EPC 800 USB - HEKA Elektronik Dr. Schulze GmbH
Contents
1. OUTPUT V command mV Averaging INPUT monitor pA 10 al Holding mv 0 z xi _ Relative Cm_ Tracking gt Lab Book G The positive and negative responses are unequal Consider changing the holding lewel automatic capacitance com pensations are fully controlled through the software One of the many powerful features of PATCHMASTER is that it enables automatic control of the amplifier through user defined protocols Detailed information regarding PATCHMASTER functionality can be found in the PATCHMASTER brochure and user s manual Relative Value blacking Events Duplicate Delete Move Annotation Annotation This protocol preceeds each series with AutoC Slow Repeat Status IF etc Result 3 EPCMASTER software HEKA s EPCMASTER software consists of a virtual front panel of the EPC 800 USB Although the program has no functions for data acquisition or analysis it is very useful in that it provides users the option of setting amplifier parameters from a software panel instead of manually using the front panel controls EPCMASTER is provided free of charge and should be used in conjunction with a custom data acquisi tion system i e IGOR Pro Labview pCLAMP etc The end result is a software controlled version of the amplifier that is extremely flexible with regard what particular data acquisition interface or software package it Is being used with This flexibility allows2. 48 3 x 14 5 cm 12 3 x 19 x 5 7 inch Dimensions Main Unit Weight Main Unit 11 4 kg 24 8 Ibs Power Supply Power requirements are 125 W The logic controlled power supply automatically switches the voltage range Operational range is from 90 130V or 210 250V at line frequencies of 50 or 60 Hz A shielded transformer minimizes noise pickup from the power line frequencies Ground Lines Signal ground GND is isolated from the chassis by a 10 Q resistor to avoid ground loops GND is accessible via a Banana plug on the front panel and also via a connector on the headstage A Chassis ground CHAS is accessible via a Banana plug on the front panel and is connected to the ground line of the power cord Dimensions D x W x H 90 x 16 9 x 14 3 mm 3 54 x 0 67 x 0 57 inch Three feedback resistors Gain ranges can be switched during the experiment 5 MOhm 2 pA current range 500 MOhm 20 nA current range 50 GOhm 200 pA current range Resistors low gain range medium gain range high gain range Bandwidth max medium amp low ranges 100 kHz high range 60 kHz measured with an open input 8 pole Bessel filter and 50 GQ resistor DC to 1 kHz lt 0 03 pA RMS DC to 3 kHz lt 0 08 pA RMS DC to 10 kHz lt 0 225 pA RMS The EPC 800 USB has a filter knob on the front panel with settings of 0 1 0 3 0 5 0 7 1 3 5 7 10 30 and 100 kHz It is an integrated filter comprised of two built in filters for the c
3. Use with more recent models such as the Digidata 1440A that are equipped with telegraphing Ei A inputs enable the EPC 800 USB to be used in a local telegraphing Telegraphs mode In this situation the filter gain and C Slow values are tele Filter 5 kHz aL Gain 5 graphed and displayed within Clampex cee ne ae Ra UM In local or local telegraphing modes the automatic C Fast and C Slow compensations features of the EPC 800 USB can be utilized These are performed manually through the front panel and the results clearly illustrated in the Membrane Test panel of Clampex Membrane Test Cm 005 11 fF Rim Brdr hago Ra PE S Membrane Test gt u a eal our of a faecal Stays _Bot Batch EE Cur 19 83 pF Rm 476 6 MQ Ra 5 3 MQ p Tau 106 5 us Min Eg 3 Membrane Test k OK l cor ama Cm 444 be IH Ra Wd r Tau 444 p Hold 10 1 pA t SS ay oe 7 2 pa ee Co a Slower Faster Pulse mV Slower Faster Pulse mV EELIEEETETITEI 10 EEEEEETETETEI jo s Holding m va Holding mv BKB 7 He BEG 7 H fo j fo al OUTPUT OUTPUT V command mV R V command mV a ee j 1 INPUT 5 INPUT 5 I monitor pA I monitor pA Relative Cm al Meine X Se Tracking Lab Book lf bres a Lab Book A o0 Slo 4 gt fa Sio 2 PATCHMASTER software HEKA s PATCHMASTER software fully supports the EPC 800 USB in both local and remote modes
4. users to easily add an EPC 800 USB to their current setups without the need to purchase or learn a new data acquisition package Sled Ff EpcMaster Meli FE EPC800 Remote Mel V membrane l membrane Gain LFVC V memb Recording Mode V Uo Casto C fast Ato Range C slow 20 10pF__ Auto _ De Rscomp or StimFilt Filter 5kHz In this example ERCMASTER is being used with a Digidata 1440A and Clampex software the EPC 800 USB is now consid ered to be operated in remote mode 4 Custom software The EPC 800 USB is flexible enough so that it can be integrated into customized experimental systems The amplifier USB commands are open source and users are free to write their own interfacing to the device HEKA provides a dynamic link library DLL which gives access to the amplifier and HEKA data acquisition interfaces The DLL can be used with most programming languages such as C Pascal Delphi and Visual Basic gt N gt Q O aon d s LLI gt EPC 800 Patch Clamp Amplifier Acquisition Modes Three operating modes are provided Voltage Clamp VC Current Clamp Bridge CC Bridge and Low Frequency Voltage Clamp LFVC MODE CC Bridge vc 100 Voltage Clamp VC mode recordings ideal for recordings from whole cell cell attached single chan nel loose patch or bilayer configurations The current clamp mode of the EPC 800 USB is called CC Bridge Bridge compensation in
5. Electrophysiology Electrochemistry gt EPC 800 USB Patch Clamp Amplifier RS RANGI COVP ROW sh Stats id R mR oie ox Dasa SIGNAL EXTERNAL EXTERNAL VOLTAGE CURRENT CLIPPING patch clamp GNO INPUT CC INPUT YC MONITOR MONITOR EPC 800 REMOTE AUTO Veorrser UVCHOLE rt pz If you believe your job is to discover the EPC 800 make new discoveries Patch clamp amplifier with manual or computer controlled operation via USB e Can be used with any data acquisition interface with compatible acquisition soft ware e Low noise headstage optimized for single channel amp whole cell recordings e True current clamp mode for fast AP recordings e Automatic or manual Capacitance compensation FECA HEKA provides the finest instruments today to achieve the needed progress of tomorrow EPC 800 Patch Clamp Amplifier RS COMP w Aak VHOLD IHOLD POWER AUTO AUTO p 2 a E R SERIES C SLOW RANGE pF t FAST ys RS RANGE COMP REMOTE AUTO VPorpset LFVCHoLD C SLOW R SERIES C FAST FAST IVMON CURRENT FILTER kHz CHAS SIGNAL EXTERNAL EXTERNAL VOLTAGE CURRENT CLIPPING patch clamp GND INPUT CC INPUT VC MONITOR MONITOR EPC 800 ty J For any researcher who desires manual user control through knobs and dials while at the same time longs for some degree of computer communication and automatic control HEKA is excited to release the EPC 800 USB This amplifier is truly a uni
6. aphing e Telegraphing outputs on the rear panel for Gain Filter Bandwidth Mode and C Slow Telegraphing features can be utilized by use with ANY AD DA interface having telegraphing inputs e Amplifier remains under manual control with use of the knobs and switches on the front panel e Ability to perform automatic adjustments of Vp Offset C Fast and C Slow compensations are still possible TELEGRAPHING OUTPUT GAIN BANDWIDTH MODE C SLOW Ww Remote Commands are sent and received through USB 2 0 communication The EPC 800 USB commands are open source and users are free to write their own interfacing to the device e HEKA offers a dynamic link library DLL providing direct access to the amplifier and HEKA data acquisition interfaces HEKA s PATCHMASTER software supports all amplifier functionality in remote mode Amplifier can also be used with HEKA s ERCMASTER software in combination with custom software or third party software with the appropriate interface Front panel knobs and switches of the amplifier are inactive with the exception of the LCD multi position switch gt EPC 800 Patch Clamp Amplifier Acquisition Software Options 1 Clampex software The EPC 800 USB can be used with pCLAMP Clampex software REE kHz Using pCLAMP requires the use of one of the series of Digidata rh aI interfaces Use with older models such as the 1200 require the i i Highpass Hz amplifier to be used in local mode
7. current clamp mode acts in a similar way as RS compensation does in voltage clamp mode It can be thought of as an enhanced current clamp mode that fully compensates the voltage drop via the series access resistance of the electrode RS In this mode the stimulus artifact that is typically generated when injecting current is fully eliminated The current clamp circuitry of the EPC 800 USB acts as a voltage follower thereby increasing the speed and stability of the circuit Recording and following rapid events such as fast action potentials AP with patch or intracellular electrodes is possible The Low Frequency Voltage Clamp LFVC mode is a modified current clamp mode that allows for the measurement of potential deflections such as action potentials or synaptic potentials while the average potential is kept constant at a value chosen by the user with the LFVC _ potentiometer The circuit thus works like a current clamp for fast signals and like a voltage clamp for low frequency signals To achieve this the measured membrane potential is low pass filtered and compared to the LFVC oi potential Current is injected into the cell to keep the membrane potential at the chosen LFVC potential There are five time constants 1 3 10 30 and 100 available for the LFVC mode that specifiy the speed of regulation The feedback speed is highly dependent on the Gain Range settings C Fast and C Slow Compensation Both C Fast and C Slow compen
8. hone Fax Web Site Email Phone Fax Web Site Email 49 0 63 25 95 53 0 49 0 63 25 95 53 50 http Awww heka com sales heka com support heka com 1 902 624 0606 1 902 624 0310 http Awww heka com nasales heka com support heka com 1516 882 1155 1516 467 3125 http Awww heka com ussales heka com support heka com
9. n signals are injected via a 10 pF capacitor in medium and low gain and via a 1 pF capacitor in high gain range R Series 0 1 MQ lt 200 MQ 1000 pF range 1 MQ lt gt 200 MQ 100 pF range 5 MQ lt gt 200 MQ 30 pF range Series Resistance Compensation Manual adjustment with range depending on cell capacitance Equivalent Time Constants 2us 10us 100 us Range 0 95 In current clamp mode RS Comp serves as Bridge Compen sation with a range of 0 120 Telegraphing Outputs BNC connections on rear panel Gain Filter Bandwidth Amplifier Mode C Slow gt 5 2 2 N gt oa Q e pa i 2 LLI lt YA Q oc V gt Electrophysiology HEKA Elektronik Dr Schulze GmbH Wiesenstra e 71 D 67466 Lambrecht Pfalz Germany HEKA Electronics Incorporated 47 Keddy Bridge Road R R 2 Mahone Bay NS BOJ 2E0 Canada HEKA Instruments Inc 2128 Bellmore Avenue Bellmore New York 11710 5606 USA General notice Product names used herein are for identification purposes only and may be trademarks of their respective owners HEKA disclaims any and all rights in those marks We reserve the right to effect technical changes as develop ment progresses Special ver sions are available on request Further technical data are pro vided by a detailed description which is available on request A guarantee of one year applies on all instruments Electrochemistry Phone Fax Web Site Email P
10. of operation on either Windows 7 2000 XP and VISTA or Mac OS X operating systems When using PATCHMASTER the amplifier can be combined with any one of the HEKA InstruTECH series of inter faces ITC 16 ITC 18 ITC 1600 LIH 1600 LIH 8 8 When configured in Local mode the amplifier operates manually with the front panel knobs and switches of the EPC 800 USB all active As controls are changed the same values that are displayed on the front panel LCD display are read and displayed in the PATCHMASTER amplifier window iolxl Monitor J Tuning Show All ENEN Se e Se VC Off off Test Pulse show both amp Amplitude Length ound O noise Voume 1003 La Cov vo con Auto Range Zero Tracking C fast Auto 2 93 us ETA C slow 23 00 pF jC Awo Reseries 5 5 MOhm Rs Comp T0ps C 70 StimFilt 2s Filter _3 kHz D Reset Za Amplitude Duration P 4000m 7 0100ms 22 Protocol Editor Setup_Epcs00 cand aborts if K series is bigg REPEAT swee Amplifier C s 0 000 low 1C Bie 5 CSETUP 6 _SEAL_ _2_JOI6 l0 x When in Remote mode the controls and knobs of the EPC 800 USB are inactive All amplifier settings including IF K series gt 8 0000r BREAK protocol ENDIF Sweep W EL REPEAT 8 Membrane Test gon Anal out 0 Stage Bath Patch Cell Wy Wt P Hi Wt rs Hold 124 9 pA Slower Faster Pulse mV 666 7 Hz
11. que hybrid patch clamp am plifier with its design and feature set primarily based upon the manu ally controlled EPC 8 The EPC 800 USB is the most flexible patch clamp amplifier HEKA has ever produced in that it is a stand alone amplifier that can be combined with any existing AD DA interface and its compatible acquisition software Improvements in comparison to the EPC 8 e Telegraphing outputs for Gain Bandwidth Mode amp C Slow e Automatic Vp Offset C Fast amp C Slow compensation with the simple push of a button e Improved current monitor filters e USB 2 0 communication for software control of all amplifier functions e low Frequency Voltage Clamp Mode LFVC e CC Bridge Mode e Improved RS Comp Ranges gt 5 2 N gt oa Q e s d 2 LLI Features Operating Modes of the EPC 800 USB The EPC 800 USB can be operated in three modes Local Local Telegraphing and Remote The decision of which mode to use not only depends on user preference of whether or not to have the functionality to turn knobs and switches but also on what data acquisition software and interface the EPC 800 USB is used with 1 Local e Manual control by use of the knobs and switches on the front panel e V p Offset C Fast and C Slow compensation can be performed manually or automatically with the push of a button e Amplifier can be used with ANY data acquisition interface and its compatible software 2 Local Telegr
12. sation routines can either be performed manually by turning the knobs or automatically by pressing the Auto buttons on the front panel Both C Fast and C Slow compensa tion can be applied in all three headstage gain ranges The C Fast range is O to 15 pF in all gain ranges C Slow has 30 100 and 1000 pF ranges C SLOW C FAST Multi parameter Display An LCD panel can display the following parameter pairs 1 V C Fast fast C Slow R Series RS Range Comp VP LFVC I Vag and Noise The displayed parameters can either be individually selected via a multi position switch or automatically set If the Auto display mode is activated the LCD panel will automatically display the value of the control as its modified by the user RS RANGE COMP REMOTE C SLOW R SERIES VP LFVC C FAST t FAST WV or Holding Potentials and VpOffset Ten turn potentiometers are available on the front panel for VP liop 50 nA or 500 pA and LFVC olp 200 mV VP automatically by pressing the Auto button orrset 200 MV Vior 500 mV orse CAN De adjusted either manually or set Technical Specifications Included Accessories EPC 800 USB Headstage EPC 800 USB Model Circuit EPC 800 USB Manual Pipette Holder 1 5 mm is standard other dia meters available upon request at no additional charge 3 meter USB 2 0 shielded cable 2 meter IEC type shielded USB Cable 1 Power Cord 1 D x W x H 31 1 x
13. urrent monitor signal Filter 1 is a 5 pole 10 to 100 kHz Bessel pre filter Filter 2 is a 4 pole tunable 20 kHz Bessel filter When the front panel knob is set to either 30 or 100 kHz filter 2 is bypassed and the signal is straight filter 1 All of the other filter knob settings are filter 1 filter 2 Noise low gain range 0 005 0 01 0 02 0 05 0 1 0 2 mV pA medium gain range 0 5 1 2 5 10 20 mV pA high gain range 50 100 200 500 1000 2000 mV pA Pipette Offset 200 mV automatic or manual adjustment Holding Commands Voltage Clamp 500 mV with front panel knob 1 V via external Stim Input Current Clamp Bridge Low CC Output Gain Range I 50 nA lax 100 nA available when switching from VC mode in low gain range CC Stim Scaling 10 pA High CC Output Gain Range lyon 500 pA lax 1 nA available when switching from VC mode in either medium or high gain ranges CC Stim Scaling 0 1 pA Low Frequency Voltage Clamp LFVC 200 mV tT of 1 3 10 30 or 100 Capacitance Compensation C Fast Compensation 0 15 pF 0 gt 8 us tau Automatic or manual compensation in all gain ranges C Slow Compensation Automatic or manual compensation in all gain ranges OFF 30 100 1000 pF switch 30 pF range 1 0 30 pF 100 pF range 1 0 lt 100 pF 1000 pF range 1 0 lt gt 1000 pF Injection Capacitors The C Fast compensation signal is injected via a 1 pF capacitor The C Slow compensatio
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