Operating Instructions Biological Safety Cabinet MSC
Contents
1. calibration routine status of the calibration function device controlled UV disinfection optional remaining disinfection time Values are output as integers B Keys for switching operational functions on or off C Status indicators show the operational status of front window work position airflow reduced blower speed SmartFlow Indicator 1 113 2145 od E Co Gat Fig 5 Operating and display elements 50128856 04 2011 Description of the device Operating Instructions Biological Safety Cabinet MSC Advantage 4 5 Sample chamber access Fig 6 The manually movable front window 1 made of multi layer security glass seals the safety sample chamber front side up The sample chamber is accessible through various positions of the front window For operation Work position with opening height A for access to the sample chamber during the work process maximal opening height B for loading the sample chamber position C closed state closed front window and reduced blower speed Co NOTE Status indicator The two positions A and C are determined by switches and output as status indicators on the display see Section 6 1 1 For cleaning maintenance SmartClean window cleaning position with opening height D for cleaning and disinfecting the upper section of the front win dow For this purpose the front window can be lowered beyond closed position C so2. Exterior housing Steel painted Device backpanel Stainless steel steel painted Operating panel and indicator a om Polyethylene Workplates Stainless steel Stainless steel 50128856_04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage Disposal WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling disposal com panies in each EU Member State and this product should be discarded or re cycled through them For further information on Thermo Scientific s compliance with these Directives contact the recyclers in your country and information on Thermo Scientific products wich may assist the detection of substances sub ject to the RoHS Directive at www thermo com WEEEROoHS 50128856 04 2011 47 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 12 Technical data 48 oo mis MSC 12 MSC 1 Exteriordimensions S SCSCSSSCCY CSCS mm 1000 7900 mm 502 mm 1522 nterior dimensions Width Depth Height 1200 1500 1800 630 d Oo O 313 3 S13 3 ront window Working position 313 313 N an a on Opening max Working area height fixed height stand 3 d on oO Seat position Stand height fixed height stand
3. SCIENTIFIC A Operating Instructions i Biological Safety Cabinet MSC Advantage Valid 04 2011 50128856 SCIENTIFIC Copyright 2011 These operating instructions are protected by copyright Rights resulting thereof particularly reprint pbhotomechanical or digital postprocessing or reproduction even in part are only allowed with the written consent of Thermo Electron LED GmbH This regulation does not apply to reproductions for in plant use Trademarks MSC Advantage and SmartFlow are registered trademarks of Thermo Scientific Thermo Scientific is a brand of Thermo Fisher Scientific Inc All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers Thermo Electron LED GmbH Robert Bosch StraBe 1 D 68505 Langenselbold Germany The Thermo Electron LED GmbH is a subsidiary from Thermo Fisher Scientific Inc 81 Wyman Street Waltham MA 02454 USA 90128856_04 2011 90128856_04 2011 1 General 9 0 eee mcr er ene a a 6 1 1 Instruction of the operating personnel cccccseeeeeeeeeeeeeeeeeeees 6 1 2 Applicability of the instructions cc ceecceceeeeeecaeeeeeeseeeeeeeaeeeeeens 7 Tied Way e E O 7 1 4 Explanation of symbols ccccecccccceseeeeeeeesaeeeeeeseaeeeeeeeeseeeeeeeeeeas 8 1 4 1 Symbols used in the operating inStruCtiONnS cccseeeeeeeeeees 8 1 4 2 Symbols ON the device ccceececcecceeeeee
4. 4 2 Safety system The safety system comprises a combination of protective and alarm systems that ensure maximum personal and material protection Safety systems Vacuum sealed air system A vacuum sealed air system in combination with HEPA filters for downflow and exhaust air forms the basis of the safety system for personal and ma terial protection Personal protection Air aspired from the exterior along the entire working opening at a constant high velocity prevents e agents leaking through the working opening of the chamber As the exterior air pressure around the device exceeds the pressure of the internal air system vacuum sealing it ensures e agents cannot be released to the exterior in the case of a leak in the cabinet housing Material protection A steady airflow within the air system ensures e a constant downflow allowing the HEPA filters to remove contaminants so that the samples are always surrounded by ultrapure air e harmful particles are not carried over through the sample chamber protection from cross contamination HEPA filters The downflow i e the air circulating within the device and the exhaust air air that is released to the exterior are cleaned by HEPA filters HEPA High Efficiency Particulate Air Filter Safety lockout To protect from UV radiation the optional UV disinfection routine can be run only if the front opening is closed During UV disinfection the front opening safety
5. down until the grid segment can be removed from below the counterholder 3 of the wall To install the grid segment 2 first place it behind the fixed points 4 then push the retaining tab 1 down and against the counterholder 3 of the wall so that the counterholder engages in the tab Co NOTE Inlet air protection Do not operate the device without inlet air protection Prior to any start up of the device make sure that the inlet air protection is in stalled Fig 16 Protective grid segment 90128856_04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage 10 1 Inspection The SmartFlow Insicator the test routine for the biological safety cabinet deter mines the cabinet s system status by way of adjusting various device parameters e An inspection of the device should be performed if the SmartFlow Indicator displays steady 2 yellow LED s e Regardless of the SmartFlow Indicator the biological safety cabinet should be inspected annually The annual inspection comprises the following checks e Electrical safety in accordance with national regulations e Functional test of the device e Checking all components for possible damage e Checking the filter state Co NOTE Perforated plate The perforated plate at the sample chamber ceiling serves as the protection of the down flow filter and prevents refluxing While scanning the filter surface for a leak test the
6. stand 2 Q D A o M Q O c a gt 3 O Oo O Diameter Distance lower edge Ww 3 o ot U fe ot h M D ad mt gt ea O 3 3 p 3 iS 3 3 N ND J y N Ke Diameter N tO Q Distance lower edge J A rt 3 O O 3 o D O A Ee D 2 Bushing 1 Bushing 2 SmartPort 3 3 Sisis yN A N O O 50128856_04 2011 50128856_04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage Description mMmscog msc12 Msc15 MSC18 NXolume o o S S Floorpan Ji 5 55 3 o Weights 30 Loads Max load per working area kg 25 module Max load on overall working m area Ambient conditions Description MSC 0 9 MSC 1 2 MSC 1 5 MSC 1 8 Temperature Max ambient temperature during operation Min ambient temperature C during operation Humidity Max humidity operation 90 non condensing Max gumidity storage Heat dissipation to environment Room temperature 20 C 0 15 0 2 0 28 0 34 Room temperature rise Above room temperature with K 25 lt 2 lt lt 2 window closed The noise level was determined in accordance with EN Noise level ISO3744 The sound pressure level was measured at a distance of 1 m in front of the working opening The measurement uncertainty is within a range of 2 dB 50 Operating Instructions Biological S
7. 200 mm 8 in The location must be equipped with an appropriate ventilation system see Section 3 2 For the valves may be installed a lateral distance of at least 300 mm is required to ensure accessibility for the installation see Chapter 4 6 The temperature within the room must be between 15 C and 40 C 49 F and 104 F The relative humidity in the vicinity of the device must not exceed 90 Co NOTE Ambient conditions If ambient conditions vary from those descri bed above please contact Thermo Fisher Scientific for assistance in installing the device Co NOTE Temporary storage If the device is stored only temporarily up to four weeks the ambient temperature may be between 20 C and 60 C 4 F and 140 F at a relative air humidity of up to 90 For lon ger storage periods the location requirements apply 50128856_04 2011 50128856_04 2011 3 2 Room ventilation The room ventilation should preferably be a ventilation system that complies with the national requirements for the application e The inlet air and exhaust air openings of the room ventilation must be located so that drafts are prevented from impairing the function of the safety cabinet air system Coupling to laboratory exhaust systems Coupling biological safety cabinets to a laboratory exhaust system air that spills of biological agents must be discharged to prevent inflow to the installation space The ventilation system of
8. 36 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 7 4 Work rules The observance of work rules ensures a minimum of operational safety when handling the biological safety cabinet Before starting an operation e Take off jewelry e Puton required personal protective gear e g hand face or body protection e Clean and disinfect sample chamber surfaces at regular intervals During operation e Place samples only within the defined work area of the workplate e Do not place unnecessary items into the sample chamber e Use only disinfected and cleaned accessories for the work process e Do not cause air turbulence by quick hand arm or body movement in the sample chamber or in front of the work opening e Do not place accessories into the sample chamber that cause air turbulence or emit excessive heat e Do not block air circulation at the ventilation slots of the workplate Sitting posture during work Fig 15 To prevent risks to health a height adjustable working chair with an adjustable seat back should be used during extended work periods at the biological safety cabinet A When the forearm rests on the armrest is should be in a nearly horizontal position B When the upper leg is in a horizontal position the angle between upper and lower leg should exceed 90 To ensure a compensation between floor and sitting height a footrest DIN 4556 should be used The minimal effective surface o
9. IS IN WORK POSITION is illuminated The markings on the side guide rails and the lower edge of the front window are at the same height No audible alarm signal e The air system blowers are switched on to ensure steady airflow The green status indicator AIRFLOW STEADY is illuminated e The sample chamber illumination is enabled e The power supply for the sample chamber outlets is enabled lf the internal power supply is ON the yellow status indicator INTERNAL POWER SUPPLY ACTIVATED is illuminated e The switching state of the potential free contact monitor contact is pass if e g the gas supply solenoid is driven via this contact gas is supplied in this mode only e The display can show values for operating hours downflow velocity and ex haust air velocity 33 34 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 6 Handling and control Standby mode The front window can be lowered to seal the sample chamber The air system output is reduced to match the lower air requirement The front window is closed The air system operates at reduced output The red status indicator FRONT WINDOW NOT IN WORK POSITON iS illuminated The yellow status indicator AIRFLOW REDUCED is illuminated The red status indicator AirFLow NOT sTEADY is illuminated The sample chamber illumination is enabled The internal power supply in the sample chamber is enabled Ifthe internal p
10. i Customer Center Germany Telefax 08001 112 114 Declaration Robert Bosch Str 1 www thermofisher com D 63505 Langenselbald Invoice recipient Customer no Location address Forwarding address ee ee Safety Declaration Dear customer When using biological and chemical agents within and ouside of devices hazards to the health of the operating personnel may be present and contamination of the surroundings of the device may occur when service or repair works are carried out Within the scope of national and international legal regulations such as responsibility of a company for the protection of its employees responsibility of the operator for the operational safety of devices all possible hazards must absolutely be prevented Prior to any calibration service and repair works prior to any relocation of a device and prior to the shut down of a device the device must be decontaminated disinfected and cleaned as required by the work to be carried out Therefore we ask you to fill in this certificate of decontamination before you start with the required work Yours sincerely Thermo Electron LED GmbH Works to be carried out please mark where applicable Prevented maintenance Filter replacement Repair work Transfer to new location Calibration Transport Declaration of possible contamination please mark where applicable The device is clear of biological material The device is clear of dangerous chemi
11. lockout is activated and prevents harmful UV radiation from being emitted from the sample chamber Circuits can be driven via potential free contacts monitor contacts e g a solenoid that switches automatically upon cancellation of the work mode Warning system Airflow monitoring Airflow monitoring determines the velocity of the airflow in the sample chamber as well as the inflow velocity of the air aspired from the exterior through the working opening As soon as airflow velocities move above or below a spe cified safety value a signal is transmitted to the alarm system Visual and audible alarm system The warning system constantly monitors the safety relevant device functions e Inflow velocity of the air aspired from the exterior e downflow velocity e work position of the front window If the warning system detects changes to one of these device func tions it issues e an audible and a visual alarm signal 50128856_04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage Description of the device e Position monitoring The position sensors detect the opening state of the front window and indicate whether the window is open or closed in the work position e SmartFlow Indicatordisplay The SmartFlow Indicator displays the compensation capa billity of the exhaust control if the window is in working po sition and blower on 4 3 Filter system Fig 4 The filter system consists of two HEPA
12. the biological safety cabinet itself are not to be influenced If a coupling to a laboratory exhaust system is installed it must be ensured that no harmful air flow is created in the installation space The volume of air discharged by the exhaust system must be tracked accordingly into the installation space It should be noted that the equipment air flow itself is not affected The on site exhaust system should be dimensioned so the air velocity scale in the draft interruptor and exhaust air plenum is sufficient that the equipment exhaust air has no air resistance In states where the coupling to a laboratory exhaust system is regulated by further provisions the relevant national regulations must be observed AN CAUTION Installation test During the initial start up of the biological safety cabinet an initial installation test must be performed The installation location of the biological safe ty cabinet with connection to a technical ven tilation or with a coupling to laboratory exhaut system and additional installedexhaust accessories are not to be changed Every modification of the installation location requires a new installation test see chapter 5 6 Operating Instructions Biological Safety Cabinet MSC Advantage 13 14 Operating Instructions Biological Safety Cabinet MSC Advantage hermo Installation 3 3 Correct location 1 3 Choose a draft free location where the biological safety cabinet does no
13. 40 9 3 2 UV disinfection using a mobile UV device optional 40 9 3 3 Changing UV disinfection time cccccececeeeeeeeeeeeeeeeaeeeeeeeeens 40 9 4 Disinfection with formaldehyde ccccceecseeeeeeeeeeeeeeeeeeeeeeeeees 41 9 5 Cleaning the exterior surfaces ccccceeeeeeeeeeeeeeeeeeesaeeeeeeeeaaaeeeess 41 9 6 Cleaning the front WINdOW cccccccseseseeeeeeseeeseeeessaeeeeeeeesaaeeeess 41 9 7 Cleaning the floorpan cccccccsseeeececeeeeseeeeseeeeeeeeeseaaeeeeeseeaaeeeess 42 9 8 Cleaning the protective grid ccccccccssssseeeecceeseceeeseeseeeeeessaeeeess 42 WO Mam enane E c 43 Oa oe ea a 43 VO a E A AE P E EE E ET E E 43 10 2 1 Sample chamber illumination cc eee ceceeeeeeeeeeeeeeseeeeeeeeaees 44 10 2 2 Optional UV lamps csiisiiserunnianscrrriniuncnerennnninnanininndiaiininia 44 10 3 Retrofitting and repairs n nnnnonnnnnennnnnnnnenrnnnnsrnrennnnrnsrnnrrsnnrennne 44 10 4 Exchanging SmartPorts cccccccccsseeeceeeeeeseeeeeessaeeeeeeeseeneeeeeeeas 45 WY ISRO SAN n R 46 11 1 Disposal procedure ccccccccceccceeseeeeeeeeeeeeseeeeeesaeeeeeeeesaaeeeeeeenas 46 12 Technical dalana ai E E Eii 48 WS DEVICE IOU reren E E A meneeeae 52 14 Certificate of decontamination cccceeeeseeeeeeeeeeeeeeeeneeseeeeeeeeeeeeeees 53 Fig 1 Device arrangement in the operating room 08 14 Fig 2 Lift POINTS 2 00 ccc ccceeeececeeeeeeeee
14. UV key The display shows the operating hours again 50128856_04 2011 50128856_04 2011 9 4 Disinfection with formaldehyde Procedure For gas disinfection formaldehyde is evaporated in the tightly sealed sample chamber The quantity of the formaldehyde used depends on the sample chamber volume of the cabinet version to be disinfected see Technical Data Per cu bic meter of sample chamber volume at least 5 g formaldehyde must be evapo rated with 20 ml water corresponds with 25 ml of a 20 formaldehyde solution The formaldehyde evaporates immediately when its boiling point is reached The required reaction time is at least 6 hours After the required reaction time the formaldehyde should be neutralized by evaporating a 25 ammonium soluti on 10 ml per cubic meter of sample chamber volume Ambient conditions and accessories The temperature at the cabinet location should be approx 21 C the relative humidity should be between 60 and 85 To evaporate the solution a heating device with a container is required Co NOTE Disinfection procedure A disinfection with formaldehyde must be performed in accordance with the specificati ons of EN 12469 As this procedure has considerable risks it must only be performed by specially trained and authorized service personnel 9 5 Cleaning the exterior surfaces Wipe the exterior surfaces of the device clean using a solution of TAP water and commercially available dishwa
15. UV lamp 1 is installed to the sample chamber ceiling immediately behind the front win dow The UV lamp should be replaced after 1500 operating hours 1 Switch the device off disconnect it from the power supp ly system and protect it against accidental reconnection 2 Move the front window to the maximum opening position 3 Wear protective gloves to prevent skin fat residues from burning into the lamp tube The UV lamp is installed in rotating sockets To remove ro tate the lamp counter clockwise to disengage the latch and remove it from the sockets 4 To install slide the lamp contact pins into the rotating so cket grooves and rotate the lamp clockwise to latch the so ckets 10 3 Retrofitting and repairs External communication systems e g failure report systems or components for supplying media such as gas solenoid val ves can be retrofitted and integrated into the device control Co NOTE Retrofitting and repairs All retrofitting and repair works are interfe rences with the safety system of the device Particularly modifications to the filter system Fig 17 and resulting changes of the airflow may im Amp Tepiecentenl pair personal and material protection Such work must be carried out only by authorized service personnel hermo Operating Instructions Biological Safety Cabinet MSC Advantage 10 4 Exchanging SmartPorts Co NOTE Contamination In order to prevent con
16. afety Cabinet MSC Advantage hermo Electrical data Voltage Current Leakage current IEC1010 mA EN 61010 lt 3 9 Motherboard fusing O A 2xT 16A gt Outlet fusing 2xT5A On site fusing Circuit breaker B16 Fuse T 16 A Power 1700 0 2000 0 Protection class SS Protectiontype o IEC 1010 EN 61010 el IEC 1010 EN 61010 Connecting lines Mains connection Cable 3 7 m IP 20 50128856_04 2011 Operating Instructions Biological Safety Cabinet MSC Advantage Airflow system Description MSC 0 9 MSC 1 2 MSC 1 5 MSC 1 8 Inflow Downflow 0 45 0 32 Air volume Overall volume flow 1218 1523 1827 Downflow volume flow 1037 1244 Exhaust air volume flow 583 HEPA H 14 according to DIN EN 1822 Glas fiber fleece Type Material Separability at 0 3 um 99 999 particle size a a Separability in MPPS 99 995 Width 1220 1525 Depth 457 Height 4 width 510 510 JE Depth Fight 50128856_04 2011 51 FIC Thermo SCI Operating Instructions Biological Safety Cabinet MSC Advantage LLOZ YO 9988Z40S Work carried out Device type ooo o Pannumeer SSS Serial number Service number O Location Operator snote A 52 90128856 _04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage SCIENTIFIC Certificate of decontamination Hairi SCIENTIFIC 1 1 Thermo Electron LED GmbH Telephone 0 8001 112 110
17. al address Germany Thermo Electron LED GmbH Robert Bosch Stra e 1 D 63505 Langenselbold Inquiries from Germany Phone Phone Sales 0800 1 536376 Phone Service 0800 1 112110 Fax Sales Service 0800 1 112114 EMail info labequipment de thermofisher com Enquiries from Europe Middle East and Africa Phone 49 0 6184 90 6940 Fax 49 0 6184 90 6772 EMail info labequipment de thermofisher com Postal address USA Thermo Fisher Scientific 275 Aiken Road Asheville NC 28804 USA Enquiries from North America Phone 1 800 879 7767 Fax 1 828 658 0363 EMail info labequipment de thermofisher com Enquiries from Latin America Phone 1 828 658 2711 Fax 1 828 645 9466 EMail info labequipment de thermofisher com Enquiries from Asia Pacific Phone 852 2711 3910 Fax 852 2711 3858 EMail info labequipment de thermofisher com Thermo Operating Instructions Biological Safety Cabinet MSC Advantage 1 1 Data of the MSC Advantage and the documentation Device identification Device Name Biological Safety Cabinet Model MSC Advantage Model lines 0 9 1 2 1 5 1 8 Model 1 2 from serial devices 41070608 Model 1 8 from serial devices 41070639 Assignment of the product documentation User manual 501268856 Availability 04 2011 Certification and Quality Audit Conformity CE conformity marking 1 2 Instruction of the operating personnel These operating instructions describe the biological safety cabinet
18. and the ple num including the filters must be sterilized using formaldehyde 1 After the device has been decontaminated close the front window completely 2 Disconnect the device from the power supply system 90128856_04 2011 37 38 Operating Instructions Biological Safety Cabinet MSC Advantage 9 1 Decontamination procedure Several procedures can be applied for decontaminating the biological safety cabinet Which procedure is selected depends on e the potential risk imminent in the agents e the degree of purity required by an experiment or by a work process Possible decontamination procedures Wipe spray disinfection is the standard disinfection procedure for cabinets used for microbiological experiments UV disinfection is particularly suited as an intensifying additional disinfection after a wipe spray disinfection Sterilization with steam can be used for treating the removable stainless steel components Examples of autoclavable components are the bezels of the UV lamps the workplate or workplate segments the armrests and the protective grid see Section 9 6 9 7 Disinfection with formaldehyde can be performed if a sterile sample chamber is required for the work process This sterilization procedure is mandatory e when filters are replaced e when the device is shut down e when the device is discarded 9 2 Wipe spray disinfection The wipe spray disinfection is performed in three stages
19. as recommended by the manufacturer then operate the biological safety cabinet for at least 15 to 20 minutes in the work mode so that released aerosols can be absorbed by the filters Cleaning 1 2 3 Remove dirt residues and deposits thoroughly using a solution of tepid water and commercially available dishwashing agent Wipe the surfaces clean using a clean cloth and plenty of clear water Remove the cleaning liquid from the floorpan and wipe all sample chamber surfaces dry Final disinfection 1 Again spray disinfectant on all sample chamber surfaces or wipe the sur faces clean using disinfectant 2 Allow disinfectant to react as recommended by the manufacturer Operating Instructions Biological Safety Cabinet MSC Advantage 39 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 9 3 UV disinfection after a wipe spray disinfection A UV disinfection can be performed either by using the optional integral UV lamps or by using a mobile UV device 9 3 1 UV disinfection using the integral UV lamp To start the UV disinfection routine 1 Close the front window completely the air system operates in the reduced mode yellow LED illuminated 2 Start UV disinfection using the UV key at the operating panel Keep the UV depressed until the display alternatingly shows dIS and the re maining disinfection time To interrupt cancel the UV disinfection routine 1 Press the UV key the disp
20. ating panel Co NOTE Protection from UV radiation To protect from UV radiation the UV lamp can be activated only when the front window is completely closed When the front window is opened the UV di sinfection routine is cancelled immediately 4 9 Working area The standard equipment comprises the segmented work plate The workplate segments are placed onto the frame above the sample chamber floorpan Co NOTE Workplate condition Use properly plane lying workplates only Replace deformed workplates Pay attention to the maximum work load see sction 12 Fig 9 The working area A for optimal product protection extends over the entire width B and depth C of the workplate 1 The two armrests 3 are positioned centrically to the working area at distance D 20 cm from each other For attachment each arm rest is inserted into the first perforation track 2 of the workplate Co NOTE Using the armrests Safe working in the work mode of the device see section 6 3 is ensured only if the armrests are used correctly Fig 8 UV lamp unit Fig 9 Working area on the workplate armrests 50128856_04 2011 5 1 Initial operation Prior to the initial operation the biological safety cabinet must be subjected to an installation test Correct assembly and instal lation performed by the operator are essential for good start up Upon the initial connection to the power supply syste
21. cal substances The device is clear of radioactivity The device is clear of other dangerous substances The device is clear of cytostatic agents Certification Prior to carrying out the required work we have decontaminated disinfected and cleaned the device as described in the operating instructions of the device and in accordance with nationally applicable regulations The device does not present any hazards Revision 1 TRermofisher certified 18 08 08 MW Status Approved Ident Nr 50077599E File name P50077599E pro Date legally binding signature stamp Internet http www thermofisher com 356_04 2011
22. e MSC Advantage and apply to the models MSC 0 9 1 2 1 5 1 8 The biological safety cabinet has been manufactured in keeping with the latest tech nological developments and has been tested before delivery for its correct function It may however present potential hazards if it is not used according to the intended purpose or outside of operating parameters Therefore the following procedures must always be observed to prevent accidents e The biological safety cabinet must be operated only by trained and authorized personnel e For any operation of this device the operator must prepare clear and concise written instructions in the language of the operating and cleaning personnel ba sed on these operating instructions applicable safety data sheets plant hygiene guidelines and technical regulations in particular e which decontamination measures are to be applied for the cabinet and accessories e which protective measures apply while specific agents are used e which measures are to be taken in the case of an accident e Repairs to the device must be carried out only by trained and authorized expert personnel 1 3 Applicability of the instructions e The contents of the operating instructions are subject to change without further notice e Concerning translations into foreign languages the German version of these operating instructions is binding e Keep these operating instructions close to the device so that safety instructi
23. e device contains an RS 232 connection 23 for a PC and two fuse hol ders 22 Light dome 4 for the sample chamber illumination unit equipped with one fluorescent tube model MSC 1 2 or two fluorescent tubes model MSC 0 9 1 5 1 8 The optional device integral UV lamp is installed to the ceiling at the front section of the sample chamber Operating panel 5 with function keys and indicators Front window 7 with two handles 6 Bushings 8 and 9 in the side panels 3 on each side The bushings 9 can be used to install media valves 11 the bushings 8 of type SmartPort are provided for laying cables or hoses into the utility chamber Stand 10 optional Workplate segments 13 with 2 armrests 14 A one piece workplate and spe cial workplates are available as optional accessories Internal outlets 15 for the power supply of accessories optionally one of the outlets can be equipped with an adapter 17 for mobile UV devices Test hoses for the downflow unit 16 at the left side of the sample chamber and for the exhaust air unit 12 at the right side of the sample chamber C NOTE Test hoses Do not remove the caps of the two test hoses for checking downflow and exhaust air 50128856_04 2011 90128856_04 2011 Operating Instructions Biological Safety Cabinet MSC Advantage Fig 3 Overall view 18 Operating Instructions Biological Safety Cabinet MSC Advantage
24. e predisinfection e cleaning e final disinfection Recommended disinfectants Cc NOTE Compatibility Chloride containing disinfectants may damage some surfaces Therefore use only chloride free disinfectants or a disinfectant with a low enough chloride content to have been proved harmless for stainless steel finishes Disinfectants with an alcohol content of more than 70 may cause embrittlement of plastic components after extended exposure Use only disinfectants with a low alcohol content When using a disinfectant with an alcohol content of more than 70 the release limit of 200 g within 2 hours must not be exceeded Also suited are disinfectants based on quater nary ammonium compounds 50128856_04 2011 90128856_04 2011 Cleaning and decontamination Predisinfection 1 2 3 Remove all samples from the sample chamber and store them properly Remove accessories from the biological safety cabinet and disinfect them using the disinfection procedure recommended by the manufacturer The workplate and stainless steel components can be removed from the sam ple chamber and disinfected separately For predisinfection spray disinfectant on all sample chamber surfaces or wipe the surfaces using disinfectant Do not remove the optional UV lamps from the sockets wipe them thorou ghly using a damp cloth Switch the device to work mode move the front window to the work position Allow disinfectant to react
25. eeeesaeeeeesaeeeeeeneaseeensees 15 Fig 3 Overall ViQW cc ceecccccccssssececceeeeeeeeeseeeeeseeesseaseeeesssaaeeeess 17 Fig 4 Filter system with downflow and exhaust air filter 19 Fig 5 Operating and display elements ccccceeeeeeeeeeeees 20 Fig 6 Sample chamber Opening ccccccccceesssssseeeeeeeeeeeaes 21 Fig 7 Supply interfaces cecccccccccsssesssseeeeeeeessseesseeeeeeeeesaas 22 Fig 8 UV aM unit ou ec ccccceeeeeeeeeeeeeesaeeeeesaeeeeeesaeeeeeneees 24 Fig 9 Working area on the workplate armrests cccce 24 Fig 10 Stand installation 20 0 ccccccccccsssssseeeeeeeeesseeseeeeeeeeeeeaas 25 Fig 11 Unlocking the transport protection ccceceeseeeeeeeeeees 26 Fig 12 Operating panel ccccccccsssseeeeeeeesesseeeeeeeeeeeeaas 30 Fig 13 Operating panel Il ceeeeeeeeeeeeeesseeeeeeeeeeeeeaas 31 Fig 14 Display upon Start Up cc cceceeceeeeseeeeeeeeseeeseeeeeeneees 32 Fig 15 Sitting posture 2 0 eee ecccccceeseeeeeeeeeeseeeeeeeeeeeeessaaeeeens 36 Fig 16 Protective grid segment ccccceecceecseeeeeeeeeeeeeneeeeeeneees 42 Fig 17 UVlamp replacement ccccccccseseeeeeeseeeeeeeeeseeeeees 44 90128856 04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage 1 General notes 50128856_04 2011 The following are the addresses of the international FisherScientific Sales Organisations Post
26. ees 31 6 1 4 Failure messages ccccceeecccceescecceeeeeeceeeeeceeeeeeseeeessseseeenes 32 62 WC VIGS starl UD citar hertaeca sncasocn biennial eaaentunvtintinginamnacavincsau eeutemctiadanns 32 6 3 Description of the operating modes cccceccccceeeeeeeeeeeeeseeeeees 33 Z Operaatio ssns sane edness ew erento ete eee 35 7 1 Hygiene preparations for the sample chamber e 35 7 2 Loading the sample Chamber ccccsseeecceeeeeeeeeeeeeeeeeeeeeeeaaeees 35 7 3 Response to failure MESSAGES ccccccseeeeeeeeeeeeeeseeeeeeneeeeeenaees 35 7 4 WorkrulesS aoa ccc secr asc atie ciccintecetieciesssuaienbebadinettadaueiseuenudsnaeadseecasesteteunedie 36 Operating Instructions Biological Safety Cabinet MSC Advantage Operating Instructions Biological Safety Cabinet MSC Advantage hermo 0 SNU LOOWN ccn E enere eet er et 37 6 1 Interr pt ng AN operatiOM sssrinin ik iarain iia 37 8 2 Shutting the device down cccccccecccseseeeeeeeeeseeeeeesaeeeeeeeesaaeeees 37 9 Cleaning and decontamination cccccccceessseeeseseesseeeseeesseeesseeenseees 38 9 1 Decontamination procedure cccccceeseeceeeceesseeeeeseeeeseeeeseaneeeeees 38 9 2 Wipe spray disinfection cccccccecccccccseeeeeeeeseeeeeeeeeeeeeeceeeesaaeeees 38 9 3 UV disinfection after a wipe spray disinfection ccceeeeeeeeeeees 40 9 3 1 UV disinfection using the integral UV lamp eeceeeeees
27. eesaeeeeeeessaeeeeeeesaaeeeees 9 1 5 Use ofthe device rice c casnce sed oesnedadcesustidvesandctentsseadeneeianeessttieecedel 10 ko CONC USE a a eee eee ee 10 1 5 2 Incorrect USE seadrudecteanaehaincdaiacteanetinepiuteccuel EELNE eant Na a E 10 1 6 Standards and safety regulations cccceececeeeeseeeeeeeeeeeeeeeeeeeas 11 PCI OY ooon Aea veweesetvieesssensencessumewanecesensneccssnerss 12 2 1 Standard components cccccccccssesesecceeeeeeeueeeeeeeeeeeeeeaaeseeeeeeees 12 2 2 Acceptance INSPECTION ccccsesccccseseeeccseeecceeseecceseeessaseeessaeees 12 2 3 Transport security lock and device packaging cccccseeeeeees 12 3 VSTAMAUION sires E RE 13 3 1 Ambient conditions cde csec2senccds aeccotmndeiercte2eddecendmnasctedepancesoeeneadioned exes 13 92 ROOM VEMUNATOMN soi cose cee xcshcaseessenasedenwehencsedetesectsactanc bcdeceaceosestzcacch 14 39 COMECIIOCAUON siscerassexinetcndsecnvadaiaahtendesesteaaradtfieddeqntsdazectbtedenartnacics 14 3 4 Installation IN series cecccccssececceeseeeceeseeeceeseeeseuseeessageeessaaeees 14 GeO WATS OOM e E 15 4 Description of the device ssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn mnnn 16 AN Overall VIEW senccescsicseescdosdcndetanwencedsbaesastarsenadluniecdahendeoaahewecoantenteedud 16 42 SOlely Syste ceced acteedevetiecsandaped onesie E 18 4 3 Filter system rsadicnsaranbanddieavavavns usec nidsiudanshXackendseancincussncnentisandamdut
28. evice counterweight during transport Protective packagings protect the floorpan the front window and the workplate segments To remove the transport security lock and the protective packagings please refer to the enclosed installation instructions and to Sections 5 2 and 5 3 of these instructions 11 12 Operating Instructions Biological Safety Cabinet MSC Advantage 3 1 Ambient conditions The operational safety and correct function of the device depends on the location where it is to be operated The biological safety cabinet must be operated only at locations that meet the ambient conditions listed below Location requirements The electrical system of the device has been designed for an operating height of up to 2000 m above sea level The mains power supply outlet should be out of casual reach to prevent ac cidental shut off Ideally the outlet should be installed above the safety cabinet The outlet must be accessible to authorized personnel only It constitutes together with the power cable plug the disconnection device for all poles The flooring of the location must be adequately strong and not flammable The stand must ensure a sufficient load bearing capacity twice the device weight The room in which the device is installed must be of adequate height For devices that are not connected to an exhaust system the distance between the exhaust air opening at the device ceiling and the room ceiling must be at least
29. f the footrest should be 45 x 35 cm C The slope should be adjustable within a range of 5 to 15 D The adjustable height should extend to a minimum of 11 cm above the floor After finishing an operation e Remove samples from the sample chamber and store them B properly e Clean and disinfect the sample chamber surfaces inclu ding the workplate and the floorpan Clean and disinfect all accessories Fig 15 Sitting posture 50128856_04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage 8 Shut down 8 1 Interrupting an operation To interrupt a work process 1 Remove all samples from the biological safety cabinet and store them properly 2 Remove accessories from the sample chamber and clean and disinfect them 3 Clean and disinfect the sample chamber surfaces the workplate and the floorpan 4 Switch the device to standby mode Close the front window and keep the ON key depressed until the indicators are off the right indicator segment of the display shows a dot to indicate that voltage is present Co NOTE Blower switch off For safety reasons the blowers can be swit ched off only when the front window is closed 8 2 Shutting the device down If the device is not to be used or stored for an extended period of time it must be completely decontaminated WARNING Decontamination measures To shut the device down the sample chamber must be disinfected completely
30. filters 5 and 2 for the device downflow and exhaust air HEPA filters Room air 10 is drawn into the sample cham ber through the working opening In the air duct room air and the downflow within the chamber 7 are then blended to make up the blend air 8 The blend air is then e filtered proportionally by the downflow filter 5 and supp lied as ultrapure air 6 evenly into the sample chamber of oo o the device e filtered by the exhaust air filter 2 and released as ultrapure air 1 to the exterior of the device Inlet air protection Multi segment protective grids 9 are in Stalled below the work surface in the air duct between sample chamber and device plenum The grids prevent coarse particles from entering the plenum where they might impair the function of the blowers 3 4 and of the filters 2 5 The grids can be removed for cleaning Fig 4 Filter system with downflow and exhaust air filter 20 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 4 Description of the device 4 4 Operating and display elements Fig 5 The biological safety cabinet is operated using an operating panel consisting of keys function indicators and a display A The display with its 5 digit indicator panel shows the following information re gardless of the activated operating function normal operation operating hours of the device safe work mode downflow and exhaust air velocities
31. hows dIS and the remaining disinfection time 31 32 Operating Instructions Biological Safety Cabinet MSC Advantage hermo Handling and control 6 1 4 Failure messages Failure messages are shown on the display as text number combinations with the codes ER 3 to ER 7 If one of these codes appears on the display contact Technical Service immediately 6 2 Device start up 1 Fig 14 Switch the device on keep the ON key 2 depressed until e the blowers start audible blower operation e the status indicators LEDs 9 12 illuminate Co NOTE Switch on A time delay of several seconds may occur between key actuation and device response 2 Move the front window to the work position The correct position is reached when the green status indicator FRONT WINDOW IS IN WORK POSITION 12 is illu minated The side guide rails have markings that define the lower edge of the front window these markings can be used as an additional orientation aid 3 Wait until the green status indicator AlRFLow Is sTEADyY 10 is illuminated 4 The device is ready for operation h Lro O O N SmartFlow O O O O O Fig 14 Display upon start up 50128856_04 2011 50128856 _04 2011 Operating Instructions Biological Safety Cabinet MSC Advantage 6 3 Description of the operating modes The following operating modes exist for the device e Calibration mode after initial start up e OFF mode e Window open
32. ings The standard fittings are 3 bushings per side panel SmartPort 6 is provided to lead through cables or hoses for accessories required in the utility chamber To this end the rubber grommet 5 is punched out in the exact opening size to avoid possible contamination Media valves 8 are installed solely in the bushings 7 Media valves suitable for installing in bushings can be supplied as an option Upon delivery of the device the bushings are sealed Fig 7 Supply interfaces 50128856_04 2011 50128856_04 2011 Operating Instructions Biological Safety Cabinet MSC Advantage AN CAUTION Unstable underpressure Do not switch the unit off as long as cables hoses are laid through the bushings If breached bushings are not used in operati on new covers must be inserted in the ope nings to ensure stable underpressure in the utility chamber see Chap 10 4 AN CAUTION Combustible gas If a gas burner is to be operated in the sample chamber an appropriate shut off device for the gas supply system shut off valve solenoid valve must be installed To ensure a safe distance to the recirculation filter the laboratory safety burner are to be placed at the working surface and not at an elevated position Use only laboratory safety burners in the sam ple chamber Potential equalization To avoid static charges and the associated risks if necessary intended supply connections and the device itself sh
33. lay shows the operating hours 2 Slide the front window up 9 3 2 UV disinfection using a mobile UV device optional To control the disinfection routine of a mobile UV device with the biological safety cabinet software ensure that the connecting plug of the UV device is compatible with the UV disinfection adapter of the biological safety cabinet To start the UV disinfection routine 1 Position the mobile UV device centered on the work area and connect it to the UV disinfection adapter 2 Close the front window completely the air system operates in the reduced mode yellow LED illuminated 3 Start UV disinfection using the UV key at the operating panel Keep the UV depressed until the display alternatingly shows dIS and the re maining disinfection time To interrupt cancel the UV disinfection routine 1 Press the UV key the display shows the operating hours 2 Slide the front window up 9 3 3 Changing UV disinfection time The UV disinfection time is preset this setting can be changed if required 1 Switch the cabinet on and move the front window to the work position 2 Keep the UV key depressed until the preset time is displayed 3 To increase the time Press the INTERNAL POWER SUPPLY key Each pressing of the key increases the time by 30 minutes 4 To reduce the time Press the INTEGRAL SAMPLE CHAMBER ILLUMINATION key Each pressing of the key reduces the time by 30 minutes 5 To save the values Press the
34. lting danger If the unit is tilted too much during lifting risk of tipping exists Lift the biological safety cabinet only vertically For transportation including inside buildings use an appropriate lifting device which ensures that the unit e is on a stable stand and e is secured against lateral tilting Do not transport the biological safety cabinet on a base with roles AN CAUTION Lift points For transport lift the device only at the lift points shown in Figure 2 Do not allow the weight of the cabinet to rest on the floorpan AN CAUTION Crushing hazard The weight of the front window is counterba lanced by the counterweight at the rear of the device The device must not be transported unless the counterweight has been locked e Install the four lockscrews see Section 5 3 Operating Instructions Biological Safety Cabinet MSC Advantage Fig 2 Lift points 15 16 Operating Instructions Biological Safety Cabinet MSC Advantage Description of the device 4 1 Overall view Fig 3 Plenum assembly 3 with plenum for downflow blower 19 and plenum for exhaust air blower 20 The downflow filter and the exhaust air filter are in stalled immediately to the pertaining plenum The exhaust air is discharged to the exterior of the device through an opening The plenum assembly is concealed behind a cover 18 Switchbox 2 with power supply unit and power supply cable 1 The top of th
35. m the device control starts an automatic calibration routine to deter mine the parameters for the safety system of the device C NOTE Calibration routine The calibration routine is no substitite for the installation check performed by the service personnel 5 2 Installing the device and accessories Device without stand e Place the device without stand onto a sufficiently stable substructure so that the weight of the device frame does not rest upon the floorpan e Remove the protective foil from the floorpan Device with stand Assemble the stand accessory and install the device frame onto the stand 1 Fig 10 Slide the two crossmembers 2 onto the retaining angles 3 of the side panels 1 then secure the crossmem bers to the side panels using the screws with wedge lock washers 4 2 To install the device frame 5 to the stand 7 insert four Allen screws with wedge lock washers 6 loosely into the threaded holes at the bottom of the device 3 Place the biological safety cabinet onto the stand so that the Allen screws with wedge lock washers 6 are routed through the holes 8 of the retaining tabs 10 4 Slide the device frame 5 into the retaining tab grooves 9 all the way to the stop 5 Tighten the four Allen screws with wedge lock washers 6 Operating Instructions Biological Safety Cabinet MSC Advantage Fig 10 Stand installation 25 26 Operating Instr
36. mode e Work mode e Standby mode e UV mode OFF mode Designates the idle state of the device e The device is switched off air system blowers are switched off e The sample chamber illumination is enabled e The internal power supply within the sample chamber is enabled If the internal power supply is activated the yellow status indicator INTERNAL POWER SUPPLY ACTIVATED is illuminated Window open mode Designates the state in which the window is open and positioned outside of the work position To install or remove auxiliaries the front window can be moved to the maximum opening position For cleaning the front window can be lowered see also Section 4 5 Fig 6 P 21 e The air system blowers are switched on The red status indicator AIRFLOW VOLUME STEADY S illuminated e The front window is not in the work position The red status indicator FRONT WINDOW NOT IN WORK POSITION S illumina ted e The sample chamber illumination is enabled e The internal power supply is enabled lf the internal power supply is activated the status indicator INTERNAL POWER SUPPLY ON S illuminated Work mode Ensures personal and material protection In this operating sta te the work process Is carried out in the sample chamber Work mode Is acti ve when the front window is in the work position and the air system is operating steadily e The front window is in the work position The green status indicator FRONT WINDOW
37. n of the biological safety cabinet the respective national regulations must be observed 90128856_04 2011 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 1 General notes 1 6 Explanation of symbols 1 6 1 Symbols used in the operating instructions AN GY y w WARNING is used if non observance may cause serious or even lethal injuries CAUTION is used if non observance may cause medium to minor injuries or damage NOTE is used for hints and useful information RECYCLING Valuable raw materials can be reused Warning against electric shock 90128856 04 2011 50128856 04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage 1 eT al e 1 male ats 1 6 2 Symbols on the device A N Observe operating instructions switchbox ceiling A Warning against hand injuries device side walls Biohazard left front section of device CE Conformity declaration Checked safety cover light dome Norme Francaise NF Postes de S curit Microbiologique 2 xT5A T5A note sample chamber fusing RS 232 interface top side of plenum panel Installion of the armrests right side of the light hood 10 Operating Instructions Biological Safety Cabinet MSC Advantage 1 7 Use of the device 1 7 1 Correct use The Biological Safety Cabinet is a laboratory device for installation and operation in microbi
38. ndicator FRONT winDow IN Work Position is illuminated and wait until the airflow has stabili zed the green status indicator AiRFLow sTEADyY is illuminated AN CAUTION Operational safety The personal and material protection is ensu red only if the airflow system of the device is working properly If the alarm system issues failure messages when the front window is in the work position stop all applications that may release harmful aerosols 4 Load the work surface with samples 5 For work intermissions or for extended experimental phases without manual in tervention switch the device to standby mode 7 3 Response to failure messages Failure messages are displayed in form of text number combinations with a code between ER 3 to ER 7 see Section 6 1 4 If one of these codes is displayed contact the Technical Service of Thermo Fisher Scientific immediately To isolate the cause of the failure the operating personnel must perform only the following tests and measures e Check to see if the exhaust air opening on top of the cabinet is blocked e Ensure that the on site exhaust air system is activated e Close doors and windows in the laboratory to prevent drafts e Switch off devices in the vicinity of the biological safety cabinet that cause air turbulence or emit excessive heat e Open flames in the sample chamber may impair airflow conditions Operating Instructions Biological Safety Cabinet MSC Advantage 35
39. nekanias 19 4 4 Operating and display elements ccccseeeseeeeeeeeeeseeeeeeeeeeeenaes 20 4 5 Sample chamber ACCESS cccccceeeeseccceeeeceeeeeeeeeeeeeeeeeeeeeeeeeeeseaas 21 4 6 Device interfaces ccccceecccccesseecseeseeeceeeeeecsauseecseseeessaseeessaaeees 22 4 7 Sample chamber illumination cccccccsseeeeecceeeeeeseeeeeeeeeeeeeeaes 24 4 8 UV lampunit ee en en mene en eee ee ere ee re 24 4 9 Working area csicctatersxccdudevaaeiatusncadaibeanentslantcadslinsteessiuntientdieneasiaversieeint 24 S SEUD a a enere eter 25 SI HPAII OD raO e E 25 5 2 Installing the device and accessories ssesceeceeseeeeaeeeeeeaeeeees 25 5 3 Unlocking the transport protection ccccceeeeeeeeeeeeeeeeeeeeeeeeeees 26 5 4 Levelling the cabinet ccccceeccccsssceeceeseeeceesseeeceseeessneeessasees 26 5 5 Power Supply CONNECTION cccccceseeeceeseeecceeseecceseeessseeessaeees 27 5 6 Installation test dinsccniierenaisesnuaeiiqnoswiiidiearsatinonaainidduass wit avanntiedencoiwnians 29 Handling and control ssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn mnnn 30 6 1 Operating panel cc ceeccccccccecceseeeeeeeeeeeeessaeeeeeeeeeeeeesaeaeeeeeeees 30 6 1 1 Functional UNIS seis cs ereneesrccones eeen EAREN 30 6 1 2 Display during calibration routine ccccccceseeeeeeeeeeeeeeeeeeens 31 6 1 3 Display during UV disinfection ccceeecceeeeteeeeeeeeeeeeeeeeee
40. ological and biotechnical laboratories of safety levels 1 2 and 3 It has been designed as a Class II microbiological biological safety cabinet in accordance with EN 12469 Depending on the hazard level of the agents involved the operator must prepare in writing appropriate decontamination procedures for the device and the acces sories used in the sample chamber Prior to the initial operation of the cabinet the operator must perform an installation test The test result must be documented by a test report The cabinet must only be released for operation if it is in compliance with the operating parameters spe cified by Thermo Fisher Scientific After any changes to the installation conditions and after any modification to the technical system a repeat test must be performed and the test result must be do cumented by a test report that shows that all operating parameters are in com pliance with those specified by Thermo Fisher Scientific 1 7 2 Incorrect use The biological safety cabinet must not be used in laboratories that do not comply with the requirements of safety levels 1 2 or 3 The device must not be operated as a Class II biological safety cabinet if e no repeat test is performed after changes to the installation conditions or after modifications to the technical system e the alarm system of the device has issued a failure message and the cause for the failure has not been repaired The alarm system must not be tampe
41. ons and important information are always accessible e Should you encounter problems that are not detailed adequately in these ope rating instructions please contact Thermo Fisher Scientific immediately for your own safety 50128856_04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage 1 eT al e 1 male E 1 4 Warranty Thermo Fisher Scientific warrants the operational safety and functions of the bi ological safety cabinet only under the condition that e the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions e the device is not modified e only original spare parts and accessories that have been approved by Thermo Fisher Scientific are used e inspections and maintenance are performed at the specified intervals e an installation test is performed prior to the initial operation of the device and that a repeat test is performed on the occasion of all inspections and repairs The warranty is valid from the date of delivery of the device to the operator 1 5 Standards and safety regulations The device complies with the safety requirements of the following standards and directives e EN 12469 e NF 095 Rev3 2006 NF Postes de S curit Microbiologique e IEC 61010 1 e EN61010 1 e EN 12469 e DIN EN 61326 1 e Low Voltage Directive 2006 95 EG e EMC Directive 2004 108 EG For the setting up and installatio
42. ors Fig 12 8 The air system is operated at reduced capacity the yellow LED is illuminated 9 Airflow is not steady the red LED is illuminated 10 Airflow is steady the green LED is illuminated 11 Front window is not in work position the red LED is illuminated 12 Front window is in work position the green LED is illuminated SmartFlow dispaly Fig 12 The SmartFlow Indicator displays the compensation capability of the exhaust con trol with window in working position and blower on The LEDs that illuminate in pairs indicate the following 18 green 17 green sufficient compensation capability 16 yellow 15 yellow compensation capability is depleted 14 red 13 red window outside working position or air velocity outside alarm range Co NOTE Compensation capability If the display is steady yellow yellow there is a possibility that the filters of the device are fully loaded In this case the technical service should be informed 6 1 2 Display during calibration routine CS NOTE Calibration routine The calibration routine is started only upon the initial operation The routine run takes approximately 30 minutes During this time the diplay al ternatingly shows cal and the remaining time If the calibration routine cannot be started due to failures the display shows cal permanently 6 1 3 Display during UV disinfection If a UV lamp is installed the diplay alternatingly s
43. ould be integrated in the on site potential equalization For this purpose the potential equalization connection on the stand can be used The installation of utility connections to the built in fittings must be made with con sideration to the current national technical rules External alarm systems This alarm contact can be used for two different external alarm systems e Potential free contact valve for driving external exhaust air systems technical ventilation e Potential free contact monitor alarm for the connection to an external alarm system failure reporting system or gas supply solenoids alll CAUTION High voltage w The contact with live parts may result to a lethal electrical shock During work on elec trical equipment shut of the device and all poles disconnected Repairs to the device must be carried out only by trained and authorized expert personnel 23 24 Operating Instructions Biological Safety Cabinet MSC Advantage 4 7 Sample chamber illumination Fig 8 The sample chamber illumination unit 2 e one flourescent tube for model MSC 1 2 e two flourescent tubes for model MSC 0 9 1 5 1 8 is installed behind the light dome 3 4 8 UV lamp unit Fig 8 The optional device integral UV lamp 1 is installed to the ceiling at the front section of the sample chamber The operating time of the UV lamp is preset The UV disinfection routine can be started by depressing a key on the oper
44. ower supply is ON the yellow status indicator INTERNAL POWER SUPPLY ACTIVATED is illuminated The biological safety cabinet can be switched off in OFF mode Keep the ON key depressed until all indicators are off UV mode For running the UV disinfection routine the front window is completely lowered to protect against UV radiation The routine cannot be run until the front window is in the closed position The UV disinfection routine is activated The yellow status indicator UV DISINFECTION ROUTINE ACTIVATED is illu minated until the preset time for the routine has elapsed Then the UV lamps are switched off automatically and the status indicator is swit ched off The sample chamber illumination is disabled The internal power supply in the sample chamber is disabled The integral UV lamp optional is enabled The power supply for the UV disinfection adapter optional is enabled 90128856_04 2011 50128856 _04 2011 7 1 Hygiene preparations for the sample chamber The sample chamber surfaces and the accessories required for the work process must be disinfected and cleaned in accordance with the hygiene guidelines set forth for the application 7 2 Loading the sample chamber Installing auxiliaries 1 Move the front window to the maximum opening position and switch the blo wers on 2 Install auxiliaries within the workplate working range 3 Move the front window to the work position the green status i
45. perforated plate must be installed e Checking the airflow conditions e Repeat test in accordance with EN 12469 2000 10 2 Service HEPA filters As the filter replacement is an interference with the safety system of the device filters must only be replaced by Thermo Fisher Scientific or by adequately trained and authorized service personnel WARNING Filter replacement For any service work in a potentially contami nated section of the biological safety cabinet a declaration of non objection by the manuf acturer must be presented 43 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 10 2 1 Sample chamber illumination Fig 18 The sample chamber is illuminated by one or two flu orescent tubes model MSC 0 9 1 2 1 5 1 8 The spring loa ded sockets of the fluorescent tube 2 are installed to the light dome mounting frame in the sample chamber 1 Switch the device off disconnect it from the power supp ly system and protect it against accidental reconnection 2 Move the front window down all the way beyond the frame SmartClean position to ensure a sufficient gap between window upper edge and light dome 3 The fluorescent tubes are retained by rotatable sockets Rotate the tube carefully by 90 into the removal position and remove it from the socket 4 Insert the new tube und rotate it into the contact position 10 2 2 Optional UV lamps Fig 17 The optional device integral
46. quest a written test report from the authorized test service ce NOTE Safety warranty The operational safety of the device particu larly the personal and material protection are guaranteed only if all safety functions of the device have been tested and approved Thermo Fisher Scientific will not warrant the operational safety if the device is operated wi thout performance of the required installation test or if the installation test and repeat test are not performed by adequately trained and au thorized personnel Co NOTE Device hygiene The initial operation with subsequent installa tion test does not include any decontamination measures For operation in the work process the sample chamber of the device and the ac cessories required for the work process must be disinfected and cleaned in accordance with the hygiene guidelines set forth for the application 29 30 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 6 1 Operating panel Fig 12 The operating panel is the major display and operating element and has been divided into three functional units e Display 1 keys 2 7 status indicators 8 18 6 1 1 Functional units Display Fig 12 1 Display for numbers and text e normal operation operating hours of the device e safe work mode downflow and exhaust air velocities e calibration routine status of the calibration function e device controlled UV di
47. r compressive force Installation of the power supply connection To protect from accidental switch off the power supply outlets should be loca ted out of casual reach und must be accessible only to authorized persons Ideally the outlet should be located above the biological safety cabinet The power supply cable plug is the interruption device in all poles Connecting the equipotential bonding If the device sample chamber is supplied with media gas water etc the on site equipotential bonding must be connected to the main ground of the device see service instructions Initialization routine Upon connection to the power supply system the device control passes through an initialization routine and switches the device to OFF mode the right indicator segment of the display shows a dot to indicate that voltage is present The biological safety cabinet is now ready for operation and can be operated using the keys Initial start up After the initialization routine the calibration routine is started after the first con nection to the power supply system e The display alternatingly shows CAL and the remaining routine time The entire calibration run takes approximately 30 minutes Operating Instructions Biological Safety Cabinet MSC Advantage 27 Operating Instructions Biological Safety Cabinet MSC Advantage hermo e The routine starts automatically when the front window is in the work posi tion and if
48. red with or disabled If alarm system com ponents heve been removed or disabled for service or repairs the device must only be released for operation if all alarm system components are functioning properly again The filters installed in the device are not capable of separating gaseous sub stances Therefore never store or process gases or gas releasing substances in the device e which in quantity or concentration are toxic e if a reaction with other substances may result in hazardous toxic concentrations or formation of toxic gases e that may form combustible or explosive mixtures in combination with air 50128856_04 2011 90128856_04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage 2 Delivery 2 1 Scope of delivery Delivery for the biological safety cabinet includes the following e biological safety cabinet without stand e armrests e device documentation operating instructions factory test report Optional components and accessories are listed as separate items in the deli very document 2 2 Acceptance inspection After the device has been delivered immediately check the device e for completeness e for possible damage If the delivery is incomplete or if you detect any transport damage to the device contact the forwarding agency and Thermo Fisher Scientific im mediately 2 3 Transport security lock and device packaging A transport security lock protects the d
49. rkplate and adjust the four stands until the level indi cates an absolutely hotizontal position in all directions Proceed the elevating adjustment from right to left and from rear to front Fig 11 Unlocking the transport protection 50128856 _04 2011 5 5 Power supply connection WARNING High voltage 2 m Contact with current carrying components may cause a lethal electric shock w Before connecting the device to the power sup ply system check plug and power supply cable for possible damage Do not use damaged components to connect the device to the power supply system Establishing the power supply connection 1 Before connecting the device to the power supply system check to see if the voltage of the outlet corresponds with the specifications on the nameplate of the device If the ratings given for voltage V and maximum current A do not match the device must not be connected to the power supply system 2 Connect the grounding plug of the device to a properly grounded and fused outlet e The outlet must be fused separately using a fusible link T 16 Aor using a circuit breaker B 16 3 Make sure that the power cable is routed away from the counterweight and cable guide For this purpose the power cable can be secured to the device ceiling using the enclosed adhesive bases and cable ties see installation instructions 4 Make sure that the power supply line is not subjected to tensile o
50. shing agent Then wipe the exterior surfaces dry using a soft clean cloth 9 6 Cleaning the front window For cleaning and disinfection using the wipe disinfection procedure the front window can be lowered beyond the closing position see Section 4 5 The gap between the light dome and the front window upper edge ensures that the upper section of the window can be cleaned or disinfected Clean the front window using a commercially available window cleaner Operating Instructions Biological Safety Cabinet MSC Advantage 41 42 Operating Instructions Biological Safety Cabinet MSC Advantage Cleaning and decontamination 9 7 Cleaning the floorpan Clean the floorpan using a solution of tepid water and commer cially available dishwashing agent 1 2 3 5 Remove the workplates from the sample chamber Remove dirt residues and deposits thoroughly Wipe the floorpan clean using a clean cloth and plenty of clear water Remove the cleaning liquid from the floorpan and wipe the floorpan surfaces thoroughly clean Ce NOTE Material residues After cleaning make sure that all cleaning materials have been removed completely from the floorpan Reinstall the workplates 9 8 Cleaning the protective grid Fig 16 The multi segment protective grid is retained in the installed position between support surface and airduct wall by its own tension 1 To remove a grid segment 2 push the retaining tab 1
51. sinfection optional remaining disinfection time Keys Fig 12 The keys are used for enabling disabling device functions 2 Key for switching the device on off switch off only in standby mode see Section 6 3 3 Operating data key with dual function e Indicating operating data Press the key to switch between the data Operating hours downflow and exhaust air velocities in the safe work mode e Enabling I disabling 0 the alarm acknowledge function In the OFF mode keep the key depressed for approx 5 second The display momentarily shows the operating hours then the switching state alarm acknowledge function enabled 0 alarm acknowledge function disabled Within the scope of the EU EN12469 2000 the alarm acknowledge function is disabled upon delivery of the device 4 Key for switching the sample chamber illumination on and off 5 Key for switching the power supply of device integral outlets on and off the LED indicates that voltage is present 6 Key for switching UV disinfection on and off the yellow LED indicates that the UV disinfection routine has been activated if the optional UV lamp is not installed this key has no function 7 Key for acknowledging the audible alarm 1 3 h m s SmartFlow Fig 12 Operating panel 50128856_04 2011 90128856 _04 2011 hermo Operating Instructions Biological Safety Cabinet MSC Advantage 6 Handling and control Status indicat
52. t interfere with the plant traffic a Fig 1 This figure shows preferred locations for biological safety m I cabinets and unsuitable locations that are not in accordance with the safety requirements Unsuitable locations The locations 1 2 and 3 are not KY Suitable because they are exposed to drafts from windows and doors Location 5 is unsuitable because it is within range of plant traffic and within the exhaust air range of a ventilation system 4 Preferred locations The locations 6 7 and 8 are correct because they are in a draft free section of the room and not ex posed to plant traffic A counterweight at the device backpanel moves synchronously with the vertical movement of the front window To prevent the counterweight from jamming the device backpanel should be ET to i re 7 X O TT Ne 0 la oJ as close to the wall as possible 3 4 Installation in series 8 When several devices are to be installed in series please ob Fig 1 serve the following Device arrangement in the e Make sure that vibrations cannot be transferred operating room between adjacent devices e Exterior surfaces of the cabinets must always be ac cessible for cleaning and disinfection 90128856_04 2011 3 5 Transport Fig 2 To prevent tilting always transport the device using a sui table carrier even for a transport within a building and separate it from the stand see Section 5 2 AN CAUTION Ti
53. taminated particles escaping from the utility chamber the unit must be switched on in order to exchange the SmartPorts The SmartPorts are removed inwards into the utility chamber 1 First remove the inner grommet of the SmartPort in the utility chamber inwards from the side panel opening 2 Then remove the outer grommet of the SmartPort in the utility chamber inwards from the side panel opening 3 Disinfect both grommets in the utility chamber and take them out of the utility chamber 4 Insert new outer grommet in the side panel opening from the outside 5 Insert new inner grommet in the side panel opening from the inside 45 46 Operating Instructions Biological Safety Cabinet MSC Advantage hermo 11 1 Disposal procedure Discarded cabinets or device components contain reusable materials All com ponents with the exception of the HEPA filters can be discarded after having been thoroughly cleaned and disinfected The HEPA filters must be discarded in ac cordance with the applicable national and state regulations for special solid waste AN CAUTION Contamination hazard As the device can be used for processing and treating infectious substances it may be contaminated Prior to disposal the complete device with filters must be decontaminated by performing a formaldehyde sterilization LKY ex 9 Recyclable materials Comp oneni T S plastics mounted on epoxy resin bound boards Note material labeling
54. that a sufficiently high gap exists between the sample chamber ceiling and the front win dow upper edge This gap can be also used for replacing the sample chamber illumination lamps AN CAUTION Front window jamming Do not use force to move the front window If the window is jammed or sluggish contact Technical Service immediately Do not attempt to repair this problem Fig 6 Sample chamber opening 21 22 Operating Instructions Biological Safety Cabinet MSC Advantage 4 6 Device interfaces Fig 7 The standard equipment includes e outlets for internal external power supply e bushings on both sides for cables media valves and hoses e acommunication port e aconnection to external alarm systems Power supply connection The connection to the power supply system is es tablished via a cable with grounding plug 4 at the upper side of the device Device integral power supply The backpanel contains outlets 10 for the power supply overall maximal current 5 A of internal accessories e 2 outlets model MSC 0 9 1 2 e 4 outlets model MSC 1 5 1 8 Optionally an outlet can be replaced with a disinfection adapter 9 for the con nection of a mobile UV device Two fuse holders for 5 A miniature fuses at the top of the front cover protect the device integral power supply 1 for L 2 for N Communication port The top of the device side contains an RS232 connec tion 3 for a PC Bush
55. the air flows freely and steadily If the airflow is disturbed during this run time the process is cancelled and the calibration routine is restar ted e To complete the routine the determined parameters for safe operation and the alarm limits are stored automatically C NOTE Initial operation According to applicable national standards and regulations the calibration routine is no substitute for a start up performed by an au thorized service technician 50128856_04 2011 50128856_04 2011 Operating Instructions Biological Safety Cabinet MSC Advantage 5 6 Installation test Do not operate the device before an initial operation with installation test has been performed after the final installation of the device e The installation test must be performed in accordance with the specificati ons of EN 12469 2000 The cabinet may be operated as a Class II micro biological biological safety cabinet in accordance with EN 12469 2000 if the device functions or function patterns listed below were checked and if the test results are within the safety value tolerances specified by the manufacturer in Annex F e Electrical safety test e Inflow velocity test e Downflow velocity test e HEPA filter leakage test e Airflow control test e Arepeat test must also be performed after repairs to the device or after consi derable changes more than 5 cm to the location of the device e The operator must prepare a test report or re
56. uctions Biological Safety Cabinet MSC Advantage 5 3 Unlocking the transport protection Fig 11 The front window counterweight 1 is secured to the device backpanel to protect it from transport damage AN CAUTION Unlocking the counterweight The counterweight at the device backpanel compensates for the weight of the front win dow The front window must not be moved unless e the counterweight has been unlocked so that it can move freely the protective packaging has been removed from the front window e the device frame has been installed safely on a sufficiently stable substructure or onto a stand Crushing hazard after unlocking keep hands and fingers away from the motion range of the counterweight To unlock the transport protection Remove the four retaining screws position see arrows Keep the counterweight retaining screws for later transports 5 4 Levelling the cabinet The cabinet should be levelled only after it has been positioned 1 2 Remove transport protection vinyl from the workplate or from the workplate Place the workplate onto the front and rear support rails in the sample chamber with the wide perforated track facing the front window Devices without stand Place a bubble level onto the work plate segments and align the stand until the level indicates an absolutely horizontal position in all directions Devices with optional stand Place a bubble level onto the wo
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