JS-5.2 & JS-4.0 Swinging Bucket Rotors
Contents
1. Correct Incorrect 000088 00000000 OO OO OO0000000 O0000000 e 000088 eeeeeeee 00 OO OO0000000 e 000088 O0000000 OO OO OQOOOO00O 0000000 COOOO0OO 00 OO O0000000 0000000 OO0000000 OO OO 000088 O00000 OQ OO 000088 OQOOOO00O OO OO e 000088 OQOOOOO0OO OO OO 0000000 OOQ000000 00 OO 000088 OO0O000000 OO OO 000088 eeeeeee OO OO 000088 0000000 OO OO Examples of Symmetrically Loaded Trays Examples of Nonsymmetrically Load Opposite Trays the Same Way Loaded Trays O Pivotal Axis of Bucket Carrier QOOOOO0000000 OOCOOC000000 D O0O0000000000 OOO00Q00000000 OOOOO0000000 OOO OOOO 00 O OO0O0000000000 OO0000000000 OO0O0000000000 O Figure 5 Examples of Correctly and Incorrectly Loaded Buckets and Carriers Contents of opposing buckets or carriers must be the same and each bucket or carrier must be balanced on its pivotal axis JS 5 2 and JS 4 0 Rotors AT REST AT SPEED AT REST AT SPEED Ore Proper Weight Distribution Improper Weight Distribution Figure 6 Examples of the Effects on the Horizontal Swing of Buckets Caused by Proper and Improper Weight Distribution Assembling Multi Disc Adapters 1 Slide the U shaped bracket into the grooved black rubber adapter base see Figure 7 Modified bracket 343369 is required if you are using Aeroseal Covers 2 Place the base and bracket on a lab bench not in the rotor 3 Position one of the disks so2. 1 5 mL 356090 Rack insert white 373696 373661 polypropylene clear pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356091 Rack insert white 373696 373661 polypropylene blue pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356092 Rack insert white 373696 373661 polypropylene green pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356093 Rack insert white 373696 373661 polypropylene yellow pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356094 Rack insert white 373696 373661 polypropylene orange pkg 500 pkg 25 holds 24 tubes INSTALLING THE ROTOR JS 5 2 and JS 4 0 Rotors e If you are using a microprocessor controlled J6 series centrifuge enter rotor code 5 2 for the JS 5 2 or code 4 0 for the JS 4 0 rotor e Apply a thin film of Spinkote lubricant to the centrifuge drive spindle hub Two drive pins inside the rotor drive hole see Figure 1 engage with teeth on the centrifuge drive spindle hub to prevent the rotor from slipping during acceleration and deceleration When the rotor is properly installed the pins sit in the drive hub grooves or next to the drive hub teeth see Figure 2 Rotor Drive Hole Drive Hub Teeth Vv Drive Pins Figure 1 Rotor Drive Pin Location and Orientation Centrifuge Drive Hub 1 Hold the rotor with both hands and carefully lower the rotor yoke straight down onto the centrifuge drive spindle hub Slowly rotate
3. 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 44 01494 441181 Fax 44 01494 447558 e mail beckman_uk beckman com Additional addresses are available at www beckmancoulter com 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 Internet www beckmancoulter com Telex 678413 Fax 1 800 643 4366 2007 Beckman Coulter Inc G Bd Printed on recycled paper
4. adapter 356997 5 200 rpm 50 mL w snap on cap pkg 25 29 x 104mm polypropylene thick 357005 36 5 white 29 mm cap 356264 5 200 rpm 50 mL wall tube snap on pkg 25 red 29 mm cap 357359 cap green 29 mm cap 357360 yellow 29 mm cap 357361 blue 29 mm cap 357362 29 x 104mm polypropylene thick 357007 34 none 5 200 rpm 50 mL wall tube no cap pkg 25 18 x 98 mm polyethylene tube 3420814 15 adapter 339102 5 200 rpm 15 mL open top pkg 100 18 x 98 mm polycarbonate tube 3420804 15 adapter 339102 5 200 rpm 15 mL open top pkg 100 18 x 100 mm polypropylene tube 3420824 15 adapter 339102 5 200 rpm 15 mL open top pkg 100 17x119mm conical 355663 15 adapter 356995 5 200 rpm 15 mL polypropylene pkg 6 open top tube 16 x 80 mm polycarbonate bottle 355672 10 adapter 341977 5 200 rpm 10 mL wi cap pkg 25 16 x 76mm stainless steel tube 301108 10 adapter 341977 5 200 rpm 10 mL open top pkg 1 16 x 76 mm polycarbonate tube 355630 10 adapter 341977 5 200 rpm 10 mL open top pkg 25 16 x 76 mm polyallomer tube 355640 10 adapter 341977 5 200 rpm 10 mL open top pkg 25 Continued a Above 20 C fill polypropylene tubes at least half full b Maximum speeds listed are for the JS 5 2 rotor and are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of manufacturing variances no guarantee of performance or fit is expressed or implied The maximum speed of all tubes and bottles in
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6. rotor components including those made of plastic and rubber See Appendix A in Rotors and Tubes for more information regarding chemical resistance of tubes bottles and accessories 121 C While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer Ganon ut 5 Flammability hazard Do not use in or near operating centrifuges 31 JS 5 2 and JS 4 0 Rotors 32 RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form which may be obtained from your local Beckman Coulter sales office should contain the following information e serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and e name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Ste
7. the yoke back and forth to make sure that it is properly seated A CAUTION Never drop the rotor yoke onto the drive spindle hub The drive spindle can be bent if the yoke is dropped onto it 15 JS 5 2 and JS 4 0 Rotors 16 Drive Rotor Hub Pins 2 Grooves 4 Drive Hub lt lt Drive Spindle Assembly Older Model Centrifuges Be sure the pins in the rotor drive hole are seated in the grooves of the drive spindle hub as shown ee Drive Hub Ps Drive Spindle Assembly Newer Model Centrifuges Be sure the pins in the rotor drive hole are not sitting on top of the teeth on the drive spindle hub Figure 2 Centrifuge Drive Spindle Hub Configuration 2 After the rotor is properly seated secure it to the drive hub with the tie down bolt 368518 Tighten the bolt with the tie down tool 368521 then remove the tool This system is shown in Figure 3 Older rotors may have a tie down screw 339031 which is tightened with a torque bar 878439 as shown in Figure 4 Rotor Tie Down Bolt 368518 Tie Down Tool 368521 Figure 3 Using the Tie Down Bolt and Tie Down Tool to Tighten the Rotor onto the Drive Hub JS 5 2 and JS 4 0 Rotors HY rte US Rotor p Tie Down a Bae Screw 339031 DN Torque Bar ra al 878439 Figure 4 Using the Tie Down Screw and Torque Bar to Tighten the Roto
8. 0 267 00 85 13 Fax 420 267 00 83 23 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 365 3707 Fax 41 22 365 0700 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 33 1 49 90 90 00 Fax 33 1 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 51 33 35 Fax 49 21 51 33 33 e mail bioresearch de beckman com GS zraou COULTER Beckman Coulter Inc e Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 Fax 852 2873 4511 INDIA Beckman Coulter India Pvt Ltd Solitaire Corporate Park 3rd Floor Bldg 11 Andheri Ghatkopar Link Road Chakala Andheri West Mumbai India 400 098 Telephone 91 22 3080 5101 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 39 02 953921 Fax 39 02 95392264 JAPAN Beckman Coulter K K TOC Ariake West Tower 2 5 7 Ariake Koto ku Tokyo 135 0063 Japan Telephone 81 3 5530 8500 Fax 81 3 5404 8436 MEXICO Beckman Coulter de Mexico S A
9. 2 min Weight of fully loaded rotor 17 kg 37 1b Rotor and bucket material anodized aluminum Conditions requiring speed reduction see RUN SPEEDS Rotor entry code for microprocessor controlled INSHUMENS iis eign ee oa ea ee hed D E ee se eae 4 0 _ Tay gt max Axis of Rotation The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 1 amp where r is the radius in millimeters is the angular velocity in radians per second 2 RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution U S Pat No 4 009 824 RPM 2 RCF 1 12r E 1000 JS 5 2 and JS 4 0 Rotors DESCRIPTION These rotors have been manufactured in a registered ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges ROTORS The JS 5 2 and JS 4 0 swinging bucket rotors are rated for maximum speeds of 5200 and 4000 rpm respectively These rotors share a common four place rotor yoke the JS 5 2 has a windshield around the yoke and buckets and the JS 4 0 has n
10. 4 0 Rotors 10 Table 1 Available Tubes and Bottles for the JS 5 2 and JS 4 0 Swinging Bucket Rotors continued b Maximum speeds listed are for the JS 5 2 rotor and are guidelines only These speeds have been achieved in reliability tests Tube Required Accessory Max Dimensions Part Fill Vola Part and Volume Description Number mL Description Number Max Speed gt 62 x 120 mm polypropylene wide 356011 250 none 5 200 rpm 250 mL mouth bottle w cap pkg 6 assy 62 x120mm polycarbonate wide 356013 250 none 5 200 rpm 250 mL mouth bottle w cap pkg 6 assy 60 x 120 mm conical 356987 230 adapter 356983 5 200 rpm 230 mL polycarbonate bottle pkg 6 339108 w screw cap 60 x 120 mm conical polyallomer 356989 230 adapter 356983 5 200 rpm 230 mL bottle w screw cap pkg 6 38 x 102 mm polypropylene 355626 100 adapter 339104 5 200 rpm 100 mL bottle no cap pkg 6 38 x 102 mm polypropylene bottle 355624 100 adapter 339104 5 200 rpm 100 mL w cap pkg 6 38 x 102 mm polycarbonate bottle 355620 70 adapter 339104 5 200 rpm 70 mL w cap assy pkg 6 38 x 102 mm polycarbonate 355655 70 adapter 339104 5 200 rpm 70 mL bottle no cap pkg 6 29 x 104mm polyallomer bottle 361694 50 adapter 356997 5 200 rpm 50 mL assy w snap on cap pkg 24 29 x 104mm polycarbonate 363647 50 adapter 356997 5 200 rpm 50 mL open top tube pkg 25 29 x 104mm polycarbonate bottle 35
11. 7000 45 none 5 200 rpm 50 mL assy liquid tight cap pkg 6 assy 29 x 104mm polyallomer bottle 357001 45 none 5 200 rpm 50 mL assy liquid tight cap pkg 6 assy 29 x 104mm polycarbonate bottle 357002 40 none 5 200 rpm 50 mL w screw cap pkg 25 a Above 20 C fill polypropylene tubes at least half full Continued at Beckman Coulter but because of manufacturing variances no guarantee of performance or fit is expressed or implied The maximum speed of all tubes and bottles in the JS 4 0 rotor is 4000 rpm Package of 25 d To order caps for 15 mL tubes 342080 342081 and 342082 use part number 343656 for a package of 50 Caps 343656 are made of Hytrel thermoplastic polyester elastomer Hytrel is a registered trademark of E I Du Pont de Nemours amp Co Note that Hytrel does not provide the same chemical resistance as the tube materials Before using the caps check with the manufacturer to verify Hytrel s ability to withstand exposure to the chemicals you will be using JS 5 2 and JS 4 0 Rotors Table 1 Available Tubes and Bottles for the JS 5 2 and JS 4 0 Swinging Bucket Rotors continued Tube Required Accessory Max Dimensions Part Fill Vola Part and Volume Description Number mL Description Number Max Speed gt 29 x 104mm polyallomer bottle 357003 40 none 5 200 rpm 50 mL w screw cap pkg 25 29 x 104mm polycarbonate tube 363664 36 5
12. CF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 222 8 where r is the radius in millimeters is the angular velocity in radians per second 2 RPM 60 and g is the standard acceleration of gravity 9807 mm s2 After substitution RPM 2 RCF 1 12 RM e T000 JS 4 0 gt NOTE The JS 4 0 rotor can be used in Beckman Coulter Avanti J 26XP series discontinued Avanti J 20 series and J6 series centrifuges SPECIFICATIONS Maximum speed 0 0 0 c eee eee eee eee 4000 rpm Critical speed range 0 0000 000 600 to 800 rpm Maximum solution density 0 0000 1 2 g mL Maximum allowable imbalance of opposing loads 10 grams Relative Centrifugal Field at maximum speed At friax 226 MM pepee cd nd ep na Peden ede ee es 4050 x g Atra JSO MM 9 5 65 ees boo ob heer ee gees eee 2800 x g At rmin 86MM eperera keien ernia r eee 1540 x g Number of buckets 4 Nominal capacity per bucket 1 liter bottle or 1 quad pack blood bag Nominal capacity of rotor 4 liters 4 blood bags 12 microplates 148 RIA tubes Approximate acceleration time to maximum speed rotor fully loaded 0 0 eee eee eee 2 min Approximate deceleration time from maximum speed rotor fully loaded 0 eee ee eee ee 11
13. J6 Series Centrifuges Rotor Required Sample Temperature C green bar Speed rpm 2 C 4 C 8 C 10 C 15 C 20 C 30 C 5200 3 1 4 6 10 17 28 4000 1 1 5 7 12 19 29 3000 0 2 7 14 20 30 2000 and 2 4 8 10 15 20 30 below JS 5 2 and JS 4 0 Rotors b Place the filled precooled rotor in the centrifuge and run the system for at least one half hour c Measure the water temperature If it is lower than the required run temperature enter positive compensation units during run setup following steps 3 and 4 below If it is higher than the required run temperature enter negative compensation units during run setup also using steps 3 and 4 below d Discard the water Fill tubes with sample load tubes into the rotor and precool the system to the required temperature before starting the run 3 Press COMP ADJ The word COMP flashes below the TEMPERA TURE display and the display flashes 4 Use the keypad to enter the compensation value Press the key to enter a minus sign pressing it again will remove the minus sign 5 Check the temperature display If the entry is incorrect press CE and reenter the digits 6 When the entry is correct press ENTER RECALL I gt NOTE To clear a COMP ADJ entry press COMP ADJ 0 and ENTER RECALL CARE AND MAINTENANCE INSPECTION e Periodically at least monthly inspect the rotor yoke and buckets for rough spots or pit
14. J6 TB 006K EI December 2007 BECKMAN COULTER Instructions for Using JS 5 2 and JS 4 0 Swinging Bucket Rotors JS 5 2 Used in Beckman Coulter J6 Series Centrifuges JS 4 0 Used in Beckman Coulter Avanti J 26XP Series and J6 Series Centrifuges 2007 Beckman Coulter Inc Printed in the U S A JS 5 2 and JS 4 0 Rotors SAFETY NOTICE This safety notice summarizes information basic to the safe use of the equipment described in this publication The international symbol displayed above is a reminder to the user that all safety instructions should be read and under stood before operation or maintenance of this equipment is attempted When you see the symbol on other pages of this publication pay special attention to the specific safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor system This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval ance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for ae
15. S Maximum speed so sod eda eee ee dae be ee eens 5200 rpm Critical speed range 0 0 00 0 00 00 00008 600 to 800 rpm Maximum solution density 0 0000 1 2 g mL Maximum allowable imbalance of opposing loads 10 grams Relative Centrifugal Fieldt at maximum speed At finax 226 TM oces ee kee ek dee ee ee oe 6840 x g At ray 156 10M 2b ose eck neet bere a hare woe ast 4720 x g At Tmin 80 MM noice ae Lada hb ede Pa E 2600 x g Number of buckets 20 0 260 bide sb ade serrer eta niriy 4 Nominal capacity per bucket 0 000 1 liter bottle or quad pack blood bag Nominal capacity of rotor 4 liters 4 blood bags 12 microplates 148 RIA tubes Approximate acceleration time to maximum speed rotor fully loaded 1 ee eee eee 2 1 2 min Approximate deceleration time from maximum speed rotor fully loaded 0 0 eee eee eee 3 min Weight of fully loaded rotor 0 21 kg 46 lb Rotor and bucket material anodized aluminum Conditions requiring speed reduction see RUN SPEEDS Rotor entry code for microprocessor controlled IOSHCUMIENES cisco ds eigie tng enon t aie ena adie E id edie ate ea 5 2 The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration Relative Centrifugal Field R
16. a tional speed is selected so that identical samples are subjected to the same RCF in two different rotors the samples are subjected to the same force see Table 5 The data in Table 5 apply to the JS 4 0 rotor only up to its maximum rated speed of 4000 rpm gt NOTE Entries in Table 5 are calculated from the formula RCF 1 2 r RPM 1000 2 and then rounded to three significant digits 24 Table 5 Relative Centrifugal Fields Entries in this table are calculated from the formula RCF 1 12 r RPM 1000 and then rounded to three significant digits JS 5 2 and JS 4 0 Rotors Relative Centrifugal Field x g Buckets Microplates Rotor Speed At Imax At fay At fmin At Imax At fay ARR rpm 226 mm 156 mm 86 mm 192 mm 178 mm 164 mm 5200 6840 4720 2600 5000 6330 4370 2410 4500 5130 3540 1950 4000 4050 2800 1540 3500 3100 2140 1180 3000 2280 1570 866 2600 1710 1180 651 1459 1350 1240 2500 1580 1090 602 1340 1250 1150 2000 1010 698 385 860 797 734 1500 569 393 216 483 448 413 1000 253 174 96 215 199 183 The rotors can process solutions at the maximum rated speeds only if the solution density is 1 2 g mL or less Rotor speeds must be reduced from the rated maximum under any of the following circumstances e Ifthe JS 5 2 rotor is run without a lid limit the speed to 1450 rpm The additional windage created by not using the lid will overload the centrifuge drive and ref
17. arbonate bottle 355649 500 adapter 339109 5 200 rpm 500 mL no cap pkg 6 69 x 159mm polypropylene bottle 355665 500 adapter 339109 5 200 rpm 500 mL w screw cap pkg 6 69 x 159mm polypropylene bottle 355650 500 adapter 339109 5 200 rpm 500 mL no cap pkg 6 62 x 136 mm polycarbonate round 355673 250 adapter 339108 5 200 rpm 250 mL bottom bottle w screw pkg 6 cap 62 x 122 mm polycarbonate wide 358275 250 adapter 339108 5 200 rpm 250 mL mouth bottle pkg 6 62 x 122 mm polypropylene wide 358326 250 adapter 339108 5 200 rpm 250 mL mouth bottle pkg 6 Continued a Above 20 C fill polypropylene tubes at least half full b Maximum speeds listed are for the JS 5 2 rotor and are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of manufacturing variances no guarantee of performance or fit is expressed or implied The maximum speed of all tubes and bottles in the JS 4 0 rotor is 4000 rpm Package of 25 d To order caps for 15 mL tubes 342080 342081 and 342082 use part number 343656 for a package of 50 Caps 343656 are made of Hytrel thermoplastic polyester elastomer Hytrel is a registered trademark of E I Du Pont de Nemours amp Co Note that Hytrel does not provide the same chemical resistance as the tube materials Before using the caps check with the manufacturer to verify Hytrel s ability to withstand exposure to the chemicals you will be using JS 5 2 and JS
18. cation See Table 2 for the appropriate adapter discs for use with different size tubes and bottles Blood Bag Cups Polypropylene cups provide support for blood bags in the rotor buckets A single or double pack blood bag cup and a triple or quad pack cup are available and are listed in Table 3 Aeroseal Covers for Buckets The Aeroseal covers 343686 are designed to minimize leakage of aerosol particles from buckets during centrifugation Aeroseal covers can be used with Multi Disc adapters with modified bracket 343369 but not with blood bag cups For more information refer to publication J6 TB 017 which is shipped with the covers JS 5 2 and JS 4 0 Rotors TUBES AND BOTTLES The JS 5 2 and JS 4 0 rotors use the tubes and bottles listed in Table 1 Be sure to use only those items listed and to observe the maximum speed limits and fill volumes shown Refer to Appendix A in Rotors and Tubes for chemical compatibilities of tube bottle and accessory materials Temperature Limits e Plastic containers have been centrifuge tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated conditions If plastic containers are frozen before use make sure that they are thawed to at least 2 C before centrifugation Gutta J Thickwall Tubes S Thickwall polyallomer polypropylene and polycarbonate tubes can be run partially filled with or without caps b
19. conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions of sale in effect at the time of sale Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a J series rotor to the original buyer by Beckman Coulter Inc or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this war ranty Beckman Coulter will supply free of charge all centri fuge parts required for repair REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the trans portation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all trans portation charges A defective rotor will be replaced by Beckman Coulter at its then current list price less a credit based upon the age of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Palo Alto California facility or delivered to a Beckman Coulter Field Service representative The replaceme
20. e centrifuge e If two blood bags are run place them in cups in opposing buckets Place empty buckets in the other positions Do not run the rotor with fewer than four buckets installed whether loaded or empty 23 JS 5 2 and JS 4 0 Rotors LOADING MICROPLATE CARRIERS 1 To prevent multiwell plate breakage during centrifugation place a rubber pad 341830 on the bottom of each carrier 2 Tilt the carrier and insert the filled labware being careful not to spill the contents A 96 well cap strip 267002 may be placed over a filled multiwell plate to prevent leakage If you are using two or three multiwell plates per carrier place a cap strip 267002 between the plates to prevent breakage during centrifugation gt NOTE Do not intermix microplate carriers for the Beckman Coulter JS 5 2 and JS 4 0 rotors with those used in the JS 4 2 and JS 3 0 rotors The carriers look similar but have different weights and maximum speeds Each carrier is marked with its maximum speed and the rotors it can be used in before each run check that you are using the correct carriers for your rotor Mixing carriers during a run can cause imbalance See publication J6 TB 009 shipped with the carriers for more information RUN SPEEDS The centrifugal force at a given radius in a rotor is a function of the rotor speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rot
21. erve proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection When working with potentially hazardous materials always fill or open containers in an appropriate hood or biological safety cabinet Capped tubes or bottles are designed to provide fluid containment We strongly recommend that all containers carrying physiological fluids be capped to prevent leakage A CAUTION Load adapters cups buckets or microplate carriers first before placing them into the installed rotor to avoid damaging the centrifuge LOADING BUCKETS JS 5 2 and JS 4 0 Rotors Symmetric and Balanced Loading To ensure optimal performance and stability load the rotors symmet rically see Figure 5 Two factors affect symmetric loading e the rotor must be loaded symmetrically with respect to its center of rotation e the buckets or microplate carriers must be loaded symmetrically with respect to their pivotal axes This means that for best results you should load opposing buckets or carriers with the same type of labware containing the same amount of fluid of equal density Two or four sample loads can be run Opposing buck
22. ets or carriers and their contents must weigh within 10 grams of each other During a run buckets or carriers swing 90 degrees from their at rest position The pivotal axis of a bucket can be imagined as a line extending across the bucket from one pivot pin to the other If a bucket is loaded so that its weight is unequally distributed on either side of its pivotal axis it will not hang vertically at rest and more importantly may not swing to a horizontal position during a run see Figure 6 As a result extra stress will be placed on the bucket and labware during the run increasing the possibility of breakage or rotor imbalance Load buckets before or after they are installed on the rotor yoke In either case we recommend filling the labware first and then loading the labware into the buckets This is especially important when using blood bags you can trip the imbalance detector in the centrifuge by pushing blood bag cups into buckets installed in the rotor Refer to Tables 1 through 4 to determine the appropriate labware for your application Whether you are running tubes bottles or blood bags you must load the buckets symmetrically around the center of rotation and each bucket must be loaded symmetrically with respect to its pivotal axis see Symmetric and Balanced Loading above 19 JS 5 2 and JS 4 0 Rotors 20 Balanced Load Unbalanced Load Pivotal Axis of Bucket Center of Rotation
23. f tubes microplates or other labware made by manufacturers other than Beckman Coulter are used reduce rotor speed to prevent breakage The strength of glass and plastic tubes can vary between lots and will depend on handling and usage we highly recommend that you pretest labware in the rotor using water samples to determine optimal operating conditions Scratches even microscopic ones significantly weaken glass tubes gt All four positions on the rotor yoke must contain either a bucket or a carrier loaded or unloaded during arun Never run the rotor without all of the positions being filled If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use lee i JS 5 2 lt _ max Axis of Rotation U S Pat No 4 009 824 U S Pat No 4 010 890 Canadian Pat No 1 063 989 French Pat No 77 00732 Japanese Util Mod No U M 1 462 551 gt NOTE The JS 5 2 rotor is used in Beckman Coulter J6 series centrifuges only It cannot be used in Avanti J series centrifuges SPECIFICATION
24. he marked expiration date is less than 7 years from the date of purchase the expiration date becomes the date of purchase plus seven 7 years Use of a bucket after such expiration date voids Beckman Coulter s warranty obligations with respect to any rotor and or centrifuge in which such a bucket is used DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 1 905 819 1234 Fax 1 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 CZECH REPUBLIC Beckman Coulter Prague Radiova 1 102 27 Prague 10 Czech Republice Telephone 42
25. n place of the buckets normally used with the rotor The carriers are made of aluminum and are black anodized for corrosion resis tance Each microplate carrier can accommodate up to three 96 well multiwell plates when more than one plate is run up to three plates are placed directly on top of one another 13 JS 5 2 and JS 4 0 Rotors 14 Refer to Table 4 for a list of labware that can be used with the microplate carriers Rotor speed must not exceed 2600 rpm when microplate carriers are run For complete microplate carrier informa tion see publication J6 TB 009 included with the carriers OPERATION e Precool the rotor and buckets or carriers in the centrifuge or in a refrigerator before use especially before short runs to ensure that the rotor reaches the set temperature A suggested precooling cycle is a minimum of 30 minutes at 2000 rpm at the required temperature e If fluid containment is required use capped tubes or bottles It is strongly recommended that all containers carrying physiological fluids be capped to prevent leakage Table 4 Labware for Use with Microplate Carriers Required Accessory Part Description Volume Number Description Part Number Rack Multiwell plate 300 pL well 609844 96 well nonsterile pkg 100 without caps Microfuge tube 1 5 mL 357448 Rack insert white 373696 373661 polyallomer clear pkg 500 pkg 25 holds 24 tubes Microfuge tube
26. no guarantee of performance or fit is expressed or implied The maximum speed of all tubes and bottles in the JS 4 0 rotor is 4000 rpm c Package of 25 4 To order caps for 15 mL tubes 342080 342081 and 342082 use part number 343656 for a package of 50 Caps 343656 are made of Hytrel thermoplastic polyester elastomer Hytrel is a registered trademark of E I Du Pont de Nemours amp Co Note that Hytrel does not provide the same chemical resistance as the tube materials Before using the caps check with the manufacturer to verify Hytrel s ability to withstand exposure to the chemicals you will be using Table 2 Multi Disc Adapters Used with Tubes and Bottles Typical Max Tube Dimensions Number of Number Adapter Tube Bottle Tubes per of Tubes Part Sizes Used Diameter Length Adapter per Rotor Number Color 3 and 5 mL 12 mm 133 mm 37 148 339100 blue 10 mL 14 mm 133 mm 24 96 339101 orange 12 mL 16 mm 133 mm 19 76 341977 purple 20 mL 18 mm 133 mm 14 56 339102 gt green 50 mL 28 mm 136 mm 7 28 339103 yellow 50 mL conical 30 mm 136 mm 4 16 345386 It green 50 mL 35 mm 136 mm 4 16 341794 dark blue 100 mL 44 mm 165 mm 2 8 339104 brown 230 mL conical 62 mm 141 mm 1 4 339108 red 250 mL 62 mm 160 mm 1 4 339108 red 500 mL 70 mm 160 mm 1 4 339109 yellow Double Stacking Adaptere 3 and 5 mL 12 mm 75mm 19 76 339119 blue white a Additional discs can be added b This ada
27. nt price cost to Buyer for the respective rotor shall be calculated as follows Replacement price Current rotor list price x years 7 CONDITIONS 1 Except as otherwise specifically provided herein this war ranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance con trary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor charac teristics or is operated in a Beckman Coulter centrifuge that has been improperly disassembled repaired or modified 4 Each bucket whether purchased with a rotor assembly or purchased separately is covered by this warranty for seven 7 years from the date of purchase and will be replaced or repaired during such period according to the terms and conditions of this warranty The date of manufacture marked on the bucket may be earlier than the date of pur chase and the expiration date marked on the bucket which is seven 7 years after the date of purchase may be correspondingly offset 5 Buckets should not be used after the expiration date marked on the bucket If at the time of purchase t
28. ntains one rotor body with attached windshield one rotor cover assembly 339085 round buckets set of 4 341710 rotor tie down kit 367045 Rotor assembly JS 4 0 0 0 ee eee 339086 Contains one rotor body no windshield round buckets set of 4 341710 rotor tie down kit 367045 Rotor buckets set of 4 0 0 0 0 cc cece teens 341710 Aeroseal cover for round buckets 1 0 0000 ee eee 343686 Rotor cover assy JS 5 2 only 0 0 0 0 eee eee eee 339085 Blood bag Cups soi feed 4h hardened des eteted see Table 3 Microplate carriers set of 2 includes rubber pads 358680 Microplate carrier labware 0 0 eee eee eee see Table 4 Rubber pad for use in microplate carriers 1 341830 96 well cap strip nonsterile pkg 12 00 0 267002 Multi disc adapters polypropylene See Table 2 Tubes and bottles 0 eee eee see Table 1 Rotor Cleaning Kat c c csesee pees spe ah ee swans BEE 339558 Rotor tie down kit 2 ee eee 367045 Contains tie down bolt 368518 and tie down tool 368521 33 JS 5 2 and JS 4 0 Rotors 34 Silicone vacuum grease 1 OZ 0 eee eee ee eee 335148 Solution 555 Pat eiie Seebeck wwreyese Abeba ere en eed in 339555 Spinkote lubricant 2 oZ 0 0 eee eee eens 306812 Tri Flow Superior Lubricant with Teflon 2 0z 883371 J SERIES SWINGING BUCKET ROTOR WARRANTY Subject to the
29. o windshield Each rotor holds four buckets or four microplate carriers that hook over stainless steel pins set in the yoke and swing out to horizontal position during centrifugation The buckets hold a wide variety of sample containers including tubes bottles and blood bag cups The microplate carriers are used to perform serial dilution of small liquid volumes Rotor assemblies buckets and lids are made of anodized aluminum The rotors are each warranted for 7 years see the Warranty at the back of this manual PREPARATION AND USE Specific information about the JS 5 2 and JS 4 0 rotors is given here Use the J Series Rotors and Tubes Manual publication JR IM along with this rotor manual for complete rotor and accessory information A CAUTION Although rotor components and accessories made by other manufacturers may fit in the JS 5 2 and JS 4 0 rotors their safety in these rotors cannot be ascertained by Beckman Coulter Use of other manufacturers compo nents or accessories in these rotors may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components listed in this publication should be used in these rotors JS 5 2 and JS 4 0 Rotors PRERUN SAFETY CHECKS aN ROTOR PREPARATION BUCKETS AND ACCESSORIES Read the Safety Notice page at the front of this manual before using the rotor 1 Make sure that the rotor and lid if applicable are clean and show no signs of corr
30. olution that will not damage their anodized surfaces Beckman Coulter has tested a number of solutions and found several that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking 3 and Radiacwash 4 gt NOTE IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from surfaces While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of decontamination is expressed or implied Consult your laboratory safety officer regarding the proper decontamination methods to use If the rotor or other components are contaminated with toxic or patho genic materials follow appropriate decontamination procedures as outlined by your laboratory safety officer 3 In the United States contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 4 In the United States contact Biodex Medical Systems Shirley NY internationally contact the U S office to find the dealer nearest you 30 JS 5 2 and JS 4 0 Rotors STERILIZATION AND DISINFECTION The rotor yoke buckets microplate carriers and tube adapters can be autoclaved at 121 C for up to an hour Blood bag cups can be auto claved at that temperature for about 15 minutes Ethanol 70 5 may be used on all
31. or yoke and tie down bolt The Rotor Cleaning Kit 339558 contains two quarts of Solution 555 and brushes that will not scratch the rotor Thoroughly rinse the cleaned rotor and components with water and air dry Do not use acetone to dry the rotor When the rotor is dry lubricate the bucket sockets and pins as described under MAINTE NANCE Before reinstalling the rotor lightly lubricate the centrifuge drive hub with Spinkote to prevent the rotor from sticking Buckets 1 Clean the buckets with a mild detergent such as Solution 555 339555 diluted 10 to 1 with water then rinse thoroughly and dry completely 2 After cleaning lubricate bucket pin sockets with Tri Flow 883371 as described under MAINTENANCE 29 JS 5 2 and JS 4 0 Rotors DECONTAMINATION Multi Disc Adapters 1 To disassemble adapters for washing pull the bracket out of the groove in the disks then remove the disks and unsnap the bracket from the rubber bottom 2 Use a mild detergent such as Solution 555 339555 diluted 10 to 1 with water and a soft brush to scrub the adapters 3 Rinse and dry then reassemble 4 If necessary apply a light film of silicone vacuum grease 335148 to the grooves on the rubber adapter bottom to make reassembling easier Keep matched sets of adapters together do not intermix sets If the rotor yoke buckets and or microplate carriers become contam inated with radioactive material decontaminate them using a s
32. osion or cracking Check the chemical compatibilities of all materials used refer to Appendix A in Rotors and Tubes Verify that the tubes bottles and other labware being used are listed in Tables 1 through 4 For runs at other than room temperature refrigerate or warm the rotor before the run for fast temperature equilibration Before installing the rotor lightly coat the centrifuge drive hub with Spinkote lubricant 306812 Load the filled containers symmetrically into the rotor If you are running two containers place containers opposite each other on the yoke and place empty buckets in the other positions Opposing containers must be filled to the same level with liquid of the same density The round buckets used in the JS 5 2 and JS 4 0 rotors can hold bottle adapters Multi Disc adapters to accommodate tubes of various sizes and blood bag cups 1 U S Pat No 4 342 419 JS 5 2 and JS 4 0 Rotors Bottle Sleeve The one liter bottles used in the JS 5 2 and JS 4 0 rotors are supported in a polypropylene sleeve 356096 that fits inside the buckets Bottles available for use are listed in Table 1 Multi Disc Adapters The Multi Disc adapters are made up of polypropylene discs which are stacked and snapped together to accommodate a particular size bottle or tube The number of adapters required depends on the length of the tube or bottle The discs are color coded for identifi
33. pter also holds 15 mL conical tubes c To run 148 RIA tubes use four of the 37 hole adapters 339100 plus four of the 19 hole adapters 339119 Tube retaining device 343108 is required use of this device is described in publication IN 174 which ships with 343108 MICROPLATE CARRIERS JS 5 2 and JS 4 0 Rotors Table 3 Blood Bag Cups Number of Part Blood Packs Cups Number Description per Cup per Rotor set of 2 Blood bag cups yellow 1 single or 4 339127 88 mm inside diameter double pack Blood bag cups red 1 triple or 4 339129 98 mm inside diameter quad pack Stainless Steel Tubes Stainless steel tubes offer excellent resistance to organic solvents and heat but should not be used with most acids or bases They offer only marginal resistance to most gradient forming materials other than sucrose and glycerol Stainless steel tubes are very strong and can be centrifuged when filled to any level Stainless steel tubes can be used indefinitely if they are undamaged and not allowed to corrode They may be autoclaved as long as they are thoroughly dried before storage Microfuge Tubes Microfuge tubes 1 5 mL tubes with attached caps are made of clear polyallomer or of clear or colored polypropylene They are used in microplate carriers The number and arrangement of microfuge tubes in opposing carriers should be balanced Microplate carriers 358680 qty 2 are installed on the rotor pivot pins i
34. r onto the Drive Hub Older Rotors 3 JS 5 2 rotor only Place the lid onto the rotor carefully seating it on the windshield Make sure that the tie down bolt pin protrudes into the hole in the lid knob If you keep the rotor in the centrifuge re tighten the tie down bolt before each run INSTALLING THE BUCKETS OR MICROPLATE CARRIERS 1 Lubricate the contact area between the buckets or carriers and the pivot pins on the rotor yoke as described under MAINTENANCE 2 Place buckets or carriers over the pivot pins on the rotor yoke Fill all four positions with a bucket or carrier 3 Make sure that the buckets or carriers are properly seated by gently swinging them on the pivot pins A CAUTION All four positions on the rotor yoke must contain either a bucket or a microplate carrier loaded or unloaded during a run Never run the rotor without all four positions being filled 17 JS 5 2 and JS 4 0 Rotors 18 LOADING THE BUCKETS AND CARRIERS AN WARNING Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infec tious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols obs
35. rigeration systems above this speed e Maximum speed when microplate carriers are used is 2600 rpm in all rotors e When centrifuging solutions of densities greater than 1 2 g mL compute the maximum allowable speed from the following formula RPM maximum rated speed 2500 grams P where p the weight in grams of the bucket and its contents 25 JS 5 2 and JS 4 0 Rotors 26 RUN TEMPERATURE To ensure that the rotor reaches the required temperature during the run follow the appropriate instructions below for the centrifuge model being used Avanti J Series Centrifuges Enter the run temperature according to the instructions in your centri fuge instruction manual No additional input is required Microprocessor Controlled J6 Series Centrifuges Enter rotor code 5 2 for the JS 5 2 or rotor code 4 0 for the JS 4 0 and then follow the steps below 1 Press the TEMP key on the centrifuge control panel and then use the keypad to enter the sample temperature 2 Find the compensation value in Table 6 that corresponds with the set temperature and run speed The compensation values listed in Table 6 are approximates for all J6 series centrifuge models If precise temperature control is required determine the compensa tion settings empirically as follows a Fill the rotor with water filled tubes or bottles Precool the rotor and water to the required temperature Table 6 Temperature Compensation Settings for
36. riliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label provided on the RGA form to mail the rotor or parts to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office SUPPLY LIST JS 5 2 and JS 4 0 Rotors I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back of this manual or see the Beckman Coulter High Performance High Speed High Capacity Rotors Tubes amp Accessories catalog BR 8102 available at www beckmancoulter com for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS SUPPLIES Rotor assembly JS 5 2 2 0 eee 339087 Co
37. rosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection Handle body fluids with care because they can transmit disease No known test offers complete assur Dispose of all waste solutions according to appropriate environmental health and safety guidelines The rotors and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials such as chloroform or ethyl alcohol in or handle or store them near the centrifuge ae Components or accessories designed for other rotors may cause rotor mishap if used in these rotors Use only components and accessories that have been designed for use in these rotors The safety of rotor components and accessories made by other manufacturers cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the rotors may void the rotor warranty and should be prohibited by your laboratory safety officer I
38. t to horizontal posi tion and make sure that all tubes clear the yoke 2 Place filled tubes or bottles in the assembled adapters If all positions in an adapter are not filled load the adapter symmetri cally with respect to its pivotal axis see Symmetric and Balanced Loading above 3 Place the adapters into the buckets so that the brackets line up with the rotor pivot pins If two or four loaded adapters are run place them in opposite buckets and place empty buckets in the other positions on the yoke If three loaded adapters are run leave a space between each filled bucket on the rotor yoke and place three empty buckets in the open positions Loading Blood Bag Cups To load blood bag cups follow the recommended loading procedure provided by the blood bag manufacturer With any blood bag ensure the following conditions e When loaded into the cups and buckets the blood bags must be as vertical as possible with no folds at the top or corners e The superstructure of the blood bag protruding from the cup must not inhibit the bucket from swinging out to horizontal position Test each bucket by swinging it gently to make sure that it reaches horizontal position If it does not remove the cup from the rotor and reposition the blood bag so that it seats further into the cup Allowing a blood bag to contact the rotor yoke during centrifuga tion can cause the bucket to come off the pivot pins and can seriously damage the rotor and th
39. that its grooves are aligned with the bracket Push the disk down until the bracket snaps into the grooves Figure 7 Assembling a Multi Disc Adapter 21 JS 5 2 and JS 4 0 Rotors 22 4 Add more disks until the height of the assembly is nearly as tall as the tubes you will be using Remove or add disks to the bracket to accommodate shorter or longer tubes If the tubes fit too snugly in the adapter s rubber base apply a light film of powder such as talcum powder to prevent the tubes from sticking To disassemble the adapters pry back the bracket arms by hand and remove the discs As a safety precaution the discs have been designed so that they cannot slide up off the bracket I gt NOTE Do not intermix Beckman Coulter GH 3 8 rotor style Multi Disc adapters with adapters for the JS 5 2 and JS 4 0 rotors The adapters are similar in appearance see Figure 8 but they have very different weights Mixing them during a run can cause imbalance Brackets A To Brackets JS 5 2 GH 3 8 JS 4 3 Figure 8 Brackets Vertical Supports for the JS 5 2 Style Multi Disc Adapters and the GH 3 8 Style Modular Disk Adapters JS 5 2 and JS 4 0 Rotors Loading Adapters 1 First test to make sure that the tubes or bottles in the adapters will not contact the rotor yoke during centrifugation Place empty tubes or bottles in an adapter and then place the adapter in a bucket on the rotor yoke Manually swing the bucke
40. the JS 4 0 rotor is 4000 rpm Package of 25 4 To order caps for 15 mL tubes 342080 342081 and 342082 use part number 343656 for a package of 50 Caps 343656 are made of Hytrel thermoplastic polyester elastomer Hytrel is a registered trademark of E I Du Pont de Nemours amp Co Note that Hytrel does not provide the same chemical resistance as the tube materials Before using the caps check with the manufacturer to verify Hytrel s ability to withstand exposure to the chemicals you will be using JS 5 2 and JS 4 0 Rotors Table 1 Available Tubes and Bottles for the JS 5 2 and JS 4 0 Swinging Bucket Rotors continued Tube Required Accessory Max Dimensions Part Fill Vola Part and Volume Description Number mL Description Number Max Speed gt 14 x 55 mm polypropylene 566353 4 adapter 339101 5 200 rpm 4 mL Bio vial pkg 1000 11 x 39 mm polyallomer tube 357448 1 5 adapters 339100 5 200 rpm 1 5 mL w snap on cap pkg 500 354511 11 x 39 mm polypropylene tube 343169 1 5 adapters 339100 5 200 rpm 1 5 mL w snap on cap pkg 500 354511 11 x 39 mm polyethylene tube 340196 1 5 adapters 339100 5 200 rpm 1 5 mL w snap on cap pkg 500 354511 a Above 20 C fill polypropylene tubes at least half full b Maximum speeds listed are for the JS 5 2 rotor and are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of manufacturing variances
41. ting white powder deposits frequently aluminum oxide or heavy discoloration If any of these signs are evident do not run the rotor Contact your Beckman Coulter repre sentative for information about the Field Rotor Inspection Program and the rotor repair center e Regularly check the condition of the tie down bolt if it is worn or damaged replace it e Before each use inspect Aeroseal cover sealing surfaces especially the O ring groove It must be smooth and free of 27 JS 5 2 and JS 4 0 Rotors MAINTENANCE Lubrication Points all pins _ gt oe a F Lubrication Area N both pin sockets scratches Also ensure that the top 2 54 cm 1 in of the bucket is clean and smooth buckets with scratches or gouges in this surface will not seal properly Inspect the O ring and plug for nicks abrasions and other damage Replace damaged components with Beckman Coulter parts only do not use a substitute for the O ring it has been specifically selected for this application The rotor yoke buckets and microplate carriers are made of anod ized aluminum Do not use sharp tools on them as scratches in the anodized surface may lead to corrosion After cleaning and before reinstalling the rotor body in the centri fuge apply a light even film of Spinkote lubricant 306812 to the centrifuge drive hub to prevent the rotor from sticking Approximately once a week or e
42. ut all opposing tubes for arun must be filled to the same level with liquid of the same density J Do not overfill capless tubes Polycarbonate and Polyallomer Bottles Capped polycarbonate and polyallomer bottles may be run completely filled or partially filled All opposing containers for a run must be filled to the same level JS 5 2 and JS 4 0 Rotors Table 1 Available Tubes and Bottles for the JS 5 2 and JS 4 0 Swinging Bucket Rotors Use only the items listed here and observe fill volumes and maximum run speeds Tube Required Accessory Max Dimensions Part Fill Vola Part Max and Volume Description Number mL Description Number Speed gt 97 x 167 mm polycarbonate bottle 355675 1000 sleeve 356096 5 200 rpm 1000 mL w screw cap pkg 6 97 x 167 mm polypropylene bottle 355676 1000 sleeve 356096 5 200 rpm 1000 mL w screw cap pkg 6 96 x 130 mm polycarbonate bottle 358299 750 sleeve 356096 5 200 rpm 750 mL w screw cap pkg 6 96 x 130 mm polypropylene bottle 356855 750 sleeve 356096 5 200 rpm 750 mL w screw cap pkg 6 69 x 160 mm polycarbonate bottle 355664 500 adapter 339109 5 200 rpm 500 mL w screw cap pkg 6 69 x 160 mm polycarbonate wide 355605 500 adapter 339109 5 200 rpm 500 mL mouth bottle w cap pkg 6 assy 69 x 160 mm polypropylene wide 355607 500 adapter 339109 5 200 rpm 500 mL mouth bottle w cap pkg 6 assy 69 x 160 mm polyc
43. very 80 runs and after cleaning and or autoclaving lubricate the contact area between the buckets or carriers and the pins Saturate a lintless tissue with Tri Flow oil 883371 and wipe it on each bucket or carrier socket and pin Also put a drop of Tri Flow between the pin and the rotor yoke III NOTE Periodically remove Tri Flow that may accumulate by wiping the rotor windshield or centrifuge chamber clean with a lintless cloth or tissue Lubricate the O ring and plug of Aeroseal bucket covers with silicone vacuum grease 335148 Also lightly grease the inside top 1 2 cm 0 5 in of the bucket 2 Tri Flow is a registered trademark of Thompson amp Formby Inc 28 CLEANING Rotor Cleaning Kit 339558 JS 5 2 and JS 4 0 Rotors Under normal conditions remove the rotor from the centrifuge at least once a week for cleaning and lubrication Rotor Components e If spillage has occurred or if salt solutions or other corrosive materials are used clean the rotor and components immediately Do not allow corrosive materials to dry on rotor components e Approximately once a week or every 80 runs clean the pins and bucket pin sockets then lubricate as described under MAINTENANCE Most laboratory detergents are too harsh for use on aluminum rotors because they can damage the anodized surface Use a mild detergent such as Beckman Solution 555 339555 diluted 10 to 1 with water and a soft brush to clean the rot
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