Leica DM750 User Manual
Contents
1. Time Delay Shutoff The Leica DM750 is equipped with a time Changing the status of the time delay e The LED Illumination will flash to indicate delay shutoff capability which automati shutoff the time delay shutoff status was changed cally turns the illumination off after 2 hours of 1 1 Rotate the intensity regulator to the lowest o no changes in the intensity control level e The LED Illumination will flash two times slowly then stay on when you disable the e All four position nosepiece stands have 2 Switch on the instrument Time Delay Shutoff the time delay shutoff enabled as a default mostly educational applications 3 Rotate the intensity regulator to the high e The LED Illumination will flash three times est level and then back to the lowest level quickly then stay on when you enable the e All five position nosepiece stands have the within five seconds Time Delay Shutoff time delay shutoff disabled as a default mostly clinical applications When you turn the power off and then turn the power on again the system will be in the last Time Delay Shutoff status Enabled or Disabled and you will not see any blinking Leica DM750 User Manual Leica DM750 User Manual Go 42 Ready Set Go Now all you need to do is change objectives set the condenser aperture and field diaphragm if you have a DM750 Koehler Stand appropriately for the objective magnification you are using and enjoy the view Leica2. e Please do not discard any of the packing materials They should be used for safely storing and transporting the instrument should the need arise Leica DM750 User Manual The Leica DM750 Ready Leica DM750 User Manual Ready 19 Substage Illumination Substage illumination Type 1 Standard illumination Type 2 Koehler illumination The Leica DM750 is available with two Adjustable condenser centering with provided Adjustable condenser centering with thumb types of Substage Illumination Identify tool screws and adjustable Koehler field diaphragm which type of Illumination you have as this will be important to know later Leica DM750 User Manual Ready 20 Attaching the Viewing Tubes Tools used e Allen key Leica DM750 User Manual There are two types of viewing tube 1 Loosen the setscrew on top of the stand Identify which viewing tube you have using the Allen key provided Type 1 Leica EZ viewing tube with integrated 2 Set the dovetail into the tripod mount and eyepieces tighten the setscrew carefully without using excessive force This precisely posi tions the viewing tube onto the optical axis of the microscope regardless of the selected rotation Type 2 Standard viewing tube with separate eyepieces Leica EZ Viewing Tube Integrated Eyepieces 1 In order to use the captive wingscrew remove the setscrew that was delivered with the stand Leica EZ viewing tub
3. Cleaning 40x and 100x objectives requires more care Note To achieve the high degree of flatness obtained with higher magnifica tion objectives the objective has a small concave front lens of fairly short radius or curvature The surface of this front lens can be readily cleaned with a toothpick cove red with a cotton tip or with a small cotton swab Moisten the cotton with commer cially available glass cleaner Wipe the front lens lightly without applying undue force or scrubbing action Make sure that the cotton tip contacts the concave lens surface Check the objective with a magni fier after cleaning Leica DM750 User Manual General Maintenance Continued If you need to remove the microscope s viewing body be careful not to accidentally touch the outer lens surface located on the underside of the body Fingerprints on this surface will reduce image clarity This lens can be cleaned in the same manner as objectives and eyepieces Illumination e The Leica DM750 uses LED illumination Therefore no lamp changing is required for the life of the microscope Care of the Microscope 46 Troubleshooting Leica DM750 User Manual Troubleshooting Troubleshooting Make sure that the socket has voltage The microscope does not respond Check the cable connections Make sure that the stand is connected to the power supply Check whether the fuse is defective and replace it if necessary see page 15
4. Care Instructions Cleaning glass surfaces Remove dust using a dry and grease free General instructions Cleaning coated parts and plastic parts e Protect the Leica DM750 microscope e Dust and dirt particles should be removed against damp vapors acids alkalis and corrosive substances Do not store chemi with a soft brush or lint free cotton cloth brush made from hair by blowing with bellows or by using a vacuum cals in the vicinity e Remove coarse debris with a moistened disposable cloth e Optical surfaces should be cleaned with a Protect the Leica DM750 microscope from lint free cloth lens tissue or cotton swab oil and grease Never grease or oil mechani e Acetone xylene or nitro containing thin moistened with a commercially available cal parts or sliding surfaces ners must NOT be used glass cleaner Follow the instructions of the disinfectant e Never use chemicals to clean colored manufacturer It is advisable to enter a service agreement with Leica Service Leica DM750 User Manual surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abraded particles Safety Regulations Accessories Maintenance and Repair Accessories Maintenance Repairs and service work Only the following accessories may be used e The Leica DM750 microscope is basically e Only original Leica Microsystems spare with the Leica DM750 microscope maintenance free T
5. The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result Leica Microsystems provide the best and most innovative imaging systems to see measure and analyze the microstructures in routine and research industrial applications materials science quality control forensic science investigation and educational applications www leica microsystems com Leica Microsystems an international company with a strong network of worldwide customer services Active worldwide Tel Fax Australia North Ryde 61 288703500 2 9878 1055 Austria Vienna 43 1 486 80 500 1 486 80 50 30 Belgium Diegem 32 27909850 279098 68 Brazil Sao Paulo 55 1127642411 11 2764 2400 Canada Concord Ontario 1 8002480123 847 405 0164 Denmark Ballerup 45 44540101 44540111 France Nanterre Cedex 33 811000664 156 05 23 23 Germany Wetzlar 49 6441294000 64 41 29 41 55 India Mumbai 91 226 1880 200 226 1880 333 Italy Milan 39 02574861 02 574 03392 Japan Tokyo 81 354212800 3 5421 2896 Korea Seoul 82 251465 43 251465 48 Netherlands Rijswijk 31 70 4132 100 704132 109 People s Rep of China Hong Kong 852 25646699 2564 4163 Shanghai 86 2160396000 21 6387 6698 Portugal Lisbon 351 213889112 21 385 4668 Singapore 65 65505999 6564 5955 Spain Barcelona 34 934949530 93 494 95 32 Sweden Kista 46 86254545 8 625 45 10 Switzerland Heerb
6. if Leica DM750 User Manual Leica MICROSYSTEMS Manufacturer Information Published in January 2015 by Leica Microsystems Schweiz AG Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland In case of questions please contact the hotline sms support leica microsystems com Leica DM750 User Manual 2 Chapter Overview afety Regulations he Leica DM750 eady are of the Microscope I Tel A J EI FA S TNG a E v FS IN Gi O CI E roubleshooting Dimensions ul Leica DM750 User Manual 3 Contents Safety Regulations Safety Concept 6 Turning on the Microscope 30 Symbols Used in This Instruction Manual 7 Complete Condenser Centering 31 Important Notes 8 Using the Condenser 32 Instructions on Use 9 Prepare to View a Specimen Slide 33 Health Risks and Dangers of Use 11 Focusing 34 Information for the Person Responsible for the Instrument 12 Viewing Tube Adjustment 35 Care Instructions 13 Koehler Configuration 37 Accessories Maintenance and Repair 14 Oil Immersion Technique 39 Electrical Data and Ambient Conditions 15 Time Delay Shutoff 41 AA Introduction 17 Ready Set Go 43 Unpacking 18 General Maintenance 45 Substage Illumination 20 Attaching the Viewing Tubes 2 Leica EZ Viewing Tube Integrated Eyepieces 22 Troubleshooting 48 Leica Standard Viewing Tube Separated Eyepieces 24 Eyecups 5 EE In
7. tions related to operating safety maintenance and accessories Your Leica DM750 microscope comes with an interactive CD ROM with all relevant user manuals Keep it in a safe place and readily accessible to the user User manu als and updates are also available for you to download and print from our website at www leica microsystems com Safety Regulations Accessories from third party suppliers You can combine individual system articles with articles from external suppliers Please read the user manual and the safety requirements of the supplier Original condition To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals Legal requirements Adhere to general and local regulations relat ing to accident prevention and environmental protection EC Declaration of Conformity Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Decla ration of Conformity Instructions on Use A The Leica DM750 microscope may be used only in closed rooms and must be placed on a solid substrate The Leica DM750 microscope can be used in clean rooms without any problems Always position the Leica DM750 micro A scope so that you can disconnect it from the power supply at any time The power cable must remain accessible at all times because
8. 31 Using the Condenser Using the condenser 1 To open and close this diaphragm simply 2 Open the iris diaphragm of the condenser The condenser is furnished with an iris turn the knurled ring on the condenser to completely at first by turning the conden diaphragm which can be adjusted to the right or left so that the line on the ring ser ring all the way to the right match the effective numerical aperture of the is aligned with the objective magnification objective used Match the line of the rotating ring with the objective magnification in use Leica DM750 User Manual Set 32 Prepare to View a Specimen Slide 1 Position a specimen slide on the specimen 2 Using the X Y stage control position the stage by sliding it under the slide grips specimen slide such that a part of the speci men is under the objective used H Slide grips hold the slide in place Slide grips Leica DM750 User Manual Set Focusing 1 Rotate the objective nosepiece using the 2 Movethe specimen stage upwards by turn knurled ring in such a way that the objec ing the coarse focusing knob as far as it will tive with the lowest magnification level is go to the maximum position rotated into the working position Coarse focus adjustment knob Leica DM750 User Manual Set 3 Look into the eyepieces and adjust the illu mination intensity to a level that is comfort able for your eyes The stand of the Leica DM750 has been H
9. 5 A USB power connector in the center of the cord wrap This can be used to power some Leica cameras or other devices requiring 5 V 1 5 A Setting the Illumination Intensity Set the illumination to the lowest A setting to start with using the control ler on the bottom left of the stand The illumi nation control knob allows you to adjust the intensity of light produced by the illumination system Set Plug in and turn on the microscope 1 Plug the power cable of the microscope into a corresponding grounded socket A grounded 3 wire cord is provided 2 Switch on the microscope using the switch at the bottom right of the microscope stand Complete Condenser Centering If you have purchased a standard Leica DM750 configuration the condenser has already been precentered by Leica Microsystems 1 Open the condenser aperture by rotating the knurled ring on the condenser to the right Make sure that the condenser is in the highest position Leica DM750 User Manual 2 Write an X on a piece of paper in the size of a business card and place it onto the light output of the microscope stand in such a way that the X is centered over the illumination 3 Look at the X through the eyepieces and center it in the field of view by tightening the setscrews with the tool included in the delivery package or for a Leica DM750 with Koehler illumination tighten the knurled screws Set Centered
10. DM750 User Manual Care of the Microscope General Maintenance General Always carry the microscope using two hands There is a handle on the back of the microscope and an undercut in the front for this purpose Leica DM750 User Manual The cord wrap allows you to wrap the cord in such a way that only the length you need is extended Keep all optical components clean Clean liness is important for maintaining good optical performance The microscope should always be covered with the plastic dust cover provided with the instrument when it is not in use Care of the Microscope If any optical surface becomes coated with dust or dirt clean the surface by blowing it off with a syringe or brushing it off with a camel hair brush before attempting to wipe the surface clean Optical surfaces should be cleaned with a lint free cloth lens tissue or cotton swab moistened with a commercially available glass cleaner It is very important to avoid the excessive use of solvents so use them sparingly The lint free cloth lens tissue or cotton swab should be moistened with solvent but not be wet enough for the solvent to seep around the lens 45 e No part of the microscope is quite so vulne rable to collecting dirt dust and oil as the front lens of the objective Whenever you encounter lack of contrast cloudiness or poor definition carefully check the condi tion of the front lens with a magnifier
11. The eyepieces will still rotate but they will be captive in the eyetubes Eyecups If you wear eyeglasses for microscope Li viewing keep the rubber eyecups folded down If you do not wear eyeglasses you may find it useful to unfold the rubber eyecups in order to help block out ambient room light Leica DM750 User Manual Ready If you have purchased a standard micro H scope configuration you will notice that the objectives are already installed on the nose piece and the substage condenser is already installed on the stand In this case go to section Operation on page 30 If you purchased your Leica DM750 in the form of individual compo nents rather than the standard configuration please continue with the Installing Objectives section on page 26 25 Installing Objectives Installing objectives When rotating the objective nosepiece A always use the knurled ring on the objective nosepiece As you rotate the nosepiece clockwise attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification Leica DM750 User Manual Ready 26 Installing the Substage Condenser Substage condenser 1 Move the specimen stage upwards as far as 3 Unscrew the two setscrews or wingscrews H The Leica DM750 has an open substage it will go by using the coarse focusing knob for Koehler stands on the condenser condenser mount therefor
12. Use the correct immersion medium Lay the specimen with the cover glass toward the top The specimen cannot be brought into focus i j Make sure that the cover slip thickness is correct and that it meets the specifica tions on the objective Dark field Make sure that a DF objective is being used The objective aperture is too high maximum 0 75 1 10 if necessary reduce No definite DF contrast is possible the objective aperture through the iris diaphragm on the objective Check the condenser centering Open the aperture diaphragm completely The image is not uniformly illuminated The objective magnification is too weak Use a higher magnification Unwanted stray light Clean the specimen and neighboring lens surfaces Leica DM750 User Manual Troubleshooting Troubleshooting Continued Polarization Bring the polarizer and analyzer into cross position until they reach maximum darkness without specimen The specimen is too thick too thin or too brightly stained Refractive indexes of the mounting medium and specimen are identical so that there is no phase jump Polarization contrast cannot be adjusted Phase contrast i The cover glass is not placed uniformly Phase contrast cannot be adjusted ae Check that the correct light ring is positioned Check the centering of the light rings Check the condenser centering Open the aperture diaphragm completely Move the mechanical stage with coa
13. calibrated at the factory so that the focus can be found from this position within 1 5 rota tions of the fine focus 4 Bring the specimen into focus using the fine focusing knob 34 Viewing Tube Adjustment Adjust the eyetubes 1 Adjust the tubes to your interpupillary distance Fold or unfold the eyetubes to decrease or increase the distance between the eyepieces until you see one illuminated circle The viewing tubes maintain a constant tube length for all interpupillary settings This means that a change of interpupillary distance does not affect parfocality magnifica tion or calibrations that depend on magnifica tion Leica DM750 User Manual Leica EZ viewing tube e If you are using a Leica EZ viewing tube which has the eyepieces integrated with the eyetubes no additional adjustments are necessary Be sure you are wearing your eyeglasses or contact lenses e If you have a Leica DM750 with stand ard illumination without Koehler field diaphragm continue with the Oil Immer sion Technique section on page 39 e Ifyou have a Leica DM750 with Koehler field diaphragm continue with the Koehler Configuration section on page 37 Standard viewing tube with two fixed eyepieces e If you are using a standard viewing tube with two fixed eyepieces no focusing eyepieces no additional adjustments necessary Be sure you are wearing your eyeglasses or contact lenses Set e If you have a Leic
14. it is intended as a power disconnect device Leica DM750 User Manual Place of use Only use the instruments in closed dust free rooms and between 10 C and 40 C Protect the devices from oil chemicals and extreme humidity If using the devices outdoors protect them from dust and moisture Never use electri cal devices outdoors Install electrical devices at least 10 cm from the wall and away from flam mable substances Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Safety Regulations Non intended use Never install any other plug or unscrew any mechanical components unless expressly instructed to do so in the instructions A The devices and accessories described in this instruction manual have been tested for safety and potential hazards The responsible Leica affiliate must be A consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Unauthorized alterations to the instru ment or noncompliant use shall void all rights to any warranty claims Instructions on Use Continued Transport If at all possible use the original packaging for shipping or transporting individual modules In order to
15. 00 240 V 50 60 Hz 5 W 3 W LED Edel a dd NR Gdf Transport shock unpacked 100g9 6 ms This instrument of safety class 1 has been built and tested in accordance Transport shock packed 800 mm free fall with the following safety requirements for electrical equipment for measu Transport vibrations unpacked 5 200 Hz 1 5g rement control and laboratory use Air pressure during use and storage 500 1 060 mbar EN 61010 1 2002 08 EN 55011 2007 A2 2010 05 AMERIGO A eI eve Snags eee EN 61010 2 101 2008 06 EN 60825 1 2008 05 Installation Category Il Overvoltage Category EN 61326 1 2006 10 LED Class 1 EN 61326 2 6 2006 10 Leica DM750 User Manual Safety Regulations 15 The Leica DM750 Introduction Thank you for purchasing the Leica DM750 Compound Microscope from Leica Micro systems This model s exclusive design features and full range of accessories make it a truly versatile high quality instrument The instrument is to be used only as described Hazards to personnel may result if used improperly Leica DM750 User Manual The Leica DM750 Unpacking e Carefully remove the microscope and any components from the packing carton e Verify that all components are intact e Check the components against the planned configuration e Optional items such as contrast accessories camera adapters cameras and carrying cases are not shipped as part of the stand ard equipment These items are delivered in separate packages
16. a DM750 with stand ard illumination without Koehler field diaphragm continue with the Oil Immer sion Technique section on page 39 e Ifyou havea Leica DM750 with Koehler field diaphragm continue with the Koehler Configuration section on page 37 Standard viewing tube with one or two focusing eyepieces If you are using a standard viewing tube with one or two focusing eyepieces you need to make some adjustments 1 Set the focusing eyepieces to 0 35 Viewing Tube Adjustment Continued If you are comfortable wearing your H corrective lenses contact lenses or eyeglasses for microscope viewing leave them on and your adjustments will be minimal 2 Using the fine focus adjusting knob focus on the specimen while looking through only one of the eyepieces when using one eyepiece that is focusable and one that is not focusable look through the one that is not focusable To help focus cover or close the other eye Leica DM750 User Manual 3 Now look with the other eye just through the other eyepiece focusing eyepiece This time focus the specimen by using the focusing capability in the focusing eyepiece When doing so do not change the height of the specimen stage 4 Grip the knurled ring on the focusing eyepiece with one hand and rotate the top of the eyepiece with the other hand until the specimen is in focus for this eye and Set this focusing eyepiece This corrects for any
17. aphragm leaves are just outside the image of the field diaphragm of the field of view Leica DM750 User Manual Set 38 Oil Immersion Technique 1 Search for the area on the specimen slide 3 Add a drop of immersion oil from Leica 4 Rotate the oil immersion objective the that you want to study to the area of the specimen slide you are objective labeled OIL to the working studying position 2 Move the specimen stage down to the lowest position using the coarse adjust ment knob Leica DM750 User Manual Oil Immersion Technique Continued Slowly move the specimen stage upwards using the coarse adjustment knob until the oil drop on the slide comes into direct contact with the lens of the oil immersion objective Hold the knurled ring on the objective turret and swing the objective back and forth to eliminate air bubbles Then bring the oil objective into the final position so that the oil drop is between the front lens of the objective and the specimen slide Leica DM750 User Manual Look through the microscope and slowly rotate the fine adjustment knob so that the Specimen stage moves upwards until the specimen is in focus If you are finished working with the oil immersion objective clean the front side of the objective the specimen slide and all other surfaces that have come into contact with the oil following the instructions in the Care of the Microscope section on page 44 Set 40
18. e the on the side of the microscope holder condenser needs to be installed 2 Move the condenser holder into the lowest position by using the condenser focusing The substage condenser mount is open knob on the left side of the stage mount Leica DM750 User Manual Ready 27 Installing the Substage Condenser Continued 4 Push the substage condenser under the 5 Move the condenser into the highest posi Specimen stage into the holder by align tion by using the condenser focusing knob ing the locating pin on the bottom of the on the left side of the stage mount condenser with the slot on the rear side of the fork 6 Tighten the two setscrews using the tool ev 0 90 1 25 OIL 81 included in the delivery package or for a Witt ty erat Koehler stand tighten the two wingscrews so that the upper lens of the condenser is centered under the objective in working position and the substage condenser is thus roughly centered H You will center the condenser more accu rately when you get to the Complete Condenser Centering section on page 31 Leica DM750 User Manual Ready 28 Set Leica DM750 User Manual Set 29 Turning on the microscope Work surface A Always use your microscope on a hard stable surface Power cord If the power cord is not already A attached attach it securely to the back of the microscope Leica DM750 User Manual USB power connector The Leica DM750 has a 5 V 1
19. e with integrated eyepieces To rotate the Leica EZ viewing tube H either loosen the set screw on the stand or replace the set screw with the optional captive thumbscrew N Make sure that the wingscrew part of the captive wingscrew has been completely separated before you use the stand Leica DM750 User Manual Ready 2 Reinstall the Leica EZ viewing tube onto the stand 3 Tighten the captive wingscrew using the wrench included in the delivery package 22 Leica EZ Viewing Tube Integrated Eyepieces Continued You can now safely rotate the Leica EZ H viewing tube by loosening the captive thumbscrew rotating the viewing tube and tightening the captive thumbscrew again The eyepieces are integrated into the Leica EZ viewing tube and preset there fore there is no need to adjust or install the eyepieces Continue with the Eyecups section on page 25 Leica DM750 User Manual Ready 23 Leica Standard Viewing Tube Separated Eyepieces 1 Insert the eyepieces into the tubes Standard viewing tubes tubes do not include eyepieces yet The standard viewing tube has a rotat H able dovetail Therefore you can now rotate the standard viewing tube freely in any orientation Leica DM750 User Manual Ready Secure the eyepieces in the tubes by tight ening the silver screws at the bottom of the tubes using a standard Phillips screwdriver not included in the delivery package
20. his Instruction Manual Warning of a danger This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to comply can cause the following e Hazards to personnel disturbances or e Functional damaged instruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following e Hazards to personnel e Functional disturbances or damaged instruments Leica DM750 User Manual Danger due to hot surface This symbol warns against touching A accessible hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Explanatory notes This symbol within the text points to addi tional information and explanations E Instructions for disposing of the instrument accessory components and consumables Safety Regulations Important Notes Description The Leica DM750 microscope meets today s state of the art of technology Nevertheless hazards may still arise during operation The potential risks are described below A Before installing operating or using the instrument it is mandatory to read this user manual In particular please observe all safety instructions Leica DM750 User Manual User manual This user manual includes important instruc
21. lain the meanings of the warning signs and messages Assign individual responsibilities for start ing operating and servicing the instru ment and monitor the observance of these responsibilities Leica DM750 User Manual Do not use the Leica DM750 microscope unless it is in perfect condition Inform your Leica representative or Leica Microsystems Schweiz AG 9435 Heerbrugg Switzerland immediately of any product defect that could potentially cause injury or harm If you use accessories made by third party manufacturers with the Leica DM750 micro scope be sure that each such manufacturer confirms the safety engineering harmless usability of the product and observe the product s user manual Modifications and maintenance of the Leica DM750 microscope may only be performed by professionals expressly authorized by Leica Safety Regulations Only original Leica replacement parts may be used in servicing the product After service work or technical modifica tions the unit must be reconfigured with observance to our technical requirements If the unit is modified or serviced by unau thorized persons is improperly maintained as long as maintenance was not carried out by us or is handled improperly Leica will not accept any liability The electric installation in the building must conform to the national standard e g current operated ground leakage protec tion fault current protection is suggested
22. mic and organiza tional aspects Leica DM750 User Manual The ergonomic design and construction of the Leica microscopy series are intended to reduce the exertion of the user to a minimum Danger of infection Direct contact with eyepieces is a A potential transmission method for bacterial and viral infections of the eye A The risk can be kept to a minimum by using personal eyepieces for each indi vidual or detachable eyecups Safety Regulations Dangers during use e The Leica DM750 microscope may only be connected to a grounded socket e The Leica DM750 microscope may not be operated unless it is in proper functioning condition The microscope illumination is in the exempt group risk group 0 according to EN 62471 2008 when used according to its intended use Never look directly into the LED beam A of the illumination equipment either with or without optical instruments as this increases the risk class Failure to observe this notice poses a risk of eye damage Information for the Person Responsible for the Instrument Information for the person responsible for the instrument Ensure that the Leica DM750 microscope is used only by qualified personnel Ensure that this user manual is always avail able at the Leica DM750 microscope Carry out regular inspections to make certain that the authorized users are adher ing to safety requirements When instructing new users do so thor oughly and exp
23. o ensure that it always parts may be used operates safely and reliably we recommend e The Leica accessories described in this user that you take the precaution of contacting e Before opening the instruments switch off manual the responsible service organization the power and unplug the power cable e Other accessories provided that these have A You can arrange for periodic inspections e Avoid contact with powered electrical been expressly approved by Leica as being or if appropriate conclude a mainte circuits which can lead to injury technically safe in this context nance contract with them e Itis advisable to enter a service agreement Service address with Leica Service In case of problems please contact us as follows e For maintenance and repair only OEM spare parts may be used Leica DM750 User Manual Safety Regulations 14 Electrical Data and Ambient Conditions Fuse replacement A In order to maintain this condition and to ensure safe operation Unplug the instrument before changing any fuses The Leica the user must follow the instructions and warnings contained in DM750 has two fuses which are located behind the power cord this instruction manual receptacle A Only use the following fuse types 5x20 mm 1 A 250 V fast Environment acting fuse 13RFAG30003 Temperature for use 10 C 40 C Storage temperature 20 C 52 C 50 F to 104 F Electrical data in T T ui J Input 1
24. prevent damage from vibrations disassemble all moving parts that according to the user manual can be assembled and disas sembled by the customer and pack them sepa rately Leica DM750 User Manual Disposal Once the product has reached the end of its service life please contact Leica Service or Sales about disposal Please observe and ensure compliance with the national laws and regulations that implement for example the EC Directive WEEE E Like all electronic devices this instru ment its accessory components and consumables must never be disposed of with general household waste Disposal must comply with locally applicable laws and regula tions Safety Regulations Integration in third party products When installing Leica products into third party products the manufacturer of the complete system or its dealer is responsible for follow ing all applicable safety instructions laws and guidelines Health Risks and Dangers of Use Health risks Workplaces with microscopes facilitate Li and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken e Optimum workplace layout e Frequent changes of activity e Thorough training of the personnel giving consideration to ergono
25. rugg 41 71 726 34 34 71 726 34 44 United Kingdom Milton Keynes 44 800 298 2344 1908 577640 USA Buffalo Grove Illinois 1 8002480123 847 405 0164 131DE13060EN 1 Copyright by Leica Microsystems Schweiz AG CH 9435 Heerbrugg 2015 Subject to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH eua MICROSYSTEMS
26. stalling Objectives 26 Dimensions 51 Installing the Substage Condenser 27 Leica DM750 User Manual A Safety Regulations Safety Concept The individual modules of the Leica DM micros copy series include an interactive CD ROM with all relevant user manuals in several languages Keep it in a safe place and readily accessible to the user User manuals and updates are also available for you to download and print from our website at www leica microsystems com This user manual describes the special func tions of the individual modules of the Leica DM microscopy series and contains important instructions for their operational safety main tenance and accessories The Safety concept booklet contains addi tional safety information regarding the service work requirements and the handling of micro scope accessories and electrical accessories as well as general safety instructions You can combine individual system articles with articles from external suppliers Please read the user manual and the safety requirements of the supplier Leica DM750 User Manual Before installing operating or using the instru ments read the user manuals listed above In particular please observe all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals Safety Regulations Symbols Used in T
27. vision differences between your right eye and left eye 5 Now switch to an objective with a high magnification level no oil objective and bring the microscope into focus while look ing through the eyepieces with both eyes The higher magnifications have a shal lower depth of field Therefore after focusing with a high magnification you will find that when you change to lower magnifi cations you only have to adjust the fine focus slightly if at all e f you use a DM750 with standard illumi nation continue with the Oil Immersion Technique section on page 39 e If your DM750 has a Koehler configura tion please continue with the next section Koehler Configuration on page 37 Koehler Configuration If your Leica DM750 is fitted with a field diaphragm for Koehler Illu 2 Focustheleavesofthe field diaphragm using the condenser focusing mination use the following procedure to assure condenser cente knob on the left side of the stage mount ring and focus 1 Connect the Koehler field diaphragm to the base of the microscope so that the diaphragm leaves are in the field of view when you look through the eyepieces Condenser focusing knob Leaves are in sharp focus Close the Koehler field diaphragm Close the Koehler field diaphragm Leica DM750 User Manual Koehler Configuration Continued 3 Turn the condenser centering wingscrews simultaneously to center 4 Open the field diaphragm until the di
28. xial drive all the way to the left Specimen stage Manually press the screw that holds the mechanical stage even farther to the Positioning range of the stage in the x direc left as far as it will go Then move the mechanical stage with coaxial drive all the tion decreases after working a long time way to the right Manually press the screw that holds the mechanical stage even farther to the right as far as it will go Leica DM750 User Manual Troubleshooting Dimensions Leica DM750 User Manual Dimensions 50 397 976 15 67 15 33 E 281 613 11 09 Dimensions Leica DM750 User Manual 3250 def i SL UL Es DEEP TT 11 08 219 598 8 65 Dimensions Leica DM750 User Manual 304 078 389 506 15 33 to SE EE JET qt pa LI a a SF eni I j Ni i Thee iI oe Ti T 7 ji ti Leica DM750 User Manual Dimensions The statement by Ernst Leitz in 1907 With the User For the User describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems We have developed five brand values to live up to this tradition Pioneering High end Quality Team Spirit Dedication to Science and Continuous Improvement For us living up to these values means Living up to Life INDUSTRY DIVISION
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