page 26 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks
23. Proprietary and Confidential Page 39 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Troubleshooting Symptom Possible cause remedy Not reaching set flow rate o Filter clogged Change filter o Scrubber restriction Check scrubber o Exhaust restriction Check exhaust o Flow path restriction Contact service engineer o Flow sensor malfunction Contact service engineer System does not boot o Check mains and fuse o Check hard on off switch at back of unit o Other Contact service engineer Sensor or filter temperature o Contact service engineer does not reach set point Humidity not reaching o Faulty humidity sensor contact service engineer desired level o Incorrect scrubber scrubber consumed replace refill scrubber o External air supply is wet Contamination o Dirty sampling interface pipe work Clean pipework o Scrubber may need its contents changing o Leave system running with clean dry air and all heaters on for 1 day o Particulate filter is contaminated and needs replacing USB accessory not o Faulty USB software did not detect device try second USB port detected Software freezes o Reboot by pressing front on off button twice NI error message o Reboot if problem persists please contact service engineer with error message code No background ion peak o Check scrubber is properly mounted
24. ale l 3 4 Peed 6 0 4 0 2 0 0 0 20 4 0 6 0 6 0 4 0 2 0 0 0 2 0 4 0 6 0 15 01 Compenstation Yoltage Compensation oltage Y DF cursor 0 00 DF cursor 0 00 Positive Mode CY Sweep Negative Mode C Sweep Ion Current A U l l l l 1 iy S Ga Compensation oltage Compensation oltage Y ts ooo se Figure 15 Review DF Matrix file To load a DF Matrix file click on the File menu item and click on Load data A Windows search box will be displayed so that you can navigate to the location of the saved files and once selected the DF Matrix will automatically be displayed 2010 OWLSTONE Ltd Proprietary and Confidential Page 18 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Review DF Matrix File 100317 File gg i Se N a Positive Mode DF Matrix OFF Smoothing Negative Mode DF Matrix COFFS Smoothing 90 0 90 0 80 0 Ww 70 0 60 0 I 50 0 2 40 0 E E 30 0 phere UP Q nH 0 0 1 1 1 l l j l j j 6 0 4 0 2 0 0 0 2 0 4 0 6 0 6 4 0 2 0 0 0 2 0 4 0 Compensation Yoltage Compenstation Yoltage DF cursor 63 67 DF cursor 63 66 Positive Mode C Sweep Negative Mode CV Sweep Da BE 0 02 0 6 sE 0 01 0 5 A 0 4 0 01 0a 0 02 0 03 0 04 0 05 0 06 Ion Current A U Ion Current A U i 3
25. arget analytes that exceeds the lower limits of detection of the FAIMS device 2010 OWLSTONE Ltd Proprietary and Confidential Page 42 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Carrier Gas The requirement for a flow of air through the system is twofold Firstly to drive the ions through the electrode channel to the detector plate and secondly to initiate the ionization process necessary for detection As exhibited in Error Reference source not found the transmission factor proportion of ions that make it to the detector increases with increasing flow The higher the transmission factor the higher the sensitivity Higher flow also results in a larger full width half maximum FWHM of the peaks however decreasing the resolution of FAIMS unit see Figure 42 Figure 41 Flow rate vs ion As the air carrier gas determines the baseline reading of the transmission factor instrument Therefore for optimal operation it is desirable for the OF carrier to be free of all impurities lt 0 1 pom methane and the humidity to be kept constant It can be supplied either from a pump or compressor allowing for negative and positive pressure operating modes lonization Source There are three main vapor phase ion sources in use for atmospheric pressure ionization radioactive nickel 63 Ni 63 corona discharge z a aes
26. ate precautions such as operation under a fume hood is required Check chemical compatibility Materials in the flow path include PTFE stainless steel aluminium silicon graphite circuit board and Viton Ensure test atmospheres are neither corrosive nor reactive with materials in the flow path and can be safely operated at 60 C If in doubt please contact an OWLSTONE representative using the contact details provided Note that the relative humidity sensors are for indication only Typically the humidity sensors are accurate to 5 but they can be affected by the exact makeup of the process sample and can be easily altered or damaged by certain chemicals 2010 OWLSTONE Ltd Proprietary and Confidential Page 5 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Radioactive Material The LONESTAR system contains a radiation source please refer to the radiation source materials safety data sheet for more details on safe handling Please consult local regulations about your responsibility in regards to the source For instance in England and Wales this will be compliance with the radioactive substance testing instruments England and Wales exemption order 2006 and the ionising radiation regulations 1999 In the US the LONESTAR system is manufactured in compliance with U S NRC safety criteria in 10 CFR 32 27 so the
27. can consequently be used for a wide variety of applications The user requirements and sample matrix for each application define the sample preparation and introduction steps required There are a wide variety of sample preparation extraction and processing techniques each with their own advantages and disadvantages It is not the scope of this overview to list them all only to highlight that the success of the chosen application will depend heavily on this critical step which can only be defined by the user requirements There are two mechanisms of introducing the sample into the FAIMS unit discrete sampling and continuous sampling With discrete sampling a defined volume of the sample is collected by weighing or by volumetric measurement via a syringe or passed through an adsorbent for pre concentration before it is introduced into the FAIMS unit An example of this would be attaching a sample container to the instrument containing a fixed volume of sample Where a carrier gas usually clean dry air is used to transfer the sample to the ionization region Continuous sampling is where the resultant gaseous sample is continuously purged into the FAIMS unit and either diluted by or acts as the carrier gas For example continuously drawing air from the top of a process vat The one key requirement for all the sample preparation and introduction techniques is the ability to reproducibly generate and introduce a headspace vapor concentration of the t
28. ction of the compensation voltage sweep is wider than 2V at the peak base and over 7 au in height this could be obscuring extra information Either reduce the flow rate as covered in the Clipping instructions above or reduce the sample concentration This can be done by preparing the sample at a lower concentration or splitting off some of the analyte before the LONESTAR sample inlet Turn down the sample wheel in steps until this peak has reduced sufficiently 2010 OWLSTONE Ltd Proprietary and Confidential Page 27 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Peak Mirroring In extreme circumstances the peak in one polarity can be large enough to impact on the opposite polarity spectrum This is due to high ion concentrations of one polarity interfering with the detection electronics Mirroring is only detrimental if there is information in both detection polarities with one polarity overwhelming the other e Mirroring High ion counts can be limited by changing the sample flow through the sample wheel as mentioned in the clipping section and by changing the main pump voltage or flow Minimal Peak Movement All peaks within the spectral window should move left or right as the DF values are increased Very few ions will remain on a straight line in the spectrum such an observation may indicate a problem charged particulates ma
29. d at 34 DF Within the graphical view are 3 cursors red green and blue These can be dragged onto a peak of interest to monitor changes in the peak size position whilst data is being gathered Once all of the required DF Matrix files have been captured click on the red stop button to finish the run 2010 OWLSTONE Ltd Proprietary and Confidential Page 17 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Reviewing DF Matrix files DF Matrix files that have been saved can be viewed at any time even whilst the LONESTAR system is gathering data This feature is called Review DF Matrix files and is accessed by clicking on the Plugins menu item then selecting Review and finally clicking on DF Matrix file File Help Configuration Default Review Process eee File ntinuous M l Easy5pec gt VE Mahri E 3 ormar ae 0 0 Gas Flow L min o gt Hele Warn J E Lor Calibrate nt Settings Alarm G 0 00 Pressure barg Negative STAR Figure 14 Selecting review DF Matrix file The Review DF Matrix file window will be displayed Review DF Matrix File 100317 File Positive Mode DF Matrix COFF me Smoothing Negative Mode DF Matrix OFF Smoothing 100 0 100 0 30 0 30 0 50 0 50 0 70 0 70 0 60 0 60 0 50 0 g4 6 6 50 0 Dispersion Field o full scale Dispersion Field o full sc
30. different concentrations Under the action of the indirect ionization process is the manner in which ions are formed is exactly the same however the likelihood of ionization is governed by its affinity towards proton and electrons Table 2 and Table 3 respectivley In complex mixtures where more than one chemical is present competition for the available charge occurs resulting in preferential ionization of the compounds within the sample Thus the chemicals with high proton or electron affinities will ionize more readily than those with a low proton or electron affinity Therefore the concentration of water within the ionization region will have a direct effect on certain analytes whose proton electron affinities are lower Chemical Family Example _ Proton affinity Aromatic amines Pyridine 930 kJ mole Amines Methly amine 899 kJ mole Phosphorous TEP 891 kJ mole Compounds Sulfoxides DMS 884 kJ mole Ketones 2 pentanone 832 kJ mole Esters Methly Acetate 822 kJ mole Alkenes 1 Hexene 805 kJ mole Alcohols Butanol 789 kJ mole Aromatics Benzene 750 kJ mole Alkanes Methane 544 kJ mole Table 2 Overview of the proton affinity of different chemical families 2010 OWLSTONE Ltd Proprietary and Confidential Page 44 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Chemical Family Electron affinity Organomercurials Pesticid
31. e Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Copyright 2010 OWLSTONE Ltd All rights reserved OWLSTONE Ltd provides this user manual to its customers to use in the Product operation This manual is copyright protected and no part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language or computer language in any form or by any means without the prior written permission of OWLSTONE Ltd The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc Swagelok is a registered trademark of Swagelok Company Microsoft and Windows are registered trademarks of the Microsoft Corporation and the embedded Windows software must be used in accordance with Microsofts terms and conditions see www microsoft com Disclaimer OWLSTONE Ltd makes no representations or warranties either expressed or implied with respect to the contents hereof and specifically disclaims any warranties merchantability or fitness for any particular purpose Furthermore OWLSTONE Ltd reserves the right to revise this publication and to make changes from time to time in the contents hereof without obligation of OWLSTONE Ltd to notify any person of such revision or changes Notice of Proper Use of OWLSTONE Ltd Instruments The supplied system is in compliance with international reg
32. ed O ring seal Figure 39 O ring seal Casework Wipe the casework with a damp cloth only Do not wet or allow moisture to penetrate the unit Do not use solvents 2010 OWLSTONE Ltd Proprietary and Confidential Page 38 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Servicing It is highly recommended that the LONESTAR system undergoes an annual service as well as retesting and calibrating to factory acceptance standards At the same time the unit will undergo a wipe test to ensure no radioactive contamination has escaped the housing In some regions this is a regulatory requirement and a Certificate of Conformance will be supplied at each service PLEASE NOTE Opening the electrical case of the product voids the warranty To arrange a service use the OWLSTONE website to obtain an RMA Return Merchandise Authorization number and transport address Alternatively contact OWLSTONE on the service number 44 0 845 838 9866 or at Supportowlstone co uk Do not send a LONESTAR system back to OWLSTONE without first obtaining an RMA and filling in the decontamination certificate provided with the RMA For legal and health and safety reasons OWLSTONE cannot accept any LONESTAR system without the correct paperwork The LONESTAR system will only be accepted if returned in its original Peli case 2010 OWLSTONE Ltd
33. efer to the troubleshooting guide in the application notes e Humidity Shows the relative humidity of the gas flowing through the LONESTAR system e Temperature This temperature must have stabilized or be near the maximum reading that can be achieved This parameter can also be graphed within this screen to show the trend of the data i e has it stabilized or is it still climbing e Field Intensity Updates as the DF Matrix is constructed 2010 OWLSTONE Ltd Proprietary and Confidential Page 24 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 System Temperatures The next set of variables is the System temperatures These are still important to the operation of the LONESTAR system and should be checked to ensure that they have reached the maximum stable operating temperature e Sensor Head This is the current operating temperature of the sensor head This must have reached or be near its maximum stable temperature By default the sensor head should reach 60 C 2 If the temperature is not reaching this range then consult the troubleshooting guide provided in the hardware manual supplied with the LONESTAR system e Filter The inlet filter temperature must be operating near or at its maximum temperature The set point is 100 C 2 If the temperature is not reaching this range then consult the troubleshooting guide
34. em s key system variables Monitoring and knowing these variables is vital to gathering stable and repeatable data To display the System Status screen click on the Status tab Sensor Conditions Inline Temperature w 54 Flow Rate Pressure Humidity Temperature Field Intensity e a2 System Temperatures 1 a8 er Sensor Temperature C Motherboard Temperature C Sensor Head BB C Filter Boel PE Motherboard 40 9 C Ambient __ 347 C Pump Woltage WH 5g Configuration Default 65 40 ScrubberUsed B FAIMS Sensor Running Last Cal 00 00 00 DEMM Y Filter Used J Figure 25 System Status The left hand side of the screen shows simple spark lines with the last recorded value Sensor conditions The sensor conditions are critical to gathering data and need to be fully understood Each of them will have an impact on the quality of the data and should be regularly checked e Flow Rate Displays the gas flow through the LONESTAR system in litres min A stable flow must be achieved before data should be gathered If the flow is unstable or does not reach the required set point then refer to the troubleshooting guide in the application notes e Pressure Displays the pressure within the LONESTAR system in bar A stable pressure must be achieved before data should be gathered If the pressure is unstable or does not reach the required set point then r
35. es Nitro compounds Halogenated compounds Oxygen 0 45eV Aliphatic alchohols Poo Table 3 Relative electron affinities of several families of compounds The UV ionization source is a direct ionization method whereby photons are emitted at energies of 9 6 10 2 10 6 11 2 and 11 8eV and can only ionize chemical species with a first ionization potential less than the emitted energy Important points to note are that there is no positive mode RIP present when using this ionization source and also using UV ionization is very selective for certain compounds Mobility lons in air under an electric field will move at a constant velocity proportional to the electric field Where the proportionality constant is known as mobility Referring to Figure 44 as the ions enter the electrode channel the applied RF voltages create oscillating regions of high Vyr and low Vpp electric fields as the ions move through the channel The difference in the ion s mobility at the high and low electric field regimes dictates the ion s trajectory through the channel This phenomenon is referred Differencein mobility as differential mobility Figure 44 Schematic of a FAIMS The physical parameters of a chemical ion that affects its Channel showing the difference in the differential mobility are its collisional cross section and ability 0NS trajectories caused by their to form clusters within the high low regions The different mobilitie
36. filter Finally place the second PTFE gasket onto the stainless steel screen 2010 OWLSTONE Ltd Proprietary and Confidential Page 33 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 10 Before fitting the filter assembly back together check that the Locking Ring gasket is fitted into the Inlet body as shown in Figure 33 Locking ring gasket Figure 33 Locking ring gasket 11 The next step is to fit the filter bodies back together and tighten up the locking ring yy Figure 34 Final assembly 12 Remove the old PTFE tape from the fittings and replace it with new PTFE tape Ensure that there are no threads of PTFE tape within the flow path 13 Finally the external filter can be fitted back onto the LONESTAR system Before fitting the filter remove any strands of PTFE sealing tape from the LONESTAR system inlet port threads Check that the outlet body thread has sufficient PTFE sealant tape around the thread and then screw the filter body in until it is tight 2010 OWLSTONE Ltd Proprietary and Confidential Page 34 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Scrubber Cartridge Before removing the scrubber cylinder ensure that the gas supply to the LONESTAR system has been switched off
37. formation of the currently selected feature of the LONESTAR system software Currently this is showing the default feature selection DF Matrix 2010 OWLSTONE Ltd Proprietary and Confidential Page 11 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Prefix Logging To Positive Mode DF Matrix Negative Mode DF Matrix 100 0 m 100 0 x M 90 0 A 30 0 z a 20 0 E e 20 0 J To A 5 A mh 5 i 5 z 70 0 D z 70 0 m o 00 P o b00 Z 5 500 500 a a 0 1 W 40 0 ni W 40 0 5 4 1 5 o m0 68 R 300 3 1 A 5 1 amp i ww i i amp 20 0 3 4 B 5 101 0 46 a 8 4 0 0 l l l l l l e ae I I I l l I os 6 0 4 0 20 0 0 20 4 0 6 0 6 0 4 0 2 0 0 0 2 0 4 0 60 Compensation Yoltage Compensation Yoltage Positive Mode CY Sweep Negative Mode C Sweep 1 a l l 1 T I I Lt a OO I a Mas 1 1 T I g I I i oS re MeEE gt lt oos cscs scccs states sees coco coors eect cA oe feragy a ee ee eae da l ba i I la 1 I 2 E i Z I i l l l l i l l l l l Compensation Yoltage Navigation toolbar Compensation Yoltage Y Figure 5 System information The navigation toolbar provides access to the LONESTAR system software features and is divided into four sections as shown in Figure 6 Men
38. ftware Begin by loading or displaying the required DF Matrix using the DF Matrix review feature Next select the File menu item and choose Export file from the list Review DF Matrix File 100317 Load Data l Export File DF Matrix DFF Smoothing Export Folder Exit l l l l l 6 0 4 0 2 0 0 0 2 0 4 0 6 0 Compensation Yoltage Figure 19 Exporting a File 2010 OWLSTONE Ltd Proprietary and Confidential Page 20 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 A window will be displayed so that the location of the exported file can be set Navigate to the required location and click on OK Export To Spreadsheet File Save in E Mew Folder 2 d 4 My Recent ocuments Desktop My Documents My Computer ey My Network File name export_matrix_1 r Places i Save as Ippe ustom Pattern txt Cancel The exported file will be created in the specified location This can be repeated multiple times for single DF Matrix files Exporting a complete directory If multiple DF Matrix files need to be exported then this can be done using the Folder Export feature Begin by opening the DF Matrix review tool Next select the File menu item and choose Export file from the list Review DF Matrix File 100317 Load Data Export File Export Folder DE Matrix OFF Be Sm
39. g from the use of non approved packaging Figure 1 Peli Case 2010 OWLSTONE Ltd Proprietary and Confidential Page 7 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Bi my Figure 2 The LONESTAR system 1 Scrubber cartridge 6 External filter 2 Tracker ball 7 Clean gas supply port 3 Right click button 8 Exhaust port 4 Left click button 9 USB port 5 Scrubber cartridge release 10 Power switch 2010 OWLSTONE Ltd Proprietary and Confidential Page 8 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Installation and Location Total un packaged system weighs 7 8kg please take care in handling to avoid injury Before using the system ensure that all power cables are intact with no damaged insulation or frays e Ensure that the LONESTAR instrument is placed on a solid level surface which is able to support its weight e Only use the OWLSTONE supplied power supply e Ensure cabling is routed behind the system at bench level posing no risk of tripping Ensure all cables are detached from the LONESTAR instrument before attempting to move the unit e The LONESTAR instrument gets warm during operation handle with care and ensure there is adequate ventilation around the system e Do not bloc
40. gin configuring the LONESTAR system by clicking on the Settings tab The system information area will be updated with the Settings options screen as shown in Figure 22 DF Matrix Settings Instrument Set Up DF Matrix Length sec Min DF Matrix Interval sec Gas Flow Control 143 9 pe OFF ON AUTO Pump EE AY O O Field Intensity Min C Sweep Interval sec OFF a Gas Purge Linear Custom A y 1 CON Sensor Heater Start Yo Average CY Sweeps J A a CON S Filter Heater End o Number of Lines Commer 51 ag Analyhes Concentration ppb A nps Operator Results Path a Figure 22 System Settings 2010 OWLSTONE Ltd Proprietary and Confidential Page 22 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN Once the Continuous DF settings have been configured click on the Continuous tab in the navigation bar File Plugins Help DF Matrix Settings Status Lonestar Process Monitoring Configuration Default Continuous Normal J E 3 0 00 Gas Flow L min le Warn Alarm GQ 0 00 Pressure barg Figure 23 Continuous Tab Before the run can be started the system pressure and gas flow must be set by pressurizing the LONESTAR system and adjusting the exhaust restriction until the A N desired flow is reached The optimal pressure and flow settings can be found in the application notes supplied with your instrument
41. h com 2010 OWLSTONE Ltd Proprietary and Confidential Page 48 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc
42. high field in between the electrodes the water molecules are forced away from the cluster reducing the size MH this is known as de clustering 2010 OWLSTONE Ltd Proprietary and Confidential Page 45 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 As the low field regime returns so do the water molecules to the cluster thus increasing the ions size clustering thus giving the ion a larger differential mobility Gas density and temperature can also affect the ion s mobility by changing the number of ion molecule collisions and changing the stability of the clusters influencing the amount of clustering and de clustering Changes in the electrode channel s environmental parameters will change the mobility exhibited by the ions Therefore it is advantageous to keep the gas density temperature and humidity constant when building detection algorithms based on an ion s mobility as these factors would need to be corrected for However it should be kept in mind that these parameters can also be optimized to gain greater resolution of the target analyte from the background matrix during the method development process CV 5V N cv 6v Detection and Identification 7 As ions with different mobilities travel down the electrode channel some will have aves trajectories that will result in ion annihilation St Ta agains
43. ive Mode DF Matrix m 100 0 fh 90 0 A 30 0 j e 20 0 J e 20 0 J w A wO A z E F 5 70 0 D z FO 0 n gt 0D BOL 2 5 500 500 a a 0 1 W 40 0 we W 40 0 E 3 4 E wis o m0 68 R 300 3 1 5 1 4 8 ww i i amp 200 3 4 B 5 10 0 ag mon 8 4 00 l l l l l l od LN eo 6 0 40 2 0 0 0 20 4 0 6 0 6 0 4 0 2 0 0 0 2 0 4 0 60 Compensation Yoltage Compensation Yoltage Positive Mode CY Sweep Negative Mode C Sweep S z 5 aa 1 z 5 Sy 1 g i I te E eee nye eee ees ers eee Rae e te Sora aae ee ws SSSSSS SESS SSSS ESSE J i i I 1 I 2 AEs 2 E m ri l l l l l l l l l l l l ri l l l l l l l l l l l l 5 4 3 z 1 O 1 E 3 4 5 5 4 3 il 1 Zz 3 4 5 Compensation Yoltage Compensation Yoltage Y Figure 3 LONESTAR system software User interface orientation The LONESTAR system user interface is divided into two sections Figure 4 shows the Navigation bar This part of the user interface is always displayed for each feature of the software being used File Plugins Help Configuration DF Matrix Settings Status Continuous N l E EB Normal 66 60 Gas Flow L min aay Ide i Warn z 7 Lonestar Data Collection Alarm 0 00 Pressure barg Negative Figure 4 Navigation bar The second section of the user interface is shown Figure 5 This shows system in
44. k ventilation holes e Do not place in space that is poorly ventilated or confined Allow at least 50cm clearance from walls and free flow of air around the system e Do not place near flammable materials 2010 OWLSTONE Ltd Proprietary and Confidential Page 9 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Before starting During standard operating mode air is drawn through the sample inlet and blended with clean air drawn from the atmosphere via the scrubber for further details read sample introduction and clean air guide The sample and blend air then exit the LONESTAR system via the exhaust During operation always apply the following precautions Exhaust The pressure in any gas line connected to the system must not exceed 25psi 1 7 bar Before using the system ensure that all power cables are intact with no damaged insulation or frays Ensure the exhaust end cap is removed before applying flows otherwise the system may be damaged Take care in removing and attaching Swagelok fittings to ensure fingers are not trapped or the instrument is not damaged by over tightening The scrubber material should not be exposed to strong oxidising agents Please read the MSDS for the hydrocarbon scrubber for materials and chemical compatibility Do not operate or adjust any part of the scrubber unit whilst the unit is powered
45. lugins Help Configuration Default DF Matrix Settings Status Ea Normal Q TI ee P Posie MAcquire data E u Warn 9 Alarm 1 30 Pressure barg STOP Prefix Logging To Lonestar Data Collection Positive Mode DF Matrix Negative Mode DF Matrix 90 0 30 0 z 0 0 F 20 0 A z 70 0 z 2 70 0 J lg e lg PE S S 2 60 0 a 60 0 D F E 50 0 1 E 50 0 z m m 2 40 0 ya tte Rs LL Lobe 0 2 2 30 0 be 30 0 en i i 20 0 0 3 amp 20 0 0 8 20 0 5 13 i0 0 10 0 0 2 ila 0 0 l l l i 1 1 0 l l l l l l l l l l l wed 60 4 0 20 0 0 a 4 0 6 0 5 0 40 3 0 2 0 10 0 0 10 20 30 40 50 6 0 Compensation Yoltage Y Compensation Yoltage Positive Mode CY Sweep Negative Mode CY Sweep Z l 1 i 0 5 i I I lg m a E a Leea f f I I 1 0 5 gt 05 i z z eS clas C 2 oe eee ae a Z 0 5 I I I I I ioe I I I l l Ea I I I 7 I I I I I I I I 6 5 4 3 23 1 0 1i 2 3 4 5 6 6 5 4 3 2 1 0 1 2 3 4 5 6 Compensation Yoltage Compensation Yoltage Y Figure 13 Data collection The DF Matrix can be rescaled in both positive and negative modes to increase visibility of the peaks This is done by moving the Rescale lon Current bars either side of the DF Matrix Below the DF Matrix is a graphical representation of the last captured individual DF sweep In Figure 13 these are showing the ion current capture
46. moved from the inlet heater 5 Next undo the Locking ring part 2 Figure 29 This can be undone by hand but occasionally it may be necessary to fit an adjustable spanner to the Locking Ring and a 5 8 inch spanner to the outlet body part 1 Figure 29 and then undo the two parts lt is advisable to separate the two parts over a bench or clean tray as some inner parts may fall out as the pieces are separated 6 Once separated the filter components will be visible as seen in Figure 32 Filter Components Figure 32 Filter components 2010 OWLSTONE Ltd Proprietary and Confidential Page 32 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 7 Next take the outlet body part 1 Figure 29 and gently tap it down onto a bench or hard surface to encourage the filter components to drop out As they are compressed into place sometimes they can be difficult to remove so try to avoid damaging any parts whilst doing this 8 Once the filter components have been removed discard the PTFE membrane part 5 Figure 30 9 Begin to reassemble the filter components into the outlet body in the following order Fit one of the PTFE gaskets into the outlet body Fit one of the stainless steel screens onto the PTFE gasket Place the new PTFE membrane filter onto the stainless steel screen Fit the second stainless steel screen over the PTFE membrane
47. ms complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations When the system is delivered to you it meets all relevant electromagnetic compatibility EMC and safety standards as described in the declaration below OWLSTONE Ltd declares under its responsibility that the electronic product LONESTAR Process Monitoring System is in conformity with the following standards e EMC Directive The LONESTAR system complies with the following standards CR47 2006 Class A Code of Federal Regulations pt 15 Subpart B Radio Frequency Devices unintentional radiators EN61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Group 1 Class B equipment emission section only En1326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Industrial Location Immunity immunity section only EN61000 3 2 200 Electromagnetic compatibility EMC part 3 2 Limits Limits for harmonic current emissions equipment input current up to and including 16A per phase EN61000 3 3 1995 A1 A2 Electromagnetic compatibility EMC Part 3 2 Limits Limitation of voltage changes voltage fluctuations and flicker in public low voltage supply systems for equipment with rated current lt 16A per phase and not subject to conditional connection e Low Voltage Safety Compliance This device complies with
48. o Check for a leak in the flow path For questions and troubleshooting advice please contact us at support owlstone co uk please have the LONESTAR system serial number and current software version available If an internet connection is available it is also possible to enable remote access to the LONESTAR system to assist the troubleshooting process 2010 OWLSTONE Ltd Proprietary and Confidential Page 40 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Technical Specification Input Voltage to main power 115V to 250V 50 60Hz AC adaptor Input Current to main power 1A max at 115V adaptor 0 5A max at 240V Storage Temperature range 30C 60C unit off Operating Temperature range 5C 40C Operating Humidity range 90 RH Weight 7 8kg Front USB Back USB Connectors GPIO port Network port Power connector The mains adaptor is for operation at installation category Il transient voltages and pollution degree Il in accordance with IEC 664 at altitudes up to 2000 metres Mains adaptor qualification 2010 OWLSTONE Ltd Proprietary and Confidential Page 41 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Technology at a Glance Field asymmetric ion mobility spectrometry FAIMS also known as
49. on or connected to any gas lines If positive pressure is being used ensure that the scrubber air vent is always OPEN Do not operate without scrubber unit Do not open the LONESTAR instrument there is a risk of electric shock if covers are removed A separate exhaust line should be made ready to connect to the exhaust outlet of the LONESTAR instrument and the scrubber vent This line should be checked for chemical compatibility and it is recommended that it is exhausted to a hydrocarbon trap or to a fume hood 2010 OWLSTONE Ltd Proprietary and Confidential Page 10 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 The LONESTAR System Software Guide Starting the software The software will load automatically when the LONESTAR system has started up If the software does not appear on your screen double click on the LONESTAR software icon located on the LONESTAR system desktop The LONESTAR software user interface is shown in Figure 3 LONESTAR system software The default behaviour is to always open showing the DF Matrix screen File Plugins Help Configuration DF Matrix Settings Status Continuous N E 3 arma 0 00 Gas Flow L min Bp Positive Idle l Warn J Lonestar Data Collection Alarm Q 0 00 Pressure barg Negative Prefix Logging To Positive Mode DF Matrix 100 0 Negat
50. oothing z bbe E 10 0 z 8 4 6 8 5 1 36 0 l l l l l l i 4 6 0 40 20 00 2 0 4 0 6 00 Mic Compensation Yoltage Y d Figure 21 Export Folder 2010 OWLSTONE Ltd Proprietary and Confidential Page 21 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 A folder export can be carried out without loading a DF Matrix into the DF Matrix review tool It is possible to navigate to the directory containing the DF Matrices and simply export the entire directory without viewing them first Configuring and gathering continuous mode data Continuous DF data differs from DF Matrix data in that it is gathered at one specific DF This allows continuous monitoring of a Known analyte at a constant DF the advantage of this is that it provides a very quick visual indication that the analyte has changed in some way Before Continuous DF data can be gathered the LONESTAR system has to be configured The configuration that was last used will be stored by the LONESTAR system so all of the following steps will not be required each and every time a run is started Different configurations can also be saved and loaded into the software when required A number of key system parameters must be stable before data can be gathered Please refer to the application notes provided with your LONESTAR system about these parameters Be
51. rix images DF cursor 65 67 DF cursor 63 66 Positive Mode C Sweep Negative Mode CY Sweep 0 02 0 01 Ion Current A U Ion Current A U l l l l l l l l l Se D Sp a a il OE o a aa G oo A E i a a l o a a Go ao Compensation Yoltage Compensation Yoltage Y i FREY Figure 18 DF Sweep graph view The graph displays the data at a single DF sweep from the DF Matrix This position is set by moving the cursor within the DF Matrix to the desired point Alternatively the required DF can be manually entered into DF cursor boxes located underneath each of the DF Matrices The graphic view will be automatically updated as the DF cursor position is changed The peak values can be obtained by moving one of the three cursors within the graph view onto a peak of interest This gives the ability to monitor peak size and peak position over time To see a sequence of saved DF Matrix files click on the Next button located at the bottom of the screen The next DF Matrix file will be automatically loaded from the same directory Clicking on the previous button will load the DF Matrix file captured before the last one viewed The DF Matrix review feature also provides the ability to export the DF Matrix files into ASCII file format so that the data can be viewed using spreadsheet or notepad software Exporting a single DF Matrix A single DF Matrix file can be exported when it is displayed in the Review DF Matrix file so
52. rks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 The water based clusters hydronium ions in the positive mode blue and hydrated oxygen ions in the negative mode red are stable ions which form the RIPs When an analyte M enters the RIP ion cloud it can replace one or dependent on the analyte two water molecules to form a monomer ion or dimer ion respectively reducing the number of ions present in the RIP Monomer Dimer H H20 3 M N MH H20 2 No H O lt gt M H H20 No H O Dimer ion formation is dependent on the analyte s affinity to charge and its concentration This is illustrated in Figure 43 using dimethyl methylpohsphonate DMMP In plot A it shows that the RIP decreases with an increase in DMMP concentration as more of the charge is transferred over to the DMMP In addition the monomer ion decreases as dimer formation becomes more favourable at the higher concentrations This is shown more clearly in plot B which plots the peak ion current of both the monomer and dimer at different concentration levels 4 I A B OMMP Mo cenmer peak 4 5 mgm blank response E CHMP Dime Peak 10 Ti yji rei gen 0 ppb gt Swn of Monomer and diner Peak j j mgm 2 Upped m a P j Ha zi Qimgm 20pph lI z per a E La Is Monomer z g 1 0 E A gs F i Compensaion Voltage V DMMP level ppb Figure 43 DMMP Monomer and dimer formation at
53. s experienced at environmental factors within the electrode channel affecting gh and low electric fields the ion s differential mobility are electric field humidity temperature and gas density pressure The electric field in the high low regions is supplied errr eee by the applied RF voltage waveform Figure 45 The frequency of which relates to the number of a Pk to Pk V m A m high low cycles per unit time The duty cycle is the proportion of time spent within each region per e e e m e e e ee m m cycle Increasing the peak to peak voltage bo K increases decreases the electric field experienced asn a l Cycle d t in the high low field regions and therefore Figure 45 Schematic of the idea RF waveform influences the velocity of the ion accordingly It is showing the duty cycle and peak to peak voltage this parameter that has the greatest influence on the differential mobility experienced by the ion lt has been shown that humidity has a direct effect on the differential mobility of certain chemicals by increasing decreasing the collisional cross section of the ion within the respective low high field regions This addition and subtraction of water molecules is referred to as clustering and de clustering Increased humidity also increases the number of water molecules involved in a cluster MH H20 2 formed in the ionization region When this cluster experiences the
54. spectrum and clear differences in both spectra can be seen Positive Mode DF Matrix Linear 100 90 E 80 s 70 PPIP 1 m F 2 60 a pn u 04 r E Q 40 a E PPIP 2 20 il 10 E 0 E T T T T 1 a 0 5 0 0 5 1 1 5 2 2 5 2 0 1 0 0 0 1 0 2 0 3 0 CV V Compensation oltage 2 90 a NPIP 2 70 4 a 2 2 60 o 5 50 f m a ia ners S 37 NPIP 1 i 20 1 5 10 4 T 5 0 E l l cr 1 5 1 0 5 0 0 5 1 1 5 1 I I I 3 e n E E AESA An SRS he 20 a an D CV V Compensation oltage Figure 49 On the left are examples of positive blue and negative red mode DF matrices recorded at the same time while a sample was introduced into the FAIMS detector The sample contained 5 chemical species which showed as two positive product ion peaks PPIP and three negative product ion peaks NPIP On the right the CV at the PIP s peak maximum is plotted against dispersion field to be stored as a spectral reference for subsequent samples 2010 OWLSTONE Ltd Proprietary and Confidential Page 47 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Positive mode llegative mode Figure 50 Comparison of two new DF plots with the reference from Figure 49 It can be seen that in both positive and negative modes there are differences between the reference product ion
55. t the electrodes whereas others will pass through to hit the detector To filter the lt e ions of different mobilities onto the detector n en E plate a compensation voltage CV is scanned CV 5V om g between the top and bottom electrode see gt _ A Figure 46 This process realigns the g trajectories of the ions to hit the detector and enables a CV spectrum to be produced _ oo quio The ion s mobility is thus expressed as a Figure 46 Schematic of the ion trajectories at compensation voltage at a set electric field different compensation voltages and the resultant Figure 47 shows an example CV spectrum FAIMS spectrum of a complex sample where a de convolution technique has been employed to characterize each of the compounds Changing the applied RF peak to peak voltage orig electric field has a proportional effect on the merged narrow se oe lt i ion s mobility If this is increased after each CV spectrum a dispersion field matrix is constructed Figure 48 shows two examples of how this is represented both are negative mode dispersion field DF sweeps of the same chemical The term DF is sometimes used instead of electric field It is expressed as a percentage of the maximum peak to peak voltage used on the RF waveform The plot on the left is a waterfall image where each individual CV scan is represented by as sg compensation voltage x axis ion current y axis p P i and electric
56. u bar Plugins DF Matrix Settings Status Lonestar Data Collection Tab selection Continuous Configuration Warn Alarm J On screen keyboard Normal 43 Gas Flow L min ZEA l Idle 0 00 Pressure barg 0 00 System control Figure 6 Navigation tool bar Menu bar This provides access to the offline analysis tools and exporting and loading of configuration files Tab selection The tabs in this area allow you to access the main functionality of the LONESTAR system software Left clicking on the required tab will change the display in the system information area below On Screen keyboard If a keyboard is not connected to the LONESTAR instrument click on the keyboard icon to display the on screen keyboard System control This area displays the volume of gas flowing through the LONESTAR and the system pressure The ability to switch between positive and negative mode views can also be seen here The start button initiates the run to gather data and the status box above provides and indication of each DF sweep s progress The system alarm status is also displayed here 2010 OWLSTONE Ltd Proprietary and Confidential Page 12 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 DF sweep data Before DF sweep data can be gathered the LONESTAR system has to be configured The config
57. ulations If this system is used in a manner not specified by OWLSTONE Ltd the protection provided by the system could be impaired Contacting OWLSTONE Visit the OWLSTONE website www OWLSTONEnanotech com for up to date contact details and service support For general inquires please email info OWLS TONEnanotech com UK Office US Office 127 Cambridge Science Park OWLSTONE Nanotech Inc Milton Road 400 Rella Boulevard Cambridge Suite 160 CB4 OGD Suffern NY 10901 Tel 44 0 1223 428 200 Tel 1 845 533 4225 Fax 44 0 1223 428 201 Fax 1 845 533 4232 2010 OWLSTONE Ltd Proprietary and Confidential Page 3 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Recycling and Disposal This Product has been designed and manufactured with high quality materials and components which can be recycled and reused X4 This product is required to comply with the European Union s Waste amp Electrical amp Electronic Equipment WEEE Directive 2002 96 EC so should j 2 not be disposed of in normal waste In some locations the radioactive source has additional disposal requirements please consult OWLSTONE Ltd for details of our recycling and disposal program for this product For users outside the European Union consult local authorities for correct disposal or contact OWLSTONE Lid Certificate of Conformity OWLSTONE Ltd perfor
58. uration that was last used will be stored by the LONESTAR system so all of the following steps will not be required each and every time a run is started Different configurations can also be saved and loaded into the software when required A number of key system parameters must be stable before data can be gathered Please refer to the application notes provided with your LONESTAR system about these parameters Begin configuring the LONESTAR system by clicking on the Settings tab The system information area will be updated with the Settings options screen as shown in Figure 7 Settings options screen DF Matrix Settings Instrument Set Up DF Matrix Length sec Min DF Matrix Interval sec OFF ON AUTO Pump Gas Flow pape 142 9 5 E AY o OO Field Intensity l a i CY Sweep Interval sec OFF B Gas Purge Linear Custom f i y 1 CON me Sensor Heater Start So Average Cy Sweeps J 0 se Con S Filter Heater End o ee 00 Experiment Number of Lines Care Eid Ba Analyhes gt Concentration ppb Operator wt Results Path i Figure 7 Settings options screen At this point we are only concerned with the DF Matrix settings area of the screen 2010 OWLSTONE Ltd Proprietary and Confidential Page 13 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 DF Matrix Settings DF Matrix Length sec Min D
59. uts a delay between the end of a DF Matrix and the beginning of the next DF Matrix 2010 OWLSTONE Ltd Proprietary and Confidential Page 15 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Min CV Sweep Interval sec Setting this value puts a delay between each individual CV sweep in the DF Matrix DF Matrix Length sec this will update automatically as the above settings are changed The time shown represents the total time taken to capture one DF Matrix to the configuration created Once the DF Matrix settings have been configured click on the DF Matrix tab in the navigation bar File Plugins Help Configuration Default DF Matrix Settings Status Continuous M po Ea SENNA nd 0 0 Gas Flow L min oD ale e eee Warn 9 Lonestar Data Collection Alarm 0 00 Pressure barg Prefix Logging To Positive Mode DF Matrix Negative Mode DF Matrix 100 0 100 0 90 0 90 0 80 0 80 0 70 0 70 0 60 0 60 0 c00 z 50 0 40 0 Era E 4o Dp 30 0 8 300 20 0 5l 20 0 ants e E 10 0 an meg 8 4 o 0 l l l I l l 0 1 7 eee 6 0 4 0 2 0 0 0 20 4 0 6 0 Compensation Yoltage Y Sener arr Usd UOT syeIsay S Juang UOT Heta Dispersion Field 5 full scale Dispersion Field full scale Positive Mode CY Sweep Negative Mode CY Sweep 1 I 1 1 I I Ion Current A U
60. y be an exception to this rule If a peak that does not move left or right is genuine then its height and width will change with increasing DF value This can be monitored using the offline software where the integrated ion current should show a general downward trend If however this result is constant it may indicate the RF generation stage has broken and needs repair 2010 OWLSTONE Ltd Proprietary and Confidential Page 28 of 48 The Owlstone logo OWLSTONE and LONESTAR are trademarks of Owlstone Nanotech Inc OWL LONESTAR SYSTEM USER MANUAL V2 0 AAN 12 07 10 Expansion Ports The LONESTAR system possesses several interface ports Front and rear USB For use with Memory Sticks Keyboards and Mice only GPIO General Purpose Input Output port System integration port allowing additional sensor inputs and outputs via industry standard I C interface GPIO pin out Pin No Name Notes 1 DIO 0 Digital Input Output 0 5V 2 DIO 1 Digital Input Output 0 5V 3 GND GND 4 5V Max draw 200mA 5 AO Analogue Output 0 5V 6 DIO 2 Digital Input Output 0 5V 7 DIO 3 Digital Input Output 0 5V 8 Al 1 Analogue Input 0 10V 9 Al 2 Analogue Input 0 10V normally not connected Contact OWLSTONE for further details on GPIO system integration RJ45 Network connector Networking is available on the LONESTAR system via standard Microsoft Windows protocols to enable file transfer and update 2010 OWLSTONE Ltd

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