128 Channel Recording System
Contents
1. Headstage e Default Total System voltage gain of 800 other gain offerings available e Rechargeable battery with 3 5 4 2 hours of battery life AT gt Ss 3 ee wm Wem TEE e Bandpass filtering per channel at 8 Hz to 7 kHz typical Neuro4jWare e 50 kHz per channel sampling rate e Operates in unlicensed radio band below FCC limits e Covered or dipped headstage options Receiver with Dual Antennae Version 3 0 3 3 2014 W128 System User s Manual 4 Mists System Configuration The receiver antennas must be clipped to a surface within 4 meters from the animal at all times to achieve optimal signal quality They should be positioned above the animal s cage Be careful not to obstruct the line of site path between the animal and the receiver antenna with any material except for glass or plastic See the section entitled System Setup and Testing for more details on proper system assembly and positioning TBS NeuroWare Software Analog Receiver with ADC Board Wireless Headstage USB Cable Standard TBSI Wireless Recording Setup System Block Diagram The wireless neural headstage system consists of a wireless transmitter headstage an RF receiver and baseband demodulation subsystems as shown below Headstage Transmitter Time Division Multiplexer RF Modulator Battery and voltage regulator RF Receiver Components2. Version 3 0 2 3 2014 W128 System User s Manual y IBS 128 CHANNEL WIRELESS NEURAL HEADSTAGE SYSTEM TESI NeuroWare Sottware Analog Receiver with ADC Board Wireless Headstage an d ege USB Cable tiil a lF PI Introduction The Triangle BioSystems International TBSI W128 wireless recording headstage system allows researchers to continuously and simultaneously monitor up to 127 single ended biopotentials as single units EEG and ECOG Differential signals such as an EMG recording can easily be accommodated in software No longer do experiments have to be constrained by tethering a test subject No longer do experiments have to be constrained by tethering a test subject The complete system is comprised of a wireless headstage transmitter with integrated battery RF signal receiver baseband demodulator power supply and all required cables With an effective range of 4 meters this system provides a wireless connection between the implanted electrodes and the data recording system TBSIs custom ASIC technology and proprietary radio design provide high data rates 50 kHz channel in a wireless headstage that is both small and light weight 10 0 g This design also incorporates preamplifier circuitry to create an extremely compact and powerful transmitter Features e Wireless operations across 4 meters Z e 127 single ended recording channels e Headstage transmitter weight 10 0 grams
3. System User s Manual A TBSI LED Headstage Options Red blue or green permanently affixed LEDs or socket mounted LEDs are available for the W128 headstage The LEDs are placed facing upward on top of the headstage and are suitable for use with most video tracking software applications The LEDs will turn on when the headstage is turned on Installation of LEDs on the headstage may reduce battery life by as much as 15 Headstage Removal Tool This tool is used to remove a headstage from the rat or mouse s head quickly and efficiently its part number is 300 0006 10 Headstage Electrode Connectors Alignment Tool 2xA9409 Female 36 pin 4 post 25mil Omnetics Connector Part Number 010 0059 12 Wireless Power Headstage Option This unique wireless power system is designed to keep a wireless headstage internal battery perpetually charged thereby enabling indefinitely and uninterrupted device operation There are 4 major components including the power amplifier transmitter transmitter coil around the animal s cage power supply and headstage pickup coil with harvester Since the transmitter coil is carefully tuned and sized for the animal cage dimensions it is important to note that the wireless power transmit coil must be placed carefully on a bench top or cage rack without nearby metal and at least 15 inches from other TBSI wireless powering systems Electrodes Many electrode manufactures offer electrode
4. 5 Ch82 Ch83 Ch112 Ch111 Ch16 Ch15 Ch46 Ch47 Ch80 Ch81 Ch110 Ch109 Ch18 Ch17 Ch48 Ch49 Ch78 Ch79 Ch108 Ch107 Ch20 Ch19 Ch50 Ch51 Ch76 Ch77 Ch106 Ch105 Ch22 Ch21 Ch52 Ch53 Ch74 Ch75 Ch104 Ch103 Ch24 Ch23 Ch54 Ch55 Ch72 Ch73 Ch102 Ch101 Ch26 Ch25 Ch56 Ch57 Ch70 Ch71 Ch100 Ch99 Ch28 Ch27 Ch58 Ch59 Ch68 Ch69 Ch98 Ch97 Ch20 Ch29 Ch60 Ch61 Ch66 Ch67 Ch96 ACGnd Ch32 Ch31 Ch62 Ch63 Ch64 Ch65 ACGnd ACGnd ACGnd ACGnd ACGnd ACGnd ACGnd ACGnd Guide Post RAJ Guide Post Guide Post L A Guide Post Guide Post Guide Post Guide Post Guide Post Version 3 0 7 3 2014 W128 System WOH Manual A TBSI RF Receiver Specifications e Two of the Three available transmission frequencies o F1 3 05 GHz center transmit frequency with 100 MHz FM bandwidth o F2 3 375 GHz center transmit frequency with 100 MHz FM bandwidth o F3 2 725 GHz center transmit frequency with 100 MHz FM bandwidth e 4 0 M maximum range between headstage transmitter and receiver antennae e Front end gain 60 dB e Intermediate gain 10 20 dB e Input referred noise typical 4 uV RMS e Input voltage range 0 5 V e Analog channel bandwidth 20 kHz e DC offset lt 100 uVdc e Phase delay typical 30 usec at 10 kHz e Signal lock indicator LED on front panel RF Receiver Mechanical Overview a kel enen t lt vne Neuro Ware 1 On Off Switch 2 Signal Lock LEDs Power On LED 3 Antenna SMA Connectors 4 Analog Output Connectors DB37 5 GN
5. D Connector Option 6 Receiver Power Supply Connector DC6V 8A Version 3 0 8 3 2014 W128 System User s Manual RF Receiver Signal Interface Options The W32 RF Receiver Base Station is available in many different system configurations to accommodate a wide range of data acquisition system interface requirements The RF receiver signal is demodulated by a custom digital signal processing system that incorporates a high speed analog to digital converter for demodulating the receiver signal The demodulated signal is then conditioned via the DSP and converted to an analog signal The analog signal can be directly output to one or two DB 37 connectors input to an integrated Analog to Digital Converter ADC or both Receiver Signals Analog Output Pal EES VO J i Version 3 0 9 3 2014 W128 System User s Manual Receiver Signals Digital Interface TBSI offers an internal DAQ board option and NeuroWare software for the 5ch 16ch 32ch and 64ch receivers which can be used for signal data acquisition and analysis Digital output and event input information is sent to the PC via a USB connection The total system gain for the digital receiver is 800 The integrated data acquisition card can also be read using DAQ toolkits from MatLab and Labview Stet mg Neurol Ware Eoen Hait Chenrel Setirg Ferdel Charme Record
6. Duta Spon Caplan Settings Filer Feiegsece Graph Settings Auto Trigger Sellia ar ire Trigger Tika Worth e i eu Hm Lpste ke arg be TH Chart Sage erte Signal Dos See Display Wide Window o And eegene x eege ee GC P Sek Wem fe Seepeches fe 100 S 6 Threshey kt Apply Conert Threshokd Io E Charis T Version 3 0 10 3 2014 W128 System User s Manual System Setup and Testin The steps below should be followed when setting up your W32 system to optimize its performance 1 Charging the Headstage Lithium Ion Battery The wireless headstage includes an integrated rechargeable battery which should be recharged when not in use Recharging is accomplished by the following procedure Charge Plug a Turn off the headstage transmitter using the magnetic wand blue light will turn off as indication b Connect the battery charging adapter to a wall socket LED Charge Indicator Headstage with c The white charging plug is keyed and ene mates with the white socket on the side of the headstage Connect the charging cable to the headstage making sure that the connection is sound d The indicator light on the charging unit is red while charging and green when the connected battery is halfway recharged A dead battery will become fully charged within approximately one and a half hours after the light has turned green 2 Correctly position the receiver
7. Pre amplifiers High BW Amplifier Electrode Inputs Pass Filters Transmit Antenna RF Filter amp 60dB Gain RF FM Demodulator P RF link Baseband Demodulator Components ge To Neural d E SE D E a E a EE DAQ Output Leen _ Analog e PlLandCik system S Bandpass Filter De multiplexer TE E CR EES q Bieta A Recovery Zen Ge KE a NOTE These products are not intended for human use Version 3 0 4 3 2014 W128 System User s Manual 7 IBS Wireless System Parts List Nowra Ware Wireless System Parts List 300 0002 10 Receiver Antenna Extension Cable and Mounting Camp 5ft o 1000000100 Record or Stim Wireless Headstage Battery Charger 47V 0021 8 300 0000 00 Magnetic Wand Headstage On Of 12In 0 O O a mme 200 0021 10 W32 Signal Input Test Cable All Channels Combined Single GND 202 0001 10 Male DB 37 Connector and housing 200 0011 00 4 foot earth GND cable for receiver banana jack Not Pictured Version 3 0 5 3 2014 W128 System User s Manual Headstage Transmitter Specifications PARAMETER MAX units NOTES S A Standard Version B High Power Version Current Draw 48 Battery E 3 A O oa 5 KG et dal VG ad CH L i Input voltage range mVp p N LO O CO CH O Gain Selection Bandwidth Input impedance 3 dB input signal level BW m
8. Triangle BioS Inter ti TBSI W128 System Manual Version 3 0 Triangle BioSystems International 2224 Page Rd Suite 108 Durham NC 27703 Phone 919 361 2663 e Fax 919 544 3061 www trianglebiosystems com W128 System User s Manual Contents 128 CHANNEL WIRELESS NEURAL HEADSTAGE SYSTEM 3 Idee Te den DEE 3 Ree 3 VS Ce COI MS O EE 4 Systemi Block Did SPAM EE 4 Wireless System Parts LIST cesccccccdscovsesieesdnsnastaincsensbavesdueapabedessasietenseudesabedcasogusivateuensedentausdiedebiwesndesandaussuauengeneds kariaia oiii 5 Headstage Transmitter Specflcattons ettres tettest tetese r tt rst tt rettete ser trors seseseneeeeesueneesets 6 Headstage Mechanical Overvlew tetter r ttres t111 est tt esetet ert Ert reeter r tetro rs etrese terereeerereeee 7 Wireless Headstage PINOUT EEN 7 RF Receiver SITS CITLCa HIOINS seniman S EA EEEE EEA EE E EREE 8 RF Receive Mechanical OVEMVIEW E 8 RF RECEIVE Sienal interlace EE 9 Receiver signas Anao e E 9 Receiver siena KT SCH ae 10 SR EE a WK du 11 be rinall and Extemal Ee dn EE 14 Mouse and Rat Saddle Harness siicrisirncnancurriinarnrsrei ni tana ken EKANIN TAE A ENAA A Ea OKRASA Eaei a T ERT SA a aE EA S an ea 15 Peddi bt EEAO ON 0 1173 EE 15 LED Regdstace Re d EE 16 FR AGS Ge Se un Vall TOO DEE 16 Headstage Electrode Connectors Alignment Tool 16 Wireless Power Headstage Optton 16 Segel 16 Gainand Phas RESPONSE E 17 TOUPIES NOONE E 17 leit gel Weg 18 COE EE 18
9. adstage These accessories replace channels typically used for neural recording on the headstage Installation of any of these features increases the headstage length by 0 4 inches its height by 0 2 inches and its weight by 0 4 grams The features can be installed individually or together in any combination See the TBSI Biosensors Brochure for more information Due to the fact that sensor options require 1 or 3 data channels each these may not be practical for use with the W16 headstage consider a W16 or higher headstage Accelerometer This add on will monitor x y and z acceleration vectors and output the information via three analog signal channels The animal head position and velocity can later be calculated with software such as Matlab Ultrasonic Microphone This single ultrasonic microphone enables recording of audio frequencies between 1 kHz and 25 kHz W5 and W32 Headstages or 1 40 kHz W16 and W64 Headstages and uses one recording channel Temperature Sensor A Thermistor based temperature sensor option is available Many sensor sizes are available including very small ones that can be implanted within or near the brain The sensor must be attached to a mating connector and externalized to the head of the animal much like traditional recording electrodes Sensors are customized to your particular research needs i Accelerometer Ultrasonic Microphone R e Thermistor Connector Version 3 0 15 3 2014 W128
10. arrays compatible with TBSI wireless headstages Cambridge Neurotech offers an extensive line of silicone probes suitable for chronic signal recordings from our wireless headstages Compatible electrode arrays are also available from Microprobes Inc NeuroNexus and others electrode providers Version 3 0 16 3 2014 W128 System User s Manual Gain and Phase Response Please refer to the section of this document entitled Headstage Transmitter Specifications for numerical data on bandwidth and input referred noise GR 17 5 iw L t l i Leg Hz TEk Troubleshooting Problem No neural signals are visible on any of the analog outputs at the DB37 connector Suggestion Verify the AC power connection is in place and the green Power LED is illuminated on the front of the RF receiver box Also verify that the yellow Signal Lock light is illuminated which confirms that the receiver is receiving the signal transmitted from the headstage Problem Visible neural signal is missing information Suggestion Keep the animal within the 4 meter range of the receiver If you exceed this range the radio signal from the headstage will not be strong enough to maintain reliable signal monitoring of the animal Also be sure to keep the area under the RF receiver unit s antennas free from metallic objects which will reduce signal range and introduce noise Problem Not all channels are visible on the neural signal Sugg
11. configured with a 60 mAh internal battery The W32 headstage can also be built with an internal 75 mAh internal battery for increased recording capacity Alternatively the headstage can be configured for use with an external battery Several different external batteries are available and are listed in the table below For rodent applications we offer both a mouse and a rat saddle harness with Velcro pad See the section entitled Mouse and Rat Saddle Harness below Removing the internal battery will reduce the weight and size of the headstage by an amount comparable to the battery specifications as listed in the following table TBSI W Series External Batteries Weight 6B Se z me D Kb Weight 538 Am 538g Hp 1 Hours of Operation STD Standard transmission power headstage HPT High power transmission headstage International AC Power Adapters are External Battery and Headstage included each systems A complete set Connector of adapters can also be purchased separately Part AC5 100 0001 04 External Battery Charger 4 7 V 2 prong male connector Version 3 0 14 3 2014 W128 System User s Manual Mouse and Rat Saddle Harness Saddle harnesses can be used for external battery options Mouse Saddle Harness Rat Saddle Harness Rat Saddle Harness with Mounted 004 0001 10 004 0001 20 500 mAh External Battery Headstage Accessory Options Three biosensor options are available as add ons for any wireless he
12. en 810 a Factory selectable total system gain Oeo o At1kkz O Wms A A 7000 H Q uVrms for 1 Hz 7 kHz frequency 35 uVp p H At headstage max 30 kHz sampling rate channel at ADC Input referred noise h h Z Sampling Channel Z k Edge to Edge including connectors Edge to Edge 14 2 mm Edge to Edge wein 100 With connector and dipped package _ Inputconnector BP Omnetics 025 Radio Specs F1 F2 F3 Center frequency 3 05 3 375 With 100 MHz bandwidth 34 3 28 7 Length Width Height OD O gt DI e N OH OD O Go Un 00 Own FCC Sec 15 109B a Transmit antenna 3 05 3 375 2 725 GHz Tuned chip antenna with circular diversity __Transmitrange 40 40 40 mM __With connector and dipped package Version 3 0 6 3 2014 W128 System WOH Manual A TBSI Headstage Mechanical Overview Top View Bottom View 28 7 mm HN i Complete headstage weight is 5 0 grams Wireless Headstage Pinout Guide Post K K Guide Post Guide Post EU Guide Post Guide Post Guide Post Guide Post Guide Post ACGnd ACGnd ACGnd ACGnd ACGnd ACGnd ACGnd ACGnd Ch126 Ch125 Ch2 Ch1 Ch33 ACGnd Ch94 Ch95 Ch124 Ch123 Ch4 Ch3 Ch34 Ch35 Ch92 Ch93 Ch122 Chi2Zt Ch6 Ch5 Ch36 Ch37 Ch90 Ch91 Ch120 Ch119 Ch8 Ch7 Ch38 Ch39 Ch88 Ch89 Ch118 Ch117 Ch10 Ch9 Ch40 Ch41 Ch86 Ch87 Ch116 Ch115 Ch12 Chi Ch42 Ch43 Ch84 Ch85 Ch114 Ch113 Ch14 Ch13 Ch44 Ch4
13. estion Make sure the headstage connection to the animal is secure Version 3 0 17 3 2014 W128 System User s Manual Application Notes 1 The wireless headstage is a low power device it is critical that the RF receiver be carefully located for the system to operate DO NOT place the receiver on the outside of a metal wire cage Try to minimize the distance between the receiver and the wireless headstage Please refer to the section in this document entitled System Setup and Testing for orientation suggestions 2 If you do not intend to record from all of the available signal channels you must ground any unused channels at the electrode connector or EIB interface Failure to do so can create artifact noise from the floating channels on the other signal channels 3 Headstage Rubber Ban Dental Acrylate Metal S Be Hooks P CH Animal Skull Contact Us Triangle BioSystems International 2224 Page Rd Suite 108 Durham NC 27703 Phone 919 361 2663 e Fax 919 544 3061 www trianglebiosystems com Need more help You can reach TBSI customer support by phone at the number listed above M F Eastern US Time or email us at support trianglebiosystems com If you need replacement parts or accessories for your system or to learn the latest about available TBSI products visit our website call or email us at sales trianglebiosystems com Version 3 0 18 3 2014
14. f your receiver has an integrated DAQ plug the USB cord into the receiver and PC and follow the instructions provided in the Neuroware Manual or your data acquisition software for signal acquisition and viewing Plug and screw connector into back of receiver box tee 4 mete Wires to recording ADC System Sas a Gah Ee cd 5 Turn on Receiver Flip the on off button of the receiver to control power to the receiver 6 Signal Lock LED and measure analog output signals Once the receiver and headstage see 7 below have both been switched on the Signal Lock LED on the receiver front panel should light up After confirming the signal lock integrity you can view the analog signals output from the DB37 connector on the back of the receiver with an oscilloscope Please note the default system gain is 800 therefore you can expect the analog output values to be about 800 Vp p of the signal from the function generator Version 3 0 12 3 2014 W128 System User s Manual 7 Test headstage signal transmission with the W128 Signal Input Test Cable a Line up the two guide pins on the headstage with the guide pin holes on the test cable Make sure that the labels on the connectors face the same direction b W128 Signal Input Test Cable 200 0021 10 supplied with receiver c Signal leads connect to positive side of function generator d GND Leads connected to signal Ground side of function generator Input a 1
15. kHz sine wave signal at maximum 4 mVp p amplitude The function generator signal output voltage cannot exceed A mVp p otherwise the headstage input voltage range will saturate e Once the test cable has been attached use the magnetic wand to turn on the headstage Briefly hold the black tip of the wand next to the top of the headstage until you see the blue power on LED The headstage will transmit data continuously while this LED is on 8 Inductive Power Headstage and Charging TOP VIEW 1 Red battery is enabled O Green battery is charging Blue transmitter is enabled If the wireless power charging accessory is used please follow the steps below for charging the headstage a Plugin the Inductive charger power supply Turn the transmitter on using the power switch on the face of the Inductive power base station b Turn on the headstage charge coil using the RED end of the magnet wand On will be indicated by a RED LED c With the RED LED lit place the headstage in the Inductive transmit field A GREEN LED will indicate that the battery is being charged Charge the headstage for 2 hours to fully charge the headstage battery d Use the BLUE end of the magnet wand to turn on the TBSI headstage for recordings The headstage radio ON is indicated by a BLUE LED Version 3 0 13 3 2014 W128 System WOH Manual A j iste Internal and External Batteries The TBSI W16 recording headstage is typically
16. near the animal s cage It is critical for transmission performance to correctly position the receiver next to or inside the animal s cage First screw the antennas onto both antenna clamps gold input SMA connectors located on the side of each clamp Attach the SMA connector of the antenna cable to the mating SMA connector on the receiver box For best results position the antennae at 45 degree angles with respect to the cage area and aim the them towards center of the cage Version 3 0 11 3 2014 W128 System User s Manual 3 Connect power supply to receiver Plug the DC end of the power supply cable into the back of receiver and the other end into an AC outlet The recording system is powered by an AC line adapter transformer regulator This power unit connects to a 100 240 VAC at 47 63 Hz and is rated at 6 VDC at 2 5 A power source and is UL approved Use a suitable International adapter to mate the standard US plug to your AC outlet Power Supply 120 240 4 Connect signal cable to receiver analog output DB37 connector If your system was configured for analog system output connect a suitable analog output cable to the DB37 receiver connector Five signal wires and a ground wire can be added to the DB37 mating connector to check for signal output The lengths of the wires are not critical The analog output channel positions are described in the section entitled Receiver Signal Interface Analog Output I
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