Operation Manual
Contents
1. If the sample being tested itself does not contain sufficient adaptability of microorganism taking following measure to obtain inoculating water Inoculation temperature should be around 2041C City sewage usually use residential area sewage after filtering placed in the 20 C incubator for a day take supernatant as inoculating water The sample from the outlet of biochemical treatment structure When industrial wastewater contain refractory organics take the sample from discharge outlet 3 8Km downstream as inoculating water If no such water source take domestication strains measure to culture inoculating water adapting to the sample being tested in the lab we suggest to adopt the following method take the neutralization or appropriate dilution of the sample for continuous aeration add a small amount of fresh water every day at the same time add amount of surface soil garden soil or sewage make the microorganism adapting to the water large number of propagation When appear large flocculus in the water or there is a mutation on the chemical oxygen demand decreased value which indicate adaptive microorganism have been propagated be used as inoculating water Generally the domestication process take 3 8days 6 4 Nutrition Buffer Solution We offer the user compound nutrition buffer reagent the user also can prepare the nutrition buffer solution by themselves the cost can be reduced Method of preparing can refer to HJ502. 5 2009 lt Water Quality Biochemical Oxygen Demand of 5 Days BODT_5 Determine Dilution and Inoculation Method gt 6 5 Preparation of the BOD Standard Solution Under the temperature of 103 C drying the anhydrous glucose CsH 20 and glutamate HOOC CH gt CH CHNH gt CHOO for one hour take each 150mg dissolved in the distilled water diluted to the graduation line in 1000ml volumetric flask the solution concentration is 210 10mg L at the moment they are prepared when needed The solution can also be a small amount of cryopreservation immediately use after melting 7 Determination Make the sample heat or cool to 2041 C according to the following table put sample water inoculation solution nutrition buffer solution nitrification inhibitor into the sample bottle then add the distilled water to the total capacity graduation line Put the potassium hydroxide into the seal cup through the four holes on seal cup carbon dioxide contact with Il potassium hydroxide particles put seal cup into the bottle mouth make the bottle mouth completely seal then put the BOD test bottle into the measuring instrument tighten the bottle cap put into constant temperature incubator turn on power supply to determine with the change of time appear a curve on the display the curve can be directly read out the BOD value at different time Range mg L 0 Sample Water ml Inoculation solution adding amount ml Nutriti
3. Laboratory Equipment Manufacturer a www mrclab com CE 2 Net a THE STANDARDS INSTITUTION OF ISRAEL User manual 7 j A t P f Es l me MN e iiz all d i eg il e L e AN x z f SC EN Re dE VW e kb A i L S i e I 2 SC E Contents l Prineiple OF E Ee WEE 3 EE ee 3 Se EE e EE ee 3 Eugene 4 SE 4 SREL EE 4 5 2 The EE 5 Se EE EE 5 Sek WOE KINO VCC eebe ees 5 Eeer 6 5 32 LOD wis Sire ieee Gn Gielen ae eee 8 Di DIE AE ee cnet tate heed Sicsencbsseey Prt parece eve seed hcsanciiveds Raw ieee a es 8 SEENEN eege H SE E EE H 6 Preparation for measurement een 10 Gall eet 10 6 2 Water sample pretreatment AE a EEEE 10 ATAS EPE E 10 6 2 2 Disposine chlor E 10 6 2 3 e Ee tempe E EE 10 6 3 Inoculation Le ee E 11 SE Aumin Baer SoUo EE 11 6 5 Preparation of the BOD Standard Solution ccc ceeeeceeeeseseeeesseessssssssssssssssssssssssseaeas 11 ROTTA E 11 Eyal atine the EU 12 1 Principle of Operation The instrument is designed by the pressure difference method simulating the degradation of natural organic matter 21 of the oxygen in the air on the top of Test Bottle continuously replenish the dissolved oxygen consumed in water carbon dioxide CO2 produced in the process of organic matter degradation is absorbed by Sodium hydroxide coming from sealed cap pressure sensor monitor constantly the pressure change of sample bottle There is a correlation betwe
4. en Biochemical Oxygen Demand BOD correspond to the consumption of oxygen in the test bottle and Gas Pressure the instrument deal with such relevance display directly Biochemical oxygen demand BOD value on the screen 2 Specifications Range 0 to 35 0 to 70 0 to 350 0 to 700 mg L without dilution P snegege Resolution 0 1 mg L 0 35 0 70 Img L 0 350 0 700 The minimum dimension requirements of Biochemical incubator 35 x40 x35 cm 3 Features Display the trend curve Printer output Ling to the PC through USB interface Mercury free devices to ensure the accuracy and safety of the BOD measurement Real time clock display Automatic collection and storage of data BOD curve for a user to view Link to the computer to print process curves No parameter and data loss after suddenly electricity cut off LCD humanization interface display easy to learn easy to operate 1 30 days of the testing time setting by the keys Store data and update the result observe the change rate of BOD in the screen during any testing time store 240 data points and 20 groups data of each sample Conform to the standards for environmental protection HJ 505 2009 4 Unpacking the instrument Check each component according to the following list BOD 5 Tester 1 x Power line 1 x Communication cable 1 x CD 6 x Seal Plunger 6 x Sample bottles 6 x Magnetic Stirrer 1 x Sampling spoon for potassium hydro
5. o do the experiment make sure that biochemical incubator is switched on the power supply ahead of 8h and normal operation under20 C constant temperature 6 1 2 In the case of constant temperature put dilution water dilutial water and inoculative dilution water into the incubator for reserve 6 2 Water sample pretreatment 6 2 1 Adjust PH If the sample or diluted sample PH isn t in the range of 6 8 using 0 5mol L hydrochloric acid solution or 0 5mol L sodium hydroxide solution adjust PH to 6 8 The preparation of hydrochloric acid solution 0 5mol L put 40m1 p 1 18g m1 hydrochloric acid into water diluted to 1000ml The preparation of sodium hydroxide solution 0 5mol L put 20g sodium hydroxide into water diluted tol000ml 6 2 2 Disposing chlorine Usually place the sample water containing small amount of free chlorine 1 2 hours free chlorine can disappear For the sample water free chlorine can not disappear in a short period by adding right amount sodium sulfite solution to remove the free chlorine 6 2 3 Influence of temperature In order to expel the supersaturation dissolved oxygen in water the sample water from a lower temperature water or eutrophic lake should heating up to 20 C rapidly or cause the result on the low side The sample water from high temperature water or wastewater outfall should cool down to 20 C rapidly or cause the result on the high side 10 6 3 Inoculation Solution
6. on buffer solution adding amount particles Nitrification inhibitor 1 remark3 Total volume ml 95 Dilution factor Potassium hydroxide adding amount particles Remark 1 Generally add 20ml inoculation solution Necessary to adjust the amount of inoculation solution so as to obtain the best result 2 If the water sample to be tested in itself contains sufficient microorganisms You can not join the inoculation solution and the sample volume is the total capacity dilution factor ts 1 3 If a sample contains nitrifying bacteria maybe occur nitration reaction need to add a nitrification inhibitor Correction Result BOD correction value BOD instrument reading x Dilution factor 8 Evaluating the measuring result A curve by daily measured value intuitively indicating the biochemical effect according to the curve we can make a reasonable judgment as follow 12 Get ll ll O 1 2 3 4 5 6 7 8 9 10 Day Curve Select the smaller measuring range When the sample BOD range is unknown according to the determined value of the total organic carbon TOC permanganate index IMn or chemical oxygen demand CODc and according to the ratio of BODTS5 and TOC IMn or CODcr on the following table estimate BODTS5 expected value BODTYTOC POD BODT CODc L228 LL 0 35 0 65 Biochemical treatment 0 20 0 35 wastewater Curve 2 the curve indicates the beginning of nitrification U
7. osing menu the cursor located in Start at this moment press OK select No 1 channel Picture as follows Press OK display the range selection select the range by Up or Down Arrow Key For example select 350mg L as follows Press OK finish the starting work for Sample 1 channel start to work As follows There is an hour s delay here After an hour enter the running state As follows 350mg L NO 1 280 me L 02 00 00 350meg L 7 05 00 00 310 mg L Stop no working or finish a cycle will be appear this interface After finishing the setting of Sample 1 then set up others by the same method 5 3 3 Stop The running channel can be set to stop Through put the cursor on the channel which you plan to stop press OK the channel will turn into the white background this tell us that the channel will stop 5 3 4 Date aiter Press Menu display Choosing menu the cursor located in Start at this moment press Down Arrow until Date aiter Press OK as follows change date and time by Up or Down Arrow Key and OK 5 3 5 Data printing Connect the computer to print each channel process curve 5 3 6 Check Channel After finishing the setting of each channel and in the running state we only need to press the number key 1 6 checking the running situation 6 Preparation for measurement 6 1 Preparation for experiment 6 1 1 When you start t
8. sually after 5 days typical wastewater occur organic nitrogen biological oxidation The development of nitrifying bacteria is slower than other types of bacteria However some samples contain a high concentration nitrifying bacteria will cause very fast nitrification phenomenon Adding one 13 nitrification inhibitor ally thiourea can control the nitrification problem Curve 3 If the sample water can not contain enough bacteria or lack of regulation means biochemical process lag need to increase the inoculation solution Curve 4 The negative at the beginning indicating the sample of initial temperature is lower than the limited range 20 1 C Oxygen supersaturated samples will also show this Curve Curve 5 the curve indicates there is a leak If the leak 1s slight oxygen consumption curve trend is not obvious the error 1s large If there is such a reaction please check whether the sealing cup and the sample bottle are polluted or damaged MRC LTD HAGAVISH 3 HOLON ISRAEL Tel 972 3 5595252 Fax 972 3 5594529 E mail mrc mrclab com e Web Site www mrclab com
9. xide particle Ey s N k eg eg Ek N fe gt e bw a basch e EE ae gt E Wm SS F K Ka s a EA i R Ki ge 5 Operation 5 1 Keyboard instruction Channel According to the relate channel selection key display selection key 1 6 Data corresponding to the 6 sample bottles Up or Down Selection function key set addition and subtraction ae Menu Menu key press this key to select Starting Work Data Printing Mew Working Cycle System Date 5 2 The main interface Display interface after starting up the instrument In the running state the background of Nol to No 6 is white 5 3 Menu Press Menu display Choosing menu you can select Start Data Printing Working Cycle Date ater Stop and Language The selection menu interface as follows 5 3 1 Working Cycle Press Menu display Choosing menu the cursor located in Start at this moment press Down Arrow until Working Cycle Press OK Through Up or Down Arrow keys to select a working cycle can be set to Sday 7day or 10 day press Ok to finish setting Setting up Working Cycle must be ahead of setting up Starting Work If you set up Starting Work firstly Working Cycle will not able to set up interface as following 5 3 2 Start Setting up Working Cycle then set up Start Press Menu display Cho
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