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ProCartaMagnetic-Cell Culture.book

1. instrument Luminex or Luminex based MiraiBio Bio Rad or other Luminex instrument providers Required Equipment Material Source Part Number Hand Held Magnetic Plate Affymetrix QP0702 Washer Microplate shaker Labline 4625 or equivalent must have 3 mm orbit with ability to maintain 500 rpm Vortex mixer Major laboratory supplier MLS 100 mL capacity Corning Costar Adjustable single and multi MLS channel precision pipettes for dispensing 1 20 uL 20 200 uL and 200 1000 uL Reagent reservoirs 25 mL and Vist Labs 3054 1002 or equivalent CLS 4873 or equivalent Double distilled dd water MLS H O Microcentrifuge MLS Precautions and Technical Hints Thoroughly read this user manual and product insert that s included with the assay kit The product insert may contain specific instructions for proper use of the By Request or custom panels Before starting the assay turn on the Luminex machine and initiate the startup protocol It takes 30 min for the lasers to warm up Make sure the Luminex machine is calibrated according to the manufacturer s instructions When working with samples and standards change the pipette tips after every transfer and avoid creating bubbles when pipetting During the incubation steps cover the 96 well plate with aluminum foil to minimize exposure of the beads to light Be careful not to invert the Procarta 96 well Flat Bottom Plate during the assay or
2. Affymetrix Inc reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without notice Although this manual has been prepared with every precaution to ensure accuracy Affymetrix Inc assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information Copyright 2012 Affymetrix Inc All rights reserved Contents Contents iii Intended Use i a lala a aka anka lk al a ers kl a lk kk an ak salak al a ak al a l kk la Gi all d kk ak OO 1 Contacting Technical SUPPON ana dada LAND ka k a an kk a hala al ae ea 1 About Procarta Immunoassay Kits kk kK KK KK KK KII KK K K KI KIRI KI KIRI KI K KIRI K KI KI KI KI KIRI KK 1 HOW It WOKS oe eo a oboe bee ee oe Gee awa a d te ana di i na ERS ORD a 2 Procarta Immunoassay Kit Contents and Storage Conditions 000008 3 Required Equipment and Materials Not Supplied 00 00 00 ce ee 4 Precautions and Technical Hints kk Ak kk kk kK KK KK KK K KI eee 4 Sample Preparation u u au a a mn an K KIIV ENE KI KK KI KI KI KK KK RIK KIRI KK KI K KI K K KII KI KI eames 5 Preparing Antigen Standards zu 40 55 wkd dese oon KI KIRI K KI KIR K KIRI KI KI KIR KI KI KIR K KI KI K KI KI KK K 6 Performing the Assay kk kk kk kk KK KI KI KK KK KI nn KI KK KI KI K K KI an ey
3. Bring the 10X Wash Buffer to room temperature and vortex for 15 seconds Mix 20 mL of the 10X Wash Wash Buffer Buffer with 180 mL ddH20 NOTE 1X wash Buffer can be stored at 2 8 C for up to 6 months Bring the buffer to room temperature prior to use Step 2 Prepare the Mark the standard sample and blank wells For your convenience a blank layout is provided in the Plate Procarta 96 Well Flat Layout section Bottom Plate Step 3 Add the A Vortex the Antibody Magnetic Beads for 30 sec Antibody Magnetic B Add 50 uL of the Antibody Magnetic Beads to each well Beads C Insert the Procarta 96 Well Flat Bottom Plate into the Hand Held Magnetic Plate Washer so that the A1 location of the 96 Well Plate matches up with the A1 on the washer D Lock the 96 Well Plate in place by pushing the 2 securing tabs located on each end of the washer towards the 96 Well Plate until they overlap the skirt of the 96 Well Plate E Verify that the 96 Well Plate is securely locked by holding the assembly in the palm of your hand and gently pulling up on the 96 Well Plate F Wait 2 min to allow the Antibody Magnetic Beads to accumulate on the bottom of each well G Remove the liquid in the wells by quickly inverting the Hand Held Magnetic Plate Washer and 96 Well Plate assembly over a sink or waste container Do not remove the 96 Well Plate from the Hand Held Magnetic Plate Washer Blot the inverted assembly onto several layers of paper towels
4. allow contents from one well to mix with another well Ensure that the Hand Held Magnetic Plate Washer is securely locked into place prior to inverting when performing the wash steps Use a multi channel pipette and reagent reservoirs whenever possible to achieve optimal assay precision Store the detection antibody antibody beads Streptavidin PE samples and reconstituted standards including standard diluents sets on ice before adding to the 96 well plate For frozen samples thaw completely on ice and mix well prior to running the assay Sample Preparation Cell culture supernatant samples can be obtained from adherent and non adherent cells A total volume of 50 uL well of cell culture supernatant is needed and a minimum of 2 replicates is recommended Some samples may contain high concentrations of the analytes Dilution of the samples may be needed if the analyte concentration is above the assay upper limit of quantitation Serial dilution of the samples may need to be prepared to determine the appropriate dilution factor for accurately measuring the analytes of interest Use the same fresh culture medium used for culturing the experimental cells to prepare dilutions of the samples If the sample contains cells centrifuge the sample at 300 x g for 5 min at 2 8 C Collect the supernatant Use immediately or aliquot and store at 80 C The TGF B assay requires a special sample preparation procedure The TGF P assay can onl
5. ee woe 7 Setup of the Luminex Instrument ys lak a a a dk kla ee kw al al 9 AEI Pala RESURS AP ne x ab sr l WW huk a kb d l i lk A div W ene ked nk are 9 Troubleshooting kk kk kk kk kk KE KK KK KI KK KI KK KI KI KIRI KI KI KK KI KI KI KI KI KI KI KI KI KIRI KI KI KIR KK K 10 Validating the Hand Held Magnetic Plate Washer kk kK KK KI KI K KI KII KII KIRI K K KII KII 12 Example Plate 00 sess A a che 6 een AA 13 Blank Plate Layout kk kk KK KK KK KI KK KI KI KI KI KI KK KK K KI KI KI KI KI KI KI KI KIRI KI KI KI KI KI KI RIK KI KK KK 13 iv Procarta Immunossay User Manual Intended Use This user manual is for a Procarta Immunoassay Kit Magnetic Beads from Affymetrix to perform quantitative multiplexed immunoassays based on the Luminex technology The procedure is for simultaneous measurements of multiple protein biomarkers in cell culture supernatant samples The assay protocol and reagents supplied are not compatible with other manufacturer s reagents Each 96 well plate kit is configured to allow for the following usage 16 wells for an 8 point standard curve in duplicate 2 wells for blanks and up to 78 wells for samples Procarta Immunoassay kits can be stored for up to 6 months from the date of receipt when stored at recommended temperatures NOTE For the most current version of user documentation go to our website at www panomics com Contacting Technical
6. when performing washes IMPORTANT Do not substitute another plate type for the Procarta 96 Well Flat Bottom Plate Catalog No PC0169 included in the Procarta Immunoassay Kit This plate is specifically for use with the Hand Held Magnetic Plate Washer Catalog No QP0702 Other plate types can result in assay failure or poor assay performance After reading all instructions in this document we recommend that you perform a series of practice washes using the Hand Held Magnetic Plate Washer Step Action Step 1 Set Up Luminex Set up the Luminex instrument according to the guidelines provided Define a protocol with the Instrument appropriate bead regions and set to read 2 wells Refer to the Procarta Immunoassay Kit Product Insert for the target bead population of the panel Step 2 Prepare 96 Vortex Antibody Magnetic Beads Add 50 uL of the Antibody Magnetic Beads into each of 2 wells on Well Plate the 96 Well Flat Bottom Plate Step 3 Perform Wash A Perform a series of wash steps using the Wash Buffer to simulate the multiple wash steps in the assay No incubation needed Steps B After the final wash step add 120 uL Reading Buffer to each well Cover the 96 Well Flat Bottom Plate with a plate seal place on a shaking platform at room temperature and shake for 2 to 5 min to completely resuspend the Antibody Magnetic Beads Step 4 Read 96 Well A Insert the 96 Well Flat Bottom Plate into the instrument and read the 2 w
7. 00 rpm for 30 min at room temperature Step 9 Wash the 96 A Repeat Step 7 Well Plate Step 10 Add SAPE A Vortex the SAPE solution vial for 20 sec B Add 50 uL of SAPE solution into each well C Seal the 96 Well Plate with a new Plate Seal D Remove the 96 Well Plate from the Hand Held Magnetic Plate Washer E Wrap the 96 Well Plate with aluminum foil and shake at 500 rpm for 30 min at room temperature Step 11 Wash the 96 A Repeat Step 7 Well Plate Step Action Step 12 Prepare the 96 A Add 120 uL of the Reading Buffer into each well Well Plate for Analysis B seal the 96 Well Plate with a new Plate Seal wa nell C Remove the 96 Well Plate from the Hand Held Magnetic Plate Washer nstrumen D Wrap the 96 Well Plate with aluminum foil and shake at 500 rpm for 5 min at room temperature E Remove the Plate Seal prior to reading on the Luminex instrument NOTE We recommend reading the assayed samples on the Luminex instrument immediately However the 96 Well Plate can be wrapped with aluminum foil and stored for up to 48 hours at 4 C before proceeding After storage shake at 500 rpm for 5 min at room temperature prior to reading Delay in reading the assayed samples may result in decreased sensitivities for some assays Setup of the Luminex Instrument Sample Size DD Gate Timeout Bead Event Bead Region 100 uL 5 000 25 000 45 sec 50 100 If you are running assays on your Luminex i
8. KGS Panomics Atfymetrix Solutions User Manual Procarta Immunoassays Using Magnetic Beads For cell culture supernatant samples P N 17992 CC Rev A 020912 ii Procarta Immunossay User Manual For research use only Not for use in diagnostic procedures Trademarks a Affymetrix AW and Procarta are registered trademarks of Affymetrix Inc Luminex xMAP and xPonent are registered trademarks of the Luminex Corporation All other trademarks are the property of their respective owners Limited License Subject to the Affymetrix terms and conditions that govern your use of Affymetrix products Affymetrix grants you a non exclusive non transferable non sublicensable license to use this Affymetrix product only in accordance with the manual and written instructions provided by Affymetrix You understand and agree that except as expressly set forth in the Affymetrix terms and conditions no right or license to any patent or other intellectual property owned or licensable by Affymetrix is conveyed or implied by this Affymetrix product In particular no right or license is conveyed or implied to use this Affymetrix product in combination with a product not provided licensed or specifically recommended by Affymetrix for such use Citing Procarta Immunoassay in Publications When describing a procedure for publication using this product please refer to it as the Procarta Immunoassay from Affymetrix Disclaimer
9. Magnetic Plate Washer seal the 96 Well Plate with a new Plate Seal and shake at 500 rpm for 5 min at room temperature The assayed samples may take longer to read since there will be less beads in the wells Analyzing Results The concentration of the samples can be calculated by plotting the expected concentration of the standards against the MFI generated by each standard A 4PL or 5PL algorithm is recommended for the best curve fit Analyze the assayed samples according to the operation manual for the Luminex or Luminex based instrument 10 Procarta Immunoassay User Manual Troubleshooting Observation Probable Cause Recommend Solution Low Flow Rate Partial Blockage of the flow cell Remove the 96 well plate and perform a wash and rinse cycle Instrument needle is partially clogged Replace or clean needle according to the manufacturer s recommendations High CVs Samples and antigen standards not stored on ice Prepare the samples and standards on ice before setting up the assay Contamination from re using the Plate Seal Use a new Plate Seal for each incubation step Incomplete washing Blot the 96 Well Plate onto several layers of paper towels to remove any residual solution after each wash step Contamination from contents from adjacent wells Avoid splashing the Wash Buffer during wash steps into adjacent wells Poor pipetting techniques Use appropriate pip
10. Support For technical support contact the appropriate resource provided below based on your geographical location For an updated l st of FAQs and product support literature visit our website at www panomics com Location Contact Information North America 1 877 726 6642 option 1 then option 3 pqbhelp affymetrix com Europe 44 1628 552550 techsupport_europe affymetrix com Asia 81 3 6430 430 techsupport_asia affymetrix com About Procarta Immunoassay Kits Procarta Immunoassay Kits are available as Standard pre mixed panels a By Request user configured panels New custom assay development for analytes not listed on our website Procarta Immunoassay Kits contain all the reagents required to run the assays Please contact your local Affymetrix sales representative for new custom assay development for analytes not listed on our website www panomics com 2 Procarta Immunoassay User Manual How it Works Procarta Immunoassays use the xMAP technology multi analyte profiling beads to enable the detection and quantitation of multiple protein targets simultaneously in diverse matrices The xMAP system combines a flow cytometer fluorescent dyed microspheres beads dual laser design and digital signal processing to effectively allow multiplexing of up to 100 unique assays within a single sample The Procarta Immunoassay kits are compatible with all Luminex and Luminex based instruments currently avail
11. able Label with SAPE Detect captured protein Capture protein Read on Luminex Sample Antibody conjugated Antibody conjugated o beads beads e N Va 4 ao os s Antibody conjugated beads Biotinylated detection antibody Streptavidin SE SAPE 27 a A gt a ev A Incubate Incubate Incubate for 60 120 min for 30 min for 30 min Antibody conjugated beads Read signal using a Luminex instrument y L z E HU zn Dem ELI Procarta Immunoassay Kit Contents and Storage Conditions The Procarta Immunoassay Kit contains the following components listed below The kits are available in single 96 well plate or ten 96 well plate formats Refer to the Package Insert for quantities and details of components supplied The kits are shipped with blue ice Shelf life of the kit is 6 months from date of receipt when stored at 2 8 C The kits are also supplied with the following inserts m Packaging Insert Describes the products included in the kit Premixed Antigen Standard Insert Lists the lot number and reconstituted standard values Bead Analyte Association Insert Lists the bead region with the corresponding analyte Component Description Antigen Standards premixed lyophilized 2 Recombinant proteins in lyophilized powder Do not reuse discard vials each lot for a 1 plate kit after use Please note that more than 1 lot of vials may be shipped with ea
12. ch kit Review the Premixed Standard Insert prior to use Detection Antibody premixed Detection antibodies in aqueous buffered solution Antibody Magnetic Beads Capture antibodies conjugated to microspheres in aqueous buffered solution The Bead ID s are printed on the inside flap of the Kit Box Streptavidin PE SAPE Streptavidin conjugated R phycoerythrin in aqueous buffered solution 10X Wash Buffer Concentrated aqueous buffered solution Reading Buffer Aqueous buffered solution PCR 8 Tube Strip 0 2 mL polypropylene PCR 8 tube strip Procarta 96 well Flat Bottom Plate 96 well flat bottom microplate Plate Seals Adhesive backed foil plate sealer Contains sodium azide See WARNING below FI WARNING All chemicals should be considered potentially hazardous We recommend that this product and its components be handled by those trained in laboratory techniques and be used according to the principles of good laboratory practice E WARNING This kit contains small quantities of sodium azide Sodium azide is highly toxic and reactive in the pure form At this product s concentration though not classified as hazardous build up of sodium azide may react with lead and copper plumbing to form highly reactive explosive metal azide Dispose of the product in accordance with all State and local regulations 4 Procarta Immunoassay User Manual Required Equipment and Materials Not Supplied
13. ells Flat Bottom Plate B View the window with the bead regions and Doublet Discriminator DD gate The expected results are Signals for the expected beads should show up on the bead map Average bead count should be greater than 50 region The main single peak in the DD gate window should be within the set DD gates NOTE If you are running assays on your Luminex instrument that uses both Plates for Magnetic Beads and Filter Plates for Polystyrene Beads verify the probe height for each plate type before reading Example Plate Layout 13 Standards Samples 1 1 8 8 16 16 24 24 gt 32 3 2 9 9 17 17 25 95 35 33 3 3 10 10 18 18 26 26 34 34 4 4 11 11 19 19 27 27 35 35 5 5 12 12 20 20 28 28 36 36 6 6 13 13 21 21 29 29 37 37 7 7 14 14 22 22 30 30 38 38 Blank 15 15 23 23 31 31 39 39 Blank Plate Layout 1 2 10 11 12 A B C D E F G H 14 Procarta Immunoassay User Manual
14. etting techniques Use new pipette tips for each well during sample and standard addition Avoid touching pipette tips to sides of the wells when adding wash buffer 11 Observation Probable Cause Recommend Solution Low bead count Low signal or sensitivity Probe height is incorrect Refer to the Luminex manual for proper adjustment of the needle height Reading buffer volume added in the last step to resuspend the beads is too low Add 120 uL Reading Buffer into each well and shake at 500 rpm for 5 min at room temperature to resuspend the beads prior to reading on the Luminex instrument Make sure sample size is set at 100 uL in the acquisition protocol High bead aggregation Vortex the bead suspension well before using in the assay and ensure that the beads are properly mixed during the incubation steps Dyes contained in the beads are photo bleached from overexposure to light Store bead solution in the dark and protect the 96 Well Plate from light by wrapping the 96 Well Plate with aluminum foil Partial blockage of the flow cell Remove the 96 Well Plate and perform a wash and rinse to the instrument Instrument needle is partially clogged Replace or clean needle according to the manufacturer s recommendations Beads settle on the bottom of the well Confirm that the plate shaker is set to 500 rpm and shaking for at least 5 min before reading Did not use
15. fferent lot numbers at 2000 x g for 10 sec Add 250 uL of fresh cell culture media into one of the vials Incubate the vial on ice for 5 10 min Vortex gently for 30 sec Transfer the entire content into the second vial with a different lot number Incubate on ice for 5 10 min Vortex gently for 30 sec CON DU J W N s If more than 2 lots of antigen standards are in the kit repeat steps 5 7 until all the vials with different lot numbers are reconstituted NOTE For panels with liquid antigens follow the instructions on the Package Insert Step 2 Prepare 4 Fold Serial Dilution on IG mm m Prepare a 4 fold serial dilution of the reconstituted standard s using the PCR 8 tube strip provided Add 200 uL of the reconstituted antigen standards into the first tube of the strip tube and label as Standard 1 Std 1 Add 150 uL fresh cell culture media into Tubes 2 8 Using a P 200 pipette transfer 50 uL of the reconstituted antigen standards from Tube 1 into Tube 2 Mix by pipetting up and down for a total of 10 times After changing the pipette tip transfer 50 uL of the mixed standards from Tube 2 into Tube 3 Mix by pipette up and down 10 times Repeat Actions D to G for the rest of the tubes to prepare Std 4 8 Transfer 200 uL 50uL 50uL 50uL 50uL 50puL 50uL 50 uL Antigen Standard Vial Std1 Std2 Std3 Std4 Std5 Std6 Std7 Std 8 Performing the Assay Step Action Step 1 Prepare 1X
16. m temperature or overnight incubation at 4 C for assays that require higher sensitivities Step 7 Wash the 96 Well Plate A G Insert the 96 Well Plate into the Hand Held Magnetic Plate Washer and wait 2 min to allow the Antibody Magnetic Beads to accumulate on the bottom of each well Carefully remove the Plate Seal to avoid splashing the plate contents Remove the supernatant in the wells by quickly inverting the Hand Held Magnetic Plate Washer and 96 Well Plate assembly over a sink or waste container Add 150 uL of 1X Wash Buffer into each well Wait 30 sec to allow the Antibody Magnetic Beads to accumulate on the bottom of each well Remove the supernatant in the wells by quickly inverting the 96 Well Plate over a sink or waste container Repeat Actions D F 2 more times for a total of 3 washes After the last wash blot the assembly onto several layers of paper towels to remove any residual solution NOTE When washing the 96 Well Plate we recommend using a multi channel pipette or a multi channel automatic liquid dispenser Avoid touching the pipette tips to the sides of the wells when adding wash buffer using a multi channel pipette Step 8 Add Premixed A Add 25 uL of Detection Antibodies into each well Detection Antibodies B Seal the 96 Well Plate with a new Plate Seal C Remove the 96 Well Plate from the Hand Held Magnetic Plate Washer D Wrap the 96 Well Plate with aluminum foil and shake at 5
17. nstrument that uses both Plates for Magnetic Beads and Filter Plates for Polystyrene Beads verify the probe height for each plate type before reading Failure to adjust the probe height can cause damage to the instrument The Luminex system allows for calibration of Low and High RPI target values We recommend RP1 Low target value settings for Procarta Immunoassays Please refer to the label inside of the Kit Box or the Bead Analyte Association Insert for bead region analyte associations when entering the information into the Luminex acquisition software xPonent Bio Plex MasterPlex StarStation Please also refer to the Premixed Antigen Standard Insert when assigning the Standard 1 Std 1 concentration into the analysis software Each analyte may have a different Std 1 concentration For example if the starting concentration was 20 000 then a 4 fold dilution for Std 1 8 would be 20 000 5000 1250 312 78 19 5 4 8 and 1 2 pg ml NOTE If there is a malfunction of the Luminex instrument or software during the run the 96 Well Plate can be re read Remove the 96 Well Plate from the instrument insert the 96 Well Plate into the Hand Held Magnetic Plate Washer wait 2 min then remove the buffer in the wells by quickly inverting the 96 Well Plate over a sink or waste container Blot the assembly onto several layers of paper towels to remove any residual solution Resuspend the beads in 120 uL of Reading Buffer remove from the Hand Held
18. supplied 96 well microtiter plate Only use the Procarta 96 Well Flat Bottom Plate supplied with the kit Air bubble in the sample loop Standards not reconstituted and diluted correctly Refer to the Luminex manual for proper removal of the air bubble Prepare fresh antigen standards following the instructions in the Preparing Antigen Standards Section Expired reagents were used Reagents are good for 6 months from the date of receipt Do not use expired reagents Suboptimal assay conditions Follow the recommended incubation times and temperature Shake the 96 Well Plate during all incubations except during optional overnight incubation step Step 7D Poor accuracy Did not use the appropriate assay diluents Use the same sample type specific standard and assay buffers for standard and sample preparations Samples and antigen standards were not stored on ice Prepare and store the samples and standards on ice before setting up the assays 12 Procarta Immunoassay User Manual Validating the Hand Held Magnetic Plate Washer The Hand Held Magnetic Plate Washer is designed for use with the Procarta Immunoassay configured for the Procarta 96 Well Flat Bottom Plate This device uses magnetic separation to enable quick and easy processing of wash steps after each incubation This section describes how to validate the hand held washer prior to running an experiment and how to operate the device
19. to remove any residual solution AT position Securing tabs Ld a Pa Step 4 Wash Antibody A Add 150 uL of 1X Wash Buffer into each well Magnetic Beads B Wait 30 sec to allow the Antibody Magnetic Beads to accumulate on the bottom of each well C Remove the wash buffer in the wells by quickly inverting the Hand Held Magnetic Washer and 96 Well Plate assembly over a sink or waste container D Blot the inverted assembly onto several layers of paper towels to remove any residual solution Step 5 Add Antigen Add 50 uL of standards or 50 uL of samples as marked on the plate layout sheet into each well For Standards and Samples blanks add 50 uL of cell culture media 8 Procarta Immunoassay User Manual Step Action Step 6 Incubate the 96 Well Plate Seal the 96 Well Plate using a Plate Seal provided Remove the 96 Well Plate from the Hand Held Magnetic Plate Washer and completely wrap the 96 Well Plate with aluminum foil Room temperature incubation 1 Shake the 96 Well Plate at 500 rom for 60 to 120 min at room temperature 2 Proceed to step 7 Alternatively the 96 Well Plate can be incubated overnight 1 Shake the 96 Well Plate at room temperature for 30 min 2 Transfer the plate to 4 C and store on a level surface 3 After incubation remove the 96 Well Plate from 4 C and shake for 30 min at room temperature 4 Proceed to step 7 NOTE We recommend 120min at roo
20. y detect the active form of TGF B The samples must be acid treated and then neutralized to convert the complexed form of TGF to its active form The assay should be processed as a single plex assay since the sample must be acid treated The TGF P sample preparation protocol can be found on our website www panomics com 6 Procarta Immunoassay User Manual Preparing Antigen Standards This section provides instructions on how to make a 4 fold 8 point standard curve for the assay panel The antigen standards should be prepared after sample preparation is completed The serially diluted antigen standards should be added to the assay plate at the same time the samples are added Eachl plate kit is shipped with two vials of identical antigen standards from the same lot In some cases an additional set s of standards from a different lot may be included in the kit Please refer to the Premixed Antigen Standard Insert when assigning the Standard 1 Standard 8 antigen values for each analyte Step Action Step 1 Reconstitute Lyophilized Antigen Standards A Instructions for assay panels with only 1 standard lot in the kit 1 Centrifuge the antigen standard vial at 2000 x g for 10 sec 2 Add 250 uL of fresh cell culture media into the vial 3 Vortex gently for 30 sec 4 Incubate on ice for 5 10 min Instructions for assay panels with more than one standard lots in the kit Centrifuge all the antigen standard vials with di

ProCartaMagnetic-Cell Culture.book

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