QE-1HC (090218) - Warner Instruments
Contents
1. mm glass bottom cell culture dishes The platform is at home on both upright and inverted microscopes and includes adjustable perfusion tubes magnetic quick release clamps and Peltier heating and cooling elements A complete system requires the addition of a temperature controller and heat exchanger thermal cooling module Temperature controllers and heat exchangers sold separately The QE 1HC is designed to be used with a CL 100 or CL 200 bipolar temperature controller and a TCM 1 thermal cooling module The temperature controllers provide feedback driven regulation of heating cooling power and the TCM 1 quietly and effectively dissipates the excess heat generated by the operation of the QE1 HC s integral Peltier device The QE 1HC base has magnetic stainless steel plates along the upper perimeter for use with magnetic attachments and a 25 mm diameter aperture for use with inverted microscope objectives Stage adapters are available for all major microscopes Removable magnetic perfusion and suction tubes T allow precise placement of perfusion lines into any dish or chamber The magnets facilitate simple repositioning of both suction and perfusion lines following chamber exchange A redesigned suction tube eliminates tidal action and fluid flutter in the bath for quiet aspiration Complementary in line temperature control of perfusate can be achieved using our SC 20 Dual In Line Solution Heater Cooler along with an associated tempera2. QE 1HC Rev 090218 Warner Instruments Quick Exchange Heated Cooled Platform for 35mm Petri Dishes Model QE 1HC Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 800 599 4203 203 776 0664 203 776 1278 fax J Warner Instruments QE 1HC Rev 090218 Table of Contents DE CRIP 8 Jy en rettrre ereeerrr O A rrr rrr rrr rrr err rr rrr rT rT 3 INTRODUCTION vasega vers sess etre scans keseuss casa E E E 4 OTE SETUP ara cece cece ue cw E E E 4 General Comme S ss asissoinseisninreiooisesseenini siasa n na eiar a E aeae Eare Eo nein edian EEEE e 4 QE 1HC assembly with Series 40 chambers seeessessssseeecoccssssceccccoossscecocoossssssscceccosssssececossssssececssssss 5 QE 1HC assembly with glass bottomed culture dishes ssssssssccccsssssssscssccccscccssssssscccossseeees 5 QE 1HC SYSTEM SETUP including TCM 1 and CL 100 eeeceosssssscececccssssceccccosssssececosssssseeeessssso 6 Goncral commeNfS 55555 chasse deasvseseesssonigeteewessceiescsssasvsensctsserdoesabeepseelvaseseusvovskusssnedeesencondesivssietvescapaaeeedserss 6 Connecting the TCM 1 Thermal Cooling Module heat exchanger ssssssssscccssssssssssssccooees 6 Loading the chamber OF dish sescsis cecsecesvesccssecesenscdeuters tecesstvscsetsessesetwucseuvsecesessenssecwarseseeutevesecwasdeatecestevss 7 When usme THE GIST COVER sssini EE EES 7 Condensation issues when using the COVED o oooeesssnnnsssseenssssserssssseerss
3. bubbles are gone apparatus is filled Add water to the reservoir until it remains full and then seal with the screw 8 Allow the pump to run and check for leaks Loading the chamber or dish 9 Skip step 9 if using a Warner Series 40 chamber Select an appropriate adapter ring for your Petri dish Place the ring notch side down into a flat surface and gently press the dish into the ring The dish is now ready to mount into the QE 1HC platform 10 Place the chamber or dish into the QE 1HC The image to the right shows a Willco Wells dish properly placed within the QE 1HC When using the dish cover 11 If needed attach a clean cover glass onto the dish cover This is done by removing the rubber o ring and old cover glass from the dish cover Replace the cover glass and secure it into place using the o ring A replacement o ring is supplied with the QE 1HC 12 Mount the dish cover over the Petri dish and onto the QE 1HC The dish cover press fits into place Condensation issues when using the cover Condensation can occur on the coverglass window of the dish cover If needed an optional air line may be attached to the cover to mitigate this artifact 1 Attach one end of the supplied 1 16 ID tubing to your air source and the other to the white input port on the dish cover 2 Place the dish cover into place on the stage insert and turn on the air flow Set the flow rate to the minimum value needed to keep the viewing wind
4. etergents We do not recommend the use of solvents such as ethyl alcohol EtOH as this will likely dissolve the glue holding the stainless steel plates onto the base For similar reasons do not autoclave Warranty and service Warranty The QE 1HC is covered by our Warranty to be free from defects in materials and workmanship for a period of one year from the date of shipment If a failure occurs within this period we will either repair or replace the faulty component s This warranty does not cover failure or damage caused by physical abuse In the event that repairs are necessary shipping charges to the factory are the customer s responsibility Return charges will be paid by Warner Instruments Service We recommend that all questions regarding service be referred to our Technical Support Department Normal business hours are 8 30 AM to 5 00 PM EST Monday through Friday Our offices are located at 1125 Dixwell Avenue Hamden CT 06514 We can be reached by phone at 800 599 4203 or 203 776 0664 Our fax number is 203 776 1278 E mail us at support warneronline com or through the web at http www warneronline com contact html Specifications Minimum Temperature 0 C Maximum Temperature 50 C Accuracy 0 1 C Water Jacket Ports Delrin barbed connectors 3 175 mm OD Conroier CL 100 or CL 200 Bipolar Temperature Controller Physical Dimensions 64 0 x 92 2 x 10 5 mm Dx Lx H Cable Length 2 4 m Connector Type 15 pi
5. ew the assembly instructions for the TCM 1 We will use a modified procedure The goal is to connect flow lines from the fill reservoir of the TCM 1 to the QE 1HC and back to the fill reservoir 1 Begin by connecting the two WATER JACKET CONNECTING TUBES supplied with the TCM 1 to the upper and lower flow ports on the rear of the TCM 1 as shown to the upper right Now cut the Tygon tubing supplied with the TCM 1 into two equal length pieces This tubing will run from the QE 1HC to the TCM 1 Make the length as short as is needed Attach one end of the two Tygon tubes to the QE 1HC perfusion flow ports as shown to the lower right Join the open ends of the Tygon tubes from step 3 to the two WATER JACKET CONNECTING TUBES from step 1 on the rear of the TCM 1 You should now have a complete and closed tubing loop from the TCM 1 to the QE 1HC and back Next remove the screw from the top of the TCM 1 to Vi 1 upper flow pO reveal the internal fluid reservoir fill port Using the supplied fill bottle add the algaecide included with the TCM 1 to the fill reservoir Refill the fill bottle with clean water and add to the reservoir Repeat until the reservoir is full I Warner Instruments QE 1HC Rev 090218 7 Leave the fill port open and power the TCM 1 Allow the pump to prime and wait for the circulating water to fill the QE 1HC You can check filling status by watching for bubbles in the tubes Once all
6. ltier electronics Peltier driven heating and cooling is a proven and direct method for providing temperature regulation to devices comprised of metal components The quiet performance of the Peltier device coupled with the high thermal conductivity of the metal components makes for a robust and efficient system The efficiency of specimen heating and cooling is affected by the presence of condensation Good thermal transfer efficiency also depends on a high quality mechanical attachment of the dish to its support For this reason we provide several aluminum adapter rings to bring the chosen 35 mm culture dish into close apposition with the stage insert The base of the QE 1HC also has a thermal barrier designed to reduce the occurrence of condensation when the unit is cooled to below the dew point Features of the QE 1HC include v Open design allows easy access Temperature control from 5 C to 50 C Designed for 40 Series chambers and 35 mm culture dishes Quick exchange of chamber or 35 mm culture dish Removable perfusion and suction tube holders Adapters for dishes by Willco Wells IBIDI WPI Corning MatTek and Falcon Stainless steel plates for attachment of magnetic holders and dish clamps KK N NN NN Fits all Series 20 stage adapters 3 n I Warner Instruments QE 1HC Rev 090218 INTRODUCTION The QE 1HC Quick Exchange Heated Cooled Platform is a versatile base for Warner s Series 40 chambers as well as many popular 35
7. lture dishes The QE 1HC provides an ideal environment for rapid screening of cell cultures grown in 35 mm culture dishes and culture dishes from several manufacturers are supported by the platform Generally speaking a culture dish adapter ring must be used to provide a secure tight fit between the culture dish and the platform 1 Begin by removing any installed chambers from the QE 1HC base Also remove any retaining clips and perfusion lines Do not however remove the TCM 1 heat exchanger lines 2 Fit your culture dish with an adapter ring Use the table below as a guide in selecting the correct ring 3 Place your culture dish with adapter ring into the QE 1HC base 5 I Warner Instruments QE 1HC Rev 090218 QE 1HC SYSTEM SETUP including TCM 1 and CL 100 General comments and TCM 1 is shown to the right Instructions for use of the CL 100 and TCM 1 are left to their respective user s manuals attach the heat exchanger to the QE 1HC and to check for a secure leak free connection This is followed by placing the QE 1HC into an appropriate A fully assembled QE 1HC including the CL 100 The general system assembly procedure is to stage adapter A connection is then made between the QE 1HC and the temperature controller Finally the dish or chamber is inserted into the platform perfusion lines positioned and the experiment begun Connecting the TCM 1 Thermal Cooling Module heat exchanger First revi
8. n Male D Weight 105g Warranty 1 year 10 n I Warner Instruments QE 1HC Rev 090218 Cat No 64 1659 64 0352 64 1633 Parts and Accessories Model QE 1HC CL 100 TCM 1 Product Quick Exchange stage incubator Bipolar Temperature Controller Thermal Cooling Module Accessories Replacement parts 64 0297 64 0296 64 0291 64 0295 64 0107 64 1404 64 1553 64 1403 64 1554 SA 20KZ SA 20LZ SA NIK SA OLY 2 TA 29 PT QE1 MAG 6 ST QE1 MAG 7 Stage Adapter for the K stage Stage Adapter for the Zeiss Stage Adapter for the Nikon 108 Dia Stage Adapter for the Olympus 110 Dia Cable with Bead Thermistor Perfusion Tube Mini Magnetic Clamp for 18 gauge tube Suction Tube Mini Magnetic Clamp for 16 gauge tube 11 I Warner Instruments
9. ow clear 3 Excess air will escape from the QE 1HC from underneath the installed Petri dish A completed assembly with water and air flow lines attached is shown to the right 7 N I Warner Instruments QE 1HC Rev 090218 When not using the dish cover 11 When using RC 40 Series chambers or 35 mm culture dishes without the benefit of a dish cover then care should be taken to secure the dish into the platform This is achieved by use of the supplied magnetic hold downs 12 An example of these hold downs in use is shown to the right Continuing with general assembly 13 Using the supplied magnetic holder place the TA 29 thermistor into the bath to monitor the bath temperature The TA 29 can be connected directly to your Warner temperature controller or to the input of a voltmeter 14 Attach the control line of the QE 1HC base to the associated 15 pin D connector on your temperature controller CL 100 or CL 200 socket labeled HEATER COOLER 15 Place the QE 1HC into an appropriate stage adapter and place the assembled olatform adapter complex onto your microscope stage 16 Insert the perfusion tubes into the magnetic holders and attach the perfusion PE flow lines to the perfusion tubes The larger diameter tube is the aspirator 17 Place the perfusion tubes into position on either side of your chamber 18 Place the QE 1HC into an appropriate stage adapter and then place the assembly onto your micro
10. scope stage 19 The completed assembly is ready for use OPERATION 1 Turn onthe power to the TCM 1 2 Turn on the power to the CL 100 or CL 200 hereafter referred to as the temperature controller 3 Set the temperature controller MODE SELECTOR SWITCH into auto the INTERNAL EXTERNAL SELECTOR SWITCH to internal see the temperature controller User s Manual and the LOOP SPEED SELECTOR SWITCH to medium 8 n I Warner Instruments QE 1HC Rev 090218 4 If desired place the TA 29 thermistor into the bath to measure the temperature at a point of interest 5 Adjust the SET TEMP on the temperature controller to the desired operating temperature Allow the system to thermally stabilize Monitor the temperature at the point of interest using the TA 29 thermistor 6 Make any temperature adjustments on the temperature controller to compensate for heat loss to the surrounding area Additional adjustment of the heater set point may be required if any of the following change substantially during an experiment Efforts to minimize these factors will be rewarded e Perfusion solution flow rate if present e Temperature of solution entering the chamber if flowing solutions e Ambient room temperature e Air currents around chamber 9 n I Warner Instruments QE 1HC Rev 090218 APPENDIX Cleaning Maintenance The QE 1HC is constructed of anodized aluminum and stainless steel It can be easily cleaned with common laboratory d
11. ssserossssreressseerssssseresssseerssssseeeo 7 When not osina the dish COVCTssisssrsosisorsnisseccsnsonoros ipus bus natione urA ENEAN ONE EEEE OARENIEN Ei 8 Continume with gencral assem ly sss ssaccacciesssevessaceocssescatevcstounesssasevatesteensacsssusnvostensessssansessoneensssssesexcoees 8 OFERA TION sara es pes ea aca E Gansu uhssaashusvtasyuehsucusiues EE EE Grebe Gerectsscusstbcsads 8 PPP ONO YA oae E E E 10 C leanino Mante aC sossen niinen ENNE ASENNE RNN Aana aN E EEN ONN NENNEN 10 Warranty and SCT WACO oars os ccecscnaatetyccsccucecanstscscoseubsceunstadesscecesoususssseeoscensacvseietecacsuss euE r 10 WOR O Y eer E A E E TE E E T E 10 E EENAA EEE E A O E E P E esc ears A AE sees 10 Specilicafioh Sessien aa aa anea Eaa A ANAA ANRE SASE ENOR ALENEN ara e iaa 10 Parts and ACCESSOrICS isc coc ceaceesecvesecsnausssesvececcecescecsssuepensndsaseessecaccosss apecseeessausssvescennecesessesvascessseageunrs gt 11 f J Warner Instruments QE 1HC Rev 090218 DESCRIPTION The QE 1HC 35 mm Quick Exchange Heated Cooled Platform from Warner Instruments is designed to conveniently provide rapid heating and cooling of Series 40 chambers and 35 mm culture dishes This versatile system readily accommodates culture dishes from several manufacturers and will adapt to any microscope stage supported by Warner Instruments An integral water jacket uses recirculating water from Warner s TCM 1 Thermal Cooling Module to remove excess heat from the QE 1HC s Pe
12. ture controller The dual channel CL 200 is particularly useful for this configuration since one channel can control the QE 1HC while the other controls the SC 20 QE 1HC SETUP General Comments In addition to the Series 40 chambers the QE 1HC is designed to accept any of the popular glass bottomed 35 mm culture dishes manufactured by Willco Wells IBID WPI Corning MatTek and Falcon chambers and dishes sold separately Chambers and culture dishes are secured into place by the included magnetic retaining clips AR 4 adapter rings may be needed to ensure a close fit between the culture dish and platform base see page 5 In all cases however solution flow is provided by the perfusion and suction tubes The QE 1HC is supplied with removable perfusion tubes and magnetic retaining clamps A short length of silicone tubing is included to facilitate the attachment to PE flow lines QE 1HC Rev 090218 QE 1HC assembly with Series 40 chambers Series 40 chambers are ideal inserts for use with the QE 1HC and the platform has been optimized for this application Warner Series 40 chambers are available in several designs to accommodate different applications a representative sampling is shown to the right 1 Begin by removing any retaining clips perfusion tubes and installed chambers from the QE 1HC base 2 Mount a coverslip into your Series 40 chamber and place into the QE 1HC base QE 1HC assembly with glass bottomed cu
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