Cell Separation - Volt Tecnologia
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1. Spin Collect cells Plasma G9 Highly purified cells left untouched Start CD45 Gate CD3 PerCP Cy5 5 CD3 PerCP Cy5 5 in iu n CD56 PE CD56 PE FIGURE 6 RosetteSep Human NK Cell Enrichment Cocktail Catalog 15025 Starting with whole peripheral blood the NK cell content gated on CD45 cells of the isolated fraction typically ranges from 80 98 In the example above the purities of the start and isolated fractions are 7 2 and 92 4 respectively Hassle Free PBMC Isolation sepMate is a specialized cell processing tube that facilitates the isolation of PBMCs by density gradient centrifugation in just 15 minutes SeoMate tubes contain an insert that provides a barrier between the density gradient medium and blood SepMate eliminates the need for careful layering of blood onto the density gradient medium and allows for fast and easy harvesting of the isolated mononuclear cells with a simple pour Ready Sep Go amp RosetteSep and SepMate Cell Isolation Directly from Whole Blood in as Little as 25 Minutes The RosetteSep and SepMate system is an innovative platform for rapid and consistent cell isolation from whole blood in as little as 25 minutes The system combines a unique immunodensity cell isolation reagent RosetteSep with a specialized cell processing tube SepMate to minimize variability between users and allow efficient high throughput sample processing2. CD2 Cells CD3 Cells CD4 Cells CDs Cells CD10 Cells CD14 Cells Pan CD25 Cells CD34 Cells hESC Derived CD34 Cells co N hESC IPSC SSEA 4 Cells hESC IPSC Tra 1 60 Cells CD56 Cells CD138 Cells Syndecan 1 CD271 Cells EpCAM Cells MUC1 Cells Glycophorin A Depletion PE Selection Use with PE conjugated antibodies hES amp hiPS cell 85 95 Cultures hES amp hiPS cell 96 99 cultures PBMC 86 98 18357 PBMC BM 85 95 18357 18659 18356 96 98 18359 18352 WB BM 2 4 log depletion 18352RF 18551 18551RF 18561 PE Selection Large Labels 5 x 10 cells 18561RF FITC Selection Use with FITC conjugated antibodies 18552RF T 18553 Biotin Selection 18553RF Use with biotinylated antibodies APC Selection 18451 Use with APC conjugated antibodies 18451RF 18099 18099RF Isolate any human cell type by positive 18309 selection 18309RF Do It Yourself Selection Use your own mouse IgG antibody Human Custom Selection Kit Kits for Whole Blood Samples TYPICAL PERFORMANCE DATA REAGENT CELL TYPE CD2 Cells CD3 Cells CD4 Cells CD4 CD25 Cells i 15861 low high B CD4 CD127 CD25 Cells 84 93 15861RF CD4 CD 127 49 CD25 Cells CD34 Cells High Purity Cord Blood CD34 Cells CD138 Cells Syndecan 1 CD8 Cells CD14 Cells CD15 Cells CD19 Cells CD20 Cells CD33 Cells
3. CD56 Cells Kits for HLA Analysis CELL TYPE CD2 Cells CD3 Cells CD3 CD19 Cells CD15 Cells CD19 Cells CD19 CD20 Cells CD33 Cells Myeloid Cells CD33 amp CD66b CD34 Cells CD56 Cells 18657HLA PBMC 56 98 18657 18687HLA 96 100 18687HLARF 18051HLA PBMC 95 100 18051HLARF 93 99 W PBMC W PBMC PBMC W W PBMC W PBMC TYPICAL PERFORMANCE DATA TISSUE PURITY 98 100 REAGENT KIT CATALOG 18081HLA 18081HLARF 18684HLA 18684HLARF 18681HLA 18681HLARF 18054 18054RF 18084 18084RF 18454HLA 18454HLARF 18184HLA 18184HLARF 18257 18257RF 18287HLA 18287HLARF 18683HLA 18683HLARF 18056 18056RF 18086 18086RF 18055 18055RF 18085HLA 18085HLARF CD66b Cells 18682 VE 18682RF 19961HLA Total Lymphocytes W 90 97 19961HLARF 19051HLA PBMC 95 99 19051HLARF gee 19951HLA eo 19951HLARF 19054HLA PBMC 95 99 19054HLARF Ee 19954HLA 82 100 19954HLARF 18683 Myeloid CD33 amp CD66b 94 99 18683RF 18684 Lymphoid CD3 amp CD19 98 100 18684RF im 18289 CD45 Depletion 2 4 log depletion 18289RF Each WB kit contains enough reagents to process 60 mL of whole blood except Catalog 15862 15861 15864 19961HLA 19951HLA and 19954HLA which can process 200 mL of whole blood Catalog 18088 18085 and 18085HLA which can process 30 mL of BC Catalog 18086
4. T Cells 90 95 19765 19765RF CD11b Cells 92 96 18770 18770RF 87 95 19853 19853RF 87 98 18780 18780RF CBs T Cells CDite Cells 84 95 19753 197 53RF Cultured BM 87 98 18758 18758 96 nes e ipd ad col 87 98 18768 18768RF Dissociation Medium 94 98 19854 19854RF B Cells CD19 Cells 97 99 18754 18754RF 91 96 19754 19754RF 98 CD25 Cells CD4 T cells 95 97 18761 NK Cells 75 87 19755 CD49b DX5 Cells 88 97 18755 18755RE Monocytes 19761 19761RF CD90 Thy 1 2 96 99 18751 18751RF CD117 cKIT Cells 88 95 18757 18757RF Pan Dendritic Cells 54 76 19763 19763RF BM 87 99 Dendritic Cells 19764 19764RF Fetal liver 74 97 80 90 SCA1 Cells 87 97 18756 18756RF Neutrophils 19762 19762RF 83 94 SCA1 Biotin 80 98 18856 18856RF Hematopoietic 76 92 19756 19756RF PE Selection Ho dd 18554 18554RF Progenitor Cells Epithelial Cells Epithelial lt 1 targeted cells 19758 PE Selection Large Labels 5 x 10 cells 18564 18564RF Mammary Mammary 1 stem cell per Use with FITC conjugated Mesenchymal Stem 50 99 19771 Biotin Selection Use with biotinylated antibodies 18556 18556RF Progenitor Cells APC Selection Jes wn 18452 18452RF Mouse Custom Isolate any mouse cell type by 19709 19709RF antibodies Enrichment Kit negative selection Mouse Custom Isolate any mouse cell type by Selection Kit positive selection Other Species Positive Selection Mouse Positive Selecti
5. This method isolates untouched and highly purified cells in a single step without columns or magnets minimizing the risk of activating or damaging cells Typical RosetteSep and SepMate System Protocol Incubate your blood sample with RosetteSep M Add density gradient medium to SepMate After a 10 minute centrifugation with the brake for 10 minutes at room temperature tube then rapidly pipet or pour the blood sample on simply pour highly purified target cells into directly over the insert a new tube Fast and Easy Isolate cells from whole blood in as little as 25 minutes with the HosetteSep and SepMate system Highly Viable and Functional Cells Isolated cells are functional unlabeled and flow cytometry compatible RosetteSep and SepMate Introduction www stemcell com SepMateVideo No Special Training or Equipment RosetteSep and SepMate can be used by anyone with minimal training No columns magnets or other special equipment are required Technical Bulletin A Novel System for High Throughput Cell Isolation Directly from Blood in 25 Minutes This technical bulletin presents data from Dr Ajay Jain s lab at the University of Maryland School of Medicine which used the RosetteSep M and SepMate system to reduce NK cell isolation time from four hours to a single hour for a 450 mL unit of blood To download this technical bulletin please visit www stemcell com CytotoxicityTB TECHNOLOGIES
6. which can process 75 mL of WB or 37 mL of BC Catalog 15086 which can process 120 mL of WB Catalog 18085 which can process up to 2 x 10 cells and Catalog 18289 which can proccess up to 100 mL of WB Each HLA PBMC kit contains enough reagents to process 10 cells except Catalog 18056 which can process up to 5 x 10 cells WB B WB B WB B B B BC B B Glycophorin A 18352 Please see page 15 for RosetteSep HLA cocktails 1 EasySep Reagent Kits include a bottle of EasySep M RBC Lysis Buffer 10X concentrate RoboSep Reagent Kits contain an EasySep Selection Kit with 1 2 boxes of RoboSep Tip Racks and RoboSep Buffer 2 Combined EasySep and RoboSep purity data when available Contains HetaSeop in place of EasySep M RBC Lysis Buffer 10X concentrate 4 PBMC peripheral blood mononuclear cells WB whole blood BC buffy coat RBC Lysis Buffer may be purchased separately 10X concentrate 100 mL Catalog 20120 Mouse Negative Selection Mouse Positive Selection TYPICAL TYPICAL PERFORMANCE REAGENT KIT PERFORMANCE REAGENT KIT CELL TYPE DATA CELL TYPE DATA TISSUE PURITY CATALOG TISSUE PURITY CATALOG 92 98 19851 19851RF CD4 Cells 94 99 18752 18752RF T Cells 95 99 19751 19751RF CD4 CD25 Cells 85 97 19782 89 96 19852 19852RF CD4 CD25 Large 85 97 19792 CD4 T Cells 89 19752 19752RF CD8a Cells 90 98 18753 18753RF Naive CD4
7. 059 190588 19081 a R082 2259 n SSR Hematopoietic 50 1 E Progenitor Cells with CD41 Depletion Use a custom 19309 19309RF Human Custom Enrichment Kit Each kit contains enough reagents to select desired cells from 10 total cells except for Catalog 18056 18167 and 18561 which can process up to 5 x 10 cells and Catalog 19232 19061 19062 18164 18096 18357 and 18659 which can process up to 2 x 10 cells Kits contain human Fc receptor blocker when necessary to prevent non specific binding antibody cocktail 1 RoboSep Reagent Kits contain an EasySep Selection Kit with RoboSep Buffer and 1 2 boxes of RoboSep Tip Racks 2 PBMC peripheral blood mononuclear cells PMNC polymorphonuclear cells BM bone marrow CB cord blood WB whole blood 3 Combined purity data of EasySep and RoboSep kits when available 4 Recommended for use with CLL and other abnormal samples 5 Pre enrichment kit optimized for maximum cell recovery subsequent cell sorting may be required 6 RosetteSep enriched Catalog 15062 Human Positive Selection TYPICAL PERFORMANCE CELL Mero Gois 5 48 Th1 Cells 85 95 Th17 Cells 85 94 CD4 CD25 Cells 90 98 CD25 Cells 90 98 V unn is orPBMC 85 99 18056 180556HF Frozen CB 84 99 18096 18096RF Fresh CB 81 98 Differentiated hES amp hiPS cell 18167 cultures 18165 18166 18055 18055RF
8. Cell Separation In As Little As 15 Minutes Table of Contents 3 4 10 11 12 Ready Sep Go Cell Separation in as Little as 15 Minutes EasySeo for Human Cells Immunomagnetic Cell Isolation in as Little as 25 Minutes EasySeo for Mouse Cells Immunomagnetic Cell Isolation in as Little as 15 Minutes RoboSep Fully Automated Immunomagnetic Cell Separation RoboSep Applications RosetteSep Cell Isolation Directly from Whole Blood with a Simple Spin sepMate Hassle Free PBMC Isolation RosetteSeo and SepMate Cell Isolation Directly from Whole Blood in as Little as 25 Minutes Research and Applications Equipment Product Listings Scientists Helping Scientists STEMCELL Technologies is a leader in the development of specialty cell culture media cell separation products and ancillary reagents for life science research Driven by science we deliver over 1500 products to more than 70 countries worldwide To learn more about how STEMCELL Technologies helps to make research work visit www stemcell com TECHN OLOGIES 5 e o Rea dy Sep Go FAST AND EASY Cells are ready in as little as 15 minutes with no columns Cell Se pa ration VERSATILE Isolate cells directly from virtually any sample source including whole blood and in as Little as 15 Minutes leukapheresis samples Isolate highly purified cells from a wide range of sample sources GENTLE Highly purif
9. EasySep Mouse T Cell Isolation Kit Catalog 19851 FIGURE 4 EasySep Mouse CD11c Positive Selection Kit II Starting with mouse splenocytes the T cell content of the isolated fraction Catalog 18780 typically ranges from 92 99 Starting with mouse splenocytes the CD11c cell content of the isolated fraction typically ranges from 87 98 Customized Cell Isolation of Virtually Any Cell Type From Any Species Can t find the cell isolation kit you need Our experienced scientists will work with you to create tailor made products or modify existing kits to meet your needs You can also use your own biotin PE APC or FITC conjugated antibody or any mouse antibody Please see page 14 for details or visit us at www stemcell com SstTEMCEL Fully Automated Immunomagnetic Cell Separation streamline your cell isolations using RoboSep the fully automated cell separator By performing all EasySep cell labeling and separation steps RoboSep M maintains the speed and simplicity of EasySep M while offering walk away automation This enables high throughput and versatile isolation of highly purified cells Minimize sample handling and eliminate cross contamination while isolating cells with just 5 minutes of hands on technologist time Press Select protocol Load sample EasySeo selection cocktail buffer and tips in carousel Fast and Efficient Isolated cells are immediately available for flow cyt
10. J Immunol 185 1927 2010 13 M Heinig et al Nature 467 460 2010 14 H Mkhikian et al Nat Commun 2 334 2011 15 A Doreau et al Nat Immunol 10 778 2009 16 L A Honigberg et al Proc Natl Acad Sci U S A 107 13075 2010 17 D Asslaber et al Blood 11 4191 2010 18 L Shao et al J Mol Diagn 12 670 2010 19 H Makishima et al Leuk Res 34 447 2010 SS d SOT ub E Copyright 2013 by STEMCELL Technologies Inc All rights reserved including graphics and images STEMCELL Technologies amp Design STEMCELL Shield Design Scientists Helping Scientists EasySep RoboSep RosetteSep SepMate RapidSpeheres and HetaSep are trademarks of STEMCELL Technologies Inc All other trademarks are the property of their respective holders Scientists Helping Scientists WWW STEMCELL COM TOLL FREE PHONE 1 800 667 0322 PHONE 1 604 877 0713 TECHSUPPORT STEMCELL COM INFOGSTEMCELL COM FOR FULL CONTACT DETAILS WORLDWIDE VISIT OUR WEBSITE STEMCELL TECHNOLOGIES INC S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS FOR RESEARCH USE ONLY NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES DOCUMENT 28960 VERSION 6 0 0 MARCH 2013
11. Research and Applications Fast and Easy Cell Separation Solutions The fast easy and column free cell isolation platforms EasySep RoboSep RosetteSep M and SepMate are gentle on cells and oreserve cell viability ensuring that isolated cells are suitable for downstream functional and biological studies Immunology and Infectious Disease Research With a full range of products for the rapid and efficient isolation of both human and non human immune cells STEMCELL Technologies cell isolation platforms have supported studies on e Pathogens including HIV influenza and other viruses bacteria and parasites e Autoimmune diseases including diabetes rheumatoid arthritis multipole sclerosis and systemic lupus erythematosus e Cancer Chimerism and HLA Testing STEMCELL Technologies provides highly optimized cell separation solutions that are suitable for e Chimerism analysis Flow Cytometry Crossmatch FCXM e Serology based assays Hematological Malignancies scientists worldwide use STEMCELL Technologies cell isolation platforms to purify malignant immune cells which can greatly improve the accuracy and reliability of research on e Multiple Myeloma e Chronic Lymphocytic Leukemia e Myelodysplastic Syndrome Assay Development STEMCELL Technologies cell isolation products have been used to isolate highly purified cells from a variety of sample sources and sizes for e Drug discovery and development e I
12. atalog 19054 Starting with peripheral blood mononuclear cells the CD19 cell content of the isolated fraction typically ranges from 95 9996 In the example above the purities of the start and isolated fractions are 11 8 and 99 4 respectively VIDEO EasySep Powerful Immunomagnetic Isolation of Virtually Any Cell Type www stemcell com EasySepVideo 5 EasySep for Mouse Cells Immunomagnetic Cell Isolation in as Little as 15 Minutes EasySep kits are available for the isolation of mouse cells in as little as 15 minutes from a variety of sample sources including bone marrow lymph nodes spleen and whole blood Negative selection kits including our new next generation EasySep kits use biotinylated antibodies to target unwanted cells while positive selection kits use an antibody complex to select cells of interest Typical Next Generation EasySep Mouse Cell Isolation Protocol 1 2 3 Add EasySep isolation cocktail Add EasySep Streptavidin Place tube in magnet for Pour off desired fraction into to single cell suspension RapidSpheres 2 5 minutes anew tube TEasySep P Incubate 2 5 minutes Incubate 10 minutes Cell suspension Times are typical for negative selection kits Times for each kit will vary depending on the exact isolation protocol Isolated Isolated a b a ie ial e i CD11c FITC CD11c FITC FIGURE 3
13. ds can be linked together Catalog 18130 5 amp System is Supplied With e RoboSep Carousel e Hydraulic Fluid Bottle e One Year Warranty e RoboSep Accessory Kit 4 The Big Easy EasySep Magnets 2 Boxes of RoboSep Filter Tip Racks 16 Filter Tip Racks in total RoboSep Buffer 250 mL RoboSep Service Rack USB Keyboard Mouse Memory Stick Magnet Shields Tip Head Polishing Compound User Manual RoboSep Timer Quick Start Sheet RoboSep amp Accessories PRODUCT NAME CATALOG 1 RoboSep Buffer and 1 2 boxes of RoboSep Filter Tip Racks are included with every purchase of a RoboSep Reagent Kit 2 10 mL of 10X RBC Lysis Buffer is included with every purchase of an EasySep or RoboSep Whole Blood Selection Kit see pages 12 13 Technical Specifications DIMENSIONS e Height with lid 56 cm 21 7 87 e Width 71 cm 27 7 8 e Depth 39 cm 15 3 8 e Weight 26 kg 57 Ib POWER REQUIREMENTS e 50 60 Hz AC 100 240V e Connections RJ 45 10 100 Ethernet port 2 USB ports CONDITIONS FOR OPERATION e Temperature 10 30 C 50 86 F RoboSep is not specified for use in a cold room 4 C 39 F e Humidity 20 85 non condensing Human Negative Selection TYPICAL PERFORMANCE eae CELL TYPE DATA 1905 IRF R082 CD4 CD127 T Cells t new HEN 190538 HE 9255 asa a HM 94h
14. ers Are Using RoboSep RoboSep is the cell isolation instrument of choice for leading laboratories worldwide because it fully automates cell isolation minimizes hands on time for technicians and enables high throughput sample processing These labs use RoboSep for a wide variety of applications including e Safe isolation of HIV infected T cells e Isolation of plasma cells to improve detection of cytogenetic abnormalities in multiple myeloma samples compared to column based cell isolation systems e Isolation of multiple cell types from a single undivided starting blood sample for chimerism analysis HIV Research Profiles STEMCELL Technologies products are used by HIV research groups worldwide where top scientists are conducting studies using robust physiologically relevant models performed with ex vivo primary cells The insights gained through this research facilitate progress towards more effective treatments and represent some of the most promising avenues towards an HIV vaccine To learn more about how leading researchers are using cell isolation for more physiologically relevant HIV research read our HIV Research Profiles at www stemcell com HIVResearchProfiles Capacity Positive Selection Processes up to 8 x 10 total cells 4 samples of up to 2 x 10 cells each Negative Selection Processes up to 4 x 10 total cells 4 samples of up to 1 x 10 cells each Negative and positive selections can be executed s
15. ied viable cells are immediately and sizes in as little as 15 minutes with the fast easy and column ready for functional and biological studies free cell separation platforms by STEMCELL Technologies TLasySepD Fast and Easy Immunomagnetic Cell Separation The EasySep system isolates cells quickly and easily without the use of columns in as little as 15 minutes With a simple pour isolated functional cells are immediately ready for downstream use Fully Automated Immunomagnetic Cell Separation RoboSep fully automates all cell labeling and separation steps of the EasySeo procedure minimizing sample handling and freeing up technician time efto TM amp hRosetteSep Unique Immunodensity Cell Separation RosetteSep isolates highly purified cells directly from human whole blood during density gradient centrifugation reducing your cell isolation workflow to a single step OSepMate Hassle Free PBMC Isolation SepMate enables consistent and hassle free peripheral blood mononuclear cell PBMC isolation in just 15 minutes minimizing variability between separations amp STEMCELL TECHNOLOGIES EasySep for Human Cells Immunomagnetic Cell Isolation in as Little as 25 Minutes EasySep combines the specificity of monoclonal antibodies with the simplicity of a column free magnetic system for effortless isolation of highly purified cells that are immediately ready for downstream applications Cells are cr
16. imultaneously Sample Volume From 250 uL to 8 5 mL per sample WEBSITE See RoboSep in Action www robosep com POSTER The Immune Response to HIV www stemcell com HIVPoster TECHNOLOGIES s RosetteSep Cell Isolation Directly from Whole Blood with a Simple Spin RosetteSep is a fast and easy immunodensity procedure for the isolation of untouched cells directly from whole blood By crosslinking unwanted cells to red blood cells RBCs present in the sample RosetteSep eliminates the need for a separate magnetic separation step because cells are purified during standard density gradient centrifugation This approach significantly reduces handling time and maximizes convenience Typical RosetteSep Protocol 1 2 Add RosetteSep Layer over density antibody cocktail gradient medium m EasySep Incubate 20 minutes Density gradient medium Isolated CD45 Gate CD3 PerCP Cy5 5 CD3 PerCP Cy5 5 a 4 7 4 7 FIGURE 5 RosetteSep Human CD4 T Cell Enrichment Cocktail Catalog 15022 Starting with whole peripheral blood the CD4 T cell content gated on CD45 cells of the isolated fraction typically ranges from 81 9726 In the example above the purities of the start and isolated fractions are 18 4 and 96 6 respectively SepMate Enriched cells Density gradient medium Red blood cells and unwanted cells rosetted 3
17. mmune cell based assay development e Vaccine development and testing TECHNOLOGIES Equipment wEasySep EasySep Magnet The EasySep Magnet is designed to hold one 5 mL polystyrene tube to isolate up to 2 5 x 10 cells or up to 5 x 10 cells when isolating rare cells e g CD34 per separation Catalog 18000 The Big Easy EasySep Magnet The Big Easy EasySep Magnet is designed to hold one 14 mL polystyrene tube to isolate up to 10 cells or up to 2 x 10 cells when isolating rare cells e g CD34 per separation Catalog 18001 Easy 50 EasySep Magnet The Easy 50 EasySep Magnet is designed to hold one 50 mL conical tube to isolate up to 4 x 10 cells by negative selection from whole blood leukapheresis products or mouse splenocytes or 2 x 10 cells from PBMC per separation Catalog 18002 EasyPlate EasySep Magnet The EasyPlate EasySep Magnet which is designed to hold a standard 96 well plate enables high throughput cell isolation from small sample sizes up to 2 x 10 cells per well Catalog 18102 EasySep Multistand Separate up to 4 samples at once using the FasySep Multistand The multistand is designed to hold up to 4 EasySep M Magnets or 4 The Big Easy EasySep Magnets Catalog 18010 EasySep EasyStand Separate up to 6 samples at once using the EasySep EasyStand Each EasyStand can hold a single EasySep Magnet and up to 6 EasyStan
18. ometry functional studies or other downstream analysis Simultaneous and Sequential Isolations oimultaneous cell isolation from up to four samples in as little as 25 minutes or sequential isolation of different cell types from the same sample Versatile and Safe Positive or negative selection from virtually any cell type from any species or source including whole blood with minimal sample handling Heturn in 25 to 60 minutes to collect your separated cells No Sample Contamination By using disposable tips in a column free system RoboSep prevents sample cross contamination Minimal Sample Handling RoboSep fully automates all sample processing steps reducing the potential risk of exposure to dangerous pathogens faced by researchers who handle biological samples Reduced Hands On Time In a busy lab technologist and scientist time is at a premium RoboSep can process up to 32 samples in an average working day with only 2 5 minutes of hands on time per isolation Using our old system it would take up to 30 minutes per sample because of the volume and we d have to be there all the time to load unload and clean the machine Now we can run four samples at once and have our enriched cells in an hour We re a busy lab and RoboSep really saves us a lot of time Kim Henderson Researcher CLINICAL LAB MIDWESTERN UNITED STATES TECHNOLOGIES 5 RoboSep Applications How Research
19. on Kits contain enough reagents to select desired cells from 2 x 10 total cells except Catalog 19782 18554 18555 REAGENT KIT 18556 and 18452 which can process up to 10 cells Catalog 19792 PRODUCT NAME DESCRIPTION CATAOG amp which can process up to 4 x 10 cells and Catalog 418564 which can process up to 5 x 10 cells Mouse Negative Selection Kits and Other PE Selection Kit d PE conjugated 18557 18557RF Species Positive Selection Kits contain enough reagents to enrich desired SUN cells from 10 total cells except Catalog 19763 and 19764 which can Use with FITC process up to 2 x 10 cells and Catalog 19771 which can process up FITC Selection Kit 18558 18558RF ch conjugated antibodies to 4 x 10 cells Kits contain mouse Fc receptor blocker when necessary to T prevent non specific binding Biotin Selection Kit S md 18559 18559RF BIOS 1 RoboSep Reagent Kits contain an EasySep Selection Kit with i RoboSep Buffer and 1 2 boxes of RoboSep Tip Racks APC Selection Kit al MM 18453 18453RF j pe Jug 2 bone marrow WB whole blood Do It Yourself Use your own mouse Selection Kit IgG antibody 18098 18098RF 3 ODE purity data of EasySep M and RoboSep kits when available Isolate any rat cell type SSSI MB lina 19609 4 Kits contain pre enrichment cocktail and two fluorescently labeled antibodies for subsequent cell sorting Isolate any n
20. on human primate cell type by 5 EasySep enriched Catalog 19772 positive selection Hhesus Custom Selection Kit RosetteSep Product Listing Human Negative Selection CATALOG TO LABEL 40 ML TO LABEL 200 ML ENRICHMENT COCKTAILS T Cells 15021 15061 15022 15062 CD4 T Cells CD8 T Cells CD4 CD25 Regulatory T Cells B Cells NK Cells Total Lymphocytes Monocytes Progenitor Cells from Cord Blood De Bulking Cord Blood for Freezing Progenitor Cells from Bone Marrow Mesenchymal Stem Cells from Bone Marrow Multiple Myeloma B and Plasma Cells from Bone Marrow Circulating Epithelial Tumor Cells Extensive Enrichment Circulating Epithelial Tumor Cells CD45 Depletion Any Cells Custom TO LABEL 40 ML TO LABEL 200 ML 0 075 CDS Cell Depletion Granulocyte Depletion CD66b Monocyte Depletion CD36 TO LABEL 250 ML TO LABEL 1000 ML 0 075 erma _ 07801 250 mL 07811 4 x 250 mL 07851 500 mL 07861 6 x 500 mL DEPLETION COCKTAILS CD3 Cell Depletion CD4 Cell Depletion HLA COCKTAILS T Cell Enrichment B Cell Enrichment Total Lymphocyte Enrichment Lymphoid Enrichment Myeloid Enrichment Granulocyte Depletion SUPPORT REAGENTS Lymphoprep RosetteSep DM L RosetteSep DM M 15705 100 mL 15726 100 mL 07906 100 mL HetaSep 1 Depletion cock
21. oss linked to EasySep M magnetic particles using antibody complexes and then easily separated from unwanted cells with EasySep magnet Typical EasySep Human Cell Isolation Protocol 1 2 3 magnetic particles Place tube in magnet for Collect cells accordingly as little as 2 5 minutes Add EasySep selection cocktail to single cell suspension Negative Selection Untouched desired cells are poured off in supernatant Incubate 5 minutes Incubate 10 minutes Positive Selection Desired cells remain in magnet EasySep magnetic particles do not interfere with downstream applications Cell suspension Times are typical for negative selection kits Times for each kit will vary depending on the exact isolation protocol Isolated Isolated CD3 PerCP Cy5 5 CD3 PerCP Cy5 5 CD3 FITC CD3 FITC 7 CD4 FITC CD4 FITC CD19 PE CD19 PE FIGURE 1 EasySep Human CD4 T Cell Enrichment Kit Catalog 19052 Starting with peripheral blood mononuclear cells the CD4 T cell content of the isolated fraction typically ranges from 92 97 In the example above the purities of the start and isolated fractions are 37 9 and 94 2 respectively Fast and Easy Isolate cells without columns High Purity Get purities of up to 99 Untouched Cells Use unlabeled cells immediately in functional studies FIGURE 2 EasySep Human B Cell Enrichment Kit C
22. tails can be added to a standard RosetteSep cocktail if not already present SepMate SepMate is a specialized cell processing tube that facilitates the isolation of PBMCs or specific cell types by density gradient centrifugation Catalog 15450 20 tubes 15460 100 tubes Antibodies Cell isolation and cell culture are essential steps for cutting edge cell based research Screening antibodies shouldn t be Don t waste valuable research time finding the appropriate antibody for your cell analysis ee STEMCELL Technologies now offers a line of high quality primary and secondary antibodies that are verified to work with our cell isolation and cell culture reagents in specific applications ensuring that your downstream cell analysis including phenotyping and purity assessments works consistently Visit www stemcell com Antibodies to learn more References A S ez Ciri n et al Nat Protoc 5 1033 2010 o Shetty et al Int J Hematol 95 274 2012 N Daguindau et al Clin Immunol 128 164 2008 T W Chun et al AIDS 24 2803 2010 S Sasaki et al J Clin Invest 121 3109 2011 B Oliviero et al J Hepatol 55 53 2011 L J Walker et al Blood 119 422 2012 H Jiafen et al J Immunol 177 8037 2006 S L Newman et al J Immunol 176 1806 2006 10 G Magri et al Blood 117 848 2011 11 J P Gigley et al Infect Immun 77 5380 2009 12 D E De Almeida et al
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