Class 1 OM Manual (2010) Rev B
Contents
1. 0111 A 2 HORIZONTAL 2 LAMINAR FLOW Position 1 An acceptable site not affected by disruptive air currents Position 2 Well sited Position 3 Poorly sited if windows open If not it should be a safe distance from the cabinet opposite Position 4 Poorly sited can be affected by air currents from the opening door through traffic and the horizontal laminar flow workstation sited directly opposite Installation 1 4 Safety Cabinets are sophisticated items of equipment containing delicate filters which require expertise in their safe handling and installation into laboratories For exhaust type safety cabinets the exhaust ductwork route should ideally be surveyed and ductwork installation be carried out by qualified engineers as it forms an integral part of the system relating to the overall performance of the cabinet and is required to conform to various safety standards A poorly installed cabinet may compromise the protection provided to both personnel and work being handled and may present a hazard to other occupants of the building and the public e Make up Air It is important that any make up air compensating for the air exhausted from the safety cabinet does not cause draughts to the discomfort of the laboratory staff or detriment of the cabinet per2. 4 3 The HEPA filter is located on the top of the main body of the cabinet held in place by four M8 filter bolts IMPORTANT Cabinet must be decontaminated prior to changing any HEPA filters see section 2 6 Only replace or examine filters if authorised to do so by the Safety Officer or the person in charge of the laboratory To remove any filters wear disposable gloves an apron overalls and appropriate face covering especially eye protection Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels Warning To remove filter release bolts lift module until filter can be removed Slide the filter forward and out and dispose of as recommended by site regulations To replace first ensure that all surfaces have been cleaned and are free from matter Remove new filter from its protective packing Inspect both exposed surfaces for signs of damage Any damage should be reported to the supplier immediately Slide filter carefully into position Tighten bolts ensuring filter is correctly positioned and gaskets are compressed to about half their original thickness The filter and its gaskets should be tested for leaks by challenging with DOP or equivalent aerosol and scanning the surface with the probe of a forward light scattering photometer General Notes 4 4 gt Do not store equipment inside the Microbiological Safety Cabinet The amount of equipment should be kep
3. Fumigation Procedure 2 6 gt Switch off the cabinet fans by pressing the green button A on the control panel gt For re circulating type safety cabinets the fumigation extract kit optional should be fitted to the cabinet discharge and ensure that the manual damper is fully closed For cabinets connected to a duct system proceed as follows Fill the formalin vaporiser with the correct amount of Formalin see section 2 5 for quantities and screw on the aluminium cap finger tight having checked the gasket in the cap is undamaged If the vaporiser is free standing place on the cabinet work tray plug into cabinet internal socket and switch on Now fit the closure panel night door if using a lightweight closure panel additional sealing tape will be required around the outside face to ensure no leakage during fumigation Now press the fumigation button E and the display will show FUMIGATION IN PROGRESS A countdown timer will be shown directly below in minutes e g 285 MINS Ensure all laboratory personnel are aware that the fumigation is taking place appropriate warning notices should be put on all doors entering the laboratory and on the cabinet being fumigated Warning The cabinet should be left for a minimum of 6 hours preferably overnight Note Once the fumigate button has been activated DO NOT SWITCH CABINET ON until the cycle has been completed On completion the
4. Hectricat Data Typical LLL AC Internal Socket s 230v 13Amp 230v 13Amp 230v 13Amp 230v 13Amp H14 EN1822 H14 EN1822 H14 EN1822 H14 EN1822 99 999 99 999 99 999 99 999 Your Manual 1 7 This user manual has been prepared to provide a basic operating and maintenance instruction It is intended to supplement existing in house procedures and codes of practice and not to replace them If further advice is required on the use or maintenance of this equipment the staff of Contained Air Solutions Ltd will be pleased to assist wherever possible Switches amp Indicators 2 1 The diagram below shows a typical control panel membrane layout for the BioMAT 1 Safety Cabinet A Cabinet Operation B Lights C Gas Valve Optional D Alarm Mute F UV Lights Optional G Key Switch EN Starts Stops power supply to fans and control circuits Controlling power to lights To activate solenoid Gas Valve Will only function when cabinet is in safe working condition To mute audible alarm Fault indication on display will remain until fault is rectified To activate fumigation cycle Will only operate when cabinet is switched OFF To activate power to UV Lights Will only operate when cabinet and lights are switched OFF Key switch for supervisor control used to prevent cabinet operation during fumigation Start Up Procedure 2 2 The follow
5. body of the cabinet separated by a spacer section and held in place by four M8 filter bolts IMPORTANT Cabinet must be decontaminated prior to changing any HEPA filters see section 2 6 Only replace or examine filters if authorised to do so by the Safety Officer or the person in charge of the laboratory To remove any filters wear disposable gloves an apron overalls and appropriate face covering especially eye protection Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels Warning To remove filters release bolts lift module until filters and spacer section can be removed Slide one filter forward and out and dispose of as recommended by site regulations then remove second filter in same manner To replace first ensure that all surfaces have been cleaned and are free from matter Remove new filters from their protective packing Inspect all exposed surfaces for signs of damage Any damage should be reported to the supplier immediately Slide filters and spacer carefully into position Tighten filter bolts ensuring filters and spacer section are correctly positioned and gaskets are compressed to about half their original thickness The filters and their gaskets should be tested for leaks by challenging with DOP or equivalent aerosol and scanning the surfaces with the probe of a forward light scattering photometer Exhaust HEPA Filter Air ducted to atmosphere
6. cabinet will display FUMIGATION COMPLETE and READY TO VENT When ready press the fumigation button to exit the cycle and then press the green button A to purge the cabinet of formaldehyde gas For re circulating type safety cabinets press the fumigate button to complete cycle and open the manual shut off damper on the fumigation adaptor kit Optional having first fitted the extract tubing and placed the discharge point in a location approved by the Laboratory Safety Officer When fumigating a re circulating type safety cabinet where venting is unobtainable a mobile carbon filter unit may be required gt Switch on the cabinet and then remove the sealing tape To avoid formaldehyde being drawn out of the cabinet remove the rubber bung when using a 100 sealing closure panel gt Within the first few minutes of purging the majority of the formaldehyde gas within the safety cabinet will be removed However due to the fact that formaldehyde adheres to the surfaces of the cabinet and within the media of HEPA filters we recommend that the cabinet be run for between 4 and 6 hours before the cabinet is serviced or work re commences Remove the optional fumigation kit from the cabinet discharge before commencing to use the cabinet Any poly formaldehyde residue in the vaporiser may be removed by heating with water containing a little mild detergent at neutral pH General Note Other methods of generating form
7. VISOR OPEN and an audible alarm will sound This can be muted using the alarm mute button The alarm circuit is activated each time the cabinet is switched on It is important to ensure that the pressure tubing is not damaged or split where it is connected to the pressure sensor The pressure sensor in the event of inadequate airflow in either part of the system will cause the alarm circuit to be activated with a flashing red indicator on the alarm mute button an intermittent audible alarm tone and the alarm system will clearly show on the display the fault The audible alarm tone can be silenced by depressing the alarm mute button on the control panel after which the red light will remain continuously lit until the correct airflow is reinstated or the cabinet is switched off Electrical Protection 3 3 Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels Warning Fuses There are 11 fuses mounted on the printed circuit board to protect the electrical circuits of the cabinet The cabinet controls are housed behind the front control panel which is secured with two screws located across the bottom edge Once removed the control panel will hinge upwards to facilitate access to the main printed circuit board For full details on fuse ratings and the circuits they protect please see the wiring diagram attached to this manual Fan Speed Control 3 4 The inflow fans ca
8. aldehyde and other methods of cabinet decontamination can be employed prior to using alternative methods your Laboratory Safety Officer should be consulted Contained Air Solutions will be pleased to advise if they are able but a detailed knowledge of every technique cannot be guaranteed If your cabinet is fitted with ports to allow fumigation using Vaporised Hydrogen Peroxide VHP please refer to the VHP equipment manufacturer s manual for the correct application of this technique Airflow Measurements 2 7 It is recommended that the inflow airflow be checked regularly i e weekly or monthly according to cabinet usage A rotating vane type anemometer with a head diameter of about 100mm is considered satisfactory On 1200mm wide cabinets take 5 readings namely one in each corner and one in the centre on the work area aperture Readings should be not less than 0 70m sec or greater than 1 0m sec Readings above 1 0m sec may cause problems with containment this may be checked by conducting a KI test Anemometer positions for the 1200 1500 amp 1800mm size of cabinets are illustrated below The results should be recorded to monitor cabinet performance over time ep 1200mm BioMAT 1 ceo Pp p 1500 amp 1800mm BioMAT 1 Ultraviolet Radiation Optional 2 8 Ultraviolet Radiation UV lamps may be fitted as an optional extra these can be fitted as new in our factory or retrofitted at a later date on site If instal
9. cation repairs or other maintenance is carried out by CAS personnel or persons authorised by CAS or in the case of electrical work by qualified electricians gt In the case of repair or maintenance replacement parts supplied by CAS must be used gt The electrical installation surrounding the unit and to which it is connected comply with the latest IEC regulations The unit is used and maintained in compliance with the instructions contained in this manual CE Declaration of Conformity CAS declares that the equipment supplied conforms to the following CE directives Machinery 2006 42 EC Electro Magnetic Compatibility 2004 108 EC Low Voltage 2006 95 EC Cabinet Siting 1 3 The siting of your Safety Cabinet is extremely important Air currents and the movement of people in the laboratory can adversely affect the performance Safety Cabinets should be sited away from gt gt gt gt gt Doors and windows which open Draughts caused by ventilation and air conditioning units Pedestrian traffic routes Other safety cabinets or fume cupboards Adjacent fridge amp Incubator doors These points are of particular relevance to class 2 safety cabinets The diagram shown below indicates some suggested locations for the correct siting of safety cabinets and highlights some situations which should be avoided g B O O
10. ered via high efficiency filtration HEPA prior to being dispersed back into the laboratory or to atmosphere Operator protection is provided by a constant inflow of air through the working aperture of the front screen The Class 1 Microbiological Safety Cabinet can be supplied as standard in either of the following two modes gt Re circulating Type Exhaust air from the safety cabinet is passed through two high efficiency filters HEPA before being released back into the laboratory The exhaust air is made up of 100 of the total air volume handled by the safety cabinet gt Exhaust Type Exhaust air from the safety cabinet is passed through a high efficiency filter HEPA before being extracted to atmosphere through a fan assisted extract system The exhaust air is made up of 100 of the total air volume handled by the safety cabinet Quality Assurance 1 2 Although fully tested before leaving our factory as part of the ISO 9001 2008 Quality Assurance Programme the specified performance will only be maintained if your cabinet is sited correctly and regularly serviced CAS can only accept responsibility for correct functioning of your cabinet if gt The Safety Cabinet is correctly sited in the laboratory to avoid any adverse conditions within the room that may affect the level of operator protection gt It has been installed and commissioned by CAS trained personnel or approved CAS agents Extension modification relo
11. et performance e e 300 1500 Keep pedestrians away from the front Keep clear of adjacent wall Position well away from the door openings of your safety cabinet e 1500 2000 e Bench lt gt Position clear of bench opposite Keep clear of structural columns Keep well away from opposite wal Technical Data 1 6 w d h 900 630 1250 1200 630 1250 1500 630 1350 1800 630 1350 w d h 895 625 700 1195 625 700 1495 625 700 1795 625 700 Opening to Work Ares to Work Area w h 710 200 1010 200 1310 200 1610 200 Weight Typical FWeight Typical Kg 150 20 250 30 Loading Capacity woading Capacity E E Work Surface ie E me Ee Air Volumes Typical Exhaust Air Volume sec Re circ Air Volume sec ie E mise Pressure Drop Typical Exhaust Clean Filters Pa es T mE fu aou 99 999 7 Heat cains Tyi 5 5 UI eo Exhaust Dirty Filters HEPA Filter Data HEPA Type i iL Heat Gains Typical M NOISE Typical E Light Typical Lighting Level White Lighting Level St St ane Ve 0 Duct Connection Duct Connection Exh M
12. formance Air supply diffusers should be positioned more than 1500mm away from the front of the safety cabinet and have a maximum velocity of no more than 0 30m sec e Commissioning When any safety cabinet is installed it is necessary to carry out a number of commissioning checks in order to ensure it is fully operational and that the performance on site satisfies the current standard BS EN 12469 2000 This includes measuring the airflows testing the HEPA filters with a suitable challenge aerosol and a KI Discus Test operator protection test to assess the containment of the cabinet CAS employs a team of fully trained installation and commission engineers to carry out all work necessary This ensures that all new safety cabinets operate to the desired performance e Site Surveys If you have any queries regarding the siting of your safety cabinets we will be only too pleased to arrange a site survey by one of our regionally based technical support staff e Periodic Maintenance amp Servicing To maintain safety cabinets at their optimum level of performance and to ensure lifetime operation regular servicing is necessary CAS provides a full servicing and maintenance scheme tailored to suit your individual needs For more information on this please contact our service department on 0161 655 8860 Avoiding Disturbances 1 5 The sketches below show recommendations for avoiding disturbances for both cabinet operator and safety cabin
13. ight door over the working aperture Cleaning Procedure 2 4 Regular cleaning is very important to prevent the build up of dirt and hence potentially infectious material Routine swabbing of work surfaces with 70 v v IMS ethanol or IPA Isopropyl Alcohol is recommended For cleaning the work surfaces swabbing with a mild detergent in warm water is very effective Phenolic or Cresolic disinfectants should be avoided as they may stain the white surfaces with a brownish colour If they are used any spillage should be quickly rinsed with clean water and mopped up with an absorbent tissue Most of the quaternary ammonium compounds and the Glutaraldehyde based surface disinfectants are suitable To facilitate cleaning of the work zone and the interior the whole front screen visor may be opened and lowered from the top supported by the hinges at the lower edge It is good practice to clean the inside of the viewing screen to ensure adequate visibility of the working zone Always consult the Laboratory Safety Officer before carrying out this procedure If Hypochlorites are used to clean the stainless steel interior of the safety cabinet they will initially cause rust spots and over time may lead to further damage Warning Fumigation amp Formalin Quantities 2 5 When handling hazardous materials the air space inside the cabinet should be decontaminated regularly and always before servicing and following any spillage
14. ing notes are for guidance where local laboratory instructions do not exist or are inappropriate They should complement not replace existing codes of practice issued by your Laboratory Safety Officers gt Ensure power supply to the cabinet is switched on as evidenced by the main display being illuminated The key switch on the front of the cabinet must be turned to position 1 ON gt Press the green switch A on the control panel this will energise the exhaust fan The main display will show AIRFLOW STABILISING for 60 seconds once airflows are settled the display will show AIRFLOW SAFE On start up the audible alarm will sound until airflows are safe this may be muted using the Alarm Mute switch D NOTE Should the airflows change significantly and fall out of the specifications laid down in EN12469 2000 the cabinet alarms will be automatically activated gt Switch on the interior lighting using switch B Lighting can be activated when the cabinet is OFF this can be used if loading or un loading the cabinet with equipment prior to use Shut Down Procedure 2 3 The work area should be cleared of any apparatus equipment and cleaned in accordance with laboratory codes of practice The cabinet should be left running for a few minutes to clear any residual aerosols Switch off the interior light using switch B Switch off the cabinet using switch A Place the closure panel N
15. led the cabinet will have1 or 2 Depending on cabinet size short wavelength Ultraviolet UV tubes emitting 254 nano metres fitted to the inside work area of the safety cabinet As a safety feature the UV tubes are interlinked with the cabinet lights to prevent them being used when the cabinet is in use Over time the effective life of UV tubes is known to reduce therefore we would recommend UV tubes are replaced on an annual basis to ensure maximum efficiency Applications Many bacteria are quite resistant to UV Radiation and may require prolonged exposure for sterilisation Dry and or protein covered organisms may be protected against UV and may be only slightly affected if at all However moist vegetative cells without too much protein covering are killed with reasonable effectiveness after 3 4 hours exposure Extreme care must be exercised when using UV radiation Consult your Laboratory Safety Officer prior to use Warning UV Radiation can cause burns to unprotected skin and it is very important not to look directly at the illuminated tubes with the naked eye Display 3 1 The cabinet display is located in the centre of the control membrane it incorporates backlighting to ensure all signs can be clearly seen from the operating position Typical display during normal operation shown below In the event of an alarm condition the display will clearly indicate the fault this will remain displayed until fau
16. lt is rectified Typical display during alarm condition shown below Should the cabinet produce an alarm condition it may be necessary to arrange for a Service Engineer to attend site in such cases please contact our Service Department on 0161 655 6183 Alarm Circuits 3 2 There are three standard alarm circuits on the BioMAT 1 Re circulating and Exhaust type Safety Cabinets 1 INFLOW LOW ALARM Sensed by an accurate pressure sensor mounted on the main printed circuit board and connected via tubing to the sensing points within the cabinet body this will sense low airflow caused by fan failure or filter soiling and transmit the signal to the display meter on the front panel and alarm circuit In the event the alarm is triggered the display will show LOW AIRFLOW and the audible alarm will sound this can be muted using the alarm mute button 2 INFLOW HIGH ALARM Sensed by an accurate pressure sensor mounted on the main printed circuit board and connected via tubing to the sensing points within the cabinet body this will sense high airflow and transmit the signal to the display meter on the front panel and alarm circuit In the event the alarm is triggered the display will show HIGH AIRFLOW and the audible alarm will sound this can be muted using the alarm mute button 3 FRONT SCREEN Front screen position is monitored by a micro switch fitted to the front screen assembly When opened the display will show
17. n be adjusted via the engineer s menu please see section 3 5 for more information To maintain optimum performance during routine planned maintenance it may be necessary to increase cabinet fan speeds to overcome filter soiling This can be adjusted via the front control panel membrane using the Engineer s Menu It is important any changes to fan speeds must be made by a CAS service engineer or alternative competent service provider failure to do this may result in the warranty being invalid Warning Engineer s Menu 3 5 It is important any changes within the Engineer s Menu be conducted by a CAS service engineer or alternative competent service provider failure to do so may result in the warranty being invalid Warning The buttons located on the front control membrane have a second function once access to the Engineer s Menu has been gained In order to gain access to the Engineer s Menu the cabinet must be switched OFF STANDARD SWITCH PANEL ARRANGEMENT SOFT BUTTON ARRANGEMENT TO ENTER ENGINEERS MENU BUTTON 1 SHOULD HELD DOWN FOR gt 5 SECONDS TO EXIT ENGINEERS MENU BUTTON 1 SHOULD HELD DOWN FOR gt 5 SECONDS USE BUTTON 2 TO START STOP THE FANS Engineer s Menu Sequence nan fA Ho EH H 8 x 8 Re circ HEPA Filter Air returned to room 4 2 Two HEPA filters are located on the top of the main
18. ontained Air Solutions BioMATeo 1 Safety Cabinet Section Section 1 Your Manual Section 2 Using your Cabinet Section 3 Cabinet Control Panel Section 4 Filter Replacement Section 6 Service amp Spares 4 1 4 2 4 3 Contained Air Solutions Description General Description Quality Assurance CE compatibility Cabinet Siting Installation Avoiding Disturbances Technical Data Your Manual Switches amp Indicators Start Up Procedure Shut Down Procedure Cleaning Procedure Fumigation amp Formalin Quantities Fumigation Procedure Airflow Measurements Ultraviolet Radiation Display Alarm Circuits Electrical Protection Fan Speed Control Engineers Menu Re circ HEPA Filters Exhaust HEPA Filter General Notes Service Schedule Spares List Drawings attached Wiring Diagrams attached General Description 1 1 Your BioMAT 1 Microbiological Safety Cabinet has been designed to provide optimum performance for operator protection Using the latest in microprocessor and fan technology it is designed to exceed the performance requirements of the European Microbiological Safety Cabinet Standard EN12469 2000 The safety cabinet carcass and all seals are maintained under negative pressure ensuring air cannot leak out during normal operation Class 1 Safety Cabinets offer a high level of operator protection they ensure any aerosols generated within the cabinet are filt
19. rm parameters amp control functions as required Check and adjust electrical and electronic controls as related to the airflow system Check and adjust operation of exhaust system as required Mechanically inspect cabinet components and duct connections if fitted Change main filters as necessary Supply and fitting subject to additional charge Prepare computer generated Service Report Above work to be carried out twice each service year Operator Protection Test K I Discus carried out once per year unless instructed otherwise Service schedules can be tailored to suit individual needs For a competitive Quotation please contact our service Department on 0161 655 8860 Spares List 6 2 Shown below are ordering codes for the most common parts used on the BioMAT 1 Safety Cabinets In addition to these items we stock a vast range of Spares consumables and optional extras If you cannot see what you require please give us a call on the telephone number shown below Please note cabinet serial number must be quoted when ordering UK Overseas Tel 0161 655 8860 Tel 44 161 655 8860 Fax 0161 655 8865 Fax 44 161 655 8865
20. s Fumigation by formaldehyde gas is the recommended decontamination procedure for biological hazards although there are alternative methods available including VHP decontamination and Ozone decontamination To facilitate the fumigation a sequence has been incorporated in the cabinet controls this is detailed under fumigation procedure A convenient way of generating sufficient formaldehyde is to boil off Formalin 40 formaldehyde BP or equivalent in a suitable vessel such as a formalin vaporiser These are available from CAS Always consult your Laboratory Safety Officer prior to fumigation of a safety cabinet If in doubt ask Formalin Quantities The recommended quantity stated in BS EN12469 2000 on Page 40 Annex J Part 2 is 60ml formaldehyde solution mixed with 60ml distilled water per cubic meter of cabinet volume However this quantity is now considered in excess of that required to achieve a satisfactory kill We have therefore produced the following table based on quantities employed by users of large numbers of Safety Cabinets If you still consider that the quantities recommended in the British Standard are to be used you may find on completion of the sterilisation cycle that high quantities of fluids containing formaldehyde are present in the cabinet JIT JU L 12 D L 1500m LOU 1 rmalin 15ml amp 40 20mI 40 25mi 40 30mlG40 Distilled Water 15ml 20m 25m 25m
21. t to a minimum to reduce the disruption to the airflow patterns within the cabinet gt A Bunsen burner should not be used inside the cabinet gt The failsafe solenoid operated gas valve If fitted will only allow gas to flow when the cabinet is switched on and there is a satisfactory airflow Any interruption in the power supply or failure of air supply necessitates manual resetting of the valve by depressing the switch on the control panel marked gas valve gt As a safety feature the UV tubes If fitted are interlinked with the cabinet lights to prevent them being used when the cabinet is in use We recommend cabinet users to consult their own laboratory safety information Other publications available include Science Reviews Ltd Occupational Hygiene Monograph No 9 British Standard BS EN 12469 2000 amp 5726 2000 Part 5 Australian Standard AS2567 American National Sanitation Foundation Standard No 49 We would also recommend the latest guidelines issued by the UK Advisory Committee on Dangerous Pathogens ACDP These documents contain much useful information on the performance installation testing use and limitations of Microbiological Safety Cabinets Schedule of work included in service agreement Filter Integrity Test D O P on all main filter s and seals Airflow Profiling ensuring compliance to latest British amp European Standards Operator Protection Testing K I Discus 1 x Per Annum Check and adjust ala
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