OZ Biosciences / Protocol Magnetofection™: SelfMag Carboxy Kit vs
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1. for all nucleic acids PN31000 CombiMag 1mL to boost transfection reagent CM21000 SilenceMag 1mL for siRNA application SM11000 ViroMag imL for viral application VM41000 ViroMag R L 1mL for lenti amp retro viruses RL41000 FluoMag P 100uL FP10100 FluoMag C 100uL FC10100 FluoMag S 100uL FS10100 FluoMag V 100uL FV10100 Protein Delivery Systems Ab DeliverIN Antibody Delivery reagent 0 5 mL Al20500 Pro DeliverIN Protein Delivery reagent 0 5 mL PI10500 Tee Technology lipid based reagents Lullaby siRNA transfection reagent LL71000 DreamFect Gold Transfection reagent 1mL DG81000 DreamFect Transfection reagent 1mL DF41000 EcoTransfect Transfection Reagent imL ET11000 VeroFect Transfection Reagent 1mL VF61000 FlyFectin Transfection Reagent 1mL FF51000 Gene amp Protein Tools Bradford Protein Assay Kit BA00100 GeneBlaster Ruby GB20011 GeneBlaster Sapphire GB20012 GeneBlaster Topaz GB20013 B Galactosidase ONPG assay kits GO10001 B Galactosidase CPRG assay kits GC10002 X Gal Staining Kit GX10003 DNA markers 100 bp DNA ladder PF00100 100 bp DNA ladder PLUS PF00200 1 Kbp DNA ladder PF00300 ShortRun DNA Marker PF00400 pBR328 Hinf I Bgl I PF00500 pUC18 Hpa II PF00600 pUC19 MSp I PF00700 pBR322 Hae III PF00800 A Hind III phiX 174 Hae III PF00900 product list complementary information and OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com remember to2. visit our website 7 Purchaser Notification O Limited License The purchase of the SelfMag Carboxy Kit and all included items grants the purchaser a non transferable non exclusive license to use the kit and or its separate and included components as listed in section 1 4 Kit Contents This reagent is intended for in house research only by the buyer Such use is limited to the coupling and delivery of molecules into cells via magnetic nanoparticles as described in the product manual In addition research only use means that this kit and all of its contents are excluded without limitation from resale repackaging or use for the making or selling of any commercial product or service without the written approval of OZ Biosciences Separate licenses are available from OZ Biosciences for the express purpose of non research use or applications of the SelfMag Carboxy Kit To inquire about such licenses or to obtain authorization to transfer or use the enclosed material contact the Director of Business Development at OZ Biosciences Buyers may end this License at any time by returning all SelfMag Carboxy Kit material and documentation to OZ Biosciences or by destroying all SelfMag Carboxy Kit components Purchasers are advised to contact OZ Biosciences with the notification that a SelfMag Carboxy Kit is being returned in order to be reimbursed and or to definitely terminate a license for internal research use only granted through the purchase
3. 0 uL of Buffer C1 by pipetting up and down Repeat steps 3 and 4 one more time Prepare 100 uL of the molecule to be coupled ligand at a concentration of 10 umol L in the Buffer C1 If your molecule is already in a buffered solution we recommend you to dialyse it against water before use since some salts containing buffer could potentially cause the aggregation of the nanoparticles Add between 5 and 50 uL of the ligand solution to the 100 uL of SelfMag prepared previously Then vortex to ensure a good mixing and shake agitator mixer the mixture 15 30 minutes at room temperature Immediately prior to use prepare a 10X stock solution of EDC at a concentration of 10 mg mL in water Then dilute 10 uL of the 10X EDC solution to 100 uL with the Buffer C1 in order to obtain a 1X EDC solution Add 5 to 10 uL of 1X EDC solution 5 to 10 ug to the SelfMag Carboxy nanoparticles ligand mixture Vortex to ensure a good mixing Shake agitator mixer the mixture for at least 2 hours at room temperature or overnight at 4 C depending on the nature of the molecule to be coupled Then proceed to the washing protocol as described below 2 3 2 3 Washing and Storage of Coupled Nanoparticles All coupling procedures require washing of the molecule conjugated SelfMag Carboxy nanoparticles to remove the excess of ligand EDC and the resulting by product isourea 1 Place the mixture microtube in the MagID devic
4. Magnetofection SelfMag Carboxy Magnetic Delivery System Kit Magnetofection SelfMag Carboxy Kit Instruction Manual SelfMag has been designed to couple your molecules of interest onto magnetic nanoparticles and deliver them into cells by magnetic targeting SelfMag Carboxy Kit allows you to make your own magnetic delivery system List of SelfMag Carboxy Kits Catalog Description Content Number of assays Number SC20000 SelfMag Carboxy Kit All in one Kit 1 to 50 coupling reactions 1000 delivery assays SC21000 SelfMag Carboxy nanoparticles 1 mL 1 to 50 coupling reactions SC22000 Buffers Kit C Buffer C1 15mL coupling 1 to 50 coupling reactions Buffer C2 15 mL washing storage 100 mg EDC SF40000 MagFectin 1 mL 1000 delivery assays DM30000 MagID magnetic device 1 unit MF10000 Super Magnetic Plate 1 unit 1 Contains 1 vial of SelfMag Carboxy nanoparticles 1 Buffers Kit coupling washing storage buffers and EDC 1 MagID device 1 vial of MagFectin and one Super Magnetic Plate Contains 1 bottle of coupling buffer C1 1 bottle of washing storage buffer C2 and 1 vial of EDC reagent Use the content of the table above to determine the appropriate catalog number for your needs You can order these products by contacting us telephone fax mail e mail or directly through our website For all other supplementary information do not hesitate to contact our dedicated technical support tech ozbios
5. ciences com OZ BIOSCIENCES Parc Scientifique et Technologique de Luminy BP13 13273 Marseille Cedex 9 France Tel 33 0 4 91 82 81 75 Fax 33 0 4 91 82 81 70 E mail contact ozbiosciences com Site Internet www ozbiosciences com Ww OZ BIOSCIENCES The art of delivery systems OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 1 Table of Contents 1 Technology 2 4 1 1 Product Description 2 1 2 Physical Characteristics of SelfMag Carboxy Nanoparticles 2 1 3 Principle 3 1 4 Kit Content 3 1 5 Magnetic Apparatus 3 1 6 Storage Stability 4 2 Instruction for Coupling the Molecules 4 6 2 1 General Considerations 4 2 2 Activation and Coupling of the Molecules 4 5 2 3 Washing and Storage of Coupled Nanoparticles 5 6 2 4 Scale up down of the Coupling Reaction 6 3 Protocol for delivering Coupled SelfMag Nanoparticles into Cells 6 7 4 Example of Application 7 8 4 1 Protocol for Coupling B Galactosidase 7 4 2 Intracellular Delivery of B Galactosidase Coupled Nanoparticles 7 8 5 Appendix 8 5 1 Quality Controls 8 5 2 Troubleshooting 8 6 Related Products 9 7 Purchaser Notification 10 1 Technology O 1 1 Product Description Congratulations on your purchase of the SelfMag Carboxy Kit SelfMag Carboxy nanoparticles are colloidal monodispersed superparamagnetic which are composed of a magnetic core surrounded by a highly cross linked polymer shel
6. corporating a high energy neodymium magnet It is designed to accommodate standard 1 5 mL tubes and is also suitable for some 2 mL tubes It is ideal for your magnetic nanoparticles coupling reaction and purification It is adapted to working solutions ranging from 10 uL to 2 mL This device allows a quick magnetic separation process lt 5 minutes with a high yield separation Durable and easy to use this device with an open faced design facilitates aspiration pipetting etc e Super Magnetic Plate for delivery into cells As for all Magnetofection reagents the delivery of coupled SelfMag Carboxy nanoparticles is supported by an appropriate magnetic field and the MagFectin delivery reagent The special geometry of the Super Magnetic Plate produces a strong magnetic field that is suitable for all cell culture dishes T 75 flasks 60 amp 100 mm dishes 6 12 24 48 and 96 well plates Warning and Handling These products should be handled with care Avoid direct contact with other magnetic materials and devices Person with pacemakers implants should avoid direct contact Keep loose ferrous material away and do not attempt to disassemble Keep all magnetic media watches and sensitive electronic devices away from these magnetic apparatus Credit cards tape and disks can be erased in the presence of a magnetic field Bodily harm pinching of hands and skin can result if magnets are not handled correctly Maintain distance between t
7. e 2 4 Scale up down of the Coupling Reaction The suggested protocol described above is provided for the coupling of 100 ug of nanoparticles However this protocol can be scaled up or scaled down depending on the quantity of your molecules or on your specific needs Refer to the Table 1 below for suggested quantities of SelfMag nanoparticles molecules to be coupled and EDC Table 1 Quantities of SelfMag nanoparticles molecules and reagents suggested for coupling SelfMag Carboxy Molecule to be coupled nanoparticles 1 mg mL 10 pmol L EBC uka 4 Solution z Solution Solution Solution l Sane volume Salen volume cae concentration volume mul j HL uL mg mL HL 10 100 pmol 5 10 nmol 25 250 pmol 12 5 25 nmol 50 500 pmol 25 50 nmol 0 25 2 5 nmol 0 13 0 25 umol 0 5 5 nmol 0 25 0 5 pmol 3 Protocol for Delivering Coupled SelfMag Nanoparticles into Cells O The instructions given below represent a model of protocol that was applied successfully to deliver coupled nanoparticles into several cells We recommend you to start by following this general protocol as guideline and then optimized the conditions if required Please refer to the table 2 below as a starting point for experimental conditions However some optimization may be needed depending on the nature of the molecule coupled and the cell type 1 The day prior the delivery experiment plate the ce
8. e let stand until the supernatant clears up and remove it by pipetting aspiration leaving the nanoparticles pellet undisturbed Remove the microtube from the MagID device and resuspend the SelfMag nanoparticles in 100 uL of Buffer C2 by pipeting up and down Optionally in order to quench the non reacted activated carboxylic acid groups resuspend the coupled nanoparticles with 100 uL of 1 mM Tris pH 7 4 instead of Buffer C2 then shake the mixture for 15 minutes at room temperature Place the SelfMag microtube in the MagID device let stand until the supernatant clears up and remove it by pipetting aspiration leaving the nanoparticles pellet undisturbed Remove the microtube from the MagID device and resuspend the SelfMag nanoparticles in 100 uL of Buffer C2 by pipeting up and down Repeat steps 3 and 4 one or two times OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 5 The use of MagID device avoids significant lost of nanoparticles during this procedure Consequently 100 ug of coupled SelfMag Carboxy nanoparticles are obtained at a final concentration of 1mg mL Store the coupled nanoparticles at 4 C Coupled SelfMag can usually be stored for several weeks at this temperature depending on the stability of the coupled molecules However it is recommended to use them rapidly after coupling For every delivery experiment resuspend the nanoparticles by pipeting up and down prior to us
9. ent free DMEM culture medium OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 7 4 Add the 100 uL of the MagFectin solution to the B Galactosidase coupled SelfMag nanoparticles and mix immediately by pipeting up and down 5 After 20 minutes of incubation transfer the 100 uL of complexes onto cells The total volume per well DMEM medium complexes is 500 uL 6 Place the cell culture plate upon the Super Magnetic Plate for 20 minutes 7 Remove the Super Magnetic Plate 8 Cultivate the cells under standard conditions during 3 to 24 h 9 The delivery of B galactosidase into cells was revealed using OZ Biosciences X Gal staining kit cat GX 10003 or OZ Biosciences ONPG B galactosidase assay kit cat G010001 Results can be found on our website www ozbiosciences com 5 Appendix O 5 1 Quality Controls To guarantee the performance of SelfMag Carboxy Kit produced we qualify each lot using rigorous standards Components Standard Quality Controls SelfMag Carboxy 1 Quality and size homogeneity of the magnetic nanoparticles Nanoparticles 2 Stability of the magnetic nanoparticles formulation 3 Sterility Thioglycolate assay Absence of fungal and bacterial contamination shall be obtained for 7 days MagFectin 1 Sterility Thioglycolate assay Absence of fungal and bacterial contamination shall be obtained for 7 days Intracellular delivery assay Ma
10. gFectin efficacy to deliver Galactosidase coupled SelfMag nanoparticles on NIH 3T3 cells Every lot shall have an acceptance specification of gt 80 of the activity of the reference lot Buffer solutions and EDC Sterility Thioglycolate assay Absence of fungal and bacterial contamination shall be obtained for 7 days Coupling assay All buffers and EDC are tested for their efficiency to couple p Galactosidase onto SelfMag Every lot shall have an acceptance specification of gt 90 of the activity of the reference lot MagID and Super Magnetic Plate Tests of solidity Test of the magnetic field force 5 2 Troubleshooting Our dedicated and specialized technical support group will be pleased to answer any of your requests and to help you with your coupling reactions and delivery experiments tech ozbiosciences com In addition do not hesitate to visit our website www ozbiosciences com for new products products citations list of cells successfully tested as well as any other update OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 8 Please feel www ozbiosciences com to stay informed on the latest breakthrough technologies and updated on our complete 6 Related Products O free to contact us for all Description Reference Magnetofection Technology Super Magnetic Plate MF10000 Magnetic Plate 96 magnets MF10096 PolyMag imL
11. ge and NOT other buffers 2 2 Activation and Coupling of the Molecules Formation of an amide bound between a primary amino group of the molecule of interest and a carboxylic acid group on the surface of the SelfMag Carboxy nanoparticles is mediated by carbodiimide activation 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride EDC MW 191 7 is the carbodiimide most commonly used Its water solubility allows for direct addition to a reaction without prior organic solvent dissolution The excess of reagent and the by product isourea resulting from the reaction are both water soluble and can be easily removed A stock solution of EDC must be prepared to facilitate the addition of a small molar amount to the reaction mixture and used immediately to prevent extensive loss of activity EDC is not stable in water and thus must be prepared freshly for each reaction OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 4 10 11 Before each use resuspend the SelfMag Carboxy nanoparticles by pipetting up and down or by vortexing 1 minute Avoid foaming Transfer 100 uL of the SelfMag Carboxy nanoparticles in a microtube Place the SelfMag microtube in the MagID device let stand until the supernatant clears up and remove it by pipetting aspiration leaving the nanoparticles pellet undisturbed Remove the microtube from the MagID device and resuspend the SelfMag nanoparticles in 10
12. l The SelfMag surface is activated with carboxylic acid functionality COOH making an ideal reagent for coupling any molecules bearing an amino reactive group in order to produce your self made magnetic delivery system SelfMag Carboxy nanoparticles are supplied in an aqueous suspension The hydrophilic surface of SelfMag ensures low non specific binding excellent dispersion abilities and easy handling in solution Optimized coupling and washing storing buffers EDC for carbodiimide activation a magnetic device MagID for the coupling and washing procedure are also supplied In addition a magnetic plate that generates a magnetic field gradient and a specific delivery reagent MagFectin allowing the transport into cells of magnetic nanoparticles Magnetofection are provided SelfMag Carboxy Kit contains all components to make in a simple and easy way your own magnetic delivery system 1 2 Physical Characteristics of SelfMag Carboxy Nanoparticles Diameter 160 nm CV max 20 Relative density 1 25 1 3 Magnetic susceptibility 35 37 emu g Specific surface area per carboxylic acid functionality 34 38 A2 Iron oxide content approx 50 COOH density 150 umol g Solid content stock solution 1 mg mL 0 1 Beads concentration 2 5 x 101 beads per mL OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 2 1 3 Principle SelfMag Carboxy particles are designed as superpara
13. lls in your tissue culture dish The suitable cell density will depend on the growth rate and the conditions of the cells Cells should not be less than 60 confluent percentage of growth surface covered with cells at the time of delivery see the suggested cell number in the table 2 The correct choice of optimal plating density also depends on the planned time between delivery and analysis for a large interval we recommend a lower density and for a short interval a higher density may be advantageous Table 2 Experimental conditions suggested for delivering the coupled nanoparticles into cells Cells to plate Coupled SelfMag MagFectin diluted solution Tissue solution volume Total pL C 1 ug L 96 well 0 05 0 2x 10 150 uL 0 5 1 0 2 0 5 50 200 uL 24 well 0 5 1x10 400 uL 2 4 0 8 2 100 500 uL Adherent Seeding Cell Number Volume MagFectin Dilution I Dish Cuiture Dis Volume pL Volume pL Volume well 12 vell 1 2x10 800 UL 4 8 1 6 4 200 1 mL 6 well 2 5x10 1 6 mL 8 16 3 8 400 2 mL 60 mm dish 5 10x 10 3 2 mL 20 40 8 20 800 4 mL 90 100 mm 10 30x 10 7 mL 60 120 25 60 1000 8 mL T 75 flask 20 50 x 10 9 mL 70 130 35 70 1000 10 mL 2 Before each use vortex the molecule coupled SelfMag nanoparticles solution Add the suggested amount of nanoparticles see Table 2 to a microtube or microwell U bottom well is preferred to get a better mixing 3 In ano
14. magnetic nanosized polymer particles bearing carboxy reactive groups on their surface for the coupling and the delivery of your molecules of interest into cells by magnetic targeting Proteins peptides oligonucleotides small drugs or other various molecules having reactive amino groups can be covalently coupled directly onto the surface reactive groups COOH of the nanoparticles and maintain their biological activity Thereafter the molecules coupled to the SelfMag Carboxy nanoparticles can be easily concentrated onto the target cells by exploiting the magnetic field force and internalized within a few minutes by using a specific MagFectin reagent dedicated to the magnetic nanoparticles delivery into cells Magnetofection 1 4 Kit Content e 1 vial containing 1 mL of SelfMag Carboxy Nanoparticles 1 mg mL good for 1 to 50 coupling reactions e 2 bottles each containing 15 mL of sterile buffer solutions Buffer Ci SelfMag Carboxy Coupling Buffer Buffer C2 SelfMag Carboxy Washing amp Storage Buffer 1 vial containing 100 mg EDC e 1 vial containing 1 mL of MagFectin delivery reagent good for 1000 delivery assays e 1 MagID Magnetic Isolation Device for the coupling and washing procedures e 1 Super Magnetic Plate for delivery into cells Magnetofection 1 5 Magnetic Apparatus e MagID Magnetic Isolation Device for coupling reactions and washing procedures MagID is made from an injection moulded plastic housing in
15. of the kit This document covers entirely the terms of the SelfMag Carboxy Kit research only license and does not grant any other express or implied license The laws of the French Government shall govern the interpretation and enforcement of the terms of this License Product Use Limitations The SelfMag Carboxy Kit and all of its components are developed designed intended and sold for research use only They are not to be used for human diagnostic or included used in any drug intended for human use All care and attention should be exercised in the use of the kit components by following proper research laboratory practices For more information or for any comments on the terms and conditions of this License please contact Director of Business Development OZ Biosciences Parc Scientifique et Technologique de Luminy BP13 13273 Marseille Cedex 9 France Tel 33 0 4 91 82 81 74 Fax 33 0 4 91 82 81 70 E mail contact ozbiosciences com OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 10
16. on 5 ug to the SelfMag Carboxy nanoparticles B galactosidase mixture Vortex to ensure a good mixing 7 Shake agitator mixer the mixture for 2 hours at room temperature 8 Wash the coupled nanoparticles with Buffer C2 as described in section 2 3 above 9 Finally store the B galactosidase coupled SelfMag in 100 uL of Buffer C2 at a concentration of 1 mg mL The amount of B Galactosidase coupled on the SelfMag was monitored by using the ONPG B Galactosidase assay kit OZ Biosciences cat GO10001 Results showed 0 45 pmol 54 ng of linked B Galactosidase per ug of nanoparticles which correspond to a coupling efficiency of 23 Efficiency can be optimized by varying various parameters such as incubation time B Galactosidase concentration nanoparticles quantity and incubation temperature 4 2 Intracellular Delivery of B Galactosidase Coupled Nanoparticles The instructions given below represent an example of protocol to deliver the B Galactosidase coupled SelfMag into cells The following procedure can be adapted to deliver other proteins or molecules 1 The day prior the intracellular delivery experiment seed 100 000 NIH 3T3 cells well in a 24 well plate in 400 uL of DMEM complete culture medium with serum 2 Mix the B Galactosidase coupled SelfMag nanoparticles Add 2 to 4 uL 2 to 4 ug of B Galactosidase coupled SelfMag to a microtube 3 Dilute 1 to 2 uL of MagFectin delivery reagent to 100 uL with serum and supplem
17. terest on SelfMag Carboxy nanoparticles we recommend to use between 0 5 and 5 nmol of ligand molecules per mg of SelfMag Carboxy The nanoparticles should be used at a final concentration comprised between 0 5 and 1 mg per mL during the coupling reaction However protocols may be adapted to meet your requirements Both molecules and nanoparticles concentration can be adjusted depending on the stock concentration solubility and availability of your ligand Higher nanoparticles concentrations increase the coupling efficiency and higher ligand concentrations increase the number of molecules coupled onto the nanoparticles The suggested protocol described below has been optimized for a specific application the delivery of molecules into cells It illustrates an example using 100 uL of SelfMag Carboxy particles at 1 mg mL 100 ug of beads but can be scaled up and down to suit specific needs The ligand concentration was set to 10 pmol L but can be adapted according to your needs It is recommended to manipulate SelfMag Carboxy nanoparticles under sterile conditions for using with cells Important note The nature and the ionic strength of the buffers for coupling washing and storage of SelfMag are critical to obtain a high coupling efficiency rate and to avoid the nanoparticles aggregation In this context we highly recommend to only use the buffers provided in the kit Buffer C1 for coupling and Buffer C2 for washing and stora
18. ther microtube dilute the MagFectin delivery reagent in serum and supplement free culture medium see Table 2 for volume of MagFectin and dilution volume OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 6 4 Add the MagFectin solution to the coupled nanoparticles and mix immediately by pipeting up and down 5 After 20 minutes of incubation add the complexes coupled nanoparticles MagFectin to the cells The total volumes per well culture medium complexes are indicated in Table 2 6 Place the cell culture plate upon the Super Magnetic Plate for 15 20 minutes 7 Remove the Super Magnetic Plate 8 Cultivate the cells under standard conditions until assays are performed from 3 to 48 h 4 Example of Application O 4 1 Protocol for Coupling B Galactosidase 1 Resuspend the SelfMag Carboxy nanoparticles by pipeting up and down 2 Transfer 100 uL of the SelfMag Carboxy nanoparticles in a microtube and washed them with Buffer C1 as described in section 2 2 above 3 Prepare 100 uL of a B galactosidase solution at 5 umol L in the Buffer C1 4 Add 50 uL 25 ug 0 2 nmol of the B galactosidase solution to the 100 uL of SelfMag prepared previously Then vortex to ensure a good mixing and shake agitator mixer the mixture 30 minutes at room temperature 5 Prepare a 1X EDC solution 1 mg mL in the Buffer C1 as described in section 2 2 6 Add 5 uL of 1X EDC soluti
19. wo or more magnetic units Product should be stored in a dry environment and should be cleaned with a damp cloth and mild detergent when exposed to harsh solvents Do not autoclave OZ Biosciences Protocol Magnetofection SelfMag Carboxy Kit vs 1 4 www ozbiosciences com 3 1 6 Storage Stability e SelfMag Carboxy nanoparticles Storage at 4 C Upon receipt and for long term use store the SelfMag nanoparticles in the fridge The stock solution of SelfMag Carboxy nanoparticles is stable for at least one year at the recommended storage temperature The SelfMag Carboxy beads must be maintained in liquid during storage and all handling steps Drying will result in reduced performance Precaution should be taken to prevent bacterial contamination of the beads Do not freeze the magnetic nanoparticles Do not add anything to the stock solution of magnetic nanoparticles e Buffer Kits and MagFectin reagent Upon receipt and for long term use store sterile buffer solutions EDC and MagFectin at 4 C These reagents are stable for at least 1 year at the recommended storage temperature EDC should be kept dried e Magnetic device and plate They can be stored at room temperature dry environment is preferred away from any electronic informatics or magnetic materials Shipping condition Room Temperature 2 Instruction for Coupling the Molecules O 2 1 General Considerations For coupling your molecules of in
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