H201-NIKON-TI-SR
Contents
1. H201 NIKON TI SR User Manual June 2015 H201 NIKON TI SR Compatible with the following XY stages Compatible with the following Okolab Controllers Nikon T Ti SR www oko lab com H201 T UNIT BL Page 1 H201 NIKON TI SR User Manual June 2015 Index Me Component and CNS SIONS EE 3 L Same RTE ga ere Pee ee ane EP ene E Pee tT eT EE 3 2 1 Available Sample e 3 SB MVAUADIC Lids sssssyiriseri rrira S seca sear eecanaarsccaeaas ess 4 Li SANS UC ME 5 Cy ae Ee Bo eee ere er Mere rare er en ORT Ore ve ee eee ee er ee err tren 6 I MVS gier E 6 sa Eiser INGCTIOC EE 7 4 Insertion of the Sample Feedback Temperature Sensor sessessessossossosoosoosoosoosoosoosoosoosoososeoseoseooo 8 5 Insertion of Sample Holder into Chamber sesesessesesesecesesesesescececececesesessesecececesesescecesesececeseseseeo 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic LOCKS ee EEN 9 7 Working with 1x3 and 1x2 chamber slides Magnetic lOCKS ccc ccc ccecccceccccecccceccceecccesccceccs 11 8 Connecting the Gas Supply and Filling the Humidity Module ee 12 9 WORKING with Eer 12 10 Connecting the Chamber with XY stage s sescesescecescecesoecesoececoecesoecesoecesoecesoeceseeceseeceseeceseeces 13 fe www oko lab com H201 NIKON TI SR User Manual June 2015 1 Components and dimensions H201 NIKON TI SR includes the following components Chamber main body Sli2. CTION LID 2 Glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample Compatible only with sample holder 1x35 M single H201 LASER INTERLOCK LID 2 Glass lid with safety switch Connects to laser controller and automatically turns laser off when lid is lifted Compatible only with sample holder 1x35 M single OPTIONAL not included with H201 NIKON TI SR www oko lab com Paget H201 NIKON TI SR User Manual June 2015 3 1 Sliding lid The Sliding Lid is a glass lid allowing for easy sample loading and pipetting The Sliding Lid is screwed onto the cham ber Figure 3 shows chamber dimensions with the Sliding Lid Figure 4 illustrates how to remove Sliding Lid when a differ ent Lid is necessary NOTE Sliding Lid MUST BE REMOVED when using any other Lid The Sliding Lid is fixed onto the chamber with 4 screws Screws location is indicated by letter A in Figure 4 image 2 and 3 Keep Sliding Lid fully closed to access screws labeled A in image 2 Open Sliding Lid to access screws labeled A in image 3 Sliding Lid e e 255 Distance between focal plane and upper side of the glass lid D Chamber top view 137 Figure 4 Assembly of the sliding lid www oko lab com pee H201 NIKON TI SR User Manual June 2015 3 2 Koehler Lid The Koehler Lid is a glass lid reducing the chamber s height to 22 mm and allowing imaging under Koehler illuminati
3. KON TI SR User Manual June 2015 8 Connecting the Gas Supply and Filling the Humidity Module Single silicon tubing carries output gas from the Okolab Gas Controller to the H201 NIKON TI SR Silicon tubing con nects to a gas input brass opening located on a side of the H201 NIKON TI SR See Figure 16 Connect the silicon tubing by gently pushing it onto brass opening H201 NIKON TI SR includes an integrated humidity module in order to maintain a high humidity percentage inside the chamber Fill the humidity module inside the chamber through the water input brass opening located on a side of the H201 NIKON TI SR See Figure 16 using a silicon tubing 2 mm ID and a syringe NOTE Do not exceed 0 09 Gas Input Water Input Figure 16 Connection with gas supply 9 Working with Perfusion H201 NIKON TI SR features 8 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x3 Remove small screws as needed before introducing perfusion tubing Figure 17 shows location of perfusion holes Perfusion dedicated opening 1 Frontal view 2 3D view Figure 17 Perfusion www oko lab com el 2 H201 NIKON TI SR User Manual June 2015 10 Connecting the Chamber with XY stage F
4. R INTERLOCK LID www oko lab com ew H201 NIKON TI SR User Manual June 2015 4 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201 NIKON TI SR see Figure 8 Frontal and 3D views Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 8 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 9 albus W E Insert the adapter by superimposing the red points Figure 9 Introduction of the Specimen Holder inside the Chamber Base www oko lab com pase H201 NIKON TI SR User Manual June 2015 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Magnetic Locks for 35 and 60 mm petri dishes Included in the corresponding sample holder Fig
5. ding glass lid for easy pipetting Integrated humidity module It is not necessary to select an additional humidity module Sliding Lid 25 Distance between focal plane we and upper side of the glass lid emm a Sample Holder Order Separately Chamber top view D Integrated Humidty Module Chamber Base Figure 1 H201 NIKON TI SR Components and Dimensions 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish www oko lab com be H201 NIKON TI SR User Manual June 2015 1x35 M single 1x60 M single 1xGS M single For 1 35mm Petri dish For 1 60mm Petri dish For 1 1 x3 chamber slide 1xLABTEK M single 1xLABTEK II M single For 1 Lab Tek 1 x2 chambered cover glass For 1 Lab Tek Il 1 x2 chambered cover glass Figure 2 Available sample holders 3 Available Lids The following glass Lids are available for H201 NIKON TI SR H201 SLIDING LID 2 For easy sample loading and pipetting Included in code H201 NIKON TI SR H201 KOEHLER LID 2 Reduces chamber height to 22 mm and allows imaging under Koehler illumination H201 INJE
6. ollow the steps shown in the images Figure 18 and listed below in order to correctly connect the chamber with XY stage 1 Place the chamber on the stage and tighten 4 captive screws See Figure 18 Image 1 and Image 2 Captive screws housings are indicated with letter B in Figure 18 Image 2 2 Use a 2 5mm metric Allen Wrench to tighten the four captive screws while keeping the chamber sliding lid open See Figure 18 Image 2 Captive Screws Figure 18 Connection of the Chamber with XY Stage www oko lab com bape 3
7. on The Koehler Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 5 shows chamber dimensions with Koehler lid H201 KOHLER LID 2 227 Distance between focal plane and upper side of the glass lid O o Chamber top view a 163 Figure 5 H201 NIKON TI SR H201 KOEHLER LID 2 3 3 Injection Lid The Injection Lid is a glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample NOTE Compatible with sample holder 1x35 M single ONLY The Injection Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 6 shows chamber dimensions with the Injection Lid www oko lab com s H201 NIKON TI SR User Manual June 2015 H201 INJECTION LID 2 22 Distance between focal plane and upper side of the glass lid O N Chamber top view kel Figure 6 H201 NIKON TI SR H201 INJECTION LID 3 4 Laser Interlock Lid The Laser Interlock Lid is a glass lid with a safety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 M single ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid H201 LASER INTERLOCK LID 2 Chamber top view de 163 Figure 7 H201 NIKON TI SR H201 LASE
8. ure 10 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings with variable diameter are also included in according to the needs of the user Figure 11 shows how to position the ring inside the sample holder Ring selection guide diagrams rings for 35 and 60 mm dish Figure 12 and Figure 13 respectively Figure 11 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com sec H201 NIKON TI SR User Manual June 2015 Grainer Petri35x10 Ibidi p Dish35mm low BD Falcon 35x10 Willco 35x10 Corning 35x10mm MatTek P35G X 14 X Figure 12 Ring selection scheme for 35 mm dish BD Falcon 60x15 Grainer Petri 60x15 Corning 60x15mm Willco60x15 Figure 13 Ring selection scheme for 60 mm dish www oko lab com page LO H201 NIKON TI SR User Manual June 2015 7 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 14 Magnetic lock for 1 x 3 chamber slide Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 www oko lab com bape 1 H201 NI
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H201 NIKON TI SR