How to use the Peloris Dual Retort Rapid Tissue Processor
Contents
1. Ib Pacific Pathology Associates Inc Clinical Anatomic Pathology Histology RL Procedure histo17 01 How to use the Peloris Dual Retort Rapid Tissue Processor Final Approval May 2010 Effective May 2010 Next Review Date May 2015 List all stakeholder s and dates of approval Stakeholder Name s Shelly M Siegel HT Date 8 25 2010 Reviewed Revised _ Stakeholder Name s Mark Magilner MD Date 8 2710 Reviewed xX Revised L_ Stakeholder Name s Shelly Siegel Date 5 19 14 Reviewed xX Revised _ Stakeholder Name s Shelly Siegel Date 6 15 12 Reviewed X Revised Stakeholder Name s Shelly Siegel HT Date 5 30 13 Reviewed xX Revised _ Describe briefly the most recent revision made to this policy procedure or protocol amp why New Procedure Purpose Policy Statement This procedure addresses on how to start change reagents and do daily weekly and monthly maintenance on the Peloris Definitions e na POLICY CONTENT The Peloris dual retort rapid tissue processor preserves tissue specimens by fixing in 10 neutral buffered formalin dehydrating in alcohols clearing in xylene then infiltrates with wax This allows tissue to be sectioned stained and ready to be diagnosed by a pathologist STEPS KEY POINTS TO START THE PELORIS Step 1 Load the protocol e Press the SELECT or PROTOCOL button Choose the protocol SMALL MEDIUM or LARGE Press LOAD Pick the retort you are lo2. ading A or B Step 2 Start the Run e Press RUN e Enter the of CASSETTES Press OK e Prompts you to load Press DONE Step 3 Scheduling e Select the endtime to ASAP should read required endtime ASAP The ASAP button will be highlighted in Orange e Press the GREEN START button How to use the Peloris Dual Retort Rapid Tissue Processor Page 1 of 4 REAGENT WAX REPLACEMENT Replace reagent wax when chamber appears striped on the status screen or when warned at protocol start Concentration threshold exceeded for last step Document all reagent wax changes on the equipment maintenance form REAGENT Step 1 Pull reagent bottle from peloris Step 2 Empty according to chemical disposal waste management either down the drain or in a labeled waste container to recycle or drum waste SINK 7 ies 1 2 10 Filtered Formalin DISPOSAL SINK A o o 3 10 55 100 Alcohol DISPOSAL 11 14 Xylene Pure Xylene 15 Cleaning Alcohol 16 Cleaning Xylene Recycled Xylene Step 3 Push the bottle back into the same station e State FULL e Press RESET Step 4 Document Maintenance e Initial Peloris maintenance log Step 1 Connect wax waste line hose e Pull metal tray found under peloris as a secondary container in case of spill e Rest hose into funnel in a 1 gallon container You will need 2 empty 1 gallon containers to empty wax station Step 2 Open the REAGENT tab then press REMOTE FILL DRAIN Sele
3. ct WAX WASTE and the wax chamber to drain then click DRAIN TO WASTE Monitor drain progress wait until notified of completion Go to Step 4 REPEAT step 2 to purge the remaining wax from the line to prevent wax buildup on exterior of machine You will hear the pump purging air which is normal Go to step 3 Step 3 Add new wax e Clean the wax bath using gauze e Add molten paraffin to MAX line Do not over fill Step 4 Update Station State e Open the REAGENT tab then find STATIONS e Press the WAX CHAMBER e Press the DRY state on the wax chamber that was emptied e Press FULL Step 5 Disconnect wax waste line hose e Cap both wax waste gallon containers and place in biohazard box e Replace funnel used for wax disposal under fume hood e Replace metal tray under Peloris Change absorbant pads as needed Step 6 Document Maintenance e Initial Peloris maintenance log MAINTENANCE How to use the Peloris Dual Retort Rapid Tissue Processor Page 2 of 4 Document on form Equipment Maintenance for Peloris H Lab Anatomic Pathology Histology Forms PELORIS doc DAILY e Check reagent wax levels Make sure reagent wax is not over or under the MAX line e Clean the top surface of the retort lids and handle WEEKLY e Check condensation bottle empty if needed e Clean gasket seal on retort with 70 100 alcohol Make sure it is free of paraffin e Clean sensors with 70 100 alcohol e Clean touch screen QUARTERLY e Change carbon f
4. gulatory Requirement Yes _ No _ If yes insert requirement information here Review History No Changes Shelly Siegel Date 5 3 11 Reviewed X Revised _ How to use the Peloris Dual Retort Rapid Tissue Processor Page 3 of 4 Revision History Note changes in area under header Computer Search Words Policy Procedure or Protocol Cross Reference Information n a How to use the Peloris Dual Retort Rapid Tissue Processor Page 4 of 4
5. ilter JAN APR JUL OCT Supervisor needs to sign in and update carbon filter age Go to Controls Instrument Settings Carbon Filter age Reset e Clean reagent bottle Use lab detergent Lysol to clean not bleach List of Daily Weekly and Maintenance Checks can be found on the in service sheet H Lab Anatomic Pathology Histology Peloris list of in service doc Equipment Supplies If Applicable Alcohol graded reagent Xylene Recycled Xylene for cleaning only PPE gloves goggles apron or face mask Funnel Paraffin Cassette basket Gauze paper towel 10 NB formalin Can use filtered if available Form Name amp Number or Attachment Name If Applicable Histo17 Chemical Disposal of waste H Lab Anatomic Pathology Histology Processing Chemical Disposal for Tissue Processors doc Equipment Maintenance sheet H Lab Anatomic Pathology Histology Forms PELORIS doc List of in service H Lab Anatomic Pathology Histology Peloris list of in service doc Total Fixation on the Processor H Lab Anatomic Pathology Histology Processing Total Fixation Peloris Protocols H Lab Anatomic Pathology Histology Peloris Schedule doc Author Position Lead Histologist Review Revision Authority Position Not Individual Name Lead Histologist Expert Consultant Position s Not Individual Name s n a References Required for Clinical Documents Peloris Rapid Tissue Processor user manual Peloris alarm and error code manual Is there a Re
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