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MagCore® Viral Nucleic Acid Extraction Kit

1. a Se gt SSS s s Sl Ssl g g S ela 8 es S S 3 SL a a A a G S S a S S S S S S o d A 2 amp g ET 38 38 8 g Ps Pis i pv l3 S R Sm A A S S S S 3 S zl en 3 o 3 o As ST IEIET 9 sigs ER SB N S S S e S m E im E B 8 82 8 S Oo Gy s e gt 5 o ad Hi Sy oS E MO moz Well Elution Tube Tip Tip Holder Tip Tip Holder Important Notes 1 The surface area of the FFPE tissue sample could be measured as following examples Medium Small gt 1 2cm 2 Sample amount of preparation can be 1 5 scrolls each with a thickness up to 5um One FFPE scroll could be enough to analyze if the surface area is over 200 mn Surface area mm Sample scroll 200 1 1 100 200 1 2 50 100 23 50 3 5 do not over Sula Oil s capacity 20mg Overload the sample or paraffin will clog the tip and decrease the yield 3 If the tissue sample is over 300 mm we recommend cutting it into 4 sections as following examples 4 Ifyou haveno information about the sample we recommend starting with no more than 1 scroll and cutting it into 4 sections per preparation 5 Sula Oil is a deparaffin buffer The capacity of the Sula Oil 500 ul is about 20 mg paraffin per preparation 6 In MagCore 405 program two different lyse time are provided 2hr and 16hr Recommend 1 B
2. and you might lose your samples by machine interruption EN i Processing i E yo Input Cartridge Code to run protocol Boc m JB d Heater B68 C 1544 F HeaterA68 C 1544 F A i Cartridge Code is shown on your Reagent Cartridge and the PROCEDURE COMPLETED sil Heating i coverofuser manual Crary conrunue oo Select srl iin iind mm s Above CODE is for demonstration purpose please refer pne a a Pe re See to the kit you purchase for proper program CODE i E iecur v While program finished a beep sound can be heard and Green HAE Indicate LED light went out eaaees 4 5 6 n i ae EE iu 8 9 Well Position of T Rack Display selected parameter items Elo JE Well Display current operation status Confirm your input code again and press Enter to next page for el 1 Please reconfirm the number you selected and select all sample volume selection nee parameters Heater status e 7 f M Well 4 W NextStep oO Emergency Stop and Cancel keys Please i OME Ss referto the content on page 9 VIRAL NA OOO COO EIA D o ere t 9 i f a A n M a W m oaa e S 3 A COO aq D Y You may watch the procedure status and remained time ofthe HESS PREV PASE E e OI e ar i current operation on the control panel The s
3. Cartridge Contents L A S a Se n gt g gt e SS gt gt T T JO JO m V r B B m y BS S3 g es gs S S D D a ua A a o A 4 A Q S S A A gt 7 a S eo S 3 35 gt Sy N 6 c So Sm 9 9 E 5 to ES cm em a a 9g e e e eo D oo oo 2s T T Zh Zh A S 3 c 35 c 2 m m S E i 3 3 3 n S N Q N 82 S8 s B Ele le sla e 5 EAR AEA Description MagCore Viral Nucleic Acid Large Volume Extraction Kit 1 2ml is designed for purification of DNA and RNA from 1 2 ml serum plasma cell free body fluids by MagCore auto extraction instrument With all the kit components of plastic consumables are DNase RNase Free pretreated and individual processing track for each loaded samples this system eliminates all possible cross contamination between samples Built in protocol with flexibility in sample source volumes both DNA and RNA virus can be extracted using the same kit in a fast and economical way Applications Using magnetic particle technology to purify viral nucleic acid from serum plasma or cell free body fluids The purified viral nucleic acid is suitable for highly sensitive and quantitative PCR MagCore Viral Nucleic Acid Large Volume Extraction Kit has been proven with HBV HCV HIV and influenza viruses for downstream applications PEE www rbcbioscience com MagCore Running Time 73 min
4. vess 7 Cartridge Code 102 CatNo MGB400 03 MGB400 04 MagCore Genomic DNA Large Volume Whole Blood Kit cc a 9 Cartridge Code 104 CatNoMGB1200 MagCore Plasma DNA Extraction Kit 1 2 ml ince ue osa Us Roe e eere aie We de SOO Swe 0 9 a JE Keane Rea Re ORE e 11 Cartridge Code 105 CatNoMPD1200 MagCore Genomic DN A Whole Blood Kit For Genotyping P 13 Cartridge Code 106 catNo MGB400 07 MGB400 08 MagCore Cultured cells DNA Kit EEEE E S A A EIE E E EENE E A E E E E E E E EEE E T E 15 Cartridge Code 110 Cat NoMCC 01 MCC 02 MagcCore Viral Nucleic Acid Extraction Kit 0 000s cece teenie nnn eens 17 Cartridge Code 201 Cat No MVN400 01 MVN400 02 MagCore Viral Nucleic Acid Extraction Kit oen 19 Cartridge Code 202 Cat No MVN400 03 MVN400 04 MagCore Viral Nucleic Acid Large Volume Extraction Kit 2 4 ml vn 21 Cartridge Code 210 CatNo MVN2400 MagCore Viral Nucleic Acid Large Volume Extraction Kit 1 2 ml 6n 23 Cartridge Code 211 CatNo MVN1200 MagCore Genomic DNA Plant Kit ree hehehe neenon 25 Cartridge Code 301 CatNoMGP 01 MGP 02 MagCore Genomic DNA Tissue Kit e MM I 33 Cartridge Code 401 Cat NoMGT 01 MGT 02 MagCore Genomic DNA FFPE One Step KI 3e ee ee ee RAqMPRIM UD EOD M EMEN 35 Cartridge Code 405 MGF 01 MGF 03 MagCore Genomic DNA Bacterial Kit sd died dcs togio qud p ae v ieu ecce ecieres 6 oe NU E Gio erure ave sue Sie Wuaanel aa cenerave 39 Ca
5. 55318 Te 4 WT a ARR eee ee mee eT E 100 pcs Comer ACTED asiatiska 1 pcs RNase Free WOE acci iid ux ida ierimen Rui igus qM nini UE 1 pcs gus perg c e 2 pcs PK Storage BONED tcetienciccrtcentscitiechsclimetteesneacetaientaanmemeuietinainen 2 pcs 4 Shelf life 12 months Cartridge Contents 2 ae 3 S gt g a E gt g gt g gt g gt g rm rm o s m tA ta 5 SIS IBRI Ble 3 S5 5 3 E gs S S Ss 5 po N a a Q e o A 9 9 a e p 3 A gt gt gt gt 3 S 3 E Ss eis sie 12 9 SB SR gt gt 8 8 8 S 25 B5psisosi sm 9m 9 m S S E 3 S So So As S i 82 8 9 58 x zB gt Q EN Ss g ESB S S Su g B B S S Description MagCore Viral Nucleic Acid Extraction kit is designed to extract viral DNA and RNA via MagCore auto extraction instrument With all the kit components of plastic consumables are DNase RNase Free pretreated and individual processing track for each loaded samples this system eliminates all possible cross contamination between samples Built in protocol with flexibility in sample source volumes both DNA and RNA virus can be extracted using the same kit in a fast and economical way Applications Using magnetic particle technology to purify viral nucleic acid from serum plasma or cell free body fluids The purified viral nucleic acid is suit
6. Cat DNO036 or Cat DNO096 for genomic DNA treatment For product information please contact RBC Bioscience distributor We also recommend to use RNase free DNase I enzyme 1U ul of Novagen Cat 69 182 3 Please contact local Merck branch office or distributor for product information 1X DNase Buffer can be prepared as following 1X DNase reaction buffer 10 mM Tris pH7 6 2 5 mM MgCl 0 1 mM CaCl in DEPC water autoclave Fresh Whole Blood Protocol Without DNase I treatment Add 1 volume of human whole blood with 3 volumes of RBC lysis Buffer in an appropriately sized tube not provided and mix by inversion Do not vortex For example add 1200ul of RBC lysis Buffer to 400ul of whole blood 2 Incubate the tube for 10 minutes on ice and invert 2 3 times during incubation 3 Centrifuge for 3 minutes at 500 x g 2 500rpm at 4 C and completely discard the supernatant 4 Add 500ul RBC lysis Buffer to the cell pellet Resuspend cells by vortex briefly 5 Transfer the suspended cells to the MagCore Sample Tube 6 Centrifuge for 3 minutes at 500x g 2 500rpm at 4 C and completely discard the supernatant 7 Add 200ul RB buffer contain B ME to the white pellet and mix by vortexing can storage up to 1 month at 80 C 8 Place the prepared Sample Tube into well 4 of T rack 9 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 10 Run Code 601 program
7. 96 pcs Pipet Tip plus Holder SEE sisdutiutemasn tuni nn o iae md neue 100 sets Sample TUDEL using eiie anin bU ue SEN 100 pcs Elution TUBES casada RD DM NM 100 pcs Proteinase IT TMg sisiane 2 pcs PK Storage BUITER ess suuni es i ni bcm eb EE 2pcs Storage and Stability 1 This kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Shelf life 12 months Cartridge Contents EE e S S a ee gt g SS 5 x SIRIS I IPIS S JS es g9 g9 D po a a a a nw A 4 Sg 4 g g C C RA gt a zy iv S m S gt S S e D e S zx a8 a a S S is g Is m O m O m gt gt at zi S S X S 3 3 Q Q m m Zh S N 2a lS a X Sg z8 3 S S en e 2 amp NEN 8 8 8 8 8 2 7 S E ELBE ES Description MagCore Plasma DNA Extraction Kit is designed for purification of DNA from 1 2 ml of serum plasma cell free body fluids by using MagCore auto extraction instrument With all the kit components of plastic consumables are DNase RNase Free pretreated and individual processing track for each loaded samples this system eliminates all possible cross contamination between samples Built in protocol with flexibility in sample source volumes plasma DNA can be extracted using this kit in a fast and economical way
8. Applications The purified total nucleic acid is suitable for highly sensitive and quantitative PCR MagCore Plasma DNA Extraction Kit has been proven with various genomic analyses as downstream applications lon www rbcbioscience com MagCore Running Time 76 min sample volume 1200 Preparation before using 1 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Protocol 1 Pipet20 ul proteinase K 10mg ml into the MagCore Sample Tubes 2 Add 1200yl of serum plasma cell free body fluids into the prepared Sample Tube 3 Place the prepared Sample Tube into well 4 of T rack 4 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 5 Run Code 105 program at MagCore Introduction of each MagCore Nucleic Acid Extraction kit a ENTUM C MagCore Genomic DNA Whole Blood Kit For Genotyping Purify genomic DNA from human whole blood for genotyping Cartridge Code 106 Cat No MGB400 07 MGB 400 08 Kit Contents Check that the following parts are included in addition to the main unit Cat No MGB400 07 Contents Cat No MGB400 08 Contents Pre filled Cartridge Fleagant ni eo s tetris 36 pcs Pre filled Cartridge Reagelil sasio asit tene ERU D 96pcs Pipet Tip plus HORS BI recess nmi ene est mnt aurei rais 36 sets Pipet
9. Cartridge Code 110 Cat No MCC 01 MCC 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MCC 01 Contents Cat No MCC 02 Contents Pre filled Cartridge Reagent ese denosd eerste veer rip naigs 36 pcs Pre filled Cartridge REAGENT ses arenas Potente 96 pcs Pipet Tip plus Holder Set icta tatu matos 36 sets Pipet Tip plus Holder Sel neuste elution mnt 1005ets Sample 18 eee eee eee EE ineen OOO 36 pcs Sample TIS 100 pcs Pe gro V M ere 36 pcs UOTE e 100pcs Proteinase K DER esrin 1pcs Proteinase K TMO eaa ducite nini aue aliti epu a RE BR titu 2pcs PKStorage BHITEE eie instet p one iine 1 pcs PA StOr ge Bufer e cssubteiiatetontisiu mnes ian itm RM mm 2 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Shelflife 12 months Cartrige Contents E SSS SS JAN e E Eg E PL E E gs gs Vn 72 S S gs 5 R amp F B BS BS S S Eo E E zi hi SR S s B8 8 al RIS Aa S g N S 8 838 g S 8 8 M a S un a a E S S8 8 8 8 E E E E Description MagCore Cultured cells DNA Kit is designed to extract genomic DNA from up to 5x10 cultured cel
10. For purification of genomic DNA from human whole blood 1 2 ml Cartridge Code 104 Cat No MGB1200 Kit Contents Checkthatthe following parts are included in addition to the main unit Cat No MGB1200 Contents Pre filled Cartridge Fleagent auae dio Rieti tins 96 pcs Pipet Tip plus Holdet SOT ossis t obi Od ra ter EE aide 100 sets Sample po m M 100 pcs Elution Tube ates eee E cred 100 pcs Proteinase K TMI BRI RR 8pcs PK Stor ge Bufer accessu a SEU UR ANAE 8pcs Storage and Stability 1 This kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Shelf life 12 months Cartridge Contents E s Nooo O HPP L HEM 0 91D28H CIM 40 9 102H IMooz 4ayng sis L IEM uonpipdag spb q2n uboy L IEM uonpipdag spbaq Jauboyy NOOO 4ayng uonn 3 MOOS 1 zayng Z usoM MOOS 1 Jagng YSOM i000 L ainyxiyy sppag Moos 4eyng buipuig Description MagCore Genomic DNA Large Volume Whole Blood kit is designed to extract genomic DNA from 1 2ml fresh whole blood via MagCore auto extraction instrument The kit contains all required reagent and labware for automated purification using magnetic particle technology Easy select program code number 104 in MagCore and combine using MagCore Genomic DNA Large Volume Whole Blood Kit can extract h
11. Holder Set into well 2 of T Rack 7 Run Code 202 program at MagCore MagCore Viral Nucleic Acid Large Volume Extraction Kit MagCore Viral Nucleic Acid Large Volume Extraction Kit 2 4 ml For extraction of viral DNA RNA from large volume 2 4 ml serum plasma and cell free body fluids Cartridge Code 210 Cat No MVN2400 Kit Contents Checkthatthefollowing parts are included in addition to the main unit Cat No MVN2400 Contents Pre filled Cartridge REGO ss usc spit bari mitt dade 96 pcs Pipet Im plus Holder Sel edes nEtieims 100 sets aum SONNE JUDB s censent wu natn enn ieiunus 100 pcs IP Te 2 EI P E 100 pcs Camer ACT aaa aeta a uoa ot eU ue mies e EE EE 2 pcs kNase free VV NCI c isnasorsttiR ndapurtl hia pinu DNE ide 2pcs FIOfEIDGISE K DIOE as secius tais apa aun claciSo bna 4 pcs PRAOIDIDSPBUITEI cn cisosstide sismo ut emet EI NR 4pcs Storage and Stability 1 This kit should be stored at room temperature 2 Carrier RNA should be stored at 20 C when mixing with RNase Free Water 3 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 4 Shelflife 12 months Cartridge Contents lt L aee 5e e N m m So gt wD t t S m 3 188 8 S S R PF Ss F gs gs Es Q o o D D a a Qa e Q S S A Q gt A A 3 gt a gt E a gt 3 etg z lasl SF ele eg S g Sm S Q 3
12. Nucleic Acid Extraction Kit has been proven with HBV HCV HIV and influenza viruses for downstream applications e www rbcbioscience com MagCore Running Time 57 min sample volume 200 ul 66 min sample volume 400 ul Preparation before using 1 Add 1 0 mI RNase Free Water to the Carrier RNA tube and mix by vortexing Store prepared Carrier RNA 1mg ml at 20 C 2 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Protocol 1 Pipet 10 ul Carrier RNA 1mg ml and 20 ul proteinase K 10mg ml into the MagCore Sample Tubes 2 Add 200 ul or 400 ul of serum plasma or cell free body fluids into the prepared Sample Tube 3 Place the prepared Sample Tube into well 4 of T rack 4 PuttheElution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 5 Run Code 202 program at MagCore Urine Protocol Sample preparation 1 Harvest cells from up to 3 5 ml urine by centrifugation for 10 minutes at 3000 rpm and concentrate the sample to 400 ul 2 Add 5 10 ul ProteinaseK 10mg ml to the sample Vortex for 5 seconds to mix sample 3 Incubate at 56 C for 10 minutes until the sample lysate is clear During incubation invert the tube every 3 minutes 4 Pipet 10 ul Carrier RNA 1mg ml into the MagCore Sample Tubes 5 Place the prepared Sample Tube into well 4 of T rack 6 Putthe Elution Tube into well 1 of T Rack and the Tip Plus
13. Protocol 1 Pipet 20 ul Carrier RNA 1mg ml and 40 ul proteinase K 10mg ml into the 5ml Sample Tubes provided 2 Add 2400 ul of serum plasma or cell free body fluids into the prepared 5ml Sample Tube 3 Place the prepared 5ml Sample Tube into well 4 of T rack for 5ml Sample Tube 4 Putthe Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 5 Run Code210 program at MagCore MagCore Viral Nucleic Acid Large Volume Extraction Kit ERU UEM MagCore ViralNudeicAddLarge VolumeExtractionKit 1 2 ml For extraction of viral DNA RNA from large volume 1 2 ml serum plasma and cell free body fluids Cartridge Code 211 Cat No MVN1200 Kit Contents Checkthatthefollowing parts are included in addition to the main unit Cat No MVN1200 Contents Pre filled Cartndgefiedgent su tee ennt mrt tun 96 pcs Pipet I plus Holder Set s bete te eei ane Sinon Eis 100 sets CT ro Kc T 100pcs Elution Ss T OP 100 pcs Camer RNA DIG PR H 1 pcs RNase Hee Watersiin 1pcs Proteinase KO TMO eset cc aaa A 2 pcs PK STADE Buffel rianan a n ERR p 2pcs Storage and Stability 1 This kit should be stored at room temperature 2 Carrier RNA should be stored at 20 C when mixing with RNase Free Water 3 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 4 Shelflife 12 months
14. Vortex for 15 seconds and centrifuge sample at full speed 13 000rpm for 1 min to pellet stool particles Pipet 400ul of the supernatant into a new 1 5ml microcentrifuge tube Add 20ul Proteinase K 10mg ml to the sample mixture and vortex to mix Incubate at 60 C for 2 3 hours to lyse the sample If there are any insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5min to get clear tissue solution in the Collection Tube Pipette 400ul of clear tissue solution to the MagCore Sample Tube Place the prepared Sample Tube into well 4 of T rack 10 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 11 Run Code401 program at MagCore pi www rbcbioscience com MagCore For Feed soil Sample Uni 6 O NA Apply 30 40mg feed or soil samples into a 1 5 ml microcentrifuge tube Add 20ul 10mg ml Proteinase K and followed by adding 500ul of GT Buffer Vortex gently until the powder suspend in GT buffer Incubate the mixture at 56 C for 15mins Invert the tube every 2 3mins during incubation Typically 15mins incubation can lysis more than 90 cells Extend incubation time to 20mins can increase 10 of yield Centrifuge the mixture for 3 min at full speed Ifthere are any insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5min to get clear tissue solution
15. at MagCore and select Remove Genomic DNA 2 NO With DNase treatment 1 Follow step 1 9 of without DNase treatnent protocol to prepare whole blood cell sample Soo hy 2 Besure to place the 200ul DNase mixture in 1 5 ml screw tube A e ces zu into the well 3 of T Rack gt oe awe x gt 3 RunCode 601 program at MagCore and select Remove Genomic pes DNA 1 YES es We Well Elution Tube Tip Tip Holder DNase Sample Tube MagCore Total RNA Cultured Cells Kit MagCore Total RNA Cultured Cells Kit For total RNA extraction from cultured cells Cartridge Code 610 Cat No MRC 01 MRC 02 Forisolation of total RNA from animal tissues and FFPE samples the MagCore total RNA Cultured Cells Kit is a relatively suitable choice and functions with a special protocol In the near future a more unique and convenient reagent will be developed for the extraction of RNA from animal tissue and FFPE samples Kit Contents Check that the following parts are included in addition to the main unit Cat No MRC 01 Contents Cat No MRC 02 Contents Pre filled Cartridge RECOGNI uu es eei ein nur 36pcs Pre filled Cartridge REGGE aussi esi itn sti ES 96 pcs Pipet Tip plus Holders nne ottiene ER iis 36 sets Pipet Im plus Holdet SO ssc iets oin utente etin et 100 sets Sample a 36 PCs Sample TUDE cennsa On 100pcs Elation Tube ananena E Ee 36pcs Elution TUDE iseis EEn as 100pcs RB Bufter 15M unnie eei ort trt
16. downstream application such as quantitative PCR restriction enzyme digestion southern blotting etc www rbcbioscience com MagCore Running Time 44 min sample volume 200 ul 57 min sample volume 400 ul Whole Blood Protocol 1 Pipet 200 400yul of equilibrated whole blood sample to MagCore Sample Tube 2 Puttheprepared Sample Tube into well4 of T Rack 3 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 4 Run Code 101 program at MagCore Optional Step RNA Degradation If RNA Free genomic DNA is required perform these optional steps 1 Add 4ul RNase A 50mg ml into the sample lysate 2 Incubate the sample at room temperature for 20min Buffy Coat modify Protocol RBC Lysis Buffer 150 mM NH CI 10mM KHCO 0 1mM EDTA Buffy Coat Preparation by RBC Lysis 1 Take600 700ul whole blood into 2m microcentrifuge tube Don t take more than 700ul whole blood sample it will cause the leakage situation during process Add 1ml RBC Lysis Buffer and mix the buffer and whole blood sample by upside down Shake the mixture 100rpm 5mins Centrifuge the mixture 13 000rpm 1 min Discard supernatant Repeat step 2 step 5 to wash the sample again Add 400ul RBC Lysis Buffer and 20yl proteinase K to resuspend the pellet and transfer into MagCore Sample Tube Puttheprepared Sample Tube into well 4 of T Rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder S
17. lime 1pcs RB Buffer G0ml attire ttt etti totes mette 1 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Shelf life 12 months Cartridge Contents SO E 8 89798 B8 P FILS es 8 S S a a uv D a a a o e S S S 4 e z gt gt A A gt 2 gt Oy S o3 S gt 2 SE No U No c amp n amp n S S S 2 amp 2 R P PIS S8 P P x eg s S A Sm Zh Zh Q Q zh zh e Zh a S m JN g S lft S8 S5 E 3 8 8 Un ge SR N S Ss s S 8 S S S N S e E amp S S ps d amp Description MagCore Total RNA Cultured Cells Kit is specially designed for total RNA purification from up to 1x106 cultured cells by using MagCore auto extraction instrument The program provides optional protocol for contaminated genomic DNA remove Combine RBC high quality RNase free DNase I with MagCore Total RNA Cultured Cells Kit can provide high quality DNA free total RNA Applications Using magnetic particle technology to purify total RNA The purified RNA can be directly used for downstream application such as real time PCR RT PCR cDNA synthesis etc D ee 2 ex S De ee ow E Well Elution Tube Tip Tip Holder MEM www rbcbioscience com DNase Sample Tube MagCore Running Time 42min without DNase treatment start
18. lysed A If there are any insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5 min to get clear tissue solution in the collection tube Pipette 400ul of clear tissue solution to the MagCore Sample Tube Place the Sample Tube into well 4 of T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack Run Code 401 program at MagCore ON Q9 uU Introduction of each MagCore Nucleic Acid Extraction kit MagCore Genomic DNA FFPE One Step Kit For extraction of total DNA from formalin fixed paraffin embedded FFPE tissue by using MagCore System Cartridge Code 405 Cat No MGF 01 MGF 03 Kit Contents Checkthatthe following parts are included in addition to the main unit Cat No MGF 01 Contents Cat No MGF 03 Contents Pre filled Cartridge REGGE wis ui eme iiit ritis 36 pcs Pre filled Cartridge REGGE aca csraecacrnaicuictecauncnatiemamuss 72pcs Pipet Tip pl s Holder Seba ideas renes tem dine 72sets Pipet Tip plus Holder Sel acuintedici mne petcnsetti detiene 150sets El tion WP a eee mee eee ae ene ene ere cee re 36 pcs Elution TUDE sesanan ne ee ee ee ee UR er RUD 75 pcs 17 10 er H M 25 ml L7 p mr M S 50ml Proteinase KO TMO aaasieitn tuin ae Ea tiU ecc ERR RR E GNIS 1 pcs Proteinase KTM m 2 pcs PK Storage BUITER eias e PR DERE e cca iud ua mai im EiS TE 1
19. of outer paper from the end of the cigarette or filter Cut this piece into 6 smaller pieces Transfer the pieces toa 1 5 ml microcentrifuge tube Cell Lysis 1 Add 500ul GT buffer and 20yl Proteinase K close the lid and mix for 10 sec Incubate at 60 C for 1 hour to lyse the sample 2 Briefly centrifuge the tube to remove drops from the inside of the lid 3 Pipette 400ul of clear tissue solution to the MagCore Sample Tube 4 Place the prepared Sample Tube into well 4 of T rack 5 Putthe Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 6 Run Code401 program at MagCore Hair roots Protocol Sample preparation 1 Cut the hair roots into 0 5 1 cm pieces and transfer them to the 1 5 ml microcentrifuge tube Cell Lysis 1 Add 500ul GT buffer and 20yl Proteinase K close the lid and mix for 10 sec Incubate at 60 C for 1 hour to lyse the sample 2 Briefly centrifuge the tube to remove drops from the inside of the lid 3 Pipette 400yul of clear tissue solution to the MagCore Sample Tube 4 Place the prepared Sample Tube into well 4 of T rack 5 Putthe Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 6 Run Code401 program at MagCore www rbcbioscience com MagCore Chewing Gum Protocol Sample preparation 1 Cutupto 30 mg of chewing gum into small pieces and transfer them to a 1 5 ml microcentrifuge tube Cell Lysis 1 Add 500ul GT
20. sample volume 1200 ul Preparation before using 1 Add 1 0ml RNase Free Water to the Carrier RNA tube and mix by vortexing Store prepared Carrier RNA 1mg ml at 20 C 2 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Protocol 1 Pipet 1Oul Carrier RNA 1mg ml and 20 ul proteinase K 10mg ml into the MagCore Sample Tubes provided 2 Add 1200 ul of serum plasma or cell free body fluids into the prepared Sample Tube 3 Place the prepared Sample Tube into well 4 of T rack 4 Putthe Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 5 Run Code21 1 program at MagCore Introduction of each MagCore Nucleic Acid Extraction kit Es nth MagCore Genomic DNA Plant Kit For extraction of genomic DNA from plant and fungal tissues Cartridge Code 301 Cat No MGP 01 MGP 02 Kit Contents Checkthatthefollowing parts are included in addition to the main unit Cat No MGP 01 Contents Cat No MGP 02 Contents Pre filled CarnmdaeFIeOgellt usecennsm lotam verat e d 36 pcs Pre filled Cartridge REGEN os aoniosorett et renter 96 pcs Pipet Tip plus Holder SOF sce cscs iiec iav iE dune 36 sets Pipet Tip plus Holder Sep tete eb m emeuE ls 100 sets Sample Te as ustusueuduus Eabb eee 36 pcs Sample 0 cs a CeO Oe UM M RENE TO 100 pcs Elution s t Ree tee ne ee ae IR 36 pcs Elution EI o NE E I Rm 100 pcs
21. 50mL NALC NaOH solution 2 Mix 10mL specimen with 10mL NALC NaOH sol RT C for 15 min 3 Add 25mL PBS mix and centrifuge 3000 x g for 15 min 4 Discard supernatant resuspend pellet with 200ul Lysozyme solution and transfer to the MagCore Sample Tube 5 Incubate for at least 30min at 37 C During incubation vortex the tube every 5min Cell Lysis Add 4ul RNase A 50mg ml to sample mixture including any precipitate and vortex to mix sample Incubateat room temperature for 10min Resuspend sample mixture by pipetting Adding 40ul Proteinase K 10mg ml to sample mixture and vortex to mix sample Place the prepared Sample Tube into well 4 of T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack Run Code 502 program at MagCore N 9 WH KR WN gt General Protocol Harvest bacteria maximum 2 x 10 cells into the MagCore Sample Tube by centrifuging at 5000 x g 8000rpm for 3min Discard supernatant Resuspend bacterial pellet in 200ul Lysozyme solution by vortexing or pipetting Incubate for at least 30min at 37 C During incubation vortex the tube every 5min Add 4ul RNase A 50mg ml to sample mixture including any precipitate and vortex to mix sample Incubate at room temperature for 10min Resuspend sample mixture by pipetting Adding 40ul Proteinase K 10mg ml to sample mixture and vortex to mix sample Place the prepared Sample Tube into well 4 o
22. Filter Col mm Sela s e minia E 36 pcs Filter Colunn Go aera meen eens ere area MM an M mE 100 pcs RNase A T OM GAN 275 ansia nim vmi Fr mE eR ERU mci PEU 1 pcs RNase A T OMIGATA 5501 seins munit reuicisimete pa Rut Eee 1 pcs GPT Buffer25ml n tiet i be t epe 1 pcs GPT Buffer 5Oml esset ttstsseettasett ttn tse 1 pcs GP2 Buffer GI eseossssssssssssossossensssssnsssossnsssssssunssssssssssssassssssnsssossusnsssvnsssesssneee 1 pcs GP2 BUPFCr T SIT cessoosssssssssssssessosssssssssnssssesssssssscnsssssssssssssenssssvnssssssssssssvnsssssee 1 pcs Storage and Stability 1 This kit should be stored at room temperature 2 For long term storage RNase A should be stored at 4 C 3 Shelf life 12 months Cartridge Contents lt L SS SS Se gt E SS x 7 3 2 8 8 3 5 8 gs 3 3 3 3 S o R 2 S 2 S S S S g g lt lt 5 O W zA amp D Sm Sm A ET S S S eS S S 3 gc 3 c a m m c h un a SOS S 8 8 38 fF 8g ZS 5 N S S S en s S 3 ike S E EAEAEL Description MagCore Genomic DNA Plant Kit is designed for purification of DNA from plant tissues and cells by using MagCore auto extraction instrument The provided Filter Column Set can filtrate hard tissue sample and prevent tissue residues to obstruct pipette tip during the process of MagCore The kit contains all r
23. MagCore Nucleic Acid Extraction Kit User s Manual MagCore Automated Nucleic Acid MagCore HF48 MagCore Super MagCore HF16Plus MagCore Nudeic Add Extraction Kits 96 Tests MagCore Nucleic Acid RBC Bioscience Corp 3F No 132 Ln 235 Baogiao Rd Xindian Dist New Taipei City 23145 TAIWAN TEL 886 2 8912 1200 FAX 886 2 8912 1300 Ver 2014 1 REP Wellkang Ltd www CE marking eu www rbcbioscience com 29 Harley St London W1G 9QR UK Q dg RBCBioscience RBC Bioscience Corp 3F No 132 Lane 235 Baoqiao Rd Xindian Dist New Taipei City 23145 Taiwan TEL 886 2 8912 1200 FAX 886 2 8912 1300 http www rbcbioscience com MagCore Nucleic Acid Extraction Kit MagCore User s Manual CONTENTS Precautions rmp 1 Product selection guide WAR RR EEEE PEU M P KRVPI OR SERB OAK RAN PRPEA I E OO E0044 0404S OSORNO OSS UA PES 2 Howto Use KIE oco eee TUE Ua ee re er Tee ee rr nep REO re Cee eee errr eee ee ee E Ta TIE 3 Introduction of each MagCore Nucleic Acid Extraction kits Kit Contents Description Applications Pretreatment Protocol MagCore Genomic DNA Whole Blood Kit Speedy installation 1918 sim Esc UR E 9 E RUE 19 lanier sje 8 9 8 alte Ghee ese DURER UICE 6 5 Cartridge Code 101 Cat No MGB400 01 MGB400 02 MagCore Genomic DNA Whole Blood Kit
24. PK Storage Buffer 44 min sample volume 200 ul up to 5 x 10 cells 45 min sample volume 200 ul 57 min sample volume 200 ul d 66 mi le volume 400 ul HSM ees jai RNase Free DNase I Lyophilized 1500 Kunitz units x 1 vial 1 ml RNase Free Water x1 DN036 90 min sample volume 2400 ul 15 ml DNase Reaction Buffe DNase Set Uo Uo 73 min sample volume 1200 ul RNase Free DNase Lyophilized 1500 Kunitz units DN096 x 2 vial 1 ml RNase Free Water x2 30 ml DNase I Reaction Buffer 33 min sample volume 400 ul Uo 33 min sample volume 400 ul Uo O Uo Oo Ov Oo 44 min sample volume 200 ul O 42min without DNase treatment 68min with DNase treatment sample volume 200 ul Uo Ox 42min without DNase treatment 73 min with DNase treatment sample volume 200 ul ME vwww rbcbioscience com Note JE Www rocbioscience com Note MagCore
25. S eo i ow ES ss 9 m S Rm A T T S S eS S S S 5o 5o g z Jal EJE 3 el Fs 8 8 G G S S en a d S DA DA S S8 8 8 8 S s PE s T8 la EL e T g g Description MagCore Viral Nucleic Acid Large Volume Extraction Kit 2 4ml is designed for purification of DNA and RNA from 2 4 ml serum plasma cell free body fluids by MagCore auto extraction instrument With all the kit components of plastic consumables are DNase RNase Free pretreated and individual processing track for each loaded samples this system eliminates all possible cross contamination between samples Built in protocol with flexibility in sample source volumes both DNA and RNA virus can be extracted using the same kit in a fast and economical way Applications Using magnetic particle technology to purify viral nucleic acid from serum plasma or cell free body fluids The purified viral nucleic acid is suitable for highly sensitive and quantitative PCR MagCore Viral Nucleic Acid Large Volume Extraction Kit has been proven with HBV HCV HIV and influenza viruses for downstream applications www rbcbioscience com MagCore Running Time 90 min sample volume 2400 ul Preparation before using 1 Add 1 0ml RNase Free Water to the Carrier RNA tube and mix by vortexing Store prepared Carrier RNA 1mg ml at 20 C 2 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C
26. T T S EN a amp P 2g Ig AS SITS lJa a F 8 8 I 18 8 8 8 818 8 81 TU pe izgl i Description MagCore Total RNA Whole Blood Kit is specially designed for total RNA purification from up to 400ul human whole blood by using MagCore auto extraction instrument The program provides optional protocol for contaminated genomic DNA remove Combine RBC high quality RNase free DNase with MagCore Total RNA Whole Blood Kit can provide high quality DNA free total RNA Applications Using magnetic particle technology to purify total RNA The purified RNA can be directly used for downstream application such as real time PCR RT PCR cDNA synthesis etc www rbcbioscience com MagCore Running Time 42min without DNase treatment starting volume 200 ul 68min with DNase treatment starting volume 200 ul Preparation before using 1 B Mercaptoethanol B ME not provided must be added to RB Buffer before use Add 10ul of B ME per 1 ml of RB Buffer 2 Recommended Step DNA residue degradation Prepare DNase RNase free working solution according to the table below Add 10ul DNase with 190ul DNase reaction buffer 1X in the 1 5 ml screw tube not provided and place it into well 3 of T Rack Healthy Whole blood DNase DNase buffer 1X Up to 400 ul 10 ul 190 ul 3 RNase free DNase lis not including in MagCore total RNA Whole Blood Kit we recommend to use RBC RNase free DNase I
27. Tip plus Holger SO satum caben edulis 100 sets Sample TED es auci oou bb Cusen tula adi ees 36 pcs Sample TUDES eee Le e eni iA nete EE 100 pcs T 8 76 1g TUDE BRUN NEN RINT I RR NR RN 36pcs TS OT TERT o CIS IIR a 100 pcs Proteinase NU TIO anurteccitinun um Ge imniYHUU A 2 pcs Proteinase KUTO M c 4 pcs PK Storage Buffel cst en D SEE iudi ena E HC 2pcs PROSIDIUUBBETER cet ee eR EER UNCLE sau Cee eee 4 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Shelf life 12 months Cartridge Contents SSS sg E ae a SS A I I m m m Q ive ies T m n n ANE 8 8 8 ELBIBIBIR B8 8 8 Seg S S olS5 2 zZ a B5 Sm 383 RR S I S EJE ES T S g 3 8 S S e e EI zm amp at a m S S ca ca x NS 8 S S S S e i e E E ERRIS Description This kit is designed for genotyping application you can get completed gDNA from eluent We modify the reagent components and machine operation to make kit more suitable for genotyping The pre filled cartridge contains chaotropic salt and guanidine hydrochloride for cell lysis and protein degradation The chaotropic salt helps the strong binding of DNA and cellulose coated magnetic beads After the removal of contaminants the high quality DNA is eluted by low salt elution buffer or water Purified DNA of approximately 20 30 kb in length is suitable for genotyping or other applications Appl
28. able for highly sensitive and quantitative PCR MagCore Viral Nucleic Acid Extraction Kit has been proven with HBV HCV HIV and influenza viruses for downstream applications 17 www rbcbioscience com MagCore Running Time 45 min sample volume 200 ul 56 min sample volume 400 ul Preparation before using 1 Add 1 0ml RNase Free Water to the Carrier RNA tube and mix by vortexing Store prepared Carrier RNA 1mg ml at 20 C 2 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Protocol 1 Pipet 10 ul Carrier RNA 1mg ml and 20 ul proteinase K 10mg ml into the MagCore Sample Tubes 2 Add 200 ul or 400 ul of serum plasma or cell free body fluids into the prepared Sample Tube 3 Place the prepared Sample Tube into well 4 of T rack 4 Putthe Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 5 RunCode 201 program at MagCore Urine Protocol Sample preparation Harvest cells from up to 3 5 ml urine by centrifugation for 10 minutes at 3000 rpm and concentrate the sample to 400 ul Add 5 10 ul ProteinaseK 10mg ml to the sample Vortex for 5 seconds to mix sample Incubate at 56 C for 10 minutes until the sample lysate is clear During incubation invert the tube every 3 minutes Pipet 10 ul Carrier RNA 1mg ml into the MagCore Sample Tubes Place the prepared Sample Tube into well 4 of T rack P
29. at rrea M ee E 36 pcs L724 2 cal 05 e ee uni nii 100 pcs Elution TUDE MU R 36 pcs Elution TUDB sas cce meee ree never dui 100 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Shelf life 6 months Cartrige Contents N V W m EE g Z ZI gs 8 S18 S amp 5 amp 8 2 Sg SS gs S 8 m E pere Se Es BR BR S S Oo bur to D Uy a oe S mw Q Q ER Q S C AX c Q fb So 35 c c S i Ei S ah en eS D D o Q gt e 2 ES amp S T Un amp S U NO S S S 2 gt E E Za S e e S EAR AT S Description MagCore Genomic DNA Whole Blood Kit is designed for purification of total DNA including genomic mitochondrial and viral DNA from whole blood plasma serum buffy coat by using MagCore auto extraction instrument The method uses pre filled cartridge contains proteinase K and chaotropic salt to lysis cells and degrade protein DNA will bind to cellulose coated magnetic beads After washing off the contaminants the purified DNA is eluted by low salt elution buffer Purified DNA of approximately 20 30 kb in length is suitable for PCR or other enzymatic reactions Applications Using magnetic particle technology to purify genomic DNA from fresh whole blood The purified genomic DNA can be directly used for
30. blefor PCR or other enzymatic reactions Applications Using magnetic particle technology to purify genomic DNA from fresh whole blood The purified genomic DNA can be directly used for downstream application such as quantitative PCR restriction enzyme digestion southern blotting etc www rbcbioscience com MagCore Running Time 44 min sample volume 200 ul 57 min sample volume 400 ul Preparation before using 1 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Whole Blood Protocol 1 Takeanew Sample Tube and add 20yl of Proteinase K 10mg ml to 200ul of equilibrated whole blood sample 40ul Proteinase K to 400ul whole blood 2 Place the prepared Sample Tube into well 4 of T Rack 3 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 4 Run Code 102 program at MagCore Optional Step RNA Degradation If RNA Free genomic DNA is required perform these optional steps before adding Proteinase K 1 Add 4ul RNase A 50mg ml into the sample lysate 2 Incubate the sample at room temperature for 20min Buffy Coat modify Protocol RBC Lysis Buffer 150mM NH CI 10mM KHCO 0 1mM EDTA Buffy Coat Preparation by RBC Lysis 1 Take 600 700ul whole blood into 2m microcentrifuge tube Don t take more than 700ul whole blood sample it will cause the leakage situation during process Add 1m RBC Lysis Buffer a
31. buffer and 20yl Proteinase K close the lid and mix for 10 sec Incubate at 60 C for 1 3 hours to lyse the sample 2 Briefly centrifuge the tube to remove drops from the inside of the lid 3 Pipette 400ul of clear tissue solution to the MagCore Sample Tube 4 Place the prepared Sample Tube into well 4 of T rack 5 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 6 RunCode401 program at MagCore Betel Nut Residue Protocol Sample preparation 1 Cutupto 30 mg of betel nut residue into small pieces and transfer them to a 1 5 ml microcentrifuge tube Cell Lysis Add 500ul GT buffer and 20ul Proteinase K close the lid and mix for 10 sec Incubate at 60 C for 1 3 hours to lyse the sample Briefly centrifuge the tube to remove drops from the inside ofthe lid Pipette 400ul of clear tissue solution to the MagCore Sample Tube Putthe Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 1 2 3 4 Place the prepared Sample Tube into well 4 of T rack 5 6 RunCode401 program at MagCore Saliva Protocol Sample preparation 1 Forsaliva sample donor should not ingest anything for at least 30min prior to sample collection 2 Prepare PBS Buffer and 15 ml tube Cell Lysis 1 Apply the 1 ml saliva and add 4 ml PBS buffer not provided 2 Centrifuge at 1800xgfor 5 min and then carefully discard the supernatant 3 Resuspend the pellet in 400ul GT b
32. d tissue to small pieces and put into a microcentrifuge tube Add 400ul GT Buffer and 20ul Proteinase K 10mg ml to the tube and mix by vortexing Incubate at 55 C for 90min until the sample has been completely lysed If there are any insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5min to get clear tissue solution in the Collection Tube Pipette 400ul of clear tissue solution to the MagCore Sample Tube Place the prepared Sample Tube into well 4 of T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack Run Code401 program at MagCore For Stool Sample N AUA A 8 9 Additional Requirements Microcentrifuge Tube Weight 180 200mg stool in a 2ml microcentrifuge tube and place on ice Ifthe sample is liquid pipet 200ul into microcentrifuge tube Cut the end of pipet tip to make pipetting easier If the sample is frozen use a scalpel or spatula to scrape bits of stool into microcentrifuge tube on ice Add 1 5ml GT Buffer to sample Vortex continuously for 1 min or until the stool sample is thoroughly homogenized This is very importantto vortex sample thoroughly to ensure maximum DNA concentration in the final elutes Incubate the suspension for 5 min at 70 C This step can increase DNA recovery 3 5 fold if the sample target is Gram positive bacteria please increase to 95 C for cells lysis
33. e stored at 4 C 4 Shelf life 12 months Cartridge Contents gt ee lt Se Se Se JF y sl e ejslslrilels KE 3 S S 3 S S x ra e S S m S 9 8 9 S amp TS lt 5 o Nd nd 2 E P SR SR g Psi p s se B ga SR x A EN S a S e So S c s 2I ISLr Js LS 8 8 N S Slll l 28 SR E 8 j8 Description MagCore Genomic DNA Bacterial kit is designed to extract genomic DNA from both Gram and Gram bacteria via MagCore auto extraction instrument The kit contains all required reagent and labware for automated purification using magnetic particle technology Easy select program code number 502 in MagCore and combine using MagCore Genomic DNA Bacterial Kit can extract high quality genomic DNA Applications Using magnetic particle technology to purify genomic DNA from both Gram and Gram bacteria The purified genomic DNA can be directly used for downstream application such as quantitative PCR restriction enzyme digestion southern blotting etc ELM www rbcbioscience com MagCore Running Time 44min sample volume 200 ul Preparation before using 1 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C 2 Freshly prepared 20mg ml Lysozyme solution not provided with Lysozyme Reaction Buffer before use For Sputum Specimens Specimens Decontamination 1 Fresh prepare 0 5 NALC in 2 NaOH 1 5 Na Citrate solution Ex 0 25g NALC in
34. ed Sample Tube into well 4 of T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack Run Code 301 program at MagCore so 9 N Introduction of each MagCore Nucleic Acid Extraction kit MagCore P NRN Running Time 33 min sample volume 400 MagCore Genomic DNA Tissue Kit PIC DUNG Deore Lane Pains pote lel cia qi A SAEY aa paratcemueedac istum a 1 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Cartridge Code 401 For Paraffin Embedded Tissue Cat No MGT 01 MGT 02 Sample Preparation Additional Requirements Xylene or Substitutes Ethanol 96 10096 Microcentrifuge Tube Kit Contents Suggested Xylene Substitute A5597 Sigma Neo Clear Merck CitriSolv Fisher Check that the following parts are included in addition to the main unit 1 Slice small section 5 10um of paraffin em j nd transfer to a microcentrifi SEA MEE Oa ERG MEUS Conti Slice small section 5 10um of paraffin embedded tissue and transfer to a microcentrifuge tube Presfilled Cartridge Reagent c ntt cnini 36 pcs Pre filled Cartridge Reageh uuu nci piittiace 96pcs Discard the first 2 3 sections if the surface of paraffin sample has been exposed to air Pipet Tip plus Holder SOT ea ae dmi ane est mnt aurei rais 36 sets Pipet Tip pl
35. equired reagent and labware for automated purification using magnetic particle technology Easy select program code number 301 in MagCore and combine using kit can perform high quality genomic DNA Applications Using magnetic particle technology to purify genomic DNA up to 100mg of fresh tissue The purified genomic DNA can be directly used for downstream application such as quantitative PCR PCR southern blotting RADP AFLP etc EM www rbcbioscience com MagCore Running Time 33min sample volume 400 ul Preparation before using The kit procedures are optimized fora maximum of 100 mg of wet weight or 20 mg of dried starting material Exceeding the recommended maximum amount of starting material will result in inefficient lysis resulting in low yield and purity Tissue Dissociation Protocol 1 Cut50 mg up to 100 mg of fresh or frozen plant tissue or 5 mg up to 20 mg of dried sample 2 Grind the sample with mortar and pestle under liquid nitrogen to a fine powder For some plant samples liquid nitrogen may be unnecessary for homogenization 3 Transfer it into a microcentrifuge tube not provided Lysis Step Add 400yl GP1 Buffer and 5ul RNase A 10mg ml into the microcentrifuge tube and mix by vortexing Do not mix GP1 Buffer with RNase A before use 2 Incubate at 65 C for 10 minutes During incubation invert the tube every 5 minutes Add 100ul GP2 Buffer and mix by vortexing Incubate on ice fo
36. et into well 2 of T Rack TO Run Code 101 program at MagCore Buffy Coat Preparation by Centrifugation Take2 5ml whole blood sample and centrifuge at 1 500rpm 10mins Oo DAN WD WF KR W PN Use plastic drop to take white buffy coat layer in the middle of whole blood sample Move the buffy coat into new microcentrifuge tube Take 80 100ul buffy coat sample into MagCore Sample Tube and add RBC Lysis Buffer or PBS until 400ul then add 20ul of proteinase K A LU N oS Uni Puttheprepared Sample Tube into well 4 of T Rack 6 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 7 Run Code 101 program at MagCore Note We suggest to select 150 200I elution buffer it can get better elution efficiency in both of these methods Normally the concentration is higher than 150ng ul under such elution volume Introduction of each MagCore Nucleic Acid Extraction kit MagCore Genomic DNA Whole Blood Kit For purification of genomic DNA from human whole blood Cartridge Code 102 Cat No MGB400 03 MGB 400 04 Kit Contents Check that the following parts are included in addition to the main unit Cat No MGB400 03 Contents Cat No MGB400 04 Contents Pre filled Cartridge Feagant i u seo settimana 36 pcs Pre filled Cartridge Reageril ue acus iti trap RR SS 96 pcs Pipet Tip plus HORS BI aene dmn e
37. f T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 10 Run Code 502 program at MagCore 6 GAN HD NH KR W N Introduction of each MagCore Nucleic Acid Extraction kit a ee ACIE I M MagCore Total RNA Whole Blood Kit For total RNA extraction from human whole blood Cartridge Code 601 Cat No MRN 01 MRN 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MRN 01 Contents Cat No MRN 02 Contents Pre filled Cartridge Feageht ai eme stitit Etuis 36 pcs Pre filled Cartridge leagent sae etd imb SES 96 pcs Pipet Tip plus Holder ST e adt estt iere pto gen 36 sets Pipet Tip plus Holder SOL sesenta ppt puse titu name 100 sets Sample UD eae ae ee eT EU uU A inu SE 36 pcs LP 12310 cal 0 5 NINE naak Ren ee vO 100 pcs ST Ceo e 36pcs Elution TUDE Mop EA 100pcs RBG L sis Buffer 1 00M sssini 1 pcs RBC L sis BUNTEN DORIA aeniinctesu iste receta Rhodes iunt 1 pcs RB Buffer 15mm utt titt to eter s 1 pcs RB Buffer 30ml titii etit teet idees 1 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Shelf life 12 months Cartridge Contents ud Ece SS SS SS SS Q Oo ive ies ta t trf S EIS B E EZ ZR f8 18 Ss e ae S EI 2 z 2 S 5 S g S S pp pp 8 8 po po S E m Sm S m ET
38. ge tube 3 Add 5 10ul ProteinaseK 10mg ml to the sample Vortex for 5 seconds to mix sample 4 Incubate at 56 C for 10 minutes until the sample lysate is clear During incubation invert the tube every 3 minutes 5 Spin down the sample and apply for MagCore HF 16 Introduction of each MagCore Nucleic Acid Extraction kit MagCore Viral Nucleic Acid Extraction Kit For extraction of viral DNA RNA from serum plasma and cell free body fluids Cartridge Code 201 Cat No MVN400 01 MVN 400 02 Kit Contents Checkthatthe following parts are included in addition to the main unit Cat No MVN400 01 Contents Pre filled Cartridge Bedagehil osse tomos ins 36pcs Pinet Tip plis Holder SaL secuencias tano Sd metet 36 sets Sample TUb T 36 PCs Elution N90 eee ee ee ee eee ec enn ener 36 pcs Camer NACER eiren Eun diGq mI Go REG M MEA eU 1 pcs RNase Free VVOlek casa encasduticamii niin EBEN IH MEE Odin qM T 1 pcs Proteinase KT TMg RT 1 pcs PK Storage BUNT M 1 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Carrier RNA should be stored at 20 C when mixing with RNase Free Water 3 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer Cat No MVN400 02 Contents Pre filled Cartridge Reagent eese 96 pcs Pipet Tip plus Holder SEL isc crete ti tinto usps 100 sets Sample TUUS csset wtestu mA PINE UE ERE 100 pcs
39. ications Use magnetic particle technology to purify genomic DNA from whole blood and buffy coat The purified genomic DNA can be directly used for downstream application such as genotyping PCR real time PCR restriction enzyme digestion southern blotting etc EM www rbcbioscience com MagCore Running Time 44 min sample volume 200 ul 57 min sample volume 400 ul Preparation before using 1 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Whole Blood Protocol 1 Takeanew Sample Tube and add 20yl of Proteinase K 10mg ml to 200ul of equilibrated whole blood sample 40ul Proteinase K to 400ul whole blood 2 Place the prepared Sample Tube into well 4 of T Rack 3 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 4 Run Code 106 program at MagCore Optional Step RNA Degradation If RNA Free genomic DNA is required perform these optional steps before adding Proteinase K 1 Add 4ul RNase A 50mg ml into the sample lysate 2 Incubate the sample at room temperature for 20min Buffy Coat modify Protocol RBC Lysis Buffer 150mM NH CI 10mM KHCO 0 1mM EDTA Buffy Coat Preparation by RBC Lysis 1 Take 600 700ul whole blood into 2m microcentrifuge tube Don t take more than 700ul whole blood sample it will cause the leakage situation during process Add 1m RBC Lysis Buffer and mix the buffer and who
40. igh quality genomic DNA Applications Using magnetic particle technology to purify genomic DNA from 1 2ml fresh whole blood The purified genomic DNA can be directly used for downstream application such as quantitative PCR restriction enzyme digestion southern blotting etc a www rbcbioscience com MagCore Running Time 76 min sample volume 1200 ul Preparation before using 1 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C Protocol 1 Pipet Proteinase K 80 ul into the MagCore Sample Tubes 2 Add 1200 ul whole blood into the prepared Sample Tube 3 Place the prepared Sample Tube into well 4 of T rack 4 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 5 Run Code 104 program at MagCore Note Beads or precipitate in eluent might be happened in viscous samples This situation will not affect the yield purity and downstream applications Reduce volume of viscous sample or simply centrifuge will remove the residual beads Introduction of each MagCore Nucleic Acid Extraction kit rt MagCore PiasmaDNA Extraction Kit 1 2 ml For extraction of free circulating DNA from human plasma or serum Cartridge Code 105 Cat No MPD1200 Kit Contents Checkthatthe following parts are included in addition to the main unit Cat No MPD1200 Contents Pre filled Cartridge Reagent cierre te sete
41. in the Collection Tube Pipette 400ul of clear tissue solution to the MagCore Sample Tube Place the prepared Sample Tube into well 4 of T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack Run Code 401 program at MagCore For Dried Blood Spot Mm A LN Cut 3mm diameter punches from a dried blood spot with a single hole paper punch Place up to 3 blood card into a 1 5ml microcentrifuge tube Add 400 500ul GT buffer into the microcentrifuge tube and continue to homogenize the sample tissue with grinding Add 20ul Proteinase K 10mg ml to the sample mixture and vortex to mix Incubate at 60 C for 1 hour to lyse the sample Pipette 400ul of sample mixture to the MagCore Sample Tube Ifthereare any insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5min to get clear tissue solution in the Collection Tube 6 Place the prepared Sample Tube into well 4 of T rack N Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack Run Code 401 program at MagCore Optional Step RNA Degradation If RNA Free genomic DNA is required perform these optional steps before adding Proteinase K 1 Add Aul RNase A not provided 50mg ml into the sample lysate 2 Incubatethe sample at room temperature for 20min Cigarette Butts Protocol Sample preparation 1 Cut 1 cm piece
42. ing volume 200 ul 73min with DNase treatment starting volume 200 ul Preparation before using 1 B Mercaptoethanol B ME not provided must be added to RB Buffer before use Add 10ul of B ME per 1 ml of RB Buffer 2 Recommended Step DNA residue degradation Prepare DNase I RNase free working solution according to the table below Add 10ul DNase with 190ul DNase reaction buffer 1X in the 1 5 ml screw tube not provided and place it into well 3 of T Rack Cultured Cells 1X DNase Buffer Up to 1x10 10 ul 190 ul 3 RNase free DNase is not including in MagCore total RNA Cultured Cells Kit we recommend to use RBC RNase free DNase I Cat DNO036 or Cat DN096 for genomic DNA treatment For product information please contact RBC Bioscience distributor We also recommend to use RNase free DNase enzyme 1U ul of Novagen Cat 69 182 3 Please contact local Merck branch office or distributor for product information 1X DNase Buffer can be prepared as following 1X DNase reaction buffer 10 mM Tris pH7 6 2 5 mM MgCl 0 1 mM CaCl in DEPC water autoclave Cultured Cells Protocol Sample Preparation A Cells grown in suspension Cells grown in suspension up to 1 x 10 cells Determine the number of cells Transfer appropriate number of cells to the MagCore Sample Tube provided and centrifuge for 5 min at 300 x g Remove the supernatant completely and discard Continue with MagCore Operation step B Cells grown in a mono
43. l safety regulations RBC Bioscience does not warrant that samples treated with 3 N Lysis Buffer are completely inactivated and noninfectious After sample processing is completed s remove and autoclave all disposable plastics ib 2 2 5 z Shae E S5 e Donoteat drink or smoke in the laboratory working area mE Joh ESAE IR dh Wear protective disposable gloves laboratory coats and goggles when handling samples and kit reagents Do not use sharp or pointed objects when working with the reagent cartridges this is to prevent damage ofthe sealing foil and loss of reagent Do not contaminate the reagents with bacteria virus or ribonuclease Use disposable pipettes and RNase free pipette tips only to remove aliquots from reagent bottles Use the general precautions described in the literature Wash hands thoroughly after handling samples and test reagents IV Waste Handling Discard unused reagents and waste complied with country federal state and local regulations 101 MagCore Genomic DNA Whole Blood Kit Speedy installation 104 MagCore Genomic DNA Large Volume Whole Blood Kit 1 2 ml 202 MagCore Viral Nucleic Acid Extraction Kit Low PCR Inhibition 210 MagCore Viral Nucleic Acid Large Volume Extraction Kit 2 4 ml 211 MagcCore Viral Nucleic Acid Large Volume Extraction Kit 1 2 ml 106 MagCore Genomic DNA Whole Blood Kit For Genotyping MagCore Nudeic Acid Extraction Kits 102 MagcCore Gen
44. layer Cells grown in a monolayer up to 1 x 10 cells Cells grown in a monolayer can be detached from the culture flask by either trypsinization or using a cell scraper Totrypsinize cells Determine the number of cells Aspirate the medium and wash cells with PBS not provided Aspirate the PBS and add 0 10 0 2596 trypsin After cells have detached from the dish or flask collect them in medium and transfer the appropriate number of cells up to 1 x 10 cells to the MagCore Sample Tube provided Centrifuge for 5 min at 300 x g Remove the supernatant completely and discard taking care not to disturb the cell pellet Continue with MagCore Operation step Using a cell scraper Detach cells from the dish or flask Transfer the appropriate number of cells up to 1 x 10 cells to the MagCore Sample Tube provided and centrifuge for 5 min at 300 x g Remove the supernatant completely and discard taking care not to disturb the cell pellet Continue with MagCore Operation step MagCore Operation Without DNase treatment 1 Add 200ul RB buffer contain B ME to the cells pellet and mix by vortexing can storage up to 1 month at 80 C 2 Place the prepared Sample Tube into well 4 of T rack 3 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 4 Run Code 610 program at MagCore and select Remove Genomic DNA 2 NO With DNase treatment 1 Follow step 1 3 of without DNase treatnent protoc
45. le blood sample by upside down Shake the mixture 100rpm 5mins Centrifuge the mixture 13 000rpm 1 min Discard supernatant 2 3 4 5 6 Repeat step 2 step 5 to wash the sample again 7 Add 400yl RBC Lysis Buffer and 20ul proteinase K to resuspend the pellet and transfer into MagCore Sample Tube 8 Place the prepared Sample Tube into well 4 of T Rack 9 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack TO Run Code 106 program at MagCore Buffy Coat Preparation by Centrifugation Take2 5mlwhole blood sample and centrifuge at 1 500rpm 10mins Use plastic drop to take white buffy coat layer in the middle of whole blood sample Move the buffy coat into new microcentrifuge tube Take 80 100ul buffy coat sample into MagCore Sample Tube and add RBC Lysis Buffer or PBS until 400ul then add 20ul of proteinase K Nb N mS Uni Placethe prepared Sample Tube into well 4 of T Rack 6 PuttheElution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 7 Run Code 106 program at MagCore Note We suggest to select 150 200yl elution buffer it can get better elution efficiency in both of these methods Normally the concentration is higher than 150ng ul under such elution volume Introduction of each MagCore Nucleic Acid Extraction kit oe ed MagCore Cultured Cells DNA Kit For extraction of genomic DNA from cultured cells and amniotic fluid
46. ls via MagCore auto extraction instrument The kit contains all required reagent and labware for automated purification using magnetic particle technology Easy select program code number 110 in MagCore and combine using MagCore Cultured cells DNA Kit can extract high quality genomic DNA Applications Using magnetic particle technology to purify genomic DNA from 5x10 cultured cells The purified genomic DNA can be directly used for downstream application such as quantitative PCR restriction enzyme digestion southern blotting etc EW www rbcbioscience com MagCore Running Time 44 min sample volume 200 ul up to 5 x 10 cells Preparation before using 1 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 20 C 2 Ensure PBS buffer have been prepared for resuspend cell pellet Protocol Sample Preparation A Cells grown in suspension Cells grown in suspension up to 5 x 10 cells Determine the number of cells Centrifuge the appropriate number of cells for 5 min at 300 x g in a 1 5 ml microcentrifuge tube not provided Remove the supernatant completely and discard Continue with MagCore Operation step 1 B Cells grown in a monolayer Cells grown in a monolayer up to 5 x 10 cells Cells grown in a monolayer can be detached from the culture flask by either trypsinization or using a cell scraper To trypsinize cells Determine the number of cell
47. nd mix the buffer and whole blood sample by upside down Shake the mixture 100rpm 5mins Centrifuge the mixture 13 000rpm 1 min Discard supernatant 2 3 4 5 6 Repeat step 2 step 5 to wash the sample again 7 Add 400yl RBC Lysis Buffer and 20ul proteinase K to resuspend the pellet and transfer into MagCore Sample Tube 8 Place the prepared Sample Tube into well 4 of T Rack 9 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 10 Run Code 102 program at MagCore Buffy Coat Preparation by Centrifugation Take2 5mlwhole blood sample and centrifuge at 1 500rpm 10mins Use plastic drop to take white buffy coat layer in the middle of whole blood sample Move the buffy coat into new microcentrifuge tube Take 80 100ul buffy coat sample into MagCore Sample Tube and add RBC Lysis Buffer or PBS until 400ul then add 20ul of proteinase K Nb N Ss Uni Placethe prepared Sample Tube into well 4 of T Rack 6 PuttheElution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 7 Run Code 102 program at MagCore Note We suggest to select 150 200I elution buffer it can get better elution efficiency in both of these methods Normally the concentration is higher than 150ng ul under such elution volume Introduction of each MagCore Nucleic Acid Extraction kit M MagCore Genomic DNA Large Volume Whole Blood Kit
48. nd to cellulose coated magnetic beads After washing off the contaminants the Additional Requirements PBS Microcentrifuge Tube purified DNA is eluted by low salt elution buffer Purified DNA of approximately 20 30 kb in length is suitable for PCR 1 Separate the swab cotton form the stick Place the swab into a 2ml microcentrifuge tube add 500ul or more of GT Buffer and or other enzymatic reactions 20ul Proteinase K 10mg ml App lications 2 Incubate the sample lyaste at 55 C for 30min For Buccal Swab sample donor should not ingest anything for at least 30min prior to sample collection Using magnetic particle technology to purify genomic DNA from animal tissues paraffin embedded tissue swab and blood stain The purified genomic DNA can be directly used for downstream application such as quantitative PCR restriction enzyme digestion southern blotting etc 3 Ifthereareany insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5min to getclear tissue solution in the Collection Tube Pipette 400ul of clear tissue solution to the MagCore Sample Tube Place the prepared Sample Tube into well 4 of T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack Run Code 401 program at MagCore a www rocbioscience com N AUWA For Solid Animal Tissue Aa L N nm o N AD Additional Requirements Microcentrifuge Tube Cutthe soli
49. ol to prepare culture cell sample 2 Besure to place the 200ul DNase mixture in 1 5 ml screw tube into the well 3 of T Rack 3 Run Code 610 program at MagCore and select Remove Genomic DNA 1 YES MagCore Running Time List Ordering information Cat Number Reactions Code No Running Time Product Contents ciel MagCore Genomic DNA Whole Blood Kit MGB400 02 Speedy Installation MGB400 03 MagCore Genomic DNA Whole Blood Kit MGB400 04 MGB1200 MagCore Genomic DNA Large Volume Whole Blood Kit MCC 01 36 MagCore Cultured cells DNA Kit MCC 02 MVN400 01 MagCore Viral Nucleic Acid Extraction Kit MVN400 02 MVN400 03 MagCore Viral Nucleic Acid Extraction Kit MVN400 04 MVN2400 MagCore Viral Nucleic Acid Extraction Kit 2 4ml MVN1200 MagCore Viral Nucleic Acid Extraction Kit 1 2ml MGP 01 36 MagCore Genomic DNA Plant Kit MGP 02 MGT 01 MagCore Genomic DNA Tissue Kit MGT 02 MBB 01 MagCore Genomic DNA Bacterial Kit MBB 02 MRN 01 MagCore Total RNA Whole Blood Kit MRN 02 MRC 01 MagCore Total RNA Cultured cells Kit MRC 02 44 min sample volume 200 ul Accessories of HF16 57 min sample volume 400 ul Cartridge Rack MRCO01 3 3 6 6 44 min sample volume 200 ul T Rack For general using MRCO002 55 min sample volume 400 ul T Rack 5 ml sample tube For MVN2400 MRCO003 76 min sample volume 1200 ul Reagent 76 min sample volume 1200 ul 11 mg Proteinase K Proteinase K Set PK011 1 25 ml
50. omic DNA Whole Blood Kit 105 MagCore Plasma DNA Extraction Kit 1 2 ml 405 MagCore Genomic DNA FFPE One Step Kit 201 MagCore Viral Nucleic Acid Extraction Kit 110 MagCore Cultured Cells DNA Kit 301 MagCore Genomic DNA Plant Kit 401 MagCore Genomic DNA Tissue Kit 502 MagCore Genomic DNA Bacterial Kit Gy MagCore Total RNA Whole Blood Kit GQ Magcore Total RNA Cultured Cells Kit 3 Forisolation of total RNA from animal tissues and FFPE samples the MagCore total RNA Cultured more unique and convenient reagent will be developed for the extraction of RNA from animal Cells Kit is a relatively suitable choice and functions with a special protocol In the near future a tissue and FFPE samples Genomic DNA Viral Nucleic Acids Total RNA a www rbcbioscience com MagCore How to use kit MagCore HF 16 andCompact How to use kit MagCore HF48 Install all necessary accessories and apply your specimen to MagCore 2 3 48 Test MAGCORE 2 3 1 CARTRI DGE RACK 5 6 TUE E I 8 9 oa Extraction Kit 1 2 ml v i PRESS START TO RUN L1 EJ siege lier au SC 0 Enter SC 0 Enter Volume v M Sa Press START Atthis step prepare racks to operation area Elution Volume v B v After racks loaded then press Enter to next page for elution volume selecti
51. on 5 Back v 1 Pnitializing SELECT ELUTE VOL 1 OOOul 2 OOOul se e e When you finish input and press Start key the system will e e ev Je J eo ej e ev 3 000u 4 ooon show a confirmation window on which there are the ESC PREV PAGE parameters you input Please press Yes to operate thesystem After press Start button machine runs program of calibration ifthe parameters are not incorrect Please press No to modify initialize to move all axis to original factory positions Select final elution volume incorrect parameters ifyou want to modify v Md wn O O nett ESTERI Eie Cien aibi Press Start key in the main functional window 3 DI GI TAL S L3 Start i XXX DA 6 1 iin foetus cse E no Ps m 9 serene Enter Penam mu MagCore in process of selected protocol at this step The Green Indicate LED lights up and Heating Block starts to heat up to 65 C for Lysis Step m v While MagCore is under program running the MagCore LCD lights up atall times DONOT open the door at this moment it causes emergent stop m Remained time for system operation rogram 1 1
52. ot over 20mg of FFPE Introduction of each MagCore Nucleic Acid Extraction kit MagCore Genomic DNA Bacterial Kit For extraction of genomic DNA from bacteria Cartridge Code 502 Cat No MBB 01 MBB 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MBB 01 Contents Cat No MBB 02 Contents Pre filled Cartridge Fleagent uues eei 36 pcs Pre filled Cartridge FHeggahl asoa seo tdi tSt 96 pcs Pipet Tip plus Holder SET a ad ttam es tiere pto gd 36 sets Pipet Tip plus Holder SOL sounds ipusthtc titia mE 100 sets Sample TUB duds tune An ien UE 36 pcs Sample TL aaa ER RR 100 pcs Elution TUDE 36pcs Elution TUDE ccs csc caieheiec M 100 pcs Lysozyme Reaction Buffer 15ml s 1 pcs Lysozyme Reaction Buffer 30mnl eae 1 pcs Proteinase ROT TIR aaa caa iis to n Rn corte 2 pcs Proteinase NU Ig sec ieu Rn pA E Rma RE DR EE 4 pcs Ig Storage B ffel E IR on en 2pcs PK Storage 0 1 7 NR ee eee eee OT m 4 pcs RNase ACSI G T TOO uacua na t ttu t der rrt ni 1 pcs RNase A SUmmm ml dOOHUI sisisi 1 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 For long term storage RNase A should b
53. oth 2hrand 16hr program can extract DNA from FFPE sample Choose 2hr program for saving time choose 16hr for higher yield 2 Ifyou want to increase DNA yield an overnight incubation 16hr program can be performed but it may result in greater DNA fragmentation EP www rbcbioscience com Troubleshooting Symptoms Lowor NO DNA product Poor RCR results Clogging tip or liquid up to the tip filter MagCore Comments and suggestions The sample was lysed insufficiently Make sure the proteinase K was stored at 20 C and repeat the procedure using fresh PK The sample was too large to lyse completely The large FFPE tissue was suggested cutting into 4 sections and one scroll was enough for extraction Clogging tip will affect the extraction process Poor quality FFPE samples Fixation condition can affect PCR performance such as long time storage in fixative DNA fragments DNA purified from FFPE samples may be fragmented due to formalin fixation so we suggest keeping amplicons as shortas possible for PCR The sample was too large to pipetting Large tissue clogged the tip would result the liquid up to the tip filter or the extraction cannot finish We suggest cutting the tissue before adding in the Heat Block well 1 Thesample was too much to pipetting Do not extract too much scrolls ata time For large tissue one scrollis enough for extraction for small tissue we suggest n
54. pcs eie 8 816 2151 1 MN MER REN 2 pcs Thermo stable COI9Lss acies eee ee Cee TN EO eee 36 pcs Thermostable GUB Reem ee meee eater ae eee unn Dedi 75 pcs Storage and Stability vvv 1 This kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Shelf life 12 months Cartridge Contents SSeS EUER ee gt g Se gt m m m A ive w p O tA tA AEN 3 sS3 28 amp gs 89 53583 gs gs x 3 3 3 S e x R A 8 z g S Qs TPP sls s s ele l sy ss F S S Eo wo Uo S E zh Q S R 9 m Q As amp 8 S S 2s S S w m S zh ES e ET md e 3 gt gt g D EDLSIS Le ES ER ER E ELELS Description MagCore Genomic DNA FFPE One Step Kit is designed for purification of total DNA from FFPE tissues by using MagCore instruments It features the method One Step Heating to melt paraffin and lyse tissue samples at the same time without harmful reagents involved such as xylene Two protocols are designed and optimized for different sizes of tissues 2 hrs for small samples 16 hrs for large samples Please see important notes DNA will be extracted fastand economically based on the cellulose coated magnetic bead technology Applications Use magnetic particle technology to purify genomic DNA from FFPE tissue The purified genomic DNA can be directly used for downstream application s
55. r 3 minutes Place a Filter Column into a 2 ml Collection Tube and apply the entire lysate from previous step to the Filter Column a Ww en Centrifugefor 3 minutes at full speed 13 000 rpm O Discard the Filter Column and carefully transfer clarified lysate about 400 in the collection Tube to the MagCore Sample Tubes Place the prepared Sample Tube into well 4 of T rack Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 9 Run Code 301 program at MagCore 9o N Fungal Tissue Protocol Sample preparation 1 Collectthefungaltissue up to 20 mg 2 Grind the sample with mortar and pestle under liquid nitrogen to a fine powder 3 Transfer itinto a microcentrifuge tube not provided Do not allow the sample to thaw Cell Lysis Add 400u GP1 Buffer and 5ul RNase A 10mg ml into the microcentrifuge tube and mix by vortexing Do not mix GP1 Buffer with RNase A before use 2 Incubate at 65 C for 10 minutes During incubation invert the tube every 5 minutes Add 100ul GP2 Buffer and mix by vortexing Incubate on ice for 3 minutes Place a Filter Column into a 2 ml Collection Tube and apply the entire lysate from previous step to the Filter Column A Ww en Centrifugefor 3 minutes at full speed 13 000 rpm O Discard the Filter Column and carefully transfer clarified lysate about 400 in the collection Tube to the MagCore Sample Tubes Place the prepar
56. ridge Contents 8 Remove residual ethanol with a fine pipette tip then open the tube and incubate at 55 C for 5min until all residual ethanol has sS F e I S 3 31 gs gs 9 Add 400ul GT Buffer and 20ul Proteinase K 10mg ml to the tube and mix by vortexing 2 S E l EIIZ 2 go S m qm amp 8 Pal Ps SR SR 10 Incubate at 55 C for 90min until the sample has been completely lysed m S ci ci S E w is f a S ai S 8 Sa 1 1 If there are any insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5min to N r 5 E BIS 8 get clear tissue solution in the Collection Tube 12 Pipette 400yl of clear tissue solution to the MagCore Sample Tube 13 Place the prepared Sample Tube into well 4 of T rack 14 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 15 Run Code401 program at MagCore C Description MagCore Genomic DNA Tissue Kit is designed for purification of total DNA including genomic mitochondrial and viral DNA from a variety of animal tissues or cells by using MagCore auto extraction instrument The provided Filter Column can filtrate hard tissue sample or swab sample to prevent tissue residues to obstruct pipette tip during the process of MagCore The method uses pre filled cartridge contains proteinase K and a chaotropic salt to lysis cells ForSwab Sample and degrade protein DNA will bi
57. rtridge Code 502 CatNo MBB 01 MBB 02 MagCore Total RNA Whole Blood Kit 1 ci cE en ee een EES 41 Cartridge Code 601 Cat NoMRN 01 MRN 02 MagCore Total RNA Cultured Cells Kit eee BmwmSRSReemBRRVSeeewnees 43 Cartridge Code 610 Cat No MRC 01 MRC 02 Running Time List MagCore Precautions Product selection guide I Before Using Donotoperate MagCore without qualified operation training bw Read users manual carefully before operation y Il Handling Requirements N Donotuseakitafterits expiration date s gt gt Donottouch the reagents with bare hands Keep away from your skin eyes or mucous membranes b AN x x If contact does occur wash the affected area immediately with large amounts of water If you spill the e gt reagents dilute the spill with water before wiping it up LN gt gt Do not allow reagents to mix with sodium hypochlorite solution or strong acids This mixture can QD gt gt gt gt produce a highly toxic gas RS m x Ill Laboratory Procedures S gt gt Handle all samples and the resulting waste as if potentially infectious using safe laboratory Ew EF EN a ae JEJE TIF gt procedures As the sensitivity and titer of potential pathogens in the sample material varies the E BB Big 2 operator has to optimize pathogen inactivation by the Lysis Buffer or take appropriate measures E e ome EN SE i according to loca
58. s Aspirate the medium and wash cells with PBS not provided Aspirate the PBS and add 0 10 0 2596 trypsin After cells have detached from the dish or flask collect them in medium and transfer the appropriate number of cells up to 5 x 10 cells to a 1 5 ml microcentrifuge tube not provided Centrifuge for 5 min at 300 x g Remove the supernatant completely and discard taking care not to disturb the cell pellet Continue with MagCore Operation step 1 Using a cell scraper Detach cells from the dish or flask Transfer the appropriate number of cells up to 5 x 10 cells to a 1 5 ml microcentrifuge tube and centrifuge for 5 min at 300 x g Remove the supernatant completely and discard taking care not to disturb the cell pellet Continue with MagCore Operation step 1 MagCore Operation 1 Resuspend cell pellet with PBS Buffer to a final volume of 200 ul 2 Transfer cell mixture 200 uland add 20 ul Proteinase K into the MagCore Sample Tubes 3 Place the prepared Sample Tube into well 4 of T rack 4 Put the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 5 RunCode 110 program at MagCore Amniotic Fluid Protocol Sample preparation 1 Harvest cells from 10 15 ml amniotic fluid of 16 18 weeks by centrifugation for 10 minutes at 3000 rpm and discard the supernatant 2 Add 200yul GT Buffer not provided to the tube and resuspend the cell pellet then transfer mixture to new microcentrifu
59. st mnt aerei rais 36 sets Pipet Tip plus Holder Set eamenedetiehbus nnam npe 100 sets Sample TED as acsi e onu DR ID un is re dius 36 pcs Sample Eo Ae 100 pcs PEON TUGE BRENNEN IR I naan 36 pcs TS UT 6 1g BT 2 CB RI I Tene ener 100 pcs Proteinase NU TIO sacre ciimatiusacian IR erc etium EU onini 2 pcs Proteinase KTM S 4 pcs PK Storage TT ce cc cea Dex Ead aui cee un ai ERES 2 pcs PKStorage B ffel SOOO eee Ee DR EUN nO eee eu RU 4 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Shelf life 12 months Cartridge Contents SSS sg E ae a SS A ae T m m m Q m c m 3 8 8 8 8 8 Z EJBIR g l 88 88 S s lt s S o 2 dg E 38 S 8 RR S BIB BP IE B S Sz 32 x x S eis vs e 8 8 lt amp s S a S amp 3 S va va N S S S S S amp a Nu 9IS9It tise Description MagCore Genomic DNA Whole Blood Kit is designed for purification of total DNA including genomic mitochondrial and viral DNA from whole blood plasma serum buffy coat by using MagCore auto extraction instrument The method uses pre filled cartridge contains chaotropic salt to lysis cells and degrade protein DNA will bind to cellulose coated magnetic beads After washing off the contaminants the purified DNA is eluted by low salt elution buffer Purified DNA of approximately 20 30 kb in length is suita
60. ted ae 1 pcs RNase Free VEOIORosusinisundoninuaniuseppi in pardo ppl Ree 1 pcs Proteinase Kt Igni RR PHAR EET P NE E TE IEE 2 pcs PK Storage BUll a acts Gban brides iungi aisi ene 2 pcs Cartridge Contents OSS gt e SS gt g gt ive gt m tA tA Tg ENSE ERDPililiii 8 18 S S A A S S a S Cp 3 SS SS s E eile ls else leis Ls 88 S ME a a E E S S E Eg w m Om S9 m Q Q e E at S 2 c ni 3 gc 3 c S T T 3 T T S m e S gt a Q Sg Sg HUN e ae S S Us ca ea N 8 8 e S e 8 S S Ed n N e e amp e e T S ii m m Description MagCore Viral Nucleic Acid Extraction kit is designed to extract viral DNA and RNA via MagCore auto extraction instrument With all the kit components of plastic consumables are DNase RNase Free pretreated and individual processing track for each loaded samples this system eliminates all possible cross contamination between samples Built in protocol with flexibility in sample source volumes both DNA and RNA virus can be extracted using the same kit in a fast and economical way Applications Using magnetic particle technology to purify viral nucleic acid from serum plasma or cell free body fluids The purified viral nucleic acid is suitable for highly sensitive and quantitative PCR MagCore Viral
61. uch as PCR real time PCR restriction enzyme digestion southern blotting etc EC www rbcbioscience com MagCore Running Time 175 min 2 hour heating Standard 1012 min 16 hour heating High Yield Preparation before using 1 Add 1 1 ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10 mg ml at 20 C For needle like FFPE tissue slices 1 Add 500 ul Sula Oil 20 ul PK and the FFPE tissue sample to the bottom of Heat Block well 1 of cartridge and then cover it up with the Thermostable cap Note If the tissue is too large to lyse the surface area over 300 mn cut it in 4 sections Please see important notes step 3 before adding in the heat block well 1 would be suggested Make sure the tissue is at the bottom of the well to avoid clipping it by Thermostable cap 2 Place the Elution Tube into well 1 and the Tip Plus Holder Set into well 2 and 3 of T Rack 3 Run Code 405 program at MagCore For glass slide samples 1 Dropseveral Sula Oil on the glass slide and scrape them from the slide carefully then put in the bottom of Heat Block well 1 2 Add 500 ul Sula Oil and 20 ul PK into Heat Block well 1 rinse remaining sample on the wall and blade then cover it up with the Thermostable cap 3 Place the Elution Tube into well 1 the Tip Plus Holder Set into well 2 and 3 of T Rack 4 Run Code 405 program at MagCore de
62. uffer 4 Add 20yl Proteinase K close the lid and mix for 10 sec Incubate at 70 C for 10 minutes to lyse the sample 5 If there are any insoluble residues in the tube transfer the supernatant to Filter Column and centrifuge at full speed for 5min to get clear tissue solution in the Collection Tube 6 Pipette 400ul of clear tissue solution to the MagCore Sample Tube 7 Place the prepared Sample Tube into well 4 of T rack 8 Putthe Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack 9 Run Code 401 program at MagCore EE www rbcbioscience com MagCore Cultured Yeast Protocol e Additional requirements Sorbitol Buffer Lyticase or Zymolase Microcentrifuge tube e Preparation of Sorbitol Buffer 1 2 M sorbitol 10mM CaCi2 0 1M Tris Cl pH 7 5 Sterilize by filtration and store at 4 C Sample preparation 1 Harvest 3ml yeast cells up to 5x10 cells by centrifugation at 5000 x g for 10 minutes Discard the supernatant and carefully remove any remaining media by aspiration 2 Resuspend the cell pellet in 600ul sorbitol buffer not provided Cell Lysis Add 200U Lyticase or Zymolase not provided Incubate at 30 C for 30 minutes Centrifuge the mixture for 10 min at 2 000 x g to harvest Spheroplast 2 Remove the supernatant and add 400ul of GT Buffer to the tube and vortex or pipette to resuspend the cell pellet 3 Incubate at 55 C for 90min until the sample has been completely
63. us Holder SOL soatie niepm tipps unt rine 100 sets 2 0 T ERRARE RR nN 36pcs Sa ERR NIRE 100 pcs 2 Add 1ml xylene or substitute to the tube and vortex vigorously for 10sec Then incubate at 60 C for 10min X 8 78 1 TUGE eee ee NI RR I omer Neen ne ee 36 pcs S 8 7e 1 BUT o BRI m 100 pcs GT Buffer 3O0ml e ssstetnentttttttsettsttt ttt ttt tssoe btts 1 pcs GT Buffer 30mll eite ttt testati tasttt ttt 2 pcs 3 Centri fug eatfull sp eed for 3min at room temperature Filter Column Set 36pcs Filter Column Set 100 pcs 4 Remove the supernatant carefully by pipetting then add 1ml ethanol 96 10096 to the pellet and mix by vortexing for 10sec Proteinase NET ccs seteassecscainiteselustretnredeassg toad tite wheonsdb atest 1 pcs Proteinase NUT TMO dances e dupidti nuun dnd ER idee UR dtp 2 pcs PK Storage Bufffer ttt 1 pcs PK Storage Bufffer ttt 2pcs 5 Centrifuge at full speed for 5min at room temperature Storage and Stability 6 Remove the supernatant carefully by pipetting then add again of 1ml ethanol 96 100 to the pellet and mix by vortexing 1 This kit should be stored at room temperature for 10sec to wash again 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Shelflife 12 months 7 Centrifuge at full speed for 5min Cart
64. ut the Elution Tube into well 1 of T Rack and the Tip Plus Holder Set into well 2 of T Rack RunCode 201 program at MagCore N ALN KR W NS m Introduction of each MagCore Nucleic Acid Extraction kit MagCore Viral Nucleic Acid Extraction Kit For extraction of viral DNA RNA from serum plasma and cell free body fluids Cartridge Code 202 Cat No MVN400 03 MVN 400 04 Kit Contents Checkthatthe following parts are included in addition to the main unit Cat No MVN400 03 Contents Predilled Cartridge ieageht osa metre 36 pcs Pipet Tip plis Holder SE dcc otes cht utu RA eR apu n UE 36 sets Sample Tube M 36 pcs Elton TE sissies ree aera een aie 36 pcs MITER TRINA IG ss easacutusasiutianResi end RUBENS I MEO OR RR ME 1 pcs RNase Free WALCT C 1 pcs Proteinase KIMO assins 1 pcs PR Storage BUNT e 1 pcs Storage and Stability 1 This kit should be stored at room temperature 2 Carrier RNA should be stored at 20 C when mixing with RNase Free Water 3 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 4 Shelf life 12 months Cat No MVN400 04 Contents Pre filled Cartridge Reagent tosses een 96 pcs Pipet Tip plusHolderSel ps svctitsisaincctetcctictencanssieeieueniwencine 100 sets Sample THBE assente iesus mum unn vente Se ds ud 100 pcs Elution TUDE a nisse atus nitent iban URUU lb dU h 100 pcs Carrier NAHE eus asser
65. ystem will display 0 qp rJ i i 1 CoS a J zm E Complete page after completion i Select Sample Volume meis i 2A D L An A c v c wv Complete Confirm that the icon in the status bar is in normal 2 3 i RER 5 6 status If the icon shows the Start key will be at inactive ES CANCER 519 tatus and the operation is disabled Please follow the disph ee ees CER CANTEN TEA statusa p pay 2 viewing window or feeding door to OEN portion to remove error to solve Refer to page 8forwaming prevent from poor extraction result in i marks system operation if there is no emergency Confirm your input Sample Volume fi rond Press Enter for next page Press ESC for back to Stand By page cam le Tube V Nea Sop arses eee eee f www rbcbioscience com Introduction of each MagCore Nucleic Acid Extraction kit ENTUM MagCore Genomic DNA Whole Blood Kit Speedy installation For purification of genomic DNA from human whole blood Cartridge Code 101 Cat No MGB400 01 MGB400 02 Kit Contents Checkthatthe following parts are included in addition to the main unit Cat No MGB400 01 Contents Cat No MGB400 02 Contents Pre filled Cartridge Feagahl s uestis Eta Etuis 36 pcs Pre filled Cartridge Feggehl suac seeds bct SEES 96 pcs Pipet Tip plus Holder ST gestam stt gere itn in 36 sets Pipet Tip plus Holder SOL sse tutte pasto etit n pm 100 sets Sample IUD ccs Ee ea

MagCore® Viral Nucleic Acid Extraction Kit

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MagCore&reg; Viral Nucleic Acid Extraction Kit

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MagCore® Viral Nucleic Acid Extraction Kit