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G1329-90012 - Agilent Technologies

1. sampler Vial number output 7 CAN cable y gt gt lt t to previous module is i ee ee Remote RS232C J Relay contacts GPIB Figure 14 Cable Connections If the front cover is not installed the autosampler is in a not ready condition and operation is inhibited The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF Agilent 1200 Series Autosampler User Manual 43 3 Installing the Autosampler Installing the Thermostatted Autosampler Parts required Description Sampler and Thermostat Power cord for the other cables see below and Cable Overview on page 120 Control Software ChemStation EZChrom OL etc and or Control Module G1323B Preparations Locate bench space Provide power connection Unpack the Sampler and the Thermostat WARNING Module is partially energized when switched off as long as the power cord is plugged in Risk of stroke and other personal injury Repair work at the module can lead to personal injuries e g shock hazard when the module cover is opened and the instrument is connected to power gt Never perform any adjustment maintenance or repair of the module with the top cover removed and with the power cord plugged in gt The security lever at the power input socket pr
2. 124 Agilent 1200 Series Autosampler User Manual Remote Cables Identifying Cables 9 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1100 1200 Series modules The other end depends on the instrument to be connected to Agilent 1100 1200 to 3390 Integrators Connector 01046 60203 Pin 3390 Pin Agilent Signal Name Active 1100 1200 TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low a NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low Agilent 1200 Series Autosampler User Manual 125 9 Identifying Cables Agilent 1100 1200 to 3392 3 Integrators Connector01046 60206 Pin 3392 3 Pin Agilent Signal Name Active 1100 1200 TTL 3 1 White Digital ground NC 2 Brown Prepare run Low 11 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 1200 to 3394 Integrators Connector01046 60210 Pin 3394 Pin Agilent Signal Name Active 1100 1200 TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 15 s 3 3 Gray Start Low NC 4 Blue Shut down Low e
3. Description Part number RS 232 cable instrument to PC 9 to 9 pin female This cable 24542U has special pin out and is not compatible with connecting G1530 60600 printers and plotters RS 232 cable kit 9 to 9 pin female and one adapter 9 pin 34398A male 25 pin female Suited for instrument to PC Cable Printer Serial amp Parallel is a SUB D 9 pin female vs 5181 1529 Centronics connector on the other end NOT FOR FW UPDATE This kit contains a 9 pin female to 9 pin female Null Modem 34398A printer cable and one adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1200 Series Autosampler User Manual 135 9 Identifying Cables RS 232 Cables 136 Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 10 Appendix General Safety Information 138 Lithium Batteries Information 142 Radio Interference 143 Sound Emission 144 Agilent Technologies on Internet 145 ee Agilent Technologies 137 10 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technol
4. 11 Reinstall the capillaries Next Steps 12 On completion of this procedure Install the front cover 13 Select End in the maintenance function Change piston Agilent 1200 Series Autosampler User Manual 101 7 Maintenance Simple Repairs Gripper Arm When Defective gripper arm Tools required Straightened paper clip Parts required Partnumber Description 1 G1313 60010 Gripper assembly Preparations Select Start in the maintenance function ChangeGripper Turn off the power to the autosampler Remove the front cover 1 Identify the slit below the gripper motor and the gripper 2 Rotate the arm approximately 2 5cm 1 inch to the left arm release button and insert the straightened paper clip into the slit button oo gt TOOTO ES SL SLAS AXA AAN 102 Agilent 1200 Series Autosampler User Manual Maintenance 7 Simple Repairs 3 Rotate the gripper arm slowly from left to right and apply a gentle pressure to the paper clip The clip will engage on an internal catch and the rotation of the arm will be blocked 4 Hold the paper clip in place press the gripper release button and rotate the gripper arm to the right 6 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left A A Next Steps 7 On completion of this procedure Inst
5. Figure 12 Recommended Stack Configuration for a thermostatted ALS Rear View 40 Agilent 1200 Series Autosampler User Manual Installing the Autosampler 3 Installing the Autosampler Parts required Preparations Description Sampler Power cord for the other cables see below and Cable Overview on page 120 Control Software ChemStation EZChrom OL etc and or Control Module G1323B Locate bench space Provide power connection Unpack the Sampler Module is partially energized when switched off as long as the power cord is plugged in Risk of stroke and other personal injury Repair work at the module can lead to personal injuries e g shock hazard when the module cover is opened and the instrument is connected to power gt Never perform any adjustment maintenance or repair of the module with the top cover removed and with the power cord plugged in gt The security lever at the power input socket prevents that the module cover is taken off when line power is still connected Never plug the power line back in when cover is removed Personal injury To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not bend the safety flap away from its position or attempt to remove the safety cover see Figure 13 on page 42 gt Do not attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle Agilent 120
6. e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 144 Agilent 1200 Series Autosampler User Manual Appendix 10 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1200 Series modules for download Agilent 1200 Series Autosampler User Manual 145 Index Index A Agilent oninternet 145 air circulation 26 algae information 54 ambient operating temperature 27 ambient non operating temperature 27 analog cable 120 122 analytical head 14 autosampler accessory kit contents 112 113 auxiliary cable 120 134 battery safety information 142 BCD cable 120 130 bench space 26 26 bypass 11 bypassing the autosampler 58 C cable analog 120 122 auxiliary 120 134 BCD 120 130 CAN 133 connecting APG remote 38 40 connecting the ChemStation 38 40 connecting the power 38 40 connecting CAN 38 40 146 connecting GPIB 38 40 connecting LAN 38 40 external contact 132 external contacts 121 GPIB 121 LAN 121 133 remote 120 125 RS 232 121 135 cables overview 120 CAN cable 133 capillaries 48 choice of vials and caps 68 cleaning 79 condensation 26 D damaged packaging 34 34 delay volume 51 58 58 delay 51 delivery checklist 34 34 dimensions 27
7. Auxiliary 3390 2 3 integrators 3394 6 integrators Agilent 35900A A D converter General purpose spade lugs 3390 integrator 3392 3 integrators 3394 integrator 3396A Series integrator 3396 Series Il 3395A integrator see details in section Remote Cables on page 125 3396 Series III 3395B integrator HP 1050 modules HP 1046A FLD HP 1046A FLD Agilent 35900A A D converter HP 1040 diode array detector HP 1090 liquid chromatographs Signal distribution module 3396 integrator General purpose spade Lugs Agilent 1100 Series vacuum degasser 01040 60101 35900 60750 35900 60750 01046 60105 01046 60203 01046 60206 01046 60210 03394 60600 03396 61010 5061 3378 5061 3378 5061 3378 01046 60202 01046 60202 01046 60202 03396 60560 G1351 81600 G1322 61600 Agilent 1200 Series Autosampler User Manual Identifying Cables 9 Type Description Part Number CAN cables Agilent 1100 1200 module to module 0 5m Ig 5181 1516 Agilent 1100 1200 module to module 1m Ig 5181 1519 External Agilent 1100 1200 Series interface board to general G1103 61611 contacts purpose GPIB cable Agilent 1100 1200 module to ChemStation 1 m 10833A Agilent 1100 1200 module to ChemStation 2 m 10833B RS 232 cable Agilent 1100 1200 module to a computer 34398A This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter LAN cable Twisted pair cross over LAN cable shielded 3m long for 5023 020
8. C 77 104 F Non condensing Operating Altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the module Safety standards IEC CSA Installation Category II Pollution For indoor use only Research UL Degree 2 Use Only Not for use in Diagnostic Procedures Using the autosampler at high environmental temperatures may cause the rear panel to become hot gt Do not use the autosampler at environmental temperatures higher than 50 C 122 F Agilent 1200 Series Autosampler User Manual 27 2 Performance Specifications 28 Site Requirements and Specifications Table5 Performance Specifications Agilent 1200 Series Autosampler G1329A Valid when standard 100 pl metering head installed Type Pressure GLP features Communications Safety features Injection range Replicate injections Precision Minimum sample volume Carryover Sample viscosity range Replicate injections per vial Sample capacity Injection cycle time Specification Operating range 0 40 MPa 0 400 bar 0 5900 psi Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 100 pl in 0 1 pl increments Up to 15
9. against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl O gt gt 2COCly 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Agilent 1200 Series Autosampler User Manual Using the Autosampler 4 e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid e Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1200 Series Autosampler User Manual 55 4 Using the Autosampler Sample Trays Supported trays for the different Autosampler Table 11 Supported trays for the Autosampler G1329A G2260A Description Part Number Tray for 100 x 2 ml vials G1313 44510 Halftray for 15 x 6 ml vials G1313 44513 Halftray for 40 x 2 ml vials G1313 44512 Thermostattable Tray for 100 x 2 ml vials G1329 60011 Halftray for 15 x 6 ml vials for G2260A only G1313 44513 1 This tray is not recommended when using a thermostat Half Tray Combinations Half tr
10. described in Table 18 on page 85 Exchanging Internal Parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the autosampler from the stack removing the covers and disassembling the autosampler Safety Flap Flex Board It is strongly recommended that the exchange of the safety flap and flex board is done by Agilent trained service personnel Transport Assembly Parts The adjustment of the motors and the tension on the drive belts are important for correct operation of the transport assembly It is strongly recommended that exchange of drive belts and the gripper assembly is done by Agilent trained service personnel There are no other field replaceable parts in the transport assembly If any other component is defective flex board spindles plastic parts the complete unit must be exchanged 76 Agilent 1200 Series Autosampler User Manual Maintenance 7 Updating the Firmware The Agilent 1200 Series LC modules are fitted with FLASH EPROMS These EPROMS enable you to update the instrument firmware from the ChemStation PCMCIA card or through the RS232 interface The firmware update procedure is described in the on line user information Agilent 1200 Series Autosampler User Manual 77 7 Maintenance Warnings and Cautions WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to pers
11. draw speed 68 68 DRAW 68 E ejectspeed 68 68 EJECT 68 electrical connections descriptions of 19 electrostatic discharge ESD environment 24 26 errormessages 73 ESD electrostatic discharge strap 78 78 external contact cable 121 132 F failure 73 flow connections 48 frequency range 27 fuses 24 G GPIB cable 121 gripper fingers 16 gripper alignment 73 external vials 73 H half trays 56 humidity 27 l information on sound emission 144 injection valve 8 13 injection volume precision 68 injection volumes less than 2 ul 68 injection volumes 68 injection sequence 11 injection valve 15 installation Agilent 1200 Series Autosampler User Manual Index power cords 25 installing the autosampler sample trays 56 installing the sample tray 50 installing the thermostatted autosampler power cable and interface cable 46 preparation 45 installing the thermostatted autosampler interface cables 44 power cable 44 safety 41 44 tray cover and front cover 58 installing the tray cover front door 58 installing the autosampler flow connections 48 interface cables 41 safety 41 installing the autosampler 41 power cable 41 internet 145 introduction to the autosampler 8 L LAN cable 121 133 line frequency 27 line voltage 27 lithium batteries 142 low volume injections 68 low volume capillary kit 58 M mainpass 11 maintenance functions 73 84 metering device 13 68 metering
12. gripper assembly and metering device for easy access when doing maintenance Step Commands The step functions provide the possibility to execute each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair Agilent 1200 Series Autosampler User Manual 73 6 Troubleshooting and Diagnostics Overview of the Sampler s Indicators and Test Functions 74 Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 7 Maintenance Introduction into Maintenance and Repair 76 Simple Repairs 76 Exchanging Internal Parts 76 Safety Flap Flex Board 76 Transport Assembly Parts 76 Updating the Firmware 77 Warnings and Cautions 78 Using the ESD Strap 78 Cleaning the module 79 Overview of Main Repair Procedures 80 Early Maintenance Feedback EMF 82 EMF Counters 82 Using the EMF Counters 83 Maintenance Functions 84 Simple Repairs 85 Needle Assembly 86 Needle Seat Assembly 89 Stator Face 91 Rotor Seal 94 Metering Seal and Plunger 98 Gripper Arm 102 Interface Board 104 ee Agilent Technologies 75 7 Maintenance Introduction into Maintenance and Repair Simple Repairs The autosampler is designed for easy repair The most frequent repairs such as change and needle assembly change can be done from the front of the instrument with the instrument in place in the system stack These repairs are
13. 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel adapter G1329 43200 Tray for 15 x 6 ml vials x2 G1313 44513 Union loop extension 5022 2133 Seat extension capillary 500 pl G1313 87307 Seat extension capillary 1500 pl G1313 87308 Sampler Column capillary G2260 87300 Pump Sampler capillary G2260 87301 Agilent 1200 Series Autosampler User Manual 113 Parts and Materials for Maintenance Maintenance Kit G1313 68730 for G1329A 114 Table 26 Maintenance Kit for G1329A Item Description Part Number 1 Rotor seal Vespel 0100 1853 2 Needle assembly 100 pl G1313 87201 3 Needle seat assembly 0 17 mm 2 3 ul G1313 87101 Agilent 1200 Series Autosampler User Manual Parts and Materials for Maintenance Maintenance Kit G1313 68719 for G1329B Table 27 Maintenance Kit for G1329A Item Description Part Number 1 Rotor seal PEEK 0101 1416 2 Needle assembly 100 pl G1313 87201 3 Needle seat assembly 0 17 mm G1313 87101 4 Metering seal pack of 2 5063 6589 5 Finger caps pack of 15 5063 6506 Agilent 1200 Series Autosampler User Manual 115 Parts and Materials for Maintenance Multi Draw Kit G1313 6871 116 Table 28 Multi Draw Kit for G
14. 1200 Series Autosampler User Manual Maintenance 7 Simple Repairs The procedures described in this section can be done with the autosampler in place in the stack You will do some of these procedures on a more frequent basis Table 18 Simple Repair Procedures Procedure Typical Frequency Time Required Notes Exchanging the needle When needle shows indication of 15 minutes See Needle Assembly on page 86 assembly damage or blockage Exchanging the seat When the seat shows indication of 10 minutes See Needle Seat Assembly on page 89 assembly damage or blockage Exchanging the rotor After approximately 30000 to 30 minutes See Rotor Seal on page 94 seal 40000 injections or when the valve performance shows indication of leakage or wear Exchanging the When autosampler reproducibility 30 minutes See Metering Seal and Plunger on metering seal indicates seal wear page 98 Exchanging the gripper When the gripper armis defective 10 minutes See Gripper Arm on page 102 arm Agilent 1200 Series Autosampler User Manual 85 7 Maintenance Needle Assembly When Tools required Parts required Preparations When the needle is visibly damaged When the needle is blocked 4inch wrench supplied in accessory kit 2 5mm Hex key supplied in accessory kit A pair of pliers Part number G1313 87201 G1329 80001 G1313 87202 G2260 87201 M Description Needle assembly for G1313 8710
15. 5 Optimizing Performance Precise Injection Volume 68 Injection Volumes Less Than 2 pl When the injection valve switches to the BYPASS position the mobile phase in the sample loop is depressurized When the syringe begins drawing sample the pressureof the mobile phase is decreased further If the mobile phase is not degassed adequately small gas bubbles may form in the sample loop during the injection sequence When using injection volumes lt 2 ul these gas bubbles may affect the injection volume precision For best injection volume precision with injection volumes lt 2 ul use of the Agilent 1200 Series degasser is recommended to ensure the mobile phase is adequately degassed Also using the automated needle wash see Optimization for Lowest Carry over on page 62 between injections reduces carry over to a minimum further improving the injection volume precision Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may have an influence on the injection volume precision when using viscous samples If the draw speed is too high air bubbles may form in the sample plug affecting precision The default draw speed is 200 ul min for the autosampler and 1000 ul min for the preparative autosampler This speed is suitable for the majority of applications however when using viscous samples set the draw speed to lower speed for optimum results A DRAW statement in an inject
16. Name 1200 White 1 EXT 1 a Brown 2 EXT 1 Green 3 EXT 2 Bea Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 132 Agilent 1200 Series Autosampler User Manual Identifying Cables 9 CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent 1200 Series module s CAN or LAN connectors CAN Cables Agilent 1200 module to module 0 5 m 5181 1516 Agilent 1200 module to module 1 m 5181 1519 Agilent 1200 module to control module G1323 81600 LAN Cables Description Part number Cross over network cable shielded 3 m long for point to 5023 0203 point connection Twisted pair network cable shielded 7 m long for hub 5023 0202 connections Agilent 1200 Series Autosampler User Manual 133 9 Identifying Cables Auxiliary Cable One end of this cable provides a modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Agilent 1100 Series Degasser to general purposes Connector G1322 81600 Color Pin Agilent Signal Name 1100 ee White 1 Ground n Brown 2 Pressure signal Green 3 Yellow 4 Grey 5 DC 5VIN Pink 6 Vent 134 Agilent 1200 Series Autosampler User Manual Identifying Cables 9 RS 232 Cables
17. Power supply 2 ASM board 3 Transport assembly 4 Metering seal Metering Seal and Plunger on page 98 5 Needle Needle Assembly on page 86 6 Rotor seal Rotor Seal on page 94 7 Needle seat Needle Seat Assembly on page 89 Agilent 1200 Series Autosampler User Manual 81 7 Maintenance Early Maintenance Feedback EMF Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user setable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The autosampler provides two EMF counters Each counter increments with autosampler use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The autosampler provides the following EMF counters Injection valve counter This counter display the total number of switches of the injection valve since the last reset of the counter Needle Movements Counter This counter di
18. may contribute to carry over in some instances When using small injection volumes or when injecting samples of low concentration immediately after samples of high concentration carry over may become noticeable Using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat 62 Agilent 1200 Series Autosampler User Manual Optimizing Performance 5 Using the Automated Needle Wash The automated needle wash can be programmed either as injection with needle wash or the needle wash can be included into the injector program When the automated needle wash is used the needle is moved into a wash vial after the sample is drawn By washing the needle after drawing a sample the sample is removed from the surface of the needle immediately Uncapped Wash Vial For best results the wash vial should contain solvent in which the sample components are soluble and the vial should not be capped If the wash vial is capped small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Injector Program with Needle Wash The injector program includes the command NEEDLE WASH When this command is included in the injector program the needle is lowered once into the specified wash vial before injection For example 1 DRAW 5 ul 2 NEEDLE WASH vial 7 3 INJECT Line 1 draws 5 ul from the current sample vial Line 2 moves the needle to
19. nl motor step 60 nl motor step Maximum stroke 100 ul 900 pl 900 pl Pressure limit 600 bar 200 bar 400 bar Plunger material Sapphire Sapphire Sapphire Agilent 1200 Series Autosampler User Manual Introduction to the Autosampler 1 Injection Valve The two position 6 port injection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass end positions No valve adjustments are required after replacing internal components Table2 Injection Valve Technical Data Standard Preparative MBB Autosampler SL Motor type 4V 1 2Asteppermotor 4V 1 2Asteppermotor 4V 1 2 A stepper motor Seal material Vespel Tefzel PEEK PEEK available Stator material Ceramic PEEK PEEK None Number of ports 6 6 6 Switching time lt 150 ms lt 150 ms lt 150 ms Agilent 1200 Series Autosampler User Manual 15 1 Introduction to the Autosampler Transport Assembly Transport Assembly The transport unit comprises an X axis slide left right motion a Z axis arm up down motion and a gripper assembly rotation and vial gripping X motor Theta motor Gripper motor Gripper Theta axis Z motor not in view Flex board Figure 7 Transport Assembly The transport assembly uses four stepper motors driven in closed loop mode for acc
20. o NC 5 Pink Not connected 16 zi NC 6 Yellow Power on High O 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected 126 Agilent 1200 Series Autosampler User Manual Identifying Cables 9 START and STOP are connected via diodes to pin 3 of the 3394 connector Agilent 1100 1200 to 3396A Integrators Connector03394 60600 Pin 3394 Pin Agilent Signal Name Active 1100 1200 TTL es 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low bs NC 4 Blue Shut down Low ae o NC 5 Pink Not connected le NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 1200 to 3396 Series II 3395A Integrators Use the cable part number 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1200 Series Autosampler User Manual 127 9 Identifying Cables Agilent 1100 1200 to 3396 Series III 3395B Integrators Connector03396 61010 Pin 33XX Pin Agilent Signal Name Active 1100 1200 TTL _ 9 1 White Digital ground NC 2 Brown Prepare run Low 8 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected 1 NC 6 Yellow Power on High O 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not conn
21. seal 900 ul 110 110 metering seal 108 missing parts 34 multi draw option 8 needle drive 13 14 non operating altitude 27 non operating temperature 27 numbering of vials 56 0 operating Altitude 27 operating temperature 27 optimizing performance automated needle wash 68 delay volume 68 delay volume adjustment 68 inject valve seal 68 low volume capillary kit 68 maintenance 68 optimizing performance bypassing the autosampler 58 low volume capillary kit 58 minimizing delay volume 51 58 58 P park transport assembly 34 park transport assembly 58 parts and materials 900 ul injection upgrade kit 117 accessory kit 112 external tray 118 main assemblies 106 maintenance kit 114 multi draw kit 116 preparative ALS acc kit 113 standard autosampler accessory kit 112 vial trays and tray base 111 parts and materials analytical head assembly optional 900 microlitre 108 analytical head assembly 108 Agilent 1200 Series Autosampler User Manual autosampler main assemblies 107 preparative head assembly 110 performance specifications autosampler 28 29 30 preparative autosampler 31 physical specifications 34 physical specifications 27 Power Consideration 24 power consumption 27 power cords 25 power requirements 24 preparative autosampler accessory kit contents 36 preparative head 14 radio interference 143 remote cable 120 125 repair procedures 80 repairs metering plunger 98 metering seal 98 needle
22. that any sample residue is removed from the metering unit and needle from before the next sampling sequence begins Different valves are available for the standard and preparative autosamplers Control of the vial temperature in the thermostatted autosampler is achieved using an additional Agilent 1200 Series module the ALS thermostat Details of this module are given in the Agilent 1200 Series thermostatted autosampler Supplemental Manual Figure 1 Overview of the Autosampler Agilent 1200 Series Autosampler User Manual 9 1 Introduction to the Autosampler Sampling Sequence 10 The movements of the autosampler components during the sampling sequence are monitored continuously by the autosampler processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence can t be completed successfully an error message is generated Solvent is bypassed from the autosamplers by the injection valve during the sampling sequence The sample vial is selected by a gripper arm from a static sample rack or from external vial positions The gripper arm places the sample vial below the injection needle The required volume of sample is drawn into the sample loop by the metering device Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The inje
23. to 5000 pl with multiple draw hardware modification required 1 99 from one vial Typically lt 0 5 RSD of peak areas from 5 2000 ul Typically lt 1 RSD of peak areas from 2000 5000 pl Typically lt 3 RSD of peak areas from 1 5 pl 1 ul from 5 pl sample in 100 pl microvial or 1 pl from 10 pl sample in 300 pl microvial 0 2 50 cp 100 x 2 ml vials in 1 tray 15 x 6 ml vials in tray Agilent vials only Typically 50 s depending on draw speed and injection volume Agilent 1200 Series Autosampler User Manual 31 2 Site Requirements and Specifications Performance Specifications 32 Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 3 Installing the Autosampler Unpacking the Autosampler 34 Damaged Packaging 34 Delivery Checklist 34 Optimizing the Stack Configuration 37 Installing the Autosampler 41 Installing the Thermostatted Autosampler 44 Flow Connections 48 Installing the Sample Tray 50 Transporting the Autosampler 51 ee Agilent Technologies 33 3 Installing the Autosampler Unpacking the Autosampler CAUTION Mechanical damage of the autosampler If the transport assembly is not parked the autosampler could be damaged due to excessive shock of the shipping container during transport gt Always park the transport assembly before shipment see Transporting the Autosampler on page 51 Damaged Packaging Upon receipt of your
24. uses an X Z Theta movement to optimize vial pick up and return Vials are picked up by the gripper arm and positioned below the sampling unit The gripper transport mechanism and sampling unit are driven by motors Movement is monitored by optical sensors and optical encoders to ensure correct operation The metering device is always flushed after injection to ensure minimum carry over The standard analytical head device provides injection volumes from 0 1 100 ul Two preparative head devices provide injection volumes from 0 1 900 ul One head is limited by a system pressure of 200 bars the other by a system pressure of 400 bars The G1329B autosampler SL uses an analytical head providing injection volumes from 0 1 100 ul for pressures up to 600 bar as used in rapid resolution systems The six port injection valve unit only 5 ports are used is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosamplers and directly connects the flow from the pump to Agilent 1200 Series Autosampler User Manual Analytical head Sampling Unit Transport assembly ASM board Power supply Vial Tray Gripper Arm Needle seat Switching Valve Introduction to the Autosampler 1 Introduction to the Autosampler the column During injection and analysis the valve unit directs the flow through the autosamplers which ensures that the sample is injected completely into the column and
25. 0 Series Autosampler User Manual 41 3 Installing the Autosampler CAUTION Defective on arrival problems If there are signs of damage to the autosampler please do not attempt to install the autosampler Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1 Install the LAN interface board in the sampler if required Safety cover Safety flap Figure 13 Safety Flap 2 Remove the adhesive tape which covers the front door Remove the front door and remove the transport protection foam A Place the Autosampler on the bench or in the stack as recommended in Optimizing the Stack Configuration on page 37 Ensure the power switch at the front of the Autosampler is OFF Connect the power cable to the power connector at the rear of the sampler Connect the CAN cable to the other Agilent 1200 Series modules on oo a If an Agilent Chemstation is the controller connect either The GPIB cable to the detector 42 Agilent 1200 Series Autosampler User Manual Installing the Autosampler 3 The LAN connector to the LAN interface 9 Connect the APG remote cable optional for non Agilent 1200 Series instruments 10 Turn ON power by pushing the button at the lower left hand side of the
26. 00 pl with multiple draw hardware modification required 1 99 from one vial lt 0 25 RSD from 5 100 ul lt 1 RSD 1 5 ul variable volume 1 ul from 5 pl sample in 100 pl microvial or 1 pl from 10 pl sample in 300 pl microvial Typically lt 0 1 lt 0 05 with external needle cleaning 0 2 50 cp 1 99 100 x 2 ml vials in 1 tray 40 x 2 ml vials in tray 15 x 6 ml vials in tray Agilent vials only Typically 50 s depending on draw speed and injection volume Agilent 1200 Series Autosampler User Manual Site Requirements and Specifications 2 Table6 Performance Specifications Agilent 1200 Series standard autosampler G1329A Valid when standard 900 ul metering head installed Type Specification Pressure Operating range 0 20 MPa 0 200 bar 0 2950 psi GLP features Early maintenance feedback EMF electronic records of Communications Safety features Injection range Replicate injections Precision Minimum sample volume Carryover Sample viscosity range Sample capacity Injection cycle time maintenance and errors Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 900 pl in 0 1 pl increments recommended 1 ul increments Up to 1800 pl with multiple draw hardware modif
27. 1 or G1313 87103 needle seat Needle assembly for G1329 87101 or G1329 87103 needle seat Needle assembly 900 ul loop cap for G1313 87101 needle seat Needle assembly 900 pl loop cap for G2260 87101 needle seat Select Start in the maintenance function Change Needle When the needle is positioned approx 15 mm above the needle seat remove the front cover Personal injury To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not bend the safety flap away from its position or attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle 86 Agilent 1200 Series Autosampler User Manual Maintenance 7 Simple Repairs 1 Select Needle Down until the needle screw is aligned 2 Remove the sample loop fitting from the needle fitting with the hole in the safety cover z i J A S Cat L 4 Insert the new needle Align the needle in the seat then tighten the screw firmly N LIN y Wr Agilent 1200 Series Autosampler User Manual 87 7 Maintenance Simple Repairs 5 Reconnect the sample loop fitting to the needle fitting 6 Use Needle Up to lift the needle to a position Next Steps 8 On completion of this procedure Install the front cover 9 Select End in the maintenance function Change Needle 88 Agilent 1200 Series Aut
28. 129 9 Identifying Cables BCD Cables a 000600000 A Oleaceovo One end of these cables provides a 15 pin BCD connector to be connected to the Agilent 1200 Series modules The other end depends on the instrument to be connected to Agilent 1200 to General Purpose Connector G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit 1200 Green 1 BCD 5 20 Violet 2 BCD 7 80 a Blue 3 BCD 6 40 S Yellow 4 BCD 4 10 oe Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 130 Agilent 1200 Series Autosampler User Manual Identifying Cables 9 Agilent 1200 to 3396 Integrators Connector03396 60560 Pin 3392 3 Pin Agilent Signal Name BCD Digit 1200 1 1 BCD 5 20 ae 2 2 BCD 7 80 K 3 3 BCD 6 40 4 4 BCD4 10 g 5 5 BCDO 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low Agilent 1200 Series Autosampler User Manual 131 9 Identifying Cables External Contact Cable 10 lt 6 8159F 9S 11 One end of this cable provides a 15 pin plug to be connected to Agilent 1200 Series module s interface board The other end is for general purpose Agilent 1200 Series Interface Board to general purposes Connector G1103 61611 Color Pin Agilent Signal
29. 1329A and G1329B Item Description Part Number 1 wo N N Seat capillary 500 pl 0 5 mm id Seat capillary 1500 pl 0 9 mm id Seat capillary 5000 pl Union G1313 87307 G1313 87308 0101 0301 5022 6515 Agilent 1200 Series Autosampler User Manual Parts and Materials for Maintenance 900 pl Injection Upgrade Kit G1363A for G1329A Table 29 900 pl Injection Upgrade Kit for G1329A only Item Description Part Number 1 Analytical Head 900 ul 2 Loop Extension 900 pl 3 Union loop extension 4 Needle 900 pl G1313 60007 G1313 87303 5022 2133 G1313 87202 Agilent 1200 Series Autosampler User Manual 117 8 Parts and Materials for Maintenance External Tray G1313 60004 Table 30 External Tray Item Description Part Number 1 External tray 2 Disposal tube G1313 60004 G1313 27302 118 Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 9 Identifying Cables Cable Overview 120 Analog Cables 122 Remote Cables 125 BCD Cables 130 External Contact Cable 132 CAN LAN Cables 133 Auxiliary Cable 134 RS 232 Cables 135 a Agilent Technologies 119 9 Identifying Cables Cable Overview 120 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Type Description Part Number Analog cables Remote cables BCD cables
30. 200 Series Autosampler User Manual 95 7 Maintenance 5 Install the new rotor seal and isolation seal Ensure the metal spring inside the isolation seal faces towards the valve body Install the stator ring with the short of the two pins facing towards you at the 12 O Clock position Ensure the ring sits flat on the valve body Install stator head and stator face Tighten the bolts yaar 5 LDG SS ENAN SS ZEN Sh 4 LE 3 alternately two turns at a time until the stator head is secure 96 Agilent 1200 Series Autosampler User Manual 7 Maintenance 10 Slide the waste tube into the waste holder in the leak tray os EEEN ee Pa e a i 9 Reconnect the pump capillaries to the valve ports see Figure 18 on page 49 11 On completion of this procedure Install the front cover 97 Agilent 1200 Series Autosampler User Manual 7 Maintenance Metering Seal and Plunger When Poor injection volume reproducibility Leaking metering device Tools required 1 4 inch wrench supplied in accessory kit 4 mm hex key supplied in accessory kit 3 mm hex key supplied in accessory kit Parts required Part number Description 1 5063 6589 Metering seal pack of 2 for 100 pl analytical head 1 Metering seal pack of 1 for 900 pl analytical head 1 5063 6586 Metering plunger for 100 pl analytical head 1 5062 8587 Metering plunger for
31. 3 point to point connection Twisted pair cross over LAN cable shielded 7m long for 5023 0202 point to point connection Agilent 1200 Series Autosampler User Manual 121 9 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent 1100 1200 Series modules The other end depends on the instrument to which connection is being made Agilent 1100 1200 to 3390 2 3 Integrators Connector 01040 60101 Pin 3390 2 3 Pin Agilent Signal Name 1100 1200 1 Shield Ground 2 Not connected 8 7 sepa 3 Center Signal 6 7 REN 4 Connected to pin 6 Al hie 5 Shield Analog 6 Connected to pin 4 7 Key 8 Not connected 122 Agilent 1200 Series Autosampler User Manual Identifying Cables 9 Agilent 1100 1200 to 3394 6 Integrators Connector35900 60750 Pin 3394 6 Pin Agilent Signal Name 1100 1200 1 Not connected 2 Shield Analog 3 Center Analog GIN 000 Agilent 1100 1200 to BNC Connector Connector8120 1840 Pin BNC Pin Agilent Signal Name 1100 1200 leq aS Shield Shield Analog JO Center Center Analog et Agilent 1200 Series Autosampler User Manual 123 9 Identifying Cables Agilent 1100 1200 to General Purpose Connector01046 60105 Pin 3394 6 Pin Agilent Signal Name 1100 1200 1 Not connected 2 Black Analog a 3 Red Analog
32. 5182 0543 5183 4492 5183 4494 write on spot Amber glass 2 5182 3376 5183 4493 5183 4495 write on spot Polypropylene 1 5182 0567 5183 4496 wide opening Polypropylene 0 3 9301 0978 wide opening Table 13 Snap Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0544 5183 4504 5183 4507 Clear glass 2 5182 0546 5183 4505 5183 4508 write on spot Amber glass 2 5182 0545 5183 4506 5183 4509 write on spot Agilent 1200 Series Autosampler User Manual Table 14 Screw Top Vials Using the Autosampler 4 Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0714 5183 2067 5183 2070 Clear glass 2 5182 0715 5183 2068 5183 2071 write on spot Amber glass 2 5182 0716 5183 2069 5183 2072 write on spot Table 15 Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE red rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE red rubber 5181 1217 Table 16 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber 5182 0550 Blue polypropylene Clear PTFE red rubber 5182 3458 Green polypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Clear PTFE red rubber 5182 3459 Agilent 1200 Series Autosampler User Manual 59 4 60 Using the Autosa
33. 60007 1 Plunger assembly 900 ul 5062 8587 2 Screws 0515 0850 3 Adapter 01078 23202 4 Support seal assembly 900 pl 5001 3764 5 Metering seal 900 pl 0905 1294 6 Head body 900 ul G1313 27700 7 Screw M5 60 mm lg for mounting of assembly not shown 0515 2118 here 1 This head is limited to 200 Bars Table 22 Preparative Head Assembly 900 pl for G2260A Item Description Part Number Preparative head assembly 900 ul includes items 1 6 G2260 60007 1 Plunger assembly 900 ul 5062 8587 2 Screws 0515 0850 3 Adapter 01078 23202 4 Support seal assembly 900 pl 5001 3764 5 Metering seal 900 ul 0905 1294 6 Head body 900 ul G2260 27700 7 Screw M5 60 mm lg for mounting of assembly not shown 0515 2118 here 1 This head is limited to 400 Bars It can only be assembled on a sampling unit with the description supports 900 ul at 400 Bar Agilent 1200 Series Autosampler User Manual Parts and Materials for Maintenance 8 Vial Trays Table 23 Thermostatted Autosampler Vial Trays and Tray Base Item Description Part Number 1 Adapter air channel G1329 43200 2 Tray for 100 x 2 ml vials thermostattable G1329 60011 3 Spring G1313 09101 4 Tray base for G1329A G1329B G2260A includes items 4 5 G1329 60000 5 Spring stud 0570 1574 Halftray for 40 x 2 ml vials not shown G1313 44512 Halftray for 15 x 6 ml vials not shown G1313 44513 1 amp S RNA WSJ Figure 25 Thermostatted Aut
34. 900 pl analytical head only if scratched or contaminated Preparations Select Start in the maintenance function Change piston Remove the front cover assembly 1 Remove the two capillaries from the metering head 2 Remove the two fixing bolts and pull the head assembly away from the sampler Notice that the closed side of the metering head faces upwards 98 Agilent 1200 Series Autosampler User Manual Maintenance 7 3 Remove the two fixing bolts from the base of the metering head assembly 4 Disassemble the metering head assembly 5 Use a small screwdriver to carefully remove the seal Clean the chamber with lint free cloth Ensure all particular matter is removed 6 Install the new seal Press the seal firmly into position Agilent 1200 Series Autosampler User Manual 99 7 Maintenance Y 7 Place the piston guide on top of the seal 8 Reassemble the metering head assembly Carefully insert the plunger into the base The closed side of the metering head must be on the same side as the lower one of the two capillary drillings 9 Install the fixing bolts Tighten the bolts securely 10 Install the metering head assembly in the autosampler Ensure the large hole in the metering head is facing downwards lt gt 2 E 100 Agilent 1200 Series Autosampler User Manual Maintenance 7 Simple Repairs
35. Agilent 1200 Series Standard and Preparative Autosamplers User Manual ot Agilent Technologies Notices Agilent Technologies Inc 2006 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61329 90012 Edition 11 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms th
36. Kit G1313 68719 for G1329B 115 Multi Draw Kit G1313 6871 116 900 ul Injection Upgrade Kit G1363A for G1329A 117 External Tray G1313 60004 118 ee Agilent Technologies 105 a SENA Q Uy SN Parts and Materials for Maintenance Main Assemblies Main Assemblies 10 oO s LO OY i Autosampler Main Assemblies Figure 23 Agilent 1200 Series Autosampler User Manual 106 Parts and Materials for Maintenance Table 19 Autosampler Main Assemblies Item Description Part Number 1 2 Transport assembly for G1329A 2260A Sampling unit assembly for G1329A Sampling unit assembly for G2260A The assy comes without injection valve and analytical head Analytical head assembly 100 pl for G1329A and G1329B Preparative head assembly 900 pl for G1329A P lt 200Bar Preparative head assembly 900 pl for G2260A P lt 400Bar Injection valve assembly for G1329A Injection valve assembly for G1329B Injection valve assembly for G2260A Vial tray thermostatted see Vial Trays on page 111 Gripper assembly Illumination assembly Autosampler Main Board ASM for G1329A and 2260A Autosampler Main Board ASM for G1329B Standoff GPIB connector part not shown Standoff remote connector part not shown Ribbon cable sample transport Ribbon cable sampling unit Sampler TCC cap 380 mm 0 1 mm id for G1329A Sampler Column cap 600 mm 0 5 mm id for G2260A Power supply
37. Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1200 Series Autosampler User Manual 25 2 Site Requirements and Specifications Bench Space The autosampler dimensions and weight see Table 4 on page 27 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches at the rear for the circulation of air and room for electrical connections Ensure the autosampler is installed in a horizontal position The thermostatted autosampler dimensions and weight allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 25 cm 10 inches of space on either side for the circulation of air and approximately 8 cm 3 1 inches at the rear for electrical connections Ensure the autosampler is installed in a level position If a complete Agilent 1200 Series system is to be installed on the bench make sure that the bench is designed to carry the w
38. No 2x 1x No Yes Interface board Yes Yes Yes Yes No No 1 The vacuum degasser will have a special connector for specific use For details see the degasser manual 2 The interface board slot not common to all modules provides specific interfacing needs external con tacts BCD LAN and so on Agilent 1200 Series Autosampler User Manual 21 1 22 Introduction to the Autosampler Agilent 1200 Series Interfaces Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 2 Site Requirements and Specifications Site Requirements 24 Power Consideration 24 Power Cords 25 Bench Space 26 Environment 26 Physical Specifications 27 Performance Specifications 28 ee Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimum performance of the instrument Power Consideration The autosampler power supply has wide ranging capability see Table 4 on page 27 Consequently there is no voltage selector in the rear of the autosampler There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply The thermostatted autosampler comprises two modules the standard or preparative autosampler and the thermostat G1330B Both modules have a separate power supply and a power plug for the line connections The two modules are connected by a control cable and both ar
39. Series Autosampler User Manual Appendix 10 Safety Symbols Table 31 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions Agilent 1200 Series Autosampler User Manual 141 10 Appendix Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste dispo
40. all the front cover 8 Turn the power to the autosampler ON Agilent 1200 Series Autosampler User Manual 103 7 Maintenance Interface Board When Tools required Parts required CAUTION At installation or when defective Flat head screwdriver Description 1 Interface board Electronic boards are static sensitive and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 104 oF Ww N Switch OFF the autosampler at the main power switch Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the autosampler Install the interface board Secure the screws Reconnect the cables to the board connectors Screws Figure 22 Exchanging the Interface Board Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 8 Parts and Materials for Maintenance Main Assemblies 106 Analytical Head Assembly 108 Vial Trays 111 Standard Autosampler Accessory Kit G1329 68725 112 Preparative Autosampler Accessory Kit G2260 68705 113 Maintenance Kit G1313 68730 for G1329A 114 Maintenance
41. ampler User Manual Stator Face Maintenance 7 When Poor injection volume reproducibility Leaking injection valve Tools required e 1 4 inch wrench supplied in accessory kit Hex key 9 64 inch supplied in accessory kit Parts required Part number Description 1 0100 1851 Stator face for G1329A 1 No stator face for G1329B functionality part of stator head 1 0101 1268 Stator face for G2260A Preparations Remove the front cover Remove the leak tubings if necessary Removing the stator head CAUTION The stator face is held in place by the stator head When you remove the stator head the stator face can fall out of the valve gt Carefully handle the valve to prevent damage to the stator face 1 Remove all capillary fittings from the injection valve ports 4 2 Loosen each fixing bolt two turns at a time Remove the bolts from the head ZA Done CD Vb SS s lt q DG 7 a Als VER any o gt et 7 Mobs SUNN CD WISER Agilent 1200 Series Autosampler User Manual 91 7 Maintenance 3 Remove the stator head and stator face 4 Place the stator face in place on the stator head Ensure the pins on the stator engage in the holes in the stator head 5 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure we N YN 6 Reconnect the pump capillaries to the valve ports s
42. assembly part not shown Screw M4 8 mm lg power supply part not shown BCD board not shown Cable autosampler to ALS thermostat part not shown G1329 60009 G1329 60008 G2260 60008 01078 60003 G1313 60007 G2260 60007 0101 0921 0101 1422 0101 1267 G1329 60011 G1313 60010 G1367 60040 G1329 69530 G1329 66540 0380 0643 1251 7788 G1313 81601 G1313 81602 01090 87306 G2260 87300 0950 2528 0515 0910 G1351 68701 G1330 81600 Agilent 1200 Series Autosampler User Manual 107 8 Parts and Materials for Maintenance Analytical Head Assembly Table 20 Analytical Head Assembly 100 pl for G1329A G1329B Item Description Part Number Analytical head assembly includes items 1 6 01078 60003 1 Plunger assembly 5063 6586 2 Screw M4 40 mm lg for mounting of assembly 0515 0850 3 Adapter 01078 23202 4 Support seal assembly 5001 3739 5 Metering seal pack of 2 5063 6589 6 Head body 01078 27710 7 Screw M5 60 mm lg for mounting of assembly not shown 0515 2118 here 108 Agilent 1200 Series Autosampler User Manual Parts and Materials for Maintenance 8 Analytical Head Assembly 7 not shown f Figure 24 Analytical Head Assembly Agilent 1200 Series Autosampler User Manual 109 110 Parts and Materials for Maintenance Table 21 Preparative Head Assembly 900 pl for G1329A only Item Description Part Number Analytical head assembly 900 ul includes items 1 6 G1313
43. assembly 86 needle seat assembly 89 rotor seal 94 simple repairs 85 stator face 91 using the ESD strap 78 78 RS 232 cable 121 RS 232C cable 135 S safety class 138 safety information lithium batteries 142 safety 147 Index general information 138 138 standards 27 symbols 141 sample trays 56 numbering of vial positions 57 sampling unit 13 sampling sequence 10 seals metering seal 108 110 110 shipping 34 58 simple repairs 85 site requirements 24 sound emission 144 specification physical 27 specifications 28 34 stack configuration 38 40 rearview 38 40 standard autosampler accessory kit contents 35 stator 15 status indicator 73 step functions 73 stepper motor 14 T temperature 28 theta axis 16 transport assembly 16 transport mechanism 8 transport 58 U unpacking the autosampler 34 34 V valve capillaries 48 vial contents temperature 28 148 vial numbering 56 vial racks 8 vial tray 58 vials 8 viscous samples 68 68 voltage range 27 volume 51 WwW weight 26 27 X X axis 16 Z Z axis 16 Agilent 1200 Series Autosampler User Manual www agilent com In This Book This manual contains user information about the Agilent 1200 Series Standard and Preparative Autosamplers The manual describes the following e introduction to the autosampler e site requirements and specifications e installing the autosampler e using the autosampler e optimizing performan
44. autosampler inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the autosampler has been checked mechanically and electrically If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection Delivery Checklist Ensure all parts and materials have been delivered with the autosampler The instrument box contains the instrument and an Accessory kit A separate box contains the reference manual and the power cable In Table 9 on page 35 and Table 10 on page 36 are listed the content of each accessory kit Please report missing or damaged parts to your local Agilent Technologies sales and service office 34 Agilent 1200 Series Autosampler User Manual Installing the Autosampler 3 Table9 G1329A G1329B Standard Autosampler Accessory Kit Contents G1329 68725 Description Part Number Tubing assembly 5063 6527 CAN cable 1 m long 5181 1519 Screw cap vials clear 100 pk 5182 0714 Blue screw caps 100 pk 5182 0717 Label halftray 5989 3890 Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 inch 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 Finger caps x3 5063 6506 Front door cooled au
45. ays can be installed in any combination enabling both 2 ml and 6 ml vials to be used simultaneously 56 Agilent 1200 Series Autosampler User Manual Using the Autosampler 4 Sample Trays Numbering of Vial Positions The standard 100 vial tray has vial positions 1 to 100 However when using two half trays the numbering convention is slightly different The vial positions of the right hand half tray begin at position 101 as follows Left hand 40 position tray 1 40 Left hand 15 position tray 1 15 Right hand 40 position tray 101 140 Right hand 15 position tray 101 115 Position 1 Left hand half tray Position 101 Right hand half tray Figure 20 Numbering of Tray Positions Agilent 1200 Series Autosampler User Manual 57 4 Using the Autosampler Choice of Vials and Caps 58 List of Compatible Vials and Caps For reliable operation vials used with the Agilent 1200 Series autosampler must not have tapered shoulders or caps that are wider than the body of the vial The vials in Table 12 on page 58 Table 13 on page 58 and Table 14 on page 59 and caps in Table 15 on page 59 Table 16 on page 59 and Table 17 on page 60 shown with their Part numbers have been successfully tested using a minimum of 15 000 injections with the Agilent 1200 Series autosampler Table 12 Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass 2
46. capabilities for all Agilent 1200 Series HPLC modules This includes tests and calibrations procedures as well as the different injector steps to perform all the maintenance routines Agilent Lab Advisor also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor Software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues Agilent 1200 Series Autosampler User Manual Troubleshooting and Diagnostics 6 Overview of the Sampler s Indicators and Test Functions Status Indicators The autosamplers are provided with two status indicators which indicate the operational state prerun run and error states of the instrument The status indicators provide a quick visual check of the operation of the autosampler Error Messages In the event of an electronic mechanical or hydraulic failure the instrument generates an error message in the user interface For details on error messages and error handling please refer to the Agilent Lab Monitor amp Diagnostic Software Maintenance Functions The maintenance functions position the needle arm
47. ce e troubleshooting and diagnostics e maintenance e parts and materials e cable overview e safety and warranty Agilent Technologies 2006 2008 Printed in Germany 11 08 G1329 90012 a Agilent Technologies
48. ce Feedback EMF 18 Electrical Connections 19 Agilent 1200 Series Interfaces 21 ee Agilent Technologies 1 Introduction to the Autosampler Introduction to the Autosampler Three models of Agilent 1200 Series autosamplers are available within this introduction they will be referred to as the standard autosampler G1329A the standard autosampler SL G1329B and the preparative autosampler G2260A Unless otherwise stated all information in this section is valid for all models The Agilent 1100 Series autosamplers and Agilent 1200 Series autosamplers are designed for use with other modules of the Agilent 1200 Series LC system with the HP 1050 Series or with other LC systems if adequate remote control inputs and outputs are available The autosamplerss are controlled from the Agilent 1200 Series control module G4208 A Instant Pilot or from the Agilent ChemStation for LC Three sample rack sizes are available for the autosamplers The standard full size rack holds 100 x 1 8 ml vials while the two half size racks provide space for 40 x 1 8 ml vials and 15 x 6 ml vials respectively Any two half size rack trays can be installed in the autosamplers simultaneously A specially designed sample rack holding 100 x 1 8 ml vials is available for use with thermostatted autosamplers The half size racks trays are not designed for an optimal heat transfer when they are used with a thermostatted autosampler The autosamplers transport mechanism
49. ction valve switches to the bypass position 2 The plunger of the metering device moves to the initialization position 3 The gripper arm moves from the home position and selects the vial At the same time the needle lifts out of the seat The gripper arm places the vial below the needle The needle lowers into the vial The metering device draws the defined sample volume The needle lifts out of the vial onion A If the automated needle wash is selected see Using the Automated Needle Wash on page 63 the gripper arm replaces the sample vial positions the wash vial below the needle lowers the needle into the vial then lifts the needle out of the wash vial 9 The gripper arm checks if the safety flap is in position 10 The gripper arm replaces the vial and returns to the home position Simultaneously the needle lowers into the seat 11 The injection valve switches to the mainpass position Agilent 1200 Series Autosampler User Manual Introduction to the Autosampler 1 Sampling Sequence Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position Figure 2 on page 11 In this position the mobile phase flows through the autosamplers metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over 1 Figure 2 Mainpass Position When the sample sequence begins t
50. di tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1200 Series Autosampler User Manual Contents Contents 1 Introduction to the Autosampler 7 Introduction to the Autosampler 8 Sampling Sequence 10 Sampling Unit 13 Transport Assembly 16 Early Maintenance Feedback EMF 18 Electrical Connections 19 Agilent 1200 Series Interfaces 21 2 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications 28 3 Installing the Autosampler 33 Unpacking the Autosampler 34 Optimizing the Stack Configuration 37 Installing the Autosampler 41 Installing the Thermostatted Autosampler 44 Flow Connections 48 Installing the Sample Tray 50 Transporting the Autosampler 51 4 Using the Autosampler 53 Solvent Information 54 Sample Trays 56 Choice of Vials andCaps 58 5 Optimizing Performance 61 Optimization for Lowest Carry over 62 Fast Injection Cycle and Low Delay Volume 66 Precise Injection Volume 68 Agilent 1200 Series Autosampler User Manual Contents Choice of Rotor Seal 70 Troubleshooting and Diagnostics 71 Agilent Lab Monitor amp Diagnostic Software 72 Overvi
51. e Autosampler on top of the Thermostat Make sure that the Autosampler is correctly engaged in the Thermostat locks Agilent 1200 Series Autosampler User Manual 45 3 46 Installing the Autosampler 6 Place the air channel adapter into the autosampler tray base Make sure the adapter is fully pressed down This assures that the cold airstream from the Thermostat is correctly guided to the tray area of the Autosampler Air channel adapter T Figure 16 Air channel adapter 7 Re install the tray 8 Ensure the power switch on the front of the Autosampler is OFF and the power cables are disconnected 9 Connect the cable between the Autosampler and the Thermostat see Figure 17 on page 47 10 Connect the power cables to the power connectors 11 Connect the CAN cable to the other Agilent 1200 Series modules 12 If an Agilent ChemStation is the controller connect either The GPIB cable to the detector The LAN connector to the LAN interface 13 Connect the APG remote cable optional for non Agilent 1200 Series instruments 14 Turn ON power by pushing the button at the lower left hand side of the sampler The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF Agilent 1200 Series Autosampler User Manual Installing the Autosampler 3 Installing the Thermostatted Autosampler Re
52. e stack in the position shown in Figure 9 on page 37 and Figure 10 on page 38 Figure 11 on page 39 and Figure 12 on page 40 show the configuration recommended for a thermostatted autosampler These configurations optimize the system flow path ensuring minimum delay volume Solvent cabinet Vacuum degasser Pump Instant Pilot Autosampler or Preparative Autosampler Column compartment Detector Figure 9 Recommended Stack Configuration for an Autosampler Front View Agilent 1200 Series Autosampler User Manual 37 3 Installing the Autosampler Optimizing the Stack Configuration Remote cable CAN Bus cable to Instant Pilot CAN Bus cable LAN to LC Analog signal to ChemStation recorder Figure 10 Recommended Stack Configuration for an Autosampler Rear View 38 Agilent 1200 Series Autosampler User Manual Installing the Autosampler Optimizing the Stack Configuration Detector Instant Pilot Column Compartment Solvent Standard or Cabinet Preparative 5 Autosampler egasser Pump ALS Thermostat Figure 11 Recommended Stack Configuration for a thermostatted ALS Front View Agilent 1200 Series Autosampler User Manual 39 3 Installing the Autosampler Optimizing the Stack Configuration LAN to LC ChemStation Analog signal to recorder Autosampler Thermostat cable AULA QUANIN Remote cable Analog signal to recorder CAN bus cable
53. e turned on by the autosampler module Damaged electronics Disconnecting or reconnecting the sampler to thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make sure the power cords are unplugged before disconnecting or reconnecting the sampler to thermostat cable Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage 24 Agilent 1200 Series Autosampler User Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear of the module The male end of each power cord is different and designed to match the wall socket of a particular country or region The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit Electric Shock gt
54. e warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated con
55. ected Agilent 1100 1200 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector5061 3378 Pin HP Pin Agilent Signal Name Active 1050 1100 1200 TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 128 Agilent 1200 Series Autosampler User Manual Agilent 1100 1200 to HP 1090 LC or Signal Distribution Module Identifying Cables 9 Connector01046 60202 Pin HP 1090 Pin Agilent Signal Name Active 1100 1200 TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 7 4 3 Gray Start Low E A a E 7 4 Blue Shut down Low si 8 5 Pink Not connected l NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 1200 to General Purpose Connector01046 60201 Pin Universal Pin Agilent Signal Name Active 1100 1200 TTL 1 White Digital ground i l 2 Brown Prepare run Low DE KEY 3 Gray Start Low m i a 4 Blue Shut down Low ss oo 5 Pink Not connected i 7 6 Yellow Power on High 7 Red Ready High 8 Green Stop Low 9 Black Start request Low Agilent 1200 Series Autosampler User Manual
56. ee Figure 18 on page 49 92 Agilent 1200 Series Autosampler User Manual Maintenance Simple Repairs 7 Slide the waste tube into the waste holder in the leak tray N Y a TI RY u 7 a d q lt Yj Y G A lt 2 Yi 8 On completion of this procedure Install the front cover Agilent 1200 Series Autosampler User Manual 93 7 Maintenance Rotor Seal When Poor injection volume reproducibility Leaking injection valve Tools required 1 4 inch wrench supplied in accessory kit e Hex key 9 64 inch supplied in accessory kit Parts required Part number Description 1 0100 1853 Rotor seal Vespel for G1329A 1 0100 1849 Rotor seal Tefzel for G1329A 1 0101 1416 Rotor seal PEEK for G1329B 1 0101 1268 Rotor seal PEEK for G2260A Preparations Remove front cover Remove the leak tubing if necessary CAUTION Removing the stator head The stator face is held in place by the stator head When you remove the stator head the stator face can fall out of the valve gt Carefully handle the valve to prevent damage to the stator face There is no stator face for G1329B 94 Agilent 1200 Series Autosampler User Manual Maintenance 7 1 Remove all capillary fittings from the injection valve ports 2 Loosen each fixing bolt two turns at a time Remove the bolts from the head Agilent 1
57. eight of all the modules For a complete system including the thermostatted autosampler it is recommended to position the modules in two stacks see Optimizing the Stack Configuration on page 37 Make sure that in this configuration there is 25 cm 10 inches space on either side of the thermostatted autosampler for the circulation of air Environment Your module will work within specifications at ambient temperatures and relative humidity as described in Table 4 on page 27 CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 26 Agilent 1200 Series Autosampler User Manual Site Requirements and Specifications 2 Physical Specifications Table 4 Physical Specifications Type Specification Comments Weight 14 2 kg 32 Ibs Dimensions 200 x 345 x 435 mm 8 x 13 5 x 17 width x depth x height inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 300 VA 200 W 683 BTU Maximum Ambient operating 0 55 C 32 131 F See warning on page 27 temperature Ambient non operating 40 70 C 4 158 F temperature Humidity lt 95 at 25 40
58. el seal is drastically reduced a seal made of Tefzel is available Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under normal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extreme mobile phase conditions PEEK Seal for preparative injection valve only The preparative injection valve has a sealing material made of PEEK This material has high chemical resistance and versatility It is suitable for application using mobile phases within a pH between 1 and 14 This seal is also used for the G1329B module Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible with PEEK Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual E ailitsseoutina and Diagnostics Agilent Lab Monitor amp Diagnostic Software 72 73 Overview of the Sampler s Indicators and Test Functions Agilent Technologies 71 6 Troubleshooting and Diagnostics Agilent Lab Monitor amp Diagnostic Software 72 The Agilent Lab Advisor Software is a standalone product that can be used with or without data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor provides diagnostic
59. events that the module cover is taken off when line power is still connected Never plug the power line back in when cover is removed WARNING Damaged electronics Disconnecting or reconnecting the autosampler to ALS thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make sure the power cords are unplugged before disconnecting or reconnecting the autosampler to ALS thermostat cable 44 Agilent 1200 Series Autosampler User Manual Installing the Autosampler 3 iim To avoid personal injury keep fingers away from the needle area during Autosampler operation gt Do not attempt to insert or remove a vial or a plate when the needle is positioned WARNING Damage through condensation If the condensation tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the instrument This may damage the instruments electronics gt Make sure that the condensation tube is always above the liquid level in the vessel 1 Place the Thermostat on the bench 2 Remove the front cover and route the condensation drain tube to the waste Condensation drain tube Waste bottle Figure 15 Condensation Leak outlet 3 Remove the adhesive tape which covers the front door 4 Remove the front door and remove the transport protection foam 5 Place th
60. ew of the Sampler s Indicators and Test Functions 73 Maintenance 75 Introduction into Maintenance and Repair 76 Overview of Main Repair Procedures 80 Early Maintenance Feedback EMF 82 Maintenance Functions 84 Simple Repairs 85 Parts and Materials for Maintenance 105 Main Assemblies 106 Analytical Head Assembly 108 Vial Trays 111 Standard Autosampler Accessory Kit G1329 68725 112 Preparative Autosampler Accessory Kit G2260 68705 113 Maintenance Kit G1313 68730 for G1329A 114 Maintenance Kit G1313 68719 forG1329B 115 Multi Draw Kit G1313 6871 116 900 pl Injection Upgrade Kit G1363A for G1329A 117 External Tray G1313 60004 118 Identifying Cables 119 Cable Overview 120 Analog Cables 122 Remote Cables 125 BCD Cables 130 External Contact Cable 132 CAN LAN Cables 133 Auxiliary Cable 134 RS 232 Cables 135 Agilent 1200 Series Autosampler User Manual Contents 10 Appendix 137 General Safety Information 138 Lithium Batteries Information 142 Radio Interference 143 Sound Emission 144 Agilent Technologies on Internet 145 Agilent 1200 Series Autosampler User Manual Contents 6 Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 1 Introduction to the Autosampler Introduction to the Autosampler 8 Sampling Sequence 10 Injection Sequence 11 Sampling Unit 13 Needle Drive 14 Analytical head preparative head 14 Injection Valve 15 Transport Assembly 16 Early Maintenan
61. he power input socket accepts a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 300 VA There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents the module cover from being taken off when line power is still connected Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1200 Series Autosampler User Manual 19 1 introduction to the Autosampler Electrical Connections Relay contacts Vial number output RS232C CAN bus Connection ALS Thermostat G1330B Thermostat optional Figure 8 Autosampler plus Thermostat Electrical Connections 20 Agilent 1200 Series Autosampler User Manual Introduction to the Autosampler 1 Agilent 1200 Series Interfaces The Agilent 1200 Series modules provide the following interfaces Table 3 Agilent 1200 Series Interfaces Interface Type Pumps Autosampler DA Detector VW Detector Thermostatted Vacuum MW Detector RI Detector Column Degasser FL Detector Compartment CAN Yes Yes Yes Yes Yes No GPIB Yes Yes Yes Yes Yes No RS 232C Yes Yes Yes Yes Yes No APG Remote Yes Yes Yes Yes Yes Yes Analog Yes
62. he valve unit switches to the bypass position Figure 3 on page 11 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 I Figure 3 Bypass Position Agilent 1200 Series Autosampler User Manual 11 1 Introduction to the Autosampler Sampling Sequence Next the needle is raised and the vial is positioned below the needle The needle moves down into the vial and the metering unit draws the sample into the sample loop Figure 4 on page 12 J Figure 4 Drawing the Sample When the metering unit has drawn the required volume of sample into the sample loop the needle is raised and the vial is replaced in the sample tray The needle is lowered into the needle seat and the injection valve switches back to the mainpass position flushing the sample onto the column Figure 5 on page 12 Figure 5 Mainpass Position Sample Injection 12 Agilent 1200 Series Autosampler User Manual Introduction to the Autosampler 1 Sampling Unit Sampling Unit The sampling unit comprises three main assemblies needle drive metering device and injection valve NOTE The replacement sampling unit excludes the injection valve and metering head assemblies The sampling units for the standard and the preparative autosamplers are different Needle drive Metering device Injection valve Figure 6 Autosampler Sampling Unit Agilent 1200 Series Autosample
63. ication required 1 99 from one vial Typically lt 0 5 RSD of peak areas from 5 2000 ul Typically lt 1 RSD of peak areas from 2000 5000 ul Typically lt 3 RSD of peak areas from 1 5 pl 1 ul from 5 pl sample in 100 pl microvial or 1 pl from 10 pl sample in 300 pl microvial Typically lt 0 1 lt 0 05 with external needle cleaning 0 2 50 cp 100 x 2 ml vials in 1 tray 40 x 2 ml vials in tray 15 x 6 ml vials in tray Agilent vials only 50 s for draw speed 200 pl min ejection speed 200 l min injection volume 5 ul Agilent 1200 Series Autosampler User Manual 29 Site Requirements and Specifications Table 7 Performance Specifications Agilent 1200 Series standard autosampler SL G1329B Type Specification Pressure Operating range 0 60 MPa 0 600 bar 0 8850 psi GLP features Early maintenance feedback EMF electronic records of Communications Safety features Injection range Replicate injections Precision Minimum sample volume Carryover Sample viscosity range Sample capacity Injection cycle time maintenance and errors Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 100 pl in 0 1 pl increments recommended 1 ul increments Up to 15 00 ul with m
64. lay contacts Vial number output Thermostat Autosampler cable 0000000 ooo0000 Remote RS232C CAN bus GPIB Figure 17 Cable Connections Agilent 1200 Series Autosampler User Manual 47 3 Installing the Autosampler Flow Connections Parts required Description 1 Parts from the Accessory kit Preparations Sampler is installed in the LC system WARNING Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Connect the pump outlet capillary to port 1 of the injection valve 2 Connect column compartment inlet capillary to port 6 of the injection valve Connect the corrugated waste tube to the solvent waste from the leak plane 4 Ensure that the waste tube is positioned inside the leak channel Do not extend the waste capillary of the autosampler The siphoning effect might empty the complete seat capillary introducing air into the system 48 Agilent 1200 Series Autosampler User Manual Pump outlet capillary Waste tube in Leak channel Solvent waste for corrugated waste tube Column compartment i
65. mpler Table 17 Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red polypropylene Clear PTFE silicone 5182 0722 Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 5 Optimizing Performance Optimization for Lowest Carry over 62 Using the Automated Needle Wash 63 Using an Injector Program 64 General Recommendation to Lowest Carry over 65 Fast Injection Cycle and Low Delay Volume 66 Overlapped Injection Mode 66 General Recommendations for Fast Injection Cycle Times 67 Precise Injection Volume 68 Draw and Eject Speed 68 Choice of Rotor Seal 70 ee Agilent Technologies 61 5 Optimizing Performance Optimization for Lowest Carry over Several parts of an injection system can contribute to carry over e needle outside e needle inside e needle seat e sample loop e seat capillary e injection valve The autosampler continuous flow through design ensures that sample loop needle inside seat capillary and the mainpass of the injection valve is always in the flow line These parts are continuously flushed during an isocratic and also during a gradient analysis The residual amount of sample remaining on the outside of the needle after injection
66. n increase the carry over in the system The injection cycle times also depend on the injection volume In identically standard condition injecting 100 ul instead of 1 ul increase the injection time by approximately 8 sec In this case and if the viscosity of the sample allows it the draw and eject speed of the injection system has to be increased Agilent 1200 Series Autosampler User Manual Optimizing Performance 5 For the last injection of the sequence with overlapped injections it has to be considered that for this run the injection valve is not switched as for the previous runs and consequently the injector delay volume is not bypassed This means the retention times are prolonged for the last run Especially at low flow rates this can lead to retention time changes which are too big for the actual calibration table To overcome this it is recommended to add an additional blank injection as last injection to the sequence General Recommendations for Fast Injection Cycle Times As described in this section the first step to provide short cycle times are optimizing the chromatographic conditions If this is done the autosampler parameter should be set to e Overlapped injection mode e Increase of draw and eject speed for large injection volumes e Add at last run a blank if overlapped injection is used To reduce the injection time the detector balance has to be set to OFF Agilent 1200 Series Autosampler User Manual 67
67. nlet capillary Figure 18 Hydraulic Connections Agilent 1200 Series Autosampler User Manual Installing the Autosampler Flow Connections 3 49 3 Installing the Autosampler Installing the Sample Tray 1 Open the front door 2 Load the sample tray with sample vials as required Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 4 Press the front of the sample tray down to secure the tray in the autosampler If the thermostatted autosampler tray pops out of position the air channel adapter is not inserted correctly Figure 19 Installing the Sample Tray 50 Agilent 1200 Series Autosampler User Manual Installing the Autosampler 3 Transporting the Autosampler When moving the autosampler around the laboratory no special precautions are needed However if the autosampler needs to be shipped to another location via carrier ensure e The transport assembly is parked e The vial tray is secured If the autosampler is to be shipped to another location the transport assembly must be moved to the park position to prevent mechanical damage should the shipping container be subjected to excessive shock Also ensure the vial tray is secured in place with suitable packaging otherwise the tray may become loose and damage internal components Agilent 1200 Series Autosampler User Manual 51 3 Installing the Autosampler Transp
68. ogies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 138 Agilent 1200 Series Autosampler User Manual Appendix 10 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in
69. onal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Using the ESD Strap Electronic boards are sensitive to electronic discharge ESD In order to prevent damage always use an ESD kit when handling electronic boards and components Use the ESD kit according to recommendations given by the supplier 78 Agilent 1200 Series Autosampler User Manual Maintenance 7 Cleaning the module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an exessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings Agilent 1200 Series Autosampler User Manual 79 Maintenance Overview of Main Repair Procedures 7 Overview of Main Repair Procedures f L Main Assemblies Figure 21 Agilent 1200 Series Autosampler User Manual Maintenance 7 erview of Main Repair Procedures 1
70. or program also uses the draw speed setting which is configured for the autosampler Agilent 1200 Series Autosampler User Manual Optimizing Performance 5 Eject Speed The default eject speed setting is 200 ul min for the standard autosampler and 1000 ul min for the preparative autosampler When using large injection volumes setting the eject speed to a higher value speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the plunger returns to the home position An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens the time required to run the injector program When using viscous samples a high eject speed should be avoided Agilent 1200 Series Autosampler User Manual 69 5 Optimizing Performance Choice of Rotor Seal 70 Vespel Seal for standard valves only The standard seal has sealing material made of Vespel Vespel is suitable for applications using mobile phases within the pH range of 2 3 to 9 5 which is suitable for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime Tefzel Seal for standard valve only For mobile phases with pH below 2 3 or above 9 5 or for conditions where the lifetime of the Vesp
71. orting the Autosampler 52 Agilent 1200 Series Autosampler User Manual Agilent 1200 Series Autosampler User Manual 4 Using the Autosampler Solvent Information 54 Sample Trays 56 Choice of Vials and Caps 58 ee Agilent Technologies 53 4 Using the Autosampler Solvent Information 54 Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blockage damage of the flow cell If the flow cell is transported while temperatures are below 5 C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive
72. osampler User Manual Maintenance Needle Seat Assembly When When the seat is visibly damaged When the seat capillary is blocked Tools required Parts required Preparations 1 4 inch wrench supplied in accessory kit Flat head screwdriver Part number G1313 87101 G1313 87103 G2260 87101 Description Needle seat assy 0 17 mm i d 2 3 pl for G1329A B Needle seat assy 0 12 mm i d 1 2 pl for G1329A B Needle seat assy 0 50 mm i d 20 pl for G2260A Select Start in the maintenance function Change Needle Remove the front cover Use the Needle Up command in the Change Needle function to lift the needle an addition 1 cm 1 Disconnect the seat capillary fitting from the injection valve port 5 seat 2 Usea small flat head screwdriver to ease out the needle Agilent 1200 Series Autosampler User Manual 7 Maintenance 3 Insert the new needle seat assembly Press the seat 4 Connect the seat capillary fitting to port 5 of the injection firmly into position valve 5 Use Down to position the needle approximately 2mm 6 Ensure the needle is aligned with the seat If required above the seat bend the needle slightly until the needle is aligned correctly Next Steps 7 On completion of this procedure Install the front cover 8 Select End in the maintenance function Change Needle 90 Agilent 1200 Series Autos
73. osampler Vial Trays and Tray Base Agilent 1200 Series Autosampler User Manual 111 Parts and Materials for Maintenance Standard Autosampler Accessory Kit G1329 68725 112 Table 24 G1329A G1329 B Standard Autosampler Accessory Kit Contents G1329 68725 Description Part Number Flexible tubing assy 120 cm 5063 6527 Filter promo kit no PN CAN cable 1 m long 5181 1519 Screw cap vials clear 100 pk 5182 0714 Blue screw caps 100 pk 5182 0717 Label halftray no PN Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel adapter G1329 43200 Cover insulation no PN Capillary 0 17 mm 900 mm G1329 87300 Capillary heat exchanger 01090 87306 Note for Agilent 1200 Series Autosampler door upgrade no PN Agilent 1200 Series Autosampler User Manual Parts and Materials for Maintenance 8 Preparative Autosampler Accessory Kit G2260 68705 Table 25 G2260A Preparative Autosampler Accessory Kit Contents G2260 68705 Description Part Number Flexible tubing assy 120 cm 5063 6527 Filter promo kit no PN CAN cable 1 m long 5181 1519 Screw cap vials clear 100 pk 5182 0714 Blue screw caps 100 pk 5182 0717 Label halftray no PN Wrenches
74. propriate solvent on to the needle seat The solvent should be able to dissolve the contamination If this is not known use 2 or 3 solvents of different polarity Use several milliliters to clean the seat e Clean the needle seat with a tissue and remove all liquid from it e RESET the injector Agilent 1200 Series Autosampler User Manual 65 5 Optimizing Performance Fast Injection Cycle and Low Delay Volume 66 Short injection cycle times for high sample througput is one of the most important requirements in analytical laboratories In order to shorten cycle times you can e shorten the column length e use high flow rates e apply a steep gradient Having optimized these parameters further reduction of cycle times can be obtained using the overlapped injection mode Overlapped Injection Mode In this process as soon as the sample has reached the column the injection valve is switched back to bypass and the next injection cycle starts but waits with switching to mainpass until the actual run is finished You gain the sample preparation time when using this process Switching the valve into the bypass position reduces the system delay volume the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory in narrow bore and micro bore HPLC Having the valve in bypass position ca
75. r User Manual 13 1 14 Introduction to the Autosampler Needle Drive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly The upper and lower needle positions are detected by reflection sensors on the sampling unit flex board while the needle in vial position is determined by counting the motor steps from the upper needle sensor position Analytical head preparative head The analytical head is driven by the stepper motor connected to the drive shaft by a toothed belt The drive nut on the spindle converts the circular movement of the spindle to linear motion The drive nut pushes the sapphire plunger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampling unit flex board while the sample volume is determined by counting the number of steps from the home position The backward movement of the plunger driven by the spring draws sample from the vial Table 1 Analytical Head Technical Data Standard 100 pl Standard 900 pl Preparative 900 pl Number of steps 15000 15000 15000 Volume resolution 7 nl motor step 60
76. sal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 142 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 1200 Series Autosampler User Manual Appendix 10 Radio Interference Cables supplied by Agilent Technoligies are screened to provide opitimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1200 Series Autosampler User Manual 143 10 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A
77. splays the total number of movements of the needle into the seat since the last reset of the counter 82 Agilent 1200 Series Autosampler User Manual Maintenance 7 Using the EMF Counters The user setable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of autosampler components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary make note of the values displayed by the injection valve and needle movements counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1200 Series Autosampler User Manual 83 7 Maintenance Maintenance Functions Certain maintenance procedures require the needle arm metering device and gripper assembly to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position 84 Agilent
78. the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Agilent 1200 Series Autosampler User Manual 139 10 Appendix When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 140 Agilent 1200
79. tosampler G1329 40301 Air channel adapter G1329 43200 Cover insulation no PN Capillary 0 17 mm 900 mm G1329 87300 Capillary heat exchanger 01090 87306 Note for Agilent 1200 Series Autosampler door upgrade no PN 1 Reorder gives pack of 15 Agilent 1200 Series Autosampler User Manual 35 3 36 Installing the Autosampler Table 10 G2260A Preparative Autosampler Accessory Kit Contents G2260 68705 Description Part Number Tubing assembly 5063 6527 Filter promo kit 5064 8240 CAN cable 1 m long 5181 1519 Screw cap vials clear 100 pk 5182 0714 Blue screw caps 100 pk 5182 0717 Label halftray 5989 3890 Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 inch 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler Air channel adapter Tray for 15 x 6 ml vials x2 Union loop extension Seat extension capillary 500 pl Seat extension capillary 1500 pl Sampler Column capillary G1329 40301 G1329 43200 G1313 44513 5022 2133 G1313 87307 G1313 87308 G2260 87300 Agilent 1200 Series Autosampler User Manual Installing the Autosampler 3 Optimizing the Stack Configuration Optimizing the Stack Configuration If your autosampler is part of a system you can ensure optimum performance by installing the autosampler in th
80. ts have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled For details on EMF counters and how to use them see Early Maintenance Feedback EMF on page 82 Agilent 1200 Series Autosampler User Manual Introduction to the Autosampler 1 Electrical Connections e The GPIB connector is used to connect the module with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your module The switches are preset to a default address and is recognized once after power is switched ON e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1200 Series module data transfer and synchronization e One analog output provides signals for integrators or data handling systems e The interface board slot is used for external contacts and BCD bottle number output or LAN connections e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch See your software documentation for further information e T
81. ultiple draw hardware modification required 1 99 from one vial Typically lt 0 25 RSD of peak areas from 5 100 ul Typically lt 1 RSD of peak areas from 1 5 ul 1 ul from 5 pl sample in 100 pl microvial or 1 pl from 10 pl sample in 300 pl microvial Typically lt 0 1 lt 0 05 with external needle cleaning 0 2 50 cp 100 x 2 ml vials in 1 tray 40 x 2 ml vials in tray 15 x 6 ml vials in tray Agilent vials only 50 s for draw speed 200 pl min ejection speed 200 l min injection volume 5 ul Agilent 1200 Series Autosampler User Manual Site Requirements and Specifications 2 Table8 Performance Specifications Agilent 1200 Series Preparative Autosampler G2260A Type Specification Pressure Operating range 0 40 MPa 0 400 bar 0 5800psi GLP features Early maintenance feedback EMF electronic records of Communications Safety features Injection range Replicate injections Precision Minimum sample volume Sample viscosity range Sample capacity Injection cycle time maintenance and errors Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 900 pl in 0 1 pl increments recommended 1 ul increments Up to 1800 pl with multiple draw hardware modification required Up
82. urate positioning of the gripper assembly for sample vial transport The rotational movement of the motors is converted to linear motion X and Z axes by toothed belts connected to the drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly 16 Agilent 1200 Series Autosampler User Manual Introduction to the Autosampler 1 The stepper motor positions are determined by the optical encoders mounted onto the stepper motor housing The encoders monitor the position of the motors continually and correct for position errors automatically e g if the gripper is accidentally moved out of position when loading vials into the vial tray The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly Agilent 1200 Series Autosampler User Manual 17 1 Introduction to the Autosampler Early Maintenance Feedback EMF 18 The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user setable limi
83. vial 7 Line 3 injects the sample valve switches to main pass Agilent 1200 Series Autosampler User Manual 63 5 64 Optimizing Performance Using an Injector Program The process is based on a program that switches the bypass grove of the injection valve into the flow line for cleaning This switching event is performed at the end of the equilibration time to ensure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are used For example Outside wash of needle in vial 7 before injection Injector program Draw x x y ul from sample NEEDLE WASH vial 7 Inject Wait equilibration time see text above Valve bypass Wait 0 2 min Valve mainpass Valve bypass Valve mainpass Overlapped injection together with additional injection valve switching is not possible Agilent 1200 Series Autosampler User Manual Optimizing Performance 5 General Recommendation to Lowest Carry over e For samples where needle outside cannot be cleaned sufficiently with water or alcohol use wash vials with an appropriate solvent Using an injector program and several wash vials can be used for cleaning In case the needle seat has got contaminated and carry over is significantly higher than expected the following procedure can be used to clean the needle seat e Go to MORE INJECTOR and set needle to home position e Pipette an ap

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