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1. After the instrument is dry wait until it returns room temperature and then fasten the nozzle cylinder and reassemble the ejector pipe into the body Note When reassembling be careful not to put the seal ring and O ring in the wrong order If they are put together in the wrong order it not only affects airtightness but causes liquid to leak inaccuracy failure in extracting liquid etc Note If the instrument is reassembled when it is still warm it may cause breakdown or deterioration of the instrument such as breakage of the screw threads etc Be sure to reassemble the instrument after it has completely cooled down If the instrument is used when it is warm accurate liquid handling can not be carried out Fit the ejector pipe to the body e 2ul 1000 ul Fig F While pressing down the ejector button with your finger so that the metal stay sticks out from the center of the body insert the ejector pipe into the body and fasten it with the setscrew e 5000 ul 10 ml Fig H Fig While pressing down the ejector button with your finger insert the ejector pipe Note Don t touch the instrument directly with your hands just after it into the body so that its hole meets the tapped hole on the metal stay and is dry because it will have gotten very hot during drying Directly fasten the ejecto2. of wide sampling of 8 kinds of liquid from 0 1 ul to 10 000 ul each The instrument has a special structure so that its precision is hardly affected by the temperature of your hands if the instrument is used for long hours Since PTFE fluoroplastic is used in the airtight chamber of the instrument the instrument keeps airtightness and precise reproducibility for long hours The tip of the ejector pipe reaches the bottom of a general test tube 110 mm deep due to its thinness Organic solvents can be pipetted by this instrument if the O ring for organic solvent supplied as an option is attached to it The tip can be removed without touching by hand thanks to the tip ejection mechanism Moreover there is no fear of breakage of test tubes etc caused by tip ejection because the tip ejection part is made of plastic Standard accessories Three tips one tip for NPL 5000 10ML Three filters for NPL 1000 5000 10mL One filter replacing tool for NPL 1000 5000 10mL When unpacking the package check to make sure that the above mentioned items are included Precautions on safety For using your Nichipet EX Plus properly and safely carefully read Precautions on safety in this paragraph and CAUTIONS on the next page before starting work with it The contents of CAUTIONS are matters that require the user s attention not only for using the Nichipet EX Plus properly but for preventing the user from accidents an
3. 00 ul or more gt iy to screw the nozzle cylinder firmly into the body again ey Replace the filter with a new on supplied as a standard accessory or purchase an optional filter set for replacement ND The O ring and seal ring are assembled in the wrong order W Reset the O ring and seal ring set according to the instructions in Disassembling reassembling wary a gt a Ne J N Ke The O ring or and seal ring gt Purchase an optional O ring and aN set are worn seal ring set for replacement XX JO NS The nozzle cylinder has Screw the nozzle cylinder firmly come loose J ue the body again the nozzle cylinder is worn A j r Purchase an optional nozzle cylin stepped wear can be hecked bi der for replacement Extracted liquid leaks ee REN NE E Ne from the tip he O ring and seal ring set sides an optional O ring and is worn because the plung A i g er is damaged or rusty seal ring set for replacement JON e tip is loosely sacs gt ase ane the tip to the S J oht again E N a i the ush button does not work pl well just after extracting liquid or it is just sticking to the body disas semble the instrument and wash The push button Liquid has been sucked clean ie part oe down ev operates poorly into the main body ery part with a soft cloth If there are some parts getting rus ty or co
4. For repair service or information you may contact your local distributor MANUFACTURER NICHIRYO 4 10 lwamoto cho 2 chome Chiyoda ku Tokyo 101 0032 Japan TEL 81 3 5820 2664 FAX 81 3 5280 2410 Homepage URL _http www nichiryo co jp E mail i sales nichiryo co jp 00 2004 3 2000 Autoclavable Nichipet EX Plus Digital micro pipette for liquid handling User s Manual CERTIFIED ISO9001 e Thank you for your purchase of our Nichipet EX Plus e Before proceeding to work with your Nichipet EX Plus carefully read this manual for proper use of it Always Pursuing Originality Since 1944 NICHIRYO Autoclavable Nichipet EX Plus Digital micro pipette for liquid handling Features The main body of the Nichipet EX Plus is autoclavable in whole at 121 C for 20 minutes The Nichipet EX Plus is suitable for liquid handling on a clean bench because it is designed to be usable in irradiation of ultraviolet rays If ultraviolet rays are applied to the Nichipet EX Plus for a considerably long time it may become discolored but nothing affects its performance Since the grip structure is easy to grasp the user hardly gets tired when handling liquid for many hours The sample volume can easily be set by simply turning the push button while looking at the digital indicator Setting of the sample volume can easily be locked with one touch one touch lock mechanism The instrument is capable
5. arts one after another e 2ul 1000 ul Fig F Remove the plunger single spring O ring retainer O ring and seal ring from the nozzle cylinder 3 The shape of the O ring retainer differs depending on the capacity of the instrument Fig G e For the 20 ul type remove the fluoroplastic PTFE spacer that is inside e 5000 ul 10 ml Figure H Fig Remove the O ring retainer and seal ring from the nozzle cylinder Note Take care not to lose small parts during disassembling 2 Reassembling Reassemble the nozzle cylinder e 2 ul 1000 ul Fig F First set the single spring on the plunger next set the O ring retainer seal ring and O ring in this order Then insert the assembled plunger into the nozzle cylinder and screw it into the body e 5000 ul 10 mi Fig H Fig me Drying the instrument Set the seal ring O ring on the plunger in this order and then insert the as Dry the instrument immediately after autoclaving is complete It is necessary to dry sembled plunger into the nozzle cylinder while taking care that the O ring the instrument with a constant temperature air drier at 60 C for 60 minutes or longer does not come off the center After insertion screw the nozzle cylinder into the body Pull out the ejector pipe referring to Disassembling on page 6 Turn the nozzle cylinder counterclockwise by two and a half turns to loosen it Put the instrument in a constant temperature air drier for drying
6. bration certificate Because temperature and humidity conditions affect the calibration results of liquid measurement devices your pipette should be calibrated under conditions of use The calibration results obtained in your laboratory may vary from our results due to differences in environmental testing conditions 16 Tips autoclavable Code eet Color Applicable model Tip length aes a BMT UT 0 1 10 Clear NPL 2 31 0 mm 1000 BMT SS 0 5 10 Clear NPL 10 45 5 mm 1000 BMT SE 2 200 Clear NPL 20 100 200 51 1 mm 1000 BMT SG 2 200 Clear NPL 20 100 200 53 0 mm 1000 BMT L 100 1000 Blue NPL 1000 77 0 mm 1000 BMT X 1000 5000 Green NPL 5000 132 0 mm 200 BMT Z 1000 10000 Clear NPL 10ML 158 0 mm 200 Rack tips autoclavable Code Variable Color Applicable model Number of pieces a lot capacity ul BMT UTR 0 1 10 Clear NPL 2 960 96pcs X 10cases BMT SSR 0 5 10 Clear NPL 10 960 96pcs X 10cases 96pcs X 10cases BMT SER 2 200 Clear NPL 20 100 200 960 BMT SGR 2 200 Clear NPL 20 100 200 960 96pcs X 10cases BMT LR 100 1000 Blue NPL 1000 1000 100pcs X 10cases BMT XR 1000 5000 Green NPL 5000 100 50pcs X 2cases 15 Operating procedure 1 Setting the liquid volume 1 Turn the lock handle in the unlocking direction to loosen it Fig A 2 Turn the push button to set the digital counter to a des
7. care not to touch the tip Note Don t extract liquid with the push button depressed at point c shown in the Fig C Note Be careful to operate the push button very gently If it is rapidly released the liquid may possibly be sucked into the main body and pipetting may end with an inaccurate result 3 Discharging the liquid 1 Gently place the tip on the inner wall of a proper vessel Fig D 2 Gently press the push button down from point a to point b Five seconds later press down the push button again from point b to point c The liquid is dis charged from the tip Fig D 3 Press the ejector button to remove the used tip from the instrument F ig D When handling liquid harmful to humans be careful not to touch A the tip during and after operation Push button To lock To unlock gt Lock handle Ejector button Fig A Digital counter 20 200 ul 0 2 2 0 Q Point mark 9 Fig B en Lear a b g ES S E gt Fig C Bn O Fig D Replacement parts list When placing an order make sure to specify the capacity of each item Name of replacement part Contents of set Capacity Capacity ul Plunger set Plunger head Plunger 2 10 20 100 200 1000ul N Plunger assembly Plunge
8. d physical damage After reading this manual please keep it in a convenient place for refering to at any time ate Memo 18 Memo Please read the following prior to use for your safety and correct usage Z CAUTIONS Be sure to observe the following instructions for using your Nichipet EX Plus properly and safely If the user uses the Nichipet EX Plus in the wrong way disregarding the following instructions it may result in injury to the user or and other persons or physical damage to this instrument or and other equipment 1 Don t use this instrument for any purpose except pipetting fractionizing liquid 2 Don t modify this instrument because modification may cause an accident 3 Carefully handle the filter replacing tool with particular attention to its sharpened tip 4 Don t use this instrument for pipetting any liquid to be injected into the human body 5 Don t discharge any sample liquid while pointing the instrument at anybody because some kinds of liquid are harmful to humans 6 Don t eject the tip towards anybody 7 Don t eject the tip with liquid inside 8 Carefully handle the instrument and tip because the tip is sharply pointed 9 Firmly fit the tip to the nozzle otherwise the tip may fall off and the inside sample liquid may scatter 10 If the instrument is soiled with liquid harmful to the human body immediately take proper measures to clean it safely before proceeding with work 11 When handling liqu
9. id harmful to the human body be careful not to directly touch the tip during or after use 12 Don t use this instrument for stirring liquid and so on otherwise not only the tip may be loose and fall off but the instrument may be soiled with the scattered liquid 13 Regarding the model NPL 10 ul the plunger sticks out of the tip of the nozzle cylinder when the push button is completely depressed Therefore don t press the push button with your finger or anything else on the tip of the nozzle cylinder 14 Since the instrument is extremely hot when it is autoclaved or dried be careful not to touch it directly with your hand just after such treatment otherwise it may cause a burn on the hand or another accident 15 When pipetting an organic solvent with this instrument use the O ring for organic solvent optional 16 Although this instrument has good chemical resistance in general it may be damaged by some kinds of chemicals such as N methyl pyrrolidinone etc When using a special chemical please make enquiries to our company 247 2 10 11 Matters that require strict observance Users are required to strictly observe the following points in order for the instrument to keep its excellent precision reproducibility and original performance for a long time Don t expose this instrument directly to the sun when working with it or for 2 hours before starting work otherwise the instrument may fail in precise pi
10. ired liquid volume To increase the setting volume turn the push button to pass the last set division on the scale by half a turn and then set at the higher graduation as desired To decrease the setting volume reset the digital counter directly at the lower gradu ation as desired When setting the liquid volume set the counter s graduation at the point mark red appearing in the lower part of the counter window Fig B 3 After setting the liquid volume turn the lock handle in the locking direction to lock it Fig A Note Don t exceed the specified liquid volume limit otherwise the instrument may be damaged or deteriorate in the quality 2 Extracting liquid 1 Attach a disposable tip to the nozzle We recommend that you attach it from the rack tip 2 Press the push button down from point a to the point b Fig C 3 While depressing the push button immerse the tip into the liquid to the extract ing volume approximately 3 mm deep Fig D 4 Pull up the push button to point a to suck the liquid into the tip With the tip still immersed in the liquid keep the instrument stationary for about 1 second to wait untill the liquid is completely sucked into the tip Fig D 5 Gently extract the instrument to separate the tip from the liquid so that there are no drops of liquid left on the outside of the tip If there are some drops left on the outside of the tip wipe them off with a tissue or the like taking
11. merical values volume are meant as a guide only When checking volume be sure to confirm using a calibrated balance 10
12. nd the instrument may deteriorate When autoclaving do not place pipettes one on top of the other in the autoclave or stand them at angles with the nozzles facing down so that a load is applied on the nozzles This instrument is made of an autoclave compatible material but because the temperature inside sterilizers is high there is a risk that parts subject to loads will be deformed After autoclaving and drying the instrument leave it untill it gets completely cool before using again If the instrument is used when warm the accuracy may not come up to the standard level After autoclaving and drying the instrument assemble the instrument after it is completely cooled If it is assembled when it is still hot it may cause deterioration in the instrument such as breakage of the screw threads When turning the push button don t exceed the specified sample volume limit otherwise the instrument may be damaged or deteriorate Don t perform pipetting with less liquid than the set volume If the quantity of liquid is less than the set volume it may cause the liquid to scatter into the main body and the instrument may deteriorate in quality ee Inspection and Calibration Statement The enclosed pipette was tested and calibrated under closely controlled environmental conditions to ensure that it meets published calibration specifications The precision and accuracy results obtained for this pipette are provided on the enclosed cali
13. ndle to the LOCK side and lock securely so that the counter does not move 2 Hold the unit in one hand and hold the push button with your fingers so that it does not turn Fig J 3 Insert the adjustment tool s tabs into the two grooves on the adjustment head Fig K 4 Carry out fine adjustment of volume by turning the adjustment tool to the Fig J left and right Using the adjustment tool s point mark and the push button s graduation marks as a guide will allow easy adjustment of the necessary volume 5 Please refer to the Volume guide chart below for the necessary fine adjustment volume Note Be sure to lock the counter securely Note Volume adjustment A Turning clockwise decreases volume B Increase Graduation mark A Decrease Adjustment tool Point mark Adjustment head Fig K B Turning counter clockwise increases volume Volume guide for turning moving with the NPL Nichipet EX Plus volume variable tool Unit ul Code 1 2 graduation 1 graduation 5 graduation 10 graduation Model No mark mark marks marks NPL 2 0 0013 0 0026 0 013 0 026 NPL 10 0 0065 0 013 0 065 0 132 NPL 20 0 013 0 026 0 131 0 263 NPL 100 0 067 0 133 0 665 1 330 NPL 200 0 132 0 264 1 322 2 644 NPL 1000 0 659 1 319 6 597 13 194 NPL 5000 3 332 6 663 33 316 66 633 NPL 10ML 6 696 13 393 66 966 133 932 The above nu
14. nto the nozzle A Don ttouch a filter that is polluted with liquid harmful to humans The filter replacing tool for the filters for the 5000 ul and 10 ml types is dangerous because of its sharpened tip 1000 yl Filter replacing pincers Filter 5000 ul 10 ml Filter replacing tool Filter Fig L Autoclaving instrument This instrument is autoclavable When autoclaving carry it out at 121 C for 20 min utes following the procedure mentioned below For the 1000 ul 5000 ul and 10 ml types remove the filter referring to the Filter replacement procedure above Release the lock handle from the locked position and set the counter graduation to the allowable limit of the liquid volume After autoclaving is complete dry the instrument completely 11 Nozzle cylinder Nozzle Plunger head Single spring Ejector setscrew O ring retainer Ejector pipe Plunger Seal ring O ring i Fig F Orientation of seal ring p p C 10 ul 20 ul q q 0 100 yl 200 wl 1000 pl Fig G Body Plunger head Plunger assembly Plunger Seal ring O ring Setscrew Nozzle cylinder Ejector pipe Fig H Plunger Set screw head Plunger assembly Plunger Seal ring O ring Set screw Nozzle cylinder Ejector pipe Fig Ge Calibration Carry out calibration as follows 1 Turn the lock ha
15. petting Avoid working with this instrument in a humid and hot place Just before starting work with this instrument avoid touching the tip and nozzle cylinder as far as circumstances permit If the nozzle cylinder is warmed by your hand it is hard to maintain accuracy For fractional pipetting follow the forward method the way explained in this manual If it is performed in a different way it may result in inaccurate pipetting Operate the push button very gently If it is quickly released it may result in not only inaccurate pipetting but also deterioration in the instrument because the sample liquid is sucked into the main body To prevent the instrument from malfunction inaccuracy and contamination a filter is attached to models NPL 1000 5000 and 10ML A filter is supplied at time of purchase Don t reuse a tip that has been used once and carefully dispose of the used tip If a tip is repeatedly used it may cause inaccurate and impure pipetting and cross contamination among samples For example if the previous sample liquid remains inside the tip it is mixed in with new sample liquid and the new sample is contaminated by the previous one Therefore pipetting of the next sample ends with a wrong result This phenomenon is called mutual contamination of samples Don t hold the instrument horizontally or upside down when there is liquid inside the tip otherwise the liquid gets into the main body a
16. r Joint block Joint plate Joint shaft 5000ul 10ml Single spring Common to all capacities Nozzle cylinder Common to all capacities O ring seal ring set O ring seal ring 2 10 20 100 200 1000ul O ring seal ring set O ring seal ring 5000ul 10ml N o ajA w O ring seal ring set for organic solvent O ring for organic solvent Seal ring O ring retainer 2 10 20 100 200 1000ul O ring seal ring set for organic solvent O ring for organic solvent Seal ring 5000ul 10ml O Ejector pipe Common to all capacities Spring Washer 10 Ejector setscrew set 2 10 20 100 200 1000ul 11 Ejector setscrew 3 pcs 5000u 12 Ejector setscrew 4 pcs 10ml 13 Filter 10 pcs 1000u 14 Filter 100 pcs 5000ul 10ml 15 Filter replacing tool 1000 5000ul 10ml Specifications of the instruments and optional accessories as well as contents of ac cessory sets are subject to change without notice No part of this manual may be reproduced reprinted in any form without permission of the copyright holder This is strictly prohibited by the copyright law 14 Symptom Troubleshooting Possible cause Remedy The tip cannot be N ejected o o A The instrument fails to extract liquid YNZ yJ 7J va The nozzle cylinder has come loose The filter has soaked up lauid 10
17. r pipe with the setscrew s touching the hot instrument may cause injury Note After reassembling repeat trial operation several times and check to see that there is nothing wrong with the instrument Code Variable capacity Liquid volume Accuracy Reproducibility Model No ul ul 04 NPL 2 0 1 2 1 45 0 lt 2 5 2 43 0 lt 1 0 05 15 0 lt 3 0 NPL 10 0 5 10 5 1 0 lt 1 0 10 1 0 lt 0 5 2 15 0 lt 3 0 NPL 20 2 20 10 71 0 lt 1 0 20 1 0 lt 0 4 10 42 0 lt 1 0 NPL 100 10 100 50 1 0 lt 0 3 100 10 8 lt 0 3 20 1 0 lt 0 5 NPL 200 20 200 100 40 8 lt 0 3 200 40 8 lt 0 2 100 1 0 lt 0 5 NPL 1000 100 1000 500 40 8 lt 0 3 1000 40 7 lt 0 2 1000 1 0 lt 0 3 NPL 5000 1000 5000 2500 40 8 lt 0 3 5000 10 6 lt 0 2 1000 42 0 lt 0 4 NPL 10ML 1000 10000 5000 40 8 lt 0 3 10000 40 4 lt 0 2 Fig E When the liquid volume in the NPL 2 is 0 2 ul or less its accuracy and reproduc ibility are greatly affected by the operator s sampling skill se 12 Filter replacement procedure 1000 ul Fig L O Insert the tips of the filter replacing pincers into the two notches on the sides of the filter and pull the filter in the direction of the arrow Set the projection of a new filter in the internal groove of the nozzle and then press the filter into the nozzle 5000 ul 10 ml Fig L Insert the filter replacing tool into the filter and pull in the direction of the arrow Insert a new filter i
18. rroded inside the body re place the parts with new ones by purchasing optional replacement parts f there is still something wrong with the instrument after checking the above men tioned immediately stop using the instrument and ask us or our agent to repair it Before bringing the instrument for repair be sure to check whether it has been polluted with microbes or matter harmful to humans DR Disassembling Reassembling the airtight chamber If such symptoms as mentioned in Troubleshooting page 13 is occur disas semble and inspect the instrument according to the following procedures 1 Disassembling Remove the ejector pipe setscrew e 2ul 200 ul Figure E Turn the ejector pipe setscrew in the direction of the arrow to remove it and then pull out the ejector pipe in the direction of the arrow e 5000 ul Figure E Remove the three ejector pipe setscrews with a Phillips head screwdriver and then pull out the ejector pipe in the direction of the arrow 10 ml Figure E Remove the ejector pipe setscrew with a Phillips head screwdriver and then pull out the ejector pipe in the direction of the arrow Turn the nozzle cylinder counterclockwise to remove it as it is screwed into the main body Note When removing the nozzle cylinder take care with the internal parts as some of them occasionally spring out of the body for types of 2 ul to 1000 ul capacity Remove internal p
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