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Evolution 500 User Manual

1. Evolution 500 User Manual Note This manual is being revised so some of the information you will find in it is out of date Please accept our apologies for any confusion this may cause Any reference to Unicam Spectronic Instruments or Thermo Spectronic has changed to Thermo Electron Corporation and the contact and trademark information has also changed On the next page you will find a current disclaimer and up to date contact and trademark information Please contact Thermo Electron if you have any questions or concerns P N 10 500111 Thermo ELECTRON CORPORATION The information in this publication is provided for reference only All information contained in this publication is believed to be correct and complete Thermo Electron Corporation shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material All product specifications as well as the information contained in this publication are subject to change without notice This publication may contain or reference information and products protected by copyrights or patents and does not convey any license under our patent rights nor the rights of others We do not assume any liability arising out of any infringements of patents or other rights of third parties We make no warranty of any kind with regard to this material including but not limited to the implied
2. and will take at least 15 minutes This option applies where there are significant wavelength errors or where the original calibration has been lost If a Mercury lamp calibration is required other than because of the replacement of a major optical 50 Issue 1 August 2003 9 7 9 8 9 9 Nicolet Evolution 500 User Manual component then it is likely that there is a serious problem with the spectrophotometer that requires investigation by a trained engineer Absorbance Accuracy If for any reason the absorbance accuracy is suspect first check the instrument using a set of traceable certified calibrated absorbance standards see Section 9 11 If incorrect results are still returned a calibration of the detector gains is possibly required this procedure can be carried out by a user via a PC in terminal mode For further information contact your local customer support office Cleaning the Optics Evolution 500 instruments have tri sealed and coated optics which means that very little maintenance is required and mirrors should not require cleaning However dependent on the local environment and the usage rate of the Deuterium lamp the reflectivity of M1 the lamp mirror will degrade with time this effect can be seen by eye as a milky coating on the mirror surface No other mirror should be cleaned Cleaning the M1 mirror requires that the Deuterium lamp is switched on to adjust the mirror when clean For this reason only those pers
3. be followed by the user to ensure safe operation and retain the apparatus in a safe condition The apparatus has INSTALLATION CATEGORY II and has been designed for indoor use Electromagnetic Interference This equipment has been tested and complies with the following EN50082 1 Generic immunity standard including IEC801 2 IEC801 3 IEC801 4 IEC801 5 IEC801 6 EN50093 Electrostatic discharge Radiated electromagnetic fields Electrical fast transients Electrical surges Induced RF Mains variation Note Shielded Cables All accessories computer and peripherals should be connected to the Evolution 500 system using shielded cables to maintain the appropriate emission compliance 38 Issue 1 August 2003 9 1 9 2 Nicolet Evolution 500 User Manual SECTION 9 GETTING THE BEST FROM YOUR NICOLET EVOLUTION 500 INSTRUMENT Introduction The Nicolet Evolution 500 double beam scanning UV Visible spectrophotometer offers one of the best value for money systems on the market This section has been included to assist you in maintaining this performance during the working life of the spectrophotometer System Overview The Nicolet Evolution 500 is a double beam spectrophotometer built around a robust aluminium base casting The optics are mounted above the casting in a sealed enclosure The control electronics comprising a processor board and a power supply board are mounted below the casting The design makes provision
4. See Fig 10 5 2 Remove the eight retaining screws and remove bottom plate See Fig 10 5 3 Fuse locations are shown in Fig 10 6 Remove fuses by holding the top cap pressing down and turning anti clockwise This releases the catch allowing the cap and fuse to be extracted 4 Replace the faulty fuse then replace the bottom plate by reversing the procedures in 3 and 2 61 Issue 1 August 2003 Nicolet Evolution 500 User Manual Fig 10 4 Mains Fuse replacement Soup Wl ooer DRADAPIILUL YUGLLECL LLL Retaining screws Bottom plate Fig 10 5 Bottom Cover removal 62 Issue 1 August 2003 Fig 10 6 Internal fuse locations Fuse FS1 FS51 FS101 FS151 FS152 FS201 FS202 FS203 FS204 Value 2 0A 6 3A 6 3A 1 0A 1 0A 50 mA 1 0A 1 0A 2 0A Nicolet Evolution 500 User Manual Part Number 2422 086 01031 2422 086 01136 2422 086 01136 2422 086 01021 2422 086 01021 2422 086 01002 2422 086 01021 2422 086 01021 2422 086 01031 63 Issue 1 August 2003
5. The present calibration cannot be supported by the hardware Please rerun a Mercury Wavelength Calibration See section 9 6 1 of the Local Control Software Manual and call Customer Support if the problem persists E3068 SAMPLE BEAM GAIN PROBLEM The sample channel gain cannot be set low enough to avoid saturating the detector In general this error arises if the measurement is not being carried out under the same conditions as the EHT calibration This can be caused by a light leak It can also arise after a lamp replacement Although it is not normally necessary to recalibrate the EHT tables this will need to be done if the new lamp is significantly brighter than the old one Please check that the lid is properly closed If this fails try recalibrating the EHT See Section 9 3 of the Local Control Software Manual E3069 REFERENCE BEAM GAIN PROBLEM This error is analogous to E3068 above E3070 DARK SIGNAL PROBLEM The dark signal is too large This may be due to a light leak but can also occur on PMT instruments when the EHT has not been optimised for the current operating conditions Please check that the lid is properly closed If this fails try recalibrating the EHT See Section 9 3 of the Local Control Software Manual E3071 DARK SIGNAL PROBLEM The dark signal cannot be correctly nulled Usually caused by a light leak Please check that the lid is properly closed If this fails try recalibrating the EHT See Section 9 3 of the Local Co
6. on the Lamps Function Key on the HOME Page then on the Energy Function Key on the LAMPS page In VISION click on the lamp symbol in the VISION status box then click on Measure Energy New lamp fitted Highly absorbing accessory fitted use High Abs profile Instrument used in very cold or very hot laboratory Lamp selection mirror cleaned Filters or grating changed 9 5 3 Optical Initialisation Every time the instrument is switched on the following sequence occurs Electronics All power supplies are tested All memory is tested The detector pre amplifier offset voltage is adjusted Filters and Slits The movement of the filter wheel is tested and it is placed at the correct filter The self test if fitted is driven to the clear beam position The variable slit plate if fitted is driven to the widest slits 47 Issue 1 August 2003 Nicolet Evolution 500 User Manual Tungsten lamp The lamp is switched on and its current checked The monochromator is driven to its zero order sensor The monochromator is then driven to approximately 500 nm The lamp select mirror is scanned to find the exact position of the tungsten lamp Monochromator Using the tungsten lamp the monochromator is scanned to find the exact position of zero order light Slits Readings are taken using each slit in turn i e at each bandwidth and the EHT photomultiplier supply optimised to give the factor for each slit These factor
7. Printer Serial Parameters Epson and HP Paintjet HP Laserjet Baud rate 9600 l P O P PORT Serial Parity None BAUD RATE 9600 Data 8 bits ROBUST XON Off Stop bit 1 bit DTR POLARITY High 17 Issue 1 August 2003 Nicolet Evolution 500 User Manual 5 1 2 Using your own Printer If you are supplying you own printer you need to acquire a suitable cable to connect it to the instrument The cable should be a null modem serial interface cable with a 9 way D type female connector for connection to the instrument The connections to the 9 or 25 way printer connector should be as listed in Table 5 2 Table 5 2 Serial Printer Platter cable 4013 172 73540 Instrument Printer Plotter 9 Way pin 25 Way pin 1 4 oF a co r ow com M No Connection SCTEEN Ssureen 18 Issue 1 August 2003 5 2 Nicolet Evolution 500 User Manual Connecting up a Computer based Nicolet Evolution 500 system On a computer based Evolution 500 system the printer supplied must be connected to parallel interface connector 1 LPT1 whilst a plotter must be connected to serial interface connector COM2 1 Using the cable supplied with the PC software connect the Evolution 500 spectrophotometer to the computer as shown on Fig 5 2 2 Check all PC interconnections previously connected Section 4 3 2 Ensure the connectors are firmly secured to their respective mating connectors by tightening the jackscrews evenly on each side 3 If
8. compared to a nominal expected value If the unit is supplied fitted to the spectrophotometer these values are the values recorded from the spectrophotometer when first fitted in the factory If you require traceable certified values see the Calibration Validation Unit CVU detailed below 9 10 2 Literature To complement the Evolution 500 instrument a set of Application Notes has been produced which covers the fundamental principles of instrument calibration AN 01142 Wavelength Accuracy Its Measurement and Effect on Performance in UV Visible Spectrophotometry AN 01143 Absorbance Accuracy Its Measurement and Effect on Performance in UV Visible Spectrophotometry AN 01144 Stray Light Its Measurement and Effect on Performance in UV Visible Spectrophotometry 52 Issue 1 August 2003 9 11 Nicolet Evolution 500 User Manual AN 01145 Changing scan speed and the associated effects on spectra in UV VisibleSpectrophotometry AN 01146 Instrument Resolution Its Measurement and Effect on Performance in UV VisibleSpectrophotometry Contact your local Thermo Electron Customer Support organisation if you wish to obtain these notes Third Party Validation All Quality or Accreditation schemes require irrefutable proof of instrument calibration and this is achieved by reference to a third party This can be achieved either by receipt of a Certificate of calibration from this independent source or by the use of calibration stand
9. is also possible to measure the lamp energies as a percentage of the energy measured when the lamp hours were last reset The status box also flags the recommended lamp replacement time by colouring the hours background red when the recommended number of hours has been exceeded Allow the lamps to warm up Although the instrument can be used after approximately three minutes better stability will be achieved after 10 minutes for the Tungsten lamp and 30 minutes for the Deuterium lamp Always run a lifetime initialisation after replacing a Tungsten or Deuterium lamp This resets the position over which the moving mirror searches for peak energy throughput 41 Issue 1 August 2003 9 4 Nicolet Evolution 500 User Manual which will vary from lamp to lamp This is done in Local Control software by selecting the OPTICS option on the SETUP INITIALISE page or from the Commands Initialise Lifetime menu of the Vision software Detectors The Nicolet Evolution 500 spectrophotometer has a photomultiplier detector 9 4 14 Photomultiplier Detectors Photons of light strike the coated surface of the photocathode which emits a number of electrons for each photon These electrons are directed by an electric field to a dynode which emits a number of secondary electrons for each electron which strikes it The process is repeated through a total of nine dynodes with the number of electrons emitted increasing each time Finally the electrons hit the ano
10. required connect the printer supplied to the parallel interface connector LPT1 on the rear of the computer base unit using the cable supplied with the printer At one end of the cable is a 25 way D type plug Fit this into the PARALLEL 1 LPT1 socket of the computer drawing the plug carefully into the socket by tightening each jackscrew alternately a turn at a time Connect the 36 way plug at the other end of the cable to the mating socket on the printer Secure in position using the clips on the printer 4 If required connect the plotter to the serial interface connector COM2 on the rear of the computer base unit using the cable supplied with the plotter Ensure the connectors are firmly secured to their appropriate mating connectors at both ends of the cable 5 2 1 Setting up the Printer Plotter Various printers plotters may be used each being supplied with its own manual which should be consulted for full instructions on setting up and operation When using Vision software on the PC any parallel printer supported by Windows can be used to obtain hard copy output 19 Issue 1 August 2003 Nicolet Evolution 500 User Manual t JA ADL LUI esnoy a Ja nduoy SUEN Qw 25w cable 4013 172 73540 J81Ll 1d suleW asno 05565 ZAT ETOP e qeo 1ejuiid uidgg mgz pJeoqAey a JoyuoN ZI c WOO suey sujew Jo3iuoy pJeoqAey Tld O8plA T WOJ QTTe8 ZLT ETOF e qeo mp B Jeddis jedns cECSH sule
11. surface must be level and the spectrophotometer must not be placed on any type of cushioning as this could block ventilation When siting the system consider the environment in which the equipment is to be used Some of the factors that may adversely affect the operation of the spectrophotometer and associated equipment are given below 4 1 1 Static electricity Static electricity can permanently damage electronic components which do not have to be exposed directly to a static discharge for damage to occur For instance an external static charge may be conducted through the pins of an unused exposed connector to damage internal components Avoid non conducting carpets in the area surrounding the equipment Anti static mats if large enough can be placed over conventional carpeting Man made fibres and some plastics used in chairs etc can build up large potentials 4 1 2 Temperature The stability of the spectrophotometer may be affected by marked changes in temperature For optimum operation the temperature should be maintained in the range 5 to 40 C and vary by less than 2 C 4 1 3 Direct Sunlight Avoid large window areas Direct sunlight will cause heating of the spectrophotometer and will contribute to stray light 4 1 4 Vibration Floor vibration caused by lifts air conditioning of refrigeration units etc or loud acoustical noise may affect the performance of the instrument Minimise this situation wherever possible 4 1 5 D
12. system interconnections Figs 5 1 and 5 2 provide diagrammatic representations of the interconnections required for the installation of each version of the Nicolet Evolution 500 5 1 Connecting up a Standalone Nicolet Evolution 500 based system On the Nicolet Evolution 500 Standalone only one device can be connected The device must therefore be fitted with a serial interface adapter Connect the required printer or plotter to the 9 way D type connector at the side of the Spectrophotometer Ensure that the connectors are firmly secured to their appropriate mating connectors at both ends of the cable No cables are supplied with the Nicolet Evolution 500 instrument unless the printer is supplied by Thermo Electron If you supply your own serial printer you must supply your own printer cable gw 25w serial cable 4013 172 73540 M 5 Mains Mains RS232 Super Sipper Mains Plotter Fig 5 1 Connections to a Standalone Nicolet Evolution 500 system 16 Issue 1 August 2003 Nicolet Evolution 500 User Manual 5 1 4 Setting up the Printer Plotter This Section gives specific details concerning the setting up of printers plotters for use with the Local Control software on a Nicolet Evolution 500 UV Visible Spectrophotometer The printers supported are as follows Epson 9 pin dot matrix printer generic driver Hewlett Packard HP Deskjet printer generic driver Hewlett Packard HP Laserjet printer generic driver Hew
13. the Mains sse 11 4 3 Locating the Computer if appropriate esee 13 4 4 Connecting the computer to the Mains sess 14 4 5 Locating the Printer Plotter if appropriate uurusrsnnnnneennnnnennnnnnnnnnnnnnnnennnnnnn namen 14 4 6 Connecting the Printer Plotter to the Mains sse 15 SECTION 5 SYSTEM INTERCONNECTIONS 242402240444400nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 16 5 1 Connecting up a Standalone Nicolet Evolution 500 based system 16 5 2 Connecting up a Computer based Nicolet Evolution 500 system sssuss 19 5 3 System Interconnection Summary ersssessssnnssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nen 21 SECTION 6 SYSTEM POWER UBP uunsnssesnsssennsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsnnnnn nassen inneren 22 6 1 Powering up a Standalone based Nicolet Evolution 500 system ss 22 6 2 Powering up a PC Controlled system using VISION software sess 24 SECTION 7 TROUBLESHOOTING 222402240444400nsnnnnnnnnnnnnnennnnnnnnnnnnnnnnnnnnennnnnnnnnnnnnnnnnnnnnn nn 25 Tel FAUCET Heeres UE POR a Ce PER x cR E 25 SECTION 8 SYSTEM DESCRIPTION 0us440srsnsunnnnonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn en nenas 31 9 1 System COMPOSIIO Ms uae tine Et ea FER R
14. to ON at power up and then each one is turned off as the system it reports is found to be OK Fig 7 1 Evolution 500 input and output connections and status LEDs 26 Issue 1 August 2003 Nicolet Evolution 500 User Manual 7 2 Evolution 500 Error Numbers and Messages When the instrument is switched on the software initializes the instrument and then continues to monitor its operation when in use If a problem occurs a software message will be displayed with an error code Warning messages indicate that data quality may be impaired In the event of a fatal error data collection ceases user intervention is required Self help by the user is possible in response to some error messages and these are listed below If the measures suggested fail or the error code is not included here please contact Thermo Electron Customer Support quoting the relevant error message number 7 2 1 Warnings These warnings may be generated when the instrument is under computer control W1017 The requested command to the spectrophotometer cannot be run as it is busy running another command W1026 SELF TEST NOT PRESENT An attempt has been made to move self test wheel when the accessory is not fitted W1037 CLOCK FAULT SEE USER MANUAL The battery in the real time clock chip has failed This should be replaced by a qualified engineer 7 2 4 Fatal Errors E3001 CELL PROGRAMMER NOT PRESENT The cell hardware is not connected E3002 CELL PROGRAMMER
15. to the local Service Engineer One of the major design criteria of the Nicolet Evolution 500 instruments was to make User Servicing available where possible so access to the Default Baseline is one of these many Servicing tools The Default Baseline is run as follows in the factory on clear beams Evolution 500 Series 190 to 900nm 1 5nm bandwidth 0 1 nm data interval It is non volatile and is stored within the instrument on power down As can be seen from the above the entire wavelength range is scanned at the smallest data interval with a 0 5 sec integration time per point therefore the process will take at least 1 hour The Default baseline is run from the SETUP INITIALISE page of the Local Control Software or the Commands Default baseline of the Vision Software 9 5 1 2 User Baseline As the name suggests this is a baseline set up which can be established for any given set of parameters scan range bandwidth etc to compensate for the application environment currently in use over the wavelength range required and is performed under exactly the same condition as the current scan method This is volatile and is lost when power is removed from the instrument 43 Issue 1 August 2003 Nicolet Evolution 500 User Manual 9 5 1 3 Zero This is the beam balance at a FIXED wavelength and is again used to remove any Offset for a given set of parameters wavelength bandwidth etc to compensate for the application enviro
16. with an integration time of 15 milliseconds By slowing the scan speed and or increasing the data interval the integration time can be increased and consequently noise decreased 9 5 4 2 Intelliscan Intelliscan is a means of optimising the noise on a spectrum by varying the integration time at each data point and therefore the scan speed over the spectrum How Intelliscan Works As the energy hits the detector an analogue signal is produced which is converted to a digital signal by the analogue to digital converter ADC For the ADC to work effectively the signal reaching it has to be within a preset range If the signal is outside this range then it is amplified The level of amplification is called the gain So if a given signal is below the range specified for the ADC another detector reading is taken and the gain increased This process continues with the gain increasing until the signal level is within the acceptable range A measurement is then taken and the spectrophotometer moves on to the next wavelength The level of gain has no effect on the strength of the signal it simply multiplies up a weak signal to fit the appropriate ADC range The level of gain is however a direct indicator of the amount of energy hitting the detector which in turn is dependent on the combination of source energy less absorbance of the sample Therefore the level of gain set at any wavelength can be used to set the integration time required at that wavele
17. 0 User Manual dark as much as possible and install it in dim light It can take 24 hours to recover from direct exposure to fluorescent lighting Prolonged use at very high EHT levels can also cause noise degradation which may take days to recover If you use your Evolution 500 continuously at 0 2nm bandwidth or continuously with highly absorbing sample and reference accessories then its noise performance under high energy conditions will be worse for some time afterwards A photomultiplier detector is capable of very high sensitivity or very low noise but not both at the same time Key Concepts 9 5 1 Baselines and Zero 9 5 1 1 Default Baseline On any double beam system there will always be a process of balancing the two beams striking the detector Clearly this process will be affected by any major difference between the sample or reference beams by definition this is how a measurement is made Therefore a reference level i e the energy reaching the detector with both beams clear must be established for the wavelength range of the instrument This is known as the Default Baseline and even with both beams clear this value will be changed very slightly by slow long term effects e g changing optical characteristics Historically this Default Baseline was stored deep within the electronics of the spectrophotometer and if any offset did occur in the baseline because of these long term effects then these were remedied by a call
18. 91 53 5281 146 3273 391 934 1042 2919 W L Cal 1 001403E 00 6 402363E 07 3 312368E 11 0 0E 00 VAR PD UV2 200 012345 190 900 512 nol 2 0 NOWAV NOCOR 54 Issue 1 August 2003 Nicolet Evolution 500 User Manual 9 13 User Access Your instrument was designed for ease of maintenance and as such a number of the modules present in the instrument can be replaced by the user Some of the operations do however require specialist knowledge or formal safety training therefore three categories of component exist These are described below Category A Consumables These items are replaceable by any non technical user who has read Section 3 Safety of the UV Series Installation and Maintenance Manual Tungsten Lamp Deuterium Lamp All Accessories Mains fuse Category B Non critical components These are items which require no special alignment jigs or tools They can be replaced by a technically qualified person who has attended an approved Thermo Electron training course Disc drive Lamp mirror M1 replacement and cleaning Printed circuit boards Lamp mirror drive assembly Grating holder assembly Filter assembly LCD Assembly Wavelength drive assembly Modulator assembly Category C Critical components These are items or modules that require specialised alignment tools and are only replaceable by a Thermo Electron service engineer or appointed agent Collimator mirror Post sample compartment optical system Detector asse
19. EX e REIR ERE o age enge 31 8 2 Technical Specifications 240444400nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nen 36 8 3 Regulatory Notices 22200umn4nn E EE EEE IA EE SEESE EER 37 SECTION 9 GETTING THE BEST FROM YOUR NICOLET EVOLUTION 500 INSTRUMENT 39 9l MTROGUCTION ee oe ee eee ets Ee t een E aO Hg EE ee REPE OEc dn 39 9 2 System Overview nnnnnsnnsnsnnnnnnnnnnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnnnnannnnnnnnnnnnnnnnnnann 39 9 3 LAMPS ED E 41 9 4 Detectors an a Roe epe eene Fa Due dere ue ciet ee a Due c te Due Oe ebay 42 9 5 Key Concepts ine caeca een d aec ea dL Tap vea e d c RL eae ex da vadis 43 9 6 Wavelength Accuracy and Recalibration eeeneeene ene 50 9 7 Absorbance Accuracy nnesersnneenesnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsn nasse nnnn nenn nnnn nnns 51 9 8 Cleaning the Optics ilico gets tee ade aida neun 51 9 9 Standby and Wakeup i siisii rires onanii ioii ana iani nennen nsn ns sn rte enne iaaiiai 51 9 10 Validation gt Data ineat Tuae ee end dac v dee e ann 52 9 11 Third Party Validation nn er en em 53 9 12 Instrument Parameters arn oiiaii eniad a at eoa ea AE droa nenn 54 9 19 User ACCOSS nn DA do ceil Glee epe a Fe 55 9 14 Evolution 500 Recommended Service Intervals sss 56 SEGTION 10 MAINTENANGE Er 2442 Heilen Bine pa race P UR EM e dep keen o 57 10 1 ROUtIN
20. G MaINTGNANCC 57 10 2 Cleaning Instrument Exterior sssssssssesessseeesee esent nnns 57 10 3 Removal and Replacement of Tungsten Halogen Lamp sss 57 10 4 Removal and Replacement of Deuterium Lamp sees 60 105 Renewal of EUSOS 1 2 n ech redes te emp Bede P E Aa HEUS eere nei 60 2 Issue 1 August 2003 1 1 Nicolet Evolution 500 User Manual SECTION 1 OVERVIEW Introduction 1 1 1 Installation and Maintenance This User Manual has been designed to assist you with the installation of a Nicolet Evolution 500 UV Visible Spectrophotometer system Detailed below is how we anticipate this process will occur START HERE This chart assumes that you have not purchased a Validator package and are using this manual to install the system without following a formal validation procedure SECTION 2 Inspect shipment for completeness lack of damage etc Once satisfied move to the next section SECTION 4 Establish a suitable location for the system with sufficient power outlet connections Ensure that all components are correctly set to the supply voltage SECTION 3 Read this section on Safety and ensure all the requirements detailed here are met SECTION 5 Connect the system together as described in the section SECTION 6 Power up the system and follow the descr
21. IST For more information on these and the other validation accessories see below 53 Issue 1 August 2003 9 12 Nicolet Evolution 500 User Manual 9 11 2 Literature The following application notes have been produced to assist users who wish to learn more about certified traceable referencs materials AN 01147 Thermo Electron Corporation Standards for UV Visible Spectrophotometry AN 01150 Metrological Traceability Questions and Answers Contact your local Thermo Electron Customer Support organisation if you wish to obtain these notes Instrument Parameters Certain basic parameters are stored in the spectrophotometer s memory which are essential to the initialisation sequence of the instrument Examples are the full identity of the instrument gain settings for absorbance accuracy and the coefficients of the wavelength calibration software compensation equation These particular parameters are archived in the factory and are recorded on a label attached to the inside of the bottom cover This information will be used by the service engineer to reprogram the flash EEPROM when some parts are replaced and also in the unlikely event of EEPROM corruption The label contents should always be updated by a person who has attended an Evolution 500 service course or a Thermo Electron service engineer when the gains or wavelength have been recalibrated A typical parameter label contains Serial No 012345 Gains 1 0000 2 7499 7 3069 19 97
22. MOVE Failure moving the cell transport Either the mechanism has reported a failure or the bench has timed out trying to move it Ensure that nothing is jamming the cell programmer If the fault persists call Thermo Electron Customer Support E3008 D5 LAMP PROBLEM Failure attempting to switch on the Deuterium lamp Three attempts have been made to strike the lamp This indicates either that the lamp requires replacing or a power supply failure or a blown fuse See Sections 10 4 10 5 E3010 E3011 E3014 E3015 W LAMP PROBLEM Check the tungsten lamp If it has failed replace it See Section10 3 Try switching the instrument off then on again after 10 seconds If this does not clear the error call Thermo Electron Customer Support E3016 ADC 27 Issue 1 August 2003 Nicolet Evolution 500 User Manual The ADC reading for 10V reference signal is less than expected There is either a problem with the reference voltage itself or the ADC Try switching the instrument off then on again after 10 seconds If this does not clear the error call Customer Support E3022 D FAILED The monochromator is at a wavelength where the D lamp is required but after several tries it has not struck See E3008 above E3026 RS232C FAILED The spectrophotometer has not responded Data has been lost Check cable connections between instrument and computer before calling Customer Support E3027 CHECK BEAMS ARE CLEAR During optical initialization it ha
23. TIALISE MONOCHROMATOR TEST OPTICS OPTIMISE MONOCHROMATOR SET DEFAULTS PLEASE WAIT Note Should the display light up but no text appear after the delay noted above check the LCD contrast control underneath the spectrophotometer Rotate fully to the extremes of the available movement At some point the above display should appear TIF IF YOU DON T SEE ANY DISPLAY ROTATE THE THUMBWHEEL UNDER THE INSTRUMENT UNTIL THE DISPLAY RE APPEARS 22 Issue 1 August 2003 Nicolet Evolution 500 User Manual 2 The spectrophotometer should now begin to initialize The following processes are carried out automatically by the instrument during the initialization sequence Each stage is marked with a V pass mark when it has been satisfactorily completed Initialize Optics Filter wheel is driven to end stop Opto sensor is checked Filter is set to clear position Initialize slits Slit plate driven to end stop then back to 1 5nm position Initialize self test unit if fitted Self test wheel driven to end stop back to clear position Wavelength drive is driven back to the end stop sensor Test tungsten lamp The tungsten lamp is turned on and supply voltage checked If tungsten lamp fails to operate deuterium lamp is switched on Initialize Monochromator The monochromator is driven to about 500 nm to ensure light will get through Test optics The lamp change mirror is set up to peak the tungsten lamp energy If the tung
24. W Fig 5 2 Connections to a PC based system Issue 1 August 2003 20 Nicolet Evolution 500 User Manual 5 3 System Interconnection Summary PC Based Evolution 500 system From To Spectrophotometer Computer serial port COM1 Plotter Computer serial port COM2 Printer Computer parallel port LPT1 Monitor signal connector Computer monitor connector Mouse Computer mating connector Keyboard Computer keyboard connector Evolution 500 based system From To Evolution 500 Required printer or plotter serial connector Note 1 To connect any printer or plotter to the Spectrophotometer it must be fitted with a serial interface adapter 2 If connection of both a printer and plotter to a PC based Evolution 500 system is required the mouse type used must be a bus mouse not a serial mouse 21 Issue 1 August 2003 Nicolet Evolution 500 User Manual SECTION 6 SYSTEM POWER UP This section details the application of power to a correctly configured system and the system responses the user should expect in normal operation 6 1 Powering up a Standalone based Nicolet Evolution 500 system 1 Apply power to the spectrophotometer Do not apply power to any connected PC The green power on LED should be illuminated The LCD display will be blank initially and after 1 2 minutes should appear as below 19 01 98 EVOLUTION 500 v7 00 itam SERIAL No 100661 SPECTROMETER INITIALISING INITIALISE OPTICS TEST W LAMP INI
25. Windows 2000 110 130V 9423 UV7 0610E If you supply your own PC to run the VISION software it must have the following minimum specification Pentium processor 64 Mb RAM 100 Mb Hard Disk 1 44 Mb floppy disk drive 2 COMM ports parallel port SVGA graphics adapter and monitor VISIONpro Windows 98 2000 XP or NT4 VISIONsecurity Windows 2000 or NT4 The VISIONsecurity Client software may be run on a Windows 98 PC in conjunction with an NT 2000 network 9W 9W generic null modem UV to PC cable 4013 172 82111 33 Issue 1 August 2003 Nicolet Evolution 500 User Manual 8 1 6 Lamps Evolution 500 W Lamp Evolution 500 D Lamp 8 1 7 Calibration Accessories Calibration Validation Unit NPL Recalibration of CVU NPL Calibration Validation Unit NIST Recalibration of CVU NIST 9423 UV9 0001E 9423 UV9 0002bE 9423 UV6 1250E 9423 UV9 1200E 9423 UV6 1260E 9423 UV9 1260E Validator 5 Log Book 10030101 Dsolve dissolution software version 2 0 Spare IQ documentation 10040871 Dsolve dissolution software version 2 0 OQ documentation 10040881 Dsolve autosampler and dissolution software version 2 0 OQ documentation 10040931 Set of 9 calibrated traceable absorbance filters Recalibration of 9 certified absorbance filters Set of 4 calibrated traceable absorbance filters Recalibration of 4 certified absorbance filters Set of 2 calibrated traceable wavelength filters Recalibration of 2 certified wavelength filters Stray li
26. ards traceable to a recognised National authority e g The National Physical Laboratory or NIST Thermo Electron have produced a variety of aids to assist the user in the process of validation and these are briefly detailed below Further information can be obtained from your local Thermo Electron customer support organisation 9 11 1 Accessories 9423 UV9 0023E MERCURY LAMP The emission lines produced from a discharge lamp are characteristic of the source element The wavelengths are invariable and are therefore fundamental physical standards A low pressure mercury lamp has a number of intense lines that cover a large part of the UV and visible range i e 250 580nm Therefore whilst this lamp can be used for calibration and checking its reliance on fundamental physical parameters also makes it acceptable as a validation tool CALIBRATION VALIDATION UNIT 9423 UV6 1250E CVU NPL 9423 UV6 1260E CVU NIST This unit will provide measurements of Wavelength Absorbance Stray Light Noise and Drift calibrated traceable to The National Physical Laboratory or NIST Supplied with a certificate of calibration this unit requires re calibration at yearly intervals to ensure compliance WAVELENGTH amp ABSORBANCE STANDARDS 9423 179 38901 SET OF 9 ABSORBANCE STANDARDS 9423 185 03001 SET OF 4 ABSORBANCE STANDARDS 9423 185 03111 SET OF 2 WAVELENGTH STANDARDS These standards are provided as calibrated sets traceable to the NPL and N
27. are Evolution 500 Local Control software manual VISIONsecurity Spare PC User manual VISIONsecurity Spare PC Administrator User manual VISIONpro Spare PC User manual VISION ife Spare PC User manual 35 9423 UV5 5000E 9423 UV5 5010E 9423 UV5 5101E 9423 UV7 3500E 9423 UV7 3510E 9423 UV7 3600E 9423 UV7 3610E 9423 UV7 1400E 10 05 0111 10 50 0311 10 04 0311 10 04 0321 10 04 0111 10 04 0211 Issue 1 August 2003 8 2 Technical Specifications SPECIFICATION Wavelength Range Photometric range Detector Bandwidth Best Data Resolution Wavelength Accuracy Wavelength Repeatability Photometric Accuracy Photometric Repeatability Nicolet Evolution 500 User Manual 190 900nm 0 3 to 6A Single photomultiplier 0 2 0 5 1 1 5 2 4 nm 0 1nm t 0 3nm t 0 1nm 0 005 at 2A 0 002 at 1A 0 008 at 3A 0 001 at 1A 0 002 at 2A t 0 005 at 3A EP Photometric Accuracy K2CroO zf 235nm 257nm 313nm 350nm 0 748A 0 010 0 865A 0 010 0 292A 0 010 0 640A 0 010 NOTE It will be necessary to add filter tolerances to these values when checking photometric accuracy 1 Wavelength accuracy using holmium and didymium traceable to the National Physical Laboratory Wavelength repeatability across complete wavelength range using holmium and didymium traceable to the NPL 3 Photometric accuracy at 546nm using neutral density glass filters traceable to the NPL Photometric repeatabili
28. ay use your own computer providing it meets the necessary specification If in doubt contact your Local Sales Office or Agent 4 3 2 Locating the Computer 1 Unpack the computer base unit where the computer is to be used Position the unit to enable access to the rear for fitting connecting cables 2 Unpack the monitor and place on top of the base unit 3 Connect the monitor to the mains outlet on the rear of the base unit using the power lead supplied Note Dependent on the computer supplied the monitor may need to be connected directly to a mains outlet socket 4 Fit the video cable between the video connectors 9 pin D type or 15 pin mini D type connector on the base unit and the monitor 5 Unpack the keyboard and place it in front of and close to the base unit 6 Connect the keyboard to the rear of the computer base unit 7 Unpack the mouse and connect to its mating connector on the rear of the computer base unit 4 3 3 Floppy Disk Drive During transit the computer floppy disk drive may be fitted with a cardboard blank This is to reduce the risk of damage to the drive during shipment Remove the blank before switching on the computer but ensure the blank is retained for future use should it be necessary to move the computer If the cardboard blank is not available fit an unformatted floppy disk or a known faulty disk 13 Issue 1 August 2003 4 4 4 5 Nicolet Evolution 500 User Manual Connec
29. ble to initialise the system with the Deuterium lamp turned on 51 Issue 1 August 2003 9 10 Nicolet Evolution 500 User Manual If the spectrophotometer is in use when the Standby time is reached don t worry the software is intelligent and will not allow the instrument to go to STANDBY until at least 5 minutes have elapsed without any further actions occurring in the software When the instrument has entered Standby mode the red LED on the front of the instrument is illuminated Validation of Data If you have reached this section by reading and implementing all the previous chapters you can now be confident that your Evolution 500 spectrophotometer is at the very least performing to specification but how do you prove it Thermo Electron have produced a variety of aids to assist the user in the process of validation and calibration and these are briefly detailed below 9 10 1 Accessories VALIDATOR PACKAGE Validator 5 Log Book 10 03 0101 Designed to assist with the Qualification requirements of the Regulated Environment this package provides in the form of a log book and guide all the documentation necessary to perform this task Note The content of these two packages is identical the A or E suffix simply defines the ring file format used i e 3 ring American or 4 ring European SELF TEST UNIT 9423 UV6 1200E SELF TEST UNIT This unit will provide measurements of Wavelength Absorbance Stray Light Noise and Drift
30. ck in Line Macros Basic Macros Wro Magna IR Microcast uView Molecular Micro Mapping Momentum MonitIR Mosaic Nexus NicALAS Nicolet Nicolet Compact Four Nicolet Pathfinder Nic Plan NXR NYSTAR OMNI Sampler OMNI Transmission OMNIC Passport Performer Phoenix PLS Quant PLUS ProfilIR Progressive Sampling PROSTAR Prot g QC Complete QCIRCLE Quadrascan QuantPad Quick IR ReactIR Recorder Redundant Aperturing Reflachromat Reflex REGA RESULT RSVP SabIR SAGA Sample Plan Satellite SeaPort Si Carb SID Simul View Slide ON ATR Smart Accessory Smart ARK Smart Collector Smart Purge Snap In Solid Substrate SpecID SpecID Plus Spectra Bench Spectra Fax Spectra Scope SpectroPro SpeculATR SPECTRONIC SPIRIT SST Swap Top SX Sync TACT Talon Targeting Thermo Nicolet Corporation Thunderdome Total Coverage TQ Analyst Triton TruView TVF UpDRIFT Validate Validator ValPro Val Q Val Q DS Vectra Vectra Plus ViewThru Viking Viking II VISION ife VISIONIite VISIONpro VISIONsecurity WinFIRST WinFIRST Lite and WinSpec are trademarks of Thermo Electron Scientific Instruments Corporation a subsidiary of Thermo Electron Corporation Nicolet Evolution 500 User Manual REGULATORY NOTICES ce Notice Marking with the symbol C indicates compliance of this Thermo Electron system to the following European Directives 89 336 EEC Electromagnetic Compatibility directive EMC 73 23 EEC Elec
31. d Replacement of key optical components The instrument will need recalibrating if any of the key optical components are replaced Mechanical wear The instrument has been designed to minimise the effect of wear and this should not be a problem If for any reason the wavelength accuracy is suspect or if a CVU or Self Test has failed on wavelength accuracy first check that the instrument is operating at or about the correct temperature All instruments are calibrated at room temperature and wavelength differences of between 0 1 and 0 3nm per 10 C are not uncommon There are two recalibration options available using either the Deuterium lamp alone or both the Deuterium lamp and optional Mercury lamp The Deuterium lamp recalibration uses the Deuterium emission line at 656 1nm to adjust the software compensation equation and is intended to remove the effects of small movements of optical components and shifts due to temperature shock or vibration If the errors found are linear and no more than 0 5nm then a Deuterium calibration can be used The process will take about five minutes and is fully described in the appropriate software manual This option only applies where an existing calibration is present The Mercury lamp calibration remeasures all 9 relevant emission lines from the Mercury and Deuterium lamps then recalculates and replaces the software compensation equation The process requires the Mercury Lamp accessory part no 9423 UV9 0023E
32. d or Suggested Action Check that the instrument is plugged in and switched on Check the integrity of the mains lead and the fuse in the plug Check that the instrument mains fuse has not blown See Section 10 5 Check that the voltage selector is correctly set The instrument is in standby mode Switch off and then on or activate via RS232 and remote control software LED 1 Power Supply fault Check internal fuses See Section 10 5 LED 2 Boot code fault LED 3 RAM fault LED 4 Error in configuration data LED 5 Flash EPROM fault These faults cannot be dealt with by the user Please note which LED remains on and call Thermo Electron Customer Support Check that both beams are clear The instrument will not initialize correctly if anything is left in either beam at power up Check contrast control lower left side see Section 6 1 Change the tungsten lamp See Section 10 3 Change the deuterium lamp See section 10 4 25 Issue 1 August 2003 Nicolet Evolution 500 User Manual Main fuse FSI Voltage Select pcb Mes m ON OFF er ES switch Fuse pull lever Main fuse Main switch E Ke S Sipper Peltier Power LED Green e Standby LED Red EURER There are five Light Emitting Diodes LEDs visible through a port in the lower left side panel of the instrument They report the status of the hardware and software before the start of the initializing sequence All LEDs are set
33. de where the resulting current leaves the photomultiplier and is amplified in the pre amplifier The sensitivity of the photomultiplier can be varied by adjusting the voltage EHT between each pair of dynodes A full description of EHT is given in Section 9 5 2 Fig 9 3 A photomultiplier detector 9 4 2 Precautions when using a Photomultiplier The photomultiplier detectors used in the Evolution 500 are extremely sensitive devices capable of measuring very low light levels at lower noise than could be achieved with a photodiode detector At normal light levels there is not much noise difference between the two types of detector The two situations where the PMT detector gives a significant improvement are a highly absorbing samples micro cells turbid samples etc and b narrow bandwidths However all photomultipliers have memory effects and their performance can be temporarily harmed by extreme conditions Evolution 500 instruments protect the photomultiplier as much as possible by switching off the EHT supply whenever the sample compartment lid is opened But exposure to high light levels even with no EHT can degrade performance Avoid shining bright lights sunlight or laboratory lighting directly into the sample compartment Do not leave the instrument with its sample compartment lid open If a photomultiplier is replaced keep the new photomultiplier in the 42 Issue 1 August 2003 9 5 Nicolet Evolution 50
34. e cable connecting individual leads as follows Plug pins European N American Code Code LIVE L Brown Black NEUTRAL N Blue White EARTH GROUND E Green Yellow Green A good earth is essential both for satisfactory operation of the instrument and the safety of the user 3 Make sure the Power ON OFF switch is in the OFF position Plug the mains cable into the POWER socket at the rear of the instrument Fig 4 1 4 The mains plug may be inserted into a socket outlet provided with a protective earth contact when all installation work has been done 12 Issue 1 August 2003 4 3 Nicolet Evolution 500 User Manual Locating the Computer if appropriate If there is no requirement to position a computer move on to section 4 5 The computer is designed for use on a normal desk or bench which should be of sturdy construction and of sufficient size to accommodate the computer base unit There should be at least 30mm clearance from any obstruction to allow the circulation of air through the cooling vents Ensure there is ample space at the rear of the base unit for cables The computer should be operated in a room with an atmosphere free from dust and corrosive vapours Note Computer systems commissioned at Thermo Electron in Cambridge will have had the software purchased already installed on the hard disk before shipment A sticker to this effect will be found on the monitor 4 3 4 Using Your Own Computer If required you m
35. e notices is necessary to ensure that the appropriate radio frequency emissions will be maintained within the limits of the specifications referred to in this declaration 1 Issue 1 August 2003 Nicolet Evolution 500 User Manual CONTENTS SECTION OVERVIEW dieitur peret e ln DIRT nn 3 1 4 Introduction iem ee tip ce tdeo v dd tx ede ve en 3 1 2 Sections which must be read and followed in sequence for correct installation 4 1 3 Reference sections once installation has been achieved ssssssssss 4 SECTION 2 UNPACKING AND INITIAL INSPECTION seen nennen 6 2 1 Unpacking the Spectrophotometer sssssssssseseeeenneeen nennen nennen 6 2 2 Unpacking the Computer if appropriate seen 6 2 3 Unpacking the Printer Plotter if appropriate sssseeeeeneen 7 SECTION 3 SAFETY CONSIDERATIONS sse nennen nnne nennen nnne 8 3 1 Satfety PIecautions necem tei dio etatetiet e eto eee dates 8 3 2 Cautions and Warning Statements sssssssssssssssee eene 8 3 3 Impaired Safety Protection ssssssssssssssseseeeeene enne entente 9 3 4 Explanation of Symbols 2 ne et toit Le eee OE eet e aia 9 SECTION 4 LOCATION AND POWER REQUIREMENTS sse 10 4 1 Locating the spectrophotometer esesseeeene eene eene 10 4 2 Connecting the spectrophotometer to
36. e safety of the user 2 Make sure the computer ON OFF switch is in the OFF position and plug the mains cable into the mains socket at the rear of the computer 8 Provided all installation work has been done as detailed in Section 4 3 insert the cable mains plug into a mains outlet provided with a protective earth contact Locating the Printer Plotter if appropriate If there is no requirement to position a printer plotter move on to Section 5 Interconnections Locate the printer plotter adjacent to the other components of the system ensuring that the environment conforms to the environmental requirements detailed in the manufacturer s handbook 14 Issue 1 August 2003 4 6 Nicolet Evolution 500 User Manual Connecting the Printer Plotter to the Mains This section applies to the printer plotter irrespective of whether it is connected to the spectrophotometer or a PC 4 6 4 Earthing Grounding Before any other connection is made the printer must be connected to a protective earth conductor via the three core mains cable see Section 4 6 3 The mains plug must only be inserted into a socket outlet provided with a protective earth contact This protective action must not be negated by the use of an extension cable without a protective conductor WARNING Any interruption of the protective earth conductor inside or outside the instrument is likely to make the instrument dangerous No responsibility will be accepted for any c
37. ect from the mains supply 2 Using a lint free cloth dampened with a weak solution of detergent and water wipe the exterior surface of the instrument as necessary 3 Wipe over with a cloth dampened with plain water 4 Dry the surface with another cloth 10 3 Removal and Replacement of Tungsten Halogen Lamp WARNING Switch off and disconnect the spectrophotometer from the mains and allow the lamp to cool for at least 15 minutes before proceeding 1 Remove the top rear cover by turning each fastener one quarter turn anti clockwise and sliding the cover back and up to remove See Fig 10 1 2 Lift out black metal light shield 3 Hold the Tungsten lamp and pull upwards to remove see Fig 10 2 Note There are variations in the filament height of tungsten lamps from different manufacturers In order to cope with this some lamps supplied may have spacers included These spacers should be fitted under the tungsten lamp holder i e between the holder and the main base casting If subsequently a lamp is supplied without a spacer ensure that any spacer already fitted under the holder is removed 57 Issue 1 August 2003 Nicolet Evolution 500 User Manual CAUTION When fitting the new tungsten halogen lamp avoid handling the silica envelope Finger marks become burnt on and cannot be removed after thelamp is switched on This will affect the output characteristics of the lamp Contamination is best avoided by keeping the lam
38. ells Any of these cells can be used with the SuperSipper 10mm Compact UV Silica Glass 10mm Standard UV Silica Glass 8 1 4 Software VISIONsecurity PC software 9423 UV5 1240E 9423 UV5 1200E 9423 UV5 1210E 9423 UV5 2200E 9423 UV6 2200E 9423 UV5 3200E 9423 UV6 4650E 9423 UV6 4610E 9423 UV6 4400E 9423 UV6 4200E 9423 UV6 4210E 9423 UV6 4220E 9423 168 14601 9423 168 14591 9423 168 14351 9423 168 14341 10 04 0301 Multiple security options Capable of achieving 21 CFR Part 11 and electronic signatures full audit trails and traceability Scanning multiple wavelength ratio quantification and multicomponent analysis applications Scan Absorbance log o A T R 15 4 derivative 100 point peak and position pick X spectra by factor or spectra Fixed Up to 10 wavelengths Normal x or peak height modes Quant Up to 50 standards 3 replicates 4 curve fits single wavelength dual wavelength peak height or factor modes MCA Up to 20 standards 20 components Advanced Results Calculation with built in spreadsheet applies mathematical functions to multiple batches samples or standards WYSIWYG report composer On line Help system Operates with Windows 2000 NT4 or 98 2000 NT4 with a NT 2000 network Includes RS232C cable 32 Issue 1 August 2003 Nicolet Evolution 500 User Manual VISIONsecurity PC software 5 User Licence 10 04 0341 Upgrade to VISIONsecurity fro
39. eter Initialisina Initialise Optics Test Lamps Initialise Monochromator Test Optics Optimise Monochromator iG ul Set Defaults As each stage of the initialisation is successfully completed a yellow pass symbol will appear in the box alongside each test At the end of initialization this window disappears 3 Once the initialisation process has finished it should be possible to observe a live changing status display Oc 0 IF BOTH DISPLAYS CAN BE OBSERVED THEN THE INSTALLATION IS SUCCESSFUL 24 Issue 1 August 2003 Nicolet Evolution 500 User Manual SECTION 7 TROUBLESHOOTING This section offers guidance to the user in the event of operating problems Where possible remedial action is suggested However it deals only with those items of service which can be safely carried out by the operator Any service beyond that detailed should be carried out by a Thermo Electron approved service engineer 7 1 Fault Finding Symptom The instrument fails to respond The front panel LEDS remain off The instrument fails to respond Display is blank The front panel Red LED is on The instrument fails to power up correctly One or more side LEDs remains on See Fig 7 1 The instrument fails to initialize Error 3027 NB Failure to initialize will cause several other consequent errors as well as 3027 The front panel green LED is on but the display is blank Error 3014 Error 3008 Comment an
40. f the available energy down the sample beam Just beyond the beam splitter the beams pass through a modulator This is under software control and permits the detector to see either the sample beam the reference beam or dark There is provision for a Calibration Validation Unit CVU this fits in the sample beam just beyond the modulator Calibration Validation can be performed either by measuring certain frequencies in the emission spectrum of the Mercury Lamp or by using the Tungsten Halogen Lamp and a filter disk within the CVU driven by a motor under software control 39 Issue 1 August 2003 Nicolet Evolution 500 User Manual After passing through the sample compartment the beams are recombined and directed onto the detector Provision is made for either a photodiode or a photomultiplier detector Fig 9 1 Optical diagram of an Evolution 500 spectrophotometer 9 2 2 Electronics All of the power supplies and motor drive circuits are on a PCB mounted below the base casting The power supplies use switching regulators running off a 24V supply derived from a mains transformer A sensing circuit monitors each of the supplies to ensure that they stay within acceptable limits The Tungsten lamp supply has a soft start to prolong lamp life and a sensing circuit to ensure the lamp filament is intact The instrument is controlled by a Motorola 68340 microprocessor This provides an RS232 interface to the outside world and in Local Contro
41. f the carton Caution The instrument weighs 29 Kg 5 Locate in a suitable position see Section 4 2 2 Unpacking the Computer if appropriate Due to the rapid rate of technological improvement Thermo Electron update their list of approved computers and disk operating systems from time to time Please contact your local Thermo Electron Sales Office or agents for further information on currently approved computers 6 Issue 1 August 2003 2 3 Nicolet Evolution 500 User Manual Each computer comprises the following major items Base Unit Keyboard Monitor Mouse Check the contents of the shipment against the delivery note received with the equipment for completeness and possible transport damage If the contents are incomplete or damaged a claim should immediately be filed with the carrier The nearest Thermo Electron Sales Organisation or Agents should also be notified by telephone confirmed in writing of any damage and or items not supplied in order to facilitate repair or replacement Unpacking the Printer Plotter if appropriate Due to the rapid rate of technological improvement Thermo Electron update their list of approved printers plotters from time to time This is particularly appropriate for details of serial printers plotters directly supported by a Standalone Evolution 500 spectrophotometer Please contact your local Thermo Electron Sales Office or agents for further information on currently approved printe
42. for your instrument It has a low current heater which once the lamp has been struck remains on to maintain maximum low drift stability and maximise lamp life The average life is approximately 500 hours The Mercury lamp is an optional accessory A Mercury lamp power supply is fitted to the instrument as standard 9 3 1 Looking after the Lamps Never touch the glass envelope of any lamp This is because fingerprints will be burnt onto the glass and cannot be removed A shorter life and lower output will result Use the Initialise with ON OFF facility The recommended life of a Deuterium lamp is 500 hours and this can be reduced by constant switching therefore it is important that you set the Initialise with ON OFF sequence to suit your requirements This is set on the Local Control software from the SETUP INITIALISE page or from the Commands Set Times menu of the Vision software Keep track of the lamp status and replace before it fails The Local Control software will inform you of the current lamp status i e on or off and the lamp hours used to date via the LAMPS page Do not forget to use the RESET HOURS facility on this page when a lamp has been replaced The VISION software shows the status of the lamps colouring the Status Window icons blue for UV and yellow for Visible A red background will indicate a lamp failure accompanied by an error message Double clicking on either lamp icon brings up a pop up box indicating lamp hours It
43. for several performance variants and a family of accessories The instrument may be controlled by an external computer or by a built in display and keyboard 9 2 1 Optics The optical system is based around an Ebert Monochromator using a 1200 line mm Holographic Grating Slit sub assemblies can be fitted to give variable bandwidth or one of 2 fixed bandwidths The lamphouse is situated in the right hand rear corner of the instrument The airflow in this region is arranged to remove excess heat and ozone to ensure stable lamp output There is provision for three lamps Tungsten for the visible region Deuterium for the UV region and Mercury for wavelength calibration A motor driven mirror M1 under software control selects the lamp to be used The grating is mounted on an approximate sine arm which is moved by a micrometer driven by a 1 8 stepper motor 200 steps rev The micrometer mounting and sliding coupling to the motor are designed to minimise the effects of temperature changes on wavelength accuracy Wavelength accuracy is achieved by software compensation The calibration routine finds 9 predefined emission lines over the wavelength range using Mercury and Deuterium lamps and uses these to characterise the instrument and so calculate the correct motor position for the required wavelength After leaving the monochromator the beam is directed onto a beam splitter to produce the sample and reference beams The beam splitter sends most o
44. ght filter Nal 260nm cut off Stray light filter KCl 200nm cut off NTRM Filter set 1 930 NTRM Filter set 2 1930 NTRM Performance Verification Set Recalibration of NTRM 930 Recalibration of NTRM 1930 Recalibration of NTRM Performance Verification Set Set of 2 sealed potassium dichromate solutions Set of 6 sealed potassium dichromate solutions Recalibration for a set of 2 sealed potassium dichromate cells Recalibration for a set of 6 sealed potassium dichromate cells UV Absorbance filters set of 5 UV Wavelength filter Evolution 500 Hg Lamp Accessory Self Test Filter and attenuator set uncertified 34 9423 179 38091 4013 229 89811 9423 185 03001 4013 229 89851 9423 185 03111 4013 229 89841 9423 UV9 5500E 9423 UV9 5520E 9423 CRM 9300E 9423 CRM 1930E 9423 CRM 9400E 9423 UV9 1760E 9423 UV9 1860E 9423 UV9 1660E 9423 UV9 5100E 9423 UV9 5200E 9423 UV9 5120E 9423 UV9 5220E 9423 UV9 5530E 9423 UV9 5540E 9423 UV9 0023E 9423 UV6 1200E 9423 179 08501 Issue 1 August 2003 Nicolet Evolution 500 User Manual 8 1 8 Optical Accessories Integrating Sphere Powder Cup for Integrating Sphere Variable Angle Specular Reflectance accessory 8 1 9 Output Devices Colour inkjet printer 220 240V Colour inkjet printer 110 130V Laser printer 220 240v Laser printer 110 130v Serial to parallel printer adaptor for Nicolet Evolution 500 8 1 10 Documentation Spare Evolution 500 user manual Sp
45. he mains supply and switch on Allow half an hour for warm up time Lamp hours must be reset by the controlling software See Section 9 9 of the Local Control Software Operating Manual or refer to the Vision Help Text 10 5 Renewal of Fuses All the following fuses are located on the Power Supply Unit and Motor drive Printed Circuit Board except for the Mains fuse A fuse must always be replaced with one of the correct type and value as detailed 60 Issue 1 August 2003 Nicolet Evolution 500 User Manual WARNING Switch off the spectrophotometer and disconnect from the mains before replacing a fuse Note Ifa fuse blows continually this indicates a fault which should be dealt with by a Thermo Electron engineer 10 5 4 Mains Fuse This fuse is located in the mains connection plug on the left hand rear side of the instrument Disconnect the mains cable slide the window up and pull the lever marked FUSE PULL to remove Only the following type of fuse should be used see Fig 10 4 110V operation 7A 2422 086 01435 240V operation 4A 2422 086 01428 10 5 2 Internal Fuses Location PSU and motor drive pcb beneath optical bench Access to the internal fuses 1 Disconnect the mains supply Remove any loose articles from the sample compartment such as cells etc Note If the Sipper accessory is fitted ensure the cell is empty Tip the instrument up and slowly backwards onto its rear end which is designed for that purpose
46. here is always considerable optical attenuation 1A or more in the SAMPLE beam It therefore increases the EHT until the REFERENCE signal is at the optimum value throughout the wavelength range This is useful with highly absorbing accessories where an EHT can be calibrated specifically for the accessory and used only when it is fitted Flat This profile maintains a constant EHT value throughout the wavelength range It is used only when the instrument is operated in EMISSION mode to measure the emission lines of the Deuterium or Mercury lamp eg for checking wavelength calibration When making these measurements the Mode should normally be set to INTENSITY on the SCAN PARAMETERS page 46 Issue 1 August 2003 Nicolet Evolution 500 User Manual Calibration how and why How using Local Control Software Press HOME Press SETUP Select EHT ENTER Use the up down arrow keys to highlight the appropriate table NORMAL or HIGH ABS and ENTER to select Press CALIBRATE Select PROCEED ENTER It is NOT recommended to try calibrating the FLAT EHT profile How using VISION software From the SCAN or FIXED menu bar key COMMAND EHT CALIBRATION NORMAL or HIGH ABS Why The appropriate EHT profile Normal or High Abs should be calibrated if there has been any significant change in the energy as seen by the photomultiplier Possible causes are Lamp energy decayed since new to below 7596 CHECK In Local Control Software click
47. ibed procedures which detail the expected responses for each type of system SECTIONS 8 10 Once a working system has been established read these sections to establish a detailed knowledge of the system capabilities to maintain the initial system performance and educate on upgrade capability SECTION 7 Troubleshoot any problems found with the system using the procedures in this section Issue 1 August 2003 1 2 1 3 Nicolet Evolution 500 User Manual Sections which must be read and followed in sequence for correct installation 1 2 1 SECTION 1 Overview A brief summary of the structure of the manual together with a flow chart of the installation process 1 2 2 SECTION 2 Unpacking and Initial Inspection of the System This section deals with the initial unpacking and inspection of all potential components of the basic system i e spectrophotometer computer printer and plotter Installation of any supplied accessories is covered by their own respective manual 1 2 3 SECTION 3 Safety considerations This section details the basic safety issues that must be considered when installing a Nicolet Evolution 500 spectrophotometer system 1 2 4 SECTION 4 Location and Power requirements This section deals with the correct location of all potential components of the basic System i e spectrophotometer computer printer and plotter Installation of any supplied accessories is cove
48. l variants runs the user interface on the LCD display and Keyboard The main memory is made up of 512K words of flash EPROMS These can be erased and re programmed in circuit This means that software upgrades can be easily performed either from the instrument disk drive or from a PC via the RS232 interface The start up and code for programming the flash memory is in a 64K word boot sector of the flash EPROMS The system RAM is in two parts 256K words of volatile RAM and 128K Bytes of battery backed RAM Calibration parameters and serial number are stored in a 64 word EEPROM 40 Issue 1 August 2003 9 3 Nicolet Evolution 500 User Manual The 68340 has two DMA channels one of these is used for the floppy disc interface and the other is used to transfer commands to the wavelength drive and modulator drive circuits Three 68230 peripheral chips provide the input output lines used to control the optical system and interface to the accessories Lamps The Evolution 500 uses two sources to cover the wavelength range specified A Tungsten halogen lamp provides the visible radiation and a Deuterium Arc lamp provides the UV radiation In addition a Mercury lamp can be fitted for checking wavelength accuracy or recalibrating the instrument Your instrument uses a 12V 35W two pin plug in Tungsten lamp which has an average life of approximately 1000 hours The UV radiation source is a Deuterium Arc lamp with an inline connector prealigned
49. lett Packard HP Deskjet 400 and 690C printers Hewlett Packard HP Paintjet 3630A printer Hewlett Packard HP Plotter Printers not on the above list that claim Epson 9 pin 24 pin ESC P or HP PCL Programming Control Language Level 3 compatibility should work with the instrument but are not guaranteed to do so and are therefore not supported If in doubt contact your local Thermo Electron approved Customer Support Organisation Note Printers designed to work only in a Windows environment are not compatible with Local Control Software Depending on the printer plotter it may be fitted with both a parallel and serial connector The serial connector consists of two horizontal rows of pins 13 on the top and 12 on the bottom The parallel connector is a 36 way connector having 18 connecting pins in each row Only an appropriate mating connector can be fitted to either connector For use directly with a Nicolet Evolution 500 Spectrophotometer the printer plotter must have a serial interface for connecting it directly to the Spectrophotometer This may involve setting the value of a number of parameters see printer plotter handbook The serial printer parameters required are shown in Table 5 1 Alternatively this capability may be achieved using a parallel to serial converter For use with a computer the printer parallel interface connects directly to the parallel interface connector LPT1 on the computer base unit Section 5 2 Table 5 1
50. m an earlier VISION version 10 04 0331 VISIONpro PC software 10 04 0101 Scanning multiple wavelength ratio quantification and multicomponent analysis applications Scan Absorbance log o A T R 15 4 derivative 100 point peak and position pick X spectra by factor or spectra Fixed Up to 10 wavelengths Normal x or peak height modes Quant Up to 50 standards 3 replicates 4 curve fits single wavelength dual wavelength peak height or factor modes MCA Up to 20 standards 20 components Advanced Results Calculation with built in spreadsheet applies mathematical functions to multiple batches samples or standards WYSIWYG report composer Security options Method and results storage Operates under Windows 98 2000 or NT Multiple language support Includes RS232C cable VISIONpro PC Software 5 User licence 10 04 0121 Upgrade to VISIONpro from an earlier VISON version 10 04 0121 VISION ife PC software option 10 04 0201 Rate Advanced Kinetics and DNA melting analysis Requires VISIONpro or VISIONsecurity Upgrade to VISION ife from an earlier VISION Rate version 10 04 0221 Requires VISIONpro or VISIONsecurity UVCalc Bio for Local Control Systems 9423 UV8 7050E UVCalc Aqua for Local Control Systems 9423 UV8 7100E 8 1 5 Computers Pentium PC o s Windows NT 220 240V 9423 UV7 0500E Pentium PC o s Windows NT 110 130V 9423 UV7 0510E Pentium PC o s Windows 2000 220 240V 9423 UV7 0600E Pentium PC o s
51. mbly Transformer assembly 55 Issue 1 August 2003 Nicolet Evolution 500 User Manual 9 14 Evolution 500 Recommended Service Intervals EVERY 500 HOURS OR SIX MONTHS Change the Deuterium Lamp Re calibrate the EHT Rerun the Default Baseline Check the Lamp Change mirror for contamination and clean if necessary Clean all sample compartment and external surfaces Perform the following checks if these are not already being done on a regular basis Wavelength Accuracy re calibrate if required Absorbance Accuracy Baseline Flatness Noise Stray Light EVERY 1000 HOURS OR 12 MONTHS Check calibration expiry dates on CVU units filters etc Return for re calibration if necessary Change the Tungsten Lamp Change the Deuterium Lamp Re calibrate the EHT Rerun the Default Baseline Check the Lamp Change mirror for contamination and clean if necessary Clean all sample compartment and external surfaces Lubricate the micrometer drive with Mobil Vactra No 2 oil Section 5 5 1 UV Series Service Manual Perform the following checks if these are not already being done on a regular basis Wavelength Accuracy re calibrate if required Absorbance Accuracy Baseline Flatness Noise Stray Light EVERY 2000 HOURS OR 24 MONTHS Check mains amp connection leads for damage solvent attack etc Check calibration expiry dates on CVU units filters etc Return for re calibration if necessary Change the Tungste
52. mful to the skin and eyes Always view the lamp through protective glasses that will absorb UV radiation Avoid looking directly at the Deuterium arc Do not expose the skin to direct or reflected UV radiation Set the power switch to off and disconnect the spectrophotometer from the mains supply Remove the top rear cover by turning each fastener one quarter turn anti clockwise and sliding the cover back and up to remove See Fig 10 1 Make sure the lamp has cooled Disconnect the lamp at the inline connector see Fig 10 3 Using the key provided loosen the three locating screws rotate lamp assembly anti clockwise and lift lamp out CAUTION When fitting the new deuterium lamp avoid handling the silica envelope Finger marks become burnt on and cannot be removed after the lamp is switched on This can affect the output characteristics of the lamp Handle only the base of the lamp or the mounting plate If the silica envelope does become contaminated clean with a degreasing solvent such as acetone propanone before the lamp is switched on Take the new lamp handling it by the base or mounting plate or through a polythene bag or paper shield Identify the notch in the mounting plate Locate the lamp such that the notch points towards the lamp change mirror Tighten locating screws with the key provided Remove protective wrapping Re connect the new lamp at the inline connector Refit top cover Reconnect the spectrophotometer to t
53. n Lamp Change the Deuterium Lamp Re calibrate the EHT Rerun the Default Baseline Check the Lamp Change mirror for contamination and clean if necessary Clean all sample compartment amp external surfaces Lubricate the micrometer drive with Mobil Vactra No 2 oil Section 5 5 1 UV Series Service Manual Perform the following checks if these are not already being done on a regular basis Wavelength Accuracy re calibrate if required Absorbance Accuracy Baseline Flatness Noise Stray Light 56 Issue 1 August 2003 Nicolet Evolution 500 User Manual SECTION 10 MAINTENANCE The information given in this section deals only with those parts of maintenance or service which can be safely carried out by the user Work other than that detailed should be carried out by a service engineer 10 1 Routine Maintenance Very little maintenance is required to keep the spectrophotometer in good working condition The interior should be kept as dust free as possible and the sample compartment cleaned regularly wipe off spilt chemicals immediately The sample compartment has a drain facility to prevent excessive spillage causing damage to the electronics a drain tube can be attached for collection by an appropriate vessel 10 2 Cleaning Instrument Exterior The exterior of the instrument can be cleaned periodically as follows CAUTION Do not allow moisture to leak into the instrument 1 Switch off the Spectrophotometer and disconn
54. ngth High energy at the detector requires no gain and therefore a short integration time low energy requires high gain and therefore a longer integration time This is what Intelliscan does by intelligently varying the integration time at each data point of the scan depending on the amount of light hitting the detector as measured by the gain required to fill the ADC 49 Issue 1 August 2003 9 6 Nicolet Evolution 500 User Manual When Should Intelliscan be Used Intelliscan can be used in any application to improve the quality of the result However where the most benefit is seen is where high definition is required from a sample which has strongly absorbing peaks in both the UV and Visible regions Wavelength Accuracy and Recalibration Wavelength accuracy is the most important performance criteria of a spectrophotometer In the Evolution 500 a combination of stepping monochromator and software compensation equation based on 9 Mercury and Deuterium emission lines should give years of trouble free service However there are circumstances where it may be necessary to recalibrate your instrument Factors Affecting Wavelength Accuracy Physical Shock Accuracy may be affected if the spectrophotometer is jolted violently dropped or subjected to severe or prolonged vibration Temperature Problems may be found if the operating temperature is significantly different from the temperature at which the spectrophotometer was last calibrate
55. nment currently in use Up to 20 discrete values are held in memory by the instrument and are non volatile The last 20 wavelengths measured are always held 9 5 1 4 How baselines are used DEFAULT BASELINE This is factory set and specifically should only be rerun if 1 The wavelength is recalibrated 2 The optics are cleaned or changed 3 After EHT CALIBRATION see Section 9 5 2 THIS PROCESS WILL TAKE APPROX 60 MINUTES WITH THE DEFAULT BASELINE CORRECTLY SET THE INSTRUMENT BECOMES A CLASSICAL DOUBLE BEAM i e BOTH BEAMS BALANCED AND ANY DIFFERENCE RECORDED AS A MEASUREMENT NO ADDITIONAL BASE LINES ARE REQUIRED FOR ROUTINE MEASUREMENT USER BASELINES SEE BELOW HOWEVER SHOULD BE USED FOR ABSOLUTE ACCURACY USER BASELINE With Single beam instruments a baseline is performed usually immediately before measuring the sample Whilst this could be conceived to be a disadvantage slowing down the sample measurement process in the Evolution 500 this procedure is fast with the added benefit that the user baseline effectively compensates for any minor changes in the background environment occurring after performing the Default Baseline The user therefore gets a combination of the best of both instrument types i e double beam operation with accurate baseline correction ZERO When this is used by any of the controlling software packages the wavelength value used is stored in volatile memory and the instrument is ze
56. nt or other property WARNING Indicates a potential danger that requires correct procedures or practices in order to prevent injury to personnel 8 Issue 1 August 2003 3 3 3 4 Nicolet Evolution 500 User Manual Impaired Safety Protection Whenever it is likely that safety protection has been impaired the instrument and or accessory must be made inoperative and secured against any unintended operation The matter should then be referred to the appropriate servicing authority Safety protection is likely to be impaired if for example the instrument fails to perform the intended measurements or shows visible damage Explanation of Symbols yellow black To protect the instrument from damage the red white operator must refer to an explanation in the Users Manual D white black Protective earth ground terminal 9 Issue 1 August 2003 Nicolet Evolution 500 User Manual SECTION 4 LOCATION AND POWER REQUIREMENTS This section deals with the correct location of all potential components of the basic system i e spectrophotometer computer printer and plotter Installation of any supplied accessories is covered by its own respective manual Once the components are suitably located move on to Section 5 for detailed interconnection instructions 4 1 Locating the spectrophotometer The system is designed for use on a normal desk or bench wide enough to ensure that all four feet sit on the surface The mounting
57. ntrol Software Manual E3072 3 EEPROM FAILURE An error was reported when writing data to the configuration EEPROM Repeat the command and call Customer Support if the problem persists E3074 DARK GAIN LOW The dark signal is too high it is over range at minimum gain Ensure that there are no light leaks then perform an EHT calibration on the system See Section 9 3 of the Local Control Software Manual and call Customer Support if the problem persists E3075 DARK GAIN HIGH The dark signal is smaller than expected This could be due to the EHT being too low Perform an EHT calibration on the system See Section 9 3 of the Local Control Software Manual and call Customer Support if the problem persists 30 Issue 1 August 2003 Nicolet Evolution 500 User Manual SECTION 8 SYSTEM DESCRIPTION The system comprises three main components a Nicolet Evolution 500 spectrophotometer a controlling device and an output device The controlling device is either a built in LCD and Keypad running Local Control software or an external PC running VISIONpro or VISIONsecurity software 8 1 System Composition 8 1 1 Nicolet Evolution 500 Spectrophotometers Evolution 500 PC Control with VISIONsecurity software 10 50 0101 Evolution 500 true double beam scanning UV visible spectrophotometer single photomultiplier detector Variable Bandpass 0 2 to 4nm Wavelength range 190 900nm Self optimizing sealed and quartz coated optical system Tw
58. nual SECTION 2 UNPACKING AND INITIAL INSPECTION This section deals with the initial unpacking and inspection of all potential components of the basic system i e spectrophotometer computer printer and plotter Installation of any supplied accessories is covered by its own respective manual Once the components are unpacked and checked OK move on to Section 3 for Safety Instructions 2 1 Unpacking the Spectrophotometer 1 Check the contents of the shipment against the delivery note received with the equipment in the spectrophotometer box for completeness and possible transport damage Note If the contents are incomplete or damaged a claim should immediately be filed with the carrier The local Sales organisation or nearest Agent should be notified by telephone confirmed in writing of any damaged or missing items in order to facilitate replacement or repair 2 Open the box containing the spectrophotometer and read the enclosed yellow card If you have purchased the Validator package stop at this point and continue the Installation using the Validator Log supplied 3 Remove to a safe location the following e The User manual and if the spectrophotometer is a Standalone Evolution 500 the Local Control Software Operating Manual e Certificates of Commissioning and Compliance to Specification e Pack of spare fuses etc 4 Remove the top of the carton and pick up the instrument via the recesses in the bottom o
59. o 1 50mm pathlength cell holders Computer ready with built in RS232C port Built in initialization and error diagnostics VISIONsecurity PC software Scanning Quantification Multiple wavelength Ratio and Multicomponent Analysis Multiple security options capability of achieving 21 CFR Part 11 and electronic signatures full audit trails and data traceability Method and results storage Advanced results calculation Multiple language support Rate Advanced Kinetics Analysis and DNA melting optional Instrument verification and calibration optional wide range of accessories optional 29kg 248x552x584mm 115 230v and 60 50Hz Evolution 500 PC Control with VISIONpro software 10 50 0201 Evolution 500 true double beam scanning UV visible spectrophotometer single photomultiplier detector Variable Bandpass 0 2 to 4nm Wavelength range 190 900nm Self optimizing sealed and quartz coated optical system Two 1 50mm pathlength cell holders Computer ready with built in RS232C port Built in initialization and error diagnostics VISIONpro PC software Scanning Quantification Multiple wavelength Ratio and Multicomponent Analysis Security options Method and results storage Advanced results calculation Multiple language support Rate Advanced Kinetics Analysis and DNA melting optional Instrument verification and calibration optional wide range of accessories optional 29kg 248x552x584mm 115 230v and 60 50Hz Ev
60. olution 500 Local Control 10 50 0301 Evolution 500 true double beam scanning UV visible spectrophotometer single photomultiplier detector Variable Bandpass 0 2 to 4nm Wavelength range 190 900nm Self optimizing sealed and quartz coated optical system Two 1 50mm pathlength cell holders Computer ready with built in RS232C port Built in initialization and error diagnostics Local Control software Scanning Quantification Multiple wavelength Ratio and Multicomponent kinetics and life science analysis Multiple access level security authorization Advanced results calculation Multiple language support Standard floppy disk drive method and results storage Instrument verification and 31 Issue 1 August 2003 Nicolet Evolution 500 User Manual calibration optional Wide choice of PC software optional Wide range of accessories optional 29kg 248x552x584mm 115 230v and 60 50Hz 8 1 2 Evolution 500 Cell Holders 100mm Pathlength rectangular cell holder Variable Path Length Cell Holder 100mm Pathlength cylindrical cell holder Thermostatted single cell holder 8 1 3 Sample Handling Accessories 8 Cell Programmer Multi Function Cell Holder for Above Cetac ASX510 360 position sampler for Vision Auto Cetac ASX510 160 position sampler for Vision Auto Gilson 222XL Autosampler for Vision Auto SuperSipper Requires tubing pack and flowcell SuperSipper Standard Tubing Pack SuperSipper Acid Tubing Pack Flowc
61. ommand while in standby mode The spectrophotometer requires the WAKEUP command from the Vision Software before it can action further commands See Section 9 9 of this Manual 28 Issue 1 August 2003 Nicolet Evolution 500 User Manual E3053 DAC OFFSET During optical initialization it was not possible to null the offsets on the pre amplifier signal Check that the covers are fitted correctly and that the sample compartment is light tight then try again If the fault persists call Customer Support E3054 CELL PROGRAMMER The cell programmer has stalled Check that nothing is jamming the transport mechanism E3055 CELL PROGRAMMER TIMEOUT The cell programmer has not moved to the required cell position in the time allocated Check that nothing is jamming the transport mechanism E3056 NOT INITIALISED The spectrophotometer has become un initialised Try switching the instrument off then on again and allow it to re initialise Call Customer Support if the problem persists E3058 DAC ERROR Offsets cannot be correctly nulled during gain calibration Perform a Lifetime Initialisation See Section 9 3 1 of this Manual If the problem persists call Customer Support E3059 GAIN ERROR The instrument gains are not ascending from minimum to maximum as expected This can occur during a gain calibration if the starting conditions are not correct or if there is a light leak Check for light leaks then perform a lifetime initialisation See Secti
62. on 9 3 1 of this Manual and repeat the gain calibration If the problem persists call Customer Support E3060 DEFAULT BASELINE There is no valid baseline in the non volatile memory Run a new default baseline using the appropriate software commands See Section 9 7 in the Local Control Software Manual E3062 EHT INHIBIT The EHT supply is inhibited This is probably due to the sample compartment lid being opened See Section 9 4 4 of this Manual Ensure that the Sample Compartment lid is properly closed E3064 EHT CALIBRATION ERROR The EHT calibration failed This may be because the lid was opened Please ensure that the lid is properly closed repeat the EHT calibration See Section 9 3 of the Local Control Software Manual and call Customer Support if the fault persists E3065 STORED EHT VALUE PROBLEM The checksum of one or other or both the EHT data stores is incorrect Please run an EHT calibration for both the Normal and High Energy tables See section 9 5 2 of this Manual Section 9 3 of the Local Control Software Manual Contact Customer Support if the problem persists 29 Issue 1 August 2003 Nicolet Evolution 500 User Manual E3066 MONOCHROMATOR ERROR The monochromator has been requested to move a number of steps that are out of range Please re run a Mercury Wavelength Calibration See section 9 6 1 of the Local Control Software Manual and call Customer Support if the problem persists E3067 MONOCHROMATOR ERROR
63. ons who have been on an approved Thermo Electron training course and who are fully familiar with the precautions necessary should undertake this procedure Standby and Wakeup When using any spectrophotometer the Wake Up amp Standby Times x most accurate results will be obtained only when the temperature of the instrument is in Wake Up Standby equilibrium with its surroundings The FE e f2dH recommended warm up time required to Monday 3 00 17 30 reach this equilibrium is approximately 30 Tuesday 9 00 47 30 minutes Ji Wednesday 9 00 17 30 In a busy laboratory this may be regarded as Trim 30 1730 an unacceptable waste of time so to DUAE ra overcome this inconvenience the VISION Friday 3 00 17 30 software offers the two facilities of Standby Saturday 0 00 0 00 and Wakeup which can be used in Sunday 0 00 0 00 conjunction with the Nicolet Evolution 500 These enable the instrument to be warmed up and ready for use at the start of every day i Cancel Defaults As you can see from Fig 9 4 it is possible set the daily times at which the instrument will automatically start up and initialise before the start of the working day and also go back to sleep in the Standby mode at the end of the day IV Switch on D2 lamp after wake up Fig 9 4 Vision Wake Up and Standby Window Should you wish to begin working immediately in the UV area of the spectrum there is the added facility of being a
64. onsequences of failure to ensure adequate earthing grounding 4 6 2 Mains Voltage Setting Check that your printer plotter is suitable for the local mains supply It is not possible for the user to alter the mains voltage setting Note The Epson printer is available in 240V 220V or 110V versions 4 6 3 Printer Plotter Mains Cable Connection If you have been supplied with a mains cable with no plug fitted or if the fitted plug is unsuitable refer to operation 1 and connect a suitable plug When fitted carry out operations 2 and 3 If the mains cable has been supplied with a suitable plug fitted carry out operations 2 and 3 1 Fita suitable 3 pin plug to the free end of the cable connecting individual leads as follows Plug pins European N American Code Code LIVE L Brown Black NEUTRAL N Blue White EARTH GROUND E Green Yellow Green A good earth is essential both for satisfactory operation of the printer and the safety of the user 2 Make sure the Power ON OFF switch is in the OFF position Plug the mains cable into the POWER socket at the rear of the printer 3 Provided all installation work has been done as detailed in this section and in the printer manual insert the mains plug into a socket outlet provided with a protective earth contact 15 Issue 1 August 2003 Nicolet Evolution 500 User Manual SECTION 5 SYSTEM INTERCONNECTIONS This section covers the complete Nicolet Evolution 500
65. p is used over the whole range On the Evolution 500 it is possible to select the tungsten deuterium and mercury lamps in this way The interaction between the lamp switching and the EHT tables is shown in the table below 45 Issue 1 August 2003 Nicolet Evolution 500 User Manual now uo s BE Absorbance De Auto 315 340 nm BER signal at NL signal atall Minimum NEC MS Tungsten all wavelengths wavelengths with part of Normal table accessory present Tungsten Constant signal from Constant signal from Minimum of Tungsten lamp change to lamp change to part of Normal table 900nm constant 900nm constant EHT EHT from 190 nm to from 190 nm to the the lamp change lamp change values values taken from taken from HAA Normal table table Deuterium Constant signal from Constant signal from Minimum of Deuterium 190 nm to lamp 190 nm to lamp part of Normal table change constant change constant EHT above lamp EHT above lamp change values change values taken taken from Normal from HAA table table Mercury Flat Flat Set during peaking up of lamp Notes 1 The EHT is scaled depending on the bandwidth 2 The EHT is scaled depending on the bandwidth to achieve comparable peak heights at all bandwidths To summarise there are three different EHT profiles Normal This profile adjusts the SAMPLE signal level to the optimum value throughout the wavelength range High Absorbance This profile assumes that t
66. p within its protective sleeve or polythene bag with the pins protruding until the lamp has been installed in its holder Alternatively a paper shield may be wrapped around the lamp envelope If the silica envelope does become contaminated clean with a degreasing solvent such as acetone propanone before the lamp is switched on 4 Use the new lamp s protective sleeve polythene bag or a piece of tissue paper wrapped around the lamp and insert the pins into the socket When the lamp is in place remove the protective sleeve polythene bag or paper shield 5 Replace black metal light shield 6 Replace the top cover 7 Reconnect the spectrophotometer to the mains supply and switch on 8 Allow half an hour for warm up time 9 Lamp hours must be reset from the controlling software See the Local Control Software Operating Manual or refer to the Vision Help Text Fig 10 1 Rear Cover removal 58 Issue 1 August 2003 Nicolet Evolution 500 User Manual Fig 10 2 Tungsten Lamp replacement Inline connector Lamp change mirror Locating screws Fig 10 3 Deuterium Lamp replacement 59 Issue 1 August 2003 Nicolet Evolution 500 User Manual 10 4 Removal and Replacement of Deuterium Lamp WARNINGS 1 Switch off and disconnect the spectrophotometer from the 1 2 3 mains supply and allow the lamp to cool for at least 15 minutes before proceeding 2 UV radiation from a Deuterium lamp can be har
67. red by its own respective manual 1 2 5 SECTION 5 System interconnections This section covers the complete Nicolet Evolution 500 system interconnections 1 2 6 SECTION 6 System Power Up This section details the application of power to a correctly configured system and the system responses the user should expect if everything is OK 1 227 SECTION 7 Troubleshooting This section is intended to guide the user towards taking the appropriate action required to reduce the instrument downtime to a minimum It deals only with those items of service which can be safely carried out by the operator Reference sections once installation has been achieved 1 3 1 SECTION 8 System Description This section gives basic system descriptions part numbers etc 1 3 2 SECTION 9 Getting the best from your Nicolet Evolution 500 Instrument The Nicolet Evolution 500 double beam scanning UV Visible spectrophotometer offers one of the best value for money systems on the market It is the purpose of this section to help you get the best from your Nicolet Evolution 500 spectrophotometer 4 Issue 1 August 2003 Nicolet Evolution 500 User Manual 1 3 3 SECTION 10 Maintenance The information given in this section deals only with those parts of maintenance or service which can be safely carried out by the user Work other than that detailed should be carried out by a service engineer 5 Issue 1 August 2003 Nicolet Evolution 500 User Ma
68. roed Returning to any stored wavelength bandwidth combination will re establish the stored zero value 9 5 1 5 How to get the best results These are achieved if a User baseline or zero is run immediately before the first sample as the instrument will always use a User baseline in preference to the Default 44 Issue 1 August 2003 Nicolet Evolution 500 User Manual 95 2 EHT Explanation what is a profile The light energy received by the detector varies very considerably Many components e g lamps mirrors gratings etc have efficiencies which vary with the wavelength used Therefore for a fixed bandwidth the energy at the detector varies by about 100 1 over the range from 190 to 900nm In addition varying the bandwidth has a large effect on the energy which is proportional to the square of the bandwidth The change of energy from 4nm bandwidth to 0 2nm bandwidth is 400 1 Thus the total energy variation is 40 000 1 For a photomultiplier Tube PMT to operate it must have a voltage applied to it This is typically a few hundred volts up to 1200 V This voltage sets the gain of the PMT To get the best performance from the signal processing electronics the detector signals need to be kept as large as possible Evolution 500 instruments optimise the signal level by varying the high voltage EHT supply to the photomultiplier Electrons emitted by photons striking the photo cathode are multiplied by nine stages of gain at the in
69. rs Check the contents of the shipment against the delivery note received with the equipment for completeness and possible transport damage Unpack the printer plotter according to the manufacturer s instructions found in the printer plotter box If the contents are incomplete or damaged a claim should immediately be filed with the carrier The nearest Thermo Electron Sales Organisation or Agents should also be notified by telephone confirmed in writing of any damage and or items not supplied in order to facilitate repair or replacement 7 Issue 1 August 2003 Nicolet Evolution 500 User Manual SECTION 3 SAFETY CONSIDERATIONS This section details the basic safety issues that must be considered when installing a Nicolet Evolution 500 spectrophotometer system Once this section has been read move on to Section 4 and locate the components ready for interconnection Read this page carefully before installing and using the instrument and its accessories The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT 1974 The instrument and accessories described in this manual are designed to be used by properly trained personnel only Adjustment maintenance and repair of exposed equipment must be carried out only by qualified personnel who are aware of the hazards involved Where indicated in the relevant manual certain maintenance processes may be carried out by the user who must be fully a
70. s are checked for correctness Self test If a Self Test accessory or CVU accessory is fitted the built in filters are used to check for approximate absorbance accuracy at 0A 1A 2A and 3A Deuterium lamp If selected to initialise with Deuterium the lamp heaters are applied When warm the lamp is struck After a stabilising delay the heaters are turned off The lamp current is checked The monochromator is driven to 250nm The lamp select mirror is scanned to find the exact position of the Deuterium lamp 9 5 3 1 Life time Initialisation This goes through the same sequence of steps as the normal optical initialisation but searches through a wider range when it is finding the positions of the lamps and monochromator This life time initialisation which takes longer to run is necessary after lamps have been changed any optical components have been changed or if the processor pcb which holds the non volatile memory containing the positions has been changed 9 5 4 Scan Speed When scanning a sample the aim should be to scan the selected range as quickly as possible without any loss of accuracy for either wavelength or absorbance The optimum scan speed for any sample depends on the interaction of a number of complex factors For example the number and sharpness of peaks the wavelength range of the scan the maximum absorbance of the sample the required noise performance the data resolution or interval between readings and
71. s not been possible to find the peak position of the lamp or zero order position of the monochromator Therefore the instrument cannot initialize Check that nothing has been left in either the sample beam or the reference beam Try restarting the instrument before calling Customer Support E3046 CLOCK FAULT Calendar clock chip appears to have stopped running Try re setting a new time If unsuccessful call Customer Support E3047 CALIBRATION FAULT Calibration parameters retrieved from the EEPROM are out of range A mercury lamp calibration is required See Local Control Software Manual Section 9 6 E3048 CALIBRATION FAULT A calibration line was not found during a calibration Try again If problem persists call Customer Support E3049 CALIBRATION FAULT The calibration coefficients do not fit the actual measured data Run mercury lamp calibration again See Local Control Software Manual Section 9 6 If the problem persists call Customer Support E3050 CALIBRATION FAULT The calibration coefficients were not stored in the EEPROM correctly Try running the mercury lamp calibration again See Local Control Software Manual Section 9 6 If the problem persists call Customer Support E3051 CALIBRATION FAULT The wavelength calibration failed Try running calibration procedure again See Local Control Software Manual Section 9 6 If the problem persists call Customer Support E3052 STANDBY MODE The spectrophotometer has received a new c
72. section 4 2 3 If the selection and or fuse is not correct proceed as in 2 Make sure that only fuses of the required current rating and specified type are used for renewal The use of repaired fuses and or the short circuiting of fuse holders will invalidate any warranty 2 Unplug the mains cable if fitted from the socket on the side panel Slide the window up 3 Rotate the FUSE PULL lever downwards and remove the fuse Check the fuse This must be AA 2422 086 01428 for 220 240V mains supply 7A semi delay 2422 086 01435 for 100 120V mains supply 4 Pull out the selector card Fig 4 1 using a small tool in the removal hole or use a small pair of pliers 5 Turn the card to position the desired voltage on the top left side of the card Fig 4 1 Push the card firmly into the card slot 6 Rotate the FUSE PULL lever upwards and push the correct value fuse see 3 into the clips 7 Refer to Section 4 2 3 before plugging in the mains cable 11 Issue 1 August 2003 Nicolet Evolution 500 User Manual Fig 4 1 Mains connector 4 2 3 Mains Cable Connection If your mains cable is fitted with a suitable mains plug carry out operations 3 and 4 below If you have been supplied with a mains cable with no plug fitted or the fitted plug is unsuitable for your environment carry out operations 1 to 4 below 1 If a plug is fitted remove it 2 Fita suitable 3 pin plug to the free end of th
73. sten lamp has failed the deuterium lamp is peaked Optimize monochromator Finds the zero order peak Set defaults Switches on and peaks up deuterium lamp if D lamp flag is set Tests slits Tests absorbance filters if self test fitted Drives to default wavelength Completion of Initialization Once all tests have been complete the display shown below will appear briefly The instrument will then switch automatically to the Home page Completed initialization page Home page 19 01 98 EVOLUTION 500 v7 00 ae 98 EVOLUTION 500 v7 00 a 11 35 SERIAL No TOURS SERIAL No 100661 HOME SPECTROMETER INITIALISING SCAN Y INITIALISE OPTICS FIXED Y TEST W LAMP QUANT Y INITIALISE MONOCHROMATOR RATE Y TEST OPTICS MCA Y OPTIMISE MONOCHROMATOR LIBRARY YSET DEFAULTS PLEASE WAIT INSTRUMENT HOURS 12345 CAL ACCESS VAL ORIES LAMPS SETUP REMOTE APPEARANCE OF BOTH THESE PAGES MEANS THAT THE INSTALLATION IS SUCCESSFUL 23 Issue 1 August 2003 6 2 Nicolet Evolution 500 User Manual Powering up a PC Controlled system using VISION software It is assumed that the VISION software has been installed following the procedures specified in the User documentation 1 Turn on the PC and start up VISION by double clicking on the VISION icon on the Windows desktop 2 Now turn on the Evolution 500 Spectrophotometer the window displayed below should appear Spectrom
74. termediate dynodes before reaching the anode The overall gain is approximately proportional to the seventh power of the voltage Thus doubling the photomultiplier supply voltage will increase the gain by 27 128 Tripling the voltage will increase the gain by 37 2187 Four times the voltage increases the gain by 47 16 384 etc EHT profiles The Evolution 500 has two EHT tables which are used to compensate for variations in energy by changing the voltage applied to the PMT during routine operation One is set up for normal operation NORMAL and the other HIGH ABSORBANCE is used when an accessory which absorbs a significant amount of light is present There is also a series of Flat tables which simply apply a constant voltage to the PMT for use when scanning for emission peaks On variable bandwidth instruments because the amount of light changes with bandwidth the EHT has to be scaled appropriately This is performed when automatic changeover is selected and when the mercury lamp is being scanned In all other situations the scaling is not used to ensure that e g deuterium lines can be scanned satisfactorily In addition to the possibilities for changing the EHT table the Evolution 500 also allows for a number of possibilities when selecting lamps In normal operation the instrument switches automatically between the tungsten and deuterium lamps at the requested lamp change wavelength This switching can be over ridden so that only one lam
75. the type of detector to name but a few The stepping monochromator design and advanced signal processing of the Evolution 500 instruments mean that wavelength accuracy is independent of scan speed therefore the main consideration in selecting scan speed will be the acceptable noise level on the spectrum The following list gives the background information relevant to noise and scan speed 48 Issue 1 August 2003 Nicolet Evolution 500 User Manual 1 The amount of energy or signal reaching the detector at any wavelength depends on the source energy and the sample absorption at that wavelength 2 Noise is proportional to the square root of the signal therefore the amount of noise increases as a proportion of the signal as the level of signal falls consequently the amount of noise varies across the scan 3 Where high absorbance coincides with low source energy the noise due to the signal is markedly greater than where high absorbance coincides with high Source energy 4 Noise can be reduced by increasing the time for which a data point is measured integration time 5 Noise can be made constant across the scan by varying the integration time at each data point 9 5 4 1 Standard The Standard scan speed option allows the setting of fixed scan speeds The maximum rate at which the spectrophotometer can scan is 1200 data points per minute This equates to 2400 nm min at 2nm data interval or 120nm min at 0 1nm data interval
76. ting the computer to the Mains 4 4 4 Earthing Grounding Before making any connections the computer must be connected to a protective earth conductor via the three core mains cable supplied The mains connector must only be inserted into a mains outlet provided with a protective earth contact This must not be negated by the use of an extension lead without a protective earth contact WARNING Any interruption of the protective earth conductor inside or outside the instrument is likely to make the instrument dangerous No responsibility will be accepted for any consequences of failure to ensure adequate earthing grounding 4 4 2 Mains Voltage Setting Check that your computer is suitable for the mains supply it is to be used on It is not possible to alter the mains voltage setting of the computer 4 4 3 Mains Cable Connection If you have been supplied with a mains cable with no plug fitted or if the fitted plug is unsuitable refer to operation 1 and connect a suitable plug When fitted carry out operations 2 and 3 If the mains cable has been supplied with a suitable plug fitted carry out operations 2 and 3 1 Fita suitable 3 pin plug to the free end of the cable connecting the individual leads as follows Plug pins European N American Code Code LIVE L Brown Black NEUTRAL N Blue White EARTH GROUND E Green Yellow Green A good earth is essential both for satisfactory operation of the instrument and th
77. trical equipment designed for use within certain voltage limits Low voltage directive Such marking indicates that this Thermo Electron system meets or exceeds the technical standards listed in the Declaration of Conformance DECLARATION OF CONFORMITY according to ISO IEC Guide 22 and EN 45 014 Manufacturer s Name Thermo Electron Manufacturer s Address Mercers Row Cambridge CB5 8HY UK declares that the product s Product name UV Visible Spectrophotometers Model Number s Nicolet Evolution 500 conforms to the following Product Specifications Safety BS EN 61010 1 1993 Parti General Requirements EMC EN 50 082 1 Light Industrial Generic Immunity Standard IEC 801 2 Electrostatic Discharge 8k V 15 kV IEC 801 3 RF Field Immunity 3 V m 10 V m IEC 801 4 Electrical Fast Transients EN 50 081 1 Light Industrial Generic Emission Standard EN 55 022 Class B ITE Radio Frequency Emission FCC 47CFR Part 15 B Class A Supplementary Information The documentation relating to this declaration is on file The products herewith comply with the requirements of the Low Voltage Directive 73 23 ECC and the EMC Directive 89 336 ECC 1 August 2003 G Smart Cambridge Manufacturing Director Notices 1 About the system Use only with Thermo Electron approved computer and accessories 2 About Shielded Cables Use only shielded cables supplied by Thermo Electron when connecting this instrument to computer and accessories Compliance with the abov
78. ty at 546nm using neutral density glass filters traceable to the NPL European Pharmacopoeia photometric accuracy test using potassium dichromate 36 Issue 1 August 2003 Peak Peak Noise 500nm OA 1 5nm 1A 1 5nm 2A 1 5nm Stray Light KCI solution at 200 nm Nal solution at 220 nm NaNO solution at 340 nm Stability at 340nm Baseline Flatness Scan Speeds Physical Specifications Dimensions Height Width Depth Weight Electrical Specifications Voltage Frequency Power Environmental Specifications Temperature Range Humidity Range Safety Electromagnetic Compatibility 8 3 Regulatory Notices Electrical Safety Class Nicolet Evolution 500 User Manual 0 0003A 0 0004A 0 0040A gt 2 0A lt 0 02 T lt 0 005 T lt 0 0005 A Hour 0 001A 1 3800nm min Intelliscan 248mm 552mm 584mm 29kg 100 120 220 240V ac 10 50 60Hz 350VA max 5 40 C 20 80 non condensing IEC1010 1 EN50082 1 This apparatus has been designed and tested in accordance with Safety Class requirements of IEC Publication No 1010 1 safety requirements for electrical equipment for measurement control and laboratory use and has been supplied in a safe condition The present instruction manual contains some information and warnings which have to Baseline flatness 200 to 800nm 120nm min 2nm data interval medium smoothing 37 Issue 1 August 2003 Nicolet Evolution 500 User Manual
79. ust Electronic equipment should not be used in dusty areas 10 Issue 1 August 2003 4 2 Nicolet Evolution 500 User Manual Connecting the spectrophotometer to the Mains 4 2 4 Earthing Grounding Before any other connection is made the spectrophotometer must be connected to a protective earth conductor via the three core mains cable see Mains Cable Connection Section 4 2 3 The mains plug must only be inserted into a socket outlet provided with a protective earth contact The protective action must not be negated by the use of an extension cable without a protective conductor WARNING Any interruption of the protective earth conductor inside or outside the instrument is likely to make the instrument dangerous No responsibility will be accepted for any consequences of failure to ensure adequate earthing grounding 4 2 2 Mains Voltage Setting and Mains Fuse The Nicolet Evolution 500 can be operated on mains voltages of nominally 10 100V 120V 220V and 240V 50 and 60 Hz Before connecting the mains cable and switching on check and reset the selector as follows WARNING The instrument must be disconnected from all voltage sources before being adapted to a different mains voltage or when a fuse isto be renewed 1 Look through the window into the selector Fig 4 1 and check that the voltage marked on the selector board and the fuse value see 4 2 2 3 are correct for your mains supply If these are OK move on to
80. ware of and apply the following safety precautions 3 4 Safety Precautions For the correct and safe use of this instrument and its accessories it is essential that both operating and servicing personnel follow generally accepted safety procedures in addition to the safety precautions specified in this manual Specific warning and caution statements where applicable can be found throughout this manual Warning and caution statements and or symbols are marked on the apparatus where necessary The instrument covers and accessories should be removed only by personnel who have been trained to avoid the risk of electric shocks The mains electricity supply to the instrument must be disconnected at the mains supply connector and at least three minutes allowed for capacitors to discharge Some of the chemicals used in spectrophotometry are corrosive and or flammable and samples may be radioactive toxic or potentially infectious Care should be taken to follow the normal laboratory procedures for handling chemicals The UV radiation from a Deuterium lamp can be harmful to the skin and eyes Always view the lamp through protective glasses goggles that will absorb the UV radiation and avoid looking directly at the Deuterium arc Do not expose the skin to direct or reflected UV radiation 3 2 Cautions and Warning Statements Caution Used to indicate correct operating or maintenance procedures in order to prevent damage to or destruction of equipme
81. warranties of merchantability and fitness for a particular purpose Copyright 2003 by Thermo Electron Corporation Madison WI 53711 Printed in the United States of America All world rights reserved No part of this publication may be stored in a retrieval system transmitted or reproduced in any way including but not limited to photocopy photograph magnetic or other record without the prior written permission of Thermo Electron Corporation For technical assistance please contact Technical Support Thermo Electron Corporation 5225 Verona Road Madison WI 53711 4495 U S A Telephone 1 800 642 6538 U S A or 1 608 273 5015 worldwide Fax 1 608 273 5045 worldwide E mail techsupport analyze 0 thermo com ABBE 3L AIR Almega AMINCO Bowman Antaris Analyze Detect Measure Control ApPro AquaMate ARK Atlus ATMOS Aurora Avatar BACKCOMM Baseline BEAM BioMate Blocker Centaurus Chemigram CIRCLE Collector Contact Alert Contact Sampler Continuum Corner Cube CSA 4K DeterminatIR DSolve DX ECO Educator ENCORE Encompass Endurance E S P Ever Glo Evolution EZ Flow EZ Scope Far IR Solid Substrate FiberLink FiberPort FLEXSYS Flex Top Foundation Series Full Field Aperture Gemini Genesis II GENESYS G XT Helios ImageMax Impact Import Infinity Infinity Gold InspectIR Instruments of Discovery Integra Interlinking InterpretIR InterpretIR IR Plan IRus ISOBE Laser Quadrature Lo

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