NOTE - Harvard Apparatus
Contents
1. RS 232 9 Pin D Sub Male Baud Rates 9600 19200 38400 57600 115200 Word Size 8 Parity None Stop Bits 2 RS 232 Specifications Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 46 PUMP TO PUMP CONNECTION RS 485 CAUTION DO NOT CONNECT TO FIREWIRE PORTS ON A PC DAMAGE MAY OCCUR TO PUMP AND OR PC Mating Face Pin RS 485 In Signal RS 485 Out Signal Notes 1 PWR IN PWR OUT 30VY Protected by resettable fuse 2 GND GND 100 Q wto GND 3 RS 485 A TIED TO PIN 4 termination 4 RS 485 B TIED TO PIN 3 5 TIED TO PIN 6 RS 485 A 6 TIED TO PIN 5 RS 485 B RS 485 In Out IEEE 1394 Sockets USB or RS 232 RS 485 RS 485 pig a 3 renee iy PUMP RS 485 J RS 485 PUMP RS 485 RS 485 PUMP a ADDR OUT IN out gt N ADDRn D Sub D Sub 9 2 IEEE BOER IEEE 1394 9 F F Cable 1394 Cable Cable Daisy chaining via RS 485 NOTE When daisy chaining with RS 485 at a minimum every 4th pump in the daisy chain must be self powered i e another stand alone unit This can be affected by force selection X NOTE When applying power to pumps in a daisy chain apply power to the last group in the chain first and work backwards X NOTE When removing power be aware that power removed from a stand alone unit will cause power to be removed from all RS 42. Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices Upgrading EZ PRO Software 1 Upload the latest software version to your desktop format is filename dfu 2 Connect the pump to PC using a USB cable 3 In Hyperterminal type Boot The pump s light should be flashing wy NOTE To exit bootloader mode without upgrading the software power cycle the pump NOTE First time users will need to install the PHD ULTRA USB driver as well as wy the bootloader driver file PHDUltra Bootloader inf Reference USB Virtual Comport Driver Installation 4 Disconnect then reconnect the USB cable From the CD provided with the pump open the PHD ULTRA Flash Upgrade Application Pump Flash Upgrade Application E x Select Pump To Upgrade File PHD Ultra 1 1 4 dfu Verify after download Optimize Upgrade duration Remove some FFs Upgrade Verity File correctly loaded 6 Check Verify after download then click browse Browse to the file previously saved on your desktop and click open 7 Click upgrade The previous software will first be erased the new software will be loaded and finally the software will be verified 8 Disconnect the USB cable and power cycle the pump Troubleshooting Infusion Accuracy To ensure infusion accuracy always use new syringe s and measure syringe bore diameter and enter actual dim
3. lt PHD_Ultra_Comr 2 Enter a name for a New Connection G e PHD_Ultra_Comm then click OK Connect To 3 Select the Virtual Comm Port from the Connect using drop down list Click OK df the Virtual Comm Port is not known use Device Manager to find it Instructions on setting up the Virtual CommPort Driver are supplied in Appendix E COM8 Properties 4 Set up the Port Settings as shown above and click OK Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 16 PHD_Ultra_Comm Properties 5 Verify the Settings are as shown above ASCII Setup mj M 6 Choose ASCII Setup and select Echo typed characters locally then click OK to complete the setup Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 17 PUMP CHAIN COMMANDS The Pump Chain commands allow all pump control information to be managed from an external computer source These commands can also be used to control a series of pumps up to 100 from a single computer interface PHD ULTRA PC Controlled Syringe Pump commands are communicated to the pump via the RS 232 or USB port interfaces through a terminal program such as the Harvard Apparatus Method Manager or HyperTerminal In using the Pump
4. SYRINGE LOADING 6 Release Tabs Pusher Block Syringe Holder Block Retaining Bracket Pusher Block Retaining Bracket Syringe Holder Block Syringe Barrel Clamp Knob Bracket Clamping Knobs Mechanical S Infuse Mechanical S Withdraw Pulley Cover Allen Wrench Holder Syringe Barrel Clamp Front view of PHD ULTRA PC Controlled Syringe Pump showing important controls for syringe loading Adjust the Pusher Block 2 to the approximate length of the syringe s by squeezing the Release Tabs 1 and sliding the Pusher Block 2 into position Loosen the Retaining Brackets 4 5 on both the Pusher Block 2 and Syringe Holder Block 3 by unscrewing the Bracket Clamping Knobs 7 8 Lift the Syringe Barrel Clamp 12 by unscrewing the Syringe Barrel Clamp Knob 6 Place the syringe s on the Syringe Holder Block 3 Ensure that the barrel flange is within the Syringe Holder Block s Retaining Bracket 5 and the plunger flange is within the Pusher Block s Retaining Bracket 4 Close up of syringe positioning with plunger inserted in retaining bracket of pusher block Lower the Syringe Barrel Clamp 12 by screwing down the Syringe Clamp Knob until the clamp is tight against the syringe barrel Push the Retaining Brackets 4 5 tightly against the syringe flanges then tighten the retaining brackets using the Bracket Clamping Knobs 7 8 Squeeze the Release Tabs 1 and move the Pusher Block 2 t
5. 4 4 4444 mm lt cr gt lt lf gt lt prompt gt gang Sets or displays the syringe count Quick Start mode only Command format gang syringe count Query response lt lf gt syringes lt cr gt lt lf gt lt prompt gt iramp Sets or displays the target infusion rate while ramping Quick Start mode only Command format iramp start rate start units end rate end units ramp time in seconds Query response lt lf gt Ramp not set up lt cr gt lt lf gt lt prompt gt or lt If gt Starting rate xl xxx lt cr gt lt If gt Ending rate xl xxx lt cr gt lt lf gt Ramp time seconds lt cr gt lt lf gt lt prompt gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 23 Using The PHD ULTRA PC Controlled Syringe Pump 24 irate Sets or displays the infusion rate Quick Start mode only Command format irate rate rate units max min Query response lt If gt xl xxx lt cr gt lt lf gt lt prompt gt wramp Sets or displays the target withdraw rate while ramping Quick Start mode only Command format wramp start rate end rate rate units ramp time in seconds Query response lt lf gt Ramp not set up lt cr gt lt lf gt lt prompt gt or lt If gt Starting rate xl xxx lt cr gt lt If gt Ending rate xl xxx lt cr gt lt lf gt Ramp time secon
6. 003 Flat Washer 2 5110 038 Dual Syringe Holder 2400 238 lamp i 2401 051 Knob Syringe ae Ch j p T Knob 2 5100 05 6 10 Multi Rack Syringe Holder Assembly Remove the four Set Screws from Syringe Holder using the provided hex key Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob Place the Multi Holder assembly on the Dual Syringe Holder and attach using four 10 32 x 12 Screws me SNS Attach Clamp Bar by screwing the Multi Syringe Clamp Knob into the threaded bar attached to the Multi Holder Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 35 Washer Flat 2 5110 038 Retaining Ring Shaft Brocket Clamp 5007 038 2401 023 Bracket AntiSyphon Thumbsorew 3 2401 025 6100 012 Ext Tooth Lockwasher 2 5174 003 usher Bar 2401 066 Knoo Bracket Clamp 2401 031 Ys o E Bracket Ant Syphon 2401 065 Washer Flat 3 5143 003 Bracket Hold Down 2401 048 Thumbscrew 2 5100 046 lat Head Screw 2 5137 015 6 10 Multi Rack Pusher Block Assembly 1 Remove Dual Syringe Anti Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft 2 Attach the Pusher Bar assembly to the Pusher Block using two flat head screws Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Ap
7. 8 ml min 215 8 ml min Display N A N A Non Volatile Memory Stores all settings Stores all settings Connectors RS 232 9 pin D Sub Connector 9 pin D Sub Connector RS 485 IEEE 1394 6 pos IEEE 1394 6 pos USB Type B Type B 1 0 amp TTL 15 pin D Sub Connector 15 pin D Sub Connector Linear Force Max 34 kg 75 Ibs 100 Force Selection 30 VDC Input 91 kg 200 Ibs 100 Force Selection 30 VDC Input Drive Motor 0 9 Stepper Motor Controlled 1 8 Stepper Motor Controlled Motor Drive Control Microprocessor with 1 16 microstepping Microprocessor with 1 16 microstepping Motor Micro Steps per one revolution of Lead Screw 12 800 6 400 Step Rate Minimum 27 5 sec ustep 27 5 sec ustep Maximum 26 usec ustep 52 usec ustep Pusher Travel Rate Minimum 0 18 um min 0 36 um min Maximum 190 80 mm min 190 80 mm min Power 12 30 VDC 50 W 12 30 VDC 50 W AC DC Adapter Power Supply GlobTek Inc P N TR 9CR1666LCP Y MED GlobTek Inc P N TR 9CR1666LCP Y MED Dimensions 11 75 x 5 5 x 6 5 in 29 8 x 14 0 x 16 5 cm 11 75 x 5 5 x 6 5 in 29 8 x 14 0 x 16 5 cm Weight 5 1 kg 11 2 Ibs 5 3 kg 11 7 Ibs Atmospheric Specifications Operating Temperature 4 C to 40 C 40 F to 104 F 4 C to 40 C 40 F to 104 F Storage Temp
8. Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Setting Up The PHD ULTRA PC Controlled Syringe Pump 10 PC TO PUMP CONNECTION OPTIONS Connect the PC Controlled Syringe pump to a PC using either an RS 232 or USB cable PC Controlled Pump PC control via an RS 232 or USB cable Pumps can be Daisy chained using RS 485 cables refer to Appendix E for more information RS 485 PUMP RS 485 RS 485 PUMP IN OuT IN ADDR n ADORI IEEE 1394 Cable Daisy chained pumps connected via RS 485 cables Multiple PC Controlled Syringe pumps can be controlled by a single PC by connecting through a USB Hub POWER STAND ALONE PC CONTROLLED PUMP ADDR 01 POWER STAND ALONE PC CONTROLLED PUMP ADDR 02 STAND ALONE PC CONTROLLED PUMP POWER ADDR 03 STAND ALONE PC CONTROLLED PUMP ADDR 04 ADDRESSES CAN BE 01 99 Setting Addresses on PC Controlled Units 1 Connect PC Controlled Syringe Pump unit to PC via an RS 232 or USB cable 2 Launch HyperTerminal 3 Set address using the appropriate Pump Chain commands Setting Forces on PC Controlled Units 1 Connect PC Controlled Syringe Pump unit to PC via an RS 232 or USB cable 2 Launch HyperTerminal 3 Set force to desired percent using the appropriate Pump Chain commands Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Setting Up The PHD ULTRA PC Controlled Syringe Pump
9. Programmable 70 3009 70 3309 N A N A Rack Options Catalog Description 70 3020 6 10 Multi Syringe Rack Holds up to 10 syringes from 0 5 ul to 20 ml and up to 6 syringes from 30 ml to 60 ml 70 3021 4x140 Multi Syringe Rack Holds four 100 ml glass or 140 ml plastic syringes only 70 3022 Microliter Multi Syringe Rack Independently holds 4 syringes from 0 5 ul to 10 ml 70 3023 Anti Siphon Kit Options Catalog Description 70 3036 PHD ULTRA Remote Satellite Internal Pinch Valve Option 70 3037 PHD ULTRA Remote Satellite Internal 3 way Isolation Valve Option 70 3034 PHD ULTRA Internal Fan Option 70 3035 PHD ULTRA Digital I O Option Only available with I W and Programmable Models Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Accessories Catalog No 70 4000 70 4021 70 4001 70 4020 70 4002 70 4003 70 4004 55 7760 72 2478 5113 001 70 4014 70 4015 70 4005 70 4006 70 2215 55 7002 55 7004 55 8000 5012 005 70 4013 70 4019 5005 070 Appendices 54 Description RS 485 Cable for Pump to Pump Communication 0 5 m RS 485 Cable for Pump to Pump Communication 1 m RS 485 Cable for Pump to Pump Communication 2 m RS 485 Cable for Pump to Pump Communication 9 m USB Cable for PC to Pump Communication 2 m USB Cable for PC to Pump Communication 5 m RS 232 Cable for PC to Pump Communication 9 pin D sub 2 m Cable Assy Daisy chain Legacy
10. RS 232 RJ 11 2 ft Cable Assy Daisy chain Legacy RS 232 RJ 11 7 ft Line Cord US 115 v Cable for Analog Control Option Adapter Analog Cable to Terminal Block Adapter PHD Digital I O Adapter D sub 15 to Term Blk Footswitch w Phono Plug Auto Fill Valve Box Normal Pressure 30 psi Auto Fill Valve Box High Pressure 200 psi Adapter for 25ml 50ml 100ml Hamilton GasTight syringes Hex Key 3 32 Lubricant SuperLube Icc Stand holds 1 master pump plus 2 satellites Power Supply AC DC Adapter 30VDC 50W For additional items such as Syringe Heaters Spill Sensors Temperature Controllers Nano Fluidic Accessories and much more please visit our website www harvardapparatus com or call 800 272 2775 Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Declaration of Conformity Manufacturer Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 U S A Phone 508 893 8999 We herewith declare that the following product Product Name PHD ULTRA PC Controlled Syringe Pump Series Model No PHD ULTRA Catalog 70 35xx To which this declaration relates is in conformity with the applicable EC Directives harmonized standards and other normative requirements Application of Council Directive s 2004 108 EC 2002 95 EC EU RoHs Directive Standard s to which conformity is declared Safety Exempt from LVD 2006 95 EC
11. RS 485 0 cccccecesceeeececeeeeeeeceeneeeeeneeeeseeeeeseneeeeneeeeens 46 Appendix F Maintenance serike iiri e EEE E TE EE R evap eebesth ted 47 Mai M ANCE seeren T a O ea 47 Battery Replacement cerieisa a E EEEE ESES 47 Upgrading EZ PRO Software ccc hugiisiichnu ane e a E EER EEEE 48 TroubleshootinE sste iir gout eveoetsesavessetenatnenner sass E ASTE tate AESA STE 48 Appendix G Valve Option siperi cna EE E E EEES EA EE 49 Appendix H Anto Fl Valves a eveeccssssrcecesccajeseseves an EEEE yan EERE E eas 50 Setting Up the Valve crisi e E E E E EEEE A EA 50 Appendix H Remote Stand se e occ cesendsncdccyvvvevuvay enen Sede soch ends EE EENE 51 Adjusting DIN R il Height cscri oiron isere R E E E E Eae Sinas 51 Adjusting DIN Rail Tilt csiscicccccesesessecisvsvbeetiessavienceesgeessteceoessetusvevsvsussevaevsasevecersaseestee 52 Mounntiag a PUMP svececescsscecssecccces ganas ausuatvevsneveseedecssivegecceseeessueuedeueustvevererseeteiveeeeeeess 52 DiSMOUNUNS a PUMP errea E 52 Appendix I Ordering Information s c 2 cccccisisvvissdeeeeeeeeeeceneacdcaeedeecsicasbevseveeeeeeveeeeveaaags 53 Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual General Information 4 General Information This guide describes basic procedures for operating the PHD ULTRA PC Controlled Syringe Pump including both hardware operations and software operations accessed through a PC This section discuss
12. Retaining Nut and screw the assembly back onto the pump Large vs small diameter syringe clamping Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Setting Up The PHD ULTRA 4400 PC Controlled Syringe Pump 13 PHD ULTRA 4400 SYRINGE LOADING Syringe Holder Pusher Block Retaining Bracket pusher block Retaining Bracket syringe holder nob leadscrew release 3 T knobs HARVARD syringe holder L N T knobs pusher block Syringe Clamp Thumb Screws View of PHD ULTRA 4400 PC Controlled Pump showing important controls for syringe loading 1 The Syringe Holder 1 and Pusher Block 2 are fitted with movable Retaining Brackets 3 4 which hold firmly the syringe barrel and plunger when refilling When loading the syringe into the pump it is necessary to adjust these brackets 2 Loosen the two T knobs 6 on the Syringe Holder and the two T knobs 7 on the Pusher Block to free the Retaining Brackets 3 4 3 To free the Pusher Block 2 from the leadscrew turn the Knob 5 on the front of the block until the knob slips into the slots 4 Place the syringe barrel on the Syringe Holder 1 and move the Pusher Block 2 to accommodate the plunger HARVARD View of syringe positioning with plunger inserted in retaining braket of pusher block Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User
13. be defective as to workmanship or materials This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original consumer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 using our toll free number 1 800 272 2775 or outside the U S call 508 893 8999 Email Address is techsupport harvardapparatus com Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges for non warranty repairs Please allow a reasonable period of time for completion of repairs or replacement If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may
14. mounting two satellite remote or PC controlled units with a Master Stand Alone pump at the stand s base DIN Rail E Satellite 2 Tilt Adjust Handles Satellite 1 Pump Rest Master Adjusting DIN Rail Height 1 Loosen both Height Adjust Knobs located on the sides of the Pump Rest 2 Slide the Pump Rest to your desired height 3 Tighten both Height Adjust Knobs securely NOTE If adjusting the height with a pump attached loosen one Height Adjust Knob then use one hand to support the pump while loosening the second Height Adjust Knob NOTE Slot covers provided as stops to prevent the Pump Rests from colliding are yw located in the slots on the side of the stand The stops can be trimmed to provide more traveling room for the Pump Rests To trim pop out the cover using a screw driver cut to desired length with scissors then snap back into place Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices DIN Rail perils mounting pring grooves Locking Spring Height Adjust Knob Tilt Adjust Handles Adjusting DIN Rail Tilt 1 Loosen both Tilt Adjust handles by turning counter clockwise To increae the handles range of motion pull the handle out and twist clockwise then allow the handle to click bback into place 2 Once both handles have loosened tilt the DIN rail to your desired angle and tighten into place using the Tilt Adjust h
15. parts are 316 stainless steel or Viton Syringes are available in 2 5 8 20 50 100 and 200 ml sizes with removable replacement tips The 20 to 200 ml stainless steel syringes offer SWAGELOK fittings in 1 16 1 8 and 1 4 inch sizes A luer lock fitting is also available The tips are interchangeable for 20 to 200 ml syringes The 2 5 ml is available with a 1 16 inch SWAGELOK tip only The 8 ml offers SWAGELOK fittings in 1 16 or 1 8 inch sizes All stainless steel syringes are constructed entirely of 316 stainless steel The 20 to 200 ml stainless steel syringes come standard with two Viton O rings for the barrel and one for the tip These Viton O ring seals insures against leakage These syringes are guaranteed to be leak free for pressures up to 750 psi The 2 5 ml stainless steel syringe contains one Perfluoroelastomer O ring seal on the tip and one Bal Seal on the barrel The 8 ml stainless steel syringe contains two Perfluoroelastomer O ring seals and two Teflon O Ring seals on the barrel and a Perfluoroelastomers O ring on the tip These syringes were designed to utilize the high forces available in our syringe pumps to produce pressures up to 7500 psi and 1500 psi respectively All syringes are supplied with inside diameter dimensions for use with Harvard microprocessor controlled pumps and rate charts for use with older classic pumps Replacement Viton O rings are available as are more chemically resistant Perfluoroelasto
16. selectable target volume or time values to control the total volume pumped for both the infuse and withdraw portions of a procedure PUMP CONFIGURATIONS The PHD ULTRA PC Controlled Syringe Pump is available in two configurations e Standard Pump This configuration is the most common It consists of a single unit with a dual syringe pumping mechanism attached to the top of the chassis e Push Pull Pump This configuration consists of a single unit with the syringe pumping mechanism attached to the top of the chassis The pump can hold up to four syringes 2 on either side of the pusher block The pump can simultaneously infuse and withdraw the exact same amount The PHD ULTRA 4400 PC Controlled Syringe Pump is available in one configuration e Standard Pump This configuration consists of a single unit with a single syringe pumping mechanism attached to the top of the chassis Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual SPECIFICATIONS Introduction To The PHD ULTRA PC Controlled Syringe Pump 8 PHD ULTRA PC Controlled Syringe Pump Specifications PHD ULTRA PC Controlled Pump PHD ULTRA 4400 PC Controlled Pump Accuracy 0 25 0 35 Reproducibility 0 05 0 05 Syringes Min Max 0 5 pl 140 ml 0 5 pl 140 ml Flow Rate Minimum 0 5 ul syringe 3 06 pl min 3 06 pl min Maximum 140 ml syringe 215
17. 1 Ext Tooth Lockwasher a 5174 003 Bracket Micro Anti syphon 2401 079 Microliter Multi Rack Pusher Block Assembly 5 Remove the Pusher Blocks Retaining Bracket by unscrewing the Bracket Clamping Knob completely and removing the Clamp Shaft 6 Attach Anti Syphon Bracket using the Clamp Shaft and Bracket Clamping Knob Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 40 Syringe Loading Microliter Multi Rack os Oe UR ae Release Tabs Pusher Block Retaining Bracket Pusher Block Bracket Clamping Knob Thumbscrew Cap Screw Cap Retaining Bracket Syringe Holder Syringe Holder T Knob Bumper Clamp Bar Assembly Front view of PHD ULTRA PC Controlled Pump showing controls for microliter multi rack syringe loading Squeeze Release Tabs 1 and adjust the Pusher Block 2 to the approximate length of the syringe s Loosen the Retaining Bracket 3 on the Pusher Block 2 by unscrewing the Bracket Clamping Knob 4 Loosen the Retaining Bracket 7 on the Syringe Holder 8 by unscrewing the two T Knobs 9 Create room for the syringe s by unscrewing the Screw Caps 6 until the Bumpers 10 are at their maximum height Slide the syringe under the Bumper 10 placing the barrel flange within the Syringe Holder s Retaining Bracket 7 and the plunger flange within the Pusher Block s Retaining Bracket 3 NOTE If yo
18. 10 Setting Address on PC Controlled Units ccc ccccccccce ce eeeeeeeeceeeeeeeseeeeeeaaaeeees 10 Setting Forces on PC Controlled Units s i i0cccceneeent csssisdieeecines cetcdss naeeedse 10 SVT BE Lodde s1 0 c c00ce bobs cine caaedeceddaavanananscasanacavdens S ESES EEEE N 11 Changing the bracket for small lt 30mD vs large gt 30ml syringes 0 12 PHD ULTRA 4400 Syringe Loading cece cceecceceeeeeeeeeeeceseeeeeeceseseeeeseeneneeeeeees 13 External Pump Control accriscia irre EEE 15 Setting Up HyperTerminal erensia a eliade 15 Pump Chain Command 50 2s0cccccededel cbetehesoteele bh EE aE TEE E Eiaa 17 Using the PHD ULTRA PC Controlled Syringe Pump Chain Commands 17 Eitorf MESSAGES ai cassie ds nis oe aS EEE che EE E E E EEE 18 System Command S csiis cccegececaseuthcnten uses va EINEN AAAA EEEN EEE 19 PUn COMMANS si a S 22 Rate Commands 2 0 csessvescaceccccescacacssacageneneveshievevwweee occcdeeecaasaacsudaevensnevesvewsOvewv ceeds 23 Volume COMM ANAS eiee anan a ceo eanantin nite Reese Bee E 25 Time Commands enren E sind a a TEE E 26 Digital T O Commands isoissa r a EE 27 Internal Command 5 ciieiess ceeds ceccwswanavavsceeenevavaegass sasaesdeceacasetuanananasanesauaseapnepueeyendedss 28 Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Table of Contents 3 Appendices saree E dnahcasaodcdesmetints 29 Appendix A Syringe Volume D
19. 2 Loosen the Retaining Bracket 7 on the Pusher Block using the Bracket Clamping Knob 5 3 Loosen the Retaining Bracket 6 on the Adjustable Syringe Holder 2 note that there are bracket clamping knobs 4 on both the front and rear of this assembly that need to be loosened 4 Extend Plunger on syringe s to maximum capacity Loosen Thumb Nut 8 and lift Syringe Clamp 3 Place the syringe on the Adjustable Syringe Holder 2 extend as necessary then lock into place by tightening the Adjustable Knob 1 Lock the syringe flanges into place by tightening the Retaining Brackets 6 7 Secure the syringe clamp 3 and tighten using the Thumb Nut 8 HARVARLE PHD ULTRA showing syringes installed on both sides of the Pusher Block NOTE For Syringes lt 10ml you will need to remove the Thumb Nut 8 and then remove wy and flip over the Syringe Clamp 8 Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 42 APPENDIX E EXTERNAL CONNECTIONS User I O Connector Specifications Pin Assignments 1 Direction control input Rising edge sets pump to infuse Falling edge sets pump to refill 2 Trigger Input Event trigger falling edge triggers a program event 3 Footswitch Input Momentary switch closure to ground or TTL Logic Low falling edge toggles between states run stop 4 Trigger 1 Output TIL Logic Output Default Low Control thru method o
20. 8 inch ID 1 4 inch OD silastic tubing and connector to syringe 55 7004 Autofill Valve Box High Pressure 200 psi Supplied with Swagelok fittings 70 4005 Adapter PHD Digital I O Setting Up the Valve Set up consists of three phases Voltage selection 110 or 220V Pump to valve electrical connection and Valve tubing to syringe hook up 1 Power Connection Connect the line cord to the power supply included Attach the power supply to the input jack on the valve box 2 Pump to Valve Electrical Connection The cable fitted with a male 9 pin D sub connector must be connected to the female D sub connector on the rear of the PHD ULTRA Syringe Pump using the Digital I O Adapter Cat No 70 3105 Power can now be switched on with the illuminated power On Off switch on the front valve housing 3 Pump to Valve Tubing Connection Swagelok fitting should be finger tight plus 1 1 4 turn Tubing is 304 stainless steel 1 4 inch O D wall thickness 0 035 inch r ra Silicon tubing 1 4 inch OD 1 8 inch ID Refill the SD we Syringe Pump to Valve Tubing Connection Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 51 APPENDIX I REMOTE STAND The Remote Stand accessory allows the user to maximize bench space by
21. 85 powered units deriving power from that stand alone unit X NOTE Power may be removed from any group of pumps except the first group without affecting communications downstream X NOTE Each pump in the daisy chain must have a unique address The first pump must be set to address 00 X Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 47 APPENDIX F MAINTENANCE Maintenance PHD ULTRA series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically every 100 hrs the pump will remind you The guide rods should be oiled using light weight machine oil and the lead screw should be lubricated with Super Lube Synthetic Grease provided with the pump To clean the exterior surfaces use a lint free cloth to remove loose dust For more efficient cleaning use a soft cloth dampened not soaked with water an aqueous solution of 75 isopropyl alcohol or a mild detergent Battery Replacement 1 Remove screw and swing cover to the side 2 Slide battery out from under clip 3 Install Lithium Coin Battery 3V 16mm CR1620 Harvard Part No 5155 288 or equivalent by sliding under clip with positive side facing out CAUTION USE ONLY SAME TYPE amp RATED BATTERY OBSERVE POLARITY WHEN INSTALLING
22. Chain commands you will need to assign each pump in the pump chain a unique address using the Address command in HyperTerminal The address range is from 00 to 99 This address value is used to identify which pump is to receive a command and which pump is responding The first pump in the chain the one connected to the computer must use address 0 zero Configure each pump with its assigned address and baud rate as described in the Pump Settings section of this Guide po NOTE Once communication is established if you manipulate the touchscreen you must reestablish communication lt CR gt NOTE System commands and start stop commands can be executed from method or B Quickstart screens For parameter commands it is necessary to run from Quickstart screen Using the PHD ULTRA PC Controlled Syringe Pump Chain Commands The following instructions will help you to utilize the Pump Chain commands feature on the PHD ULTRA PC Controlled Syringe Pump Commands may be abbreviated to the first four letters i e address would be abbreviated addr A space must follow the command if arguments are included If the pump address is nonzero the one or two digit pump address precedes the command For example to set the infuse rate for pump 12 the command would look like 12irat 3 2 u m In the command list below the following convention is used Required parameter Optional parameter Separator between parameter choices Numeri
23. Emissions Immunity EN 61326 2006 05 01 EN 61000 4 2 1995 A1 1998 A2 2001 EN 61000 4 3 2002 EN 61000 4 4 2004 EN 61000 4 5 1995 A1 2001 EN 61000 4 6 2007 EN 61000 4 8 1993 A1 2001 EN 61000 4 11 2004 EN 61000 3 2 2000 EN 61000 3 3 1995 A1 2001 CISPR11 2003 A1 2004 Group 1 Class A EMC compliance was evaluated by Intertek ETL Semko Reference test report file numbers 3196824 Box 005 the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date March 01 2010 C i Signature HARVARD a APPARATUS VP Engineering Operations Position Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual
24. HD ULTRA PC Controlled Syringe Pump Locking Spring for DIN Rail Mounting Allen wrench Groove for DIN Rail Mounting Rear view of the PHD ULTRA PC Controlled Syringe Pump showing important connections Half nut Pusher Release Knobs Block Guide Rods sad Screw Syringe Barrel Clamp Vertical Foot Syringe Holder Support Mechanical Stops Retaining Bracket Clamp Knobs User I O Connector 1 Footswitch S Input La Switch sold seperately Power RS 485 RS 232 USB Switch Connectors Serial Serial for pump to pump Input Input communication Front view of tbe PHD ULTRA PC Controlled Syringe Pump showing important components and controls POWER CONNECTIONS AND PUMP STARTUP CAUTION DO NOT CONNECT TO FIREWIRE DEVICES DAMAGE MAY OCCUR TO PUMP OR DEVICE The operating voltage range for the PHD ULTRA PC Controlled Syringe Pump is 12 30 VDC Only use provided 30 VDC 50W AC DC adapter power supply to guarantee specified operation and EMC compliance Use only the specified line cord and power supply for this product and make sure they are certified for country of use Plug the power cord into the Universal Power supply Plug the Universal Power supply into the power jack of the PHD ULTRA PC Controlled Syringe Pump Turn on main power switch located in the lower left corner of the front panel Pe UNS oa Power LED above the power switch will illuminate Publication 5419 006 Rev A
25. PHD ULTRA PC Controlled Pump Syringe Pump Series User s Guide PHD ULTRA Series MAL 70 35xx PATENTS PENDING HARVARD APPARATUS Publication 5419 006 Rev A Table of Contents 2 Table of Content General Information cscs ccc cece cm cesses es secs cencccetesncsseccceeescnccceseeeccinerecemnceee 4 Warranty and Repair Information 5 2 scssscccicetieetavisises sieves ccececcethedes cossevsesue yen seascisanecceeeee 5 Manual DESCHP THOM siccise erste cececesec te cvaycases R E E A ERE ESEESE E 5 WALTAIILY o r EEES E 5 Repair Facilities and Parts secsscisirecacrcriri tiit inniiid ieii Ae 5 Serii INUMPELS oieee E EEEE TEE E E EE EEA 5 CAD TAL OMS soree nE NTA aAA aE EEEE webbed instants 5 Safety Imformation Soenens a EE E E 6 Introduction To The PHD ULTRA PC Controlled PUMp ss ssssssseesseseeeeeneees 7 Product Overview Theory of Operation 2 0sssesves eccssedenecccetssceayseeneoenveevevenenaciess 7 Pomp Con POr ONS ein A ilai eee d TR 7 Specie mosean a E eE edovwextaevvtiuns chases 8 Setting Up The PHD ULTRA PC Controlled Pump ssessssssessesesessneeneneeneensnnes 9 Physical View es scocco sossscetecesa hs cedecx lt cvoisireeselsenteesgageescseepeatiaeteqcedieeuneeisevewsusneseeededaetes aes 9 Power Connections and Pump Startup 00 0 0 cece cece eee eeessnnneeaeeeeeeeeeeeeeeneesnenaaas 9 PGto Pump Connection OptlOns 2 2 28 ccdeenseeveveaesaeeias ee a Saa E seed ENV AERAR ETETETT sees
26. also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using a part number obtained from our Technical Support department If practical enclose a sample part or sketch We offer a complete reconditioning service Serial Numbers All inquiries concerning our product should refer to the serial number of the unit located on the rear panel Calibrations All electrical apparatus are calibrated at rated voltage and frequency While the flow and volume will stay calibrated the peak pressure may vary Harvard Apparatus recommends an annual calibration of the pump CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 5 General Information SAFETY INFORMATION Please read the following safety precautions to ensure proper use of your syringe pump If the equipment is used in a manner not specified the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Line Cord and Power Supply Use only the specified line cord and power supply for this product and make sure line cord is certified for country of use The operating voltage range for the PHD ULTRA PC Controlled Pump is 12 30 VDC The universal power supply operating vol
27. andles NOTE Ensure that the DIN Rail is tightened securely using the Tilt Adjust handles to yw prevent any movement when the pump is attached Mounting a Pump 1 The rear side of a Satellite Remote pump has a groove with a locking spring for DIN rail mounting Position the pump s top groove and locking spring over the top edge of the DIN rail 2 Push the top of the pump down against the spring while pushing the bottom of the pump back until the bottom groove locks under the DIN rail Dismounting a Pump 1 Holding both sides of the pump push down on the top of the pump to depress the locking spring 2 Tilt the bottom of the pump out to relese the bottom groove from the DIN rail 3 Fift the pump off of the DIN rail Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 52 APPENDIX J ORDERING INFORMATION Standard Version Appendices 53 Description Stand Alone Remote Satellite PC Box System System PHD ULTRA Infusion Only 70 3005 70 3305 N A N A PHD ULTRA Infusion Withdrawal 70 3006 70 3306 70 3406 70 3506 PHD ULTRA Infusion Withdrawal Programmable 70 3007 70 3307 N A N A PHD ULTRA 4400 Infusion Withdrawal Programmable 70 3010 70 3310 70 3410 70 3510 Push Pull Versions Description Stand Alone Remote Satellite PC Box System System PHD ULTRA Push Pull Infusion Withdrawal 70 3008 70 3308 70 3408 70 3508 PHD ULTRA Infusion Withdrawal Push Pull
28. c value without preceding zeros HHEH Numeric value with preceding zeros HH A range of values lt cr gt Carriage return lt lf gt Line feed lt sp gt Space prefix Pump address prefix in the format if the pump address is not zero lt prompt gt Prompt see below Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 18 The following prompts are returned after a command is executed The pump is idle gt The pump is infusing lt The pump is withdrawing The pump stalled T The target was reached If the pump is in poll mode an XON character is added after the prompt If the pump address is nonzero the pump address is prefixed to the prompt without a colon In the following list of commands The pump address is prefixed to every response line followed by a colon e lt lf gt lt prompt gt is the response unless otherwise noted Error messages Error messages are displayed if the entered command cannot be executed for some reason The error message will take up two lines with the first line being the message type and the second line describing the error itself The second line may be up to 80 characters long Command errors Command errors are displayed when the command is unrecognized entered in the wrong mode or the state of the pump keeps the command from executing i e using the IRUN command if a limit s
29. d The third integer is the infused volume in femtoliters All three values are for the current direction The flag field consists of six flags e Flag one is the motor direction and will be T if the pump is infusing and w if the pump is withdrawing If the letter is lower case the pump motor is idle If upper case the pump motor is running e Flag two is the limit switch status If the infuse limit switch was hit I is displayed If the withdraw limit switch was hit W is displayed If no limit switch was hit or the pump does not have limit switches Is displayed e Flag three is the stall status and will be S if the pump has stalled Otherwise it will be e Flag four is the trigger input state and will be T if high and if low e Flag five is the direction port state and will be I for infuse and W for withdraw e Flag six is the target reached status It will be T if the target time or volume was reached and if not Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 28 Appendices Appendices APPENDIX A SYRINGE VOLUME DIAMETER REFERENCE TABLE Harvard Apparatus Cadence Science Inc Hoshi Stainless Steel SGE Scientific Formerly Popper amp Sons Size Diameter Size Diameter Glass Engineering MICRO MATE Glass DRES e f 1 ml 6 50 mm 2 5ml 4 851 mm Size Diameter Size Diam
30. ds lt cr gt lt lf gt lt prompt gt wrate Sets or displays the withdraw rate Quick Start mode only Command format wrate rate rate units max min Query response lt If gt xl xxx lt cr gt lt lf gt lt prompt gt NOTE For commands requiring rate units use the following format m u n p h m S BA ex m m milliliter minute n s nanoliter second Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 25 Volume commands civolume Clears the infused volume Quick Start mode only Command format civolume ctvolume Clears the target volume Quick Start mode only Command format ctvolume cvolume Clears both the infused and withdrawn volumes Quick Start mode only Command format cvolume cwvolume Clears the withdrawn volume Quick Start mode only Command format cwvolume ivolume Displays the infused volume Quick Start mode only Command format ivolume Query response lt If gt xl lt cr gt lt lf gt lt prompt gt svolume Sets or displays the syringe volume Quick Start mode only Command format svolume tvolume Sets or displays the target volume Quick Start mode only Command format tvolume target volume volume units Query response lt If gt Target volume not set lt cr gt lt lf gt lt prompt gt or lt lf gt xl xxx lt cr gt lt
31. e syringes are loaded it may be difficult to manually move the p Pusher Block in order to set the Mechanical Stops Instead use a single empty syringe to measure the proper lengths and set both the Infuse and Withdraw Mechanical Stops first Then use the above procedure to load your syringes Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 38 Mounting a Microliter Multi Rack Clamp Bar Assembly 2400 290 Spacer Micro Clamp 2 2015 045 p 2 T Knob 2 5100 05 Spring Washer 2 Micro Holder 1 10 00 2401 080 Micro Retainer 2401 081 Bracket Syringe Clamp 2401 050 Dual Syringe Holder ii 5401 011 Microliter Multi Rack Syringe Holder Assembly Remove the four Set Screws from Syringe Holder using provided hex key Remove Syringe Clamp and Knob from the Syringe Holder First assemble the Retaining Bracket to the Micro Holder using the two T Knobs Place the assembled Micro Holder onto the syringe holder and screw in the two Standoff Bars yii Pio a Place the Clamp Bar Assembly onto the Micro Holder by screwing the Thumbscrew Caps into the Standoff Bars Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 39 Retaining Ring 5007 038 Shaft Bracket Clamp 2401 028 Bracket Anti Syphon 2401 025 Washer Flat 2 5110 038 Knob Bracket Clamp 2401 03
32. ensions in millimeters mm using the Custom Syringe entry option Additionally make sure that the guide rods and lead screw are properly lubricated RS 232 Difficulties Verify that the baud rates data framing parameters data bits parity and stop bits on all devices are the same Verify flow control handshaking is set to None If an address is set to other than address make sure commands and queries are prefixed with the address Make sure straight through pinning is used for the cables do not use null modem cables Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 48 Appendices 49 Appendix G Valve Option ULTRA showing the internal 3 way isolation valve option catalog no 70 3037 Port size is 4 28 Three 1 16 barb fittings are included ULTRA PC Controlled unit showing the internal pinch valve option catalog no 70 3036 Loading tubing into an internal pinch valve While valve is not actuated load tubing into the inside slot Then using the valve on PC command actuate the valve Load the tubing into the outside slot Tube set is included Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 50 APPENDIX H AUTO FILL VALVES There are two Auto Fill Valve models available Catalog No Product 55 7002 Autofill Valve Box Normal Pressure 30 psi Supplied with 1
33. erature 10 C to 70 C 14 F to 158 F 10 C to 70 C 14 F to 158 F Humidity 20 to 80 RH non condensing 20 to 80 RH non condensing Mode of Operation Continuous Continuous Classification Pollution Degree Class 1 Class 1 Installation Category Supplier Name Harvard Apparatus Harvard Apparatus Supplier Address 84 October Hill Road Holliston MA 01746 84 October Hill Road Holliston MA 01746 Regulatory Certifications CE ETL UL CSA WEEE EU RoHS amp CB Scheme CE ETL UL CSA WEEE EU RoHS amp CB Scheme Safety Declarations EMC Declaration ANSI UL 61010 1 2004 Ed 2 Rev 2005 CAN CSA C22 2 No 61010 1 2004 Ed 2 IEC 61010 1 2001 Ed 2 Corrigendum 1 2002 Corrigendum 2 2003 CENELEC EN 61010 1 2001 CB Scheme CENELEC EN 61326 1 2006 ANSI UL 61010 1 2004 Ed 2 Rev 2005 CAN CSA C22 2 No 61010 1 2004 Ed 2 IEC 61010 1 2001 Ed 2 Corrigendum 1 2002 Corrigendum 2 2003 CENELEC EN 61010 1 2001 CB Scheme CENELEC EN 61326 1 2006 Fan option is required if external operating ambient is expected to be gt 35 C Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Setting Up The PHD ULTRA PC Controlled Syringe Pump 9 Setting Up The PHD ULTRA PC Controlled Syringe Pump PHYSICAL VIEWS The following diagrams show the important components of the P
34. es important conventions used in this guide Illustrations Unless otherwise indicated the values in the illustrations of this manual are examples only They are not intended to indicate the exact values you will see or to suggest the values you should use Special Messages Callouts The following special messages and callouts appear throughout the guide to indicate information that requires special attention Warnings or hazard instructions provide information to help you avoid personal injury or damage to the PHD ULTRA PC Controlled Pump during operation ym Notes provide helpful instructions that can help you make better use of B the PHD ULTRA PC Controlled Syringe Pump Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual General Information WARRANTY AND REPAIR INFORMATION PHD ULTRA PC CONTROLLED SYRINGE PUMP BEFORE PLUGGING f CAUTION REFER TO SAFETY INFORMATION AND SETTING UP THE IN PHD ULTRA PC CONTROLLED SYRINGE PUMP Manual Description This manual is designed to provide all operational and program information required to operate and maintain the PHD ULTRA PC Controlled series pumps The functions and features are described in the Technical Specifications section Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to
35. eter 2 9 10 8 9 525 5 pl 0 343 mm 0 25m 3 47mm 3 10 00 20 19 13 10 0 485 0 5 3 62 5 12 60 50 28 6 25 0 728 1 4 82 10 1510 100 34 9 50 1 03 2 8 91 20 20 45 100 1 457 3 8 91 30 22 50 Air Tite 250 2 303 5 11 71 50 25 60 HSW Norm Ject 500 3 257 10 14 65 100 34 00 i 20 19 56 Size Diameter Ta a mm 30 227 Natsume 25 g6 5 10301 2 ge Sze Diameter 10 14 567 5 12 45 25 23 0 25m 2 5 mm 10 15 9 50 27 5 Becton Dickinson 0 50 2 8 20 20 05 100 35 Glass All Types 1 4 0 30 22 9 i 2 6 2 50 29 2 Size Diameter 3 7 4 l suai tie 0 5 ml 464mm 5 9 2 Becton Dickinson oneal ypes 1 4 64 Plasti pak Size Diameter 2 5 8 66 Top Size Diameter 0 5 pl 0 103 mm n o Size Diameter 1 ml 4 699 mm Hee 20 19 13 1 ml 6 40 mm 3 8 585 5 0 343 30 22 7 2 5 9 30 5 11 989 10 0 485 50 28 6 5 13 10 10 14 427 25 0 729 100 34 9 10 15 3 20 19 05 50 1 03 20 21 0 30 21 59 100 iae Sherwood Monoject 30 23 0 50 26 594 350 2304 Plastic 50 29 0 ef 26 994 500 3 256 Size Diameter 1 l 608 mm P 4 674 MERO Teramo 1 25 5151 3 8 865 Size Diameter ize Diameter 2 5 7 285 6 12 600 1 ml short 6 6mm 1 ml 4 70 mm 5 10 3 12 15 621 1 mllong 4 7 3 8 95 mm 10 14 567 20 20 142 2 5 ml 9 0 5 13 25 23 033 35 23 571 5 13 0 10 15 8 50 32 573 60 26 568 10 15 8 20 20 15 100 32 573 140 37 948 20 20 1 30 23 1 30 23 2 60 29 1 50 29 1 Suggested Force Level Settings for Common Syringes Syringe Type Material Capacity Force Setting Common Man
36. following section details the installation of the Virtual CommPort Driver supplied with the PHD Ultra pump Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Wi b site with your permission yp Can Windows connect to Windows Update to search for software Yes this time only Yes now and every time cornect a device No not this time Click Next to continue lt Back Next gt Cancel 1 On connection of the pump to the computer via USB the Found New Hardware Wizard will launch and ask how you wish to locate the driver Choose No not this time and click Next Found New Hardware Wizard This wizard helps you install software for Syringe Pump E If your hardware came with an installation CD QP or floppy disk insert it now What do you want the wizard to do O Install from a list or specific location Advanced Click Next to continue lt Back Next gt Cancel 2 Choose Install from a list or specific location Advanced and click Next Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices Found New Hardware Wizard Please choose your search and installation options C USER BHE Projects HarvApp SyringePumps P 3 Browse for the pr
37. format poll on off Query response lt If gt Polling mode is OFF lt cr gt lt lf gt lt prompt gt or lt lf gt Polling mode is ON lt cr gt lt lf gt lt prompt gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 21 time Sets or displays the date and time Command format time mm dd yy hh mm ss Query response lt I gt 4 4 HH M lt cr gt lt lf gt lt prompt gt Time format is 24 hours valve Displays or sets the valve state When the value is set to auto the motor direction determines the valve state Valve defaults to Auto on power up Command format valve on off auto Query response lt If gt Infuse lt cr gt lt lf gt lt prompt gt or lt lf gt Refill lt cr gt lt lf gt lt prompt gt vduty Sets or displays the valve duty cycle in percent Valid range is 5 to 80 Default value is 50 If a valve actuates but does not hold in the actuated position vduty command should be used to increase duty cycle percentage to allow valve to hold Command format vduty 5 80 Query response lt If gt lt cr gt lt lf gt lt prompt gt ver Displays the short version string Command format ver Query response lt lf gt PHD Ultra 4 4 lt cr gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Control
38. iameter Reference Table eeeeseeeerrrrrerrrrrreerre 29 Appendix B Stainless Steel Syringes 2 c sesisssesceseceseenccccccceccotedobabeceeeereseresveveveves ceeds 30 Appendix C Min Max Flow Rates senenin ie aan n a a S 31 PHD ULTRA 4400 Min Max Flow Rates ccccceecccceeeceeeeeereeeeneeceeeneeeseeneeesneeeeens 31 Appendix D Syringe Holder Options 2 0 0 0 ccc cece cece cece eeennnnnnnneeeeseeeeeeeeeeeeeeeeeees 32 Mounting a 4x140 Multi Rack cccccccccecccceceeseneceeceeesneeeeceseeneeeeeeeneneeeeeeesneeeeeeees 32 Mounting a 6 10 Mti RaCk oise isch do sated Nicaea 34 Syringe Loading 4x140 amp 6 10 Multi RackS 00 0 ccccccceecccceeseeceeeeeeeseseeessseeeeees 36 Mounting a Microliter Multi Rack occ cece cecceceeeeeeeeeeneneneneneeeeeeeeeeeeeeeeeeeeeeees 38 Syringe Loading Microliter Multi Rack ccccccceceeecceceeeeteeeeeceeeeeeeeeeeeeeeenneaaes 40 Syringe Loading Push Pull Mechanism 0 ccceeeeeeeeceeeeteceeeceeeeeeeeeeeeeeesnneaaes 41 Appendix E External Connections 2 0 0 0 ccceceeeecccccceceeceeeeeeeeeaaeaaaeaaaeeseeeeeeeeeeeeeeeeeeeees 42 User I O Connector Specifications 2 0 0 ccccccccceceeeeeeeeeeeeneneeeeeeseeeeseseceeeeeeeeeeees 42 USB essisiebcincielsieee niitisasie a Lili Rides E E E AE ETETE 43 USB Virtual Commport amp Driver Installation eee eeeeceeeeeeeeeeeeeeeesesnaaaes 43 RS 252 SPCCifiCAti ONS eiers na sca daabac seabed one E A oecaste 45 Pump To Pump Connection
39. led Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 22 lt lf gt lt prompt gt version Displays the full version string Command format version Query response lt i f gt Firmware v lt cr gt lt If gt RTOS FreeRTOS v http www freertos org lt cr gt lt lf gt Pump address lt cr gt lt lf gt lt prompt gt Run commands irun Runs the pump in the infuse direction Quick Start mode only Command format irun mun Runs the pump in the opposite direction Quick Start mode only Command format rrun stop stp Stops the pump Command formats stop stp wrun Runs the pump in the withdraw direction Quick Start mode only Command format wrun Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump Rate commands crate Displays the current rate that the motor is running at A valid response is returned only in dynamic situations while the pump is running Quick Start mode only Command format crate Query response lt If gt Infusing at xl xxx lt cr gt lt lf gt lt prompt gt or lt If gt Withdrawing at xl xxx lt cr gt lt lf gt lt prompt gt diameter Sets or displays the syringe diameter in mm Quick Start mode only Command format diameter syringe diameter Query response lt f gt
40. lf gt lt prompt gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump wvolume Displays the withdrawn volume Quick Start mode only Command format wvolume Query response lt If gt xl lt cr gt lt lf gt lt prompt gt Time commands citime Clears the infused time Quick Start mode only Command format citime ctime Clears both the infused and withdrawn times Quick Start mode only Command format ctime cttime Clears the target time Quick Start mode only Command format cttime cwtime Clears the withdrawn time Quick Start mode only Command format cwtime itime Displays the infused time Quick Start mode only Command format itime Query response lt lf gt seconds lt cr gt lt lf gt lt prompt gt or lt l gt 44 44 lt Cr gt lt lf gt lt prompt gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 26 Using The PHD ULTRA PC Controlled Syringe Pump ttime Sets or displays the target time Quick Start mode only Command format ttime target time Query response lt If gt Target time not set lt cr gt lt lf gt lt prompt gt or lt lf gt seconds lt cr gt lt lf gt lt prompt gt or lt If gt 44 44 lt Cr gt lt lf gt lt prompt gt wtime Dis
41. lt cr gt lt lf gt lt prompt gt NOTE If this command is entered via RS 232 the prompt is displayed at the previous B baud rate and then the baud rate is changed echo Sets or displays the RS 232 USB echo state Valid states are on or off Command format echo on off Query response lt lf gt Echo is OFF lt cr gt lt lf gt lt prompt gt or lt lf gt Echo is ON lt cr gt lt lf gt lt prompt gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 20 free Displays the number of free method steps left on the disk Command format free Query response lt lf gt steps used lt cr gt lt lf gt steps free lt cr gt lt lf gt total steps lt cr gt lt lf gt lt prompt gt The number of steps displayed is a four digit number right justified and padded with spaces force Sets or displays the infusion force level in percent Valid range is 1 to 100 Command format force 1 100 Query response lt If gt lt cr gt lt lf gt lt prompt gt metrics Displays the pump metrics The operation of this command is covered in the PHD Ultra Configuration document Command format metrics poll Sets or displays the polling mode state In polling mode prompts are inhibited if they aren t generated by a typed command Valid states are on or off Command
42. mer O rings High Pressure Stainless Steel Syringes With Luer Lock With SWAGLOK Diameter Syringe Size 1 16 inch 2 5 ml 70 2269 8 ml 70 2267 20 ml 70 2251 50 ml 70 2255 100 ml 70 2259 200 ml 70 2263 Replacement Tips F 2 5 ml 70 2246 8 ml 70 2246 20 ml to 200 ml 70 2247 urnished with Sealing O Ring Diameter Diameter 1 8 inch 1 4 inch N A N A N A 70 2268 N A N A 70 2252 70 2253 70 2254 70 2256 70 2257 70 2258 70 2260 70 2261 70 2262 70 2264 70 2265 70 2266 N A N A N A 70 2245 N A N A 70 2248 70 2249 70 2250 NOTE The PHD ULTRA PC Controlled Syringe Pump can accomodate syringes p ranging from 0 5 pl to 140 ml Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 31 APPENDIX C MIN MAX FLOW RATES Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on syringe manufacturer a E Minimum Rate Units geet 0 5 yl 0 103 mm 3 060 pl min 1 590 l min 1 pl 0 146 mm 6 120 pl min 3 181 pl min 2 pl 0 206 mm 12 240 pl min 6 359 l min 5 pl 0 343 mm 33 900 pl min 17 630 l min 10 pl 0 485 mm 67 860 pl min 35 250 l min 25 pl 0 729 mm 153 400 pl min 79 640 l min 50 pl 1 030 mm 306 200 pl min 159 000 l min 100 pl 1 457 mm 612 700 pl min 318 100 l min 250 pl 2 304 mm 1 532 nl min 795 500 l min 500 pl 3 256 mm 3 060 ni min 1 589 ml min 1000 pl 4 608 mm 6 128 nl min 3 182 ml min 1
43. ml 4 699 mm 6 373 nl min 3 309 ml min 2 5 ml 4 851 mm 6 792 nl min 3 526 ml min 3 ml 8 585 mm 21 270 ni min 11 040 ml min 5 ml 11 989 mm 41 480 nl min 21 540 ml min 8 ml 9 525 mm 26 180 nl min 13 600 ml min 10 ml 14 427 mm 60 070 nl min 31 191 ml min 20 ml 19 050 mm 104 700 ni min 54 380 ml min 30 ml 21 590 mm 134 500 nl min 69 850 ml min 50 ml 26 594 mm 204 100 ni min 106 000 ml min 100 ml 35 700 mm 367 839 nl min 182 500 ml min 140 ml 37 948 mm 415 600 ni min 215 800 ml min PHD ULTRA 4400 Pressure amp Force Specs Rate ml min Stall Force Lbs 10 gt 200 20 gt 200 30 gt 200 50 gt 200 60 gt 200 70 gt 200 80 gt 200 90 gt 200 100 gt 175 110 gt 175 120 gt 150 This is the rate indicated with a Harvard Apparatus 50 ml stainless steel syringe Diameter 28 6 mm cross sectional area 1 00 square inches Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 32 APPENDIX D SYRINGE HOLDER OPTIONS ya NOTE Syringe holder options are not available for 4400 mechanisms Mounting a 4x140 Multi Rack Clamp Bar 2401 071 Knob Multi Syringe Clamp 2401 076 10 32 x 1 2 Screw 4 5101 087 Multi Holder 2401 074 Multi Retainer 2401 070 Lock Washer 2 5174 003 1 Knob Singe lamp 2401 051 Flat Washer 2 5110 038 T Knob 2 5100 05 Dual Syringe Holder 2402 007 4x140 Multi Rack S
44. nt a balanced load 6 Push the Syringe Holder s Retaining Bracket 4 tight against the barrel flanges and tighten into place using the two T Knobs 8 7 Tighten the Anti Syphon Bar 5 using the three thumb screws If using the 6 x 10 rack flip the Plunger Holder 11 back into place and secure by tightening thumb screws 8 Lower or replace the Clamp Bar 7 and tighten thumb screw until Clamp Bar is firm against syringe s 9 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the right until the syringe plunger is located as far into the syringe as it will travel 10 Using the Allen Wrench located in its holder in the rear of the Pulley Cover 12 loosen the Infusion Mechanical Stop 9 and slide it to the left until it is in contact with the Pusher Block 2 Use the Allen Wrench to tighten it in this position Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 11 Appendices 37 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the plunger is located just to the left of the maximum volume allowable Use the Allen Wrench to loosen the refill Mechanical Stop 10 and slide it to the right until it is contact with the Pusher Block 2 Tighten the Withdraw Mechanical Stop 10 and then return the Allen Wrench to its holder 12 HARVARD 4 x 140 Multi Rack agonsedsssaiosostessnji oe 6 10 Multi Rack NOTE When multipl
45. nual Terminal N Pu NOTE 1 Satellite and Remote units are DC powered through the RS 485 cable Earth ground connection is through this cable for these units Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 6 Introduction To The PHD ULTRA PC Controlled Syringe Pump 7 Introduction To The PHD ULTRA PC Controlled Pump PRODUCT OVERVIEW THEORY OF OPERATION The PHD ULTRA PC Controlled Syringe Pump series is a family of high accuracy microliter and milliliter compatible pumps designed for a wide variety of applications including mass spectroscopy calibration drug and nutritional infusions microdialysis dispensing chromatography and LC HPLC The PHD ULTRA PC Controlled Syringe Pump incorporates a microprocessor controlled small step angle stepping motor that drives a lead screw and Pusher Block Advanced micro stepping techniques are employed to further reduce the step angle to eliminate flow pulsation The pump is engineered to provide flow accuracy within 0 25 and reproducibility within 0 05 The PHD ULTRA PC Controlled Syringe Pump requires control via a PC to control operation External I O interfaces permit external control via an independent computer or device The PHD ULTRA PC Controlled Syringe Pump is available in one model e Infusion Withdrawal This model supports infusion and withdrawal operations at user definable flow rates and with
46. o the right until the syringe plunger is located as far into the syringe as it will travel Using the Allen Wrench located in its holder in the rear of the Pulley Cover 11 loosen the Infusion Mechanical Stop 9 and slide it to the left until it is in contact with the Pusher Block 2 Use the Allen Wrench to tighten it in this position Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the plunger is located just to the left of the maximum volume allowable Use the Allen Wrench to loosen the refill Mechanical Stop 10 and slide it to the right until it is in contact with the Pusher Block 2 Tighten the Withdraw Mechanical Stop 10 and then return the Allen Wrench to its holder 11 Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 11 Setting Up The PHD ULTRA PC Controlled Syringe Pump 12 Mechanical Stop Mechanical Stop HARVARD Note mechanical stops set in position to prevent excess travel of pusher block PROPERLY TO PREVENT THE PLUNGER FROM BOTTOMING OUT ON j CAUTION BE SURE THE MECHANICAL STOPS ARE POSTIONED GLASS SYRINGES Changing the bracket for small lt 30ml vs large gt 30ml syringes 1 Remove the Syringe Barrel Clamp Assembly by completely unscrewing the Syringe Barrel Clamp Knob 2 Unscrew and remove the Retaining Nut and slide the clamp bar off the Syringe Barrel Clamp Knob 3 Flip the bracket over reassemble the
47. oper driver installation file and then click next It can be found on the CD supplied with the PHD ULTRA PC Controlled Syringe Pump Windows XP or Vista PHDUlItra XPVista inf Windows 2000 PHDUwUlItra w2k inf Hardware Installation The software you are installing for this hardware Syringe Pump XP Vista has not passed Windows Logo testing to verify its compatibility with Windows xP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway 4 Ifa warning message regarding Windows Logo testing is displayed choose Continue Anyway to proceed with the installation Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for J Syringe Pump XP Vista Click Finish to cose the wizard lt Back f i Cancel 5 The Virtual Commport software will be installed When the software installation is complete click Finish to close the wizard Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 44 Appendices 45 RS 232 Specifications Pin Signal 2 TXD 0900 3 RxD ODAO 5 GND 1 4 6 9 N C
48. pendices 36 Syringe Loading 4 x 140 amp 6 10 Multi Racks Release Tabs Pusher Block Syringe Holder Retaining Bracket Syringe Holder Anti Syphon Bar Pusher Bar Clamp Bar T Knobs Mechanical Stop Infuse Mechanical Stop Withdraw Plunger Holder Pulley Cover Allen Wrench Holder Front view of PHD ULTRA PC Controlled Pump with 6 10 Multi Rack showing controls for multi rack syringe loading NOTE Refer to Appendices for additional instructions on assembly of the Harvard Zz Apparatus Multi Rack Accessory 1 Raise or remove Clamp Bar 7 by unscrewing thumb screw 2 Loosen T Knobs 8 and slide the Retaining Bracket 4 on the Syringe Holder 3 open 3 Adjust the Pusher Block 2 to the approximate length of the syringe s by squeezing the Release Tabs 1 and sliding the Pusher Block into position 4 Loosen the three thumb screws on the Anti Syphon Bar 5 to provide enough room for the plunger flange of the syringe s If using the 6 x 10 rack loosen the two thumb screws on the outside of the Plunger Holder 11 Flip the plunger holder open 5 Place the syringe s on the Syringe Holder 3 ensuring that the barrel flanges are within the Syringe Holder s Retaining Bracket 4 and the plunger flanges are between the Pusher Bar 6 and Anti Syphon Bar 5 NOTE If using fewer syringes than the maximum number of syringes try to place them yw symmetrically about the lead screw to prese
49. plays the withdrawn time Quick Start mode only Command format wtime Query response lt lf gt seconds lt cr gt lt lf gt lt prompt gt or lt I gt 44 4 lt cr gt lt lf gt lt prompt gt Digital 1 0 commands input Reads and displays the trigger input port status Command format input Query response lt lf gt The trigger input is low lt cr gt lt lf gt lt prompt gt or lt lf gt The trigger input is high lt cr gt lt lf gt lt prompt gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 27 Using The PHD ULTRA PC Controlled Syringe Pump output Sets the level on one of the output ports Command format output 1 2 high low sync Sets the level on the sync port Command format sync high low Internal commands Internal commands are used by the pump when connected to a controlling computer but are not meant to be entered by the user They are listed here for completeness status Displays the raw status for use with a controlling computer Command format status The output is in three integer fields and one flag field all separated by spaces and terminated by a carriage return linefeed pair The first integer is the current rate in femtoliters per second The second integer is the infuse time in clock cycles One clock cycle is 1 60 000 000 second The time has a granularity of 1 millisecon
50. r serial comm 5 Trigger 2 Output TTL Logic Output Default Low Control thru method or serial comm 6 Sync Output TTL Logic Output Rising Edge Start Infuse Falling Edge Start Refill 7 Direction Output TTL Logic Output High Infuse Low Refill 8 Valve Output TTL Logic Output High Valve Actuated Low Valve Off Note Valve follows direction change when state is auto 9 13 Signal Return Ground 14 Run Indicator Voltage 5V through a 470 ohm resistor connect to LED Anode 15 Run Indicator Output TTL Logic Output active low dow run connect to LED Cathode Electrical Specifications Inputs Vy gt 2V Iry lt 20pA Vi lt 0 4V Ty lt 0 5mA Outputs Vog 23 8V Iop lt GMA VoL lt 0 4V IoL lt 6mA Publication 5419 006 Rev A All Inputs are pulled high to 5 VDC through a 10k resistor All Outputs are at TTL Logic Levels Trigger 1 Out Trigger 2 Out Footswitch In Sync Out Event In Direction Out Direction In Valve Out jo User I O Connector Specifications Run Indicator Run Indicator or Voltage Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 43 USB Mating Face Pin Signal 1 5V 2 Data 3 Data 4 GND USB Type B Female USB VIRTUAL COMMPORT DRIVER INSTALLATION When you connect the PHD Ultra pump to a computer via USB Windows will seek to install a driver for communication The
51. s Manual Setting Up The PHD ULTRA 4400 PC Controlled Syringe Pump 14 5 Make sure the syringe barrel flange and the plunger flange are held by the Retaining Brackets 3 4 Press the Retaining Brackets firmly against the flanges and tighten with the T knobs 6 7 6 Place the Syringe Clamp 8 over the syringe barrel and secure to the Syringe Holder with the two Thumbscrews 9 Close up side view of bracket positioning for small and large syringe sizes yw NOTE For larger syringes it may be necessary to flip the syringe clamp over Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 15 EXTERNAL PUMP CONTROL This section of the PHD ULTRA PC Controlled Syringe Pump manual describes the control of the pump using HyperTerminal on your PC National Instruments offers a LabView Driver that can be used with the PHD ULTRA PC Controlled pump To download this driver visit www ni com and search for Harvard Apparatus Setting Up HyperTerminal HyperTerminal is a Windows application designed to support the external control of devices such as the PHD ULTRA PC Controlled Syringe Pump through an RS 232 or USB connection The following instructions describe the configuration of the HyperTerminal application 1 Select Start All Programs Accessories Communications HyperTerminal Connection Description
52. tage range is 100 240 VAC 50 60 Hz Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded See Note 1 Make Proper Connections Make sure all connections are made properly and securely Any signal wire connections to the unit must be no longer than 3 meters except RS 485 pump to pump communication cable Observe All Terminal Ratings Review the operating manual to learn the ratings on all connections Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Avoid Pinch Hazard A pinch hazard may exist between the pusher block and end blocks Avoid placing fingers between these points while the pump is running Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qualified service personnel to perform inspection Orient the Equipment Properly Do not orient the equipment so that it is difficult to manage the connection and disconnection of devices Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Ma
53. u are using syringes larger than 1 ml it may be easier to unscrew one of the wy Thumbscrew Caps 5 and swing the Clamp Bar Assembly 11 to the side making more room to load the syringe s After the syringe s are in place swing the Clamp Bar back over the Syringe Holder 8 and reattach the Thumbscrew Cap 5 Lower the bumpers 10 until they are firm against the syringe s by screwing in the Screw Cap 6 Push the Syringe Holder s Retaining Bracket 7 tight against the barrel flange and tighten into place using the T knobs 9 Then push the Pusher Block s Retaining Bracket 3 tight against the plunger flange and tighten using the Bracket Clamping Knob 4 wy NOTE Mechanical stops are set using the same steps as a standard 2 syringe holder Microliter Multi Rack Configuration Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 41 Syringe Loading Push Pull Mechanism Adjustable Knob Syringe Holder Adjustable Syringe Holder Syringe Clamp Bracket Clamping Knobs Syringe Holder Bracket Clamping Knob Pusher Block Retaining Bracket Syringe Holder a t Retaining Bracket j 7 i Pusher Block v j Thumb Nut HA R VA R D Syringe Clamp PHD ULTRA showing Push Pull Mechanism installed To load syringes on the push pull pump 1 Loosen Adjustable Knob 1 to release the Adjustable Syringe Holder 2
54. ufacturer s Plastic Syringes lt 20ml 50 BD plastic Sherwood 520ml 100 Airtite Terumo Stainless Steel All 100 Harvard Apparatus Glass Glass lt 20ml 20 Cadence Popper gt 20ml 30 Glass Plastic lt 1000pl 30 Hamilton SGE lt 5ml 50 gt 5ml 100 B Publication 5419 006 Rev A NOTE The PHD ULTRA PC Controlled Syringe Pump can accomodate syringes ranging from 0 5 ul to 140 ml Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual 29 Appendices 30 APPENDIX B STAINLESS STEEL SYRINGES Cat No Product Replacement Parts 20 to 200 ml 5013 089 Chemraz O Ring 20 ml 5013 090 Chemraz O Ring 50 ml 5013 091 Chemraz O Ring 100 ml 5013 092 Chemraz O Ring 200 ml 5013 109 Chemraz Tip Seal O Ring sizes 20 200 ml 72 2472 Replacement Viton O Ring 20 ml pkg of 10 72 2473 Replacement Viton O Ring 50 ml pkg of 10 72 2474 Replacement Viton O Ring 100 ml pkg of 10 72 2475 Replacement Viton O Ring 200 ml pkg of 10 72 2616 Replacement Viton Tip Seal O Ring sizes 20 200 ml pkg of 20 72 2617 Stainless Steel Plunger Button to Adapt Syringe for Use with PHD 22 2000 HVP with Triple S Clamp Required for 50 ml and 100 ml syringes only Harvard High Pressure Stainless Steel Syringes Harvard offers a complete line 2 5 to 200 ml of Stainless Steel Syringes intended for high pressure applications with good resistance to most aggressive liquids Wetted
55. witch is active The command error has the following format lt lf gt Command error lt cr gt lt lf gt lt sp gt lt sp gt lt sp gt error message lt cr gt lt lf gt lt prompt gt Argument errors Argument errors are displayed when a command argument is unrecognized or out of range The argument in question will be displayed except in the case of missing arguments The argument error has the following format lt lf gt Argument error bad argument lt cr gt lt lf gt lt sp gt lt sp gt lt sp gt error message lt cr gt lt lf gt lt prompt gt Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Using The PHD ULTRA PC Controlled Syringe Pump 19 System commands address Sets or displays the pump address Valid range is 0 to 99 Command format address 0 99 Query response lt lf gt Pump address is lt cr gt lt lf gt lt prompt gt ym NOTE Pumps with an address of 0 are masters and pumps with an address between ZB 4 and 99 are slaves baud Sets or displays the RS 232 port baud rate Valid baud rates are 9600 19200 38400 57600 115200 128000 230400 256000 460800 and 921600 Note that some computers may not be able to handle baud rates above 115200 Command format baud 9600 19200 38400 57600 115200 128000 230400 256000 460800 921600 Query response lt lf gt baud
56. yringe Holder Assembly Remove the four Set Screws from Syringe Holder using the provided hex key Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob Place the Multi Holder assembly on the Dual Syringe Holder and attach using four 10 32 x 12 Screws Py Attach Clamp Bar by screwing the Multi Syringe Clamp Knob into the threaded bar attached to the Multi Holder Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 33 Ext Tooth Lockwasher 2 5174 003 Retaining Ring a 5007 038 Thumbscrew 3 5100 012 Shaft Bracket Clamping 2401 028 Bracket Anti Syphon 2401 025 Washer Flat 2 5110 038 Knob Bracket Clamping 2401 031 Pusher Bar 2401 067 Washer Flat 5143 003 Flat Head Screw 2 5137 015 4x140 Multi Rack Pusher Block Assembly 1 Remove Dual Syringe Anti Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft 2 Attach the Pusher Bar assembly to the Pusher Block using two flat head screws Publication 5419 006 Rev A Harvard Apparatus PHD ULTRA PC Controlled Syringe Pump Series User s Manual Appendices 34 Mounting a 6 10 Multi Rack Clamp Bar 2401 072 Knob Multi Syringe Clamp 2401 076 10 32 x 1 2 Screw 4 5101 087 Post Clamp Guide Multi Holder 2401 0 Multi Retainer 2401 075 C i 2401 050 Lock Washer 2 5174
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