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1.     Instruction Manual   R65       HOUR  The HOUR option gives the opportunity to show the time on the display when the heat is off     Follow these steps to navigate in the HOUR option     Press and hold UP DOWN key for 3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option  HOUR   appears in the display     Press and hold setpoint key to change  values     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  select between  ON  and  OFF      When the required setting is activated  let  go of the setpoint key     Press and hold UP DOWN key for 3  seconds to exit the menu        If you want to see the time on the display when the heat is turned on   Follow these steps to switch between time and temperature    While holding the setpoint key in  press   the HEAT key one time  then let go of the      setpoint key     5 BJD  The display now shows the time           Page 18 Kivex Biotec Ltd    Instruction Manual   R65       To change back to temperature    While holding the setpoint key in  press   the HEAT key one time  then let go of the      setpoint key  J B    The display now shows the temperature     Note The time is shown as a 24 hour clock e g 19 30 not 7 30 PM           REST    The REST function will restore all factory set values   Any changes made to the displayed temperature  unit readings and controller value will be reset     Note Calibration value in the TUNE option will remain
2.    K SYSTEMS    Instruction Manual    Trolley with warmed surface  R65    na  h  o  w      o  m  x  ul  2  x       TABLE OF CONTENTS Instruction Manual   R65       1  General information and service      0    20    00  eee 1  2  Unpacking and inspection        naaa se4see ne dv dado kn d YS 2  3  Accessories   6 vce d err EUREN UE YE RODEO Ree ee does 2  3 1 Standard accessories           0    eee ee eee 2  3 2 Optional equipment           0  0 0    ee ee ee es 2  4 SER IV at fs gerre 9 o35 unk CBee eR SERED be BES RS 4  4 1 Definition Of USB  6    aua 54 446 eed GT dox R3 EX SERRE E OER ERS 4  4 2 General description            0 0    eee ee ee eee 4  5  Features and operation   x22 3 oes Y UR CURRY Rx 5  5 1 Assembling the R65   4 csc ee ee ee eb ee t eae 5  5 2 Connection tothe mains            lee 5  5 3 Start of apparatus    1    ee ee es 5  5 4 Keyboard functions             0 00  eee ee ee eee 5  5 5 Operating the heated area       0 0 2    llle 6  5 6 Temperature setting andcontrol                0000s 6  5 7 Combination keys             0  ce eee 7  5 8 Temperature alarm    0 0 0 0    ee eee 7  5 9 Warming   D  lt scces    e 244456 28S ECE OS Oe EG EU PRO Rx 7  5 9 1 Safety Danan  2 rv uui CORE E 4s os Eee eRe EUR ERS 8  6  Menu function    aceto   aoneeuieyeeecea ta EXER spb SUR Sri e 9  6 1 Overview of options    2 0 0 0    llle 10  UPC Paes ar te ee a S Oe Ge 11  5232    5 3 895 Y ee ee a ee UR eee URUE 12  TAAT Bea owe do veges 44 88 SPEER eee eee ee kee ee 13  I
3.  3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option  St St   appears in the display     Press and hold setpoint key to change  values     While holding the setpoint key in  press the  arrow UP to change the minutes and arrow  DOWN key to change the hour     When the time is set  let go of the setpoint  key    Press and hold UP DOWN key for 3  seconds to exit the menu        Note If the function Heat is set  a dot will light up in the right side of display        St St is connected with HEAT  It must be chosen when the timer starts   To turn the heater on  please see the option HEAT     WARNING Always make sure when the timer function is being used  that the heated area is clear  of any objects that might be damaged by the heat or adversely affected by it in any  Way   Caution should always be exercised when a heated area is turned on without any  supervision           Page 15 Kivex Biotec Ltd    Instruction Manual   R65       HEAT    The HEAT option is used for the heater to turn on at a certain time     Note This function only works in conjunction with St St   If St St is set to 8 00 and heat function is on  the heater will automatically turn on at 8 00  using 24 hour clock        Follow these steps to navigate in the HEAT option     Press and hold UP DOWN key for 3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option  HEAT   appears in the display     Pr
4.  V  nge 3 5  3460 Birker  d  Denmark    Tel    45 45 99 56 00  Fax   45 45 99 56 19    E mail  info k systems dk  Internet  www k systems dk          Issue 1  Change note No  277    Service adress   Please refer to your local K SYSTEMS Kivex Biotec distributor for details of your nearest  authorised service agent                 CAUTION If the equipment is used in a manner not specified by this manual  the safety of the  user may be at risk and the equipment may be damaged  Always use the equipment as  outlined in this instruction manual           Page 1 Kivex Biotec Ltd    Instruction Manual   R65       2  Unpacking and inspection       e Unpack the equipment      Check the packing list to insure all accessories are there      Examine the packing material carefully for separately packed items     Checkthe trolley for external surface damage     e Check that the display is not broken or damaged     3  Accessories       3 1 Standard accessories  PSU External power supply    Order code  57053    Mains Cable    Standard Schuko   Order code  52768  UK   Order code  53886  US   Order code  52773       3 2 Optional equipment  Optional equipment consists of a range of warming blocks  Please contact your local distributor or  K SYSTEMS Kivex Biotec for details about the full range of warming blocks     Examples of different types of warming blocks    Warming block B01  Holds seven tubes of 017 mm    Falcon 352001  Order code  26001       Page 2 Kivex Biotec Ltd    Warming block B06  
5.  and not be reset        Follow these steps to navigate in the REST option     Press and hold UP DOWN key for 3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option  REST   appears in the display     Press and hold setpoint key to change  values     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key     When the display shows          let go of the  setpoint key    Press and hold UP DOWN key for 3  seconds to exit the menu         This means all factory values have now been restored        Page 19 Kivex Biotec Ltd    Instruction Manual   R65       7  User maintenance       7 1 Cleaning    The R65 is a low maintenance unit designed to be both easy to use and clean  plus it is durable due to its  construction     All surfaces should be cleaned with an 70  alcohol solution on a clean cloth or lint free paper towel   e Clean all surface components by wiping gently with gauze       To remove fingerprints or oil smudges  wipe with gauze slightly moistened with a mixture  of ether  70   and alcohol  30       Note Since solvents such as ether and alcohol are highly flammable  they must be handled  carefully  Be sure to keep these chemicals away from open flames or potential sources  of ignition     electrical equipment must be switched off    Also remember to always use these chemicals only in a well ventilated room           If smudges are difficult to clean  wipe them with a soft cloth slightly 
6. Holds twelve tubes of  12 mm    Falcon 352003  NUNC 343923    Order code  26006    Warming block B09  Holds two tubes of 017 mm    Falcon 352095  Order code  26018    Warming block B28  Holds one 4 well dish    NUNC 176740  Order code  26029    Warming block B30  Holds five flushing syringes  of 20 ml  22 5 mm    Order code  26013    Page 3    1       Instruction Manual   R65       Kivex Biotec Ltd    Instruction Manual   R65       4  User Manual       4 1 Definition of use    The K SYSTEMS Kivex Biotecs R65 Trolley is designed for warming the media used  during oocyte retrieval in both human and animal assisted reproduction  It can also be used for  maintaining a stable temperature of cells and specimens in a test tube syringe or similar     4 2 General description    The aluminium surface maintains a preset temperature in the range ambient to 49 9   C   The temperature is electronically controlled by a PID controller and can be read on the display  in increments of 0 1  C     RS 232    PSU connector    socket    Heated  surface                Page 4 Kivex Biotec Ltd    Instruction Manual   R65       5  Features and operation       5 1 Assembling the R65    No assembly needed  Just follow directions in 5 2 and the unit is now ready for use     5 2 Connection to the mains    The mains voltage is automatically regulated to the correct level by the main adapter  PSU    Connect the R65 to the mains supply cable lead   For safety reasons the unit has an external PSU and operates 
7. N key and Heat ON OFF  The heat in the  drawer is turned  back on        5 8 Temperature alarm    The temperature alarm indicates too high or too low temperature   The alarm will be activated if the temperature rises or falls more than 0 5   C from the setpoint   There will be an acoustic signal and the red LED light in the alarm key will be activated     Press alarm key to mute the acoustic signal    Press alarm key once again to bring the  signal back on        Note The red LED light and the acoustic signal will turn off when the temperature stabilises  to the setpoint level        5 9 Warming up    Warming up from 20 0 to 39  0   C takes less than 20 minutes  under normal conditions   When a cold item is placed on the heated area it may need up to 30 minutes to stabilise   depending on the thermal mass of the object and the initial temperature        Page 7 Kivex Biotec Ltd    Instruction Manual   R65       5 9 1 Safety barrier    There is a greater heat loss from the edges of the warming plate  and the tolerances can not be kept as  precisely in these areas  A high accuracy can only be maintained in the centre area of the warming plate     Please see picture below     Top view                                                    Our general recommendations     Dark grey area only      0 3 degrees Celcius  Light grey area      0 8 degrees Celcius  White area  No heat       Page 8 Kivex Biotec Ltd    Instruction Manual   R65       6  Menu function       The user can access a 
8. NT gs sia ve oe ah eo ds kk ee we aw Be Oe eee a we Fa 14  TES    o SS OS SE ORME ES OHS ESS ee Ee REE SS 14  SESE hess oe YS eters Be a Aw a os Bees a ea ee 15  HEAT    ge 3   69 RR oe eee REE XY Y EW OE ORE YE EE ESRYYIGS 16  PSU nee eee se BAe ey a ee a eae ole eee be 3 17  1910 a 5 oak eS ae i an ha ne dh oe ae a Be es a 18  RESTO o2 ie a ae a aui ES ee wee 19  7  User maintenance   ius usus ue Ge BOS REESE oo SSR Soe ERES 20   hES  o        ceeds eye ce eae a eke eS eee os 20  7 2 Calibration     4 44 4 e022 dS oR hae ee a OS a ee eee ees 20  8  Trouble Shootlliti s ces nas coats eae ES OE CR RS 21  9  Technical data is ohio lok Cate PU XX eae x ec 21  10  Spare pal tS errer irena OS eee te ears eee eke ee kee a 22  10 1 List of spare partnumbers                0 000 e eee eee 22  10 2 Spare parts  explodedview                00 eee eens 23  11  Limited warranty   eerie t RIA EE rx 24  jrAI co po CPP 25  13  Replacement   sss sos pRRER RU X Rex O44 a 25       Kivex Biotec Ltd    Instruction Manual   R65       1  General information and service       COPYRIGHT    This manual contains information that is subject to copyright  All rights reserved  This manual should not  be photocopied  otherwise copied or distributed  completely or in part  without the approval of  K SYSTEMS Kivex Biotec     Users of K SYSTEMS Kivex Biotec products should not hesitate to contact us if there are any unclear  points or ambiguities in this manual        K SYSTEMS   Kivex Biotec Ltd  Klintehoj
9. T T    The INT T  Integral Time  option is for changing the base value for the PID controller    This should not be attempted by unauthorised persons  If set at a different level the controlling principle  will be affected    From the factory it is set to a closely calculated value specific for the model     CAUTION Do not change the value here  If by mistake any changes are made or doubts occur if the  value are correct please see the REST function in the menu for the restore of the defaults       Ti St  The Ti St  Time Set  option is for setting the time     Follow these steps to navigate in the Ti St option     Press and hold UP DOWN key for 3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option  Ti St   appears in the display     Press and hold setpoint key to change  values     While holding the setpoint key in  press the  arrow UP to change the minutes and arrow  DOWN key to change the hour     When the time is set  let go of the setpoint  key    Press and hold UP DOWN key for 3  seconds to exit the menu           Page 14 Kivex Biotec Ltd    Instruction Manual   R65       St St    The St St  Start Set  option is the timer function for the heat   By using this option  time can be saved as the R65 can be ready for use before procedure start  in the morning     Note This function will only work correctly if the clock is set        Follow these steps to navigate in the St St option     Press and hold UP DOWN key for
10. at a low voltage     5 3 Start of apparatus    Connect the Unit to the PSU and the PSU to the mains wall socket   To power up the unit  press the heat on button on the keyboard  The display indicates the current  temperature  either on the table plate or in the drawer     5 4 Keyboard functions    Heat ON OFF  LED Display    Arrow keys UP DOWN          Page 5 Kivex Biotec Ltd    Instruction Manual   R65       5 5 Operating the heated area    Press heat ON OFF key to show the actual  temperature on either the table plate or in  the drawer    Press arrow UP or arrow DOWN to shift  between these temperatures     Temperature on table plate     Temperature in drawer        5 6 Temperature setting and control    The display indicates the average temperature of the surface     Press setpoint key until set temperature is  shown     Press and hold setpoint key     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  raise or lower the set temperature     When the desired temperature is reached   let go of setpoint key        Note The setpoint can be adjusted to a reading between ambient and 49 9  C           Page 6 Kivex Biotec Ltd    Instruction Manual   R65       5 7 Combination keys    Press alarm and UP key  The keyboard is  locked     Press alarm and UP key  The keyboard is  unlocked     Press Heat ON OFF and setpoint key  Switches between  temperature and  time     Press DOWN key and Heat ON OFF  The heat in the  drawer is turned  off     Press DOW
11. avel costs during this period     This limited warranty is exclusive and in lieu of all other warranties whether written  oral  expressed or  implied  In particular  K SYSTEMS Kivex Biotec does not warrant that the product is suitable for the needs  of the purchaser and there are no warranties given as to merchantability or fitness for a particular pur   pose other than the one specified in K SYSTEMS Kivex Biotec literature that accompanies every specific  product  K SYSTEMS Kivex Biotec assumes that the Purchaser is experienced in the use of this device  and is able to judge from his her own expertise the suitability or otherwise of the product for the in   tended use     K SYSTEMS Kivex Biotec reserves the right to change or discontinue this product without prior notice        Page 24 Kivex Biotec Ltd    Instruction Manual   R65       12  Liability       Because K SYSTEMS Kivex Biotec has no control or influence over the conditions under which this device  is used  over its method of use or administration  or on handling of the product after it leaves its pos   session  K SYSTEMS Kivex Biotec takes no responsibility for the results  use and or performance of the  product  K SYSTEMS Kivex Biotec expects that use of the product will be confined to trained and expert  users     In no event shall K SYSTEMS Kivex Biotec be liable for any direct or indirect damages including inciden   tal  consequential or special damages  arising out of or in connection with the use or performan
12. ce of the  product     If K SYSTEMS Kivex Biotec provides you with technical documentation  this does not authorise you to  perform repairs  adjustments or alterations on the device or accessories     No representative of K SYSTEMS Kivex Biotec and no vendor of the product is authorised to change any  of the foregoing terms and conditions  and the purchaser accepts the product subject to all terms and  conditions herein  subject always to any contrary provisions which are necessarily implied by stature or  law notwithstanding the within terms and conditions     13  Replacement       As mentioned in the Limited Warranty  the decision whether to provide any remedy or whether to re   fund any portion of the purchase price shall be at the discretion of K SYSTEMS Kivex Biotec     Before returning a product for any reason  please contact your nearest K SYSTEMS Kivex Biotec distribu   tor for assistance and instructions     Only for all customers in Denmark  Norway and Sweden   please take direct contact with our head office at     Tel   45 4599 5600  Fax   45 4599 5619          Email  info k systems dk          Page 25 Kivex Biotec Ltd    
13. eel without brake 52047       Note When ordering spare parts  remember to specify model  serial number and software  version  To identify software version please refer to 6 1 Overview of options on page 10        Page 22 Kivex Biotec Ltd    Instruction Manual   R65       10 2 Spare parts   exploded view                            52773 US          Page 23 Kivex Biotec Ltd    Instruction Manual   R65       11  Limited warranty       K SYSTEMS Kivex Biotec warrants to the purchasers of all devices and products manufactured by K SYS   TEMS Kivex Biotec  the product was prepared and tested in accordance with good manufacturing prac   tices and guidelines and are in compliance to the CE norms issued by the competent authority     In the event of product failure under normal use  due to defects in material or workmanship  within a  period of twentyfour months from the date of shipment of the Product and from the point of origin  the  product will be repaired  or at K SYSTEMS Kivex Biotec option  replaced  at no charge  This limited war   ranty does not apply to products subjected to abnormal use or conditions  improper storage  damaged  by accident  misuse or abuse  improper line voltage  products whose serial number has been altered  to  products not shipped in accordance with the recommendations of K SYSTEMS Kivex Biotec  and or to  products altered or serviced by anyone other than K SYSTEMS Kivex Biotec authorised distributors  Dis   tributor is responsible for the labour and tr
14. ess and hold setpoint key to change  values     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  select between  ON  and  OFF      When the required setting is activated  let  go of the setpoint key    Press and hold UP DOWN key for 3  seconds to exit the menu        Note A dot will turn on in the right side of display  indicating that the Heat function is active           Page 16 Kivex Biotec Ltd    Instruction Manual   R65       A St  The A St  Automatic Start  option is used to repeat the timer  St St  function every day of the week   Note This function works in conjunction with St St and HEAT     If St St is set to 8 00 and heat function is on  the heater will automatically turn on at 8 00  every day        Follow these steps to navigate in the A St option     Press and hold UP DOWN key for 3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option  A st   appears in the display     Press and hold setpoint key to change  values     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  select between  ON  and  OFF     When the required setting is activated  let  go of the setpoint key    Press and hold UP DOWN key for 3  seconds to exit the menu        Note If this function is set to  on  the heat function will be repeated every day  but if  A St function is set to off    the heat function will only be activated one time           Page 17 Kivex Biotec Ltd
15. moistened with a  diluted neutral detergent       The heated area is able to withstand some water spills  However  precautions are  still necessary if water is spilled on the surface  Unplug the power cord  and then wipe dry  with a dry cloth immediately     Note If water gets inside the unit  contact your K SYSTEMS Kivex Biotec representative to check  electrical safety        7 2 Calibration    For optimal performance the unit should be calibrated once a year by authorized K SYSTEMS Service  provider        Page 20 Kivex Biotec Ltd    Instruction Manual   R65       8  Trouble shooting       Temperature alarm is on  Temperature more than Check the setpoint tem      0 5   C from set tem  perature  Wait for the  perature  temperature to stabilize     No heating  Setpoint below ambient  Change setpoint   eg  37   C    Display remains off when No mains supply  Plug in the power     HEAT    key is activated  connector   Power connector  Reconnect power  not connected  connector   Defective  HEAT  key  Replace the keyboard        9  Technical data    PSU AC input 100 240V 50 60 HZ 1  6 A MAX  DC output 24V  10A   Alarms Audible and visible for  out of range temperature             Page 21 Kivex Biotec Ltd    Instruction Manual   R65       10  Spare parts       10 1 List of spare part numbers    Electronics power and display PCB 51298    Sensor PCB  Keyboard  Cooling profile  RS 232 connector  PSU External power supply   Mains Cable Standard Schuko  Mains Cable   UK   P2007      Wh
16. number of advanced functions via the menu     Press and hold UP DOWN key for 3  seconds to enter the menu     The first option  UNIT  will appear in the  display    Press UP DOWN key to navigate within the  menu     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  change values within the options     Press and hold UP DOWN key for 3  seconds again to exit the menu           Page 9 Kivex Biotec Ltd    Instruction Manual   R65       The menu consist of the 11 options listed below   For further detail see the description on the following pages     6 1 Overview of options    Version 3    E Bs  le  BH     lt   Ld    S    Calib  value     lt   Ld    S    Calib  value       01      s     d A     M 1       lt   Ld     lt   lt   lt   lt   L4  gt  L4 Ld    Software version       Page 10 Kivex Biotec Ltd    Instruction Manual   R65       UNIT    In the UNIT option the displayed temperature unit can be set to either  degrees Celsius or Fahrenheit     Follow these steps to navigate in the UNIT option     Press and hold UP DOWN key for 3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press and hold setpoint key to change  values     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  select the required type of unit     When the required type has been selected  let go of the setpoint key    Press and hold UP DOWN key for 3  seconds to exit the menu           Page 11 Kivex Biotec Ltd    Instr
17. the table top  Tn t  and the  drawer  Tn b     If there is an offset between the value on the display and any measurements made with a high precision  external temperature sensor  this can be corrected     The new temperature reading will be kept as the displayed value with temperature controlling  conducted on this basis     Follow these steps to navigate in the Tn t Tn b option     Press and hold UP DOWN key for 3  seconds to enter the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option   Tn t Tn b  appears in the display     Press and hold setpoint key to change  values     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  select the value of temperature     When the required temperature has been  selected let go of the setpoint key     Press and hold UP DOWN key for 3  seconds to exit the menu        CAUTION Any change in the factory set calibration should always be based on very certain  temperature measurements  K SYSTEMS Kivex Biotec only recommend the use of a high  quality PT 100 sensor that is calibrated with the precision instrument used for measuring  according to the manufacturers specifications  Ensure an optimal contact between  the sensor and the place measured  Use only a sensor type that is correct for the  purpose  and which has the correct size  If in doubt  contact your local distributor or  K SYSTEMS Kivex Biotec           Page 13 Kivex Biotec Ltd    Instruction Manual   R65       IN
18. uction Manual   R65       RS232    The RS232 option is for serial communication and data logging of the temperature  and alarm conditions     Follow these steps to navigate in the RS232 option     Press and hold UP DOWN key for 3  seconds to enter the the menu     The option  UNIT  will appear in the  display    Press DOWN key until the option  RS232   appears in the display     Press and hold setpoint key to change  values     While holding the setpoint key in  press  either the arrow UP or arrow DOWN key to  select between  ON  and  OFF       OFF    The RS232 function is turned off    ON   Data is send once every two seconds     When the required setting is activated  let  go of the setpoint key    Press and hold UP DOWN key for 3  seconds to exit the the menu        Data logging the temperature valves during use     Note The RS232 cable must be connected before start        Follow the steps in the table above and choose  ON    Choose one of the settings to get the required data    Sends the data for the last one hour   Sends the data for the last two hours    Sends the data for the last five hours        Note The use of this function warrants extra equipment software  Ver  3  and on some base  models addition of a Serial PCB  The parameters on or off will not affect normal usage  when serial communication is not connected           Page 12 Kivex Biotec Ltd    Instruction Manual   R65       Tn t Tn b   The Tn t Tn b option is for calibration of the displayed temperature of 
    
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