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Neurophore BH-2 Mico-Iontophoresis System User`s Manual

1. 12 PPM 2 Gas Input Connections Advantages of Micro Pressure Ejection 13 Pressure Ejection Into Saline 13 14 Seven Barrel Micropipette ccccccsscssssssseees 15 Alignment Procedure BH 2 Balance Module cccccceeseesseeeeeees 16 IP 2 Module Alignment and Control Settings 17 Bal Module Alignment and Control Settings 17 Final Adjustment and Control Settings 18 Addendum to Neurophore BH 2 Manual 19 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Warranty and Repair Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above li
2. 2 is comprised of a precise pressure regulator digital display transducer and a timing mode switch It connects to an external pressure source such as a com pressed bottle of Nz can be set to provide continuous or periodic pressure pulses ranging 0 to 30 psi with 0 to 0 5V proportional to the output pressure as well as a sync pulse coincident with the pressure start cycle Ejection Timing and Mode Switch The mode switch provides five push button controls which include operations such as Cycle Trigger Gate continuous and termination Cycle Mode In the cycle mode after selecting single or recycle operation the start push button will initiate the ejection pumping action In this mode each succeeding pump module is automatically triggered after the pause time of the preceding event has been complet ed Both eject and pulse times can be present to cover a range from 1 to 99 seconds with a 1 second resolution Trigger Mode When the Trig switch is energized the eject time interval will be started by virtue of an externally applied trigger pulse fed to the respective inputs on the control panel Eject timing interval can be preset covering a range of 1 to 99 seconds with a 1 sec ond resolution Gate Mode When the Gate switch is energized pumping action will be started by virtue of a gate input signal applied to the respective trigger gate terminals on the control panel The eject and pause time settings are not operative in this mode
3. 4a Retention Ejection Current Polarity Switch 7a Eject and Pause Time Indicator 6a Ejection Current Control 10 turn Pot 3a Retention Control 0 50 nA RETENTION EJECTION CYCLE TRIGG GATE CONT TERM 100Mc 9a Eject Mode Switch Cycle Trigger Gate Continuous Termination 8a Eject amp Pause time digital preset switch Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual ANALOG push V SYNC mV P 10a Sync Output Incident with Eject Time Interval 11a Analog Input 5 mV nA Ext Signal Drive Input 13a Pipette Voltage 12a Analog Output 5 mV nA Display Switch IP 2 Pump Module Controls Publication 5403 003 REV B S Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual PPM 2 Pneumatic Pump Module The pneumatic pump module has been designed specifically for pressure injection of drugs in pharmacological studies of drug evoked synaptic discharges Emphasis has been given in the design as to pressure control and regulation 0 to 30 psi or 0 to 2 2 kg cm2 as well as precise timing capability The Pneumatic Pressure Module PPM 2 is compatible with the BH 2 NeuroPhore microiontophoresis main frame By virtue of interchanging the iontophoresis pump module P 2 with a pneumatic pressure pump PPM 2 the overall system capability can be expanded for simultaneous pressure and iontophoretic injection of drugs from a multibarrel pipette The PPM
4. each module be used for marking information such as type of drug barrel resistance retention and ejection values etc since such information can be very useful during control of the experiment After the resistance value of each pipette has been determined it is recommended that the resistance toggle switch be returned to center or inactive position The above pro cedure should be repeated for each active barrel Publication 5403 003 REV B 5 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Recommended Setup Procedure Setting of Retention Current a Press either Cycle Trigger of Gate button on the Eject Mode switch 9A p 8 b Set the retention polarity switch 4A p 8 to coincide with the retention required of each drug and set the retention current control GA p 8 for the desired current magnitude The retention current and polarity can be read on the digital display Setting of Ejection Current a Set the EJECT Mode switch 9A p 8 to CONT mode and adjust the control 6A p 8 to the desired current magnitude b Set the EJECT Mode switch QA p 8 to CYCLE or any other mode if external timing is required c Select the appropriate Eject and Pause time intervals on each of the thumb wheel switches 8A p 8 d Select the appropriate Time Unit by setting switch on the BM 2 Balance Module 9B p 8 C1 sec or 10 ms e Select Single or Recycle Mode Switch 8B p 6 for desir
5. ejection control to zero CCW Repeat step e for all IP 2 modules Press Gate button on all IP 2 s Turn ejection controls fully clockwise on all IP 2 s Set range switches to 500 nA on all IP 2 s Depress CONT button on one IP 2 and adjust VR102 to obtain 001 reading on balance display Depress GATE button Repeat step f on all IP 2 s Publication 5403 003 REV B 3 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Addendum to Neurophore BH 2 Manual Ref Pipette Voltage Display All IP 2 Modules manufactured after December 1981 are fitted with an additional push button switch This switch is marked Push V and it is mounted above the module han dles between the ANALOG and the SYNC OUT connectors Depressing this switch will cause the digital display to read the voltage across the pipette equal to the current flow through the pipette times the pipette impedance G e by Ohms Law V R D The voltage readout will function whenever the Push V button is depressed regard less of the position of other controls Therefore the pipette voltage can be measured during the drug ejection retention or impedance checks Specifications Resolution 1 10 Volt Maximum Range 99 9 Volt
6. 2 Advantages of Micro Pressure Ejection a Pressure ejection can be used to administer drugs which are difficult to deliv er with conventional microiontophoretic techniques b Does not share the leakage and warm up problems associated with microion tophoretic techniques c Micropressure ejection may be more amiable to dose dependent relation ships d Offer linear and reproducible delivery characteristics See illustrations Pressure Ejection into Saline Figure 1 The effect of increasing ejection time on the release of 3H sucrose 3H Sucrose was pressure ejected from a multibarreled pipette at 5 open circles 10 closed squares 20 open triangles or 30 closed circles psi of nitrogen The ordi nate represents total counts per minute tritium collected minus the blank 10 CPM BK Pressure Ejection into Saline e 20 PSI 10 PSI 5 PSI a 10 20 30 TIME SEC 3 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual PPM 2 Gas Input Connections Figure 2 The effect of increasing ejection pressure on the release of 3H sucrose 3H sucrose was pressure ejected from a _ multibarreled pipette in 1 open circles 2 closed squares 10 open trian gles or 30 closed circles second pulses The ordinate represents total counts per minute tritium col lected minus the blank 10 CPM BK Figure 3 Dose dependent inhibition of corti cal neuronal
7. Neurophore BH 2 Micro lontophoresis System User s Manual BH 2 Mainframe Chassis MS 2B Power Supply BM 2 Balance Module IP 2 lontophoresis Pump Module PPM 2 Pneumatic Pump Module MAI 65 0200 MA1 65 0600 MAI 65 0201 MA1 65 0601 MAI 65 0202 MA1 65 0602 MAI 65 0203 MA1 65 0603 MAI 65 0204 MA1 65 0604 HARVARD APPARATUS Publication 5403 003 REV B WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monit
8. V full scale in kg cm setting Lemo miniature connector TTL pulse time incident with output pressure pulse Red LED Green LED 47 x 21 x 35 cm 19 x 8 75 x 14 in Wx Hx D System 8 2 kg 18 lbs Power Supply 11 kg 24 Ibs E Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual System Description BH 2 Mainframe and Balance Module The BH 2 mainframe is designed to be 19 inch rack mounted and is prewired to accept one balance and a combination of five IP 2 pump modules and or pneumatic pressure modules any combination of five plus balance module The balance module in addi tion to providing current neutralization automatic feedback and control of inverse sum of all pump currents has independent capability of current pump settings with a working range of 0 500 nanoamperes The module includes a digital display time clock provisions for electrical and manual Cycle Start Stop and Single Cycle Recycle switch Trigger and Gate input terminals to initiate externally controlled Eject pump ing action of respective modules Analog input for balance or drive with override capability Analog output for monitoring of unbalanced currents MS 2 Power Supply The AC power supply is self contained in a rack mount cabinet and provides all volt ages required to operate the NeuroPhore System The power supply interconnects to the mainframe via flexible cable The supply works off either 115 or 220 VAC 50 or 60 Hz s
9. a b d e 1 2 3 4 5 pin 6 U102 by adjusting VR103 IP 2 Module Ground point B R172 left pin set point A R165 right pin to OV zero voltage by adjusting VR106 Ground point A R165 right pin set point B R172 left pin to OV zero voltage by adjusting VR105 Repeat b and c two or three times for better adjustment Adjust VR104 to obtain 000 reading on digital display Balance Module Alignment amp Control Setting Test Conditions Balance module alone No IP 2 s Output connector terminated by 10 Mohms resistor Unbal current current pump switch in unbal position Polarity switch in center OFF posi tion Time Unit switch in 10 ms posi tion Adjusting Procedure a b c d e fy D h D Set R104 dower pin for OV zero voltage by adjusting VR 103 Ground point B R109 left pin set TO OUTPUT CONNECTOR PIN NOgi 7x50M A Ry TO OSCILLOSCOP External Load for Testing and Adjustment point A R105 right pin to OV zero voltage by adjusting VR 101 Ground point A R105 right pin set point B R109 left pin to OV zero voltage by adjusting VR 102 Repeat b and c two or three times for better adjustment Set OV on display by turning VR 104 Obtain 000 reading by adjusting VR 105 Set VR 107 to obtain 2 000V Imv at pin 2 U 27 Using voltmeter set termina
10. atients Publication 5403 003 REV B s Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Introduction Micro Iontophoresis of ionized drugs from multi barreled electrodes has evolved into a practical method of testing electrophysiological action of putative neurohormones The NeuroPhore BH 2 System has been designed to facilitate the most stringent requirements and provides precise stimulation and quantitative control for ejection of drugs in pharmaceutical studies of drug evoked neurosynaptic discharges The BH 2 System has been developed with the guidance of active researchers with extensive experience in Ionto Phoresis techniques Emphasis has been given in the design of this system to reliability performance compliance to accommodate high impedance multi barreled micro pipettes and to simplicity of operation The NeuroPhore BH 2 is modular and the system is comprised of the following assemblies BH 2 Mainframe amp BM 2 Balance PPM 2 Pneumatic Pump Module Module MS 2 Power Supply IP 2 Pump Module MS 7PB MS 7MT Seven Barrel Pipett Features noe Modular Design pause timing Will accommodate 7 barrel Injection and retention currents and pipette electrode resistance readout with All Solid State digital display Unbalance and out of compliance 105V compliance no batteries auto indicators Automatic Current Neutralization External analog input control over Digitally control
11. de start switch or external trigger initiates each cycle in recycle mode once start switch or external trigger is actuated repetitive cycles commence automatically Two basic time units selectable 10 msec or 1 sec in 10 msec position eject and pause time switches of IP 2 modules can be set to cover time range from 0 to 990 msec with 10 msec resolu tion in sec position time scale expanded from 0 to 99 sec with one sec resolution Cycle start stop trigger gate 1 through 5 banana jacks termi nals floating input optically coupled input voltage 5 to 15 V TTL compatible Lemo miniature receptacle ground referenced 5mV nA input impedance 100 Q Lemo miniature receptacle 5mV nA ground referenced Lemo miniature receptacle TTL pulse Seven pin miniature connector mates with ultra flexible cable leading to microelectrode holder Source Gas Air or nitrogen recommended no explosive or combustible gases Max Input Pressure 125 psig 7 8 kg cm Input Filter 5 micron element Output Pressure Output Pressure Display Min Pressure Pulse Width Max Pressure Pulse Width Gas Input and Output Couplings Analog Output Sync Output Eject Time Indic Pause Time Indic Dimensions Weight 0 to 99 9 psig 0 to 7 kg cm Three decimal digits 30 msec 99 sec 990 sec optional Quick connect type Lemo miniature connector voltage proportional to output pressure 0 to 999 mV full scale in psig setting 0 to 700 m
12. discharge induced by micropressure ejection of 2dA in the absence of regularly repeated pulses of drug A B and C Ratemeter records from a single cerebral cortical neuron showing the magnitude of inhibitions elicit ed by applying various pressures to the 2dA filled barrel Duration of PRESSURE PSI ejection is shown by the underly ing bar Percent inhibition is indicated above each 2dA response D Dose depend ent inhibitions produced in one cell The 2dA is applied with large variations in intere jection time over a 60 minute period 5 10 20 30 Figure 4 Ratemeter records from two neurons demonstrating typical controls for the physio logical effects of micropressure ejected drugs 2dA caused marked inhibitions of Purkinje cell firing rate when applied at 20 psi for 2 seconds Figure 5 Reproducibility of the micropressure ejection technique A An average of 1057 24 counts per minute of tritium was collected from five separate pressure ejections of 3H sucrose from one pipette each applied at 20 psi for five seconds Vertical line rep resents one standard error of the mean B and C Ratemeter records from two cere bellar Purkinje cells which illustrate the reproducible physiological responses to repeated micropressure ejections of drugs at two second pulses of 1 psi underlying bars NE B or GABA C when repeatedly applied produced uniform inhibitions of neuronal activity which did not vary for periods of time of u
13. ed timing f Press Cycle Stop switch 5B p 6 to release latch and then depress Cycle Start Switch to initiate cycle run Setup of Automatic Balance Set Automatic Balance and Current Pump selector switch 3B p 6 to the unbalance current position The 3 digit display will indicate a reading close to zero 0 001 or 2 nanoamperes For fine control adjustment it is recommended that the pump current and polarity selector to be switched to either positive or negative position opposite to the polarity indicated on the digital display Adjust the pump current control 2B p 6 to as close to zero as possible NOTE The slight variations may be noted such as a jump of one digit This variation is attributed to the inherent noise of A D converter 3 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Recommended Setup Procedure Setup of Pneumatic Pressure Module 1 2 Connect the gas input pressure as shown below Push the TERM switch in with the TERM switch in the IN position the mod ule is internally terminated and the pressure is diverted from the pipette into a dummy load Set the Mode Selector Switch to CONT operation Select the pressure units switch to either psig or Kg cm2 position Adjust the pressure regulator to the desired pressure level and note the 3 digit pressure display Set the Eject and Pause time settings for the desired timing Select the desired mode setting to
14. either cycle or other mode of operation Release TERM switch by pushing and releasing the TERM switch pushbut ton The pressure is now directed into the pipette To monitor analog output connect the polygraph recorder to the Analog Output terminal thus the analog of pressure 0 to 1000 mV full scale in the 100 psi position or 700 mV full scale in the Kg cm2 position will be recorded PNEUMATIC PUMP PPM 2 psig kg cm container PRESSURE pressure gauge pressure regulator output _____ pressure gauge PRESSURE REGULATOR CYCLETRIGG GATE CONT TERM 1 8 Diam e flexible tubing EJECT TIME PAUSE Se Use nitrogen or air as a pressure source Do not use combustible media such as oxygen or propane Alternate pressure source can ve an air compressor with a storage tank Input pressure source should not exceed 50 psi 3 2 kg cm OUT ANALOG SYNC s peTTE To pipette Publication 5403 003 REV B 3 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual PPM 2 Gas Input Connections Use nitrogen or air as a pressure source Do not use combustible media such as oxy gen or propane Alternate pressure source can be an air compressor with a storage tank Input pressure source should not exceed 50 psi 3 2 kg cm
15. electrode pipette sum of retention and ejection current Switch in either pos or neg megaohms position digital display reads actual electrode barrel resistance in megaohms derived by passing positive or negative constant current 50nA through elec trode pipette Digital display flashes whenever electrode barrel resistance exceeds working range of current pump i e when electrode resistance times current exceeds compliance of 105V Depressing switch causes digital display to read voltage across pipette BM 2 Balance Module Neutralization Bal Max 2500 nA automatically controlled Pump Range Current Pump Compliance 105 V linear constant current source manually adjust 0 to 500 nA by pump control polarity selected OFF switch Dig Meter Display 3 digits and sign Publication 5403 003 REV B s Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Specifications Specifications Cont d UNBALANCE CURRENT CURRENT PUMP SWITCH Unbalance Current Digital display reads unbalance ground going current in nA in Mode Current Pump Mode Single Cycle Recycle Time Unit Switch Inputs Analog Input Analog Output Sync Output Output Connector PPM 2 Module this mode automatic current neutralization provided Auto balance feature is switched off display reads amount of current in nanoamperes passed through balance barrel as adjusted by Pump control In single cycle mo
16. ion of a desired tip configuration Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Alignment Procedure a bo eee a 4 IL AUTOMATIC BALANCING SYSTEM ni h ap ap i AUTOMATIC BALANCING SYSTEM BH 2 Balance Module The BH 2 System is designed to provide an Automatic Balancing feature in order to null out any voltage that may appear due to retention and or I Desc Mili ejection at the cell junction Needless to say when a system is dispatched the automatic balancing controls is precisely adjusted at the factory and as such will track pump current 500 nA retention and or ejection accurately and balance well within 1 nA at full scale However in the field situations may arise whereby an IP 2 BH 2 Balance Module pump module is added and realignment of the balancing may become necessary To accommodate this eventuality the realignment procedure as outlined here and should be followed Publication 5403 003 REV B 4 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Alignment Procedure IP 2 Module Alignment and Control Settings Test Conditions 1 Moeau N Only one IP 2 module powered at one time Retention Fully CCW counterclockwise Fjection Fully CCW Current Resistance switch in nA position Gate push button in Term push button in IP 2 Module Adjustment Set R133 dower pin to OV zero voltage
17. l location CR114 hole 7 o clock to 4 500V 0 1V by adjusting VR 106 Set VR 110 unlabeled to obtain 1 ms pulse train at pin 1 U 30 4 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Alignment Procedure Final Adjustment and Control Setting Test Conditions 1 2 3 4 All IP 2 s and BAL module output connected to common point through 50 All IP 2 modules in CONT mode Retention and Ejection fully CCW TERM push button out Mohm resistor This point tied to ground through an RT 50 Mohm resistor Oscilloscope connected across RT Making effective RT Scope approximate ly equal to 1 Mohm Oscilloscope setting Horizontal 5 ms division line sync Vertical 5mV divi sion Adjusting Procedure BM 2 Balance Module a b C Adjust VR 103 to obtain OV zero voltage on CRO Adjust VR 104 and or VR 105 to obtain 000 Bal display Set unbalance curr current pump switch to current pump position Set VR 108 to obtain OV on CRO Set VR109 to get 001 reading on balance display Return unbalance cur rent current pump switch to unbalance current position Adjusting Procedure IP 2 Module a ae o Select one IP 2 module and turn Ejection control fully clockwise Correct DC offset as measured by CRO by adjusting VR 101 until OV reading is reached Reverse ejection polarity and correct DC offset on CRO with VR 101 when necessary Return
18. led eject and injection current Cautions Do not remove any modules while the power in ON e When a module is removed ascertain that same is placed in a non electrostatic or grounded environment since electrostatic charges may damage some of the inte grated circuits Allow minimum equipment warm up time of 15 minutes prior to use e When measuring electrode resistance it is recommended that the electrode resistance values be measured one module at a time In other words select the positive or negative position on the Current Resistance Selector Switch 2a page 9 and after the resistance measurement has been completed return the switch to the center nA position e It is recommended that the resistance measurement be made with the auto bal ance switch 3b page 18 on the BM 2 balance module set in the UNBAL cur rent position It is noteworthy that when the instrument is set up for automatic current balance switch set in UNBAL CURR position on Auto Bal Current Selector 3b page 6 the current used for electrode resistance Measurement 50 nA is automatically balanced and as such prevents any ground going currents at the cell junction It is therefore required that the current be measured one at a time to prevent any feedback between resistance current channels In order to operate this system it is required that the OUTPUT 13b page 6 be terminated into an external load This applies specifically to the balance cha
19. mitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This temperature controller is not registered with the FDA and is not for clinical use on human patients AN CAUTION Not for clinical use on human p
20. nnel since no internal dummy load is provided In other words the Micropipette balance barrel when connected provides such a termination Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Specifications Specifications MSC 2 Power Supply Outputs IP 2 Module Current Pump Ejection Current Ejection Indicator Retention Current 125 V 0 1 Amps 15 V 0 5 Amps 5 V 3Amps line operated 115 220 VAC 50 60 Hz Compliance 105 V linear constant current source Pulsing controlled by ejection timing module switch amplitude adjustable by 10 turn ejection control and range switch from 0 50 or 0 500 nA polarity selected by polarity switch accuracy 1 nA Red LED lamp indicates eject time period green LED lamp indi cates pause time period Amplitude adjustable by front panel dial from 0 50 nA polarity is automatically set opposite to ejection current polarity Analog Input Lemo miniature receptacle ground referenced 5mV nA input impedance 100 kQ Analog Output Lemo miniature receptacle ground referenced 5mV nA Sync Output ae miniature receptacle TTL pulse time incident with eject pulse CURRENT AND RESISTANCE Metering System Digital meter display 3 digits and sign RESISTANCE CURRENT SWITCH Current Mode Resistance Mode No Compliance Indicator Voltage Readout Switch Switch in center nA position digital display reads total current in nA passed through the micro
21. oring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 5 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Table of Contents SUBJECT PAGE NO Warranty and Repair Information Manual Description cceeeeeeeeeeeeeeeeeeeees 2 Warranty eirian eee eae aia 2 Repair Facilities and Parts ccccssscceeeeeeeees 2 Serial Numbers viene tie Sate ae atier dues Celene 2 Introduction Features erinti arene earra aan EN deda 3 CAUTIONS wiceaicaccsstasstetcneecncen te teeaiassiieoncen Hin eebeeneteies 3 Specifications secctccccesicestsacetecticttentccascassaaseansiende 4 5 System Description BH 2 Mainframe and Balance Module 6 MS 2 Power Supply ccacccimeticanratiiucaien 6 IP 2 Pump Module ssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 7 8 PPM 2 Pneumatic Pump Module ssssssssnnnnnnnnnnnnnn 9 Recommended Setup Procedure Electrode Resistance Measurement 10 Setting of Retention Current ceseseeees 11 Setting of Ejection Current cceee 11 Setup of Automatic Balance cccceeeee 11 Setup of Pneumatic Pressure Module
22. ource a selectable jumper is provided 1b Balance current digital display 3 digit amp sign 3b Automatic balance and current pump selector 2b Pump current control 4b Pump current polarity 0 500 nA 10 turn Pot selector 5b Manual Start and 6b Trigger input Start Stop Control and Stop Terminals 8b Single cycle and recycle selector switch 9b Time Clock Selector 7b Trigger Gate Input 10b Sync Output Terminal Terminals 12b Analog output terminal 5 mV nA 11b Analog input terminal 5 mV nA 13b Micropipette Current Drive Output Connector Publication 5403 003 REV B Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual IP 2 Pump Module The system housing mainframe can accommodate up to five IP 2 pump modules and as such will accommodate a seven barrel pipette to handle five drug one balance and one recording barrel Each IP 2 pump module includes control for precise settings of current magnitude and polarity retention 0 to 50 nanoamperes ejection 0 to 500 nanoamperes The actual current and polarity is continuously displayed digitally and can be externally monitored at the analog output terminal Note The system can also accommodate up to 5 any combination of pneumatic and iontophoretic mod ules and thus provide full control of simultaneous pneumatic and iontophoretic ejection of drugs Ejection Timing and Mode Switch The mode switch provides five push button con
23. p to 1 5 hours Figure 6 Typical results illustrating spontaneous Purkinje cell discharges recorded with MSC multibarrel pipette Publication 5403 003 REV B Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Seven Barrel Micropipette Model MS 7PB 2mm Scale 2 1 10cm The MS 7PB pipette has been designed for iontophoretic use as well as pneumatic ejection of drugs and compliments the BH 2 NeuroPhore System The standard blank consists of seven barrels pyrex Omegadot tubing 1 5 mm O D and 1 2 mm LD prepulled to 2 mm formation as shown The design of the pipette readily lends itself for electrical connection by inserting Ag wire or tube attach ment for pneumatic use The overall length of the blank assembly is 10 centimeters and fits the Narishige or an equivalent electrode puller The pipette can be supplied in either configuration prepulled blanks as shown or in a pulled micron tip format The micron tip is not bumped and therefore can be finalized prior to use The orifice ratio of each barrel is 0 80 and thus is optimized for mini mal tip resistance when pulled to micron size Omegadot tubing per mits easy filling Fanned out outer barrel assembly provides separation thus avoiding leakage due to spills between barrels The 22 millimeter long twisted segment of the blank assembly assures singular tip forma tion during pulling action and pro vides flexibility in select
24. rom the micropipette preparation into an internal dummy load 100 megohms This function is particularly useful for testing of possible instability in the preparation pipette Analog Input The analog input terminal is available to facilitate externally controlled current pump ing action An external voltage applied to the input will generate a pumping current at a ratio of 5 millivolt nanoampere This current will be summated with any preset pump current governed by both the Retention and Eject controls The combined mag nitude and sign of the summated pumping current is displayed on the digital display of each corresponding pump module This input can be connected to a computer D A converter when external programming is desired IP 2 Pump Module 4 Analog Output The analog output terminal provides a buffered voltage which is proportional in mag nitude and polarity to the actual current passed from the current pump into the pipette The conversation ratio is 5 mV nA This output can be polygraphically record ed to monitor progress of the experiment Sync Output Terminal The sync output provides a TTL pulse that coincides with the eject time This output is provided to trigger external devices such as computer event counter etc tONTOPHORESIS 1a Pump Current Electrode Resistance Digital Display 3 digits and sign 2a Pump Current Electrode 5a Ejection Current Selector Resistance selector 0 50 nA to 0 500 nA
25. since the eject time func tion is slaved to the duration of the gate input Continuous Mode When the Cont switch is energized the eject pump pressure is continuously main tained Termination Mode When the Term switch is energized the output pressure is automatically diverted from the preparation into an internal Dummy Load This function is particularly useful for setting up the desired pressure range and timing while preventing ejection of any drug from the pipette 3 Harvard Apparatus Medical Systems BH 2 Micro lontophoresis System User s Manual Recommended Setup Procedure Prior to turning power ON set push in the TERM 100 M Switch 9A p 8 on each of the pump modules thus terminating each pump into the internal 100 M Load a Turn all Retention controls GA p 8 and Eject controls 6A p 8 to zero fully counter clockwise b Connect the reparation to current drive output cable connector 13B p 6 Balance Module Front Panel Connector J11 Balance Module P C Board Ground Black Pin 7 A IP 1 Pump Module Brown 1 G IP 2 Pump Module Red 2 F IP 3 Pump Module Orange 3 E IP 4 Pump Module Yellow 4 D IP 5 Pump Module Green 5 C Balance Module Blue 6 B c Turn power switch ON d Mount the filled pipette into the micromanipulator assembly e Connect the electrical and or pneumatic connections to the pipette f Advance the pipette into the outer layer of tissue mainly to establish contact bet
26. trols which include operations such as Cycle Trigger Gate Continuous and Termination Cycle Mode In the cycle mode by virtue of selecting single or recycle operation on the BH 2 mod ule an incoming trigger or cycle start push button will initiate the ejection pumping action In this mode each succeeding pump module is automatically triggered after the pause time of the preceding event has been completed Both EJECT and PAUSE times can be preset to cover a range from 10 to 990 milliseconds with a 10 millisec ond resolution and 1 to 99 seconds with a 1 second resolution Trigger Mode When the TRIG switch is energized the eject time interval will be started by virtue of the respective incoming trigger pulse applied to the input panel of the BH 2 balance module Eject timing interval can be preset covering a range form 10 to 990 millisec onds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second resolution Gate Mode When the Gate switch is energized the eject current will be started by virtue of a gate input signal provided to the respective TRIG GATE terminals on the BH 2 balance module The Eject Pause time settings are automatically disengaged in this mode since the Eject time is slaved to the duration of the gate input Continuous Mode When the CONT switch is energized the ejection pump current is continuously main tained Termination Mode When the TERM switch is energized the output is automatically diverted f
27. ween pipette tip and tissue fluid g Release the TERM switch by releasing the TERM switch the pipette is electri cally pneumatically coupled to the apparatus Electrode Resistance Measurement Electrode resistance measurement is achieved by setting the switch Pump current and electrode resistance selector 2A p 8 into positive or negative MQ setting Ascertain that TERM 100 MQ internal dummy load resistor mode switch A p 8 is released Measure the electrode resistance by immersing the tip of the micropipette at the ini tial stage of entry into the tissue or a droplet of saline to establish the resistance value of each barrel with ground reference Ascertain appropriate resistance polarity elec trode resistance selector 2A p 8 that coincides with ejection current polarity for each drug contained in the barrel Resistance value for each barrel should be measured one at a time The selection of the appropriate polarity of the resist ance selector switch positive or negative to cor respond to the cathodic or anodic ion composition of the drug is vital Inasmuch as during the resistance measurement the resistance metered circuit will generate a con tinuous 50 nanoamperes of current it is advisable that the polarity of this current be such to eject the drug ions thus avoiding the possibility of tissue or other particles from the surrounding fluid to clog the barrel It is also suggested that the front han dle bar handles on

Neurophore BH-2 Mico-Iontophoresis System User`s Manual

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