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TProfessional Basic user manual

1. esse 32 ooo S 27 PAUSE nuunneanennnnnnnnnnnnnnnnnnnnnnnnnnnenn 28 44 BUICK SILIO ne 42 ENE 28 spread sheet mode 38 Se nenne hund imd ee 41 cla 45 temperature increment 41 time increment eene 40 Program MENU eu 19 Ke RERO NEUEN 64 Safety precautions rrrnrrnnrrnnrrnnrnnnrrnnennre 8 Screen SAVES nennen nen 50 Self test extended een 53 Power on self test 53 Service re 52 Service info file eee eeeeeeeeeeceeeeeeeeeeees 54 Servicing ee 64 Short manual 57 e EZ ER EE 41 0 EE EETA 45 SUperviSOr cccceecceeeceeeeeeeeeeeeeeaees 23 55 System configuration 48 OY SSM NO sine 51 System MENU susanne 19 Sek E ln e EE 4T Technical specifications 6 Time and Date 50 User account TEU 22 delete eege ee 23 55 56 User Account Y gt RH 48 User INSTT e 17 Warranty errorrrorrrnrernrrnnrrnnrnnernnernnernnnnnnnr 12 73
2. Insert Step To delete the highlighted step press Soft Key Insert Del User FRA 12 01 06 Edit program FRA 03 demo a 16 01 06 Block type Gradient 96 Preheat Lid ON Delete step no 47 Confirm deletion of step number 4 with Soft Key YES Instruction Manual TProfessional Basic 06 201 1 35 Biometra An Analytik Jena Company ser FRA 2 01 06 Edit program FRA 03 demo a 16 01 06 Block type Gradient 96 Preheat Lid OFF 99 C p l 200 0033 30 0 00 30 150 Pause The total step number is reduced by one the loop now contains 3 steps only 5 4 3 Copy program Press Menu Button Program and enter User directory Move cursor to the program you want to copy User FRA 01 06 Select program name or enter program number gt HE FRA Frank 3 Programs 01 demo 16 01 06 W ssen 03 demo23 16 01 06 Grad 04 free 05 free 06 free Program Program Program Program Press Soft Key Copy Program The cursor automatically jumps to the next available free program store 36 Instruction Manual TProfessional Basic 06 2011 User FRA 2 01 06 Copy program FRA 02 demola to HE FRA Frank 3 Programs 01 demo 16 01 06 I 02 demaia 15 01 06 I 03 demoaza 16 01 06 Grad demola 2 05 free 06 free Fa ME Biometra An Analytik Jena Company The program copy can be stored at any storage number To change program number move
3. e 35x 3 L 4 5 f 2 To reduce or increase the annealing temperature from step to step within a loop set a negative or positive temperature increment under AT C 2 To increase or reduce the incubation time from step to step in a loop set a positive or negative time increment under At s 2 By default the ramping rate heating and cooling is pre set to 3 C sec To adjust the ramping rate enter any value between 0 1 C and 3 0 C under A C s 2 For gradient enabled units only To define a gradient A enter the two temperatures of the block left and right side separated by a minus under C e g 40 60 or B press Gradient and then Edit linear Gradient This activates the screen for programming linear gradients Instruction Manual TProfessional Basic 06 201 1 59 Biometra An Analytik Jena Company User HD 04 07 08 Edit program HD 01 holger 03 07 08 Edit linear gradient in step 37 6 Annealingtemp 55 0 C Row Di Increm 2 Enter an annealing temperature The set annealing temperature defines the temperature in row 6 of the thermoblock Enter a temperature increment Incr The corresponding temperature profile is shown for each single row 2 Press Save Save as to save a program User HD 19 02 08 Tip A folder with common protocols could be no name established From the common folder each user 02 free may copy any program into the own account and d 03 free modify it easily 04 f
4. A log file of the power on self test is stored in the Thermocycler memory see section 6 4 1 Instruction Manual TProfessional Basic 06 2011 13 Biometra An Analytik Jena Company 4 Operating elements 4 1 TProfessional Basic Thermocycler front view Lid pressure adjustment wheel j Lid open A B button Se Ge A Er AN Display Start Stop Keypad Button 4 2 TProfessional Basic Thermocycler rear view t at i R232 serial Port f Block release EN lever Mains plug Power on off Instruction Manual TProfessional Basic 06 201 1 14 Biometra An Analytik Jena Company 4 3 The TProfessional Basic control panel Menu Buttons LED Professional box CS zy w b Je a Keypad hermocycler m CEA Block eteren RA 03 demoa 1000 Heizdeckel 99 C Blocktyp Gradient 96 EF Ak Ak Status sed x m T oB Jae x B 19 D wwz L Jb JE o Run oo 9 36 I 40 0 60 0 60 ES 30 Eee soo 00 30 2 24 m 15 0 pause tr ES Start ry CODEC JEC JCI v Stop S Back Soft Keys Navigation 4 4 High Performance Smart Lid HPSL To achieve optimum pressure on the tubes the TProfessional Basic is equipped with a height adjustable heated lid Close the lid After the samples have been placed in
5. Biometra will repair or replace each part which is returned and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused 12 Instruction Manual TProfessional Basic 06 201 1 16 Subject Index Autoresiart 62 5125 48 Block Men 18 Go 0 T oe 55 GONE EE EE 49 Control poanel sees 15 Declaration of Conformity 71 Decontamination Certificate 68 Defragmentation cccccccsececseeeeeeeeeees 50 DE induta UDIN 70 FT 45 Firmware update o onnanonnnnnnnennnnennennnnn 64 A E pi MR TUE T 31 linear Gradient 32 Heated lid 15 25 26 FI ME ro NE ERR E 62 Language av 47 56 oleh i See 53 54 Koo E 20 Log in Menu 18 Mammtenance 64 Operation Voltage 12 FAG UI E 67 DIN Code 21 47 55 Plasticware arrarnnrrnarnanonnrnannnnrnarnnnnnennn 65 Program EN 45 CON NN 40 soo EE 36 create s 23 deiere 37 delete eren 35 2 a REES 33 SAGEN een 31 graphical mode 38 heating rate eeseeeeeeeeee 40 insert sten 33 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company linear Gradient
6. An Analytik Jena Company ser FRA 12 01 06 Select program name or enter program number gt HE FRA Frank 5 Programs 02 demolb 15 01 06 03 demo23 16 01 06 Grad 04 demo3a 15 01 06 05 demo33 16 01 06 Grad 06 free w Pragrarm Program Program Program Select directory and program with cursor keys and press Start Button 5 6 2 Quick start programs from the block Menu The TProfessional Basic provides the last 5 programs that haven been run for quick start Push the block Menu Button above the Display ser FRA 12 01 06 Block free Block type Unknown Latest programs edited run by FRA 1 FRA 02 demoib d pu Program Select one of the last five programs with the cursor keys Press the illuminated Start button on the left side of the Keypad see section 4 3 page 15 5 6 3 View program prior to start To check the program prior to start press the View Program Soft Key 42 Instruction Manual TProfessional Basic 06 201 1 Jaer FRA 12 01 06 View program FRA 02 demolb 16 01 06 Block type Gradient 96 Preheatlid ON 99 C 55 matt 400 0 30 150 jPase The current program is shown Press View Graph Soft Key for alternative display View Table 12 01 06 View program FRA 02 demoib 15 01 06 User FRA 12 01 06 View program FRA 02 demoib 16 01 06 Biometra An Analytik Jena Company View Graph Block type Gradient 96 Preheatlid OM 99
7. Edit program FRA 03 demo a 16 01 06 Block type Gradient 96 Preheat Lid OFF 99 C as Joost 300 0 30 40 0 60 0 00 30 50 0 150 jPase gt To insert a program step press Soft Key Insert Del The step at which a new step will be inserted is highlighted Instruction Manual TProfessional Basic 06 2011 33 Biometra An Analytik Jena Company ser FRA Edit program FRA 03 demo a cS Block type Gradient 96 Preheat Lid OFF 99 C Ls qos 300 nal 00 soo options 150 Pause Insert Step Edit program FRA 03 demo a 16 01 06 Block type Gradient 96 Preheat Lid ON Insert step before step no 4 Confirm with Soft Key Yes Enter temperature and time settings for new step Jaer FRA 12 01 06 Edit program FRA 03 demo a oe Block type Gradient 96 Preheat Lid OFF 99 C ar BEE 300 mal 40 0 60 0 00 30 gt 150 Pause 34 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company Note The new step has been entered within an existing loop this loop now consists of four steps 5 4 2 Delete program step Move cursor to the step that should be deleted and press Soft Key insert delete step User FRA 12 01 06 Edit program FRA 03 demo a 16 01 06 Block type Gradient 96 Preheat Lid OFF 99 C ip l WELLEN RECH 300 Tool 20 0 TTT options 150 Pause
8. c Block type Gradient 96 400 Joos DO 30 UO 20 30 20 0 15 0 Se ER Pause a5 Program Note In the view program mode a program cannot be edited To edit program press Program Menu Button above the Display 5 6 4 Display during operation After the instrument has been started the below display is shown Instruction Manual TProfessional Basic 06 201 1 43 Biometra An Analytik Jena Company Program addi r 02 demoib User Frank FRA Block type Gradient 96 Remaining Time 1h 22m Estimated End 4 22 pm To show detailed information about the running program press one of the Soft Keys Show Graph and Show Table There are three different display modes available during the run Show Time Show Graph Show Table User FRA 12 01 06 User FRA 12 01 06 2122 User FRA 12 01 06 S p Frogram Frogram Block active 702 demoib Fedde r 02 demoib Block active 02 demoib User Frank FRA Block 20 0 C step 01 05 05 sen Block type Gradient 96 Remaining Time 1h 22m 15 0 Estimated End 4 22 pm Pause CT a5 EE End Lid 99 0 C he a LI pem 5 6 5 Pause Program during run To pause program press Soft Key Pause Block Frogram FRA O02 demolb FREE 2 3 400 IL 4 son S 150 Lid 99 0 C 44 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company The message PAUSE is shown in
9. 2011 Biometra An Analytik Jena Company General Information for Decontamination Please contact your responsible health amp safety officer for details Use of radioactive substances Please contact your responsible person for details Use of genetically change organism or parts of those Please contact your responsible person for details Instruction Manual TProfessional Basic 06 2011 69 Biometra An Analytik Jena Company 13 Note for the disposal of electric electronic waste Note for disposal of electric electronic waste Hinweis fur die Entsorgung von Elektroaltger ten du traitement des d chets des appareils lectrique lectronique This symbol the crossed out wheelie bin means that this product should be brought to the return and or separate systems available to end users according to yours country regulations when this product has reached the end of its lifetime Renseignement For details please contact your local distributor This symbol applies only to the countries within the EEA EEA European Economics Area comprising all EU members plus Norway Iceland and Liechtenstein Dieses Symbol die durchgestrichene Abfalltonne bedeutet dass dieses Produkt von der Firma Biometra f r eine kostenlose Entsorgung zur ckgenommen wird Dies gilt nur f r Ger te die innerhalb Deutschlands gekauft worden sind Kontaktieren Sie f r die Entsorgung bitte die Biometra Service Abteilung Au erhal
10. DD 0 0 00 0 0 9 10 11 12 Save As Enter the desired annealing temperature and define a temperature increment Incr between the single rows of the heating block E g the entered annealing temperature corresponds to the temperature in row 6 of a 96 well block The temperatures for all other rows are calculated automatically The temperature in row 01 left side of the block is the lowest and the temperature in row 12 right side of the block is the highest 32 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company User BS Edit program BS 01 Block type Gradient 96 Step 1 1 Temp Row 01 40 0 C Temp Row 12 ge ERE 5 4 Edit programs Programs can be edited in the Table or Graphical mode Graph To change between these two modes click on the Soft Key Table or Graph respectively The graphical mode is intended for easy modification of existing programs which can then be saved under a new program number and name To edit programs in the graphical mode see section 5 4 5 page 38 Note During operation of the Thermocycler the active program can be viewed but not modified If you want to change settings of the active program for further experiments you have to save a copy of this program to another memory For further information about copying programs see section 5 4 3 page 36 5 4 1 Insert program step Move cursor to the position where a new step should be inserted er FRA 12 01 06
11. ENE NE 49 6 2 3 SEL UNE AN al a ee ea 50 6 2 4 D fragment MEMONy nee nee 50 6 2 5 SCIEEHSAVER EE 50 6 3 gt CC Go 0 ee ee a ee EE 51 6 3 1 EE ee ee een ee ee 51 6 3 2 View Log files of the last five runs rrrarrnanrrnnnrrnrrrnnrrnanrrnnnnranrnnnnrnannnnnennnnennnne 52 6 4 I6 M 52 6 4 1 View log files of Power on self test 53 6 4 2 Execute extended self test 53 Instruction Manual TProfessional Basic 06 2011 3 Biometra An Analytik Jena Company 6 4 3 View log files from extended self test 54 6 4 4 VETT IOO MES eiren Ea E E E EE 54 6 4 5 Create Info file for Biometra Service Department esee 54 6 5 How to contact Biometra ccc ccccccccececeeceece eee eeeeeeeeceeeeeeseeseccueceeeseeseeeeseeseeeseeees 55 6 6 Supervisor system Confguraton cece eccceececeeeeaeeeceeeeeeeeseeeeeeeseeeeseeeseeeseeeeaaeesaees 55 6 6 1 ETEN 55 6 6 2 Delete user account 55 6 6 3 CHANGE SUS S OT IN ve 55 6 6 4 SAN EE 56 6 6 5 Delete all USEF accounts unse 56 Tol 57 TOER 62 8 1 SIOW heating and COOL edle I T 62 8 2 PROBS ee A ee era 62 8 3 Autorestart due to unrecognised power failure rrrrarrrrnrrranernernrarernnrrnnnrrnannnnnennnnnn 62 8 4 Adaptation of protocols from other cyclers cccccececeeeeeeeeeeeeeeeeeeeeeeeeeeteeteeeeeeenes 62 8 9 Releasing wheel in case of blocked lid 00220022000220 nenn nenn n
12. FRA 12 01 06 Save program Select program number I C3 FRA Frank 1 Programs no name d 2 free 03 free 04 free 05 free 06 free my sf Biometra An Analytik Jena Company The first available program number is automatically pre selected Use cursor keys or enter two digits to move to a different program store To enter a program name press button edit name User FRA 12 01 06 Save program Select program number mM HE Enter program name Maximum 15 letters demo CANCEL Enter program name and confirm name with button ok Instruction Manual TProfessional Basic 06 201 1 29 Biometra An Analytik Jena Company User FRA 2 01 06 Save program Select program number a ep I C3 FRA Frank 1Programs DLP demo d D2 free 03 free 04 free 05 free 06 free Confirm program store number with Soft Key Save User FRA 2 01 06 Select program name or enter program number gt HE FRA Frank 1 Programs 02 free 03 free 04 free 05 free 06 free Program Program Program Program Program number name and date are shown in the file directory To run this program press Menu Button block above the display 30 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company ser FRA 12 01 06 Block free Block type Unknown Latest programs edited run iid FRA 1 FRA 01 demo d Buy Program
13. adapted otherwise drop outs due to unsufficient denaturation or unsufficient primer annealing may occur Biometra recommends the following protocol templates for the optimization of dwells for the TProfessional Basic Thermocycler Instruction Manual TProfessional Basic 06 201 1 45 Biometra An Analytik Jena Company Dwell 15sec Annealing temperature 60 C Dwell 5sec Annealing temperature 60 C 95 C 5 00 95 C 5 00 96 C 00 15 97 5 C 00 05 59 C 00 15 35x 57 5 C 00 05 35x 68 C 00 30 68 C 00 30 72 C 01 00 72 C 01 00 16 C Pause 16 C Pause Dwell 10sec Annealing temperature 60 C Dwell 1sec Annealing temperature 60 C 95 C 5 00 95 C 5 00 96 5 C 00 10 99 C 00 01 58 5 C 00 10 35x 56 C 00 01 35x 68 C 00 30 68 C 00 30 72 C 01 00 72 C 01 00 16 C Pause 16 C Pause The protocol templates are pre installed in the directory Biometra Shared see chapter 5 3 1 Note The given annealing temperatures correspond to a calculated primer annealing temperature of 60 C If necessary the annealing temperature has to be optimised for each single primer pair Biometra recommends to optimize the annealing temperature by a gradient at 0 5 C steps on a gradient enabled PCR Thermocycler Note For the protocol templates an elongation time of 30 sec each is given For optimisation of the elongation time please take the processivity bases sec of the Fast PCR polymerase given in the supplier handb
14. are English and German second software release 6 1 2 Change individual PIN The option allows changing the PIN of the current User Instruction Manual TProfessional Basic 06 2011 47 Biometra An Analytik Jena Company 6 1 3 Delete Account Any user can delete his or her personal account 12 01 06 1 System Configuration User select topic 1 1 Set language Delete account of Frank FRA Warning All programs in user directory FRA will be deleted Important Deleting a User account will automatically delete the user file directory together with all programs stored in this directory Prior to deleting an account ensure that the User directory is empty or save a copy of the programs for further use to a different User directory see section 5 4 3 6 2 System configuration This option allows setting of the signal tone beep Display contrast and time settings User FRA 12 01 06 2 System Configuration select topic 2 1 Configure beep 27 Configure contrast 2 3 Configure time date 6 2 1 Configure beep To switch beep on off toggle between ON and OFF and confirm with Soft Key Set Beep 48 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company ser FRA 12 01 08 2 1 Configure beep Beep Beep Bech Overy jew 6 2 2 Configure Contrast The Display contrast can be set individually for each User Iser FRA Contast Cont ast Change settings with Cursor keys
15. may use pre installed program examples see chapter 5 3 1 or create new programs without a template see chapter 5 3 2 Instruction Manual TProfessional Basic 06 2011 23 Biometra An Analytik Jena Company 5 3 1 Create program from template Log in to your personal user directory and press menu button Program above the display to enter the TProfessional Basic program menu In the directory Biometra Shared you will find several pre installed protocols for different PCR applications Esailkzg us G E EE E Jk Jos E dh ww Net ca dC JC JC C Professional basic her mocyc ler Press softkey Open directory to open the directory Biometra Shared Choose a suitable program using the cursor keys or enter the corresponding program number Protocols may be started directly from the Biometra Shared directory or may be copied to the respective user directory and edited see chapter 5 3 Note Please always before starting a template protocol check the single steps and adapt details times temperatures loops temperature and time increments according to the required PCR conditions respectively Biometra will not be liable if the PCR does not work if necessary adjustments have not been programmed Note The directory is protected by a PIN Code The default PIN for the directory Biometra Shared is 0000 5 3 2 Create program without template Press menu button Program above
16. the block close the lid Turn the wheel clockwise until you hear a clicking noise In this mode the pressure will not increase further even when you keep on turning the wheel Note The pressure of the lid has been optimized for a fully loaded block If only very few tubes are loaded to the block you should place two dummy tubes each in the four corner positions to avoid damage of tubes by excessive pressure OOOQOO OOOQOO0 J OOOQOO ODDO DO EO OOOOO OOOQOO OOOOO Open the lid First Release pressure by turning the wheel counter clockwise As soon as there is no more resistance the pressure has been released Then Open the lid with by pushing the front button Instruction Manual TProfessional Basic 06 201 1 15 Biometra An Analytik Jena Company Important The lid should not be opened under pressure because this leads to damage of the locking mechanism 16 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 5 Operating 5 1 The TProfessional Basic User Interface The TProfessional Basic User interface provides four Menu Buttons above the screen and four Soft Keys below the Screen Menu buttons pe bei gt feed Edit program QUE 01 biometra 29 01 03 la 5 a Zs Je d et Jo JE m nr t SE ER 5 1 1 TProfessional Basic Menu Buttons The four MENU BUTTONS allow quick access to the TProfessional Basic Main Menus These are e Login out Menu e Sys
17. the display and alternates with the elapsed time 5 6 6 Continue program To continue paused program press Soft Key Continue 5 7 Stop program To stop the active program press stop Button ser FRA 12 01 06 Pragrarm addi FRA 02 demoib Stop active program To abort the active program confirm with Yes 5 7 1 Stop Program from a pause If the last step in a program is a pause the program has to be stopped by pressing the Stop Button before switching off the instrument Note If the instrument is switched off during a pause step this will be recognized as a power failure a referring error message is written to the log file 5 8 Optimization of Fast PCR programs The usage of Fast PCR Kits allows a significant shortening of PCR protocols in comparison to normal programs when conventional polymerases are used Though suppliers of Fast PCR kits suggest some protocols for their polymerases for the optimization of Fast PCR protocols some physical factors have to be considered E g the temperature transfer from the thermocycler block into the reaction mix is hindered by low temperature conductivity of the plastic of the reaction tubes Moreover the temperature spread within the block especially at short dwells depends on its geometry and is different between various block types To reach the desired temperature in the reaction tube when dwells are shortened lt 15 sec the target temperature of program steps has to be
18. value from cycle to cycle 40 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company Note A time increment will increase the total runtime depending on the numbers of cycles and the size of the increment A program with many cycles and large time increments will take a significantly longer time than a standard protocol 5 5 3 Temperature increment or decrement gt AT C For some applications it is useful to start with a higher temperature and to decrease this temperature from cycle to cycle This subsequent lowering of a temperature is called touch down To decrease a temperature from cycle to cycle enter a negative temperature increment in the row labelled with AT C Note Be sure that the temperature decrease is set in a step that lies within a loop Otherwise there will be no iterative temperature decrease 5 6 Run program To start program press Start Button see section 4 3 page 15 This button is only active when a program for start has been selected This is indicated by the green LED in the Start button 5 6 1 Select program for start from User directories Any program in the cycler memory can be used to start the thermocycler In addition to the programs in the own User account also programs from other User directories can be started To select a program for start press Program Menu Button above the Display Instruction Manual TProfessional Basic 06 2011 41 Biometra
19. Biometra An Analytik Jena Company Professional basic Thermocycler Instruction Manual Ver 11 06 Model Order No TProfessional Basic Gradient 070 601 TProfessional Basic 070 701 TProfessional Basic XL Gradient 070 602 TProfessional Basic XL 070 702 Please read these instructions carefully before using this apparatus Biometra GmbH Rudolf Wissell Str 30 Service Department D 37079 G ttingen Rudolf Wissell Strasse 14 16 Tel 49 0 551 50 68 6 0 D 37079 G ttingen Fax 49 0 551 50 68 6 66 Tel 49 0 551 50 68 6 10 or 12 email Info biometra com Fax 49 0 551 50 68 6 11 Internet http www biometra com email Service biometra com Biometra An Analytik Jena Company This document describes the state at the time of publishing It needs not necessarily agree with future versions Subject to change Instruction Manual TProfessional Basic 06 201 1 1 Biometra An Analytik Jena Company Contents 1 MONNA 5 1 1 FEN 5 1 2 else ER nennen nennen 9 1 2 1 TProfessional Basic Thermoblock Rene nnne nennen nennen 5 1 2 2 TPB 5 1 2 3 TProfessional Basic User mtertace san snnnsnnn nennen nnnennnenneen 5 1 2 4 TProfessional Basic Housimg 00000 nnnn onen nenn nano nenn anne nnns 5 1 3 Technical epectcatons esse nenn nensis nennen 6 1 4 LENE eee T 1 4 1 PCR License Legal Dscamer enne T 1 4 2 COP ee ee EE 7 1 4 3 PAO T 7 1 4 4 Meaning of th
20. Press the illuminated Start Button on the left side of the keypad 5 3 10 Set Gradient The easiest way to define a gradient is to enter the two temperatures of the block left and right side separated by er FRA 12 01 06 Edit program FRA 01 demo 16 01 06 Block type Gradient 96 Preheat Lid OFF 99 C Joel E _ 300 00 30 Confirm with Enter or move cursor to the next field Alternatively move cursor to the field for temperature setting and press Soft Key Gradient 31 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company Edit program B5 01 Block type Gradient 5 Step 1 1 Temp Row 01 40 0 C Temp Row 12 m 2 Gradient In the Gradient Screen you can enter a new or edit an existing Gradient Enter new settings for Row01 left side and Row12 right side and confirm with Enter After both settings are confirmed with Enter the temperatures for the single rows are displayed in the bar chart A With the cursor keys UP and DOWN the gradient can be fine adjusted Press lt gt the cursor keys to increase or decrase the temperature in increments of 0 1 C To go back to the spreadsheet press Soft Key Table 5 3 11 Program linear gradient Press softkey Edit linear gradient User BS 11 03 10 Edit program BS 01 ted 28 07 09 Edit linear gradient in step 27 1 Annealingtemp Eo 6 Increm C DC 00 0 0 Bud 00 ERN EE 0 0
21. The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program Do not heat samples without having the lid locked securely Be aware that samples are reaching high temperatures Do not touch or open hot tubes or microplates because hot liquid may quickly spill 8 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company out Do not touch the heated lid Use only suited plastic ware in the TProfessional Basic thermocycler Tubes and plates must show good fit when placed in the thermoblock Only use tubes that are suited for high temperatures tight lids The TProfessional Basic Thermocycler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Do not use the instrument when damages of the housing block cable or other parts are visible Prior to connecting the unit to the power source please ensure that the voltage selector at the bottom of the instrument is set to the required voltage Make sure that the main supply voltage is in accordance with the label above the power connection see section 4 2 Unplug the power cable before you open
22. ack the instrument 6 6 4 Set boot language The language displayed during the boot process can be set by the supervisor 6 6 5 Delete all user accounts The supervisor can delete all user accounts and including all progams In the next screen confirm with OK to delete all accounts Note Deleted user accounts and programs can not be reconstituted 56 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 7 Short manual Safety Warnings Do not open the instrument unless you are authorised to do so Check the label at the backside if the instrument has correct voltage configuration 110V 115 V or 230 V The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes If you still decide to use oil do not use silicon oil Mineral oil may be used Ensure that both the rear and bottom ventilation slits at the rear and bottom of the instrument are unobstructed Insufficient ventilation can cause overheating of the instrument The TProfessional Basic Control Panel Menu Buttons Bl
23. and confirm with Soft Key Save Contrast Instruction Manual TProfessional Basic 06 2011 49 6 2 3 Set time and date Biometra An Analytik Jena Company 12 01 06 2 3 Set date and time Date Day Month 1 Year 2006 Hours 15 Minutes 10 and Time Enter settings for Date and Time and confirm with Soft Key Set Time and Date 6 2 4 Defragment memory By erasing and creating PCR protocols after a while new files may be stored fragmented in the memory of the TProfessional Basic Thermocycler This leads to increased memory access times and the software reacts slower to user inputs By defragmentation the information becomes sorted and is stored in order thus leading to decreased memory access times Subsequently the software reacts faster to user inputs 08 07 09 15 06 2 4 Defragment memory 6 2 5 Screen saver 50 To defragment memory press DE This may take several minutes Overview Confirm your selection with OK and wait until the defragmentation process is finished Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company Screen saver P wr starts after 30 min 30 60 min Overview Set the screen saver ON or OFF and if necessary change the time after that the screen saver is started Standard time is 30 min Press Save to save the changings 6 3 System info This Menu provides information on the instrument and on the Log files of the
24. b Deutschlands wenden Sie sich bitte an den lokalen H ndler Dieses Symbol gilt nur in Staaten des EWR EWR Europ ischer Wirtschaftsraum umfasst die EU Mitgliedsstaaten sowie Norwegen Island und Liechtenstein Cet symbol conteneur d chets barr d une croix signifie que le produit en fin de vie doit tre retourn un des syst mes de collecte mis la disposition des utilisateurs finaux en cons quence des r gulations par la loi de votre pays Pour des information additionel nous Vous demandons de contacter votre distributeur Cet symbole s applique uniquement aux pays de I EEE EEE Espace conomique europ en qui regroupe les tats membres de l UE et la Norv ge Islande et le Liechtenstein TO Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 14 Declaration of Conformity EU Konformit tserkl rung EC Declaration of Conformity G ttingen April 2011 im Sinne der EG Richtlinie ber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 2006 95 EG following the EC directive about electrical equipment for use within certain limits of voltage 2006 95 EC und and im Sinne der EG Richtlinie f r die elektromagnetische Vertr glichkeit 2004 108 EG following the EC directive about the electromagnetic compatibility 2004 108 EC Hiermit erkl ren wir dass folgende Thermocycler Herewith we declare that the following Thermocyclers Typen
25. cal details for the Biometra Service Department 54 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 6 5 How to contact Biometra 12 01 06 How to contact Biometra Biometra GmbH Rudalt Wissell Str 30 37079 Goettingen Germany 49 09551 50686 0 49 0 551 50666 66 e mail infom biometa com www hbiametra carm 6 6 Supervisor system configuration The supervisor has access to an own system menu with some specific functions 6 6 1 Change user PIN The supervisor can change the PIN for each user account Select the appropiate user directory from the list and enter a new PIN 6 6 2 Delete user account Select the user directory to delete from the list and confirm with OK The directory and the programs therein are deleted 6 6 3 Change supervisor PIN Enter your actual PIN the default Supervisor PIN is 000 000 0 do not enter blanks enter your new PIN and repeat your input Confirm with PIN OK iT User SLIP 08 07 09 SUP Change SUP PIN Supervisor SUP attention Forgotten SUP PIN can only be changed by Biometra Service in Germany Enter current PIN pamm Enter new PIN Repeat PIN LNervieuw Instruction Manual TProfessional Basic 06 2011 55 Biometra An Analytik Jena Company Note Please make a note of the Supervisor Pin und keep it on a safe spot A forgotten Supervisor Pin can only be reset by Biometras Service Department and requires to send b
26. cellent technical specifications 1 2 Special features 1 2 1 TProfessional Basic Thermoblock The TProfessional Basic features a gold anodized 96 well block providing outstanding temperature uniformity The instrument is available in two different versions with 96 well or 96 well Gradient block 1 2 2 TProfessional Basic Lid The TProfessional Basic lid has been optimised to achieve two essential requirements prevention of condensation and reliable contact between samples and thermoblock The lid is powered by Biometras well know smart lid technology which automatically limits lid pressure to save tubes from damage In addition the new design of the High Performance Lid ensures an even temperature distribution between samples thus significantly improving temperature uniformity With one press on the front button the lid gently swings open and arrests in its end position 1 2 3 TProfessional Basic User interface The TProfessional Basic user interface incorporates Biometras proven spreadsheet programming philosophy Avoiding endless serial program windows all steps of a program are entered in a single screen This makes the creation of new or editing existing programs fast and easy One touch leads from the spreadsheet to an alternative graphical programming mode The TProfessional Basic manages up to 30 individual users all password protected and provides for a large program store 1 2 4 TProfessional Basic Housing The elegant TProfe
27. ck self test has not been initiated this recommendation window will appear again if the unit is switched on Instruction Manual TProfessional Basic 06 2011 53 Biometra An Analytik Jena Company It is recommended to execute an extended self test regularly 6 4 3 View log files from extended self test The results of the extended self test logfiles are stored by the instrument To view a summary of the self test select the suitable logfile from the menu User FRA 08 07 09 4 3 Select logfile extended selftest Date Time Block type Serialna 12 09 05 09 09 Gradient 60 12345627 05 10 05 Gradient 60 12345067 06 10 05 Gradient 96 25780067 1 01 06 384 well 25685678 29 04 06 96 well 36543721 User FRA 43 View logfile of extended block selftest COver all performance block OK Serialno 12345627 Blocktype Gradient 60 Execution period 09 09 11 25 12 09 05 Check heating lid OK Check cooling element OK Check thermal tracking OK Check regulation OK Check gradient OK Check heating performance OK Check cooling performance OK In the next window the actual status of all important components is summarized at a glance 6 4 4 View Error log files A history of all software and hardware errors is shown in this menu Note Sever errors that require action by the User are displayed in the User Software Menu 6 4 5 Create Info file for Biometra Service Department This option creates a Service file containing techni
28. d time 2 To continue a paused program press Continue Continue Echoes EA ED oo EH To stop an active program press the Stop button on the left side of the keypad and confirm with OK in the TProfessional Basic program screen Programs with a pause in the last step have to be stopped manually as well Instruction Manual TProfessional Basic 06 2011 61 Biometra An Analytik Jena Company 8 Trouble shooting 8 1 Slow heating and cooling The TProfessional Basic is equipped with a strong ventilator for the cooling of the heat sink see also chapter 9 1 The inlet of this fan is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily from the inlet with a conventional vacuum cleaner 8 2 Autorestart Running programs will be continued from the same step where the power failure has happened as soon as the power is supplied again A message is displayed showing a that a power failure occurred during the run and b when and at which step the program was started again Auto restart 09 45 01 12 06 08 in step 1 706 1 1 Confirm with OK to close the message screen 8 3 Autorestart due to unrecognised power failure High voltage fluctuation can lead to an automatic restart of the thermocycler In this case the cycler restarts at the s
29. e Instruchons nenn onen ennnn nenne nenn anne nnn nnns 7 2 Safety and Warning Notices tt R 8 2 1 Definition of SYMDOIS nenn nnne nsn n nnns 8 2 2 General Safety Instructions nenn snnnnnnnennnnn 8 EE E Le Me A EE E EAE 11 3 1 Content TINN 11 3 2 UNDAaCK AH C HECK MNT TE M 11 3 3 JE CaO al COR ONE FIG ernten ee een 11 3 4 Operation voltage nnne anne nenn nnne nnnnennne nenne nano nenne sana senes 12 3 5 Initial self test power on self test 13 4 Operating demens nennen san 14 4 1 TProfessional Basic Thermocycler front view 14 4 2 TProfessional Basic Thermocycler rear view 14 4 3 The TProfessional Basic control panel ann nennen 15 4 4 High Performance Smart Lid HRS 15 S DENN 17 5 1 The TProfessional Basic User lntertace nennen nennen nennen 17 5 1 1 TProfessional Basic Menu Buttons 17 5 1 2 TProlessiohal Basi Soft Keys een 17 Srl Kolo Ag ea EEE EEE EEE 18 5 1 4 BOKEN 18 9 1 5 e U EE EE TO 19 5 1 6 P LOGAN Me ee ee 19 9 2 TProressional Basic SO BWAIG EE 20 5 2 1 Navigation within software Menus nenn 20 9 2 2 Mere NER c 20 0 2 3 Log in existing User 20 0 2 4 Create new user account 22 9 2 5 SUPPEN LOG EE 23 0 2 6 DEE USO need 23 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 5 3 ENN e E E EEAS 23 5 3 1 Create program from template eseessesssssseeeeeneenneene
30. e TProfessional Basic Thermocycler to room temperature before starting operation There must be sufficient distance between the ventilation slots on the rear side of the Thermocycler and a wall or another instrument min 10 cm Danger of overheating This instrument is designed and certified to meet EN 61010 1 safety standards It should not be modified or altered in any way Alteration of this instrument will void the warranty void the EN61010 1 certification and create a potential safety hazard Place the TProfessional Basic Thermocycler on a stable non flammable surface in a dry safe environment For details see working conditions in table Technical specifications see chapter 1 3 Do not use alcohol e g methanol ethanol organic solvents or abrasives to clean the instrument For transports always use the original Biometra box Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 3 Installation 3 1 Content of delivery 1 Thermocycler 2 Mains connector 3 Manual 4 Short Manual Please keep the original packaging material for return shipment in case of servicing The TProfessional Basic shipping box provides a specially developed system for contact free transport of this electronic device 3 2 Unpack and Check Unpack and carefully examine the instrument Report any damage to Biometra Do not attempt to operate this device if physical damage is present Please keep the origina
31. enn nenn nennen 62 9 Maintenance and repair EE 64 9 1 Cleaning and Mamtenance nenne nenn nennen nnne nnne nna nenn nennen 64 9 2 SANN 64 9 3 MMI TEN 64 9 4 Replacement Of Spare PariS aussen nenne 64 10 PRC COS SOS E 65 QUEEN elTe 65 Uu NME 010 E 66 11 1 Instructions for return shipment san nnnennnennne nennen nnnnnnnennne nenn 66 11 2 Packing of the Thermmocvcer nennen enne nnn nnns 67 12 Equipment Decontamination Certificate 2202002n00 n00 n0nanunnnunnnun eene nnns 68 13 Note for the disposal of electric electronic waste eee 70 14 Declaration of Conformity xrrnnernnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennnnnnnnennnnnnnnennnnnnnnennnnnnnnennnun 71 19 VV ALLAN EE 72 16 SUE NIX er 73 4 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 1 Introduction 1 1 Field of Applications The Biometra TProfessional Basic is a Thermocycler that is licensed for PCR applications research use only and intended to be used for performing polymerase chain reactions The Biometra TProfessional Basic Thermocycler is inspired by the wish to simplify molecular biology research To reach this goal the instrument housing lid and block were totally redesigned providing so far unrealized temperature uniformity Innovative user software was developed driven by Biometras 15 years experience in interface design The result is an easy to use Thermocycler with ex
32. ennnennennnn 24 5 3 2 Create program without template nenn nenn nennen 24 D 9 9 Set lid pre heating mode nnne nnns 25 5 3 4 DCE IG te EE d E 26 5 3 5 Enter step temperature and me 26 0 3 6 OOD m m 27 9 3 1 Cool below ambient temperature nennen nennen 28 5 3 8 FOO ral AUS RENTE TT T 28 5 3 9 SAVE DO AN EEE ee 28 MI BEST aA ETE 31 5 3 11 Program linear Gradient anne nnonnnnnn ann nenn ann nennen nnns 32 5 4 EINE 33 5 4 1 Insert program step EE 33 0 4 2 Delete program STE EE ee 35 5 4 3 elo NNN 36 0 4 4 Delete HON anne D m UU 37 5 4 5 Editing programs in the graphical mode 38 5 5 Change program optons nenn nenne san nnnnen 39 5 9 1 Heating and cooling BCE 40 0 9 2 Time increment gt Ate 40 9 0 9 Temperature increment or decrement gt ATC 41 5 6 UNN 41 5 6 1 Select program for start from User drechores 41 5 6 2 Quick start programs from the block Menu 42 5 6 3 View program prior to etart nenn 42 5 6 4 Display during operaton nennen 43 5 6 5 Pause Program QUANG IN seven 44 5 6 6 CONNUE ee 0 1 ag ee ee 45 9 1 AOP 0 9 i ee ene ee ET Tm 45 5 7 1 Stop Program from a pause nennen 45 5 8 Optimization of Fast PCR programs ann nnn nenn 45 6 SYstem SOUS RET 47 6 1 USER COMI ONAN ON NT Tm 47 6 1 1 i i A 0 0 gt ea UU 47 6 1 2 Change individual PIN E 4T 6 1 3 BEEN ACC OO e 48 6 2 System Copnfiguraton 48 6 2 1 GOFNQUFE2DEE EEE NE 48 6 2 2
33. equently 2 00 m s will be displayed in the 5 3 6 Set loop Note In general loops are entered in the LAST STEP of the loop A loop is defined by selecting the FIRST STEP in the loop plus the number of back loops Enter the number of the first step within the loop in the row go to and confirm with Enter Enter the number of back loops in the row loops Instruction Manual TProfessional Basic 06 201 1 27 Biometra An Analytik Jena Company ser FRA Edit program FRA 01 Block type Gradient 96 PreheatLid ON 99 C 2 5 Jmal 30 0 wa 4 foosof 50 0 00 30 2 24 Options The loop is shown as a bracket at the left side of the spreadsheet Note Total cycler number number of back loops 1 e g enter 24 for a total cycle number of 25 5 3 7 Cool below ambient temperature After the program has been finished samples can be kept at below ambient temperature ser FRA Edit program FRA 01 Block type Gradient 96 PreheatLid ON 99 C 35 forsto 300 sa 400 00 30 150 raue Note To save lifetime of Peltier elements a temperature of 15 C for the final step rather than 4 C is recommended 5 3 8 Program Pause To hold a temperature for an indefinite time enter 0 After confirming with Enter the word pause is shown 5 3 9 Save program Press button Save Save as 28 Instruction Manual TProfessional Basic 06 2011 User
34. g sheet move the cursor to the left side of the display Instruction Manual TProfessional Basic 06 201 1 39 Biometra An Analytik Jena Company er FRA 12 01 06 Edit program FRA 03 demo a 16 01 06 Block type Gradient 96 Preheat Lid OFF 99 C as ms 1 300 0 30 z00 c00EMEM 150 raue I Save AS If options have been changed from the default values there is an indication in the options field 5 5 1 Heating and cooling rate Since the TProfessional Basic is a fast thermocycler it may be necessary to reduce the heating and cooling ramp to adapt protocols from slower thermocyclers The average heating and cooling rates can be set in the column labelled with A C s User HD 11 09 09 16 40 Edit program HD O1 N Preheat Lid OM 99 C Block type crafient 96 ATCC At Insert Delete Mu Graph Save Step Save As Note This entry specifies the speed at which the referring step is achieved l e to heat from step 1 to step 2 the heating rate in step 2 has to be lowered Note To reduce the over all speed of the Thermocycler the heating rate has to be modified in all steps The speed settings will be only valid for this individual program 5 5 2 Time increment gt At s To compensate for loss in enzyme activity each step within a loop can be extended from cycle to cycle Enter the desired time increment seconds in the row labelled with dt s This value will be added to the time
35. gram FRA 03 demo a 15 01 06 Block type Gradient 96 PreheatLid OFF 99 C ar ea RER 300 os 150 Pause Biometra An Analytik Jena Company User FRA 12 01 06 Edit program FRA 03 demo2a 16 01 06 _ Block type Gradient 96 Grad 00 03 40 0 00 30 20 0 15 0 00 33 Pause oi az Joes 25x 4 as Save Save As To change temperature and time settings move cursor to the respective field and enter new values with the key pad Once confirmed with enter the temperature graph is updated Note The steps are automatically spread over the whole range to give maximum resolution thus steps height does not represent the real temperature Note To set loops go to the Spread Sheet Mode press Table Note To insert or delete program steps go to the Spread Sheet Mode press Table Note To edit or enter gradients move cursor to the respective step and press Soft Key Gradient Enter gradient temperatures as described in section 5 3 10 page 31 5 5 Change program options For each step additional options can be defined To change program options move the cursor to the right side of the spreadsheet labelled with Options A new table is displayed er FRA 12 01 06 12 22 Edit program FRA 03 demo a 15 01 06 Block type Gradient 96 Preheat Lid OFF 99 C ET T P 0 ni 50 0 ooo 2 24 150 Pause er FRA 12 01 06 BEE To go back to programmin
36. h the manual or parts of it in any form as copies micro film or other methods without a written authorisation from Biometra Biometra is pointing out that applied company and brand names are usually protected trade marks 1 4 3 Liability Biometra is not liable for damages and injuries caused by use not considering these operating instructions in parts or completely 1 4 4 Meaning of the Instructions Biometra recommends that you first read these instructions carefully This operation instruction is part of the product and should be kept over the full life time of the instrument It should also be forwarded to subsequent owners and users Make sure that additions and updates are inserted into the operation instructions Instruction Manual TProfessional Basic 06 201 1 T Biometra An Analytik Jena Company 2 Safety and Warning Notices 2 1 Definition of Symbols Definition Caution Refer to instruction manual Danger High voltage Fragile Danger Hot surface PbPPi 2 2 General Safety Instructions Please read this manual carefully before starting operation of the TProfessional Basic Thermocycler The TProfessional Basic Thermocycler is intended for sample incubation at varying temperatures e General safety precautions for laboratory work must be observed when working with the TProfessional Basic Thermocycler e he TProfessional Basic does not produce a sound power level that could be hazardous for the user
37. ilure Power consumption max 480 Watt 100 115 230 Volt 50 60 Hz eae 13 0 kg Working conditions 15 C to 35 C 70 air humidity max 2 000 NN Measured inside the block TProfessional Basic Gradient 6 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company 1 4 Legal Notes 1 4 1 PCR License Legal Disclaimer Purchase of a Biometra Thermocycler conveys a limited non transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of US Patents Nos 5 038 852 5 656 493 5 333 675 5 475 610 and 6 703 236 or corresponding claims in their non US counterparts owned by Applera Corporation No right is conveyed expressly by implication or by estoppel under any patent claim reagents kits or methods such as 5 nuclease methods or under any other apparatus or system claim including but not limited to US Patent No 6 814 934 and its non US counterparts which describe and claim thermal cyclers capable of real time detection Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Biometra biomedizinische Analytik GmbH Rudolf Wissell Str 30 370779 Goettingen Germany Kmec and auikorizd 1 4 2 Copyright All rights reserved It is not allowed to copy and publis
38. is in accordance with the label above the power connection see section 4 2 e Connect the TProfessional Basic Thermocycler to a grounded socket Prior to connecting the unit to the power source please ensure that the voltage selector at the back side of the instrument is set to the required voltage Danger of electric shock Unplug the power cable before you open the TProfessional Basic Thermocycler e The display contrast can be adjusted to local lightning conditions 6 2 2 3 4 Operation Voltage Important Prior to connecting the TProfessional Basic to the mains make sure that the setting of the Voltage selector is in accordance with your mains Voltage The TProfessional Basic Thermocycler can operate at 100 115 or 230 Volt The operation Voltage is shown on the Voltage selector which is located at the instrument bottom To change operation Voltage of the TProfessional Basic switch off the instrument and disconnect the mains plug Use a coin or another round shape item to turn the adjustment slot of the Voltage selector to the new Voltage 12 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company Instrument bottom Instrument backside Voltage Mains plug indicator Adjustment slot Power switch 2 3 5 Initial self test power on self test After switching on the TProfessional Basic serial number of the instrument and the software version is displayed
39. ithin the range of 30 C to 99 C 2 Enter temperature C and time m s in the programming table for each single step To hold a temperature for an indefinite time enter 0 under m s and press enter Subsequently the word pause is shown in the display Note Minutes and seconds can be separated by dot You can also enter the digits one after the other Example to set 2 minutes 30 seconds enter 2 3 0 or 2 3 0 2 To insert or delete a program step move cursor to the corresponding position in the programming table and press Insert Delete Step R Gradient Graph Save Step Save As 2 To insert or delete a program step press Insert Step or Delete Step The position at which a step will be inserted or deleted is highlighted CANCEL Delete Insert Step Step 2 In the next screen confirm insertion or deletion with YES 2 To define a loop A enter the step the program shall go back to goto and B enter how often the loop is repeated loops The defined loop is graphically displayed by a bracket at the left side of the spreadsheet the total cycle number is mentioned 2 To program special features like temperature increment time increment or heating and cooling rate repeatedly press the cursor key right to open the options tab A new table is displayed Tip Storing samples at 16 C rather than 4 C at the end of a run will increase the instruments life
40. ive agents Important Appropriate safety regulations must be observed when working with infectious or pathogenic material 9 2 Servicing and repair The TProfessional Basic Thermocycler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal The Service department offers Thermocycler maintenances and temperature verifications Biometra recommends an annual maintenance and a biannual temperature check for all Thermocyclers Please call the following phone number for detailed information 49 551 50881 10 12 9 3 Firmware update For instruction for firmware upgrade please contact the Biometra Service Department or your local distributor sales representative 9 4 Replacement of Spare Parts Only original spare parts mentioned in these operating instructions are allowed 64 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company 10 Accessories 10 1 Plasticware Biometra offers a broad range of plasticware for the use in PCR The following table provides an overview for the product portfolio Cat no Description Quantity Single tubes 050 310 0 2 ml tubes with caps 1000 pcs 050 320 0 5 ml tubes with caps 1000 pcs Tube strips 050 254 Strips 8 tubes and flat caps 125 pcs 050 255 Strips 8 tubes and domed caps 125 pcs 48 well plates 050 225 48 well micro
41. l TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 6 4 1 View log files of Power on self test During boot up Power on the TProfessional Basic hard ware is checked Self test Here you can see the results of the last five self tests 6 4 2 Execute extended self test In addition to the regular Power on Self test a more comprehensive test can be triggered by the user In the extended block self test the unit can check the status and condition of different components and functions Therefore this test is an important and useful information to the customer whether the Thermocycler is still working within its parameters The extended block self test is listed in the System Menu under Service With Execute Selftest this test is initiated This test takes several minutes During the test no programs can be run on the thermocycler 12 01 06 47 Execute extended block selftest Check performance of block with extended selftest This may take several minutes You can t run programs during executian View result in 2 4 extended block selftest logfile In certain intervals the unit will automatically recommend to check the components of the unit i e to execute a new extended block self test message 729 Information Execution of extended block test is recommended 729 Quit Pressing Quit confirms that this recommendation has been noted In case the unit is switched off but the extended blo
42. l packing material for return shipment in case of service issues A I Attention A Please fill out and send back the warranty registration card This is important for you to claim full warranty 3 3 Installation Conditions e Place the TProfessional Basic Thermocycler on a stable surface in a dry safe environment For details see working conditions in table Technical specifications see chapter 1 3 e Let equilibrate the TProfessional Basic Thermocycler to room temperature before starting operation 1 to 6h e Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains e Make sure that the ventilation slots on the bottom and the rear are not obstructed see section 4 2 Make sure that there is no object underneath the thermocycler that may block the ventilation slots at the bottom e g a piece of paper etc e There must be sufficient distance between the ventilation slots on the rear side of the Thermocycler and a wall or another instrument min 10 cm Instruction Manual TProfessional Basic 06 2011 11 Biometra An Analytik Jena Company Ensure that both the rear and bottom ventilation slits of the rear and bottom of the instrument are unobstructed Insufficient ventilation can cause overheating of the instrument e Make sure that the main supply voltage
43. last five runs User FRA 3 System Info select topic or enter topic no Em System Info serial nu mbers software versions 32 Run Logfiles last 5 runs 6 3 1 System info Use this option to Display the instruments serial number block type and software version Instruction Manual TProfessional Basic 06 201 1 51 Biometra An Analytik Jena Company User FRA 12 01 06 3 1 View system info Cyclertype TProfessional Company Biametra Serial No Service Revisions No 0 0000001 Blocktype Gradient 96 Serial Nn 16741119 Software Yersion 0 25 0 30 0 7ztpr Protocol Yersion DO 0 0 1 0 2 1 0 0 2 1 0 MN very j pu 6 3 2 View Log files of the last five runs During each run a log file is stored in the Thermocycler memory The log files of the last five runs can be viewed in this window 6 4 Service 52 Liser FRA 3 2 Run logfile BBE 01 schering 1bc Log Time and Date 10 55 05 10 05 Start Endtime Date 08 07 09 05 05 09 05 06 08 03 User SME Halling Serial No 125 Cyclertype TPrafessional Company Biometra Software Yersion 0 38 0 29 2 5 1 Block 1 Block Serial No 3456 Blocktype Gradient 96 Messages Gradient 96 Program Messages ine aan User FRA 12 01 06 4 Service select topic 41 Poweron selftest logfile 47 Execute extended block selftest 43 Extended block selftest logfiles 4 Error Logfiles 45 Make Service Info Package Instruction Manua
44. ock LEDs on to loops Block 7 md 8 al 9 C m s Qo status SSG 300 jose 1 Run 2 300 00 30 u zs shao ejoa options Pause Ls soo fo 2 2 350 Pause 41 rn Program 1 Block Gradient Curve Save 4 Tyne Save As Stop Back d J J Ni l JAH Navigation Back Soft Keys Log in After the TProfessional Basic has passed the initial self diagnosis the login menu is displayed Press Log in and log in as existing user 2 Press Supervisor Log in and log in as supervisor Press New User and create new user account The language displayed in the user interface can be selected English or German The selected language will be stored for each individual user Instruction Manual TProfessional Basic 06 201 1 57 Biometra An Analytik Jena Company Log aut Select Language 2 Press Select Language to select preferred language Create modify and save programs 2 Tocreate a new or edit an existing program press the Program menu button a See ag weg wes geo N Move cursor to your directory and press Open directory ew Opern Program directory Press New Program or choose existing program and press Edit Program to enter the program screen NE we Prog
45. oils The Thermocycler is put onto the lower inlet and is fixed in between the foils by pressing the upper inlet down Note The Thermocycler is only protected from transport damage if the packing instructions are followed and the instrument is mounted in between the foils Biometra will not be responsible for transport damage by improper packing Folien schwebend Ger t zwischen den einspannen Mount instrument floating in between the foils 67 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 12 Equipment Decontamination Certificate To enable us to comply with german law i e 871 StrlSchV 817 GefStoffV and S19 ChemG and to avoid exposure to hazardous materials during handling or repair please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS PHONE NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Method of cleaning decontamination The equipment has been cleaned and decontaminated NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER 68 Instruction Manual TProfessional Basic 06
46. ook into consideration and adjust the elongation time corresponding to the length of the fragment to be amplified At a processivity of 35 bp sec the elongation time given in the protocol templates would be sufficient for the amplification of a 1000 bp long DNA fragment Note The proposed protocols are only applicable for the Thermocyler TProfessional Basic 96 To establish a Fast PCR at the Thermocyclers TProfessional 96 and TProfessional Standard 96 please follow the instructions in the homologue chapter of the corresponding manual 46 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 6 System settings To change global settings press the System Menu Button above the screen User FRA Topic list select topic or enter topic number 1 User Configuration change language change PIN delete user System Configuration beep contrast set date time System Info run logfile serial nurnber black info software versions Service selftest execute extended block selftest make Service Info Package error log How to contact Biometra Main screen 6 1 User configuration This option allows changes of your individual user account User FRA 12 01 06 1 System Configuration User select topic 11 Set language 12 Change PIN 13 Delete account To delete account empty user directory 6 1 1 Set language The language settings are part of each User account Available languages
47. plate 50 pcs 96 well plates 050 232 96 well skirted 25 pcs 050 213 96 well non skirted low profile 25 pcs 050 253 96 well non skirted 25 pcs 384 well plates 050 231 HSQ 384 well skirted 50 pcs 050 240 384 well microplate 50 pcs Miscellaneous 050 237 Silcone mat 50 pcs 050 256 Adhesive film 100 pcs 050 257 Heat Sealing Film 100 pcs 050 236 Heat Sealing Film Aluminium 10 pcs 050 194 96 well aluminium plate 1 pcs 050 694 384 well aluminium plate 1 pcs 050 251 Nop mat 96 well 20 pcs 050 252 Nop mat 384 well 20 pcs Instruction Manual TProfessional Basic 06 201 1 65 Biometra An Analytik Jena Company 11 Service Should you have any problems with this unit please contact our service department or your local Biometra dealer Biometra GmbH Service Department Rudolf Wissell Stra e 14 16 D 37079 G ttingen Phone 49 0 5 51 50 68 6 10 or 12 Fax 49 0 5 51 5068 6 11 e mail Service biometra com AN If you would like to send the unit back to us please read the following return instructions in chapter 11 1 11 1 Instructions for return shipment In case of an instrument failure that cannot be fixed by the procedures described in section 8 please proceed as follows e Return only defective devices For technical problems which are not definitively recognisable as device faults please contact the Technical Service Department at Biometra Tel 49 551 50881 10 12 Fax 49 551 50881 11 e mail se
48. ram gt Proaram gt Program Togra Use the cursor keys to navigate in the program screen In each case the activated field is displayed inverted Corresponding to the activated field the assignment of the Soft Keys in the TProfessional Basic control panel will change accordingly Note In the directory Biometra Shared you will find pre installed protocol templates for different PCR applications In chapter 5 3 1and 5 8 you will find instructions how to use these protocols The TProfessional Basic program screen Status display Status display Block type Preheat Lid User HL 2U U2 08 KR m s Defined cycles 3 6 0 eso o0 30 720 Joso 360 Pause A Programming table To alter block type activate the corresponding status display and press Block Type dene Graph Save Sten Save As Select appropriate block type from the menu in the next window The TProfessional Basic Thermocyclers automatically recognize the installed block module However it is also possible to write a program for an alternative block type 58 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 2 To switch lid preheating on or off activate the corresponding status display and press Preheat ON OFF Preheat EE Graph Save OM OFF Save AS Set lid temperature in status display under C w
49. re applied Release lid in down position 1 press pin 2 carefully turn wheel while holding the pin down COUNTER CLOCKWISE until you feel normal resistance no more clicking noise clutch is released Release pin and turn wheel counter clockwise until pressure is completely released Open lid Important When the clutch mechanism is active optimum pressure is applied do not use pin to further increase lid pressure This would lead to damage of tubes and instrument Instruction Manual TProfessional Basic 06 201 1 63 Biometra An Analytik Jena Company 9 Maintenance and repair 9 1 Cleaning and Maintenance The TProfessional Basic was built to operate for a long time without the need for periodical maintenance Nevertheless occasionally cleaning of the air inlet may be necessary to maintain the efficiency of the Thermocycler Insufficient airflow may lead to reduced heating and cooling rates see chapter 8 1 The inlet for the airflow is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily from the inlet with a conventional vacuum cleaner Additionally the Thermocycler housing may be cleaned from time to time with a smooth cotton cloth Do not use strong detergents or organic solvents for cleaning Never treat silver block with abras
50. ree I 05 free I 06 free By default the program is stored at the first free storage place available To save a program at a specific storage place use cursor keys up and down or enter two digits 2 To edit the name of the program press Edit name In the next screen occurring enter a name by using the keypad and confirm with OK Start pause and stop programs 2 The TProfessional Basic Thermocycler for each user stores the last 5 programs that have been run or modified For quick start of one of these programs press the Block menu button In the next screen select one of the last five programs by using the cursor keys up and down Press the Start button on the left side of the keypad to start the program 2 To start a program from a user directory log in with your user account and press the Program menu button In the next screen select the appropriate user directory and program by navigating with the cursor keys Press the Start button on the left side of the keypad to start the selected program After starting a program the following screen is shown 60 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company User HD 21 02 08 Program Block active germen User Hd HD 2 Block type Gradient 60 Remaining Time 2h 53m Estimated End 12 26 2 To pause an active pogram press Pause Block The message PAUSE is shown in the display and alternates with the elapse
51. rt Lid lower lid temperatures can be used for thermocycling than in the past Thanks to the lower lid temperature a higher temperature uniformity between samples can be achieved User FRA 12 01 06 Edit program FRA 01 Block type Gradient 96 Preheat Lid ON 99 C The recommended lid temperature is 99 0 C Enter temperature and confirm with Enter or step to the next field with cursor key 5 3 5 Enter step temperature and time c gt Use cursor keys to navigate in the programming table Note Each setting is confirmed with Enter The cursor moves automatically to the next field Alternatively you can confirm a value by moving forward with the cursor keys 26 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company er FRA 12 01 06 Edit program FRA 01 Block type Gradient 96 Preheat Lid ON 99 C ar Joel 300 oos gt 400 00 30 Enter temperature for the first step and press Enter In the next row you can enter the time for this temperature Note Minutes and seconds can be separated by dot but this is optional You can also enter the digits one after the other Example To set 2 minutes 30 seconds enter 2 3 0 Subsequently 2 30 m s will be displayed in the programming table Note For Biometra Thermocyclers a dot can be used as a shortcut to program minutes 5 amp 9 Example To set 2 minutes enter 2 programming table Subs
52. rvice biometra com e Please contact our service department for providing a return authorization number RAN This number has to be applied clearly visible to the outer box Returns without the RAN will be not be accepted e Important Carefully clean all parts of the instrument of biologically dangerous chemical or radioactive contaminants If an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device e Please prepare written confirmation that the device is free from biologically dangerous and radioactive contaminants The declaration of decontamination see section 12 must be attached to the outside of the packaging e Use the original packing material If not available contact Biometra or your local distributor e Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT e Please enclose a note which contains the following a Senders name and address b Name of a contact person for further inquiries with telephone number c Description of the fault which also reveals during which procedures the fault occurred if possible 66 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company 11 2 Packing of the Thermocycler Biometra uses an extra designed packaging system where the instrument is mounted in between two tearproof f
53. ssional Basic housing is designed for heavy use Due to an optimised air stream design even higher efficiency and better temperature uniformity is achieved The instrument works quietly and consumes little power which in turn leads to low heat emission The compact footprint saves valuable bench space Keyboard and display have been set to an angle that ensures reflection free viewing and ergonomic programming To facilitate servicing the TProfessional Basic housing can be completely removed from the chassis Instruction Manual TProfessional Basic 06 201 1 5 Biometra An Analytik Jena Company 1 3 Technical specifications Block Gold anodized 96 well block or anodized 96 well XL block Max Cooling Rate 2 5 C sec Avg Heating Rate 3 0 C sec Avg Cooling Rate 2 0 C sec Temperature Uniformity 55 C 0 20 C within 15 sec 70 C 0 30 C within 15 sec 95 C 0 60 C within 15 sec Block Temperature Range 3 C to 99 C Gradient Temperature Range 20 to 99 C Control Accuracy 0 1 C Block Formats 96 well 7Opl or 96 well XL 100ul Quick start of the last five programs view program prior to start Software options toggle between spreadsheet and graphical mode extended self test generate service files adjustable ramp rates view gradient temperature graph PC control over RS 232 Display Ya VGA screen 320 x 240 pixel Memory 350 typical programs in up to 30 user directories password protected fa
54. tem Menu e Block Menu e Program Menu The active menu is indicated by a graphical link to the respective menu button System Program Login GOE 05 09 05 The example shows Program Menu is active The four Menu Buttons are permanent and always have the same function This is in contrast to the Soft Keys The Soft Key functions change depending on the current software menu 5 1 2 TProfessional Basic Soft Keys Below the Display there are four SOFT KEYS Instruction Manual TProfessional Basic 06 201 1 17 Biometra An Analytik Jena Company In contrast to the Menu Buttons the Soft Keys have changing functionalities which are shown in the above display line 5 1 3 Log in Menu To enter the Log In menu press the Menu Button Log in out above the display When the instrument is switched on the Log in screen is displayed To use the instrument a user has to be selected Alternatively a new user can be created User FRA 12 12 02 To log in select user or enter initials Current user FRA Frank BAR Barbara EVA Eva FRA Frank JON Jonanthan eer Supervisor New User Log in Log in 9 1 4 Block Menu To enter the Block Menu press the Menu Button Block above the display User FRA 12 12 02 Block free Block type Unknown 18 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company The block menu shows the current status of the block The block can be acti
55. tep where there power failure has occurred A message is displayed showing a that a power failure occurred during the run and b when and at which step the program was started again see 8 2 To avoid voltage fluctuation do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge 8 4 Adaptation of protocols from other cyclers Since the TProfessional Basic is a fast instrument it may be necessary to reduce the heating and cooling ramps to run protocols from other cyclers For the setting of the heating and cooling ramps see section 5 5 1 Alternatively the time settings may be extended 8 5 Releasing wheel in case of blocked lid When the lid is in the very up or down position it may happen that the wheel is uncoupled In this situation the clutch mechanism is active in both directions clicking noise in either direction 62 Instruction Manual TProfessional Basic 06 2011 Biometra An Analytik Jena Company To unlock wheel press down metal pin with a ball pen and turn wheel carefully This pin overrides the automatic clutch mechanism Thus care must be taken not to apply excessive pressure Release lid in upper position 1 press pin 2 carefully turn wheel while holding the pin down CLOCKWISE until you feel normal resistance no more clicking noise clutch is released Release pin and turn lid down until the clutch mechanism is activated clicking noise optimum pressu
56. the cursor to the wanted location or directly enter a storage number two digits over the keypad Edit name and confirm with Soft Key Save Copy 5 4 4 Delete program Select a program from your user directory User FRA 2 01 06 Select program name or enter program number gt HE FRA Frank 4 Programs 01 demo 16 01 06 sl W ssen 03 demo23 16 01 06 Grad 04 demo3a 15 01 06 05 free 06 free Program Program Program Program Press Soft Key Delete program Instruction Manual TProfessional Basic 06 201 1 37 Biometra An Analytik Jena Company ser FRA 12 01 06 Select program name or enter program number Delete this program Confirm Deletion with Soft Key Yes User FRA 12 01 06 Select program name or enter program number gt HE FRA Frank 3 Programs I D2 free 03 demo23 16 01 06 Grad 04 demo3a 15 01 06 05 free 06 free Copy Delete New Edit Pragrarm Program Program Program This program store is now free 5 4 5 Editing programs in the graphical mode Programs are created in the spread sheet mode where new steps are easily defined by Temperature and Time In addition programs can be edited in the graphical mode which provides a schematic temperature plot of the different steps To enter this mode press Soft Key Graph Spread Sheet Mode Table Graphical Mode Graph 38 Instruction Manual TProfessional Basic 06 201 1 ar FRA Edit pro
57. the TProfessional Basic Thermocycler Danger of electric shock PEPPER PP Ss EE Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains Connect the TProfessional Basic Thermocycler to a grounded socket When only few samples are put in the block place additional tubes in the four corner positions This is to evenly distribute the lid pressure and prevents single tubes from excessive pressure Use of few tubes may result in damage of the tubes by excessive pressure Appropriate safety regulations must be observed when working with infectious pathogenic or radioactive material Ask the responsible local safety inspector for details The TProfessional Basic Thermocycler must not be used with explosive flammable or volatile liquids Do not place fingers between lid and housing when opening or closing the lid Before opening of the lid release lid pressure see section 4 4 It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes If you still decide to use oil do not use silicon oil Mineral oil may be used gt gt e eoe Instruction Manual TProfessional Basic 06 2011 9 Biometra An Analytik Jena Company Ensure that both the rear and bottom ventilation slits not clogged by dust or other material Danger of overheating Let equilibrate th
58. the display see chapter 5 3 1 to enter the TProfessional Basic program menu 24 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company User FRA 12 01 06 Select program name or enter program number gt HE FRA Frank CO Programs 02 free 03 free O4 free 05 free 06 free Program Program Press softkey Edit Program and in the next screen press New Program The programming table opens see chapter Fehler Verweisquelle konnte nicht gefunden werden 5 3 3 Set lid pre heating mode You can choose whether the lid is pre heated before the program starts This is to avoid evaporation during the initial heating phase Note During the preheating of the lid the block is held constant at 25 C Note After reaching the set lid temperature there is a 40 second equilibration phase before the block starts Note The default setting which is recommended for most applications is preheating On User FRA 12 01 06 Edit program FRA 01 Block type Gradient op Preheat Lid EST c However if the program should start while the lid is heating this can be set by pressing the Soft Key preheat on off After Lid settings have been made confirm with Enter to enter the programming spreadsheet Instruction Manual TProfessional Basic 06 201 1 25 Biometra An Analytik Jena Company 5 3 4 Set lid temperature Note Thanks to the design of the new High Performance Sma
59. tials Enter a personal identification number PIN consisting of 1 7 digits For security reasons you are requested to repeat the entry of your PIN Note PIN entry is optional If no password protection is wanted just confirm with User data OK However if no PIN is entered you account containing all of your programs can be modified by unauthorized personal Note Your PIN can be changed any time in the System menu see section 6 1 2 5 2 5 Supervisor Log in Only the Supervisor is authorized to change global instrument settings This includes update of firmware and the deletion of user accounts To log in as Supervisor press the Log in out Menu Button above the Display Press Soft Key Supervisor Log in and enter Supervisor PIN The default Supervisor PIN is 000 000 0 do not enter blanks 5 2 6 Delete User Once logged in you can delete your own account in the System Menu see section 13 1 3 In addition any user can be deleted by the Supervisor To delete a user log in as Supervisor see section 6 3 and select menu option delete user Choose the user account you would like to delete and confirm with OK Note By deleting a User Account all programs in the referring user directory will be deleted It is therefore recommended to safe the programs in a different directory prior to deleting a User account 5 3 Create program At Thermocyclers of the TProfessional Basic series programs can be created in two ways You
60. types TProfessional Basic TProfessional Basic Gradient TProfessional Basic XL TProfessional Basic XL Gradient Best Nr Order No 070 701 070 702 070 601 070 602 den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 2006 95 EG und der EC low voltage directive 2006 95 EC and the EG Richtlinie ber die elektromagnetische Vertr glichkeit 2004 108 EG entsprechen EC directive about the electromagnetic compatibility 2004 108 EC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN61326 EN61000 3 2 EN61000 3 3 EN61010 1 EN61010 2 010 Dr J rgen Otte Quality Manager Instruction Manual TProfessional Basic 06 201 1 71 Biometra An Analytik Jena Company 15 Warranty This Biometra instrument has been carefully build inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship as described under the following conditions This warranty is valid for 24 month from date of shipment to the customer from Biometra This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with the instrument This warranty is valid only if the instrument is operated as described in the manual
61. uction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 12 12 02 To log in select user or enter initials Barbara Eva Frank Jonanthan Log in Select User with cursor keys or enter first initial The cursor jumps to the first account starting with this letter 12 12 02 To log in select user or enter initials Barbara Eva Jananthan Press Soft Key log in and enter your PIN 12 12 02 Enter 7 digit PIN code for FRA Frank Remember to log out when this cvcler is no longer under your Supervision FIN code OK Instruction Manual TProfessional Basic 06 2011 21 Biometra An Analytik Jena Company Confirm PIN with button PIN code ok The current User is shown User FRA Welcome Current user To continue press one of the above menu buttons To proceed with editing or running a program select one of the Menu Buttons above the display 5 2 4 Create new user account Press Menu Button Log in out above the Display 12 12 02 To log in select user or enter initials BAR Barbara Eva Frank Jonanthan usn SE Log in To create a new User account press Soft Key New User 22 Instruction Manual TProfessional Basic 06 201 1 Biometra An Analytik Jena Company 12 12 02 Register new user Enter your name initials 1 15 letters 2 3 letters Enter PIN optional Repeat PIN 1 7 digits Iser Data OK Enter your name and 2 3 ini
62. ve with a program or free If the block is free not running the five most recently used programs by the active User are listed These programs can be started directly from the block menu select with cursor and push start Button 5 1 5 System Menu To enter the System Menu press the System Menu Button above the display User FRA 12 12 02 1 User Configuration change language change PIN delete user System Configuration beep contrast set date time System Info run logfile serial number black info software versions Service selftest execute extended block selftest make Service Info Package error log How to contact Biometra The System Menu allows global settings and provides information about the instrument The power on self test log files can be retrieved and service files generated a detailed description of this menu is shown in section 0 page 45 5 1 6 Program Menu To enter the Program Menu press the Program Button above the Display User FRA 12 12 02 Select directory 7 BAR Barbara TEVA Eva Jananthan Open directory Instruction Manual TProfessional Basic 06 2011 19 Biometra An Analytik Jena Company In the Program Menu existing programs can be edited or new programs can be created Each User has his own program directory where individual programs are saved Programs from other user directories can be used but not modified 5 2 TProfessional Basic Software 5 2 1 Na
63. vigation within software menus Four navigation keys provide easy navigation within the software screens The left and the right key have additional functionalities as described below The right cursor key moves the cursor to the next field This cursor can also be used to confirm data entry By pressing the right cursor settings will be saved and the cursor moves to the next field In the file directory this key moves to cursor forward to the next lower level The left cursor key moves the cursor back to the previous field In most screens this cursor is equivalent to the back key In the file directory this key moves the cursor back to the higher level 5 2 2 Login The TProfessional Basic manages up to 30 individual users For each user a directory is created where programs are stored Programs from other directories i e users can be read but not modified This allows to use all programs that are in the cycler memory but to write only in the own user directory Each User is identified by User Name plus Initials 2 3 letters Each User account can be protected by a PIN consisting of 1 7 digits If no PIN protection is wanted leave the field Enter PIN empty and confirm with Enter Note If no PIN protection is used programs in the User directory may be changed or deleted by unauthorized persons 5 2 3 Login existing User Press Menu Button Log in out above the Display A list of Users is shown 20 Instr

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